AT5G45470

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000662
Physical Location & Seq
Reverse (-)
18421985 .. 18425042
3058 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45470.1

Sequence Viewer

Length: 2601 bp
ATGGTGGAAGTGATACCAAAACATATCAAAGATGTTTGGGATAGGTGGAACATAAGAGGAGCAGTCATTCTAAGTCTTACCCTTCAAGCCATTCTCATTTGCTTCTCTCCTCTAAGGAAACGTACACCAAGAAGGCTCTTGATCGTGCTTGTTTGGTCCTCTTATCTCTTAGCAGATTGGTCTGCTAATTTCGCTGTTGGTCTTATCTCCAAGAATCAAGGTAAAGATCTCAAACCTGATGACCCTCCACAGGACAAAAAGGTAATGGCTTTATGGGCACCATTCTTGCTCTTGCATCTTGGTGGACCAGACACTATCACAGCTTTCGCGCTTGAAGATAACGCTCTCTGGCTACGACATGTCTTTGGGCTTGTCTTTCAGGCAATTGCTGGTGTCTATGTGGTTGTCATGTCTCTGCCCAATAGCCTTTGGGTGGTTATAGTCTTAGTGTTTGTTTCTGGGACTATAAAGTATCTGGAGAGGACAACGGCTCTGTATAGTGCAAGTTTGGACAAGTTTAGGGACTCTATGATTCAAGCACCAGATCCAGGACCAAACTACGCCAAGCTCATGGAGGAGTACAAGGCCAAGAAAGAGGCAAGACTTCCCACCAAGATCGTTCTGATTGATGAGCCTGACAAAGAAAATAGGCCTAAGAAGTTAGAGCATCCAGCATTAGCTTCGAAGAAAAGAAAAAAAGATTTGACCGATCTTGAGATTGTTCAGTATGCTTACAAATTCTTCAACACATTCAAGGGCCTTGTAGTCAACCTTATCTTCAGCTTCAGGGAGCGTGACGAGAGCCTAGAGATCTTTGAGAATTTAAATGATCCAGAGGAAGCTTTGAGGATCATTGAGATTGAGCTCGGCTTTCTCTATGATGCTCTATTCACCAAGATCGCGATTCTTCATACCGGTATTGGAACTGTCTCACGGGTCTTTGCTTCTGGTACCCTTGTGGCTGCATTCATCATTTTTCATAAGAAACCTAACAAAGGCACAGATTTTCATGGAGCTGATGTCGTAGTTACTTATACGCTGTTTGCAGTTGGCCTAGTTTTGGATTTCATATCCATCCTTTTGTTCCTGTTCTCAGACTGGACATGTGCTGCATACAGTAGTTTGAAGGACGATCCGGATGAGCTTCAATCATGGCAAGAGAGATGTTTTAACTGGTTACTTAAATTCAGAAAGCTGCGTTGGACGCCGCAAGAGTGCCACAAGACTGGTATGCATAAGTGTACCAAAGAAGGTTTGAAACCGTGTATCATGAAAGGTGCTGATAAGAAGGAAGGTGGCAATAAGAATGAAGGTGGCAATAAGAAGGAAGGTGACGATAAGAAGGAAGCTGCTGATAAGTGTTCATTGGTAGATAAGCATGACGTTCTCACAACGCGTTTCTTCCTTCGGAGGTGGAGTGGAAGCATCAACGTTTTTAACTTTATAGCGTACGCCACAAAAGCAGATGTAGAGAGGATCCACGACGCCAGAGGTAGTTTCCGCCGCTACTCATGGAAGATCATAACTTTTCCATTCAAAAAACTCAACTTCGTCATAAAGAAAATTTTTGGATCGATTGTCAAGTTGATCAATGAAGTGCACAGACGGATCAGCCACGAAGTCAATGCGTTATCAAGAAAGCATCTTTGGGCTCGTCGTTTTCTTTACCCTATCTATTTCGAGTTCATATCCCGCATCCCTCACTTCATCAAATCAGTGTGGGATATCCTCAGTGAGTTCTTTGACATCTCGGATACGCTTGATATGGTCCACAAGACACTATTTGTACATGGAGAGCCCATGACGAGAGAATTGTGGAAGTTCATCTTCGAAGAGCTGAAAAACAAATCAAAGTATGGAGACAGTCCTGAAAATGCCAAGAGGATATCTTTGGCTCGGGGAGAATGGACTTTAAGAGAGAACCTGCCGGTGGATGCAGAACGTGAAAAACTGGTACGCTATGTCACAAAGGTCGATTATGATCAGAGTCTTTTGATGTGGCACATCGCAACCGAGCTCTGTTACCAACAACATGAGAAAGAGACTATCCCAGAGGGTTATGATGAACAACGCAAGCATTACAGTAACCGCGAGTTCAGCAAAATCATCTCAGACTACATGATGTATCTCCTGATCTTGCAACCCGGTCTGATGTCCGAGGTTGCTGGAATTGGGAAGATCAGATTCAGAGACACATTGGCTGAAACTCACAAGTTCTTCCAGAGGAGGCATATCGAGAACGATAGAAGCGTGGAGACAGCCACCCTAAATATTCTGGACGTCGAAAGTGAAATTGAACCAATGGGAGTGAAGGGAGATCGAAGCAAGTCTGTATTGTTTGACGCGAGCAGGCTAGCCAAAGACCTTGCAGAGATGGAGAAGACACACAACAAAGATAAATGGGAGATACTGAGTAAAGTGTGGGTGGAACTTCTCTGTTATGCAGCCTGTCATTGCGACTCTACGGCTCACGTGGAGCAACTCAGCAGAGGCGGAGAGCTCATAAACTTCGTGTGGCTTCTAATGGCTCACTTCGGACTAACAGATCAGTTCCAGATCAACAAAGGAGATGCCAGAGCCAAACTCATCATAGGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

866

Amino Acids

100.19

Weight (kDa)

8.89

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 483 1.6e-97 Domain of unknown function (DUF4220)
DUF594 PF04578 794 - 847 2.2e-25 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2283
Acc36I ACCTGC 1 cut(s) 1932
Acc65I GGTACC 1 cut(s) 950
AccB1I GGYRCC 2 cut(s) 277, 950
AccII CGCG 5 cut(s) 329, 902, 1396, 2091, 2345
AccIII TCCGGA 1 cut(s) 1135
AciI CCGC 6 cut(s) 1208, 1501, 1504, 1693, 2089, 2493
AclI AACGTT 1 cut(s) 1431
AclWI GGATC 8 cut(s) 539, 824, 857, 1127, 1471, 1484, 1579, 1616
AcsI RAATTY 4 cut(s) 737, 820, 1184, 1563
AcuI CTGAAG 2 cut(s) 763, 769
AcvI CACGTG 1 cut(s) 2473
AcyI GRCGYC 3 cut(s) 1205, 1485, 2280
AfaI GTAC 7 cut(s) 124, 581, 952, 1243, 1451, 1788, 1956
AfiI CCNNNNNNNGG 6 cut(s) 250, 433, 548, 995, 1060, 1930
AflIII ACRYGT 3 cut(s) 358, 1103, 1394
AgeI ACCGGT 1 cut(s) 914
AjnI CCWGG 1 cut(s) 547
AjuI GAANNNNNNNTTGG 4 cut(s) 1183, 1215, 1630, 1662
Alw21I GWGCWC 4 cut(s) 867, 1602, 2019, 2502
Alw26I GTCTC 6 cut(s) 417, 934, 1854, 2036, 2184, 2249
Alw44I GTGCAC 1 cut(s) 1598
AlwI GGATC 8 cut(s) 539, 824, 857, 1127, 1471, 1484, 1579, 1616
Ama87I CYCGRG 1 cut(s) 1896
Aor13HI TCCGGA 1 cut(s) 1135
AoxI GGCC 4 cut(s) 585, 650, 757, 1051
ApaLI GTGCAC 1 cut(s) 1598
ApeKI GCWGC 5 cut(s) 962, 1109, 1195, 1349, 2444
ApoI RAATTY 4 cut(s) 737, 820, 1184, 1563
AsiGI ACCGGT 1 cut(s) 914
Asp718I GGTACC 1 cut(s) 950
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 5 cut(s) 156, 305, 551, 757, 1768
AsuC2I CCSGG 1 cut(s) 2145
AsuHPI GGTGA 2 cut(s) 883, 1343
AsuII TTCGAA 2 cut(s) 683, 1830
AsuNHI GCTAGC 1 cut(s) 2353
AvaI CYCGRG 1 cut(s) 1896
AvaII GGWCC 4 cut(s) 156, 305, 551, 1768
BaeGI GKGCMC 2 cut(s) 280, 1602
BaeI ACNNNNGTAYC 2 cut(s) 942, 975
BamHI GGATCC 1 cut(s) 1476
BanI GGYRCC 2 cut(s) 277, 950
BanII GRGCYC 5 cut(s) 867, 1654, 1800, 2019, 2502
BbrPI CACGTG 1 cut(s) 2473
BbsI GAAGAC 1 cut(s) 2387
Bbv12I GWGCWC 4 cut(s) 867, 1602, 2019, 2502
BbvI GCAGC 5 cut(s) 949, 1096, 1182, 1336, 2456
BccI CCATC 2 cut(s) 1082, 2368
BceAI ACGGC 2 cut(s) 504, 2481
BcgI CGANNNNNNTGC 4 cut(s) 663, 697, 1607, 1641
BciT130I CCWGG 1 cut(s) 549
BciVI GTATCC 1 cut(s) 1747
BclI TGATCA 2 cut(s) 1587, 1981
BcnI CCSGG 1 cut(s) 2145
BcoDI GTCTC 6 cut(s) 417, 934, 1854, 2036, 2184, 2249
BfaI CTAG 3 cut(s) 806, 1055, 2354
BfuAI ACCTGC 1 cut(s) 1932
BfuI GTATCC 1 cut(s) 1747
BglII AGATCT 2 cut(s) 226, 810
BisI GCNGC 7 cut(s) 963, 1110, 1196, 1208, 1350, 1504, 2445
BlsI GCNGC 7 cut(s) 964, 1111, 1197, 1209, 1351, 1505, 2446
Bme1390I CCNGG 2 cut(s) 549, 2145
Bme18I GGWCC 4 cut(s) 156, 305, 551, 1768
BmeT110I CYCGRG 1 cut(s) 1896
BmgT120I GGNCC 5 cut(s) 156, 305, 551, 757, 1768
BmiI GGNNCC 3 cut(s) 279, 952, 1478
BmrFI CCNGG 2 cut(s) 549, 2145
BmsI GCATC 8 cut(s) 304, 676, 871, 1434, 1651, 1704, 1924, 2560
BmtI GCTAGC 1 cut(s) 2357
BpiI GAAGAC 1 cut(s) 2387
BplI GAGNNNNNCTC 2 cut(s) 2568, 2600
BpmI CTGGAG 1 cut(s) 497
Bpu14I TTCGAA 2 cut(s) 683, 1830
BpuEI CTTGAG 1 cut(s) 734
BpuMI CCSGG 1 cut(s) 2145
Bsa29I ATCGAT 1 cut(s) 1574
BsaAI YACGTR 1 cut(s) 2473
BsaBI GATNNNNATC 1 cut(s) 1980
BsaHI GRCGYC 3 cut(s) 1205, 1485, 2280
BsaJI CCNNGG 1 cut(s) 2157
BsaWI WCCGGW 2 cut(s) 914, 1135
BsaXI ACNNNNNCTCC 4 cut(s) 1005, 1035, 1402, 1432
Bsc4I CCNNNNNNNGG 6 cut(s) 250, 433, 548, 995, 1060, 1930
Bse118I RCCGGY 2 cut(s) 914, 1927
Bse1I ACTGG 4 cut(s) 1103, 1178, 1231, 1956
Bse3DI GCAATG 1 cut(s) 2452
Bse8I GATNNNNATC 1 cut(s) 1980
BseAI TCCGGA 1 cut(s) 1135
BseBI CCWGG 1 cut(s) 549
BseCI ATCGAT 1 cut(s) 1574
BseDI CCNNGG 1 cut(s) 2157
BseGI GGATG 5 cut(s) 667, 1074, 1144, 1695, 1939
BseJI GATNNNNATC 1 cut(s) 1980
BseLI CCNNNNNNNGG 6 cut(s) 250, 433, 548, 995, 1060, 1930
BseMI GCAATG 1 cut(s) 2452
BseMII CTCAG 5 cut(s) 1107, 1744, 2124, 2402, 2497
BseNI ACTGG 4 cut(s) 1103, 1178, 1231, 1956
BseRI GAGGAG 4 cut(s) 72, 99, 590, 2239
BseSI GKGCMC 2 cut(s) 280, 1602
BseXI GCAGC 5 cut(s) 949, 1096, 1182, 1336, 2456
Bsh1236I CGCG 5 cut(s) 329, 902, 1396, 2091, 2345
BshFI GGCC 4 cut(s) 587, 652, 759, 1053
BshNI GGYRCC 2 cut(s) 277, 950
BshTI ACCGGT 1 cut(s) 914
BshVI ATCGAT 1 cut(s) 1574
BsiHKAI GWGCWC 4 cut(s) 867, 1602, 2019, 2502
BsiHKCI CYCGRG 1 cut(s) 1896
BsiSI CCGG 4 cut(s) 915, 1136, 1928, 2145
BsiWI CGTACG 1 cut(s) 1449
BslFI GGGAC 2 cut(s) 475, 536
BslI CCNNNNNNNGG 6 cut(s) 250, 433, 548, 995, 1060, 1930
BsmAI GTCTC 6 cut(s) 417, 934, 1854, 2036, 2184, 2249
BsmFI GGGAC 2 cut(s) 475, 536
BsmI GAATGC 1 cut(s) 965
BsnI GGCC 4 cut(s) 587, 652, 759, 1053
BsoBI CYCGRG 1 cut(s) 1896
Bsp119I TTCGAA 2 cut(s) 683, 1830
Bsp1286I GDGCHC 7 cut(s) 280, 867, 1602, 1654, 1800, 2019, 2502
Bsp13I TCCGGA 1 cut(s) 1135
Bsp1407I TGTACA 1 cut(s) 1786
Bsp68I TCGCGA 1 cut(s) 902
BspACI CCGC 6 cut(s) 1208, 1501, 1504, 1693, 2089, 2493
BspANI GGCC 4 cut(s) 587, 652, 759, 1053
BspCNI CTCAG 5 cut(s) 1106, 1743, 2123, 2403, 2496
BspDI ATCGAT 1 cut(s) 1574
BspEI TCCGGA 1 cut(s) 1135
BspFNI CGCG 5 cut(s) 329, 902, 1396, 2091, 2345
BspHI TCATGA 1 cut(s) 1269
BspLI GGNNCC 3 cut(s) 279, 952, 1478
BspMI ACCTGC 1 cut(s) 1932
BspOI GCTAGC 1 cut(s) 2357
BspPI GGATC 8 cut(s) 539, 824, 857, 1127, 1471, 1484, 1579, 1616
BspQI GCTCTTC 1 cut(s) 1827
BspT104I TTCGAA 2 cut(s) 683, 1830
BspT107I GGYRCC 2 cut(s) 277, 950
BsrDI GCAATG 1 cut(s) 2452
BsrFI RCCGGY 2 cut(s) 914, 1927
BsrGI TGTACA 1 cut(s) 1786
BsrI ACTGG 4 cut(s) 1103, 1178, 1231, 1956
BssAI RCCGGY 2 cut(s) 914, 1927
BssECI CCNNGG 1 cut(s) 2157
BssNI GRCGYC 3 cut(s) 1205, 1485, 2280
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 5 cut(s) 928, 1118, 1263, 1865, 2084
Bst6I CTCTTC 1 cut(s) 1827
BstACI GRCGYC 3 cut(s) 1205, 1485, 2280
BstAUI TGTACA 1 cut(s) 1786
BstBAI YACGTR 1 cut(s) 2473
BstBI TTCGAA 2 cut(s) 683, 1830
BstC8I GCNNGC 4 cut(s) 2075, 2347, 2351, 2355
BstENI CCTNNNNNAGG 1 cut(s) 993
BstF5I GGATG 5 cut(s) 667, 1074, 1144, 1695, 1939
BstFNI CGCG 5 cut(s) 329, 902, 1396, 2091, 2345
BstHHI GCGC 1 cut(s) 331
BstMAI GTCTC 6 cut(s) 417, 934, 1854, 2036, 2184, 2249
BstMWI GCNNNNNNNGC 5 cut(s) 191, 275, 1204, 1460, 2097
BstNI CCWGG 1 cut(s) 549
BstNSI RCATGY 2 cut(s) 362, 1107
BstSCI CCNGG 2 cut(s) 547, 2143
BstSLI GKGCMC 2 cut(s) 280, 1602
BstUI CGCG 5 cut(s) 329, 902, 1396, 2091, 2345
BstV1I GCAGC 5 cut(s) 949, 1096, 1182, 1336, 2456
BstV2I GAAGAC 1 cut(s) 2387
BstX2I RGATCY 4 cut(s) 226, 544, 810, 1476
BstXI CCANNNNNNTGG 2 cut(s) 571, 1226
BstYI RGATCY 4 cut(s) 226, 544, 810, 1476
Bsu15I ATCGAT 1 cut(s) 1574
BsuI GTATCC 1 cut(s) 1747
BsuRI GGCC 4 cut(s) 587, 652, 759, 1053
BsuTUI ATCGAT 1 cut(s) 1574
BtgZI GCGATG 1 cut(s) 1990
BtsCI GGATG 5 cut(s) 667, 1074, 1144, 1695, 1939
BtsIMutI CAGTG 2 cut(s) 1722, 1738
BtuMI TCGCGA 1 cut(s) 902
BveI ACCTGC 1 cut(s) 1932
Cac8I GCNNGC 4 cut(s) 2075, 2347, 2351, 2355
CciI TCATGA 1 cut(s) 1269
CfoI GCGC 1 cut(s) 331
Cfr10I RCCGGY 2 cut(s) 914, 1927
Cfr13I GGNCC 5 cut(s) 156, 305, 551, 757, 1768
ClaI ATCGAT 1 cut(s) 1574
CseI GACGC 3 cut(s) 1213, 1493, 2351
Csp6I GTAC 7 cut(s) 123, 580, 951, 1242, 1450, 1787, 1955
CspAI ACCGGT 1 cut(s) 914
CviQI GTAC 7 cut(s) 123, 580, 951, 1242, 1450, 1787, 1955
DraI TTTAAA 1 cut(s) 825
Eam1104I CTCTTC 1 cut(s) 1827
EarI CTCTTC 1 cut(s) 1827
EciI GGCGGA 2 cut(s) 1490, 2508
Ecl136II GAGCTC 3 cut(s) 865, 2017, 2500
Eco147I AGGCCT 1 cut(s) 652
Eco24I GRGCYC 5 cut(s) 867, 1654, 1800, 2019, 2502
Eco32I GATATC 2 cut(s) 1726, 1887
Eco47I GGWCC 4 cut(s) 156, 305, 551, 1768
Eco53kI GAGCTC 3 cut(s) 865, 2017, 2500
Eco57I CTGAAG 2 cut(s) 763, 769
Eco72I CACGTG 1 cut(s) 2473
Eco88I CYCGRG 1 cut(s) 1896
EcoICRI GAGCTC 3 cut(s) 865, 2017, 2500
EcoNI CCTNNNNNAGG 1 cut(s) 993
EcoO109I RGGNCCY 1 cut(s) 757
EcoRII CCWGG 1 cut(s) 547
EcoRV GATATC 2 cut(s) 1726, 1887
EcoT22I ATGCAT 1 cut(s) 1236
EcoT38I GRGCYC 5 cut(s) 867, 1654, 1800, 2019, 2502
FalI AAGNNNNNCTT 2 cut(s) 1811, 1843
FaqI GGGAC 2 cut(s) 475, 536
FauI CCCGC 1 cut(s) 1700
FbaI TGATCA 2 cut(s) 1587, 1981
Fnu4HI GCNGC 7 cut(s) 963, 1110, 1196, 1208, 1350, 1504, 2445
FokI GGATG 5 cut(s) 654, 1061, 1151, 1682, 1946
FriOI GRGCYC 5 cut(s) 867, 1654, 1800, 2019, 2502
Fsp4HI GCNGC 7 cut(s) 963, 1110, 1196, 1208, 1350, 1504, 2445
FspBI CTAG 3 cut(s) 806, 1055, 2354
GlaI GCGC 1 cut(s) 330
GluI GCNGC 7 cut(s) 963, 1110, 1196, 1208, 1350, 1504, 2445
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 4 cut(s) 587, 652, 759, 1053
HapII CCGG 4 cut(s) 915, 1136, 1928, 2145
HgaI GACGC 3 cut(s) 1213, 1493, 2351
HhaI GCGC 1 cut(s) 331
Hin1I GRCGYC 3 cut(s) 1205, 1485, 2280
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HindIII AAGCTT 1 cut(s) 840
HinfI GANTC 7 cut(s) 214, 524, 532, 904, 1987, 2184, 2459
HpaII CCGG 4 cut(s) 915, 1136, 1928, 2145
HphI GGTGA 2 cut(s) 883, 1343
Hpy166II GTNNAC 6 cut(s) 125, 305, 769, 1242, 1600, 1771
Hpy8I GTNNAC 6 cut(s) 125, 305, 769, 1242, 1600, 1771
Hpy99I CGWCG 3 cut(s) 1487, 1659, 2285
HpyCH4III ACNGT 5 cut(s) 928, 1118, 1263, 1865, 2084
HpyCH4IV ACGT 6 cut(s) 121, 1383, 1431, 1942, 2280, 2472
HpyF10VI GCNNNNNNNGC 5 cut(s) 191, 275, 1204, 1460, 2097
HpySE526I ACGT 6 cut(s) 121, 1383, 1431, 1942, 2280, 2472
Hsp92I GRCGYC 3 cut(s) 1205, 1485, 2280
HspAI GCGC 1 cut(s) 329
Kpn2I TCCGGA 1 cut(s) 1135
KpnI GGTACC 1 cut(s) 954
Ksp22I TGATCA 2 cut(s) 1587, 1981
LguI GCTCTTC 1 cut(s) 1827
LmnI GCTCC 4 cut(s) 59, 790, 1013, 2476
Lsp1109I GCAGC 5 cut(s) 949, 1096, 1182, 1336, 2456
LweI GCATC 8 cut(s) 304, 676, 871, 1434, 1651, 1704, 1924, 2560
MaeI CTAG 3 cut(s) 806, 1055, 2354
MaeII ACGT 6 cut(s) 121, 1383, 1431, 1942, 2280, 2472
MaeIII GTNAC 7 cut(s) 794, 1027, 1176, 1331, 1963, 2021, 2084
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 4 cut(s) 226, 544, 810, 1476
MhlI GDGCHC 7 cut(s) 280, 867, 1602, 1654, 1800, 2019, 2502
MluCI AATT 9 cut(s) 187, 384, 737, 820, 1184, 1563, 1811, 2169, 2292
MluI ACGCGT 1 cut(s) 1394
MlyI GAGTC 3 cut(s) 518, 1996, 2453
MmeI TCCRAC 1 cut(s) 1181
Mph1103I ATGCAT 1 cut(s) 1236
MroI TCCGGA 1 cut(s) 1135
MseI TTAA 5 cut(s) 824, 1170, 1182, 1437, 1913
MslI CAYNNNNRTG 1 cut(s) 300
MspI CCGG 4 cut(s) 915, 1136, 1928, 2145
MspR9I CCNGG 2 cut(s) 549, 2145
MunI CAATTG 1 cut(s) 384
Mva1269I GAATGC 1 cut(s) 965
MvaI CCWGG 1 cut(s) 549
MvnI CGCG 5 cut(s) 329, 902, 1396, 2091, 2345
MwoI GCNNNNNNNGC 5 cut(s) 191, 275, 1204, 1460, 2097
NciI CCSGG 1 cut(s) 2145
NheI GCTAGC 1 cut(s) 2353
NlaIV GGNNCC 3 cut(s) 279, 952, 1478
NmeAIII GCCGAG 1 cut(s) 846
NmuCI GTSAC 3 cut(s) 794, 1331, 1963
NruI TCGCGA 1 cut(s) 902
NsiI ATGCAT 1 cut(s) 1236
NspI RCATGY 2 cut(s) 362, 1107
NspV TTCGAA 2 cut(s) 683, 1830
PagI TCATGA 1 cut(s) 1269
PceI AGGCCT 1 cut(s) 652
PciI ACATGT 2 cut(s) 358, 1103
PciSI GCTCTTC 1 cut(s) 1827
PcsI WCGNNNNNNNCGW 1 cut(s) 2247
PctI GAATGC 1 cut(s) 965
PfeI GAWTC 4 cut(s) 214, 532, 904, 2184
Pfl23II CGTACG 1 cut(s) 1449
PfoI TCCNGGA 1 cut(s) 547
PinAI ACCGGT 1 cut(s) 914
PkrI GCNGC 7 cut(s) 964, 1111, 1197, 1209, 1351, 1505, 2446
PleI GAGTC 3 cut(s) 518, 1995, 2453
PmaCI CACGTG 1 cut(s) 2473
PmlI CACGTG 1 cut(s) 2473
PpsI GAGTC 3 cut(s) 518, 1995, 2453
Ppu21I YACGTR 1 cut(s) 2473
PscI ACATGT 2 cut(s) 358, 1103
Psp124BI GAGCTC 3 cut(s) 867, 2019, 2502
Psp1406I AACGTT 1 cut(s) 1431
Psp6I CCWGG 1 cut(s) 547
PspCI CACGTG 1 cut(s) 2473
PspGI CCWGG 1 cut(s) 547
PspLI CGTACG 1 cut(s) 1449
PspN4I GGNNCC 3 cut(s) 279, 952, 1478
PspPI GGNCC 5 cut(s) 156, 305, 551, 757, 1768
PsuI RGATCY 4 cut(s) 226, 544, 810, 1476
RruI TCGCGA 1 cut(s) 902
RsaI GTAC 7 cut(s) 124, 581, 952, 1243, 1451, 1788, 1956
RsaNI GTAC 7 cut(s) 123, 580, 951, 1242, 1450, 1787, 1955
RseI CAYNNNNRTG 1 cut(s) 300
SacI GAGCTC 3 cut(s) 867, 2019, 2502
SapI GCTCTTC 1 cut(s) 1827
SaqAI TTAA 5 cut(s) 824, 1170, 1182, 1437, 1913
SatI GCNGC 7 cut(s) 963, 1110, 1196, 1208, 1350, 1504, 2445
Sau96I GGNCC 5 cut(s) 156, 305, 551, 757, 1768
SchI GAGTC 3 cut(s) 518, 1996, 2453
ScrFI CCNGG 2 cut(s) 549, 2145
SduI GDGCHC 7 cut(s) 280, 867, 1602, 1654, 1800, 2019, 2502
SfaNI GCATC 8 cut(s) 304, 676, 871, 1434, 1651, 1704, 1924, 2560
SfuI TTCGAA 2 cut(s) 683, 1830
SinI GGWCC 4 cut(s) 156, 305, 551, 1768
SmiI ATTTAAAT 1 cut(s) 825
SmiMI CAYNNNNRTG 1 cut(s) 300
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
Sse9I AATT 9 cut(s) 187, 384, 737, 820, 1184, 1563, 1811, 2169, 2292
SseBI AGGCCT 1 cut(s) 652
SsiI CCGC 6 cut(s) 1208, 1501, 1504, 1693, 2089, 2493
SspI AATATT 1 cut(s) 2272
SspMI CTAG 3 cut(s) 806, 1055, 2354
SstI GAGCTC 3 cut(s) 867, 2019, 2502
StuI AGGCCT 1 cut(s) 652
StyD4I CCNGG 2 cut(s) 547, 2143
SwaI ATTTAAAT 1 cut(s) 825
TaaI ACNGT 5 cut(s) 928, 1118, 1263, 1865, 2084
TaiI ACGT 6 cut(s) 124, 1386, 1434, 1945, 2283, 2475
TaqI TCGA 8 cut(s) 683, 1574, 1680, 1830, 1974, 2235, 2283, 2320
TaqII GACCGA 1 cut(s) 722
TasI AATT 9 cut(s) 187, 384, 737, 820, 1184, 1563, 1811, 2169, 2292
TatI WGTACW 2 cut(s) 579, 1786
TauI GCSGC 2 cut(s) 1210, 1506
TfiI GAWTC 4 cut(s) 214, 532, 904, 2184
Tru1I TTAA 5 cut(s) 824, 1170, 1182, 1437, 1913
Tru9I TTAA 5 cut(s) 824, 1170, 1182, 1437, 1913
TscAI CASTG 2 cut(s) 1722, 1738
TseFI GTSAC 3 cut(s) 794, 1331, 1963
TseI GCWGC 5 cut(s) 962, 1109, 1195, 1349, 2444
Tsp45I GTSAC 3 cut(s) 794, 1331, 1963
TspGWI ACGGA 1 cut(s) 1621
TspRI CASTG 2 cut(s) 1722, 1738
VneI GTGCAC 1 cut(s) 1598
VpaK11BI GGWCC 4 cut(s) 156, 305, 551, 1768
XagI CCTNNNNNAGG 1 cut(s) 993
XapI RAATTY 4 cut(s) 737, 820, 1184, 1563
XceI RCATGY 2 cut(s) 362, 1107
XspI CTAG 3 cut(s) 806, 1055, 2354
ZraI GACGTC 1 cut(s) 2281
Zsp2I ATGCAT 1 cut(s) 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.