Rh2BG239100

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000662
Physical Location & Seq
Reverse (-)
23819366 .. 23821000
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG239100.1

Sequence Viewer

Length: 1635 bp
ATGGATGAGTACTCCTCAAGAAAAGATGCCAATCTTCCAATTAAGATACACATCGAAAGAGGCCGTGGCCCAGAATCCAAGAGTGAGACTGTGGAAGAAGGTGGCGACAATATTAATAATATTGTTCTGGTGCAGCGTGCTTATCAGTTCTTTAAAATCTCCATGGGTCTTATTATAGATCTTCTTTTTGACTTTCATCAGCGGAACGAGAGCCGTGCCTATTTTTTTCAGAGAACCCAAGAAGAGGCTTATACGTTAATCGCAACTGAGCTCAACTTTATGTATGAAGCTCTTTACACCAAAGCTTATGTGGTGCATTCTGTGCGGGGATACATTTTAAGGGCTATATCATTTGGAACGGTTTGTATAGCCCTTGGTTTCTTCTATAAGTTGGACAAACATGGCTTCCATAGGTTTGATGTTAAAGTTACCTATTCCTTGCTCTTGGGTGCCATAGCCTTGGATTTAATAGCTATTTCGATGCTTATCCTATCTGATTGGACTGCCACAATTGGCCAGCGGTACCAGAAGTCTTGTTTAGCCACTATTCTCATGCACAAGCACCACAAGTGCTTGAAGAGATGTTGGTGGGCTACTACTGAAAAAACAACAAAGTGGAATATTTTATTTGGAAGATGGTCTGAATCTGTCTCTACATTCAGCTTCATAGATTATTCTCTCAAAGAATGCGGAAGAGTGCCACCTACAGTTATGGACTATGTAGGCTTTTTGGGCACTAAAATAATCAAAATCATGGGGCTCAAGGATATTCGAGATGAGTTGAAATATAGATCCAGCAAGAAACTCTCCAAGGAATTATGGAATTTCATTTTTGAAGAGTTGAAAAGTAAATCGGAGGTTGCATATCTTCCTGAAACTGGCAGCGAAATATGTTCAGCAAGAGGAGAGTGGATTCTTAAGAACTATCCGGATGTGCCAGAGTATGACAAATTAAGATCGCGAGTTTATCTTGTCGATGTTACTTATGACCGAAGCATTCTGTTGTGGCACATTGCAACCGAGTTGTACTATTACACTGATGCGAACAATGCTGGTGATGATCATCGTGAGTTTAGTAAGATATTGTCTGATTATATGTTATATCTCTTAGTTATGCAGCCTGCTTTGATGTCTTCTGTAGCGGGAATCGGTCAGAAGAGATTTTCTGATACTTCTGCAGAAGCCAAACAATTCTTTGAAAAAAGGGCACTGGGATCGGCTGCAAAACCAGAAGACGCTTGTGGAAAACTGCTCGATGTAAATACAGACATTAAACCCGTTGATGTCAAAGGGAATAGAAGCAAGTCTGTATTGTTTGATGCATGTATTCTGACAAGGGAGCTGAAGAATTTGAAATGGAAATTGATAAGTAAAGTGTGGGTGGAATTACTATCACATGCTGCAAGTCATTGCAGACCAAGTAGCCATGCCCAATTGCTGAGTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTATTACTGGCTCATTTTGGCATAGCAGAGCAGTTCCAAGTGCTAGAGGTCCAAGCAAGAGTAAAACTGATTGTTGAAAAGCCACCGGGTGAATTCTTTTCTTGGTCTGATGAAAGCCACCAGGATGAAAAGCCACATAGTGAATTCAATGAAGAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

544

Amino Acids

62.66

Weight (kDa)

7.6

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 29 - 225 1.1e-38 Domain of unknown function (DUF4220)
DUF594 PF04578 449 - 499 1.9e-22 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 522
AccB1I GGYRCC 2 cut(s) 449, 522
AccII CGCG 1 cut(s) 961
AccIII TCCGGA 1 cut(s) 928
AciI CCGC 5 cut(s) 202, 325, 520, 690, 1142
AclWI GGATC 2 cut(s) 786, 1222
AcoI YGGCCR 1 cut(s) 514
AcsI RAATTY 4 cut(s) 823, 1348, 1565, 1616
AcuI CTGAAG 1 cut(s) 1364
AdeI CACNNNGTG 2 cut(s) 1562, 1613
AfaI GTAC 3 cut(s) 11, 524, 1028
AfiI CCNNNNNNNGG 2 cut(s) 244, 878
AflII CTTAAG 1 cut(s) 917
AgsI TTSAA 8 cut(s) 577, 784, 836, 844, 1199, 1354, 1550, 1621
AjnI CCWGG 1 cut(s) 1593
AluBI AGCT 7 cut(s) 271, 290, 305, 473, 663, 1342, 1456
AluI AGCT 7 cut(s) 271, 290, 305, 473, 663, 1342, 1456
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 2 cut(s) 80, 655
AlwI GGATC 2 cut(s) 786, 1222
Aor13HI TCCGGA 1 cut(s) 928
AoxI GGCC 3 cut(s) 61, 67, 514
ApeKI GCWGC 5 cut(s) 133, 882, 1117, 1220, 1400
ApoI RAATTY 4 cut(s) 823, 1348, 1565, 1616
AseI ATTAAT 1 cut(s) 114
Asp718I GGTACC 1 cut(s) 522
AspS9I GGNCC 2 cut(s) 68, 1522
AsuC2I CCSGG 1 cut(s) 1560
AsuHPI GGTGA 2 cut(s) 1067, 1574
AvaII GGWCC 1 cut(s) 1522
BaeGI GKGCMC 2 cut(s) 737, 1210
BalI TGGCCA 1 cut(s) 516
BanI GGYRCC 2 cut(s) 449, 522
BanII GRGCYC 2 cut(s) 273, 762
BbsI GAAGAC 2 cut(s) 1125, 1239
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 5 cut(s) 145, 894, 1129, 1207, 1387
BccI CCATC 1 cut(s) 630
BceAI ACGGC 2 cut(s) 48, 198
BcgI CGANNNNNNTGC 4 cut(s) 197, 231, 1197, 1231
BciT130I CCWGG 1 cut(s) 1595
BciVI GTATCC 1 cut(s) 323
BclI TGATCA 1 cut(s) 1060
BcnI CCSGG 1 cut(s) 1560
BcoDI GTCTC 2 cut(s) 80, 655
BfaI CTAG 1 cut(s) 1517
BfmI CTRYAG 3 cut(s) 705, 1137, 1176
BfrI CTTAAG 1 cut(s) 917
BfuI GTATCC 1 cut(s) 323
BglII AGATCT 1 cut(s) 178
BisI GCNGC 5 cut(s) 134, 883, 1118, 1221, 1401
BlsI GCNGC 5 cut(s) 135, 884, 1119, 1222, 1402
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 2 cut(s) 1560, 1595
Bme18I GGWCC 1 cut(s) 1522
BmgT120I GGNCC 2 cut(s) 68, 1522
BmiI GGNNCC 2 cut(s) 451, 524
BmrFI CCNGG 2 cut(s) 1560, 1595
BmrI ACTGGG 1 cut(s) 1220
BmsI GCATC 4 cut(s) 16, 471, 1030, 1309
BmuI ACTGGG 1 cut(s) 1220
BpiI GAAGAC 2 cut(s) 1125, 1239
BplI GAGNNNNNCTC 1 cut(s) 31
BpuEI CTTGAG 1 cut(s) 746
BpuMI CCSGG 1 cut(s) 1560
BsaBI GATNNNNATC 4 cut(s) 30, 50, 485, 1062
BsaJI CCNNGG 5 cut(s) 64, 162, 373, 459, 810
BsaWI WCCGGW 1 cut(s) 928
BsaXI ACNNNNNCTCC 2 cut(s) 1443, 1473
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 878
Bse1I ACTGG 3 cut(s) 883, 1215, 1485
Bse3DI GCAATG 2 cut(s) 1011, 1408
Bse8I GATNNNNATC 4 cut(s) 30, 50, 485, 1062
BseAI TCCGGA 1 cut(s) 928
BseBI CCWGG 1 cut(s) 1595
BseDI CCNNGG 5 cut(s) 64, 162, 373, 459, 810
BseGI GGATG 3 cut(s) 10, 937, 1603
BseJI GATNNNNATC 4 cut(s) 30, 50, 485, 1062
BseLI CCNNNNNNNGG 2 cut(s) 244, 878
BseMI GCAATG 2 cut(s) 1011, 1408
BseMII CTCAG 2 cut(s) 258, 1430
BseNI ACTGG 3 cut(s) 883, 1215, 1485
BseRI GAGGAG 2 cut(s) 4, 918
BseSI GKGCMC 2 cut(s) 737, 1210
BseXI GCAGC 5 cut(s) 145, 894, 1129, 1207, 1387
BsgI GTGCAG 1 cut(s) 152
Bsh1236I CGCG 1 cut(s) 961
BshFI GGCC 3 cut(s) 63, 69, 516
BshNI GGYRCC 2 cut(s) 449, 522
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 2 cut(s) 929, 1559
BslI CCNNNNNNNGG 2 cut(s) 244, 878
BsmAI GTCTC 2 cut(s) 80, 655
BsmI GAATGC 3 cut(s) 316, 692, 996
BsnI GGCC 3 cut(s) 63, 69, 516
Bsp1286I GDGCHC 4 cut(s) 273, 737, 762, 1210
Bsp13I TCCGGA 1 cut(s) 928
Bsp143I GATC 5 cut(s) 178, 791, 956, 1060, 1214
Bsp19I CCATGG 1 cut(s) 162
Bsp68I TCGCGA 1 cut(s) 961
BspACI CCGC 5 cut(s) 202, 325, 520, 690, 1142
BspANI GGCC 3 cut(s) 63, 69, 516
BspCNI CTCAG 2 cut(s) 259, 1431
BspEI TCCGGA 1 cut(s) 928
BspFNI CGCG 1 cut(s) 961
BspLI GGNNCC 2 cut(s) 451, 524
BspMAI CTGCAG 1 cut(s) 1180
BspPI GGATC 2 cut(s) 786, 1222
BspT107I GGYRCC 2 cut(s) 449, 522
BspTI CTTAAG 1 cut(s) 917
BsrDI GCAATG 2 cut(s) 1011, 1408
BsrI ACTGG 3 cut(s) 883, 1215, 1485
BssECI CCNNGG 5 cut(s) 64, 162, 373, 459, 810
BssMI GATC 5 cut(s) 178, 791, 956, 1060, 1214
BssT1I CCWWGG 4 cut(s) 162, 373, 459, 810
Bst2UI CCWGG 1 cut(s) 1595
Bst4CI ACNGT 3 cut(s) 91, 361, 709
Bst6I CTCTTC 6 cut(s) 237, 572, 688, 831, 1151, 1620
BstAFI CTTAAG 1 cut(s) 917
BstAPI GCANNNNNTGC 1 cut(s) 322
BstC8I GCNNGC 3 cut(s) 138, 518, 1122
BstDEI CTNAG 3 cut(s) 267, 1108, 1439
BstDSI CCRYGG 2 cut(s) 64, 162
BstF5I GGATG 3 cut(s) 10, 937, 1603
BstFNI CGCG 1 cut(s) 961
BstKTI GATC 5 cut(s) 181, 794, 959, 1063, 1217
BstMAI GTCTC 2 cut(s) 80, 655
BstMBI GATC 5 cut(s) 178, 791, 956, 1060, 1214
BstMWI GCNNNNNNNGC 3 cut(s) 322, 732, 1049
BstNI CCWGG 1 cut(s) 1595
BstNSI RCATGY 2 cut(s) 1326, 1400
BstSCI CCNGG 2 cut(s) 1558, 1593
BstSFI CTRYAG 3 cut(s) 705, 1137, 1176
BstSLI GKGCMC 2 cut(s) 737, 1210
BstUI CGCG 1 cut(s) 961
BstV1I GCAGC 5 cut(s) 145, 894, 1129, 1207, 1387
BstV2I GAAGAC 2 cut(s) 1125, 1239
BstX2I RGATCY 2 cut(s) 178, 791
BstXI CCANNNNNNTGG 1 cut(s) 460
BstYI RGATCY 2 cut(s) 178, 791
BsuI GTATCC 1 cut(s) 323
BsuRI GGCC 3 cut(s) 63, 69, 516
BtgI CCRYGG 2 cut(s) 64, 162
BtsCI GGATG 3 cut(s) 10, 937, 1603
BtsIMutI CAGTG 2 cut(s) 1035, 1208
BtuMI TCGCGA 1 cut(s) 961
Cac8I GCNNGC 3 cut(s) 138, 518, 1122
Cfr13I GGNCC 2 cut(s) 68, 1522
CseI GACGC 1 cut(s) 1244
Csp6I GTAC 3 cut(s) 10, 523, 1027
CviAII CATG 7 cut(s) 163, 401, 553, 754, 1323, 1397, 1427
CviQI GTAC 3 cut(s) 10, 523, 1027
DdeI CTNAG 3 cut(s) 267, 1108, 1439
DpnI GATC 5 cut(s) 180, 793, 958, 1062, 1216
DpnII GATC 5 cut(s) 178, 791, 956, 1060, 1214
DraI TTTAAA 1 cut(s) 154
DraIII CACNNNGTG 2 cut(s) 1562, 1613
EaeI YGGCCR 1 cut(s) 514
Eam1104I CTCTTC 6 cut(s) 237, 572, 688, 831, 1151, 1620
EarI CTCTTC 6 cut(s) 237, 572, 688, 831, 1151, 1620
Ecl136II GAGCTC 1 cut(s) 271
Eco130I CCWWGG 4 cut(s) 162, 373, 459, 810
Eco24I GRGCYC 2 cut(s) 273, 762
Eco47I GGWCC 1 cut(s) 1522
Eco53kI GAGCTC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 1364
EcoICRI GAGCTC 1 cut(s) 271
EcoRI GAATTC 2 cut(s) 1565, 1616
EcoRII CCWGG 1 cut(s) 1593
EcoT14I CCWWGG 4 cut(s) 162, 373, 459, 810
EcoT22I ATGCAT 1 cut(s) 1324
EcoT38I GRGCYC 2 cut(s) 273, 762
ErhI CCWWGG 4 cut(s) 162, 373, 459, 810
FaeI CATG 7 cut(s) 166, 404, 556, 757, 1326, 1400, 1430
FatI CATG 7 cut(s) 162, 400, 552, 753, 1322, 1396, 1426
FauI CCCGC 2 cut(s) 318, 1135
FbaI TGATCA 1 cut(s) 1060
Fnu4HI GCNGC 5 cut(s) 134, 883, 1118, 1221, 1401
FokI GGATG 3 cut(s) 17, 944, 1610
FriOI GRGCYC 2 cut(s) 273, 762
Fsp4HI GCNGC 5 cut(s) 134, 883, 1118, 1221, 1401
FspBI CTAG 1 cut(s) 1517
GluI GCNGC 5 cut(s) 134, 883, 1118, 1221, 1401
HaeIII GGCC 3 cut(s) 63, 69, 516
HapII CCGG 2 cut(s) 929, 1559
HgaI GACGC 1 cut(s) 1244
Hin1II CATG 7 cut(s) 166, 404, 556, 757, 1326, 1400, 1430
HindIII AAGCTT 1 cut(s) 303
HinfI GANTC 4 cut(s) 74, 644, 913, 1146
HpaII CCGG 2 cut(s) 929, 1559
HphI GGTGA 2 cut(s) 1067, 1574
Hpy188I TCNGA 9 cut(s) 231, 496, 643, 856, 1090, 1155, 1168, 1332, 1582
Hpy188III TCNNGA 6 cut(s) 18, 773, 872, 929, 960, 1067
HpyAV CCTTC 1 cut(s) 92
HpyCH4III ACNGT 3 cut(s) 91, 361, 709
HpyCH4IV ACGT 1 cut(s) 254
HpyF10VI GCNNNNNNNGC 3 cut(s) 322, 732, 1049
HpyF3I CTNAG 3 cut(s) 267, 1108, 1439
HpySE526I ACGT 1 cut(s) 254
Hsp92II CATG 7 cut(s) 166, 404, 556, 757, 1326, 1400, 1430
Kpn2I TCCGGA 1 cut(s) 928
KpnI GGTACC 1 cut(s) 526
Ksp22I TGATCA 1 cut(s) 1060
Kzo9I GATC 5 cut(s) 178, 791, 956, 1060, 1214
LmnI GCTCC 1 cut(s) 1339
Lsp1109I GCAGC 5 cut(s) 145, 894, 1129, 1207, 1387
LweI GCATC 4 cut(s) 16, 471, 1030, 1309
MaeI CTAG 1 cut(s) 1517
MaeII ACGT 1 cut(s) 254
MaeIII GTNAC 3 cut(s) 427, 979, 1459
MalI GATC 5 cut(s) 180, 793, 958, 1062, 1216
MboI GATC 5 cut(s) 178, 791, 956, 1060, 1214
MfeI CAATTG 2 cut(s) 510, 1433
MflI RGATCY 2 cut(s) 178, 791
MhlI GDGCHC 4 cut(s) 273, 737, 762, 1210
MlsI TGGCCA 1 cut(s) 516
MluNI TGGCCA 1 cut(s) 516
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 6 cut(s) 25, 53, 238, 850, 896, 1513
Mox20I TGGCCA 1 cut(s) 516
Mph1103I ATGCAT 1 cut(s) 1324
MroI TCCGGA 1 cut(s) 928
MscI TGGCCA 1 cut(s) 516
MslI CAYNNNNRTG 1 cut(s) 1596
Msp20I TGGCCA 1 cut(s) 516
MspA1I CMGCKG 2 cut(s) 202, 520
MspCI CTTAAG 1 cut(s) 917
MspI CCGG 2 cut(s) 929, 1559
MspR9I CCNGG 2 cut(s) 1560, 1595
MunI CAATTG 2 cut(s) 510, 1433
Mva1269I GAATGC 3 cut(s) 316, 692, 996
MvaI CCWGG 1 cut(s) 1595
MvnI CGCG 1 cut(s) 961
MwoI GCNNNNNNNGC 3 cut(s) 322, 732, 1049
NciI CCSGG 1 cut(s) 1560
NcoI CCATGG 1 cut(s) 162
NdeII GATC 5 cut(s) 178, 791, 956, 1060, 1214
NlaIII CATG 7 cut(s) 166, 404, 556, 757, 1326, 1400, 1430
NlaIV GGNNCC 2 cut(s) 451, 524
NmuCI GTSAC 1 cut(s) 1459
NruI TCGCGA 1 cut(s) 961
NsiI ATGCAT 1 cut(s) 1324
NspI RCATGY 2 cut(s) 1326, 1400
PctI GAATGC 3 cut(s) 316, 692, 996
PfeI GAWTC 4 cut(s) 74, 644, 913, 1146
PkrI GCNGC 5 cut(s) 135, 884, 1119, 1222, 1402
PshBI ATTAAT 1 cut(s) 114
Psp124BI GAGCTC 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 1593
PspGI CCWGG 1 cut(s) 1593
PspN4I GGNNCC 2 cut(s) 451, 524
PspPI GGNCC 2 cut(s) 68, 1522
PstI CTGCAG 1 cut(s) 1180
PsuI RGATCY 2 cut(s) 178, 791
RruI TCGCGA 1 cut(s) 961
RsaI GTAC 3 cut(s) 11, 524, 1028
RsaNI GTAC 3 cut(s) 10, 523, 1027
RseI CAYNNNNRTG 1 cut(s) 1596
SacI GAGCTC 1 cut(s) 273
SatI GCNGC 5 cut(s) 134, 883, 1118, 1221, 1401
Sau3AI GATC 5 cut(s) 178, 791, 956, 1060, 1214
Sau96I GGNCC 2 cut(s) 68, 1522
ScaI AGTACT 1 cut(s) 11
ScrFI CCNGG 2 cut(s) 1560, 1595
SduI GDGCHC 4 cut(s) 273, 737, 762, 1210
SfaNI GCATC 4 cut(s) 16, 471, 1030, 1309
SfcI CTRYAG 3 cut(s) 705, 1137, 1176
SinI GGWCC 1 cut(s) 1522
SmiMI CAYNNNNRTG 1 cut(s) 1596
SmlI CTYRAG 3 cut(s) 16, 761, 917
SmoI CTYRAG 3 cut(s) 16, 761, 917
SsiI CCGC 5 cut(s) 202, 325, 520, 690, 1142
SspI AATATT 3 cut(s) 112, 121, 622
SspMI CTAG 1 cut(s) 1517
SstI GAGCTC 1 cut(s) 273
StyD4I CCNGG 2 cut(s) 1558, 1593
StyI CCWWGG 4 cut(s) 162, 373, 459, 810
TaaI ACNGT 3 cut(s) 91, 361, 709
TaiI ACGT 1 cut(s) 257
TaqI TCGA 5 cut(s) 54, 479, 772, 975, 1254
TaqII GACCGA 2 cut(s) 1005, 1139
TatI WGTACW 2 cut(s) 9, 1026
TfiI GAWTC 4 cut(s) 74, 644, 913, 1146
TscAI CASTG 2 cut(s) 1042, 1215
TseFI GTSAC 1 cut(s) 1459
TseI GCWGC 5 cut(s) 133, 882, 1117, 1220, 1400
Tsp45I GTSAC 1 cut(s) 1459
TspDTI ATGAA 6 cut(s) 185, 300, 655, 817, 1599, 1614
TspRI CASTG 2 cut(s) 1042, 1215
Vha464I CTTAAG 1 cut(s) 917
VpaK11BI GGWCC 1 cut(s) 1522
VspI ATTAAT 1 cut(s) 114
XapI RAATTY 4 cut(s) 823, 1348, 1565, 1616
XceI RCATGY 2 cut(s) 1326, 1400
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XspI CTAG 1 cut(s) 1517
ZrmI AGTACT 1 cut(s) 11
Zsp2I ATGCAT 1 cut(s) 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.