Rmu_sc0000570.1_g000032

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000570.1
OG0000662
Physical Location & Seq
Forward (+)
156777 .. 159884
3108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000570.1_g000032.1.cds

Sequence Viewer

Length: 2346 bp
atggtttctcccattcctaccagtgtcaagaatatatgggaaaagtggaatctccggggttttgtactcctcagcctctcattgcaaactattttaattcttggtgcaccctttcgaaagcgatcacctagcttatttgtaaccatggttatctggtcatcttacttgcttgcagactgggcagctaactttgcaattggcttaattgcaaacagccagggcgacaatccatttattccaaaccccaacaacactggtcattttaatagcgatcttctggcattttgggcgccctttcttctgttacaccttggtggtccagacacaattacagcatttgctcttgaggataacacattgtggctaagacacttgtttgggcttgtgttccaggttatagctgcggtttatgtcttcattcaatcatttcccaaaaacaagctgtggttgccgactgttctcctatttgtagcaggaatcatcaagtatgccgagcgcacacgtgctctgtatcttgcaagcttggataattttaaggagtccatgattaagaaacctgatcctggtcccaattatgcaaagctgatggaggaatactcttcgaaaaaagatgccaagcttccaactcatatagagttgacagcggagcgcagcaaagattccaggactactacttatgtggtggaccctggagatatggaacataatattgcactggttcgccatgcttttcacttctttaacatcttcaagggtcttattgtggatctcattttcagctttcatgaacgatttgagagccgtgtgtttttccatgacagaacagcagaggatgctttcaagttggttgcaattgagcttaatttcatatatgaagctctattcaccaaggtggtggttgtgcattctaagaagggatacatttgccgagctatatcctttagtgcagtttgcacagctcttgtttgcttccaaaaaatagagaaacatgatttccatgggtttgatgttggaattacctatattttgctttttggtgccttggccttggattcaatagctgtgttcatgcttatattctctgattggactgttgctgctgtgaagaagtctagccaggattcttgtcttgccaatattctcaaaaggtacatcactctcaagcaatcttggtggttctttgccgaaaatgcaaatccatatttggagaggtgtaggcaacttctatttcgaaggtggtacgaatctgtctccatatttaacttgatacattattccctcaaggaatgtccaaaaccgagtcctaatatttttgattacttctgcatggcctgcttagaaatcatctacttgatgggtcttaaggatcttcgagataagatcaaatataggaggagcaagcgactcgaaaaagagctatggaaattcatttttgatcagttgaaaagtaagtccatgctcgcagatgatcctgaaactgccaggagaataagttcggccagaggtgactggattctacaggatagtgactggaataactataaagattgtcgtaacttgctccgttatgttgttgatgttgaatttgatcagagcattctactgtggcacattgctactgagttttgttataatcttgacatggatctaaaagttcaggagaaggaaccatacaaggctgtagctgttcagactgcaattgaacattgtaagatcttgtctgattacatggtatacctcttagttatgcagcctactatgatgtctactgtggcaggaattggacaaataagattccgcgatacatgtgcagaagccataaagttcttcagcagaagggaactaggaccaaaagtcactccacaagaggcatgtgagagaattctcgaggtaaatacagatgttcggccggttgctgttaaaggggatcgaagtaaatccgtgttgtttgatgcatctattctggcgaaagaactgatgactttggatgaaaagaaatggaaactcatgagtgaagtgtgggtggaactactagcatatgctgcaggtcattgcagagcaaatgaccatgtccaattgctgagcaaaggtggagagcttgtcacttttgtttggactgcttcttcctccaccgctagcgaaatcgtggaggctcaaggccacattctaagggccaccagccggaaggacagccacagcaaggtttgcaccaccaaaggcccctgggatcgcagtatcaggctcgtcgcccacaccgccattcaattctacgacatgcaagaccgactcggatacgaccagcctagcaaggtcgtcgattggccgcgcaggacacgcagaacgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

781

Amino Acids

89.98

Weight (kDa)

8.77

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1632
Acc36I ACCTGC 1 cut(s) 2036
AccB1I GGYRCC 2 cut(s) 289, 1037
AccI GTMKAC 2 cut(s) 1734, 1766
AccII CGCG 2 cut(s) 1800, 2326
AciI CCGC 6 cut(s) 404, 644, 1798, 2133, 2256, 2324
AclWI GGATC 7 cut(s) 554, 774, 1374, 1463, 1653, 1935, 2235
AcoI YGGCCR 3 cut(s) 1497, 1907, 2321
AcsI RAATTY 3 cut(s) 1424, 1583, 1881
AcuI CTGAAG 1 cut(s) 1813
AcvI CACGTG 1 cut(s) 503
AcyI GRCGYC 1 cut(s) 290
AdeI CACNNNGTG 1 cut(s) 360
AfaI GTAC 3 cut(s) 66, 1151, 1241
AfiI CCNNNNNNNGG 3 cut(s) 563, 2181, 2200
AflII CTTAAG 1 cut(s) 1361
AflIII ACRYGT 2 cut(s) 500, 1805
AgsI TTSAA 8 cut(s) 422, 751, 841, 1056, 1444, 1583, 1703, 2264
AhdI GACNNNNNGTC 1 cut(s) 1853
AjnI CCWGG 8 cut(s) 216, 390, 562, 662, 688, 1116, 1481, 2222
AleI CACNNNNGTG 2 cut(s) 312, 890
AloI GAACNNNNNNTCC 2 cut(s) 1477, 1509
Alw21I GWGCWC 2 cut(s) 109, 508
Alw26I GTCTC 1 cut(s) 1255
Alw44I GTGCAC 1 cut(s) 105
AlwI GGATC 7 cut(s) 554, 774, 1374, 1463, 1653, 1935, 2235
Ama87I CYCGRG 1 cut(s) 1886
AoxI GGCC 8 cut(s) 1044, 1329, 1497, 1907, 2158, 2172, 2218, 2321
ApaLI GTGCAC 1 cut(s) 105
ApeKI GCWGC 6 cut(s) 182, 401, 651, 1097, 1750, 2042
ApoI RAATTY 3 cut(s) 1424, 1583, 1881
Asp700I GAANNNNTTC 1 cut(s) 1492
AspLEI GCGC 4 cut(s) 292, 498, 651, 2328
AspS9I GGNCC 6 cut(s) 317, 566, 685, 1847, 2172, 2219
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 3 cut(s) 117, 877, 1517
AsuII TTCGAA 3 cut(s) 115, 602, 1231
AsuNHI GCTAGC 1 cut(s) 2135
AvaI CYCGRG 1 cut(s) 1886
AvaII GGWCC 4 cut(s) 317, 566, 685, 1847
BaeGI GKGCMC 1 cut(s) 109
BanI GGYRCC 2 cut(s) 289, 1037
BarI GAAGNNNNNNTAC 2 cut(s) 1655, 1687
BbrPI CACGTG 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 2 cut(s) 109, 508
BbvCI CCTCAGC 1 cut(s) 71
BbvI GCAGC 6 cut(s) 194, 388, 663, 1084, 1762, 2029
BccI CCATC 2 cut(s) 580, 1348
BceAI ACGGC 1 cut(s) 786
BcgI CGANNNNNNTGC 6 cut(s) 1387, 1421, 1790, 1824, 2296, 2330
BciT130I CCWGG 8 cut(s) 218, 392, 564, 664, 690, 1118, 1483, 2224
BciVI GTATCC 2 cut(s) 911, 2285
BclI TGATCA 2 cut(s) 1435, 1588
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 1255
BfaI CTAG 6 cut(s) 129, 1113, 1844, 2033, 2136, 2304
BfmI CTRYAG 3 cut(s) 1517, 1680, 2043
BfoI RGCGCY 1 cut(s) 293
BfrI CTTAAG 1 cut(s) 1361
BfuAI ACCTGC 1 cut(s) 2036
BfuI GTATCC 2 cut(s) 911, 2285
BglII AGATCT 1 cut(s) 1713
BisI GCNGC 7 cut(s) 183, 402, 652, 1098, 1751, 2043, 2324
BlpI GCTNAGC 1 cut(s) 2081
BlsI GCNGC 7 cut(s) 184, 403, 653, 1099, 1752, 2044, 2325
Bme1390I CCNGG 9 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483, 2224
Bme18I GGWCC 4 cut(s) 317, 566, 685, 1847
BmeRI GACNNNNNGTC 1 cut(s) 1853
BmeT110I CYCGRG 1 cut(s) 1886
BmgT120I GGNCC 6 cut(s) 317, 566, 685, 1847, 2172, 2219
BmiI GGNNCC 6 cut(s) 291, 568, 687, 1039, 1668, 2221
BmrFI CCNGG 9 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483, 2224
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 4 cut(s) 601, 823, 1942, 1964
BmtI GCTAGC 1 cut(s) 2139
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 406
BplI GAGNNNNNCTC 2 cut(s) 581, 613
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 1 cut(s) 71
Bpu1102I GCTNAGC 1 cut(s) 2081
Bpu14I TTCGAA 3 cut(s) 115, 602, 1231
BpuEI CTTGAG 4 cut(s) 365, 1145, 1265, 2139
BpuMI CCSGG 1 cut(s) 56
BsaAI YACGTR 1 cut(s) 503
BsaHI GRCGYC 1 cut(s) 290
BsaXI ACNNNNNCTCC 4 cut(s) 1477, 1507, 2085, 2115
Bsc4I CCNNNNNNNGG 3 cut(s) 563, 2181, 2200
Bse118I RCCGGY 1 cut(s) 1909
Bse1I ACTGG 6 cut(s) 21, 182, 259, 720, 1514, 1535
Bse3DI GCAATG 3 cut(s) 80, 1611, 2050
BseBI CCWGG 8 cut(s) 218, 392, 564, 664, 690, 1118, 1483, 2224
BseGI GGATG 2 cut(s) 838, 1993
BseLI CCNNNNNNNGG 3 cut(s) 563, 2181, 2200
BseMI GCAATG 3 cut(s) 80, 1611, 2050
BseMII CTCAG 3 cut(s) 85, 1611, 2072
BseNI ACTGG 6 cut(s) 21, 182, 259, 720, 1514, 1535
BseRI GAGGAG 2 cut(s) 59, 1408
BseSI GKGCMC 1 cut(s) 109
BseX3I CGGCCG 1 cut(s) 1907
BseXI GCAGC 6 cut(s) 194, 388, 663, 1084, 1762, 2029
BsgI GTGCAG 2 cut(s) 966, 1830
Bsh1236I CGCG 2 cut(s) 1800, 2326
Bsh1285I CGRYCG 1 cut(s) 1910
BshFI GGCC 8 cut(s) 1046, 1331, 1499, 1909, 2160, 2174, 2220, 2323
BshNI GGYRCC 2 cut(s) 289, 1037
BsiEI CGRYCG 1 cut(s) 1910
BsiHKAI GWGCWC 2 cut(s) 109, 508
BsiHKCI CYCGRG 1 cut(s) 1886
BsiSI CCGG 3 cut(s) 55, 1910, 2182
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 3 cut(s) 563, 2181, 2200
BsmAI GTCTC 1 cut(s) 1255
BsmFI GGGAC 1 cut(s) 552
BsmI GAATGC 2 cut(s) 904, 1596
BsnI GGCC 8 cut(s) 1046, 1331, 1499, 1909, 2160, 2174, 2220, 2323
BsoBI CYCGRG 1 cut(s) 1886
Bsp119I TTCGAA 3 cut(s) 115, 602, 1231
Bsp1286I GDGCHC 2 cut(s) 109, 508
Bsp1720I GCTNAGC 1 cut(s) 2081
Bsp19I CCATGG 2 cut(s) 144, 997
BspACI CCGC 6 cut(s) 404, 644, 1798, 2133, 2256, 2324
BspANI GGCC 8 cut(s) 1046, 1331, 1499, 1909, 2160, 2174, 2220, 2323
BspCNI CTCAG 3 cut(s) 84, 1612, 2073
BspFNI CGCG 2 cut(s) 1800, 2326
BspHI TCATGA 2 cut(s) 784, 2007
BspLI GGNNCC 6 cut(s) 291, 568, 687, 1039, 1668, 2221
BspMAI CTGCAG 1 cut(s) 2047
BspMI ACCTGC 1 cut(s) 2036
BspOI GCTAGC 1 cut(s) 2139
BspPI GGATC 7 cut(s) 554, 774, 1374, 1463, 1653, 1935, 2235
BspT104I TTCGAA 3 cut(s) 115, 602, 1231
BspT107I GGYRCC 2 cut(s) 289, 1037
BspTI CTTAAG 1 cut(s) 1361
BsrDI GCAATG 3 cut(s) 80, 1611, 2050
BsrFI RCCGGY 1 cut(s) 1909
BsrI ACTGG 6 cut(s) 21, 182, 259, 720, 1514, 1535
BssAI RCCGGY 1 cut(s) 1909
BssNAI GTATAC 1 cut(s) 1735
BssNI GRCGYC 1 cut(s) 290
BssT1I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
Bst1107I GTATAC 1 cut(s) 1735
Bst2UI CCWGG 8 cut(s) 218, 392, 564, 664, 690, 1118, 1483, 2224
Bst4CI ACNGT 4 cut(s) 457, 1093, 1605, 1771
Bst6I CTCTTC 1 cut(s) 604
BstACI GRCGYC 1 cut(s) 290
BstAFI CTTAAG 1 cut(s) 1361
BstAPI GCANNNNNTGC 4 cut(s) 833, 1332, 2042, 2205
BstBAI YACGTR 1 cut(s) 503
BstBI TTCGAA 3 cut(s) 115, 602, 1231
BstC8I GCNNGC 6 cut(s) 171, 520, 1333, 1400, 1461, 2137
BstDEI CTNAG 8 cut(s) 71, 365, 909, 1336, 1620, 1741, 2081, 2168
BstDSI CCRYGG 2 cut(s) 144, 997
BstF5I GGATG 2 cut(s) 838, 1993
BstFNI CGCG 2 cut(s) 1800, 2326
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 4 cut(s) 292, 498, 651, 2328
BstMAI GTCTC 1 cut(s) 1255
BstMCI CGRYCG 1 cut(s) 1910
BstNI CCWGG 8 cut(s) 218, 392, 564, 664, 690, 1118, 1483, 2224
BstNSI RCATGY 3 cut(s) 1809, 1875, 2278
BstSCI CCNGG 9 cut(s) 54, 216, 390, 562, 662, 688, 1116, 1481, 2222
BstSFI CTRYAG 3 cut(s) 1517, 1680, 2043
BstSLI GKGCMC 1 cut(s) 109
BstUI CGCG 2 cut(s) 1800, 2326
BstV1I GCAGC 6 cut(s) 194, 388, 663, 1084, 1762, 2029
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 4 cut(s) 766, 1366, 1645, 1713
BstXI CCANNNNNNTGG 1 cut(s) 895
BstYI RGATCY 4 cut(s) 766, 1366, 1645, 1713
BstZ17I GTATAC 1 cut(s) 1735
BstZI CGGCCG 1 cut(s) 1907
BsuI GTATCC 2 cut(s) 911, 2285
BsuRI GGCC 8 cut(s) 1046, 1331, 1499, 1909, 2160, 2174, 2220, 2323
BtgI CCRYGG 2 cut(s) 144, 997
BtsCI GGATG 2 cut(s) 838, 1993
BtsIMutI CAGTG 3 cut(s) 28, 252, 713
BveI ACCTGC 1 cut(s) 2036
Cac8I GCNNGC 6 cut(s) 171, 520, 1333, 1400, 1461, 2137
CciI TCATGA 2 cut(s) 784, 2007
CfoI GCGC 4 cut(s) 292, 498, 651, 2328
Cfr10I RCCGGY 1 cut(s) 1909
Cfr13I GGNCC 6 cut(s) 317, 566, 685, 1847, 2172, 2219
Csp6I GTAC 3 cut(s) 65, 1150, 1240
CspCI CAANNNNNGTGG 2 cut(s) 1154, 1189
CviQI GTAC 3 cut(s) 65, 1150, 1240
DdeI CTNAG 8 cut(s) 71, 365, 909, 1336, 1620, 1741, 2081, 2168
DinI GGCGCC 1 cut(s) 291
DraIII CACNNNGTG 1 cut(s) 360
DriI GACNNNNNGTC 1 cut(s) 1853
EaeI YGGCCR 3 cut(s) 1497, 1907, 2321
EagI CGGCCG 1 cut(s) 1907
Eam1104I CTCTTC 1 cut(s) 604
Eam1105I GACNNNNNGTC 1 cut(s) 1853
EarI CTCTTC 1 cut(s) 604
EclXI CGGCCG 1 cut(s) 1907
Eco130I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
Eco47I GGWCC 4 cut(s) 317, 566, 685, 1847
Eco52I CGGCCG 1 cut(s) 1907
Eco57I CTGAAG 1 cut(s) 1813
Eco72I CACGTG 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 1886
EcoO109I RGGNCCY 1 cut(s) 2219
EcoRI GAATTC 1 cut(s) 1881
EcoRII CCWGG 8 cut(s) 216, 390, 562, 662, 688, 1116, 1481, 2222
EcoT14I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
EcoT22I ATGCAT 1 cut(s) 1957
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
FaqI GGGAC 1 cut(s) 552
FauNDI CATATG 1 cut(s) 2038
FbaI TGATCA 2 cut(s) 1435, 1588
FblI GTMKAC 2 cut(s) 1734, 1766
Fnu4HI GCNGC 7 cut(s) 183, 402, 652, 1098, 1751, 2043, 2324
FokI GGATG 2 cut(s) 845, 2000
Fsp4HI GCNGC 7 cut(s) 183, 402, 652, 1098, 1751, 2043, 2324
FspBI CTAG 6 cut(s) 129, 1113, 1844, 2033, 2136, 2304
GlaI GCGC 4 cut(s) 291, 497, 650, 2327
GluI GCNGC 7 cut(s) 183, 402, 652, 1098, 1751, 2043, 2324
GsuI CTGGAG 1 cut(s) 711
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 8 cut(s) 1046, 1331, 1499, 1909, 2160, 2174, 2220, 2323
HapII CCGG 3 cut(s) 55, 1910, 2182
HhaI GCGC 4 cut(s) 292, 498, 651, 2328
Hin1I GRCGYC 1 cut(s) 290
Hin6I GCGC 4 cut(s) 290, 496, 649, 2326
HinP1I GCGC 4 cut(s) 290, 496, 649, 2326
HincII GTYRAC 1 cut(s) 639
HindII GTYRAC 1 cut(s) 639
HindIII AAGCTT 2 cut(s) 520, 617
HpaII CCGG 3 cut(s) 55, 1910, 2182
HphI GGTGA 3 cut(s) 117, 877, 1517
Hpy166II GTNNAC 5 cut(s) 107, 639, 685, 1735, 1767
Hpy188I TCNGA 5 cut(s) 1084, 1593, 1692, 1723, 2291
Hpy8I GTNNAC 5 cut(s) 107, 639, 685, 1735, 1767
Hpy99I CGWCG 2 cut(s) 2249, 2318
HpyAV CCTTC 5 cut(s) 907, 1227, 1657, 1830, 2179
HpyCH4III ACNGT 4 cut(s) 457, 1093, 1605, 1771
HpyCH4IV ACGT 2 cut(s) 502, 2342
HpyF3I CTNAG 8 cut(s) 71, 365, 909, 1336, 1620, 1741, 2081, 2168
HpySE526I ACGT 2 cut(s) 502, 2342
Hsp92I GRCGYC 1 cut(s) 290
HspAI GCGC 4 cut(s) 290, 496, 649, 2326
KasI GGCGCC 1 cut(s) 289
Ksp22I TGATCA 2 cut(s) 1435, 1588
LmnI GCTCC 3 cut(s) 646, 1395, 1566
Lsp1109I GCAGC 6 cut(s) 194, 388, 663, 1084, 1762, 2029
LweI GCATC 4 cut(s) 601, 823, 1942, 1964
MaeI CTAG 6 cut(s) 129, 1113, 1844, 2033, 2136, 2304
MaeII ACGT 2 cut(s) 502, 2342
MaeIII GTNAC 7 cut(s) 139, 303, 1505, 1526, 1553, 1855, 2101
MboII GAAGA 9 cut(s) 266, 290, 406, 591, 739, 1117, 1361, 1819, 2115
MfeI CAATTG 4 cut(s) 195, 852, 1698, 2075
MflI RGATCY 4 cut(s) 766, 1366, 1645, 1713
MhlI GDGCHC 2 cut(s) 109, 508
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 4 cut(s) 548, 1309, 1398, 2280
MmeI TCCRAC 2 cut(s) 647, 991
Mph1103I ATGCAT 1 cut(s) 1957
MroXI GAANNNNTTC 1 cut(s) 1492
MslI CAYNNNNRTG 2 cut(s) 312, 890
MspA1I CMGCKG 1 cut(s) 644
MspCI CTTAAG 1 cut(s) 1361
MspI CCGG 3 cut(s) 55, 1910, 2182
MspR9I CCNGG 9 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483, 2224
MunI CAATTG 4 cut(s) 195, 852, 1698, 2075
Mva1269I GAATGC 2 cut(s) 904, 1596
MvaI CCWGG 8 cut(s) 218, 392, 564, 664, 690, 1118, 1483, 2224
MvnI CGCG 2 cut(s) 1800, 2326
NarI GGCGCC 1 cut(s) 290
NciI CCSGG 1 cut(s) 56
NcoI CCATGG 2 cut(s) 144, 997
NdeI CATATG 1 cut(s) 2038
NheI GCTAGC 1 cut(s) 2135
NlaIV GGNNCC 6 cut(s) 291, 568, 687, 1039, 1668, 2221
NmeAIII GCCGAG 2 cut(s) 517, 953
NmuCI GTSAC 4 cut(s) 1505, 1526, 1855, 2101
NsiI ATGCAT 1 cut(s) 1957
NspI RCATGY 3 cut(s) 1809, 1875, 2278
NspV TTCGAA 3 cut(s) 115, 602, 1231
OliI CACNNNNGTG 2 cut(s) 312, 890
PaeR7I CTCGAG 1 cut(s) 1886
PagI TCATGA 2 cut(s) 784, 2007
PasI CCCWGGG 1 cut(s) 2223
PciI ACATGT 1 cut(s) 1805
PctI GAATGC 2 cut(s) 904, 1596
PdmI GAANNNNTTC 1 cut(s) 1492
PfeI GAWTC 8 cut(s) 49, 477, 659, 1052, 1121, 1244, 1513, 1794
PflFI GACNNNGTC 1 cut(s) 2069
PfoI TCCNGGA 1 cut(s) 662
PkrI GCNGC 7 cut(s) 184, 403, 653, 1099, 1752, 2044, 2325
PleI GAGTC 4 cut(s) 547, 1308, 1398, 2280
PluTI GGCGCC 1 cut(s) 293
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 4 cut(s) 547, 1308, 1398, 2280
Ppu21I YACGTR 1 cut(s) 503
PscI ACATGT 1 cut(s) 1805
PsiI TTATAA 1 cut(s) 1632
Psp6I CCWGG 8 cut(s) 216, 390, 562, 662, 688, 1116, 1481, 2222
PspCI CACGTG 1 cut(s) 503
PspGI CCWGG 8 cut(s) 216, 390, 562, 662, 688, 1116, 1481, 2222
PspN4I GGNNCC 6 cut(s) 291, 568, 687, 1039, 1668, 2221
PspPI GGNCC 6 cut(s) 317, 566, 685, 1847, 2172, 2219
PstI CTGCAG 1 cut(s) 2047
PsuI RGATCY 4 cut(s) 766, 1366, 1645, 1713
PsyI GACNNNGTC 1 cut(s) 2069
RsaI GTAC 3 cut(s) 66, 1151, 1241
RsaNI GTAC 3 cut(s) 65, 1150, 1240
RseI CAYNNNNRTG 2 cut(s) 312, 890
SatI GCNGC 7 cut(s) 183, 402, 652, 1098, 1751, 2043, 2324
Sau96I GGNCC 6 cut(s) 317, 566, 685, 1847, 2172, 2219
SchI GAGTC 4 cut(s) 548, 1309, 1398, 2280
ScrFI CCNGG 9 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483, 2224
SduI GDGCHC 2 cut(s) 109, 508
SfaNI GCATC 4 cut(s) 601, 823, 1942, 1964
SfcI CTRYAG 3 cut(s) 1517, 1680, 2043
SfoI GGCGCC 1 cut(s) 291
Sfr274I CTCGAG 1 cut(s) 1886
SfuI TTCGAA 3 cut(s) 115, 602, 1231
SinI GGWCC 4 cut(s) 317, 566, 685, 1847
SlaI CTCGAG 1 cut(s) 1886
SmiMI CAYNNNNRTG 2 cut(s) 312, 890
SmlI CTYRAG 6 cut(s) 344, 1160, 1280, 1361, 1886, 2154
SmoI CTYRAG 6 cut(s) 344, 1160, 1280, 1361, 1886, 2154
SsiI CCGC 6 cut(s) 404, 644, 1798, 2133, 2256, 2324
SspDI GGCGCC 1 cut(s) 289
SspI AATATT 3 cut(s) 709, 1138, 1309
SspMI CTAG 6 cut(s) 129, 1113, 1844, 2033, 2136, 2304
StyD4I CCNGG 9 cut(s) 54, 216, 390, 562, 662, 688, 1116, 1481, 2222
StyI CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
TaaI ACNGT 4 cut(s) 457, 1093, 1605, 1771
TaiI ACGT 2 cut(s) 505, 2345
TaqI TCGA 8 cut(s) 115, 602, 1231, 1372, 1407, 1887, 1930, 2316
TaqII GACCGA 1 cut(s) 2298
TatI WGTACW 1 cut(s) 64
TauI GCSGC 1 cut(s) 2326
TfiI GAWTC 8 cut(s) 49, 477, 659, 1052, 1121, 1244, 1513, 1794
TscAI CASTG 3 cut(s) 28, 259, 720
TseFI GTSAC 4 cut(s) 1505, 1526, 1855, 2101
TseI GCWGC 6 cut(s) 182, 401, 651, 1097, 1750, 2042
Tsp45I GTSAC 4 cut(s) 1505, 1526, 1855, 2101
TspDTI ATGAA 8 cut(s) 406, 773, 801, 856, 888, 1057, 1417, 2004
TspGWI ACGGA 2 cut(s) 1553, 1930
TspRI CASTG 3 cut(s) 28, 259, 720
Tth111I GACNNNGTC 1 cut(s) 2069
Vha464I CTTAAG 1 cut(s) 1361
VneI GTGCAC 1 cut(s) 105
VpaK11BI GGWCC 4 cut(s) 317, 566, 685, 1847
XapI RAATTY 3 cut(s) 1424, 1583, 1881
XceI RCATGY 3 cut(s) 1809, 1875, 2278
XhoI CTCGAG 1 cut(s) 1886
XmiI GTMKAC 2 cut(s) 1734, 1766
XmnI GAANNNNTTC 1 cut(s) 1492
XspI CTAG 6 cut(s) 129, 1113, 1844, 2033, 2136, 2304
Zsp2I ATGCAT 1 cut(s) 1957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.