MD17G1216700.v1.1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000662
Physical Location & Seq
Reverse (-)
26348539 .. 26350602
2064 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1216700.v1.1.491

Sequence Viewer

Length: 2064 bp
ATGCAAAAGAGATTCAAATTTCTTAAAAGAGAATGGGAATCTCTCCCCTGCCTGCCAATATATATTGTAGAAACTAACTTTAAAAATTCTCTCCAGGGAAAGGAAATGAGCCAAAGAAAGTATATGGATTCCCCAACGGATTCCCCAATTCCTAGCAGCGTCATGAAGATATGGGATCAGTGGAATCTCCAGGGCATTATCATCTTAAGCCTCTCATTACAGACCTTTTTAATTCTTTTTGCGCCCTTGAGAAAGCGCACGTCCAACAAATTGATGCTCATGCCTATTTGGTTAGCTTACTTGCTCGCTGACTGGGCTGCTAGCTGTGTAGTTGGATTAATTTCGAGCAGACAAAGCGATACTCAGAACCATGGAGATTATGGTGATCTTCTGGCATTTTGGGCTCCCTTTCTTTTGTTACACCTTGGCGGTCCAGACACTATAATTGCCATTGCTCTTGAAGATAATGCCTTGTGGCCAAGGCACTTGCTAGGGCTCTTATTCCAAGCTATAGCTGCACTTTACATCTTCGTTCAATCATTACCGCGGAACAAACTATGGATCCCAACTCTCATCTTGTTTCTTGCAGGAATCATTAAGTATGTAGAACGCACACGCGCTTTGTATCTTGCAAGCGTGGATGGTCTCAAGGAAGTCATGCTAAGAAAACCTGAACCTGGACGGGACTATGCAAAGTTCATGGATGAGTACTCGTCAAAAAAGGATGCCAACCTTCCAATTGAAGTCCATGTCACAGAACACTCCATAATGGATAAACCTAATGTTTATCGCCTAGGACGAGACGAAATGGACGATAATGTTGTAGTAGTGCGACACGCTTATCACTTCTTTCAAATCTTCAAGGCTCTTATTATAGGCCTTAAGCTTAGTTTTTATGAACGCAAAGAGAGCCGTGCGATTTTCCTTGAGAGAACCTCACAAGAAGCTCTTGACTTAGTTACTACTGAACTCAACTTTATGTATGAATCTTTCTACACAAAGGTGGCTGTGGTGCATTCTAAGCTAGGATACATTTTCCGTGCTATATCCTTCTGCGCAGTTTCTGTTGCCCTTTTTTTCTTCTATAAATTAGAGAAACACGACTTCCAGAGGTTTGATATTGTAATTACTTATACCTTGCTTTATGGTGCCATAGGACTGGATTTAATGGCTGTTTTCATCCTCGTATTCTCTGATTGGACTGCCATTACTATGAGTAACTCTTGGAGAGATTCTGTTTTGGGTACTATCTTAGGTGCATATCTTAACTTCAAGAGAACAAGGTGTCTTAAGAAAGCAACCAGTTGTTTGGAGCGGACCATGCGATTTCTATTTCGAAGATGGTCTGAATCTGTCTCTACATTCAACTTGATCGATTATTCGCTCAAGGAATGTACAAAAGTGAGACCTACAGTTATCGACTTATTGGGCCTCAAGGGTCTTCGAGAGAAGATGAAATATGTGTCCCACAAGCCATTCACTATGGGGTTATGGGATTTCATATTTGAAGAGTTGAAATATAAATCTATACTCGCTAAAGACCCTCAAGCTTCCAAAAAATTATTTTTAACCAGAGGTGATTGGATTCTCCAAGATAACGAATTGTGTGGTGAACGCAATGAGTTACTACCGTTTGTTGATGATTTTAACTATGCTCAGAGCCTTCTGTTGTGGCACATTGCTACAGATCTATGCGATACCCTTGACAAGAGCAATGCAGAGGATCGTGGCTGTCGTGAATACAAAGCAGATGATCATGGCTGCCGTGAATATAGTAAGACCATGTCTGGTTACATGCTATACCTTTTAGTTATGCAGACTACTATGATGTCGTCTGTAGCAGGAGTTGCGCAAATCGAATTGTTGGATACGTGTGCAGAAGCCAAAAGTTTCTTTAGCAAAAGGAAACTAGGACCAGGAAAACATGAAGATGCCTGTAATAGATTCCTCAAGGTGAATACAGAAGTTAAACCTGTATTTGTGAGAGGGCGAAGAAGTAAATCTGTATGGTTTGATGCATGCATTCTGGCCAAAGAGTTGATGAAATTGGAAGAAAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

688

Amino Acids

79.57

Weight (kDa)

8.92

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 97 - 460 7.7e-97 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1057, 1851
AccB1I GGYRCC 1 cut(s) 1148
AccBSI CCGCTC 1 cut(s) 1315
AccII CGCG 2 cut(s) 547, 618
AciI CCGC 4 cut(s) 429, 545, 547, 1315
AclWI GGATC 4 cut(s) 183, 556, 569, 1731
AcoI YGGCCR 2 cut(s) 476, 2028
AcsI RAATTY 2 cut(s) 17, 85
AfaI GTAC 3 cut(s) 710, 1246, 1396
AfiI CCNNNNNNNGG 2 cut(s) 100, 677
AflII CTTAAG 3 cut(s) 205, 881, 1289
AflIII ACRYGT 1 cut(s) 1871
AjiI CACGTC 1 cut(s) 261
AjnI CCWGG 4 cut(s) 93, 189, 676, 1915
AleI CACNNNNGTG 1 cut(s) 1001
AluBI AGCT 8 cut(s) 296, 324, 509, 515, 886, 947, 1024, 1550
AluI AGCT 8 cut(s) 296, 324, 509, 515, 886, 947, 1024, 1550
Alw26I GTCTC 4 cut(s) 650, 795, 1360, 1399
AlwI GGATC 4 cut(s) 183, 556, 569, 1731
AlwNI CAGNNNCTG 1 cut(s) 1064
AoxI GGCC 4 cut(s) 476, 877, 1429, 2028
ApeKI GCWGC 4 cut(s) 156, 317, 515, 1761
ApoI RAATTY 2 cut(s) 17, 85
AseI ATTAAT 1 cut(s) 338
AspA2I CCTAGG 1 cut(s) 793
AspLEI GCGC 5 cut(s) 244, 258, 620, 1058, 1852
AspS9I GGNCC 4 cut(s) 431, 1317, 1429, 1913
AsuHPI GGTGA 4 cut(s) 395, 1589, 1622, 1966
AsuII TTCGAA 1 cut(s) 1336
AsuNHI GCTAGC 1 cut(s) 320
AvaII GGWCC 3 cut(s) 431, 1317, 1913
AvrII CCTAGG 1 cut(s) 793
BalI TGGCCA 2 cut(s) 478, 2030
BamHI GGATCC 1 cut(s) 561
BanI GGYRCC 1 cut(s) 1148
BanII GRGCYC 2 cut(s) 406, 498
BbsI GAAGAC 1 cut(s) 1433
BbvI GCAGC 4 cut(s) 168, 304, 502, 1748
BccI CCATC 2 cut(s) 635, 1335
BceAI ACGGC 2 cut(s) 897, 1749
BciT130I CCWGG 4 cut(s) 95, 191, 678, 1917
BciVI GTATCC 2 cut(s) 1022, 1861
BclI TGATCA 1 cut(s) 1753
BcoDI GTCTC 4 cut(s) 650, 795, 1360, 1399
BfaI CTAG 6 cut(s) 153, 321, 491, 794, 1025, 1910
BfmI CTRYAG 4 cut(s) 510, 1410, 1683, 1836
BfrI CTTAAG 3 cut(s) 205, 881, 1289
BfuI GTATCC 2 cut(s) 1022, 1861
BglII AGATCT 1 cut(s) 1687
BisI GCNGC 4 cut(s) 157, 318, 516, 1762
BlnI CCTAGG 1 cut(s) 793
BlsI GCNGC 4 cut(s) 158, 319, 517, 1763
BmcAI AGTACT 1 cut(s) 710
Bme1390I CCNGG 4 cut(s) 95, 191, 678, 1917
Bme18I GGWCC 3 cut(s) 431, 1317, 1913
BmgBI CACGTC 1 cut(s) 261
BmgT120I GGNCC 4 cut(s) 431, 1317, 1429, 1913
BmiI GGNNCC 3 cut(s) 405, 563, 1150
BmrFI CCNGG 4 cut(s) 95, 191, 678, 1917
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 4 cut(s) 264, 715, 1921, 2005
BmtI GCTAGC 1 cut(s) 324
BmuI ACTGGG 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 1433
BplI GAGNNNNNCTC 2 cut(s) 920, 952
BpmI CTGGAG 2 cut(s) 77, 173
Bpu14I TTCGAA 1 cut(s) 1336
BpuEI CTTGAG 7 cut(s) 268, 632, 947, 1370, 1418, 1530, 1934
Bsa29I ATCGAT 1 cut(s) 1374
BsaAI YACGTR 1 cut(s) 1872
BsaI GGTCTC 2 cut(s) 650, 1399
BsaJI CCNNGG 7 cut(s) 94, 190, 370, 424, 479, 545, 793
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 677
Bse1I ACTGG 3 cut(s) 317, 1164, 1302
Bse3DI GCAATG 4 cut(s) 450, 1624, 1677, 1720
BseBI CCWGG 4 cut(s) 95, 191, 678, 1917
BseCI ATCGAT 1 cut(s) 1374
BseDI CCNNGG 7 cut(s) 94, 190, 370, 424, 479, 545, 793
BseGI GGATG 4 cut(s) 646, 709, 730, 1179
BseLI CCNNNNNNNGG 2 cut(s) 100, 677
BseMI GCAATG 4 cut(s) 450, 1624, 1677, 1720
BseMII CTCAG 2 cut(s) 377, 1670
BseNI ACTGG 3 cut(s) 317, 1164, 1302
BseXI GCAGC 4 cut(s) 168, 304, 502, 1748
BsgI GTGCAG 2 cut(s) 501, 1896
Bsh1236I CGCG 2 cut(s) 547, 618
BshFI GGCC 4 cut(s) 478, 879, 1431, 2030
BshNI GGYRCC 1 cut(s) 1148
BshVI ATCGAT 1 cut(s) 1374
BslFI GGGAC 2 cut(s) 698, 1450
BslI CCNNNNNNNGG 2 cut(s) 100, 677
BsmAI GTCTC 4 cut(s) 650, 795, 1360, 1399
BsmBI CGTCTC 1 cut(s) 795
BsmFI GGGAC 2 cut(s) 698, 1450
BsmI GAATGC 2 cut(s) 1015, 2022
BsnI GGCC 4 cut(s) 478, 879, 1431, 2030
Bso31I GGTCTC 2 cut(s) 650, 1399
Bsp119I TTCGAA 1 cut(s) 1336
Bsp1286I GDGCHC 2 cut(s) 406, 498
Bsp1407I TGTACA 1 cut(s) 1394
Bsp143I GATC 7 cut(s) 175, 385, 561, 1371, 1687, 1723, 1753
Bsp19I CCATGG 1 cut(s) 370
BspACI CCGC 4 cut(s) 429, 545, 547, 1315
BspANI GGCC 4 cut(s) 478, 879, 1431, 2030
BspCNI CTCAG 2 cut(s) 376, 1669
BspDI ATCGAT 1 cut(s) 1374
BspFNI CGCG 2 cut(s) 547, 618
BspHI TCATGA 1 cut(s) 162
BspLI GGNNCC 3 cut(s) 405, 563, 1150
BspOI GCTAGC 1 cut(s) 324
BspPI GGATC 4 cut(s) 183, 556, 569, 1731
BspT104I TTCGAA 1 cut(s) 1336
BspT107I GGYRCC 1 cut(s) 1148
BspTI CTTAAG 3 cut(s) 205, 881, 1289
BspTNI GGTCTC 2 cut(s) 650, 1399
BsrBI CCGCTC 1 cut(s) 1315
BsrDI GCAATG 4 cut(s) 450, 1624, 1677, 1720
BsrGI TGTACA 1 cut(s) 1394
BsrI ACTGG 3 cut(s) 317, 1164, 1302
BssECI CCNNGG 7 cut(s) 94, 190, 370, 424, 479, 545, 793
BssMI GATC 7 cut(s) 175, 385, 561, 1371, 1687, 1723, 1753
BssT1I CCWWGG 4 cut(s) 370, 424, 479, 793
Bst2UI CCWGG 4 cut(s) 95, 191, 678, 1917
Bst4CI ACNGT 2 cut(s) 1414, 1632
Bst6I CTCTTC 1 cut(s) 1503
BstAFI CTTAAG 3 cut(s) 205, 881, 1289
BstAPI GCANNNNNTGC 1 cut(s) 1847
BstAUI TGTACA 1 cut(s) 1394
BstBAI YACGTR 1 cut(s) 1872
BstBI TTCGAA 1 cut(s) 1336
BstC8I GCNNGC 5 cut(s) 53, 306, 322, 634, 2020
BstDEI CTNAG 7 cut(s) 363, 662, 887, 955, 1020, 1252, 1656
BstDSI CCRYGG 2 cut(s) 370, 545
BstF5I GGATG 4 cut(s) 646, 709, 730, 1179
BstFNI CGCG 2 cut(s) 547, 618
BstHHI GCGC 5 cut(s) 244, 258, 620, 1058, 1852
BstKTI GATC 7 cut(s) 178, 388, 564, 1374, 1690, 1726, 1756
BstMAI GTCTC 4 cut(s) 650, 795, 1360, 1399
BstMBI GATC 7 cut(s) 175, 385, 561, 1371, 1687, 1723, 1753
BstMWI GCNNNNNNNGC 8 cut(s) 314, 354, 401, 515, 909, 1021, 1321, 1847
BstNI CCWGG 4 cut(s) 95, 191, 678, 1917
BstNSI RCATGY 2 cut(s) 1798, 2022
BstSCI CCNGG 4 cut(s) 93, 189, 676, 1915
BstSFI CTRYAG 4 cut(s) 510, 1410, 1683, 1836
BstUI CGCG 2 cut(s) 547, 618
BstV1I GCAGC 4 cut(s) 168, 304, 502, 1748
BstV2I GAAGAC 1 cut(s) 1433
BstX2I RGATCY 2 cut(s) 561, 1687
BstXI CCANNNNNNTGG 2 cut(s) 1159, 1309
BstYI RGATCY 2 cut(s) 561, 1687
Bsu15I ATCGAT 1 cut(s) 1374
BsuI GTATCC 2 cut(s) 1022, 1861
BsuRI GGCC 4 cut(s) 478, 879, 1431, 2030
BsuTUI ATCGAT 1 cut(s) 1374
BtgI CCRYGG 2 cut(s) 370, 545
BtrI CACGTC 1 cut(s) 261
BtsCI GGATG 4 cut(s) 646, 709, 730, 1179
BtsIMutI CAGTG 1 cut(s) 185
Cac8I GCNNGC 5 cut(s) 53, 306, 322, 634, 2020
CaiI CAGNNNCTG 1 cut(s) 1064
CciI TCATGA 1 cut(s) 162
CfoI GCGC 5 cut(s) 244, 258, 620, 1058, 1852
Cfr13I GGNCC 4 cut(s) 431, 1317, 1429, 1913
Cfr42I CCGCGG 1 cut(s) 548
ClaI ATCGAT 1 cut(s) 1374
CseI GACGC 1 cut(s) 148
Csp6I GTAC 3 cut(s) 709, 1245, 1395
CviQI GTAC 3 cut(s) 709, 1245, 1395
DdeI CTNAG 7 cut(s) 363, 662, 887, 955, 1020, 1252, 1656
DpnI GATC 7 cut(s) 177, 387, 563, 1373, 1689, 1725, 1755
DpnII GATC 7 cut(s) 175, 385, 561, 1371, 1687, 1723, 1753
DraI TTTAAA 1 cut(s) 82
EaeI YGGCCR 2 cut(s) 476, 2028
Eam1104I CTCTTC 1 cut(s) 1503
EarI CTCTTC 1 cut(s) 1503
Eco130I CCWWGG 4 cut(s) 370, 424, 479, 793
Eco147I AGGCCT 1 cut(s) 879
Eco24I GRGCYC 2 cut(s) 406, 498
Eco31I GGTCTC 2 cut(s) 650, 1399
Eco47I GGWCC 3 cut(s) 431, 1317, 1913
EcoRII CCWGG 4 cut(s) 93, 189, 676, 1915
EcoT14I CCWWGG 4 cut(s) 370, 424, 479, 793
EcoT22I ATGCAT 2 cut(s) 2020, 2024
EcoT38I GRGCYC 2 cut(s) 406, 498
ErhI CCWWGG 4 cut(s) 370, 424, 479, 793
Esp3I CGTCTC 1 cut(s) 795
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 2 cut(s) 698, 1450
FbaI TGATCA 1 cut(s) 1753
Fnu4HI GCNGC 4 cut(s) 157, 318, 516, 1762
FokI GGATG 4 cut(s) 653, 716, 737, 1166
FriOI GRGCYC 2 cut(s) 406, 498
Fsp4HI GCNGC 4 cut(s) 157, 318, 516, 1762
FspBI CTAG 6 cut(s) 153, 321, 491, 794, 1025, 1910
FspI TGCGCA 2 cut(s) 1057, 1851
GlaI GCGC 5 cut(s) 243, 257, 619, 1057, 1851
GluI GCNGC 4 cut(s) 157, 318, 516, 1762
GsuI CTGGAG 2 cut(s) 77, 173
HaeIII GGCC 4 cut(s) 478, 879, 1431, 2030
HgaI GACGC 1 cut(s) 148
HhaI GCGC 5 cut(s) 244, 258, 620, 1058, 1852
Hin6I GCGC 5 cut(s) 242, 256, 618, 1056, 1850
HinP1I GCGC 5 cut(s) 242, 256, 618, 1056, 1850
HindIII AAGCTT 2 cut(s) 884, 1548
HphI GGTGA 4 cut(s) 395, 1589, 1622, 1966
Hpy166II GTNNAC 1 cut(s) 1613
Hpy188I TCNGA 4 cut(s) 366, 1195, 1348, 1659
Hpy188III TCNNGA 8 cut(s) 163, 434, 458, 950, 1108, 1273, 1445, 1736
Hpy8I GTNNAC 1 cut(s) 1613
HpyAV CCTTC 3 cut(s) 743, 1060, 1673
HpyCH4III ACNGT 2 cut(s) 1414, 1632
HpyCH4IV ACGT 2 cut(s) 260, 1871
HpyF10VI GCNNNNNNNGC 8 cut(s) 314, 354, 401, 515, 909, 1021, 1321, 1847
HpyF3I CTNAG 7 cut(s) 363, 662, 887, 955, 1020, 1252, 1656
HpySE526I ACGT 2 cut(s) 260, 1871
HspAI GCGC 5 cut(s) 242, 256, 618, 1056, 1850
Ksp22I TGATCA 1 cut(s) 1753
KspI CCGCGG 1 cut(s) 548
Kzo9I GATC 7 cut(s) 175, 385, 561, 1371, 1687, 1723, 1753
LmnI GCTCC 2 cut(s) 409, 1312
Lsp1109I GCAGC 4 cut(s) 168, 304, 502, 1748
LweI GCATC 4 cut(s) 264, 715, 1921, 2005
MaeI CTAG 6 cut(s) 153, 321, 491, 794, 1025, 1910
MaeII ACGT 2 cut(s) 260, 1871
MaeIII GTNAC 6 cut(s) 417, 751, 958, 1217, 1623, 1790
MalI GATC 7 cut(s) 177, 387, 563, 1373, 1689, 1725, 1755
MbiI CCGCTC 1 cut(s) 1315
MboI GATC 7 cut(s) 175, 385, 561, 1371, 1687, 1723, 1753
MfeI CAATTG 1 cut(s) 738
MflI RGATCY 2 cut(s) 561, 1687
MhlI GDGCHC 2 cut(s) 406, 498
MlsI TGGCCA 2 cut(s) 478, 2030
MluNI TGGCCA 2 cut(s) 478, 2030
MmeI TCCRAC 3 cut(s) 288, 313, 1845
Mox20I TGGCCA 2 cut(s) 478, 2030
Mph1103I ATGCAT 2 cut(s) 2020, 2024
MscI TGGCCA 2 cut(s) 478, 2030
MslI CAYNNNNRTG 3 cut(s) 1001, 1211, 1929
Msp20I TGGCCA 2 cut(s) 478, 2030
MspA1I CMGCKG 1 cut(s) 547
MspCI CTTAAG 3 cut(s) 205, 881, 1289
MspR9I CCNGG 4 cut(s) 95, 191, 678, 1917
MunI CAATTG 1 cut(s) 738
Mva1269I GAATGC 2 cut(s) 1015, 2022
MvaI CCWGG 4 cut(s) 95, 191, 678, 1917
MvnI CGCG 2 cut(s) 547, 618
MwoI GCNNNNNNNGC 8 cut(s) 314, 354, 401, 515, 909, 1021, 1321, 1847
NcoI CCATGG 1 cut(s) 370
NdeII GATC 7 cut(s) 175, 385, 561, 1371, 1687, 1723, 1753
NheI GCTAGC 1 cut(s) 320
NlaIV GGNNCC 3 cut(s) 405, 563, 1150
NmuCI GTSAC 1 cut(s) 751
NsbI TGCGCA 2 cut(s) 1057, 1851
NsiI ATGCAT 2 cut(s) 2020, 2024
NspI RCATGY 2 cut(s) 1798, 2022
NspV TTCGAA 1 cut(s) 1336
OliI CACNNNNGTG 1 cut(s) 1001
PaeI GCATGC 1 cut(s) 2022
PagI TCATGA 1 cut(s) 162
PceI AGGCCT 1 cut(s) 879
PcsI WCGNNNNNNNCGW 3 cut(s) 796, 810, 1732
PctI GAATGC 2 cut(s) 1015, 2022
PflFI GACNNNGTC 1 cut(s) 1783
PkrI GCNGC 4 cut(s) 158, 319, 517, 1763
Ppu21I YACGTR 1 cut(s) 1872
PshBI ATTAAT 1 cut(s) 338
Psp6I CCWGG 4 cut(s) 93, 189, 676, 1915
PspGI CCWGG 4 cut(s) 93, 189, 676, 1915
PspN4I GGNNCC 3 cut(s) 405, 563, 1150
PspPI GGNCC 4 cut(s) 431, 1317, 1429, 1913
PstNI CAGNNNCTG 1 cut(s) 1064
PsuI RGATCY 2 cut(s) 561, 1687
PsyI GACNNNGTC 1 cut(s) 1783
RsaI GTAC 3 cut(s) 710, 1246, 1396
RsaNI GTAC 3 cut(s) 709, 1245, 1395
RseI CAYNNNNRTG 3 cut(s) 1001, 1211, 1929
SacII CCGCGG 1 cut(s) 548
SatI GCNGC 4 cut(s) 157, 318, 516, 1762
Sau3AI GATC 7 cut(s) 175, 385, 561, 1371, 1687, 1723, 1753
Sau96I GGNCC 4 cut(s) 431, 1317, 1429, 1913
ScaI AGTACT 1 cut(s) 710
ScrFI CCNGG 4 cut(s) 95, 191, 678, 1917
SduI GDGCHC 2 cut(s) 406, 498
SfaNI GCATC 4 cut(s) 264, 715, 1921, 2005
SfcI CTRYAG 4 cut(s) 510, 1410, 1683, 1836
Sfr303I CCGCGG 1 cut(s) 548
SfuI TTCGAA 1 cut(s) 1336
SgrBI CCGCGG 1 cut(s) 548
SinI GGWCC 3 cut(s) 431, 1317, 1913
SmiMI CAYNNNNRTG 3 cut(s) 1001, 1211, 1929
SphI GCATGC 1 cut(s) 2022
SseBI AGGCCT 1 cut(s) 879
SsiI CCGC 4 cut(s) 429, 545, 547, 1315
SspMI CTAG 6 cut(s) 153, 321, 491, 794, 1025, 1910
StuI AGGCCT 1 cut(s) 879
StyD4I CCNGG 4 cut(s) 93, 189, 676, 1915
StyI CCWWGG 4 cut(s) 370, 424, 479, 793
TaaI ACNGT 2 cut(s) 1414, 1632
TaiI ACGT 2 cut(s) 263, 1874
TaqI TCGA 6 cut(s) 344, 1336, 1374, 1419, 1444, 1857
TatI WGTACW 2 cut(s) 708, 1394
TscAI CASTG 1 cut(s) 185
TseFI GTSAC 1 cut(s) 751
TseI GCWGC 4 cut(s) 156, 317, 515, 1761
Tsp45I GTSAC 1 cut(s) 751
TspDTI ATGAA 9 cut(s) 179, 688, 912, 999, 1168, 1469, 1489, 1941, 2057
TspGWI ACGGA 2 cut(s) 152, 1028
TspRI CASTG 1 cut(s) 185
Tth111I GACNNNGTC 1 cut(s) 1783
Vha464I CTTAAG 3 cut(s) 205, 881, 1289
VpaK11BI GGWCC 3 cut(s) 431, 1317, 1913
VspI ATTAAT 1 cut(s) 338
XapI RAATTY 2 cut(s) 17, 85
XceI RCATGY 2 cut(s) 1798, 2022
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XmaJI CCTAGG 1 cut(s) 793
XspI CTAG 6 cut(s) 153, 321, 491, 794, 1025, 1910
ZrmI AGTACT 1 cut(s) 710
Zsp2I ATGCAT 2 cut(s) 2020, 2024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.