RLG00000020583

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000662
Physical Location & Seq
Forward (+)
66449324 .. 66454771
5448 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020583

Sequence Viewer

Length: 2631 bp
ATGGCCTCCAAGCCCCCCGACGAGTCTTGCATCTCCAAGATTGAGGCGACTACTGTGACTACTGCGTTCGGATCTGCGACTACTACGTTTGCTTTTGGATCGCAGTGCACAAGACGAGAACGGCGGAGCTCGGATTGTTGGACTGAAACTAGAGGGGAGCCAAAGGAGGATATGGTTACTCTCATTCCCGACAGTGTTAGGAATCTATGGGAAAGGTGGAATCTCCGGAGCTTTGTCCTCTTAAGCCTCTCATTGCAGACCACTTTAATTCTTTTTGCACCATTCAGAAAGAGGACACCCAACACATGGCTAACCTTGGTCATCTGGTCAGCGTACTTACTTGCTGATTGGGCTGCTAACTTTGCAGTTGGATTAATCTCAAACAGCCAAGGTGACAATTCAGTTTCCGATGAGGCCAGTGATCTTTTGGTATTTTGGACTCCCTTTCTTTTGTTACACCTTGGTGGTCCAGACACCATCACTGCTTTTGCTCTGGAAGATAATACCCTGTGGCTAAGGCACTTGCTTGGCCTCGTATTCCAGGTTACAGCTTCTGTTTATCTCTTCCTTCAGTCACTACCGAATAAGCTGTGGATACCAACAGGGCTTCTCTTTGTTGCTGGAATCATTAAATATGCAGAGAGAACACGTGCTTTGTATCTTGCAAGCTTGGATAACTTCAAGGAATCCATGCTTAAGAAGCCTGATGCTGGACCTAATTATGCGAAGCTTATGGAAGAATACTCCTCAAAGCTAGATGCCCAGCTTCCAACTCATATTGTACTCACAGCAGAACGCAGCAAAGAATCCAGGACAATAACTTATGTCATTGAAAAAGGTGATATGGAGAATGACATTGCAGTGGTACGACATGCTCATCACTTCTTCCAAATCTTCAAGGGACTTATTATAGATCTCATTTTTAGCTTTCATGAACGGTATGAGAGCCGTGTAATTTTCCATGAGAGAACCTCAGAAGAAGCTTTCAAATTAATCGCGGTTGAGCTCAACTTTATATATGAAGCTCTCTTCACCAAGGTGGTTGTGGTGCATTCTAAGCTAGGATACTTCTTCCGGGCTATAGCCTTTGGTGCACTTTCTGTAGCTCTTGTTTTCTTCTATAAATTAGAGAAAACGGATTTGCATAGGTATGATGTTAGAATTACCTATGGGTTGCTTTATGGTGCCATAGGCCTGGATTCATTATCTGCTTTCATGGTTCTATTCTCCGATTGGACTGTAACAGCCCTGAACAAGTCTTGGCAAGACTCTTGCATAGCTGCTGCTATTCTAGGGAAGTATCTCCATCTTAAGAGATCGTGGTGGTTTACAAAAACAAAAAATGGCAGTGATCAGACTCCGATTGCAAAATCAACAAACTGTTCGGAGTGGACTAGAGAAGTTCTGTTTCGGAGATGGTATGAATCTGTGTCAGCATTCAACTTGATACATTATTCCCTTAAGGAATGTCCAAAAATTTCCCCTACAATTTTGGACTACTTTGGGATTTTCTACGTTAAAATCATTGACTTATTGGGTGTTAAGGATCTTCGAGATAGGATGATATACAGGTCCAGCAAGCCACTAACTAAAGATTTATGGGATTTCATTTTTAAAGAGGTGAAAAGTAAATCAGTGCTGGCAGATGATCCTGAAACTGCGAAGAAGATATGTTCGGCCAGAGGTGACTGGGTTCTCCAAGATAGTGATTGGACTCTCCGAGATAGCTATTGGGACAGTACTGTGGACAAAAAATTGGTGATCAGTAAGTTATTATCTTATGTTGTTGATGTTGATTATGACCAAAGCATTCTATTATGGCACATTGCTACTGAGTTTTGCTACAACATGGATGATTACAATGATGCTAGTGATGATAATGATAGTGGTATTGCTGGAGAGGCTATTGCTGATAGTGGCAGAATCAACAATTTCTTTTCAAAACTTTCAAGACCATTACATATAGGTCTAGAGTTTATTAAGAGATTAAAAGCAGCGATATGGGGGCTTGGGGAGGCCGTGGCCACTTTTTGCCAAAATCTAGTTTCACGTCAAATTGCTGGTGCACAACATGATGAATGTGCTGGTGCTGCTGATGATGATAGTGATGATAGTGTCCATTATTTTAGTAAGAGAAAAGAATACAGTAAGACACTGTCTGATTACATGTTATATCTCCTAGTTATGCAGCCTACTTTGATGTCTTCTGTACCAGGAATTGGACAAATAAGATTCCAAGATACGTGTGAAGAAGCCAAAAAGTTCTTCAGCAGAAGGGAATTGAGTTCTGGAGTGAAAGAGGCTTGTAAAAGAATCCTTGATGTAAATACTGATGTCAAACCTGTTGATGTCAAAGGGGATAGAAGTAAATCTGTTTTGTTTGATGCATGCATTTTGGCCAAAGAGCTGCAGAGGTTGAAGGAAAATAAATGGGATTTAATAAGTGAAGTGTGGGTGGAATTGTTGTCATTTGCTGCAGGGCATTGCAGAGCAAATGACCATGCTCAATTGCTGTGTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTATTGATGGCTCATTTTGGCATAGGGGAGCAGTTTCAAATAAATGAGGGCCATGCTAGAGCAAAACTTGTTGTGGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

877

Amino Acids

99.73

Weight (kDa)

6.32

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 109 - 485 3.3e-98 Domain of unknown function (DUF4220)
DUF594 PF04578 807 - 857 3.4e-22 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1184
AccB7I CCANNNNNTGG 2 cut(s) 306, 2219
AccII CGCG 1 cut(s) 998
AccIII TCCGGA 1 cut(s) 225
AciI CCGC 2 cut(s) 124, 998
AclWI GGATC 4 cut(s) 79, 106, 1554, 1643
AcoI YGGCCR 3 cut(s) 1677, 2022, 2397
AcsI RAATTY 1 cut(s) 1476
AcuI CTGAAG 2 cut(s) 554, 2251
AcvI CACGTG 1 cut(s) 650
AfaI GTAC 5 cut(s) 335, 783, 867, 1741, 2211
AfiI CCNNNNNNNGG 3 cut(s) 306, 710, 2219
AflII CTTAAG 4 cut(s) 241, 695, 1310, 1460
AflIII ACRYGT 3 cut(s) 647, 2166, 2243
AgsI TTSAA 9 cut(s) 682, 833, 898, 988, 1441, 1941, 1950, 2419, 2585
AjiI CACGTC 1 cut(s) 2051
AjnI CCWGG 4 cut(s) 540, 809, 1194, 2212
AjuI GAANNNNNNNTTGG 2 cut(s) 2249, 2281
AleI CACNNNNGTG 2 cut(s) 462, 1037
Alw21I GWGCWC 5 cut(s) 110, 131, 1008, 1096, 2068
Alw44I GTGCAC 3 cut(s) 106, 1092, 2064
AlwI GGATC 4 cut(s) 79, 106, 1554, 1643
AlwNI CAGNNNCTG 1 cut(s) 554
Aor13HI TCCGGA 1 cut(s) 225
AoxI GGCC 9 cut(s) 3, 414, 529, 1192, 1677, 2016, 2022, 2397, 2596
ApaLI GTGCAC 3 cut(s) 106, 1092, 2064
ApeKI GCWGC 9 cut(s) 353, 798, 1280, 1283, 1994, 2090, 2188, 2407, 2474
ApoI RAATTY 1 cut(s) 1476
AseI ATTAAT 2 cut(s) 374, 992
AspS9I GGNCC 4 cut(s) 467, 713, 1572, 2596
AsuC2I CCSGG 1 cut(s) 1076
AsuHPI GGTGA 6 cut(s) 404, 851, 1024, 1633, 1697, 1771
AvaII GGWCC 3 cut(s) 467, 713, 1572
BaeGI GKGCMC 3 cut(s) 110, 1096, 2068
BalI TGGCCA 2 cut(s) 2024, 2399
BanI GGYRCC 1 cut(s) 1184
BanII GRGCYC 2 cut(s) 131, 1008
BbrPI CACGTG 1 cut(s) 650
BbsI GAAGAC 1 cut(s) 2196
Bbv12I GWGCWC 5 cut(s) 110, 131, 1008, 1096, 2068
BbvI GCAGC 9 cut(s) 340, 810, 1267, 1270, 2006, 2077, 2200, 2394, 2461
BccI CCATC 4 cut(s) 485, 1314, 1410, 2548
BceAI ACGGC 3 cut(s) 137, 933, 2003
BciT130I CCWGG 4 cut(s) 542, 811, 1196, 2214
BciVI GTATCC 2 cut(s) 588, 1058
BclI TGATCA 2 cut(s) 1351, 1761
BcnI CCSGG 1 cut(s) 1076
BfmI CTRYAG 4 cut(s) 1080, 1101, 2408, 2475
BfrI CTTAAG 4 cut(s) 241, 695, 1310, 1460
BfuI GTATCC 2 cut(s) 588, 1058
BglII AGATCT 1 cut(s) 913
BisI GCNGC 9 cut(s) 354, 799, 1281, 1284, 1995, 2091, 2189, 2408, 2475
BlsI GCNGC 9 cut(s) 355, 800, 1282, 1285, 1996, 2092, 2190, 2409, 2476
BmcAI AGTACT 1 cut(s) 1741
Bme1390I CCNGG 5 cut(s) 542, 811, 1076, 1196, 2214
Bme18I GGWCC 3 cut(s) 467, 713, 1572
BmgBI CACGTC 1 cut(s) 2051
BmgT120I GGNCC 4 cut(s) 467, 713, 1572, 2596
BmiI GGNNCC 2 cut(s) 159, 1186
BmrFI CCNGG 5 cut(s) 542, 811, 1076, 1196, 2214
BmrI ACTGGG 1 cut(s) 1699
BmsI GCATC 5 cut(s) 39, 697, 748, 1855, 2374
BmuI ACTGGG 1 cut(s) 1699
BpiI GAAGAC 1 cut(s) 2196
BplI GAGNNNNNCTC 2 cut(s) 956, 988
BpmI CTGGAG 2 cut(s) 1917, 2310
Bpu10I CCTNAGC 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 1076
BsaAI YACGTR 2 cut(s) 650, 2244
BsaJI CCNNGG 5 cut(s) 315, 388, 460, 1035, 2019
BsaWI WCCGGW 1 cut(s) 225
BsaXI ACNNNNNCTCC 2 cut(s) 2517, 2547
Bsc4I CCNNNNNNNGG 3 cut(s) 306, 710, 2219
Bse1I ACTGG 2 cut(s) 417, 1694
Bse3DI GCAATG 4 cut(s) 251, 855, 1824, 2482
BseAI TCCGGA 1 cut(s) 225
BseBI CCWGG 4 cut(s) 542, 811, 1196, 2214
BseDI CCNNGG 5 cut(s) 315, 388, 460, 1035, 2019
BseGI GGATG 2 cut(s) 1566, 1858
BseLI CCNNNNNNNGG 3 cut(s) 306, 710, 2219
BseMI GCAATG 4 cut(s) 251, 855, 1824, 2482
BseMII CTCAG 2 cut(s) 987, 1824
BseNI ACTGG 2 cut(s) 417, 1694
BseRI GAGGAG 1 cut(s) 736
BseSI GKGCMC 3 cut(s) 110, 1096, 2068
BseXI GCAGC 9 cut(s) 340, 810, 1267, 1270, 2006, 2077, 2200, 2394, 2461
BseYI CCCAGC 1 cut(s) 762
Bsh1236I CGCG 1 cut(s) 998
BshFI GGCC 9 cut(s) 5, 416, 531, 1194, 1679, 2018, 2024, 2399, 2598
BshNI GGYRCC 1 cut(s) 1184
BsiHKAI GWGCWC 5 cut(s) 110, 131, 1008, 1096, 2068
BsiSI CCGG 2 cut(s) 226, 1075
BslFI GGGAC 2 cut(s) 915, 1748
BslI CCNNNNNNNGG 3 cut(s) 306, 710, 2219
BsmFI GGGAC 2 cut(s) 915, 1748
BsmI GAATGC 3 cut(s) 1051, 1436, 1809
BsnI GGCC 9 cut(s) 5, 416, 531, 1194, 1679, 2018, 2024, 2399, 2598
Bsp1286I GDGCHC 5 cut(s) 110, 131, 1008, 1096, 2068
Bsp13I TCCGGA 1 cut(s) 225
Bsp143I GATC 9 cut(s) 71, 98, 421, 913, 1316, 1351, 1546, 1648, 1761
BspACI CCGC 2 cut(s) 124, 998
BspANI GGCC 9 cut(s) 5, 416, 531, 1194, 1679, 2018, 2024, 2399, 2598
BspCNI CTCAG 2 cut(s) 986, 1825
BspEI TCCGGA 1 cut(s) 225
BspFNI CGCG 1 cut(s) 998
BspHI TCATGA 1 cut(s) 931
BspLI GGNNCC 2 cut(s) 159, 1186
BspMAI CTGCAG 2 cut(s) 2412, 2479
BspPI GGATC 4 cut(s) 79, 106, 1554, 1643
BspT107I GGYRCC 1 cut(s) 1184
BspTI CTTAAG 4 cut(s) 241, 695, 1310, 1460
BsrDI GCAATG 4 cut(s) 251, 855, 1824, 2482
BsrI ACTGG 2 cut(s) 417, 1694
BssECI CCNNGG 5 cut(s) 315, 388, 460, 1035, 2019
BssMI GATC 9 cut(s) 71, 98, 421, 913, 1316, 1351, 1546, 1648, 1761
BssT1I CCWWGG 4 cut(s) 315, 388, 460, 1035
Bst2UI CCWGG 4 cut(s) 542, 811, 1196, 2214
Bst4CI ACNGT 9 cut(s) 55, 194, 939, 1240, 1382, 1739, 1744, 2147, 2157
Bst6I CTCTTC 2 cut(s) 569, 1034
BstAFI CTTAAG 4 cut(s) 241, 695, 1310, 1460
BstBAI YACGTR 2 cut(s) 650, 2244
BstC8I GCNNGC 4 cut(s) 667, 1580, 1641, 2389
BstDEI CTNAG 4 cut(s) 515, 973, 1056, 1833
BstDSI CCRYGG 1 cut(s) 2019
BstF5I GGATG 2 cut(s) 1566, 1858
BstFNI CGCG 1 cut(s) 998
BstKTI GATC 9 cut(s) 74, 101, 424, 916, 1319, 1354, 1549, 1651, 1764
BstMBI GATC 9 cut(s) 71, 98, 421, 913, 1316, 1351, 1546, 1648, 1761
BstMWI GCNNNNNNNGC 7 cut(s) 350, 362, 700, 1057, 1091, 1901, 2090
BstNI CCWGG 4 cut(s) 542, 811, 1196, 2214
BstNSI RCATGY 3 cut(s) 875, 2170, 2391
BstSCI CCNGG 5 cut(s) 540, 809, 1074, 1194, 2212
BstSFI CTRYAG 4 cut(s) 1080, 1101, 2408, 2475
BstSLI GKGCMC 3 cut(s) 110, 1096, 2068
BstUI CGCG 1 cut(s) 998
BstV1I GCAGC 9 cut(s) 340, 810, 1267, 1270, 2006, 2077, 2200, 2394, 2461
BstV2I GAAGAC 1 cut(s) 2196
BstX2I RGATCY 3 cut(s) 71, 913, 1546
BstXI CCANNNNNNTGG 1 cut(s) 1195
BstYI RGATCY 3 cut(s) 71, 913, 1546
BsuI GTATCC 2 cut(s) 588, 1058
BsuRI GGCC 9 cut(s) 5, 416, 531, 1194, 1679, 2018, 2024, 2399, 2598
BtgI CCRYGG 1 cut(s) 2019
BtrI CACGTC 1 cut(s) 2051
BtsCI GGATG 2 cut(s) 1566, 1858
BtsI GCAGTG 4 cut(s) 110, 480, 867, 1354
BtsIMutI CAGTG 8 cut(s) 110, 199, 424, 480, 867, 1354, 1641, 2153
Cac8I GCNNGC 4 cut(s) 667, 1580, 1641, 2389
CaiI CAGNNNCTG 1 cut(s) 554
CciI TCATGA 1 cut(s) 931
Cfr13I GGNCC 4 cut(s) 467, 713, 1572, 2596
Csp6I GTAC 5 cut(s) 334, 782, 866, 1740, 2210
CviQI GTAC 5 cut(s) 334, 782, 866, 1740, 2210
DdeI CTNAG 4 cut(s) 515, 973, 1056, 1833
DpnI GATC 9 cut(s) 73, 100, 423, 915, 1318, 1353, 1548, 1650, 1763
DpnII GATC 9 cut(s) 71, 98, 421, 913, 1316, 1351, 1546, 1648, 1761
DraI TTTAAA 1 cut(s) 1615
EaeI YGGCCR 3 cut(s) 1677, 2022, 2397
Eam1104I CTCTTC 2 cut(s) 569, 1034
EarI CTCTTC 2 cut(s) 569, 1034
EciI GGCGGA 1 cut(s) 139
Ecl136II GAGCTC 2 cut(s) 129, 1006
Eco130I CCWWGG 4 cut(s) 315, 388, 460, 1035
Eco147I AGGCCT 1 cut(s) 1194
Eco24I GRGCYC 2 cut(s) 131, 1008
Eco47I GGWCC 3 cut(s) 467, 713, 1572
Eco53kI GAGCTC 2 cut(s) 129, 1006
Eco57I CTGAAG 2 cut(s) 554, 2251
Eco72I CACGTG 1 cut(s) 650
EcoICRI GAGCTC 2 cut(s) 129, 1006
EcoRII CCWGG 4 cut(s) 540, 809, 1194, 2212
EcoT14I CCWWGG 4 cut(s) 315, 388, 460, 1035
EcoT22I ATGCAT 2 cut(s) 2389, 2393
EcoT38I GRGCYC 2 cut(s) 131, 1008
ErhI CCWWGG 4 cut(s) 315, 388, 460, 1035
FaqI GGGAC 2 cut(s) 915, 1748
FbaI TGATCA 2 cut(s) 1351, 1761
Fnu4HI GCNGC 9 cut(s) 354, 799, 1281, 1284, 1995, 2091, 2189, 2408, 2475
FokI GGATG 2 cut(s) 1573, 1865
FriOI GRGCYC 2 cut(s) 131, 1008
Fsp4HI GCNGC 9 cut(s) 354, 799, 1281, 1284, 1995, 2091, 2189, 2408, 2475
GluI GCNGC 9 cut(s) 354, 799, 1281, 1284, 1995, 2091, 2189, 2408, 2475
GsaI CCCAGC 1 cut(s) 766
GsuI CTGGAG 2 cut(s) 1917, 2310
HaeIII GGCC 9 cut(s) 5, 416, 531, 1194, 1679, 2018, 2024, 2399, 2598
HapII CCGG 2 cut(s) 226, 1075
HindIII AAGCTT 3 cut(s) 667, 728, 981
HpaII CCGG 2 cut(s) 226, 1075
HphI GGTGA 6 cut(s) 404, 851, 1024, 1633, 1697, 1771
Hpy166II GTNNAC 6 cut(s) 108, 1094, 1329, 1392, 1747, 2066
Hpy8I GTNNAC 6 cut(s) 108, 1094, 1329, 1392, 1747, 2066
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 3 cut(s) 578, 2268, 2413
HpyCH4III ACNGT 9 cut(s) 55, 194, 939, 1240, 1382, 1739, 1744, 2147, 2157
HpyCH4IV ACGT 5 cut(s) 86, 649, 1515, 2050, 2243
HpyF10VI GCNNNNNNNGC 7 cut(s) 350, 362, 700, 1057, 1091, 1901, 2090
HpyF3I CTNAG 4 cut(s) 515, 973, 1056, 1833
HpySE526I ACGT 5 cut(s) 86, 649, 1515, 2050, 2243
Kpn2I TCCGGA 1 cut(s) 225
Ksp22I TGATCA 2 cut(s) 1351, 1761
Kzo9I GATC 9 cut(s) 71, 98, 421, 913, 1316, 1351, 1546, 1648, 1761
LmnI GCTCC 4 cut(s) 126, 157, 228, 2575
Lsp1109I GCAGC 9 cut(s) 340, 810, 1267, 1270, 2006, 2077, 2200, 2394, 2461
LweI GCATC 5 cut(s) 39, 697, 748, 1855, 2374
MaeII ACGT 5 cut(s) 86, 649, 1515, 2050, 2243
MaeIII GTNAC 9 cut(s) 55, 175, 392, 453, 544, 573, 1240, 1685, 2533
MalI GATC 9 cut(s) 73, 100, 423, 915, 1318, 1353, 1548, 1650, 1763
MboI GATC 9 cut(s) 71, 98, 421, 913, 1316, 1351, 1546, 1648, 1761
MfeI CAATTG 1 cut(s) 2507
MflI RGATCY 3 cut(s) 71, 913, 1546
MhlI GDGCHC 5 cut(s) 110, 131, 1008, 1096, 2068
MlsI TGGCCA 2 cut(s) 2024, 2399
MluNI TGGCCA 2 cut(s) 2024, 2399
MlyI GAGTC 5 cut(s) 32, 433, 1262, 1351, 1708
MmeI TCCRAC 3 cut(s) 119, 349, 794
Mox20I TGGCCA 2 cut(s) 2024, 2399
Mph1103I ATGCAT 2 cut(s) 2389, 2393
MroI TCCGGA 1 cut(s) 225
MscI TGGCCA 2 cut(s) 2024, 2399
MslI CAYNNNNRTG 4 cut(s) 462, 860, 1037, 1149
Msp20I TGGCCA 2 cut(s) 2024, 2399
MspCI CTTAAG 4 cut(s) 241, 695, 1310, 1460
MspI CCGG 2 cut(s) 226, 1075
MspR9I CCNGG 5 cut(s) 542, 811, 1076, 1196, 2214
MunI CAATTG 1 cut(s) 2507
Mva1269I GAATGC 3 cut(s) 1051, 1436, 1809
MvaI CCWGG 4 cut(s) 542, 811, 1196, 2214
MvnI CGCG 1 cut(s) 998
MwoI GCNNNNNNNGC 7 cut(s) 350, 362, 700, 1057, 1091, 1901, 2090
NciI CCSGG 1 cut(s) 1076
NdeII GATC 9 cut(s) 71, 98, 421, 913, 1316, 1351, 1546, 1648, 1761
NlaIV GGNNCC 2 cut(s) 159, 1186
NmuCI GTSAC 5 cut(s) 55, 392, 573, 1685, 2533
NsiI ATGCAT 2 cut(s) 2389, 2393
NspI RCATGY 3 cut(s) 875, 2170, 2391
OliI CACNNNNGTG 2 cut(s) 462, 1037
PaeI GCATGC 1 cut(s) 2391
PagI TCATGA 1 cut(s) 931
PceI AGGCCT 1 cut(s) 1194
PciI ACATGT 1 cut(s) 2166
PctI GAATGC 3 cut(s) 1051, 1436, 1809
PflFI GACNNNGTC 1 cut(s) 2155
PflMI CCANNNNNTGG 2 cut(s) 306, 2219
PfoI TCCNGGA 1 cut(s) 809
PkrI GCNGC 9 cut(s) 355, 800, 1282, 1285, 1996, 2092, 2190, 2409, 2476
PleI GAGTC 5 cut(s) 31, 433, 1262, 1351, 1708
PmaCI CACGTG 1 cut(s) 650
PmlI CACGTG 1 cut(s) 650
PpsI GAGTC 5 cut(s) 31, 433, 1262, 1351, 1708
Ppu21I YACGTR 2 cut(s) 650, 2244
PscI ACATGT 1 cut(s) 2166
PshBI ATTAAT 2 cut(s) 374, 992
Psp124BI GAGCTC 2 cut(s) 131, 1008
Psp6I CCWGG 4 cut(s) 540, 809, 1194, 2212
PspCI CACGTG 1 cut(s) 650
PspFI CCCAGC 1 cut(s) 762
PspGI CCWGG 4 cut(s) 540, 809, 1194, 2212
PspN4I GGNNCC 2 cut(s) 159, 1186
PspPI GGNCC 4 cut(s) 467, 713, 1572, 2596
PstI CTGCAG 2 cut(s) 2412, 2479
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 3 cut(s) 71, 913, 1546
PsyI GACNNNGTC 1 cut(s) 2155
RsaI GTAC 5 cut(s) 335, 783, 867, 1741, 2211
RsaNI GTAC 5 cut(s) 334, 782, 866, 1740, 2210
RseI CAYNNNNRTG 4 cut(s) 462, 860, 1037, 1149
SacI GAGCTC 2 cut(s) 131, 1008
SatI GCNGC 9 cut(s) 354, 799, 1281, 1284, 1995, 2091, 2189, 2408, 2475
Sau3AI GATC 9 cut(s) 71, 98, 421, 913, 1316, 1351, 1546, 1648, 1761
Sau96I GGNCC 4 cut(s) 467, 713, 1572, 2596
ScaI AGTACT 1 cut(s) 1741
SchI GAGTC 5 cut(s) 32, 433, 1262, 1351, 1708
ScrFI CCNGG 5 cut(s) 542, 811, 1076, 1196, 2214
SduI GDGCHC 5 cut(s) 110, 131, 1008, 1096, 2068
SfaNI GCATC 5 cut(s) 39, 697, 748, 1855, 2374
SfcI CTRYAG 4 cut(s) 1080, 1101, 2408, 2475
SinI GGWCC 3 cut(s) 467, 713, 1572
SmiMI CAYNNNNRTG 4 cut(s) 462, 860, 1037, 1149
SmlI CTYRAG 4 cut(s) 241, 695, 1310, 1460
SmoI CTYRAG 4 cut(s) 241, 695, 1310, 1460
SphI GCATGC 1 cut(s) 2391
SseBI AGGCCT 1 cut(s) 1194
SsiI CCGC 2 cut(s) 124, 998
SstI GAGCTC 2 cut(s) 131, 1008
StuI AGGCCT 1 cut(s) 1194
StyD4I CCNGG 5 cut(s) 540, 809, 1074, 1194, 2212
StyI CCWWGG 4 cut(s) 315, 388, 460, 1035
TaaI ACNGT 9 cut(s) 55, 194, 939, 1240, 1382, 1739, 1744, 2147, 2157
TaiI ACGT 5 cut(s) 89, 652, 1518, 2053, 2246
TaqI TCGA 1 cut(s) 1552
TatI WGTACW 2 cut(s) 781, 1739
TscAI CASTG 8 cut(s) 110, 199, 424, 487, 867, 1354, 1641, 2160
TseFI GTSAC 5 cut(s) 55, 392, 573, 1685, 2533
TseI GCWGC 9 cut(s) 353, 798, 1280, 1283, 1994, 2090, 2188, 2407, 2474
Tsp45I GTSAC 5 cut(s) 55, 392, 573, 1685, 2533
TspDTI ATGAA 8 cut(s) 920, 948, 1035, 1191, 1204, 1437, 1597, 2091
TspGWI ACGGA 1 cut(s) 1151
TspRI CASTG 8 cut(s) 110, 199, 424, 487, 867, 1354, 1641, 2160
Tth111I GACNNNGTC 1 cut(s) 2155
Van91I CCANNNNNTGG 2 cut(s) 306, 2219
Vha464I CTTAAG 4 cut(s) 241, 695, 1310, 1460
VneI GTGCAC 3 cut(s) 106, 1092, 2064
VpaK11BI GGWCC 3 cut(s) 467, 713, 1572
VspI ATTAAT 2 cut(s) 374, 992
XapI RAATTY 1 cut(s) 1476
XbaI TCTAGA 1 cut(s) 1969
XceI RCATGY 3 cut(s) 875, 2170, 2391
XcmI CCANNNNNNNNNTGG 2 cut(s) 424, 1042
ZrmI AGTACT 1 cut(s) 1741
Zsp2I ATGCAT 2 cut(s) 2389, 2393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.