Prupe.4G034100_v2.0.a1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000662
Physical Location & Seq
Forward (+)
1586053 .. 1590183
4131 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G034100.1

Sequence Viewer

Length: 2160 bp
ATGGTTTCCTCCATAAATCCCATTCCTACAAGCGTCAAGAAGCTATGGGAAAGGTGGAACCTCCGGAGCTTTATCCTCTTAAGCCTCACATTGCAGACCATTTTAATTCTTGGTGCACCCTTCAGAAAGCGAGCACCCAACATGGCGATAATCTTCATCATCTGGTCATCTTATTTGCTGGCTGACTGGGCTGCTAACTTTGCAATTGGGTTAATCTCAAACAGCCAAGGCAATGCTCGAGGTACAGGCGATAACAATGAGGATCTTTTGGCATTTTGGGCTCCATTTCTCTTGTTACACCTTGGTGGTCCAGACACCATTACTGCGTTTGCTCTTGAAGATAACACGCTGTGGCTAAGGCATTTTCTTGGCCTCATATTCCAAGTTATAGCTGCCATATATGTCTTCATTCAATCATTTCCCACCAACAAGCTGTGGCCATCAACAATCCTCCTGTTTCTGGCAGGAACCATCAAGTATGCTGAGCGCACACGCGGTTTGTATCTTGCAAGCTTGGACAATTTCAAGGAATCCATGCTTAAAAAACCTGACCCCGGACCAAATTATGCAAAGCTCATGGAGGAGTACTCCTCAAAAAAAGAAGCCAAACTTCCAACTCATATAGAGTTGACAGCAGAACGCAGCAAAGAATCCAGGACAGTAACTTATGTTGCGGAACCAGGTGATATGGAGAATGATATTGCAATGGTCCGACATGCTTACCACTTCTATAAAATCTTCAGGGGTCTTATTGTGGATCTCATTTTTAGCTTCCACGAACGCTTTGAGAGCCGTGCATTTTTCCAAGAAAGAGAGGCAAAAGATGCGTTTAGATTGATTGCAATTGAGCTCAACTTTGTATATGAAGCTCTCTTCACCAAGGCTGTTGTGGTGCATTCTATGCGAGGATGCATTTTCCGGGCTATATCCTTCACTGCGGTTTCCATAGCCCTTGGTTTCTTCTATAAACTAGAGAAACATGATTACCATAAGTTTGATGTTGGAATTACCTATACCCTGCTTTTTGGTGCTTTAGGCCTGGATTCAATAGCTCTTTTCATGCTTATATTCTCCGATTGGACTGTCGCAGCTCTGACGAAATCTTGGCAAAAATCTTTTGTCGCTACTATTCTAAAAAAGTACCTCAGTTTCAAGAGACCAAGTTGGTCTGAAAATACAAGTTGTTTGGACTGGATTAGACAAATCCTGTTTCGAAGGTGGTATGAATCCATCTCTACATTCAACTTGATAGATTATTCCCTCAAGGAACGCGAAAAAATGTTTCCTAATATTTTTGACTACCCTGGTATTGCCTACATTAAAATCATCGATCTCTTGGGCCTCAAGGATCTTCGAGATAAAATGAAATATAGGCACAGCACACCACTCACTAGAGCGTTATGGGAATTCATTTTTAAGGAGTTGCAAAGTAAATCCATGCTTGCAGATGATCCTGAAACAGCCAAGAGAATATGTTCGGCAAGAGGTGACTGGGTTCTCCAGGATAGTGATTGGAATAGTACTGAACACAGTACCTTTCTACGATATATTGTTGAAGTTGATTATGACCAGAGCATTCTATTGTGGCACATTGCTACTGAGTTCTGCTATAACATGGAGTGGAGCGATGGAGATGGAGATGGCTGTAAAATCAGTAAGGCCCTGTCGGATTATATGTTATATCTCTTAGTTATGCAGCCCACTTTGACGTCTTCTGTAGCAGGAATTGGACAAATAAGATTTCGGGATACGTGTGCAGAAGCCAAAAAATTCTTTAGCAGAAGAGAACTTGGGTCAGGAAGATCAGGTGGAGATGAACAACAACAAGAGGCCTGTCAGAGAATCCTTGAGGTGAATACCGAAGTTGAACCTGTTGCTGTCAAAGGGGATAGAAGTAAATCCGTTCTGTTTGATGCATGTATTTTGGCCAAAAAATTGATGGACTTGAAGGAAAAGAAATGGGAGCTAATGAGCAAAGTATGGGTGGAACTGCTCTCATATACTGCTGGTCATTGCAGAGCAAATGACCATGTTCAATTGCTGAGCAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTATTGATGGCTCATTTTGGCATAGGGGAGCAATTCCAAATAAATGAGGGCCATGCTAGAGCAAAACTCATTGTGGGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

720

Amino Acids

82.66

Weight (kDa)

8.33

Isoelectric Point (pI)

39.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1712
AccII CGCG 2 cut(s) 495, 1272
AccIII TCCGGA 1 cut(s) 63
AciI CCGC 3 cut(s) 495, 674, 938
AclWI GGATC 4 cut(s) 270, 765, 1356, 1445
AcoI YGGCCR 2 cut(s) 437, 1926
AcsI RAATTY 2 cut(s) 1406, 1769
AcuI CTGAAG 2 cut(s) 106, 724
AcyI GRCGYC 1 cut(s) 1709
AdeI CACNNNGTG 1 cut(s) 351
AfaI GTAC 5 cut(s) 244, 587, 1142, 1522, 1534
AfiI CCNNNNNNNGG 2 cut(s) 460, 554
AflII CTTAAG 1 cut(s) 79
AflIII ACRYGT 1 cut(s) 1751
AjnI CCWGG 5 cut(s) 653, 679, 1038, 1303, 1500
AleI CACNNNNGTG 1 cut(s) 303
Alw21I GWGCWC 3 cut(s) 118, 136, 852
Alw26I GTCTC 1 cut(s) 1150
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 4 cut(s) 270, 765, 1356, 1445
Ama87I CYCGRG 1 cut(s) 237
Aor13HI TCCGGA 1 cut(s) 63
AoxI GGCC 8 cut(s) 370, 437, 1036, 1339, 1659, 1830, 1926, 2125
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 5 cut(s) 191, 392, 642, 1088, 1696
ApoI RAATTY 2 cut(s) 1406, 1769
Asp700I GAANNNNTTC 1 cut(s) 1474
AspLEI GCGC 1 cut(s) 489
AspS9I GGNCC 6 cut(s) 308, 557, 709, 1339, 1660, 2125
AsuC2I CCSGG 2 cut(s) 555, 920
AsuHPI GGTGA 4 cut(s) 695, 868, 1499, 1864
AsuII TTCGAA 1 cut(s) 1213
AvaI CYCGRG 1 cut(s) 237
AvaII GGWCC 3 cut(s) 308, 557, 709
BaeGI GKGCMC 1 cut(s) 118
BalI TGGCCA 2 cut(s) 439, 1928
BanII GRGCYC 2 cut(s) 283, 852
BbsI GAAGAC 2 cut(s) 397, 1704
Bbv12I GWGCWC 3 cut(s) 118, 136, 852
BbvI GCAGC 5 cut(s) 178, 379, 654, 1100, 1708
BccI CCATC 8 cut(s) 448, 479, 1238, 1622, 1628, 1634, 1933, 2077
BceAI ACGGC 1 cut(s) 777
BciT130I CCWGG 5 cut(s) 655, 681, 1040, 1305, 1502
BciVI GTATCC 1 cut(s) 1741
BcnI CCSGG 2 cut(s) 555, 920
BcoDI GTCTC 1 cut(s) 1150
BfaI CTAG 3 cut(s) 971, 1392, 2133
BfmI CTRYAG 1 cut(s) 1716
BfrI CTTAAG 1 cut(s) 79
BfuI GTATCC 1 cut(s) 1741
BisI GCNGC 5 cut(s) 192, 393, 643, 1089, 1697
BlpI GCTNAGC 2 cut(s) 483, 2042
BlsI GCNGC 5 cut(s) 193, 394, 644, 1090, 1698
BmcAI AGTACT 2 cut(s) 587, 1522
Bme1390I CCNGG 7 cut(s) 555, 655, 681, 920, 1040, 1305, 1502
Bme18I GGWCC 3 cut(s) 308, 557, 709
BmeT110I CYCGRG 1 cut(s) 237
BmgT120I GGNCC 6 cut(s) 308, 557, 709, 1339, 1660, 2125
BmiI GGNNCC 4 cut(s) 59, 282, 469, 678
BmrFI CCNGG 7 cut(s) 555, 655, 681, 920, 1040, 1305, 1502
BmrI ACTGGG 2 cut(s) 196, 1501
BmsI GCATC 3 cut(s) 814, 899, 1903
BmuI ACTGGG 2 cut(s) 196, 1501
BpiI GAAGAC 2 cut(s) 397, 1704
BplI GAGNNNNNCTC 6 cut(s) 572, 604, 575, 607, 2127, 2159
BpmI CTGGAG 1 cut(s) 1484
Bpu10I CCTNAGC 1 cut(s) 356
Bpu1102I GCTNAGC 2 cut(s) 483, 2042
Bpu14I TTCGAA 1 cut(s) 1213
BpuEI CTTGAG 3 cut(s) 1247, 1328, 1868
BpuMI CCSGG 2 cut(s) 555, 920
Bsa29I ATCGAT 1 cut(s) 1329
BsaAI YACGTR 1 cut(s) 1752
BsaHI GRCGYC 1 cut(s) 1709
BsaI GGTCTC 1 cut(s) 1150
BsaJI CCNNGG 6 cut(s) 226, 301, 553, 879, 952, 1303
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 2046, 2076
Bsc4I CCNNNNNNNGG 2 cut(s) 460, 554
Bse1I ACTGG 3 cut(s) 191, 1196, 1496
Bse3DI GCAATG 5 cut(s) 89, 238, 711, 1590, 2011
BseAI TCCGGA 1 cut(s) 63
BseBI CCWGG 5 cut(s) 655, 681, 1040, 1305, 1502
BseCI ATCGAT 1 cut(s) 1329
BseDI CCNNGG 6 cut(s) 226, 301, 553, 879, 952, 1303
BseGI GGATG 1 cut(s) 914
BseLI CCNNNNNNNGG 2 cut(s) 460, 554
BseMI GCAATG 5 cut(s) 89, 238, 711, 1590, 2011
BseMII CTCAG 4 cut(s) 474, 1159, 1590, 2033
BseNI ACTGG 3 cut(s) 191, 1196, 1496
BseRI GAGGAG 2 cut(s) 580, 596
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 5 cut(s) 178, 379, 654, 1100, 1708
BsgI GTGCAG 1 cut(s) 1776
Bsh1236I CGCG 2 cut(s) 495, 1272
BshFI GGCC 8 cut(s) 372, 439, 1038, 1341, 1661, 1832, 1928, 2127
BshVI ATCGAT 1 cut(s) 1329
BsiHKAI GWGCWC 3 cut(s) 118, 136, 852
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 3 cut(s) 64, 555, 919
BslI CCNNNNNNNGG 2 cut(s) 460, 554
BsmAI GTCTC 1 cut(s) 1150
BsmI GAATGC 2 cut(s) 895, 1575
BsnI GGCC 8 cut(s) 372, 439, 1038, 1341, 1661, 1832, 1928, 2127
Bso31I GGTCTC 1 cut(s) 1150
BsoBI CYCGRG 1 cut(s) 237
Bsp119I TTCGAA 1 cut(s) 1213
Bsp1286I GDGCHC 4 cut(s) 118, 136, 283, 852
Bsp13I TCCGGA 1 cut(s) 63
Bsp143I GATC 6 cut(s) 262, 757, 1330, 1348, 1450, 1802
Bsp1720I GCTNAGC 2 cut(s) 483, 2042
BspACI CCGC 3 cut(s) 495, 674, 938
BspANI GGCC 8 cut(s) 372, 439, 1038, 1341, 1661, 1832, 1928, 2127
BspCNI CTCAG 4 cut(s) 475, 1158, 1591, 2034
BspDI ATCGAT 1 cut(s) 1329
BspEI TCCGGA 1 cut(s) 63
BspFNI CGCG 2 cut(s) 495, 1272
BspLI GGNNCC 4 cut(s) 59, 282, 469, 678
BspPI GGATC 4 cut(s) 270, 765, 1356, 1445
BspT104I TTCGAA 1 cut(s) 1213
BspTI CTTAAG 1 cut(s) 79
BspTNI GGTCTC 1 cut(s) 1150
BsrDI GCAATG 5 cut(s) 89, 238, 711, 1590, 2011
BsrI ACTGG 3 cut(s) 191, 1196, 1496
BssECI CCNNGG 6 cut(s) 226, 301, 553, 879, 952, 1303
BssMI GATC 6 cut(s) 262, 757, 1330, 1348, 1450, 1802
BssNI GRCGYC 1 cut(s) 1709
BssT1I CCWWGG 4 cut(s) 226, 301, 879, 952
Bst2UI CCWGG 5 cut(s) 655, 681, 1040, 1305, 1502
Bst4CI ACNGT 3 cut(s) 661, 1084, 1532
Bst6I CTCTTC 2 cut(s) 878, 1777
BstACI GRCGYC 1 cut(s) 1709
BstAFI CTTAAG 1 cut(s) 79
BstAPI GCANNNNNTGC 2 cut(s) 824, 901
BstBAI YACGTR 1 cut(s) 1752
BstBI TTCGAA 1 cut(s) 1213
BstC8I GCNNGC 4 cut(s) 132, 180, 511, 1443
BstDEI CTNAG 6 cut(s) 356, 483, 1145, 1599, 1687, 2042
BstF5I GGATG 1 cut(s) 914
BstFNI CGCG 2 cut(s) 495, 1272
BstHHI GCGC 1 cut(s) 489
BstKTI GATC 6 cut(s) 265, 760, 1333, 1351, 1453, 1805
BstMAI GTCTC 1 cut(s) 1150
BstMBI GATC 6 cut(s) 262, 757, 1330, 1348, 1450, 1802
BstMWI GCNNNNNNNGC 6 cut(s) 188, 200, 278, 789, 824, 901
BstNI CCWGG 5 cut(s) 655, 681, 1040, 1305, 1502
BstNSI RCATGY 2 cut(s) 719, 1920
BstSCI CCNGG 7 cut(s) 553, 653, 679, 918, 1038, 1303, 1500
BstSFI CTRYAG 1 cut(s) 1716
BstSLI GKGCMC 1 cut(s) 118
BstUI CGCG 2 cut(s) 495, 1272
BstV1I GCAGC 5 cut(s) 178, 379, 654, 1100, 1708
BstV2I GAAGAC 2 cut(s) 397, 1704
BstX2I RGATCY 3 cut(s) 262, 757, 1348
BstYI RGATCY 3 cut(s) 262, 757, 1348
Bsu15I ATCGAT 1 cut(s) 1329
BsuI GTATCC 1 cut(s) 1741
BsuRI GGCC 8 cut(s) 372, 439, 1038, 1341, 1661, 1832, 1928, 2127
BsuTUI ATCGAT 1 cut(s) 1329
BtgZI GCGATG 1 cut(s) 1641
BtsCI GGATG 1 cut(s) 914
BtsI GCAGTG 1 cut(s) 933
BtsIMutI CAGTG 1 cut(s) 933
Cac8I GCNNGC 4 cut(s) 132, 180, 511, 1443
CfoI GCGC 1 cut(s) 489
Cfr13I GGNCC 6 cut(s) 308, 557, 709, 1339, 1660, 2125
ClaI ATCGAT 1 cut(s) 1329
CseI GACGC 1 cut(s) 22
CsiI ACCWGGT 1 cut(s) 679
Csp6I GTAC 5 cut(s) 243, 586, 1141, 1521, 1533
CviQI GTAC 5 cut(s) 243, 586, 1141, 1521, 1533
DdeI CTNAG 6 cut(s) 356, 483, 1145, 1599, 1687, 2042
DpnI GATC 6 cut(s) 264, 759, 1332, 1350, 1452, 1804
DpnII GATC 6 cut(s) 262, 757, 1330, 1348, 1450, 1802
DraIII CACNNNGTG 1 cut(s) 351
EaeI YGGCCR 2 cut(s) 437, 1926
Eam1104I CTCTTC 2 cut(s) 878, 1777
EarI CTCTTC 2 cut(s) 878, 1777
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 4 cut(s) 226, 301, 879, 952
Eco147I AGGCCT 2 cut(s) 1038, 1832
Eco24I GRGCYC 2 cut(s) 283, 852
Eco31I GGTCTC 1 cut(s) 1150
Eco47I GGWCC 3 cut(s) 308, 557, 709
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 2 cut(s) 106, 724
Eco88I CYCGRG 1 cut(s) 237
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 1 cut(s) 1660
EcoRI GAATTC 1 cut(s) 1406
EcoRII CCWGG 5 cut(s) 653, 679, 1038, 1303, 1500
EcoT14I CCWWGG 4 cut(s) 226, 301, 879, 952
EcoT22I ATGCAT 2 cut(s) 914, 1918
EcoT38I GRGCYC 2 cut(s) 283, 852
ErhI CCWWGG 4 cut(s) 226, 301, 879, 952
FalI AAGNNNNNCTT 2 cut(s) 594, 626
Fnu4HI GCNGC 5 cut(s) 192, 393, 643, 1089, 1697
FokI GGATG 1 cut(s) 921
FriOI GRGCYC 2 cut(s) 283, 852
Fsp4HI GCNGC 5 cut(s) 192, 393, 643, 1089, 1697
FspBI CTAG 3 cut(s) 971, 1392, 2133
GlaI GCGC 1 cut(s) 488
GluI GCNGC 5 cut(s) 192, 393, 643, 1089, 1697
GsuI CTGGAG 1 cut(s) 1484
HaeIII GGCC 8 cut(s) 372, 439, 1038, 1341, 1661, 1832, 1928, 2127
HapII CCGG 3 cut(s) 64, 555, 919
HgaI GACGC 1 cut(s) 22
HhaI GCGC 1 cut(s) 489
Hin1I GRCGYC 1 cut(s) 1709
Hin6I GCGC 1 cut(s) 487
HinP1I GCGC 1 cut(s) 487
HincII GTYRAC 1 cut(s) 630
HindII GTYRAC 1 cut(s) 630
HindIII AAGCTT 1 cut(s) 511
HinfI GANTC 5 cut(s) 530, 650, 1043, 1226, 1842
HpaII CCGG 3 cut(s) 64, 555, 919
HphI GGTGA 4 cut(s) 695, 868, 1499, 1864
Hpy166II GTNNAC 2 cut(s) 116, 630
Hpy188I TCNGA 7 cut(s) 125, 713, 1075, 1095, 1171, 1669, 1839
Hpy188III TCNNGA 9 cut(s) 37, 64, 311, 335, 1153, 1355, 1454, 1745, 1797
Hpy8I GTNNAC 2 cut(s) 116, 630
HpyAV CCTTC 4 cut(s) 130, 940, 1209, 1942
HpyCH4III ACNGT 3 cut(s) 661, 1084, 1532
HpyCH4IV ACGT 2 cut(s) 1709, 1751
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 200, 278, 789, 824, 901
HpyF3I CTNAG 6 cut(s) 356, 483, 1145, 1599, 1687, 2042
HpySE526I ACGT 2 cut(s) 1709, 1751
Hsp92I GRCGYC 1 cut(s) 1709
HspAI GCGC 1 cut(s) 487
Kpn2I TCCGGA 1 cut(s) 63
Kzo9I GATC 6 cut(s) 262, 757, 1330, 1348, 1450, 1802
LmnI GCTCC 5 cut(s) 66, 286, 1623, 1963, 2104
Lsp1109I GCAGC 5 cut(s) 178, 379, 654, 1100, 1708
LweI GCATC 3 cut(s) 814, 899, 1903
MabI ACCWGGT 1 cut(s) 679
MaeI CTAG 3 cut(s) 971, 1392, 2133
MaeII ACGT 2 cut(s) 1709, 1751
MaeIII GTNAC 4 cut(s) 294, 661, 1487, 2062
MalI GATC 6 cut(s) 264, 759, 1332, 1350, 1452, 1804
MboI GATC 6 cut(s) 262, 757, 1330, 1348, 1450, 1802
MfeI CAATTG 3 cut(s) 204, 843, 2036
MflI RGATCY 3 cut(s) 262, 757, 1348
MhlI GDGCHC 4 cut(s) 118, 136, 283, 852
MlsI TGGCCA 2 cut(s) 439, 1928
MluNI TGGCCA 2 cut(s) 439, 1928
MmeI TCCRAC 4 cut(s) 638, 736, 982, 1647
Mox20I TGGCCA 2 cut(s) 439, 1928
Mph1103I ATGCAT 2 cut(s) 914, 1918
MroI TCCGGA 1 cut(s) 63
MroXI GAANNNNTTC 1 cut(s) 1474
MscI TGGCCA 2 cut(s) 439, 1928
MseI TTAA 6 cut(s) 80, 104, 212, 540, 1320, 1416
MslI CAYNNNNRTG 1 cut(s) 303
Msp20I TGGCCA 2 cut(s) 439, 1928
MspCI CTTAAG 1 cut(s) 79
MspI CCGG 3 cut(s) 64, 555, 919
MspR9I CCNGG 7 cut(s) 555, 655, 681, 920, 1040, 1305, 1502
MunI CAATTG 3 cut(s) 204, 843, 2036
Mva1269I GAATGC 2 cut(s) 895, 1575
MvaI CCWGG 5 cut(s) 655, 681, 1040, 1305, 1502
MvnI CGCG 2 cut(s) 495, 1272
MwoI GCNNNNNNNGC 6 cut(s) 188, 200, 278, 789, 824, 901
NciI CCSGG 2 cut(s) 555, 920
NdeII GATC 6 cut(s) 262, 757, 1330, 1348, 1450, 1802
NlaIV GGNNCC 4 cut(s) 59, 282, 469, 678
NmuCI GTSAC 2 cut(s) 1487, 2062
NsiI ATGCAT 2 cut(s) 914, 1918
NspI RCATGY 2 cut(s) 719, 1920
NspV TTCGAA 1 cut(s) 1213
OliI CACNNNNGTG 1 cut(s) 303
PaeR7I CTCGAG 1 cut(s) 237
PceI AGGCCT 2 cut(s) 1038, 1832
PctI GAATGC 2 cut(s) 895, 1575
PdmI GAANNNNTTC 1 cut(s) 1474
PfeI GAWTC 5 cut(s) 530, 650, 1043, 1226, 1842
PfoI TCCNGGA 2 cut(s) 653, 1500
PkrI GCNGC 5 cut(s) 193, 394, 644, 1090, 1698
Ppu21I YACGTR 1 cut(s) 1752
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 5 cut(s) 653, 679, 1038, 1303, 1500
PspGI CCWGG 5 cut(s) 653, 679, 1038, 1303, 1500
PspN4I GGNNCC 4 cut(s) 59, 282, 469, 678
PspPI GGNCC 6 cut(s) 308, 557, 709, 1339, 1660, 2125
PspXI VCTCGAGB 1 cut(s) 237
PsuI RGATCY 3 cut(s) 262, 757, 1348
RsaI GTAC 5 cut(s) 244, 587, 1142, 1522, 1534
RsaNI GTAC 5 cut(s) 243, 586, 1141, 1521, 1533
RseI CAYNNNNRTG 1 cut(s) 303
SacI GAGCTC 1 cut(s) 852
SaqAI TTAA 6 cut(s) 80, 104, 212, 540, 1320, 1416
SatI GCNGC 5 cut(s) 192, 393, 643, 1089, 1697
Sau3AI GATC 6 cut(s) 262, 757, 1330, 1348, 1450, 1802
Sau96I GGNCC 6 cut(s) 308, 557, 709, 1339, 1660, 2125
ScaI AGTACT 2 cut(s) 587, 1522
ScrFI CCNGG 7 cut(s) 555, 655, 681, 920, 1040, 1305, 1502
SduI GDGCHC 4 cut(s) 118, 136, 283, 852
SexAI ACCWGGT 1 cut(s) 679
SfaNI GCATC 3 cut(s) 814, 899, 1903
SfcI CTRYAG 1 cut(s) 1716
Sfr274I CTCGAG 1 cut(s) 237
SfuI TTCGAA 1 cut(s) 1213
SinI GGWCC 3 cut(s) 308, 557, 709
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 5 cut(s) 79, 237, 1262, 1343, 1847
SmoI CTYRAG 5 cut(s) 79, 237, 1262, 1343, 1847
SseBI AGGCCT 2 cut(s) 1038, 1832
SsiI CCGC 3 cut(s) 495, 674, 938
SspI AATATT 1 cut(s) 1291
SspMI CTAG 3 cut(s) 971, 1392, 2133
SstI GAGCTC 1 cut(s) 852
StuI AGGCCT 2 cut(s) 1038, 1832
StyD4I CCNGG 7 cut(s) 553, 653, 679, 918, 1038, 1303, 1500
StyI CCWWGG 4 cut(s) 226, 301, 879, 952
TaaI ACNGT 3 cut(s) 661, 1084, 1532
TaiI ACGT 2 cut(s) 1712, 1754
TaqI TCGA 4 cut(s) 238, 1213, 1329, 1354
TatI WGTACW 2 cut(s) 585, 1520
TfiI GAWTC 5 cut(s) 530, 650, 1043, 1226, 1842
Tru1I TTAA 6 cut(s) 80, 104, 212, 540, 1320, 1416
Tru9I TTAA 6 cut(s) 80, 104, 212, 540, 1320, 1416
TscAI CASTG 1 cut(s) 940
TseFI GTSAC 2 cut(s) 1487, 2062
TseI GCWGC 5 cut(s) 191, 392, 642, 1088, 1696
Tsp45I GTSAC 2 cut(s) 1487, 2062
TspDTI ATGAA 8 cut(s) 145, 397, 879, 1048, 1239, 1379, 1399, 1830
TspGWI ACGGA 1 cut(s) 1891
TspRI CASTG 1 cut(s) 940
Vha464I CTTAAG 1 cut(s) 79
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 3 cut(s) 308, 557, 709
XapI RAATTY 2 cut(s) 1406, 1769
XceI RCATGY 2 cut(s) 719, 1920
XcmI CCANNNNNNNNNTGG 2 cut(s) 886, 1936
XhoI CTCGAG 1 cut(s) 237
XmnI GAANNNNTTC 1 cut(s) 1474
XspI CTAG 3 cut(s) 971, 1392, 2133
ZraI GACGTC 1 cut(s) 1710
ZrmI AGTACT 2 cut(s) 587, 1522
Zsp2I ATGCAT 2 cut(s) 914, 1918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.