Rh2BG250700

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000662
Physical Location & Seq
Reverse (-)
25691610 .. 25692740
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG250700.1

Sequence Viewer

Length: 1131 bp
ATGCTGATATTATCTGATTGGACGATCGCTGTTATATACAAGTACTGTGAAAAGTGTTGTGTAGTCATTGATCTCATGATGAAGTACCTGGAGTTTAAGAGATCATGGCGACCAACTACGCCACCAAAGTATCTGGAGCGGACCAGAAAGATTTTATTTCGAAGATGGTGTGAATTTGTCTCCGGTTTCAGCTTCATAGATTATTCTCTCCGAGAATGTGGAAGATCAGTAGTCCGACCTGCAATTTTGGACGGCATTTACCGAAGGATCATCAGATTTTTGGGCTACCTTTACCATAGAATCATCATATTCCTAGGCCACCTTTACCTCAGAATCATTAAATTCTTTGGCCTTAAGTATCTTCGAGATGAAATGAAGTACAGATCCAGCAAGAGACTGGAAGAGAATTTGTGGAAATTCATTTTTGATGAGTTGAAAAGTAAATCCTCAGAAGTTGATGATCCCAAAATTGGTATAGAAATATGTTCGTCCCAAGGGGATTGGGTTCTCCTAAAAAAGTATGAGAAGCATCCGGAATACAAGAGCTTACTATCCTATGAGGAATTACAATCGTGCTTTGTTGATGTTACTGATGACCGACGCGTTCTGCTGTGGCATATTGCTACTGAGATGTGCTTTTACACAGATGATAATATGAGCAATGTGCATCGTGACTTTAGTAAGACGTTGTCTGATTATATGCTATATCTCTTAATAATACAGCCTGCTTTGATGTCCTCTGTAGCGGGAATCGGACAAAAGAGATTTAGAGATACGGTTGCAGAAGCCAATAAATTCTTTAAAAAAAGGGGATTGGGTTCCGATGTGAAACTAGGCGAGAAAACACTACGCGAGGCTTGTTGCAAACTCCTTGATGCAGATACAGGAGTTAAACCAGTTGATGTCAAAGGGAATAGAAGTAAATCTGTTTTGTTTGAAGCATGTATTTTGGCCAGGCAGCTGATGAAATTCAAAGAAAAGAAATGGGAATTAATAAGCTCGGTGTGGGTGGAATTGCTATCATACGCTGCAAGTCATTGCAGAGCACATAGCCACGCGCAATTGCTGACTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTACGTACTGATGGCTCGCTTTGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

376

Amino Acids

44.33

Weight (kDa)

9.22

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF594 PF04578 325 - 368 7.1e-19 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 247
AccBSI CCGCTC 1 cut(s) 139
AccII CGCG 3 cut(s) 603, 852, 1058
AccIII TCCGGA 1 cut(s) 532
AciI CCGC 2 cut(s) 139, 746
AclWI GGATC 3 cut(s) 275, 378, 455
AcoI YGGCCR 1 cut(s) 951
AcsI RAATTY 6 cut(s) 173, 341, 406, 416, 794, 968
AfaI GTAC 4 cut(s) 44, 86, 380, 1108
AfiI CCNNNNNNNGG 1 cut(s) 470
AflII CTTAAG 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 3 cut(s) 436, 938, 973
AjnI CCWGG 2 cut(s) 87, 953
AluBI AGCT 5 cut(s) 192, 546, 961, 999, 1084
AluI AGCT 5 cut(s) 192, 546, 961, 999, 1084
Alw21I GWGCWC 1 cut(s) 1048
Alw26I GTCTC 2 cut(s) 184, 388
AlwI GGATC 3 cut(s) 275, 378, 455
Aor13HI TCCGGA 1 cut(s) 532
AoxI GGCC 3 cut(s) 316, 349, 951
ApeKI GCWGC 2 cut(s) 958, 1028
ApoI RAATTY 6 cut(s) 173, 341, 406, 416, 794, 968
AseI ATTAAT 1 cut(s) 992
AspA2I CCTAGG 1 cut(s) 313
AspLEI GCGC 1 cut(s) 1060
AspS9I GGNCC 1 cut(s) 141
AsuII TTCGAA 1 cut(s) 160
AvaII GGWCC 1 cut(s) 141
AvrII CCTAGG 1 cut(s) 313
BaeI ACNNNNGTAYC 4 cut(s) 113, 146, 873, 906
BalI TGGCCA 1 cut(s) 953
Bbv12I GWGCWC 1 cut(s) 1048
BbvI GCAGC 2 cut(s) 970, 1015
BccI CCATC 2 cut(s) 159, 1106
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 2 cut(s) 89, 955
BcoDI GTCTC 2 cut(s) 184, 388
BfaI CTAG 2 cut(s) 314, 833
BfmI CTRYAG 1 cut(s) 741
BfrI CTTAAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 247
BisI GCNGC 2 cut(s) 959, 1029
BlnI CCTAGG 1 cut(s) 313
BlsI GCNGC 2 cut(s) 960, 1030
BmcAI AGTACT 1 cut(s) 44
Bme1390I CCNGG 2 cut(s) 89, 955
Bme18I GGWCC 1 cut(s) 141
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 1 cut(s) 820
BmrFI CCNGG 2 cut(s) 89, 955
BmsI GCATC 3 cut(s) 538, 676, 865
BpmI CTGGAG 2 cut(s) 110, 155
Bpu14I TTCGAA 1 cut(s) 160
BsaAI YACGTR 1 cut(s) 1106
BsaJI CCNNGG 2 cut(s) 313, 493
BsaWI WCCGGW 2 cut(s) 182, 532
BsaXI ACNNNNNCTCC 2 cut(s) 1071, 1101
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 402, 896
Bse3DI GCAATG 2 cut(s) 667, 1036
BseAI TCCGGA 1 cut(s) 532
BseBI CCWGG 2 cut(s) 89, 955
BseDI CCNNGG 2 cut(s) 313, 493
BseGI GGATG 1 cut(s) 529
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMI GCAATG 2 cut(s) 667, 1036
BseMII CTCAG 3 cut(s) 343, 462, 618
BseNI ACTGG 2 cut(s) 402, 896
BseXI GCAGC 2 cut(s) 970, 1015
Bsh1236I CGCG 3 cut(s) 603, 852, 1058
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 3 cut(s) 318, 351, 953
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 1048
BsiSI CCGG 2 cut(s) 183, 533
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 2 cut(s) 184, 388
BsmFI GGGAC 1 cut(s) 475
BsnI GGCC 3 cut(s) 318, 351, 953
Bsp119I TTCGAA 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 1048
Bsp13I TCCGGA 1 cut(s) 532
Bsp143I GATC 7 cut(s) 24, 70, 101, 224, 267, 383, 460
BspACI CCGC 2 cut(s) 139, 746
BspANI GGCC 3 cut(s) 318, 351, 953
BspCNI CTCAG 3 cut(s) 342, 461, 619
BspEI TCCGGA 1 cut(s) 532
BspFNI CGCG 3 cut(s) 603, 852, 1058
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 820
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 3 cut(s) 275, 378, 455
BspT104I TTCGAA 1 cut(s) 160
BspTI CTTAAG 1 cut(s) 353
BsrBI CCGCTC 1 cut(s) 139
BsrDI GCAATG 2 cut(s) 667, 1036
BsrI ACTGG 2 cut(s) 402, 896
BssECI CCNNGG 2 cut(s) 313, 493
BssMI GATC 7 cut(s) 24, 70, 101, 224, 267, 383, 460
BssT1I CCWWGG 2 cut(s) 313, 493
Bst2UI CCWGG 2 cut(s) 89, 955
Bst4CI ACNGT 2 cut(s) 47, 778
Bst6I CTCTTC 1 cut(s) 396
BstAFI CTTAAG 1 cut(s) 353
BstBAI YACGTR 1 cut(s) 1106
BstBI TTCGAA 1 cut(s) 160
BstC8I GCNNGC 2 cut(s) 726, 1118
BstDEI CTNAG 3 cut(s) 329, 448, 627
BstF5I GGATG 1 cut(s) 529
BstFNI CGCG 3 cut(s) 603, 852, 1058
BstHHI GCGC 1 cut(s) 1060
BstKTI GATC 7 cut(s) 27, 73, 104, 227, 270, 386, 463
BstMAI GTCTC 2 cut(s) 184, 388
BstMBI GATC 7 cut(s) 24, 70, 101, 224, 267, 383, 460
BstMCI CGRYCG 1 cut(s) 27
BstNI CCWGG 2 cut(s) 89, 955
BstNSI RCATGY 1 cut(s) 945
BstSCI CCNGG 2 cut(s) 87, 953
BstSFI CTRYAG 1 cut(s) 741
BstSNI TACGTA 1 cut(s) 1106
BstUI CGCG 3 cut(s) 603, 852, 1058
BstV1I GCAGC 2 cut(s) 970, 1015
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
BsuRI GGCC 3 cut(s) 318, 351, 953
BtsCI GGATG 1 cut(s) 529
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 2 cut(s) 726, 1118
CciI TCATGA 1 cut(s) 75
CfoI GCGC 1 cut(s) 1060
Cfr13I GGNCC 1 cut(s) 141
CseI GACGC 1 cut(s) 609
Csp6I GTAC 4 cut(s) 43, 85, 379, 1107
CviAII CATG 3 cut(s) 76, 105, 942
CviQI GTAC 4 cut(s) 43, 85, 379, 1107
DdeI CTNAG 3 cut(s) 329, 448, 627
DpnI GATC 7 cut(s) 26, 72, 103, 226, 269, 385, 462
DpnII GATC 7 cut(s) 24, 70, 101, 224, 267, 383, 460
DraI TTTAAA 1 cut(s) 802
EaeI YGGCCR 1 cut(s) 951
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco105I TACGTA 1 cut(s) 1106
Eco130I CCWWGG 2 cut(s) 313, 493
Eco47I GGWCC 1 cut(s) 141
EcoRII CCWGG 2 cut(s) 87, 953
EcoT14I CCWWGG 2 cut(s) 313, 493
ErhI CCWWGG 2 cut(s) 313, 493
FaeI CATG 3 cut(s) 79, 108, 945
FaqI GGGAC 1 cut(s) 475
FatI CATG 3 cut(s) 75, 104, 941
FauI CCCGC 1 cut(s) 739
Fnu4HI GCNGC 2 cut(s) 959, 1029
FokI GGATG 1 cut(s) 516
Fsp4HI GCNGC 2 cut(s) 959, 1029
FspBI CTAG 2 cut(s) 314, 833
GlaI GCGC 1 cut(s) 1059
GluI GCNGC 2 cut(s) 959, 1029
GsuI CTGGAG 2 cut(s) 110, 155
HaeIII GGCC 3 cut(s) 318, 351, 953
HapII CCGG 2 cut(s) 183, 533
HgaI GACGC 1 cut(s) 609
HhaI GCGC 1 cut(s) 1060
Hin1II CATG 3 cut(s) 79, 108, 945
Hin6I GCGC 1 cut(s) 1058
HinP1I GCGC 1 cut(s) 1058
HinfI GANTC 3 cut(s) 300, 333, 750
HpaII CCGG 2 cut(s) 183, 533
Hpy188I TCNGA 9 cut(s) 16, 212, 236, 275, 332, 451, 694, 755, 823
Hpy188III TCNNGA 5 cut(s) 76, 134, 365, 533, 671
Hpy99I CGWCG 1 cut(s) 603
HpyAV CCTTC 1 cut(s) 258
HpyCH4III ACNGT 2 cut(s) 47, 778
HpyCH4IV ACGT 2 cut(s) 686, 1105
HpyCH4V TGCA 7 cut(s) 242, 667, 782, 864, 878, 1031, 1041
HpyF3I CTNAG 3 cut(s) 329, 448, 627
HpySE526I ACGT 2 cut(s) 686, 1105
Hsp92II CATG 3 cut(s) 79, 108, 945
HspAI GCGC 1 cut(s) 1058
Kpn2I TCCGGA 1 cut(s) 532
Kzo9I GATC 7 cut(s) 24, 70, 101, 224, 267, 383, 460
LmnI GCTCC 1 cut(s) 136
Lsp1109I GCAGC 2 cut(s) 970, 1015
LweI GCATC 3 cut(s) 538, 676, 865
MaeI CTAG 2 cut(s) 314, 833
MaeII ACGT 2 cut(s) 686, 1105
MaeIII GTNAC 4 cut(s) 586, 671, 1087, 1101
MalI GATC 7 cut(s) 26, 72, 103, 226, 269, 385, 462
MbiI CCGCTC 1 cut(s) 139
MboI GATC 7 cut(s) 24, 70, 101, 224, 267, 383, 460
MboII GAAGA 4 cut(s) 174, 234, 353, 413
MfeI CAATTG 1 cut(s) 1061
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 1048
MlsI TGGCCA 1 cut(s) 953
MluI ACGCGT 1 cut(s) 601
MluNI TGGCCA 1 cut(s) 953
MmeI TCCRAC 1 cut(s) 259
MnlI CCTC 5 cut(s) 338, 457, 553, 748, 847
Mox20I TGGCCA 1 cut(s) 953
MroI TCCGGA 1 cut(s) 532
MscI TGGCCA 1 cut(s) 953
MseI TTAA 7 cut(s) 96, 339, 354, 713, 801, 891, 992
Msp20I TGGCCA 1 cut(s) 953
MspA1I CMGCKG 1 cut(s) 961
MspCI CTTAAG 1 cut(s) 353
MspI CCGG 2 cut(s) 183, 533
MspR9I CCNGG 2 cut(s) 89, 955
MunI CAATTG 1 cut(s) 1061
MvaI CCWGG 2 cut(s) 89, 955
MvnI CGCG 3 cut(s) 603, 852, 1058
NdeII GATC 7 cut(s) 24, 70, 101, 224, 267, 383, 460
NlaIII CATG 3 cut(s) 79, 108, 945
NlaIV GGNNCC 1 cut(s) 820
NmuCI GTSAC 2 cut(s) 671, 1087
NspI RCATGY 1 cut(s) 945
NspV TTCGAA 1 cut(s) 160
PagI TCATGA 1 cut(s) 75
PfeI GAWTC 3 cut(s) 300, 333, 750
PflFI GACNNNGTC 1 cut(s) 688
PkrI GCNGC 2 cut(s) 960, 1030
Ple19I CGATCG 1 cut(s) 27
Ppu21I YACGTR 1 cut(s) 1106
PshBI ATTAAT 1 cut(s) 992
Psp6I CCWGG 2 cut(s) 87, 953
PspGI CCWGG 2 cut(s) 87, 953
PspN4I GGNNCC 1 cut(s) 820
PspPI GGNCC 1 cut(s) 141
PsuI RGATCY 1 cut(s) 383
PsyI GACNNNGTC 1 cut(s) 688
PvuI CGATCG 1 cut(s) 27
PvuII CAGCTG 1 cut(s) 961
RsaI GTAC 4 cut(s) 44, 86, 380, 1108
RsaNI GTAC 4 cut(s) 43, 85, 379, 1107
SaqAI TTAA 7 cut(s) 96, 339, 354, 713, 801, 891, 992
SatI GCNGC 2 cut(s) 959, 1029
Sau3AI GATC 7 cut(s) 24, 70, 101, 224, 267, 383, 460
Sau96I GGNCC 1 cut(s) 141
ScaI AGTACT 1 cut(s) 44
ScrFI CCNGG 2 cut(s) 89, 955
SduI GDGCHC 1 cut(s) 1048
SfaNI GCATC 3 cut(s) 538, 676, 865
SfcI CTRYAG 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 160
SinI GGWCC 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SnaBI TACGTA 1 cut(s) 1106
SsiI CCGC 2 cut(s) 139, 746
SspMI CTAG 2 cut(s) 314, 833
StyD4I CCNGG 2 cut(s) 87, 953
StyI CCWWGG 2 cut(s) 313, 493
TaaI ACNGT 2 cut(s) 47, 778
TaiI ACGT 2 cut(s) 689, 1108
TaqI TCGA 2 cut(s) 160, 364
TaqII GACCGA 1 cut(s) 612
TatI WGTACW 2 cut(s) 42, 378
TfiI GAWTC 3 cut(s) 300, 333, 750
Tru1I TTAA 7 cut(s) 96, 339, 354, 713, 801, 891, 992
Tru9I TTAA 7 cut(s) 96, 339, 354, 713, 801, 891, 992
TseFI GTSAC 2 cut(s) 671, 1087
TseI GCWGC 2 cut(s) 958, 1028
Tsp45I GTSAC 2 cut(s) 671, 1087
TspDTI ATGAA 6 cut(s) 95, 184, 384, 389, 409, 980
Tth111I GACNNNGTC 1 cut(s) 688
Vha464I CTTAAG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 141
VspI ATTAAT 1 cut(s) 992
XapI RAATTY 6 cut(s) 173, 341, 406, 416, 794, 968
XceI RCATGY 1 cut(s) 945
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XmaJI CCTAGG 1 cut(s) 313
XspI CTAG 2 cut(s) 314, 833
ZrmI AGTACT 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.