Rmu_sc0002351.1_g000026

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002351.1
OG0000662
Physical Location & Seq
Reverse (-)
78318 .. 80269
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002351.1_g000026.1.cds

Sequence Viewer

Length: 1062 bp
atggcttcatcagcggctcctatttctgacagcctcaagaagatctgggatacgtggaatctccggagcttcatcatgttaagtctcacattgcagaccttcataattctctttgcacccttgagaaagcgatcatcgagcaaatgggtaatcgttcccatctggttggcttacttgctcgctgacggggctgctaacttcgctatcgaacaaattgcaagcagccaaatgaacactgatcaaggttcgccagagaatggtaatctattggcattctgggctccctttcttcttttgcaccttggtggtccaaacaccataattgcaattgctatggaagacaatgcgttgtggccggccaattcacttgcttggtctcatattccaggttgtagctgcaatgtacgtctgcgaattttcatagagaaacatggctttcacaggtttgatgttggaattacttattccttgctttttggtgccatagctcttgattcaatagctctttcgatgctgatattatctgattggacaatcgctgttgtatacaagtactgtgaaaagtgttgtgtagtcattgatcacatgatgaagtacctggagtttaagagatcatggcgaccaactacgccaccaaagtatctggagcggaccagaaagattttatttcgaagatggtgtgaatctctctccggtttcagcttcatagattattctctccgagaatgtggaagatcagtagtccgacctgcaattttggaccaaattggcatttaccgaaggatcatcagatttttgggctacctttaccatagaatcatcatatttctaggccacctttaccccagaatcattaaattctttggccttaagtatcttcgagatgaaatgaagtacagatccagcaagagactggaagagaatttgtggaaattcatttttgatgagttgaaaagtaaatcctcagaagttgatgatcccaaaattggtagagaaatatgttcgtcccaaggggattgggttctcctaaaaaagtatgagaagcatccggaataccattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

353

Amino Acids

41.07

Weight (kDa)

9.33

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 757
AccB1I GGYRCC 1 cut(s) 479
AccB7I CCANNNNNTGG 1 cut(s) 257
AccBSI CCGCTC 1 cut(s) 649
AccI GTMKAC 1 cut(s) 546
AccIII TCCGGA 2 cut(s) 63, 1048
AciI CCGC 2 cut(s) 14, 649
AclWI GGATC 3 cut(s) 791, 894, 971
AcoI YGGCCR 2 cut(s) 353, 357
AcsI RAATTY 4 cut(s) 414, 857, 922, 932
AfaI GTAC 4 cut(s) 405, 554, 596, 896
AfiI CCNNNNNNNGG 2 cut(s) 257, 986
AflII CTTAAG 1 cut(s) 869
AgsI TTSAA 2 cut(s) 498, 952
AjnI CCWGG 2 cut(s) 385, 597
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 5 cut(s) 69, 396, 488, 503, 702
AluI AGCT 5 cut(s) 69, 396, 488, 503, 702
Alw26I GTCTC 3 cut(s) 89, 381, 904
AlwI GGATC 3 cut(s) 791, 894, 971
Aor13HI TCCGGA 2 cut(s) 63, 1048
AoxI GGCC 4 cut(s) 353, 357, 832, 865
ApeKI GCWGC 3 cut(s) 191, 222, 396
ApoI RAATTY 4 cut(s) 414, 857, 922, 932
AspS9I GGNCC 3 cut(s) 308, 651, 760
AsuII TTCGAA 1 cut(s) 670
AvaII GGWCC 3 cut(s) 308, 651, 760
BaeI ACNNNNGTAYC 2 cut(s) 623, 656
BanI GGYRCC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 345
BbvI GCAGC 3 cut(s) 178, 234, 383
BccI CCATC 2 cut(s) 167, 669
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 2 cut(s) 387, 599
BciVI GTATCC 1 cut(s) 43
BclI TGATCA 2 cut(s) 238, 580
BcoDI GTCTC 3 cut(s) 89, 381, 904
BfaI CTAG 1 cut(s) 830
BfrI CTTAAG 1 cut(s) 869
BfuAI ACCTGC 1 cut(s) 757
BfuI GTATCC 1 cut(s) 43
BglII AGATCT 1 cut(s) 42
BisI GCNGC 4 cut(s) 15, 192, 223, 397
BlsI GCNGC 4 cut(s) 16, 193, 224, 398
BmcAI AGTACT 1 cut(s) 554
Bme1390I CCNGG 2 cut(s) 387, 599
Bme18I GGWCC 3 cut(s) 308, 651, 760
BmgT120I GGNCC 3 cut(s) 308, 651, 760
BmiI GGNNCC 3 cut(s) 18, 282, 481
BmrFI CCNGG 2 cut(s) 387, 599
BmsI GCATC 2 cut(s) 501, 1054
BpiI GAAGAC 1 cut(s) 345
BpmI CTGGAG 2 cut(s) 620, 665
Bpu14I TTCGAA 1 cut(s) 670
BpuEI CTTGAG 2 cut(s) 20, 142
BsaAI YACGTR 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 381
BsaJI CCNNGG 2 cut(s) 301, 1009
BsaWI WCCGGW 3 cut(s) 63, 692, 1048
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 986
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 1 cut(s) 918
Bse3DI GCAATG 2 cut(s) 89, 406
BseAI TCCGGA 2 cut(s) 63, 1048
BseBI CCWGG 2 cut(s) 387, 599
BseDI CCNNGG 2 cut(s) 301, 1009
BseGI GGATG 1 cut(s) 1045
BseLI CCNNNNNNNGG 2 cut(s) 257, 986
BseMI GCAATG 2 cut(s) 89, 406
BseMII CTCAG 1 cut(s) 978
BseNI ACTGG 1 cut(s) 918
BseXI GCAGC 3 cut(s) 178, 234, 383
BshFI GGCC 4 cut(s) 355, 359, 834, 867
BshNI GGYRCC 1 cut(s) 479
BsiSI CCGG 4 cut(s) 64, 356, 693, 1049
BslFI GGGAC 1 cut(s) 991
BslI CCNNNNNNNGG 2 cut(s) 257, 986
BsmAI GTCTC 3 cut(s) 89, 381, 904
BsmFI GGGAC 1 cut(s) 991
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 4 cut(s) 355, 359, 834, 867
Bso31I GGTCTC 1 cut(s) 381
Bsp119I TTCGAA 1 cut(s) 670
Bsp1286I GDGCHC 1 cut(s) 283
Bsp13I TCCGGA 2 cut(s) 63, 1048
Bsp143I GATC 9 cut(s) 42, 131, 238, 580, 611, 734, 783, 899, 976
BspACI CCGC 2 cut(s) 14, 649
BspANI GGCC 4 cut(s) 355, 359, 834, 867
BspCNI CTCAG 1 cut(s) 977
BspEI TCCGGA 2 cut(s) 63, 1048
BspLI GGNNCC 3 cut(s) 18, 282, 481
BspMI ACCTGC 1 cut(s) 757
BspPI GGATC 3 cut(s) 791, 894, 971
BspT104I TTCGAA 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 479
BspTI CTTAAG 1 cut(s) 869
BspTNI GGTCTC 1 cut(s) 381
BsrBI CCGCTC 1 cut(s) 649
BsrDI GCAATG 2 cut(s) 89, 406
BsrFI RCCGGY 1 cut(s) 355
BsrI ACTGG 1 cut(s) 918
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 2 cut(s) 301, 1009
BssMI GATC 9 cut(s) 42, 131, 238, 580, 611, 734, 783, 899, 976
BssNAI GTATAC 1 cut(s) 547
BssT1I CCWWGG 2 cut(s) 301, 1009
Bst1107I GTATAC 1 cut(s) 547
Bst2UI CCWGG 2 cut(s) 387, 599
Bst4CI ACNGT 1 cut(s) 557
Bst6I CTCTTC 1 cut(s) 912
BstAFI CTTAAG 1 cut(s) 869
BstBAI YACGTR 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 670
BstC8I GCNNGC 3 cut(s) 180, 220, 357
BstDEI CTNAG 1 cut(s) 964
BstF5I GGATG 1 cut(s) 1045
BstKTI GATC 9 cut(s) 45, 134, 241, 583, 614, 737, 786, 902, 979
BstMAI GTCTC 3 cut(s) 89, 381, 904
BstMBI GATC 9 cut(s) 42, 131, 238, 580, 611, 734, 783, 899, 976
BstMWI GCNNNNNNNGC 4 cut(s) 11, 188, 200, 278
BstNI CCWGG 2 cut(s) 387, 599
BstSCI CCNGG 2 cut(s) 385, 597
BstV1I GCAGC 3 cut(s) 178, 234, 383
BstV2I GAAGAC 1 cut(s) 345
BstX2I RGATCY 2 cut(s) 42, 899
BstXI CCANNNNNNTGG 1 cut(s) 166
BstYI RGATCY 2 cut(s) 42, 899
BstZ17I GTATAC 1 cut(s) 547
BsuI GTATCC 1 cut(s) 43
BsuRI GGCC 4 cut(s) 355, 359, 834, 867
BtsCI GGATG 1 cut(s) 1045
BtsIMutI CAGTG 1 cut(s) 234
BveI ACCTGC 1 cut(s) 757
Cac8I GCNNGC 3 cut(s) 180, 220, 357
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 3 cut(s) 308, 651, 760
Csp6I GTAC 4 cut(s) 404, 553, 595, 895
CviAII CATG 4 cut(s) 76, 431, 586, 615
CviQI GTAC 4 cut(s) 404, 553, 595, 895
DdeI CTNAG 1 cut(s) 964
DpnI GATC 9 cut(s) 44, 133, 240, 582, 613, 736, 785, 901, 978
DpnII GATC 9 cut(s) 42, 131, 238, 580, 611, 734, 783, 899, 976
EaeI YGGCCR 2 cut(s) 353, 357
Eam1104I CTCTTC 1 cut(s) 912
EarI CTCTTC 1 cut(s) 912
Eco130I CCWWGG 2 cut(s) 301, 1009
Eco24I GRGCYC 1 cut(s) 283
Eco31I GGTCTC 1 cut(s) 381
Eco47I GGWCC 3 cut(s) 308, 651, 760
EcoRII CCWGG 2 cut(s) 385, 597
EcoT14I CCWWGG 2 cut(s) 301, 1009
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 2 cut(s) 301, 1009
FaeI CATG 4 cut(s) 79, 434, 589, 618
FaqI GGGAC 1 cut(s) 991
FatI CATG 4 cut(s) 75, 430, 585, 614
FbaI TGATCA 2 cut(s) 238, 580
FblI GTMKAC 1 cut(s) 546
Fnu4HI GCNGC 4 cut(s) 15, 192, 223, 397
FokI GGATG 1 cut(s) 1032
FriOI GRGCYC 1 cut(s) 283
FseI GGCCGGCC 1 cut(s) 359
Fsp4HI GCNGC 4 cut(s) 15, 192, 223, 397
FspBI CTAG 1 cut(s) 830
GluI GCNGC 4 cut(s) 15, 192, 223, 397
GsuI CTGGAG 2 cut(s) 620, 665
HaeIII GGCC 4 cut(s) 355, 359, 834, 867
HapII CCGG 4 cut(s) 64, 356, 693, 1049
Hin1II CATG 4 cut(s) 79, 434, 589, 618
HinfI GANTC 5 cut(s) 58, 494, 683, 816, 849
HpaII CCGG 4 cut(s) 64, 356, 693, 1049
Hpy166II GTNNAC 1 cut(s) 547
Hpy188I TCNGA 6 cut(s) 28, 526, 722, 746, 791, 967
Hpy188III TCNNGA 6 cut(s) 37, 64, 491, 644, 881, 1049
Hpy8I GTNNAC 1 cut(s) 547
HpyAV CCTTC 2 cut(s) 109, 774
HpyCH4III ACNGT 1 cut(s) 557
HpyCH4IV ACGT 2 cut(s) 53, 406
HpyCH4V TGCA 7 cut(s) 94, 116, 218, 298, 326, 399, 752
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 188, 200, 278
HpyF3I CTNAG 1 cut(s) 964
HpySE526I ACGT 2 cut(s) 53, 406
Hsp92II CATG 4 cut(s) 79, 434, 589, 618
Kpn2I TCCGGA 2 cut(s) 63, 1048
KroI GCCGGC 1 cut(s) 355
KroNI GCCGGC 1 cut(s) 357
Ksp22I TGATCA 2 cut(s) 238, 580
Kzo9I GATC 9 cut(s) 42, 131, 238, 580, 611, 734, 783, 899, 976
LmnI GCTCC 4 cut(s) 22, 66, 286, 646
Lsp1109I GCAGC 3 cut(s) 178, 234, 383
LweI GCATC 2 cut(s) 501, 1054
MaeI CTAG 1 cut(s) 830
MaeII ACGT 2 cut(s) 53, 406
MalI GATC 9 cut(s) 44, 133, 240, 582, 613, 736, 785, 901, 978
MbiI CCGCTC 1 cut(s) 649
MboI GATC 9 cut(s) 42, 131, 238, 580, 611, 734, 783, 899, 976
MboII GAAGA 7 cut(s) 52, 281, 350, 684, 744, 869, 929
MfeI CAATTG 1 cut(s) 327
MflI RGATCY 2 cut(s) 42, 899
MhlI GDGCHC 1 cut(s) 283
MmeI TCCRAC 2 cut(s) 433, 769
MnlI CCTC 2 cut(s) 44, 973
MroI TCCGGA 2 cut(s) 63, 1048
MroNI GCCGGC 1 cut(s) 355
MseI TTAA 5 cut(s) 80, 606, 855, 870, 1060
MslI CAYNNNNRTG 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 14
MspCI CTTAAG 1 cut(s) 869
MspI CCGG 4 cut(s) 64, 356, 693, 1049
MspR9I CCNGG 2 cut(s) 387, 599
MunI CAATTG 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 272
MvaI CCWGG 2 cut(s) 387, 599
MwoI GCNNNNNNNGC 4 cut(s) 11, 188, 200, 278
NaeI GCCGGC 1 cut(s) 357
NdeII GATC 9 cut(s) 42, 131, 238, 580, 611, 734, 783, 899, 976
NgoMIV GCCGGC 1 cut(s) 355
NlaIII CATG 4 cut(s) 79, 434, 589, 618
NlaIV GGNNCC 3 cut(s) 18, 282, 481
NspV TTCGAA 1 cut(s) 670
OliI CACNNNNGTG 1 cut(s) 303
PctI GAATGC 1 cut(s) 272
PdiI GCCGGC 1 cut(s) 357
PfeI GAWTC 5 cut(s) 58, 494, 683, 816, 849
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 4 cut(s) 16, 193, 224, 398
Ppu21I YACGTR 1 cut(s) 54
Psp6I CCWGG 2 cut(s) 385, 597
PspGI CCWGG 2 cut(s) 385, 597
PspN4I GGNNCC 3 cut(s) 18, 282, 481
PspPI GGNCC 3 cut(s) 308, 651, 760
PsuI RGATCY 2 cut(s) 42, 899
RigI GGCCGGCC 1 cut(s) 359
RsaI GTAC 4 cut(s) 405, 554, 596, 896
RsaNI GTAC 4 cut(s) 404, 553, 595, 895
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 5 cut(s) 80, 606, 855, 870, 1060
SatI GCNGC 4 cut(s) 15, 192, 223, 397
Sau3AI GATC 9 cut(s) 42, 131, 238, 580, 611, 734, 783, 899, 976
Sau96I GGNCC 3 cut(s) 308, 651, 760
ScaI AGTACT 1 cut(s) 554
ScrFI CCNGG 2 cut(s) 387, 599
SduI GDGCHC 1 cut(s) 283
SfaNI GCATC 2 cut(s) 501, 1054
SfuI TTCGAA 1 cut(s) 670
SinI GGWCC 3 cut(s) 308, 651, 760
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 3 cut(s) 35, 121, 869
SmoI CTYRAG 3 cut(s) 35, 121, 869
SsiI CCGC 2 cut(s) 14, 649
SspMI CTAG 1 cut(s) 830
StyD4I CCNGG 2 cut(s) 385, 597
StyI CCWWGG 2 cut(s) 301, 1009
TaaI ACNGT 1 cut(s) 557
TaiI ACGT 2 cut(s) 56, 409
TaqI TCGA 5 cut(s) 137, 207, 509, 670, 880
TatI WGTACW 2 cut(s) 552, 894
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 5 cut(s) 58, 494, 683, 816, 849
Tru1I TTAA 5 cut(s) 80, 606, 855, 870, 1060
Tru9I TTAA 5 cut(s) 80, 606, 855, 870, 1060
TscAI CASTG 1 cut(s) 241
TseI GCWGC 3 cut(s) 191, 222, 396
TspDTI ATGAA 9 cut(s) 61, 91, 245, 409, 605, 694, 900, 905, 925
TspRI CASTG 1 cut(s) 241
Van91I CCANNNNNTGG 1 cut(s) 257
Vha464I CTTAAG 1 cut(s) 869
VpaK11BI GGWCC 3 cut(s) 308, 651, 760
XapI RAATTY 4 cut(s) 414, 857, 922, 932
XcmI CCANNNNNNNNNTGG 1 cut(s) 910
XmiI GTMKAC 1 cut(s) 546
XspI CTAG 1 cut(s) 830
ZrmI AGTACT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.