Rroxscaffold_2G00131110

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000662
Physical Location & Seq
Forward (+)
67659296 .. 67668685
9390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00131110.1

Sequence Viewer

Length: 2259 bp
ATGAGACAGGATATGGCTTCATTAGCCGCTCCTATTTCTAACAAGGTCAAGAAGATCTGGGATAAGTGGAATCTGCGGAGCTTCATCATATTAAGTCTCTCATTGCAAACCTTCTTAATTCTCTTTGCACCCTTGAGAAAGAGAACATCGAGCAAATGGGTAATCGTGCCTATCTGGTTAGCTTACTTGCTCGCTGACTGGGCTGCTAACTTTGCTATCGGACTAATTGCAAGCAGCCAAACGAACACTGATCAAGGTTCCCCAGAGAATGGTAATCTCTTGACATTCTGGGCTCCCTTTCTTCTTTTGCACCTTGGTGGTCCAGACACCATAATTGCAATTGCTATGGAAGACAATGCGTTGTGGCCAAGGCACTTGCTTGGTCTCATATTCCAGGTTGTAGCTGCAATGTACGTCTTCGGTATGCAGTTCTCTAACCAAAACAAGCTACTGTGGCCAACACTCTTGCTGTTTGTACCAGGACTTGTCAAGTACGGCGAGCGAACGCGTGCTCTATATCTTGCCAGCTTGGAAAGTCTCAAGGAAACCATACTTGCAATACCGGAACCTGGACCGGATTATGCAAAGTACATGGATGAGTACTCCTCAAGAAAAGCTGCCGGTCTTCCAATTACAATAATAAAAGAAGGAGGTAGCTCAGAGTCCAAGAATGTGACTTACCGGCTGGGAGAAGGAAAAATTGATAATATTACACTAGTGGTGGGTGCTCGTCACCTGTTTCAAATCTCCATGGGTCTTATTATAGATCTTGTGTTCGATTATCATCAGCGCAATGAAAGCCGTGCCTATTTCAATAAATTAGAATCAGGAGATGCTTTTACATCGATTGCGACTGAGCTCAATTTTATGTATGAAGCTCTGTACACCAAAGCTTATGTGGTACATTCTCGATGCGGATACTTGTTGAGAGCTACATCCTTCATCGCAGTTTGCATAGCCTTTGTTTTCTTCTATAGATTAGAGAAACATGGCTTTTACAGGTTTGATGTTGGAATTACTTATTCCTTGCTTTTTGGTGCCATAGCTCTTGATGCAATAGCTCTTTCAATGCTGATATTATCTGATTGGACGATTGCTGTTGTATACAACTTCTGTAAAGAGTTTTGTGTAGTCACTGATCTCATGAAGAAGTACCTGGAGTTTAAGAGATCATGGCGACCAACTACTACATCAAAGTTTCTGGAGCGGACCAGAAGGATTTTATTTCGAAGATGGTGTGAATCTGTCTCGGGTTTCAGCTTCATAGATTATTCTCTCCGAGAATGTGGAAGATCAGTAGTCCGACCTGCAATTTTGGACCAAATTGGCATTTACCGAAGGATCATCAGAATTTTGGACTACCTTTACCTTAGAATCATCATATTCCTGGGCCTCAAGTATCTTCGAGATGAGATGAAGTATAGATCCAGCAAGAGACTGAATGAGAATCTGTGGAATTTCATTTTTATTGAGTTGAAATTTAAATCCTCGGAAGCAGAAGCAAAAGAAAAAGCAGATGATGCTGATCACAAAATTTCTACAAAAATATGTTCTTCCCAAGGGGATTGGGTTCTCCGAGAAAGGTATGAGAAGCATCCGGAGTACAAGGACTTACAATCGTGCTTTGTTGATGTTACTTATGACCGACGCGTTCTGCTGTGGCATATTGCTACTGAGATGTGCTATTACAGAGTTGATACAGGCAATGTTGGTGAGCATGGTGAATTTAGTAAGACGTTGTCTGATTATATGCTATATCTCTTAATTATGCAGCCTGCTTTGATGTCCTCTGTAGCGGGAATCGGACAAAAGAGATTTAGAGATACGGTTGCAGAAGCCAAAAAATTCTTTAAAAAAAGGGGATTGGAATCCGATGTGAAACTAGGAGACGGAAAACTATGCGAGGCTTGTTACAAACTCCATGATGTAGATACAGAAGTTAAACCAGTCAATGTCAAAGGGAATAGAAGTAAATCTGTTTTGTTTGATGCATGTATTCTGGCCAAGCAGCTGATGAAATTCGGAGACTCGAAATGGGAATTAATAAGCTCCGTGTGGGTGGAATTGCTCTCATATGCTGCAAGTCATTGCAGAGCACATAGCCACGCGCAATTACTGACTAAAGGTGGAGAGCTTGTCAGTTTTGTTTGGTTACTGATGGCTCGCTTTGGCATTGAGGAGGACATCCAAGTACTGGAGGTCCAAGCAAGAGTAAAACTGATTATGGACAAGCACCCTGATGTATTCAAGAGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

752

Amino Acids

86.46

Weight (kDa)

9.03

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 59 - 424 7.4e-89 Domain of unknown function (DUF4220)
DUF594 PF04578 676 - 725 2.3e-20 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1315
AccB1I GGYRCC 1 cut(s) 1037
AccB7I CCANNNNNTGG 2 cut(s) 269, 2194
AccBSI CCGCTC 2 cut(s) 29, 1207
AccI GTMKAC 1 cut(s) 1104
AccII CGCG 3 cut(s) 508, 1650, 2108
AccIII TCCGGA 1 cut(s) 1597
AciI CCGC 5 cut(s) 27, 76, 915, 1207, 1796
AclWI GGATC 2 cut(s) 1349, 1419
AcoI YGGCCR 3 cut(s) 365, 455, 2001
AcsI RAATTY 7 cut(s) 1350, 1456, 1478, 1533, 1724, 1844, 2018
AfiI CCNNNNNNNGG 3 cut(s) 269, 569, 2194
AflIII ACRYGT 2 cut(s) 506, 1648
AgsI TTSAA 5 cut(s) 743, 814, 1068, 1477, 2248
AhlI ACTAGT 1 cut(s) 715
AjnI CCWGG 5 cut(s) 393, 478, 568, 1155, 1386
AleI CACNNNNGTG 1 cut(s) 315
Alw21I GWGCWC 4 cut(s) 514, 730, 861, 2098
Alw26I GTCTC 7 cut(s) 101, 389, 542, 1252, 1429, 1881, 2019
AlwI GGATC 2 cut(s) 1349, 1419
Ama87I CYCGRG 1 cut(s) 1249
Aor13HI TCCGGA 1 cut(s) 1597
AoxI GGCC 4 cut(s) 365, 455, 1390, 2001
ApeKI GCWGC 7 cut(s) 203, 234, 404, 617, 1771, 2008, 2078
ApoI RAATTY 7 cut(s) 1350, 1456, 1478, 1533, 1724, 1844, 2018
AseI ATTAAT 1 cut(s) 2042
AspLEI GCGC 2 cut(s) 792, 2110
AspS9I GGNCC 6 cut(s) 320, 572, 1209, 1318, 1390, 2200
AsuHPI GGTGA 3 cut(s) 725, 1724, 1733
AsuII TTCGAA 1 cut(s) 1228
AvaI CYCGRG 1 cut(s) 1249
AvaII GGWCC 5 cut(s) 320, 572, 1209, 1318, 2200
BaeI ACNNNNGTAYC 2 cut(s) 1923, 1956
BalI TGGCCA 3 cut(s) 367, 457, 2003
BanI GGYRCC 1 cut(s) 1037
BanII GRGCYC 2 cut(s) 295, 861
BbsI GAAGAC 3 cut(s) 357, 409, 617
Bbv12I GWGCWC 4 cut(s) 514, 730, 861, 2098
BbvI GCAGC 7 cut(s) 190, 246, 391, 604, 1783, 2020, 2065
BccI CCATC 2 cut(s) 1227, 2152
BceAI ACGGC 2 cut(s) 511, 786
BciT130I CCWGG 5 cut(s) 395, 480, 570, 1157, 1388
BciVI GTATCC 1 cut(s) 911
BclI TGATCA 2 cut(s) 250, 1525
BcoDI GTCTC 7 cut(s) 101, 389, 542, 1252, 1429, 1881, 2019
BcuI ACTAGT 1 cut(s) 715
BfaI CTAG 2 cut(s) 716, 1883
BfmI CTRYAG 2 cut(s) 973, 1791
BfuAI ACCTGC 1 cut(s) 1315
BfuI GTATCC 1 cut(s) 911
BglII AGATCT 2 cut(s) 54, 766
BisI GCNGC 8 cut(s) 27, 204, 235, 405, 618, 1772, 2009, 2079
BlsI GCNGC 8 cut(s) 28, 205, 236, 406, 619, 1773, 2010, 2080
BmcAI AGTACT 2 cut(s) 602, 2193
Bme1390I CCNGG 5 cut(s) 395, 480, 570, 1157, 1388
Bme18I GGWCC 5 cut(s) 320, 572, 1209, 1318, 2200
BmeT110I CYCGRG 1 cut(s) 1249
BmgT120I GGNCC 6 cut(s) 320, 572, 1209, 1318, 1390, 2200
BmiI GGNNCC 4 cut(s) 259, 294, 567, 1039
BmrFI CCNGG 5 cut(s) 395, 480, 570, 1157, 1388
BmrI ACTGGG 1 cut(s) 208
BmsI GCATC 6 cut(s) 823, 902, 1042, 1510, 1603, 1978
BmuI ACTGGG 1 cut(s) 208
BpiI GAAGAC 3 cut(s) 357, 409, 617
BplI GAGNNNNNCTC 2 cut(s) 590, 622
BpmI CTGGAG 3 cut(s) 1178, 1223, 2216
Bpu14I TTCGAA 1 cut(s) 1228
BpuEI CTTGAG 4 cut(s) 154, 524, 592, 1379
Bsa29I ATCGAT 1 cut(s) 845
BsaBI GATNNNNATC 3 cut(s) 783, 1524, 1867
BsaI GGTCTC 1 cut(s) 389
BsaJI CCNNGG 6 cut(s) 313, 368, 750, 1387, 1488, 1558
BsaWI WCCGGW 3 cut(s) 562, 574, 1597
BsaXI ACNNNNNCTCC 2 cut(s) 2121, 2151
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 569, 2194
Bse118I RCCGGY 2 cut(s) 620, 681
Bse1I ACTGG 3 cut(s) 203, 1946, 2199
Bse3DI GCAATG 5 cut(s) 101, 414, 799, 1711, 2086
Bse8I GATNNNNATC 3 cut(s) 783, 1524, 1867
BseAI TCCGGA 1 cut(s) 1597
BseBI CCWGG 5 cut(s) 395, 480, 570, 1157, 1388
BseCI ATCGAT 1 cut(s) 845
BseDI CCNNGG 6 cut(s) 313, 368, 750, 1387, 1488, 1558
BseGI GGATG 4 cut(s) 601, 935, 1594, 2184
BseJI GATNNNNATC 3 cut(s) 783, 1524, 1867
BseLI CCNNNNNNNGG 3 cut(s) 269, 569, 2194
BseMI GCAATG 5 cut(s) 101, 414, 799, 1711, 2086
BseMII CTCAG 3 cut(s) 672, 846, 1665
BseNI ACTGG 3 cut(s) 203, 1946, 2199
BseRI GAGGAG 2 cut(s) 595, 2192
BseXI GCAGC 7 cut(s) 190, 246, 391, 604, 1783, 2020, 2065
BseYI CCCAGC 1 cut(s) 685
Bsh1236I CGCG 3 cut(s) 508, 1650, 2108
BshFI GGCC 4 cut(s) 367, 457, 1392, 2003
BshNI GGYRCC 1 cut(s) 1037
BshVI ATCGAT 1 cut(s) 845
BsiHKAI GWGCWC 4 cut(s) 514, 730, 861, 2098
BsiHKCI CYCGRG 1 cut(s) 1249
BsiSI CCGG 5 cut(s) 563, 575, 621, 682, 1598
BslI CCNNNNNNNGG 3 cut(s) 269, 569, 2194
BsmAI GTCTC 7 cut(s) 101, 389, 542, 1252, 1429, 1881, 2019
BsmBI CGTCTC 1 cut(s) 1881
BsnI GGCC 4 cut(s) 367, 457, 1392, 2003
Bso31I GGTCTC 1 cut(s) 389
BsoBI CYCGRG 1 cut(s) 1249
Bsp119I TTCGAA 1 cut(s) 1228
Bsp1286I GDGCHC 5 cut(s) 295, 514, 730, 861, 2098
Bsp13I TCCGGA 1 cut(s) 1597
Bsp1407I TGTACA 1 cut(s) 882
Bsp143I GATC 9 cut(s) 54, 250, 766, 1138, 1169, 1292, 1341, 1424, 1525
Bsp19I CCATGG 1 cut(s) 750
BspACI CCGC 5 cut(s) 27, 76, 915, 1207, 1796
BspANI GGCC 4 cut(s) 367, 457, 1392, 2003
BspCNI CTCAG 3 cut(s) 671, 847, 1666
BspDI ATCGAT 1 cut(s) 845
BspEI TCCGGA 1 cut(s) 1597
BspFNI CGCG 3 cut(s) 508, 1650, 2108
BspHI TCATGA 1 cut(s) 1143
BspLI GGNNCC 4 cut(s) 259, 294, 567, 1039
BspMI ACCTGC 1 cut(s) 1315
BspPI GGATC 2 cut(s) 1349, 1419
BspT104I TTCGAA 1 cut(s) 1228
BspT107I GGYRCC 1 cut(s) 1037
BspTNI GGTCTC 1 cut(s) 389
BsrBI CCGCTC 2 cut(s) 29, 1207
BsrDI GCAATG 5 cut(s) 101, 414, 799, 1711, 2086
BsrFI RCCGGY 2 cut(s) 620, 681
BsrGI TGTACA 1 cut(s) 882
BsrI ACTGG 3 cut(s) 203, 1946, 2199
BssAI RCCGGY 2 cut(s) 620, 681
BssECI CCNNGG 6 cut(s) 313, 368, 750, 1387, 1488, 1558
BssMI GATC 9 cut(s) 54, 250, 766, 1138, 1169, 1292, 1341, 1424, 1525
BssNAI GTATAC 1 cut(s) 1105
BssT1I CCWWGG 4 cut(s) 313, 368, 750, 1558
Bst1107I GTATAC 1 cut(s) 1105
Bst2UI CCWGG 5 cut(s) 395, 480, 570, 1157, 1388
Bst4CI ACNGT 2 cut(s) 453, 1828
BstAPI GCANNNNNTGC 1 cut(s) 1520
BstAUI TGTACA 1 cut(s) 882
BstBI TTCGAA 1 cut(s) 1228
BstC8I GCNNGC 7 cut(s) 192, 232, 500, 510, 526, 1776, 2164
BstDEI CTNAG 4 cut(s) 658, 855, 1370, 1674
BstDSI CCRYGG 1 cut(s) 750
BstF5I GGATG 4 cut(s) 601, 935, 1594, 2184
BstFNI CGCG 3 cut(s) 508, 1650, 2108
BstHHI GCGC 2 cut(s) 792, 2110
BstKTI GATC 9 cut(s) 57, 253, 769, 1141, 1172, 1295, 1344, 1427, 1528
BstMAI GTCTC 7 cut(s) 101, 389, 542, 1252, 1429, 1881, 2019
BstMBI GATC 9 cut(s) 54, 250, 766, 1138, 1169, 1292, 1341, 1424, 1525
BstMWI GCNNNNNNNGC 7 cut(s) 23, 200, 212, 454, 798, 1052, 1520
BstNI CCWGG 5 cut(s) 395, 480, 570, 1157, 1388
BstNSI RCATGY 1 cut(s) 1995
BstSCI CCNGG 5 cut(s) 393, 478, 568, 1155, 1386
BstSFI CTRYAG 2 cut(s) 973, 1791
BstUI CGCG 3 cut(s) 508, 1650, 2108
BstV1I GCAGC 7 cut(s) 190, 246, 391, 604, 1783, 2020, 2065
BstV2I GAAGAC 3 cut(s) 357, 409, 617
BstX2I RGATCY 3 cut(s) 54, 766, 1424
BstYI RGATCY 3 cut(s) 54, 766, 1424
BstZ17I GTATAC 1 cut(s) 1105
Bsu15I ATCGAT 1 cut(s) 845
BsuI GTATCC 1 cut(s) 911
BsuRI GGCC 4 cut(s) 367, 457, 1392, 2003
BsuTUI ATCGAT 1 cut(s) 845
BtgI CCRYGG 1 cut(s) 750
BtgZI GCGATG 1 cut(s) 928
BtsCI GGATG 4 cut(s) 601, 935, 1594, 2184
BtsIMutI CAGTG 2 cut(s) 246, 1134
BveI ACCTGC 1 cut(s) 1315
Cac8I GCNNGC 7 cut(s) 192, 232, 500, 510, 526, 1776, 2164
CciI TCATGA 1 cut(s) 1143
CfoI GCGC 2 cut(s) 792, 2110
Cfr10I RCCGGY 2 cut(s) 620, 681
Cfr13I GGNCC 6 cut(s) 320, 572, 1209, 1318, 1390, 2200
ClaI ATCGAT 1 cut(s) 845
CseI GACGC 1 cut(s) 1656
CviAII CATG 8 cut(s) 592, 751, 989, 1144, 1173, 1718, 1922, 1992
DdeI CTNAG 4 cut(s) 658, 855, 1370, 1674
DpnI GATC 9 cut(s) 56, 252, 768, 1140, 1171, 1294, 1343, 1426, 1527
DpnII GATC 9 cut(s) 54, 250, 766, 1138, 1169, 1292, 1341, 1424, 1525
DraI TTTAAA 2 cut(s) 1483, 1852
EaeI YGGCCR 3 cut(s) 365, 455, 2001
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 4 cut(s) 313, 368, 750, 1558
Eco24I GRGCYC 2 cut(s) 295, 861
Eco31I GGTCTC 1 cut(s) 389
Eco47I GGWCC 5 cut(s) 320, 572, 1209, 1318, 2200
Eco53kI GAGCTC 1 cut(s) 859
Eco88I CYCGRG 1 cut(s) 1249
EcoICRI GAGCTC 1 cut(s) 859
EcoRII CCWGG 5 cut(s) 393, 478, 568, 1155, 1386
EcoT14I CCWWGG 4 cut(s) 313, 368, 750, 1558
EcoT22I ATGCAT 1 cut(s) 1993
EcoT38I GRGCYC 2 cut(s) 295, 861
ErhI CCWWGG 4 cut(s) 313, 368, 750, 1558
Esp3I CGTCTC 1 cut(s) 1881
FaeI CATG 8 cut(s) 595, 754, 992, 1147, 1176, 1721, 1925, 1995
FatI CATG 8 cut(s) 591, 750, 988, 1143, 1172, 1717, 1921, 1991
FauI CCCGC 1 cut(s) 1789
FauNDI CATATG 1 cut(s) 2074
FbaI TGATCA 2 cut(s) 250, 1525
FblI GTMKAC 1 cut(s) 1104
Fnu4HI GCNGC 8 cut(s) 27, 204, 235, 405, 618, 1772, 2009, 2079
FokI GGATG 4 cut(s) 608, 922, 1581, 2171
FriOI GRGCYC 2 cut(s) 295, 861
Fsp4HI GCNGC 8 cut(s) 27, 204, 235, 405, 618, 1772, 2009, 2079
FspBI CTAG 2 cut(s) 716, 1883
GlaI GCGC 2 cut(s) 791, 2109
GluI GCNGC 8 cut(s) 27, 204, 235, 405, 618, 1772, 2009, 2079
GsaI CCCAGC 1 cut(s) 689
GsuI CTGGAG 3 cut(s) 1178, 1223, 2216
HaeIII GGCC 4 cut(s) 367, 457, 1392, 2003
HapII CCGG 5 cut(s) 563, 575, 621, 682, 1598
HgaI GACGC 1 cut(s) 1656
HhaI GCGC 2 cut(s) 792, 2110
Hin1II CATG 8 cut(s) 595, 754, 992, 1147, 1176, 1721, 1925, 1995
Hin6I GCGC 2 cut(s) 790, 2108
HinP1I GCGC 2 cut(s) 790, 2108
HindIII AAGCTT 1 cut(s) 891
HinfI GANTC 9 cut(s) 70, 662, 824, 1241, 1374, 1447, 1800, 1868, 2027
HpaII CCGG 5 cut(s) 563, 575, 621, 682, 1598
HphI GGTGA 3 cut(s) 725, 1724, 1733
Hpy166II GTNNAC 2 cut(s) 885, 1105
Hpy8I GTNNAC 2 cut(s) 885, 1105
Hpy99I CGWCG 1 cut(s) 1650
HpyAV CCTTC 6 cut(s) 121, 641, 686, 949, 1209, 1332
HpyCH4III ACNGT 2 cut(s) 453, 1828
HpyCH4IV ACGT 2 cut(s) 414, 1736
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 200, 212, 454, 798, 1052, 1520
HpyF3I CTNAG 4 cut(s) 658, 855, 1370, 1674
HpySE526I ACGT 2 cut(s) 414, 1736
Hsp92II CATG 8 cut(s) 595, 754, 992, 1147, 1176, 1721, 1925, 1995
HspAI GCGC 2 cut(s) 790, 2108
Kpn2I TCCGGA 1 cut(s) 1597
Ksp22I TGATCA 2 cut(s) 250, 1525
Kzo9I GATC 9 cut(s) 54, 250, 766, 1138, 1169, 1292, 1341, 1424, 1525
LmnI GCTCC 5 cut(s) 34, 78, 298, 1204, 2054
Lsp1109I GCAGC 7 cut(s) 190, 246, 391, 604, 1783, 2020, 2065
LweI GCATC 6 cut(s) 823, 902, 1042, 1510, 1603, 1978
MaeI CTAG 2 cut(s) 716, 1883
MaeII ACGT 2 cut(s) 414, 1736
MaeIII GTNAC 6 cut(s) 673, 731, 1132, 1633, 1910, 2151
MalI GATC 9 cut(s) 56, 252, 768, 1140, 1171, 1294, 1343, 1426, 1527
MbiI CCGCTC 2 cut(s) 29, 1207
MboI GATC 9 cut(s) 54, 250, 766, 1138, 1169, 1292, 1341, 1424, 1525
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 3 cut(s) 54, 766, 1424
MhlI GDGCHC 5 cut(s) 295, 514, 730, 861, 2098
MlsI TGGCCA 3 cut(s) 367, 457, 2003
MluI ACGCGT 2 cut(s) 506, 1648
MluNI TGGCCA 3 cut(s) 367, 457, 2003
MlyI GAGTC 2 cut(s) 671, 2021
MmeI TCCRAC 2 cut(s) 991, 1327
MnlI CCTC 9 cut(s) 616, 644, 1403, 1498, 1798, 1897, 2170, 2173, 2191
Mox20I TGGCCA 3 cut(s) 367, 457, 2003
Mph1103I ATGCAT 1 cut(s) 1993
MroI TCCGGA 1 cut(s) 1597
MscI TGGCCA 3 cut(s) 367, 457, 2003
MseI TTAA 8 cut(s) 92, 116, 1164, 1482, 1763, 1851, 1941, 2042
MslI CAYNNNNRTG 2 cut(s) 315, 2238
Msp20I TGGCCA 3 cut(s) 367, 457, 2003
MspA1I CMGCKG 1 cut(s) 2011
MspI CCGG 5 cut(s) 563, 575, 621, 682, 1598
MspR9I CCNGG 5 cut(s) 395, 480, 570, 1157, 1388
MunI CAATTG 1 cut(s) 339
MvaI CCWGG 5 cut(s) 395, 480, 570, 1157, 1388
MvnI CGCG 3 cut(s) 508, 1650, 2108
MwoI GCNNNNNNNGC 7 cut(s) 23, 200, 212, 454, 798, 1052, 1520
NcoI CCATGG 1 cut(s) 750
NdeI CATATG 1 cut(s) 2074
NdeII GATC 9 cut(s) 54, 250, 766, 1138, 1169, 1292, 1341, 1424, 1525
NlaIII CATG 8 cut(s) 595, 754, 992, 1147, 1176, 1721, 1925, 1995
NlaIV GGNNCC 4 cut(s) 259, 294, 567, 1039
NmuCI GTSAC 3 cut(s) 673, 731, 1132
NsiI ATGCAT 1 cut(s) 1993
NspI RCATGY 1 cut(s) 1995
NspV TTCGAA 1 cut(s) 1228
OliI CACNNNNGTG 1 cut(s) 315
PagI TCATGA 1 cut(s) 1143
PfeI GAWTC 7 cut(s) 70, 824, 1241, 1374, 1447, 1800, 1868
PflFI GACNNNGTC 1 cut(s) 1738
PflMI CCANNNNNTGG 2 cut(s) 269, 2194
PkrI GCNGC 8 cut(s) 28, 205, 236, 406, 619, 1773, 2010, 2080
PleI GAGTC 2 cut(s) 670, 2021
PpsI GAGTC 2 cut(s) 670, 2021
PshBI ATTAAT 1 cut(s) 2042
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 5 cut(s) 393, 478, 568, 1155, 1386
PspFI CCCAGC 1 cut(s) 685
PspGI CCWGG 5 cut(s) 393, 478, 568, 1155, 1386
PspN4I GGNNCC 4 cut(s) 259, 294, 567, 1039
PspPI GGNCC 6 cut(s) 320, 572, 1209, 1318, 1390, 2200
PsuI RGATCY 3 cut(s) 54, 766, 1424
PsyI GACNNNGTC 1 cut(s) 1738
PvuII CAGCTG 1 cut(s) 2011
RseI CAYNNNNRTG 2 cut(s) 315, 2238
SacI GAGCTC 1 cut(s) 861
SaqAI TTAA 8 cut(s) 92, 116, 1164, 1482, 1763, 1851, 1941, 2042
SatI GCNGC 8 cut(s) 27, 204, 235, 405, 618, 1772, 2009, 2079
Sau3AI GATC 9 cut(s) 54, 250, 766, 1138, 1169, 1292, 1341, 1424, 1525
Sau96I GGNCC 6 cut(s) 320, 572, 1209, 1318, 1390, 2200
ScaI AGTACT 2 cut(s) 602, 2193
SchI GAGTC 2 cut(s) 671, 2021
ScrFI CCNGG 5 cut(s) 395, 480, 570, 1157, 1388
SduI GDGCHC 5 cut(s) 295, 514, 730, 861, 2098
SfaNI GCATC 6 cut(s) 823, 902, 1042, 1510, 1603, 1978
SfcI CTRYAG 2 cut(s) 973, 1791
SfuI TTCGAA 1 cut(s) 1228
SinI GGWCC 5 cut(s) 320, 572, 1209, 1318, 2200
SmiI ATTTAAAT 1 cut(s) 1483
SmiMI CAYNNNNRTG 2 cut(s) 315, 2238
SmlI CTYRAG 4 cut(s) 133, 539, 607, 1394
SmoI CTYRAG 4 cut(s) 133, 539, 607, 1394
SpeI ACTAGT 1 cut(s) 715
SsiI CCGC 5 cut(s) 27, 76, 915, 1207, 1796
SspI AATATT 1 cut(s) 709
SspMI CTAG 2 cut(s) 716, 1883
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 5 cut(s) 393, 478, 568, 1155, 1386
StyI CCWWGG 4 cut(s) 313, 368, 750, 1558
SwaI ATTTAAAT 1 cut(s) 1483
TaaI ACNGT 2 cut(s) 453, 1828
TaiI ACGT 2 cut(s) 417, 1739
TaqI TCGA 7 cut(s) 149, 777, 845, 910, 1228, 1405, 2030
TaqII GACCGA 1 cut(s) 1659
TatI WGTACW 5 cut(s) 588, 600, 882, 1602, 2191
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 7 cut(s) 70, 824, 1241, 1374, 1447, 1800, 1868
Tru1I TTAA 8 cut(s) 92, 116, 1164, 1482, 1763, 1851, 1941, 2042
Tru9I TTAA 8 cut(s) 92, 116, 1164, 1482, 1763, 1851, 1941, 2042
TscAI CASTG 2 cut(s) 253, 1141
TseFI GTSAC 3 cut(s) 673, 731, 1132
TseI GCWGC 7 cut(s) 203, 234, 404, 617, 1771, 2008, 2078
Tsp45I GTSAC 3 cut(s) 673, 731, 1132
TspGWI ACGGA 2 cut(s) 1905, 2041
TspRI CASTG 2 cut(s) 253, 1141
Tth111I GACNNNGTC 1 cut(s) 1738
Van91I CCANNNNNTGG 2 cut(s) 269, 2194
VpaK11BI GGWCC 5 cut(s) 320, 572, 1209, 1318, 2200
VspI ATTAAT 1 cut(s) 2042
XapI RAATTY 7 cut(s) 1350, 1456, 1478, 1533, 1724, 1844, 2018
XceI RCATGY 1 cut(s) 1995
XcmI CCANNNNNNNNNTGG 1 cut(s) 895
XmiI GTMKAC 1 cut(s) 1104
XspI CTAG 2 cut(s) 716, 1883
ZrmI AGTACT 2 cut(s) 602, 2193
Zsp2I ATGCAT 1 cut(s) 1993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.