Rh6CG067200

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000662
Physical Location & Seq
Forward (+)
6673917 .. 6679558
5642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG067200.1

Sequence Viewer

Length: 2337 bp
ATGGCTTCATTTGCCGCTCCTATTTCCAACGACGTCAAGAAGATCTGGGATAAGTGGAATCTCCGGAGCTTCGTCATATTAAGTCTCACATTGCAGAGCTTCTTAATTCTCTTTGCACCCTTGAGAAAGCGAATGTCGAGCAAATGGGTAACCGTGCCCATCTGGTTAGCTTACTTGCTCGCTGACTGGGCTGCTAACTTCGGTATCGGACTAATTGCAAGCAGCCAAACGAACAGCGATGAAGGTTCCCCAGAGAATGGTAATCTCTTGGCATTCTGGGCTCCCTTCCTTCTTTTGCACCTAGGTGGTCCAGACACCATAATTGCAATTGCTATGGAAGATAATGCGTTGTGGCCAAGGCACTTGCTTGGTCTCATATTCCAGGTTCTAGCTGCAGTGTACGTCTTCGGTGTGCAGTTCTCTGACCAAAACAAGCTACTGTGGCCAACACTCTTGCTGTTTGTACCCGGACTTGTCAAGTATGGCGAGCGAACACGTGCTTTGTATCTTGCCAGCTTGGAAACTCTCAAGGATACCGTATATCCAGTACAGGAACCTCGACTGGATTATGCAAAGTACATGGATGAGTTCTCCGCAAGAAAAGCTGCCAATCTTCCAATTAGTATAAAAATCGAAAAAGGTAGGAGCTCGTATTCGATTTACCGGCTGGGAGAAGGAAACATTGACGATATTACACTAGTGAAGGGTGCTCATTACTTGTTTCAAATCTCCATGGGTCTTATTATAGATCTTGTGTTCAACTATCATCAGCGCAACGAGAGCCGTGCCTATTTCTATACTTTAACATCAGAACAAGCTTTTTCATCAATTACCACCGAGCTCAATTTTATGTATGAAGCTCTGTACACCAAAGCTTATGTGGTACATTCTCGACGGGGATACGTTCTAAGGGCTACATCCTTCATCGCGGTTTGCATAACCCTTGTTTTCTTCTATAGATTAGACAAACATGGCTTTCACAGGTTTGATGTTGGGATTACTTATTCCTTGCTTTTTGGTGCCATAGCTCTTGACTCAATAGCTCTTTCCATGCTGATATTATCTGATTGGACGATCGCTGTTGCATCCAAGTTCTGTAGAAAGTTTCAAGTAGTCACTAGTCTCATGATGAAGTACCTGGAGTTTAAGAGATCATGGGTATCAACTACTACATCAAAGTCTCCCCCAAAAAAAAAAACTACTGCATCAAAGTGTCTGGAGCGGACTAGAAAGATTTTATTTCGAAGATGGTGTGAATCCGTCTCCAGTTTCAGCTTCATAGATTATTCTCTCCGAGAATGTGGACCAGTAGTCGGACCTGACCGTTTTGGCATTTTTTACCGTTGCCGTAGAATTATCAGATTTTTGGGCCACCTGTGCCACAGAATTGTCATATTCCTGGGCCTCAGCTCCCTCAAGTATCATCGAGATGAGCTGAAGTATAGATCTAGCAAGAGACTGGATAAGCACTTGTGGAATTTCATCTTTGAGCAGTTGAAAATTAGATCCTTGGAAATAGGTGATCTTCATGATGCCAAAATTGCTAGAAAAATATGTTCGTCCCAAGGGGATTGGGTTCTCGAAAAGTGTGAGACGCATCCGCATCCGGAGTACATGAACTTAGTAAAGCGCTTTGATCGTGTTGGTTATGACCGACGCGTTCTGATGTGGCATATTGCTACTGAGATGTGCTATTACACAGATGATGATAGGAGCAATATTGAGCATCGTGATGATGATAAAGATGATGATAAGAGCAATATTGAGCATCGTGACTTTAGTAAGACGTTGTCTGATTATATGCTATATCTCTTAATTATGCAGCCTGCTTTGATGTACTCTGTAGCGGGAATCGGACAAAAGAGGTTTAGAGATACGGTTGCAGAAGCCAAAAAATTCTTTAGAAAAAGGGGATTGGGATCCGATGTGAGACTAGACGAGGGAAAACTAGCCGATGCTTGTTGCACACTCCTTGATGTAGATGCAAGAATTGAACCTGGAAATCTCCATGTGGATGGAAGTAAATCTGTTTTGTTTGATGCATGTATTCTGGCCAAGCAGCTGAGGAAATCCGGAGACGAGAAATGGAAATTAATAAGCGCGGTGTGGGTGGATTTGCTATCCTATGCTGCAACTCATTGCAGAGCGCATAGCCACGCGCAATTGCTGACTAAAGGTGGAGAGCTTGTCACCTTTGTTTGGTTACTAATGGCTCGTCTTGGCATTCAGGAGGTCATCCCAGAAATGGAGGACATCCAAGTTCTGAAGGTCGCACCAACAGTAAAACTGATCGTGGAAAAGCAACCTGAAGTATTCAATAAAGTTACTCTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

778

Amino Acids

89.5

Weight (kDa)

9.13

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 429 3.3e-86 Domain of unknown function (DUF4220)
DUF594 PF04578 690 - 742 1.3e-20 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
AccB1I GGYRCC 1 cut(s) 1019
AccB7I CCANNNNNTGG 1 cut(s) 257
AccBSI CCGCTC 2 cut(s) 17, 1222
AccII CGCG 4 cut(s) 929, 1659, 2102, 2159
AccIII TCCGGA 3 cut(s) 63, 1606, 2072
AciI CCGC 7 cut(s) 15, 594, 929, 1222, 1601, 1847, 2102
AclWI GGATC 3 cut(s) 1500, 1914, 1927
AcoI YGGCCR 3 cut(s) 353, 443, 2052
AcsI RAATTY 2 cut(s) 1477, 1895
AcuI CTGAAG 3 cut(s) 1457, 2285, 2328
AcvI CACGTG 1 cut(s) 497
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 9 cut(s) 401, 465, 549, 578, 866, 885, 1136, 1613, 1838
AfeI AGCGCT 1 cut(s) 1631
AfiI CCNNNNNNNGG 4 cut(s) 257, 1313, 2199, 2245
AflIII ACRYGT 2 cut(s) 494, 1657
AgsI TTSAA 6 cut(s) 725, 760, 1109, 1498, 1994, 2317
AhdI GACNNNNNGTC 1 cut(s) 1310
AhlI ACTAGT 2 cut(s) 697, 1118
AjnI CCWGG 4 cut(s) 381, 1137, 1398, 1996
AleI CACNNNNGTG 1 cut(s) 303
Alw21I GWGCWC 3 cut(s) 650, 712, 843
Alw26I GTCTC 9 cut(s) 89, 377, 1127, 1185, 1267, 1450, 1586, 1924, 2070
AlwI GGATC 3 cut(s) 1500, 1914, 1927
Aor13HI TCCGGA 3 cut(s) 63, 1606, 2072
Aor51HI AGCGCT 1 cut(s) 1631
AoxI GGCC 5 cut(s) 353, 443, 1369, 1402, 2052
ApeKI GCWGC 7 cut(s) 191, 222, 392, 605, 1822, 2059, 2129
ApoI RAATTY 2 cut(s) 1477, 1895
AseI ATTAAT 1 cut(s) 2093
AspA2I CCTAGG 1 cut(s) 301
AspLEI GCGC 5 cut(s) 774, 1632, 2102, 2149, 2161
AspS9I GGNCC 5 cut(s) 308, 1304, 1316, 1369, 1402
AsuC2I CCSGG 1 cut(s) 468
AsuHPI GGTGA 2 cut(s) 1532, 2182
AsuII TTCGAA 1 cut(s) 1243
AvaII GGWCC 3 cut(s) 308, 1304, 1316
AvrII CCTAGG 1 cut(s) 301
BaeGI GKGCMC 1 cut(s) 159
BaeI ACNNNNGTAYC 2 cut(s) 187, 220
BalI TGGCCA 3 cut(s) 355, 445, 2054
BamHI GGATCC 1 cut(s) 1919
BanI GGYRCC 1 cut(s) 1019
BanII GRGCYC 3 cut(s) 283, 650, 843
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 397
Bbv12I GWGCWC 3 cut(s) 650, 712, 843
BbvCI CCTCAGC 2 cut(s) 1406, 2063
BbvI GCAGC 7 cut(s) 178, 234, 379, 592, 1834, 2071, 2116
BccI CCATC 3 cut(s) 167, 1242, 2009
BceAI ACGGC 2 cut(s) 768, 1332
BcgI CGANNNNNNTGC 2 cut(s) 767, 801
BciT130I CCWGG 4 cut(s) 383, 1139, 1400, 1998
BciVI GTATCC 2 cut(s) 526, 893
BcnI CCSGG 1 cut(s) 468
BcoDI GTCTC 9 cut(s) 89, 377, 1127, 1185, 1267, 1450, 1586, 1924, 2070
BcuI ACTAGT 2 cut(s) 697, 1118
BfaI CTAG 9 cut(s) 302, 389, 698, 1119, 1227, 1449, 1545, 1934, 1949
BfmI CTRYAG 4 cut(s) 393, 955, 1096, 1842
BfoI RGCGCY 1 cut(s) 1633
BfuI GTATCC 2 cut(s) 526, 893
BglII AGATCT 3 cut(s) 42, 748, 1445
BisI GCNGC 8 cut(s) 15, 192, 223, 393, 606, 1823, 2060, 2130
BlnI CCTAGG 1 cut(s) 301
BlsI GCNGC 8 cut(s) 16, 193, 224, 394, 607, 1824, 2061, 2131
Bme1390I CCNGG 5 cut(s) 383, 468, 1139, 1400, 1998
Bme18I GGWCC 3 cut(s) 308, 1304, 1316
BmeRI GACNNNNNGTC 1 cut(s) 1310
BmgT120I GGNCC 5 cut(s) 308, 1304, 1316, 1369, 1402
BmiI GGNNCC 5 cut(s) 247, 282, 555, 1021, 1921
BmrFI CCNGG 5 cut(s) 383, 468, 1139, 1400, 1998
BmrI ACTGGG 1 cut(s) 196
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 1 cut(s) 397
BpmI CTGGAG 3 cut(s) 1160, 1238, 1249
Bpu10I CCTNAGC 2 cut(s) 1406, 2063
Bpu14I TTCGAA 1 cut(s) 1243
BpuEI CTTGAG 3 cut(s) 142, 512, 1400
BpuMI CCSGG 1 cut(s) 468
BsaAI YACGTR 1 cut(s) 497
BsaBI GATNNNNATC 1 cut(s) 1918
BsaHI GRCGYC 1 cut(s) 33
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 6 cut(s) 301, 356, 732, 1399, 1509, 1564
BsaWI WCCGGW 3 cut(s) 63, 1606, 2072
BsaXI ACNNNNNCTCC 2 cut(s) 2172, 2202
Bsc4I CCNNNNNNNGG 4 cut(s) 257, 1313, 2199, 2245
Bse118I RCCGGY 1 cut(s) 663
Bse1I ACTGG 6 cut(s) 191, 545, 567, 1266, 1307, 1464
Bse3DI GCAATG 2 cut(s) 89, 2137
Bse8I GATNNNNATC 1 cut(s) 1918
BseAI TCCGGA 3 cut(s) 63, 1606, 2072
BseBI CCWGG 4 cut(s) 383, 1139, 1400, 1998
BseDI CCNNGG 6 cut(s) 301, 356, 732, 1399, 1509, 1564
BseGI GGATG 8 cut(s) 589, 917, 1085, 1597, 1603, 2020, 2235, 2253
BseJI GATNNNNATC 1 cut(s) 1918
BseLI CCNNNNNNNGG 4 cut(s) 257, 1313, 2199, 2245
BseMI GCAATG 2 cut(s) 89, 2137
BseMII CTCAG 3 cut(s) 1420, 1674, 2054
BseNI ACTGG 6 cut(s) 191, 545, 567, 1266, 1307, 1464
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 7 cut(s) 178, 234, 379, 592, 1834, 2071, 2116
BseYI CCCAGC 1 cut(s) 667
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 4 cut(s) 929, 1659, 2102, 2159
Bsh1285I CGRYCG 1 cut(s) 1077
BshFI GGCC 5 cut(s) 355, 445, 1371, 1404, 2054
BshNI GGYRCC 1 cut(s) 1019
BsiEI CGRYCG 1 cut(s) 1077
BsiHKAI GWGCWC 3 cut(s) 650, 712, 843
BsiSI CCGG 5 cut(s) 64, 468, 664, 1607, 2073
BslFI GGGAC 1 cut(s) 1546
BslI CCNNNNNNNGG 4 cut(s) 257, 1313, 2199, 2245
BsmAI GTCTC 9 cut(s) 89, 377, 1127, 1185, 1267, 1450, 1586, 1924, 2070
BsmBI CGTCTC 3 cut(s) 1267, 1586, 2070
BsmFI GGGAC 1 cut(s) 1546
BsmI GAATGC 2 cut(s) 272, 2223
BsnI GGCC 5 cut(s) 355, 445, 1371, 1404, 2054
Bso31I GGTCTC 1 cut(s) 377
Bsp119I TTCGAA 1 cut(s) 1243
Bsp1286I GDGCHC 5 cut(s) 159, 283, 650, 712, 843
Bsp13I TCCGGA 3 cut(s) 63, 1606, 2072
Bsp1407I TGTACA 1 cut(s) 864
Bsp19I CCATGG 1 cut(s) 732
BspACI CCGC 7 cut(s) 15, 594, 929, 1222, 1601, 1847, 2102
BspANI GGCC 5 cut(s) 355, 445, 1371, 1404, 2054
BspCNI CTCAG 3 cut(s) 1419, 1675, 2055
BspEI TCCGGA 3 cut(s) 63, 1606, 2072
BspFNI CGCG 4 cut(s) 929, 1659, 2102, 2159
BspHI TCATGA 2 cut(s) 1125, 1528
BspLI GGNNCC 5 cut(s) 247, 282, 555, 1021, 1921
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 3 cut(s) 1500, 1914, 1927
BspT104I TTCGAA 1 cut(s) 1243
BspT107I GGYRCC 1 cut(s) 1019
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 2 cut(s) 17, 1222
BsrDI GCAATG 2 cut(s) 89, 2137
BsrFI RCCGGY 1 cut(s) 663
BsrGI TGTACA 1 cut(s) 864
BsrI ACTGG 6 cut(s) 191, 545, 567, 1266, 1307, 1464
BssAI RCCGGY 1 cut(s) 663
BssECI CCNNGG 6 cut(s) 301, 356, 732, 1399, 1509, 1564
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 5 cut(s) 301, 356, 732, 1509, 1564
Bst2UI CCWGG 4 cut(s) 383, 1139, 1400, 1998
Bst4CI ACNGT 7 cut(s) 154, 441, 538, 1325, 1343, 1879, 2281
BstACI GRCGYC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 864
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 1 cut(s) 1243
BstC8I GCNNGC 5 cut(s) 180, 220, 488, 514, 1827
BstDEI CTNAG 5 cut(s) 908, 1406, 1621, 1683, 2063
BstDSI CCRYGG 1 cut(s) 732
BstEII GGTNACC 1 cut(s) 148
BstF5I GGATG 8 cut(s) 589, 917, 1085, 1597, 1603, 2020, 2235, 2253
BstFNI CGCG 4 cut(s) 929, 1659, 2102, 2159
BstH2I RGCGCY 1 cut(s) 1633
BstHHI GCGC 5 cut(s) 774, 1632, 2102, 2149, 2161
BstMAI GTCTC 9 cut(s) 89, 377, 1127, 1185, 1267, 1450, 1586, 1924, 2070
BstMCI CGRYCG 1 cut(s) 1077
BstMWI GCNNNNNNNGC 8 cut(s) 11, 188, 278, 442, 602, 780, 1377, 1541
BstNI CCWGG 4 cut(s) 383, 1139, 1400, 1998
BstNSI RCATGY 1 cut(s) 2046
BstPI GGTNACC 1 cut(s) 148
BstSCI CCNGG 5 cut(s) 381, 466, 1137, 1398, 1996
BstSFI CTRYAG 4 cut(s) 393, 955, 1096, 1842
BstSLI GKGCMC 1 cut(s) 159
BstUI CGCG 4 cut(s) 929, 1659, 2102, 2159
BstV1I GCAGC 7 cut(s) 178, 234, 379, 592, 1834, 2071, 2116
BstV2I GAAGAC 1 cut(s) 397
BstX2I RGATCY 5 cut(s) 42, 748, 1445, 1505, 1919
BstXI CCANNNNNNTGG 1 cut(s) 2015
BstYI RGATCY 5 cut(s) 42, 748, 1445, 1505, 1919
BsuI GTATCC 2 cut(s) 526, 893
BsuRI GGCC 5 cut(s) 355, 445, 1371, 1404, 2054
BtgI CCRYGG 1 cut(s) 732
BtgZI GCGATG 2 cut(s) 252, 910
BtsCI GGATG 8 cut(s) 589, 917, 1085, 1597, 1603, 2020, 2235, 2253
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 5 cut(s) 180, 220, 488, 514, 1827
CciI TCATGA 2 cut(s) 1125, 1528
CfoI GCGC 5 cut(s) 774, 1632, 2102, 2149, 2161
Cfr10I RCCGGY 1 cut(s) 663
Cfr13I GGNCC 5 cut(s) 308, 1304, 1316, 1369, 1402
CseI GACGC 2 cut(s) 1603, 1665
Csp6I GTAC 9 cut(s) 400, 464, 548, 577, 865, 884, 1135, 1612, 1837
CviQI GTAC 9 cut(s) 400, 464, 548, 577, 865, 884, 1135, 1612, 1837
DdeI CTNAG 5 cut(s) 908, 1406, 1621, 1683, 2063
DriI GACNNNNNGTC 1 cut(s) 1310
EaeI YGGCCR 3 cut(s) 353, 443, 2052
Eam1105I GACNNNNNGTC 1 cut(s) 1310
Ecl136II GAGCTC 2 cut(s) 648, 841
Eco130I CCWWGG 5 cut(s) 301, 356, 732, 1509, 1564
Eco24I GRGCYC 3 cut(s) 283, 650, 843
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 3 cut(s) 308, 1304, 1316
Eco47III AGCGCT 1 cut(s) 1631
Eco53kI GAGCTC 2 cut(s) 648, 841
Eco57I CTGAAG 3 cut(s) 1457, 2285, 2328
Eco72I CACGTG 1 cut(s) 497
Eco91I GGTNACC 1 cut(s) 148
EcoICRI GAGCTC 2 cut(s) 648, 841
EcoO65I GGTNACC 1 cut(s) 148
EcoRII CCWGG 4 cut(s) 381, 1137, 1398, 1996
EcoT14I CCWWGG 5 cut(s) 301, 356, 732, 1509, 1564
EcoT22I ATGCAT 1 cut(s) 2044
EcoT38I GRGCYC 3 cut(s) 283, 650, 843
ErhI CCWWGG 5 cut(s) 301, 356, 732, 1509, 1564
Esp3I CGTCTC 3 cut(s) 1267, 1586, 2070
FalI AAGNNNNNCTT 1 cut(s) 2313
FaqI GGGAC 1 cut(s) 1546
FauI CCCGC 1 cut(s) 1840
Fnu4HI GCNGC 8 cut(s) 15, 192, 223, 393, 606, 1823, 2060, 2130
FokI GGATG 8 cut(s) 596, 904, 1072, 1584, 1590, 2027, 2222, 2240
FriOI GRGCYC 3 cut(s) 283, 650, 843
Fsp4HI GCNGC 8 cut(s) 15, 192, 223, 393, 606, 1823, 2060, 2130
FspBI CTAG 9 cut(s) 302, 389, 698, 1119, 1227, 1449, 1545, 1934, 1949
GlaI GCGC 5 cut(s) 773, 1631, 2101, 2148, 2160
GluI GCNGC 8 cut(s) 15, 192, 223, 393, 606, 1823, 2060, 2130
GsaI CCCAGC 1 cut(s) 671
GsuI CTGGAG 3 cut(s) 1160, 1238, 1249
HaeII RGCGCY 1 cut(s) 1633
HaeIII GGCC 5 cut(s) 355, 445, 1371, 1404, 2054
HapII CCGG 5 cut(s) 64, 468, 664, 1607, 2073
HgaI GACGC 2 cut(s) 1603, 1665
HhaI GCGC 5 cut(s) 774, 1632, 2102, 2149, 2161
Hin1I GRCGYC 1 cut(s) 33
Hin6I GCGC 5 cut(s) 772, 1630, 2100, 2147, 2159
HinP1I GCGC 5 cut(s) 772, 1630, 2100, 2147, 2159
HindIII AAGCTT 2 cut(s) 816, 873
HinfI GANTC 4 cut(s) 58, 1034, 1256, 1851
HpaII CCGG 5 cut(s) 64, 468, 664, 1607, 2073
HphI GGTGA 2 cut(s) 1532, 2182
Hpy166II GTNNAC 3 cut(s) 400, 867, 1304
Hpy8I GTNNAC 3 cut(s) 400, 867, 1304
Hpy99I CGWCG 3 cut(s) 35, 897, 1659
HpyAV CCTTC 7 cut(s) 236, 295, 299, 668, 697, 931, 2260
HpyCH4III ACNGT 7 cut(s) 154, 441, 538, 1325, 1343, 1879, 2281
HpyCH4IV ACGT 5 cut(s) 33, 402, 496, 903, 1787
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 188, 278, 442, 602, 780, 1377, 1541
HpyF3I CTNAG 5 cut(s) 908, 1406, 1621, 1683, 2063
HpySE526I ACGT 5 cut(s) 33, 402, 496, 903, 1787
Hsp92I GRCGYC 1 cut(s) 33
HspAI GCGC 5 cut(s) 772, 1630, 2100, 2147, 2159
Kpn2I TCCGGA 3 cut(s) 63, 1606, 2072
LmnI GCTCC 7 cut(s) 22, 66, 286, 645, 1219, 1415, 1713
Lsp1109I GCAGC 7 cut(s) 178, 234, 379, 592, 1834, 2071, 2116
MaeI CTAG 9 cut(s) 302, 389, 698, 1119, 1227, 1449, 1545, 1934, 1949
MaeII ACGT 5 cut(s) 33, 402, 496, 903, 1787
MaeIII GTNAC 6 cut(s) 148, 1114, 1772, 2188, 2202, 2323
MbiI CCGCTC 2 cut(s) 17, 1222
MboII GAAGA 7 cut(s) 52, 350, 397, 605, 943, 1257, 1517
MfeI CAATTG 2 cut(s) 327, 2162
MflI RGATCY 5 cut(s) 42, 748, 1445, 1505, 1919
MhlI GDGCHC 5 cut(s) 159, 283, 650, 712, 843
MlsI TGGCCA 3 cut(s) 355, 445, 2054
MluI ACGCGT 1 cut(s) 1657
MluNI TGGCCA 3 cut(s) 355, 445, 2054
MlyI GAGTC 1 cut(s) 1028
MmeI TCCRAC 2 cut(s) 51, 1294
MnlI CCTC 8 cut(s) 567, 1415, 1424, 1857, 1933, 2058, 2224, 2242
Mox20I TGGCCA 3 cut(s) 355, 445, 2054
Mph1103I ATGCAT 1 cut(s) 2044
MroI TCCGGA 3 cut(s) 63, 1606, 2072
MscI TGGCCA 3 cut(s) 355, 445, 2054
MseI TTAA 6 cut(s) 80, 104, 803, 1146, 1814, 2093
MslI CAYNNNNRTG 5 cut(s) 303, 1210, 1428, 1731, 2013
Msp20I TGGCCA 3 cut(s) 355, 445, 2054
MspA1I CMGCKG 1 cut(s) 2062
MspI CCGG 5 cut(s) 64, 468, 664, 1607, 2073
MspR9I CCNGG 5 cut(s) 383, 468, 1139, 1400, 1998
MunI CAATTG 2 cut(s) 327, 2162
Mva1269I GAATGC 2 cut(s) 272, 2223
MvaI CCWGG 4 cut(s) 383, 1139, 1400, 1998
MvnI CGCG 4 cut(s) 929, 1659, 2102, 2159
MwoI GCNNNNNNNGC 8 cut(s) 11, 188, 278, 442, 602, 780, 1377, 1541
NciI CCSGG 1 cut(s) 468
NcoI CCATGG 1 cut(s) 732
NlaIV GGNNCC 5 cut(s) 247, 282, 555, 1021, 1921
NmuCI GTSAC 3 cut(s) 1114, 1772, 2188
NsiI ATGCAT 1 cut(s) 2044
NspI RCATGY 1 cut(s) 2046
NspV TTCGAA 1 cut(s) 1243
OliI CACNNNNGTG 1 cut(s) 303
PagI TCATGA 2 cut(s) 1125, 1528
PctI GAATGC 2 cut(s) 272, 2223
PfeI GAWTC 3 cut(s) 58, 1256, 1851
PflFI GACNNNGTC 1 cut(s) 1789
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 8 cut(s) 16, 193, 224, 394, 607, 1824, 2061, 2131
Ple19I CGATCG 1 cut(s) 1077
PleI GAGTC 1 cut(s) 1028
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 1 cut(s) 1028
Ppu21I YACGTR 1 cut(s) 497
PshBI ATTAAT 1 cut(s) 2093
Psp124BI GAGCTC 2 cut(s) 650, 843
Psp6I CCWGG 4 cut(s) 381, 1137, 1398, 1996
PspCI CACGTG 1 cut(s) 497
PspEI GGTNACC 1 cut(s) 148
PspFI CCCAGC 1 cut(s) 667
PspGI CCWGG 4 cut(s) 381, 1137, 1398, 1996
PspN4I GGNNCC 5 cut(s) 247, 282, 555, 1021, 1921
PspPI GGNCC 5 cut(s) 308, 1304, 1316, 1369, 1402
PstI CTGCAG 1 cut(s) 397
PsuI RGATCY 5 cut(s) 42, 748, 1445, 1505, 1919
PsyI GACNNNGTC 1 cut(s) 1789
PvuI CGATCG 1 cut(s) 1077
PvuII CAGCTG 1 cut(s) 2062
RsaI GTAC 9 cut(s) 401, 465, 549, 578, 866, 885, 1136, 1613, 1838
RsaNI GTAC 9 cut(s) 400, 464, 548, 577, 865, 884, 1135, 1612, 1837
RseI CAYNNNNRTG 5 cut(s) 303, 1210, 1428, 1731, 2013
SacI GAGCTC 2 cut(s) 650, 843
SaqAI TTAA 6 cut(s) 80, 104, 803, 1146, 1814, 2093
SatI GCNGC 8 cut(s) 15, 192, 223, 393, 606, 1823, 2060, 2130
Sau96I GGNCC 5 cut(s) 308, 1304, 1316, 1369, 1402
SchI GAGTC 1 cut(s) 1028
ScrFI CCNGG 5 cut(s) 383, 468, 1139, 1400, 1998
SduI GDGCHC 5 cut(s) 159, 283, 650, 712, 843
SfcI CTRYAG 4 cut(s) 393, 955, 1096, 1842
SfuI TTCGAA 1 cut(s) 1243
SinI GGWCC 3 cut(s) 308, 1304, 1316
SmiMI CAYNNNNRTG 5 cut(s) 303, 1210, 1428, 1731, 2013
SmlI CTYRAG 3 cut(s) 121, 527, 1415
SmoI CTYRAG 3 cut(s) 121, 527, 1415
SpeI ACTAGT 2 cut(s) 697, 1118
SsiI CCGC 7 cut(s) 15, 594, 929, 1222, 1601, 1847, 2102
SspI AATATT 2 cut(s) 1720, 1762
SspMI CTAG 9 cut(s) 302, 389, 698, 1119, 1227, 1449, 1545, 1934, 1949
SstI GAGCTC 2 cut(s) 650, 843
StyD4I CCNGG 5 cut(s) 381, 466, 1137, 1398, 1996
StyI CCWWGG 5 cut(s) 301, 356, 732, 1509, 1564
TaaI ACNGT 7 cut(s) 154, 441, 538, 1325, 1343, 1879, 2281
TaiI ACGT 5 cut(s) 36, 405, 499, 906, 1790
TaqI TCGA 8 cut(s) 137, 559, 633, 656, 892, 1243, 1426, 1581
TaqII GACCGA 1 cut(s) 1668
TatI WGTACW 5 cut(s) 547, 576, 864, 1611, 1836
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 3 cut(s) 58, 1256, 1851
Tru1I TTAA 6 cut(s) 80, 104, 803, 1146, 1814, 2093
Tru9I TTAA 6 cut(s) 80, 104, 803, 1146, 1814, 2093
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 3 cut(s) 1114, 1772, 2188
TseI GCWGC 7 cut(s) 191, 222, 392, 605, 1822, 2059, 2129
Tsp45I GTSAC 3 cut(s) 1114, 1772, 2188
TspDTI ATGAA 9 cut(s) 255, 813, 870, 913, 1145, 1267, 1471, 1517, 1631
TspGWI ACGGA 1 cut(s) 1249
TspRI CASTG 1 cut(s) 402
Tth111I GACNNNGTC 1 cut(s) 1789
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 3 cut(s) 308, 1304, 1316
VspI ATTAAT 1 cut(s) 2093
XapI RAATTY 2 cut(s) 1477, 1895
XceI RCATGY 1 cut(s) 2046
XcmI CCANNNNNNNNNTGG 1 cut(s) 877
XmaJI CCTAGG 1 cut(s) 301
XspI CTAG 9 cut(s) 302, 389, 698, 1119, 1227, 1449, 1545, 1934, 1949
ZraI GACGTC 1 cut(s) 34
Zsp2I ATGCAT 1 cut(s) 2044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.