Rorug02G0182900

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000662
Physical Location & Seq
Reverse (-)
17048385 .. 17057023
8639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0182900.1

Sequence Viewer

Length: 2535 bp
ATGGAAACCCTAAAGCTTCCAATTTCAATCAGACTCCCCAATCACCACTCTCCTCTTCTCAACCACCACCACCACCCAACCACCGCCGCCACTCGTCTCATCTCCCCCAAACCCATTCTCCGCCGCCTCCGCTCTCCTCTCTTTCGGTTCAAACCCTCTCTCCTTTCTCCCCCGCGAAACTTCCCTACTCTTCTTCGTTGCTCTGCCGCCGAGGTCCACTCCGATCACGATCACCACCACCACCACGACGAGCACCGCCACGAGCACGAGCACGACCACGACCACGATCACGGTCACGACCACCACCACCATCATCACCACCACGGCGGAGATTGTGCGGAGCTAACCGGGACTCAGAAAGCGTTCATCGGATTTGCAAAGGCTGTGCGATGGACCGACCTCGCCGATTTCCTCAGGGAGCACTTGCATTTGTGTTTCTGCTCCGCCGCGCTGTTCCTCGCCGCCGCCGCTTGCCCTTACGTGGTTCCCAAGCTCGCCGTGAAGCCTCTGCAGAATGCTTTCCTGATCGTCGCTTTTCCTCTAGTCGGAGTCTCTGCGGCACTTGATGCAATGACTGACATTAGTGGCGGGAAGGTGAACATTCATGTATTAATGGCTTTGGCAGCTTTTGCCTCGGTGTTTATGGGGAATGCTCTTGAAGGAGGCTTGCTTTTGGCTATGTTCAATCTTGCTCATATCGCTGAGGAGTATTTTACCAGTCGCTCCATGATCGATGTGAAAGAGTTGAAAGAAAATTATCCGGATTCTGCCCTTGTTCTGGATATGGATGATGAACAGGTTCCGGATACATCTAATTTGAAGTACAAGCAGGTTCCTGTGCACGACCTACAAGTCGGGTCATATATCTTGGTTGGAGCTGGAGAGTCTGTGCCTGTAGATTGTGAAGTGTTCCAAGGTAATGCTACCATTACTATCGAGCACTTAACGGGAGAAGTTACGCCATTGGAGACAAAAGCTGGAGACATGATACCTGGTGGTGCTAGGAACTTGGATGGCAGGATAATAGTGAAGGCAAGAAAAAATTGGAAAGAGTCAACCCTTAGCAGGATTGTGCAATTGACAGAAGAAGCACAATTAAATAAACCAAAGCTTCAGAGGTGGTTAGATCAGTTTGGCGAGCAATACAGCAAGGTGGTTGTAGTTTTGTCAGTCGCTGTTGCTCTACTTGGTCCATTCCTATTCAAGTGGCCGTTCATTGGTACATCAGCTTGCAGAGGATCAGTTTATAGAGCATTGGGCCTCATGGTGGCAGCATCACCATGTGCTTTGGCTGTAGCTCCATTGGCCTATGCTACTGCAGTTAGCTCCTGTGCAAGGAAGGGTATATTGTTGAAAGGCGGTCATGTGCTAGATGCTCTAGCTTCCTGTCACACTATTGCGTTTGATAAAACTGGGACCTTGACAACTGGGGGCCTAGCGTTTAAAGCAATTGAACCAATTTATGGACATCAAGTTAGAAACAATAAATCAGATTTTTCTTCCTGTTGTGTGCCAAGCTGTGAAAAAGAGGCCCTTGCTGTCGCTGCCGCAATGGAGAAGGGTACCACTCACCCTATTGGAAGAGCTGTTGTAGACCATAGTGAAGGGAAAGACCTTCCTTCTGTTTCTGTTGAGAGTTTCGAGTATTTTCCCGGCAGAGGTCTAGTTGCTACTGTCAATGGCATTGAGTTAGGAACCGAAGGGGGTAAACTGTTGAAAGCATCTCTTGGTTCAGTAGATTTCATCACTTCACTGTGTTTCTCGGAAGATGCATCCAAAAAGATCAAGGAAGCTGTGAATGCTTCTTCTTACGGAACTGATTTTGTTCGTGCTGCTCTTTCAGTTGACGAAAAGGTAACACTGATTCACCTCGAGGATAGGCCTCGACCTGGGGTCTTGGATGTCATTGCAGAGTTACAAAATCAAGCAAAGCTCCGTATAATGATGTTAACTGGTGATCATGAGTCAAGTGCTTGGAGAGTTGCAAATGCTGTAGGTATCAATGAAGTTCATTGCAGCCTGAAGCCTGAGGATAAACTCAGTCATGTCAAGGGTGTCTCAAGGGATATGGGGGGAGGTCTGATAATGGTTGGTGAAGGTATTAATGATGCTCCAGCACTTGCAGCTGCAACGGTAGGGATTGTCCTTGCGCAACGTGCTAGTGCAACTGCCACAGCTGTTGCTGATGTGTTGTTGCTGCGTGACAATATATCTGCCGTGCCATTCTGTATTGCCAAGTCTCGCCAAACAACTTCTCTGGTTAAGCAGAATGTAGTCCTTGCTTTAAGCTGTATAGTTTTGGCCTCTCTTCCATCTGTTTTAGGTTTTCTTCCCCTCTGGTTAACGGTTCTATTACATGAAGGCGGTACCCTTCTTGTCTGCCTCAACTCCATCCGTGCTCTAAACCACCCGACATGGAGCTGGAGGCAGGATTTATGGGATTTATTTAACCAACTGAAATCAAGACTCGAATTGTCAAGGAGACTTGCAACGAGCTCAAACCCCACCCAAGCTGCACCTCCGGTATCCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

844

Amino Acids

91.42

Weight (kDa)

6.78

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1-E2_ATPase PF00122 270 - 362 4.5e-23 P-type ATPase actuator domain
Hydrolase PF00702 465 - 710 2.6e-20 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2533
AasI GACNNNNNNGTC 1 cut(s) 851
AbsI CCTCGAGG 1 cut(s) 1871
Acc16I TGCGCA 1 cut(s) 2151
Acc36I ACCTGC 1 cut(s) 820
Acc65I GGTACC 2 cut(s) 1562, 2366
AccB1I GGYRCC 2 cut(s) 1562, 2366
AccB7I CCANNNNNTGG 1 cut(s) 1463
AccBSI CCGCTC 1 cut(s) 132
AccI GTMKAC 1 cut(s) 1593
AccII CGCG 2 cut(s) 175, 449
AccIII TCCGGA 2 cut(s) 760, 802
AclWI GGATC 1 cut(s) 1246
AcoI YGGCCR 1 cut(s) 1208
AcuI CTGAAG 2 cut(s) 1097, 2042
AdeI CACNNNGTG 1 cut(s) 1283
AfaI GTAC 4 cut(s) 824, 1222, 1564, 2368
AhdI GACNNNNNGTC 1 cut(s) 1892
AjnI CCWGG 2 cut(s) 991, 1888
Alw21I GWGCWC 8 cut(s) 255, 267, 273, 423, 843, 942, 2401, 2498
Alw26I GTCTC 7 cut(s) 101, 556, 962, 975, 2062, 2244, 2476
Alw44I GTGCAC 1 cut(s) 839
AlwI GGATC 1 cut(s) 1246
AlwNI CAGNNNCTG 1 cut(s) 1175
Ama87I CYCGRG 1 cut(s) 1871
Aor13HI TCCGGA 2 cut(s) 760, 802
AoxI GGCC 7 cut(s) 1208, 1258, 1305, 1432, 1530, 1880, 2301
ApaLI GTGCAC 1 cut(s) 839
ApeKI GCWGC 9 cut(s) 623, 1271, 1544, 1832, 2016, 2123, 2126, 2197, 2513
AseI ATTAAT 2 cut(s) 611, 2103
Asp700I GAANNNNTTC 3 cut(s) 362, 518, 798
Asp718I GGTACC 2 cut(s) 1562, 2366
AspLEI GCGC 2 cut(s) 451, 2152
AspS9I GGNCC 7 cut(s) 214, 393, 1190, 1258, 1416, 1432, 1531
AsuC2I CCSGG 2 cut(s) 349, 1653
AsuHPI GGTGA 9 cut(s) 35, 224, 308, 607, 1269, 1562, 1859, 1967, 2105
AvaI CYCGRG 1 cut(s) 1871
AvaII GGWCC 4 cut(s) 214, 393, 1190, 1416
AxyI CCTNAGG 2 cut(s) 413, 2028
BaeGI GKGCMC 1 cut(s) 843
BanI GGYRCC 2 cut(s) 1562, 2366
BanII GRGCYC 1 cut(s) 2498
BauI CACGAG 2 cut(s) 260, 266
Bbv12I GWGCWC 8 cut(s) 255, 267, 273, 423, 843, 942, 2401, 2498
BbvCI CCTCAGC 1 cut(s) 702
BbvI GCAGC 9 cut(s) 635, 1283, 1531, 1819, 2028, 2113, 2135, 2184, 2500
BccI CCATC 5 cut(s) 318, 384, 1007, 2320, 2399
BceAI ACGGC 4 cut(s) 340, 482, 1195, 2201
BciT130I CCWGG 2 cut(s) 993, 1890
BciVI GTATCC 1 cut(s) 799
BclI TGATCA 1 cut(s) 1957
BcnI CCSGG 2 cut(s) 349, 1653
BcoDI GTCTC 7 cut(s) 101, 556, 962, 975, 2062, 2244, 2476
BfaI CTAG 7 cut(s) 542, 1002, 1370, 1379, 1436, 1664, 2160
BfmI CTRYAG 5 cut(s) 509, 894, 1293, 1317, 1992
BfuAI ACCTGC 1 cut(s) 820
BfuI GTATCC 1 cut(s) 799
Bme1390I CCNGG 4 cut(s) 349, 993, 1653, 1890
Bme18I GGWCC 4 cut(s) 214, 393, 1190, 1416
BmeRI GACNNNNNGTC 1 cut(s) 1892
BmeT110I CYCGRG 1 cut(s) 1871
BmgT120I GGNCC 7 cut(s) 214, 393, 1190, 1258, 1416, 1432, 1531
BmiI GGNNCC 8 cut(s) 486, 801, 834, 1417, 1433, 1564, 1696, 2368
BmrFI CCNGG 4 cut(s) 349, 993, 1653, 1890
BmrI ACTGGG 2 cut(s) 1422, 1437
BmsI GCATC 7 cut(s) 556, 1283, 1363, 1730, 1759, 1781, 2098
BmuI ACTGGG 2 cut(s) 1422, 1437
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpmI CTGGAG 4 cut(s) 900, 999, 2097, 2443
Bpu10I CCTNAGC 2 cut(s) 702, 1061
BpuEI CTTGAG 1 cut(s) 2044
BpuMI CCSGG 2 cut(s) 349, 1653
Bsa29I ATCGAT 1 cut(s) 732
BsaAI YACGTR 1 cut(s) 481
BsaBI GATNNNNATC 1 cut(s) 228
BsaJI CCNNGG 5 cut(s) 210, 322, 633, 913, 1889
BsaWI WCCGGW 3 cut(s) 760, 802, 2521
BsaXI ACNNNNNCTCC 6 cut(s) 102, 132, 144, 174, 873, 903
Bse1I ACTGG 4 cut(s) 717, 1417, 1432, 1957
Bse21I CCTNAGG 2 cut(s) 413, 2028
Bse3DI GCAATG 4 cut(s) 576, 1557, 1905, 2011
Bse8I GATNNNNATC 1 cut(s) 228
BseAI TCCGGA 2 cut(s) 760, 802
BseBI CCWGG 2 cut(s) 993, 1890
BseCI ATCGAT 1 cut(s) 732
BseDI CCNNGG 5 cut(s) 210, 322, 633, 913, 1889
BseGI GGATG 5 cut(s) 793, 1018, 1772, 1906, 2391
BseJI GATNNNNATC 1 cut(s) 228
BseMI GCAATG 4 cut(s) 576, 1557, 1905, 2011
BseMII CTCAG 5 cut(s) 368, 427, 693, 2019, 2053
BseNI ACTGG 4 cut(s) 717, 1417, 1432, 1957
BseRI GAGGAG 3 cut(s) 42, 126, 719
BseSI GKGCMC 1 cut(s) 843
BseXI GCAGC 9 cut(s) 635, 1283, 1531, 1819, 2028, 2113, 2135, 2184, 2500
BsgI GTGCAG 1 cut(s) 2499
Bsh1236I CGCG 2 cut(s) 175, 449
BshFI GGCC 7 cut(s) 1210, 1260, 1307, 1434, 1532, 1882, 2303
BshNI GGYRCC 2 cut(s) 1562, 2366
BshVI ATCGAT 1 cut(s) 732
BsiHKAI GWGCWC 8 cut(s) 255, 267, 273, 423, 843, 942, 2401, 2498
BsiHKCI CYCGRG 1 cut(s) 1871
BsiSI CCGG 5 cut(s) 348, 761, 803, 1653, 2522
BslFI GGGAC 2 cut(s) 364, 1429
BsmAI GTCTC 7 cut(s) 101, 556, 962, 975, 2062, 2244, 2476
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 2 cut(s) 364, 1429
BsmI GAATGC 3 cut(s) 520, 655, 1804
BsnI GGCC 7 cut(s) 1210, 1260, 1307, 1434, 1532, 1882, 2303
BsoBI CYCGRG 1 cut(s) 1871
Bsp1286I GDGCHC 8 cut(s) 255, 267, 273, 423, 843, 942, 2401, 2498
Bsp13I TCCGGA 2 cut(s) 760, 802
Bsp143I GATC 9 cut(s) 223, 229, 286, 525, 729, 1126, 1238, 1782, 1957
BspANI GGCC 7 cut(s) 1210, 1260, 1307, 1434, 1532, 1882, 2303
BspCNI CTCAG 5 cut(s) 367, 426, 694, 2020, 2052
BspDI ATCGAT 1 cut(s) 732
BspEI TCCGGA 2 cut(s) 760, 802
BspFNI CGCG 2 cut(s) 175, 449
BspHI TCATGA 1 cut(s) 1960
BspLI GGNNCC 8 cut(s) 486, 801, 834, 1417, 1433, 1564, 1696, 2368
BspMAI CTGCAG 2 cut(s) 513, 1321
BspMI ACCTGC 1 cut(s) 820
BspPI GGATC 1 cut(s) 1246
BspQI GCTCTTC 1 cut(s) 1576
BspT107I GGYRCC 2 cut(s) 1562, 2366
BsrBI CCGCTC 1 cut(s) 132
BsrDI GCAATG 4 cut(s) 576, 1557, 1905, 2011
BsrI ACTGG 4 cut(s) 717, 1417, 1432, 1957
BssECI CCNNGG 5 cut(s) 210, 322, 633, 913, 1889
BssMI GATC 9 cut(s) 223, 229, 286, 525, 729, 1126, 1238, 1782, 1957
BssSI CACGAG 2 cut(s) 260, 266
BssT1I CCWWGG 1 cut(s) 913
Bst2BI CACGAG 2 cut(s) 260, 266
Bst2UI CCWGG 2 cut(s) 993, 1890
Bst4CI ACNGT 6 cut(s) 293, 1675, 1713, 1755, 2134, 2347
Bst6I CTCTTC 4 cut(s) 60, 195, 1576, 2313
BstAPI GCANNNNNTGC 2 cut(s) 566, 629
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 5 cut(s) 472, 495, 668, 1139, 1231
BstDEI CTNAG 6 cut(s) 354, 413, 702, 1061, 2028, 2039
BstDSI CCRYGG 1 cut(s) 322
BstENI CCTNNNNNAGG 1 cut(s) 1333
BstF5I GGATG 5 cut(s) 793, 1018, 1772, 1906, 2391
BstFNI CGCG 2 cut(s) 175, 449
BstHHI GCGC 2 cut(s) 451, 2152
BstKTI GATC 9 cut(s) 226, 232, 289, 528, 732, 1129, 1241, 1785, 1960
BstMAI GTCTC 7 cut(s) 101, 556, 962, 975, 2062, 2244, 2476
BstMBI GATC 9 cut(s) 223, 229, 286, 525, 729, 1126, 1238, 1782, 1957
BstNI CCWGG 2 cut(s) 993, 1890
BstSCI CCNGG 4 cut(s) 347, 991, 1651, 1888
BstSFI CTRYAG 5 cut(s) 509, 894, 1293, 1317, 1992
BstSLI GKGCMC 1 cut(s) 843
BstUI CGCG 2 cut(s) 175, 449
BstV1I GCAGC 9 cut(s) 635, 1283, 1531, 1819, 2028, 2113, 2135, 2184, 2500
Bsu15I ATCGAT 1 cut(s) 732
Bsu36I CCTNAGG 2 cut(s) 413, 2028
BsuI GTATCC 1 cut(s) 799
BsuRI GGCC 7 cut(s) 1210, 1260, 1307, 1434, 1532, 1882, 2303
BsuTUI ATCGAT 1 cut(s) 732
BtgI CCRYGG 1 cut(s) 322
BtgZI GCGATG 1 cut(s) 403
BtsCI GGATG 5 cut(s) 793, 1018, 1772, 1906, 2391
BtsIMutI CAGTG 2 cut(s) 1751, 1859
BveI ACCTGC 1 cut(s) 820
Cac8I GCNNGC 5 cut(s) 472, 495, 668, 1139, 1231
CaiI CAGNNNCTG 1 cut(s) 1175
CciI TCATGA 1 cut(s) 1960
CfoI GCGC 2 cut(s) 451, 2152
Cfr13I GGNCC 7 cut(s) 214, 393, 1190, 1258, 1416, 1432, 1531
ClaI ATCGAT 1 cut(s) 732
CsiI ACCWGGT 1 cut(s) 991
Csp6I GTAC 4 cut(s) 823, 1221, 1563, 2367
CspCI CAANNNNNGTGG 2 cut(s) 2161, 2196
CviQI GTAC 4 cut(s) 823, 1221, 1563, 2367
DdeI CTNAG 6 cut(s) 354, 413, 702, 1061, 2028, 2039
DpnI GATC 9 cut(s) 225, 231, 288, 527, 731, 1128, 1240, 1784, 1959
DpnII GATC 9 cut(s) 223, 229, 286, 525, 729, 1126, 1238, 1782, 1957
DraI TTTAAA 1 cut(s) 1444
DraIII CACNNNGTG 1 cut(s) 1283
DrdI GACNNNNNNGTC 1 cut(s) 851
DriI GACNNNNNGTC 1 cut(s) 1892
DseDI GACNNNNNNGTC 1 cut(s) 851
EaeI YGGCCR 1 cut(s) 1208
Eam1104I CTCTTC 4 cut(s) 60, 195, 1576, 2313
Eam1105I GACNNNNNGTC 1 cut(s) 1892
EarI CTCTTC 4 cut(s) 60, 195, 1576, 2313
EciI GGCGGA 3 cut(s) 110, 342, 433
Ecl136II GAGCTC 1 cut(s) 2496
Eco130I CCWWGG 1 cut(s) 913
Eco147I AGGCCT 1 cut(s) 1882
Eco24I GRGCYC 1 cut(s) 2498
Eco47I GGWCC 4 cut(s) 214, 393, 1190, 1416
Eco53kI GAGCTC 1 cut(s) 2496
Eco57I CTGAAG 2 cut(s) 1097, 2042
Eco81I CCTNAGG 2 cut(s) 413, 2028
Eco88I CYCGRG 1 cut(s) 1871
EcoICRI GAGCTC 1 cut(s) 2496
EcoNI CCTNNNNNAGG 1 cut(s) 1333
EcoO109I RGGNCCY 3 cut(s) 1416, 1432, 1531
EcoRII CCWGG 2 cut(s) 991, 1888
EcoT14I CCWWGG 1 cut(s) 913
EcoT22I ATGCAT 1 cut(s) 1774
EcoT38I GRGCYC 1 cut(s) 2498
ErhI CCWWGG 1 cut(s) 913
Esp3I CGTCTC 1 cut(s) 101
FalI AAGNNNNNCTT 4 cut(s) 1518, 1550, 1710, 1742
FaqI GGGAC 2 cut(s) 364, 1429
FauI CCCGC 2 cut(s) 180, 581
FbaI TGATCA 1 cut(s) 1957
FblI GTMKAC 1 cut(s) 1593
FokI GGATG 5 cut(s) 800, 1025, 1759, 1913, 2378
FriOI GRGCYC 1 cut(s) 2498
FspBI CTAG 7 cut(s) 542, 1002, 1370, 1379, 1436, 1664, 2160
FspI TGCGCA 1 cut(s) 2151
GlaI GCGC 2 cut(s) 450, 2151
GsuI CTGGAG 4 cut(s) 900, 999, 2097, 2443
HaeIII GGCC 7 cut(s) 1210, 1260, 1307, 1434, 1532, 1882, 2303
HapII CCGG 5 cut(s) 348, 761, 803, 1653, 2522
HhaI GCGC 2 cut(s) 451, 2152
Hin6I GCGC 2 cut(s) 449, 2150
HinP1I GCGC 2 cut(s) 449, 2150
HincII GTYRAC 4 cut(s) 1056, 1846, 1950, 2343
HindII GTYRAC 4 cut(s) 1056, 1846, 1950, 2343
HindIII AAGCTT 2 cut(s) 14, 1109
HinfI GANTC 9 cut(s) 33, 352, 549, 764, 884, 1052, 1864, 1964, 2466
HpaI GTTAAC 2 cut(s) 1950, 2343
HpaII CCGG 5 cut(s) 348, 761, 803, 1653, 2522
HphI GGTGA 9 cut(s) 35, 224, 308, 607, 1269, 1562, 1859, 1967, 2105
Hpy166II GTNNAC 9 cut(s) 217, 598, 841, 1056, 1594, 1709, 1846, 1950, 2343
Hpy188I TCNGA 9 cut(s) 32, 223, 357, 371, 548, 1116, 1492, 1765, 2082
Hpy188III TCNNGA 9 cut(s) 227, 296, 523, 656, 761, 779, 803, 1961, 2463
Hpy8I GTNNAC 9 cut(s) 217, 598, 841, 1056, 1594, 1709, 1846, 1950, 2343
Hpy99I CGWCG 2 cut(s) 251, 533
HpyCH4III ACNGT 6 cut(s) 293, 1675, 1713, 1755, 2134, 2347
HpyCH4IV ACGT 2 cut(s) 480, 2155
HpyF3I CTNAG 6 cut(s) 354, 413, 702, 1061, 2028, 2039
HpySE526I ACGT 2 cut(s) 480, 2155
HspAI GCGC 2 cut(s) 449, 2150
Kpn2I TCCGGA 2 cut(s) 760, 802
KpnI GGTACC 2 cut(s) 1566, 2370
Ksp22I TGATCA 1 cut(s) 1957
KspAI GTTAAC 2 cut(s) 1950, 2343
Kzo9I GATC 9 cut(s) 223, 229, 286, 525, 729, 1126, 1238, 1782, 1957
LguI GCTCTTC 1 cut(s) 1576
Lsp1109I GCAGC 9 cut(s) 635, 1283, 1531, 1819, 2028, 2113, 2135, 2184, 2500
LweI GCATC 7 cut(s) 556, 1283, 1363, 1730, 1759, 1781, 2098
MabI ACCWGGT 1 cut(s) 991
MaeI CTAG 7 cut(s) 542, 1002, 1370, 1379, 1436, 1664, 2160
MaeII ACGT 2 cut(s) 480, 2155
MaeIII GTNAC 6 cut(s) 293, 955, 1388, 1855, 1914, 2201
MalI GATC 9 cut(s) 225, 231, 288, 527, 731, 1128, 1240, 1784, 1959
MbiI CCGCTC 1 cut(s) 132
MboI GATC 9 cut(s) 223, 229, 286, 525, 729, 1126, 1238, 1782, 1957
MfeI CAATTG 2 cut(s) 1076, 1449
MhlI GDGCHC 8 cut(s) 255, 267, 273, 423, 843, 942, 2401, 2498
MluCI AATT 9 cut(s) 21, 754, 814, 1042, 1076, 1094, 1449, 1458, 2471
MlyI GAGTC 7 cut(s) 27, 346, 558, 893, 1061, 1973, 2460
MmeI TCCRAC 2 cut(s) 526, 853
Mph1103I ATGCAT 1 cut(s) 1774
MroI TCCGGA 2 cut(s) 760, 802
MroXI GAANNNNTTC 3 cut(s) 362, 518, 798
MslI CAYNNNNRTG 1 cut(s) 1279
MspA1I CMGCKG 2 cut(s) 2126, 2177
MspI CCGG 5 cut(s) 348, 761, 803, 1653, 2522
MspR9I CCNGG 4 cut(s) 349, 993, 1653, 1890
MunI CAATTG 2 cut(s) 1076, 1449
Mva1269I GAATGC 3 cut(s) 520, 655, 1804
MvaI CCWGG 2 cut(s) 993, 1890
MvnI CGCG 2 cut(s) 175, 449
NciI CCSGG 2 cut(s) 349, 1653
NdeII GATC 9 cut(s) 223, 229, 286, 525, 729, 1126, 1238, 1782, 1957
NlaIV GGNNCC 8 cut(s) 486, 801, 834, 1417, 1433, 1564, 1696, 2368
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 3 cut(s) 293, 1388, 2201
NsbI TGCGCA 1 cut(s) 2151
NsiI ATGCAT 1 cut(s) 1774
PaeR7I CTCGAG 1 cut(s) 1871
PagI TCATGA 1 cut(s) 1960
PceI AGGCCT 1 cut(s) 1882
PciSI GCTCTTC 1 cut(s) 1576
PctI GAATGC 3 cut(s) 520, 655, 1804
PdmI GAANNNNTTC 3 cut(s) 362, 518, 798
PfeI GAWTC 2 cut(s) 764, 1864
PflMI CCANNNNNTGG 1 cut(s) 1463
PleI GAGTC 7 cut(s) 27, 346, 557, 892, 1060, 1972, 2460
PpsI GAGTC 7 cut(s) 27, 346, 557, 892, 1060, 1972, 2460
Ppu21I YACGTR 1 cut(s) 481
PpuMI RGGWCCY 1 cut(s) 1416
PshBI ATTAAT 2 cut(s) 611, 2103
PsiI TTATAA 1 cut(s) 2533
Psp124BI GAGCTC 1 cut(s) 2498
Psp5II RGGWCCY 1 cut(s) 1416
Psp6I CCWGG 2 cut(s) 991, 1888
PspGI CCWGG 2 cut(s) 991, 1888
PspN4I GGNNCC 8 cut(s) 486, 801, 834, 1417, 1433, 1564, 1696, 2368
PspPI GGNCC 7 cut(s) 214, 393, 1190, 1258, 1416, 1432, 1531
PspPPI RGGWCCY 1 cut(s) 1416
PspXI VCTCGAGB 1 cut(s) 1871
PstI CTGCAG 2 cut(s) 513, 1321
PstNI CAGNNNCTG 1 cut(s) 1175
PvuII CAGCTG 2 cut(s) 2126, 2177
RsaI GTAC 4 cut(s) 824, 1222, 1564, 2368
RsaNI GTAC 4 cut(s) 823, 1221, 1563, 2367
RseI CAYNNNNRTG 1 cut(s) 1279
SacI GAGCTC 1 cut(s) 2498
SapI GCTCTTC 1 cut(s) 1576
Sau3AI GATC 9 cut(s) 223, 229, 286, 525, 729, 1126, 1238, 1782, 1957
Sau96I GGNCC 7 cut(s) 214, 393, 1190, 1258, 1416, 1432, 1531
SchI GAGTC 7 cut(s) 27, 346, 558, 893, 1061, 1973, 2460
ScrFI CCNGG 4 cut(s) 349, 993, 1653, 1890
SduI GDGCHC 8 cut(s) 255, 267, 273, 423, 843, 942, 2401, 2498
SexAI ACCWGGT 1 cut(s) 991
SfaNI GCATC 7 cut(s) 556, 1283, 1363, 1730, 1759, 1781, 2098
SfcI CTRYAG 5 cut(s) 509, 894, 1293, 1317, 1992
Sfr274I CTCGAG 1 cut(s) 1871
SinI GGWCC 4 cut(s) 214, 393, 1190, 1416
SlaI CTCGAG 1 cut(s) 1871
SmiMI CAYNNNNRTG 1 cut(s) 1279
SmlI CTYRAG 2 cut(s) 1871, 2059
SmoI CTYRAG 2 cut(s) 1871, 2059
Sse9I AATT 9 cut(s) 21, 754, 814, 1042, 1076, 1094, 1449, 1458, 2471
SseBI AGGCCT 1 cut(s) 1882
SspMI CTAG 7 cut(s) 542, 1002, 1370, 1379, 1436, 1664, 2160
SstI GAGCTC 1 cut(s) 2498
StuI AGGCCT 1 cut(s) 1882
StyD4I CCNGG 4 cut(s) 347, 991, 1651, 1888
StyI CCWWGG 1 cut(s) 913
TaaI ACNGT 6 cut(s) 293, 1675, 1713, 1755, 2134, 2347
TaiI ACGT 2 cut(s) 483, 2158
TaqI TCGA 6 cut(s) 732, 936, 1641, 1872, 1885, 2469
TaqII GACCGA 1 cut(s) 410
TasI AATT 9 cut(s) 21, 754, 814, 1042, 1076, 1094, 1449, 1458, 2471
TatI WGTACW 1 cut(s) 822
TauI GCSGC 9 cut(s) 89, 126, 209, 449, 464, 467, 470, 560, 1550
TfiI GAWTC 2 cut(s) 764, 1864
TscAI CASTG 2 cut(s) 1758, 1866
TseFI GTSAC 3 cut(s) 293, 1388, 2201
TseI GCWGC 9 cut(s) 623, 1271, 1544, 1832, 2016, 2123, 2126, 2197, 2513
Tsp45I GTSAC 3 cut(s) 293, 1388, 2201
TspDTI ATGAA 8 cut(s) 355, 593, 807, 1204, 1732, 2000, 2019, 2373
TspGWI ACGGA 3 cut(s) 1827, 1925, 2384
TspRI CASTG 2 cut(s) 1758, 1866
Van91I CCANNNNNTGG 1 cut(s) 1463
VneI GTGCAC 1 cut(s) 839
VpaK11BI GGWCC 4 cut(s) 214, 393, 1190, 1416
VspI ATTAAT 2 cut(s) 611, 2103
XagI CCTNNNNNAGG 1 cut(s) 1333
XhoI CTCGAG 1 cut(s) 1871
XmiI GTMKAC 1 cut(s) 1593
XmnI GAANNNNTTC 3 cut(s) 362, 518, 798
XspI CTAG 7 cut(s) 542, 1002, 1370, 1379, 1436, 1664, 2160
Zsp2I ATGCAT 1 cut(s) 1774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.