Rh2DG245500

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000662
Physical Location & Seq
Reverse (-)
25107170 .. 25111418
4249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG245500.1

Sequence Viewer

Length: 2277 bp
ATGGCTTCCTCAGCGGCTCCTATTTCTGACAGCCTCAAGAAGATCTGGGATCAGTGGAATCTCCGGAGCTTCATCATGTTAAGTCTCACATTGCAGACCTTCTTAATTCTCTTTGCACCCTTGAGAAAGCGATCATCGAGCAAATGGGTAATCGTGCCCATCTGGTTGGCTTACTTGCTCGCTGACTGGGCTGCTAACTTCGCTATCGGACTAATTGCAAGCAGCCAAACGAACACTGATCAAGGTTCGCCAGAGAATGGTAATCTATTGGCATTCTGGGCTCCCTTTCTTCTTTTGCACCTTGGTGGTCCAGACACCATAATTGCAATTGCTATGGAAGACAATGCGTTGTGGCCAAGGCACTTGCTTGGTCTCATATTCCAGGTTGTAGCTGCAATGTACGTCTTCGGTATGCAGTTCTCTAACCAAAACAAGCTACTGTGGCCAACACTCTTCCTGTTTGTACCAGGACTTGTCAAGTACGGCGAGCGAACACGTGCTTTATATCTTGCCAGCTTGGAAAGTCTCAAGGAAACCATACTTGCAGTACCGGAACCCGGACCGGATTATGCAAAGTACATGGATGAGTACTCCTCAAGAAAAGCTGCCCATCTTCCAATTAAAATAGAAATTGAAGAAGGTCGTAGCTCAGATTCTCAGAATGTGACTTACCGGCTGGGAGAAGGAGTCATTGATAATATTACACTAGTGGAGGGTGCTCATCACTTGTTTCAGATTTCCATGGGTCTTATTATAGATCTTGTGTTTGACTATCATCAGCGCAACGAGAGCCGTGCCTATTTCAAAATATTGAAACCAGAAGATGCTTTTACATCAATTGCGACTGAGCTTAATTTTATGTATGAAGCTCTGTACACCAAAGTTTTTGTGCTACATTCTCGACGGGGATACTTGTTGAGAGCTACATCCTTCATCGCCGTTTGCATAGCCTTTGTTTTCTTCTACAGATTAGAGAAACATGGCTTTCAAAGGTTTGATGTTGGAATTACTTATTCCTTGCTTTTTGGTGCCATAGCTCTTGATTCAATAGCTCTTTCGATGCTGATATTATCTGATTGGACGATCGCTGTTGTATACAAGTACTGTGAAAAGTGTTGTGTAGTCACTGATCTCATGATGAAGTACCTGGAGTTTAAGAGATCATGGCGACCAACTACTCCAAAGTATCTGGAGCGGACCAGAAAGATTTTATTTCGAAGATGGTGTGAATCTGTCTCCGGTTTCAGCTTCATAGATTATTCTCTCCGAGAATGTGGAAGATCAGCAGTCCGACATGCAATTTTGGACCAAATTGGCATTTACCGAAGGATCATCAGATTTTTGGGCTACCTTTACCATAGAATCATCATATTCCTAGGCCACCTTTACCTCAGAATCATTAAATTCTTTGGCCTGAAGTATCTTCGAGATGAGATGAAGTATAGATCCAGCAAGAGACTGGAAGAGAATTTGTGGAAGTTCATTTTTGATGAGTTAAAAAGTAAATCCTCAGAAGTAGTTGATGATCCCAAAATTGGTAGAGAAATATGTTCGTCCCAAGGGGACTGGGTTCTCCTAAAAAAGTATGAGAAGCATCCGGAATACAAGAGCTTTCTATCTTGTGAGGAATTACAATCGTGCTTTGTTGATGTTACTTATGACCGACGCATTCTGCTGTGGCATATTGCTACTGAGATGTGCTATTACAGAGTTGATACAGGCAATGGAGGTGATAATCGTAAATACAGTAAGACGTTGTCTGATTATATGCTATATCTCTTAATAATGCAGCCTGCTTTGATGTCCTCTGTAGCGGGAATCGGACAAAAGAGATTTAGAGATACGGTTGCAGAAGCCAATAAATTCTTTAAAAAAAGGGGATTGGGTTCCGATGTGAAACTAAGCGAGGGGAAACTACGCGAGGCTTGTTGCAAACTCCTTGATGTAGATACAGGAGTTAAACCAGTTGATGTCAAAGGGAATAGAAGTAAATCTGTTTTGTTTGACGCATGTATTTTGGCCAGGCAGCTGATGAAATTCCAAGACAAGAAATGGGACTTAATAAGCTCGGTGTGGGTGGAATTGCTATCATATGCTGCAAGTCATTGCAGAGCACATAGCCACGCGCAATTGCTAACTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTACTGATGGCTCGCTTTGGCATTGAGGAGGACATCCAAGTACTGGAGGTCCAAGCAAGAGTAAAACTGATCATGGAAAAGCAACCTGATGTATTCCAGAGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

758

Amino Acids

87.51

Weight (kDa)

9.01

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 420 8.3e-91 Domain of unknown function (DUF4220)
DUF594 PF04578 681 - 731 4.3e-21 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1028
AccB7I CCANNNNNTGG 2 cut(s) 257, 2212
AccBSI CCGCTC 1 cut(s) 1195
AccI GTMKAC 1 cut(s) 1095
AccII CGCG 2 cut(s) 1920, 2126
AccIII TCCGGA 2 cut(s) 63, 1597
AciI CCGC 3 cut(s) 14, 1195, 1814
AclWI GGATC 4 cut(s) 57, 1337, 1440, 1520
AcoI YGGCCR 3 cut(s) 353, 443, 2019
AcsI RAATTY 4 cut(s) 1403, 1468, 1862, 2036
AcuI CTGAAG 1 cut(s) 1436
AcvI CACGTG 1 cut(s) 497
AfiI CCNNNNNNNGG 4 cut(s) 257, 557, 1535, 2212
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 5 cut(s) 635, 805, 814, 989, 1047
AhlI ACTAGT 1 cut(s) 706
AjnI CCWGG 4 cut(s) 381, 466, 1146, 2021
AleI CACNNNNGTG 1 cut(s) 303
Alw21I GWGCWC 2 cut(s) 721, 2116
Alw26I GTCTC 5 cut(s) 89, 377, 530, 1240, 1450
AlwI GGATC 4 cut(s) 57, 1337, 1440, 1520
Aor13HI TCCGGA 2 cut(s) 63, 1597
AoxI GGCC 5 cut(s) 353, 443, 1378, 1411, 2019
ApeKI GCWGC 7 cut(s) 191, 222, 392, 605, 1789, 2026, 2096
ApoI RAATTY 4 cut(s) 1403, 1468, 1862, 2036
AspA2I CCTAGG 1 cut(s) 1375
AspLEI GCGC 2 cut(s) 783, 2128
AspS9I GGNCC 5 cut(s) 308, 560, 1197, 1306, 2218
AsuC2I CCSGG 1 cut(s) 558
AsuHPI GGTGA 1 cut(s) 1742
AsuII TTCGAA 1 cut(s) 1216
AvaII GGWCC 5 cut(s) 308, 560, 1197, 1306, 2218
AvrII CCTAGG 1 cut(s) 1375
BaeGI GKGCMC 1 cut(s) 159
BaeI ACNNNNGTAYC 2 cut(s) 1941, 1974
BalI TGGCCA 3 cut(s) 355, 445, 2021
BanI GGYRCC 1 cut(s) 1028
BanII GRGCYC 1 cut(s) 283
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 2 cut(s) 345, 397
Bbv12I GWGCWC 2 cut(s) 721, 2116
BbvCI CCTCAGC 1 cut(s) 10
BbvI GCAGC 7 cut(s) 178, 234, 379, 592, 1801, 2038, 2083
BccI CCATC 4 cut(s) 167, 618, 1215, 2170
BceAI ACGGC 3 cut(s) 499, 777, 923
BcgI CGANNNNNNTGC 2 cut(s) 776, 810
BciT130I CCWGG 4 cut(s) 383, 468, 1148, 2023
BciVI GTATCC 1 cut(s) 902
BclI TGATCA 2 cut(s) 238, 2238
BcnI CCSGG 1 cut(s) 558
BcoDI GTCTC 5 cut(s) 89, 377, 530, 1240, 1450
BcuI ACTAGT 1 cut(s) 706
BfaI CTAG 2 cut(s) 707, 1376
BfmI CTRYAG 2 cut(s) 964, 1809
BfuI GTATCC 1 cut(s) 902
BglII AGATCT 2 cut(s) 42, 757
BisI GCNGC 8 cut(s) 15, 192, 223, 393, 606, 1790, 2027, 2097
BlnI CCTAGG 1 cut(s) 1375
BlsI GCNGC 8 cut(s) 16, 193, 224, 394, 607, 1791, 2028, 2098
BmcAI AGTACT 3 cut(s) 590, 1103, 2211
Bme1390I CCNGG 5 cut(s) 383, 468, 558, 1148, 2023
Bme18I GGWCC 5 cut(s) 308, 560, 1197, 1306, 2218
BmgT120I GGNCC 5 cut(s) 308, 560, 1197, 1306, 2218
BmiI GGNNCC 5 cut(s) 18, 282, 555, 1030, 1888
BmrFI CCNGG 5 cut(s) 383, 468, 558, 1148, 2023
BmrI ACTGGG 2 cut(s) 196, 1576
BmsI GCATC 3 cut(s) 814, 1050, 1603
BmuI ACTGGG 2 cut(s) 196, 1576
BpiI GAAGAC 2 cut(s) 345, 397
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 3 cut(s) 1169, 1211, 2234
Bpu10I CCTNAGC 1 cut(s) 10
Bpu14I TTCGAA 1 cut(s) 1216
BpuEI CTTGAG 4 cut(s) 20, 142, 512, 580
BpuMI CCSGG 1 cut(s) 558
BsaAI YACGTR 1 cut(s) 497
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 5 cut(s) 301, 356, 741, 1375, 1558
BsaWI WCCGGW 5 cut(s) 63, 550, 562, 1238, 1597
BsaXI ACNNNNNCTCC 4 cut(s) 672, 702, 2139, 2169
Bsc4I CCNNNNNNNGG 4 cut(s) 257, 557, 1535, 2212
Bse118I RCCGGY 1 cut(s) 672
Bse1I ACTGG 5 cut(s) 191, 1464, 1571, 1964, 2217
Bse3DI GCAATG 4 cut(s) 89, 402, 1729, 2104
BseAI TCCGGA 2 cut(s) 63, 1597
BseBI CCWGG 4 cut(s) 383, 468, 1148, 2023
BseDI CCNNGG 5 cut(s) 301, 356, 741, 1375, 1558
BseGI GGATG 4 cut(s) 589, 926, 1594, 2202
BseLI CCNNNNNNNGG 4 cut(s) 257, 557, 1535, 2212
BseMI GCAATG 4 cut(s) 89, 402, 1729, 2104
BseMII CTCAG 7 cut(s) 24, 663, 671, 837, 1405, 1524, 1683
BseNI ACTGG 5 cut(s) 191, 1464, 1571, 1964, 2217
BseRI GAGGAG 2 cut(s) 583, 2210
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 7 cut(s) 178, 234, 379, 592, 1801, 2038, 2083
BseYI CCCAGC 1 cut(s) 676
Bsh1236I CGCG 2 cut(s) 1920, 2126
Bsh1285I CGRYCG 1 cut(s) 1086
BshFI GGCC 5 cut(s) 355, 445, 1380, 1413, 2021
BshNI GGYRCC 1 cut(s) 1028
BsiEI CGRYCG 1 cut(s) 1086
BsiHKAI GWGCWC 2 cut(s) 721, 2116
BsiSI CCGG 7 cut(s) 64, 551, 558, 563, 673, 1239, 1598
BslFI GGGAC 3 cut(s) 1540, 1577, 2069
BslI CCNNNNNNNGG 4 cut(s) 257, 557, 1535, 2212
BsmAI GTCTC 5 cut(s) 89, 377, 530, 1240, 1450
BsmFI GGGAC 3 cut(s) 1540, 1577, 2069
BsmI GAATGC 2 cut(s) 272, 1668
BsnI GGCC 5 cut(s) 355, 445, 1380, 1413, 2021
Bso31I GGTCTC 1 cut(s) 377
Bsp119I TTCGAA 1 cut(s) 1216
Bsp1286I GDGCHC 4 cut(s) 159, 283, 721, 2116
Bsp13I TCCGGA 2 cut(s) 63, 1597
Bsp1407I TGTACA 1 cut(s) 873
Bsp19I CCATGG 1 cut(s) 741
BspACI CCGC 3 cut(s) 14, 1195, 1814
BspANI GGCC 5 cut(s) 355, 445, 1380, 1413, 2021
BspCNI CTCAG 7 cut(s) 23, 662, 670, 838, 1404, 1523, 1684
BspEI TCCGGA 2 cut(s) 63, 1597
BspFNI CGCG 2 cut(s) 1920, 2126
BspHI TCATGA 1 cut(s) 1134
BspLI GGNNCC 5 cut(s) 18, 282, 555, 1030, 1888
BspPI GGATC 4 cut(s) 57, 1337, 1440, 1520
BspT104I TTCGAA 1 cut(s) 1216
BspT107I GGYRCC 1 cut(s) 1028
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 1195
BsrDI GCAATG 4 cut(s) 89, 402, 1729, 2104
BsrFI RCCGGY 1 cut(s) 672
BsrGI TGTACA 1 cut(s) 873
BsrI ACTGG 5 cut(s) 191, 1464, 1571, 1964, 2217
BssAI RCCGGY 1 cut(s) 672
BssECI CCNNGG 5 cut(s) 301, 356, 741, 1375, 1558
BssNAI GTATAC 1 cut(s) 1096
BssT1I CCWWGG 5 cut(s) 301, 356, 741, 1375, 1558
Bst1107I GTATAC 1 cut(s) 1096
Bst2UI CCWGG 4 cut(s) 383, 468, 1148, 2023
Bst4CI ACNGT 4 cut(s) 441, 1106, 1748, 1846
Bst6I CTCTTC 2 cut(s) 458, 1458
BstAUI TGTACA 1 cut(s) 873
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 1 cut(s) 1216
BstC8I GCNNGC 6 cut(s) 180, 220, 488, 514, 1794, 2182
BstDEI CTNAG 8 cut(s) 10, 649, 657, 846, 1391, 1510, 1692, 1901
BstDSI CCRYGG 1 cut(s) 741
BstF5I GGATG 4 cut(s) 589, 926, 1594, 2202
BstFNI CGCG 2 cut(s) 1920, 2126
BstHHI GCGC 2 cut(s) 783, 2128
BstMAI GTCTC 5 cut(s) 89, 377, 530, 1240, 1450
BstMCI CGRYCG 1 cut(s) 1086
BstMWI GCNNNNNNNGC 6 cut(s) 11, 188, 200, 278, 442, 789
BstNI CCWGG 4 cut(s) 383, 468, 1148, 2023
BstNSI RCATGY 2 cut(s) 1298, 2013
BstSCI CCNGG 5 cut(s) 381, 466, 556, 1146, 2021
BstSFI CTRYAG 2 cut(s) 964, 1809
BstSLI GKGCMC 1 cut(s) 159
BstUI CGCG 2 cut(s) 1920, 2126
BstV1I GCAGC 7 cut(s) 178, 234, 379, 592, 1801, 2038, 2083
BstV2I GAAGAC 2 cut(s) 345, 397
BstX2I RGATCY 3 cut(s) 42, 757, 1445
BstXI CCANNNNNNTGG 1 cut(s) 166
BstYI RGATCY 3 cut(s) 42, 757, 1445
BstZ17I GTATAC 1 cut(s) 1096
BsuI GTATCC 1 cut(s) 902
BsuRI GGCC 5 cut(s) 355, 445, 1380, 1413, 2021
BtgI CCRYGG 1 cut(s) 741
BtgZI GCGATG 1 cut(s) 919
BtsCI GGATG 4 cut(s) 589, 926, 1594, 2202
BtsIMutI CAGTG 3 cut(s) 59, 234, 1125
Cac8I GCNNGC 6 cut(s) 180, 220, 488, 514, 1794, 2182
CciI TCATGA 1 cut(s) 1134
CfoI GCGC 2 cut(s) 783, 2128
Cfr10I RCCGGY 1 cut(s) 672
Cfr13I GGNCC 5 cut(s) 308, 560, 1197, 1306, 2218
CpoI CGGWCCG 1 cut(s) 560
CseI GACGC 2 cut(s) 1674, 2015
CspI CGGWCCG 1 cut(s) 560
CviAII CATG 9 cut(s) 76, 580, 742, 980, 1135, 1164, 1295, 2010, 2242
DdeI CTNAG 8 cut(s) 10, 649, 657, 846, 1391, 1510, 1692, 1901
DraI TTTAAA 1 cut(s) 1870
EaeI YGGCCR 3 cut(s) 353, 443, 2019
Eam1104I CTCTTC 2 cut(s) 458, 1458
EarI CTCTTC 2 cut(s) 458, 1458
Eco130I CCWWGG 5 cut(s) 301, 356, 741, 1375, 1558
Eco24I GRGCYC 1 cut(s) 283
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 5 cut(s) 308, 560, 1197, 1306, 2218
Eco57I CTGAAG 1 cut(s) 1436
Eco72I CACGTG 1 cut(s) 497
EcoRII CCWGG 4 cut(s) 381, 466, 1146, 2021
EcoT14I CCWWGG 5 cut(s) 301, 356, 741, 1375, 1558
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 5 cut(s) 301, 356, 741, 1375, 1558
FaeI CATG 9 cut(s) 79, 583, 745, 983, 1138, 1167, 1298, 2013, 2245
FaqI GGGAC 3 cut(s) 1540, 1577, 2069
FatI CATG 9 cut(s) 75, 579, 741, 979, 1134, 1163, 1294, 2009, 2241
FauI CCCGC 1 cut(s) 1807
FauNDI CATATG 1 cut(s) 2092
FbaI TGATCA 2 cut(s) 238, 2238
FblI GTMKAC 1 cut(s) 1095
Fnu4HI GCNGC 8 cut(s) 15, 192, 223, 393, 606, 1790, 2027, 2097
FokI GGATG 4 cut(s) 596, 913, 1581, 2189
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 8 cut(s) 15, 192, 223, 393, 606, 1790, 2027, 2097
FspBI CTAG 2 cut(s) 707, 1376
GlaI GCGC 2 cut(s) 782, 2127
GluI GCNGC 8 cut(s) 15, 192, 223, 393, 606, 1790, 2027, 2097
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 3 cut(s) 1169, 1211, 2234
HaeIII GGCC 5 cut(s) 355, 445, 1380, 1413, 2021
HapII CCGG 7 cut(s) 64, 551, 558, 563, 673, 1239, 1598
HgaI GACGC 2 cut(s) 1674, 2015
HhaI GCGC 2 cut(s) 783, 2128
Hin1II CATG 9 cut(s) 79, 583, 745, 983, 1138, 1167, 1298, 2013, 2245
Hin6I GCGC 2 cut(s) 781, 2126
HinP1I GCGC 2 cut(s) 781, 2126
HinfI GANTC 8 cut(s) 58, 653, 687, 1043, 1229, 1362, 1395, 1818
HpaII CCGG 7 cut(s) 64, 551, 558, 563, 673, 1239, 1598
HphI GGTGA 1 cut(s) 1742
Hpy166II GTNNAC 2 cut(s) 876, 1096
Hpy8I GTNNAC 2 cut(s) 876, 1096
Hpy99I CGWCG 2 cut(s) 906, 1668
HpyAV CCTTC 5 cut(s) 109, 632, 677, 940, 1320
HpyCH4III ACNGT 4 cut(s) 441, 1106, 1748, 1846
HpyCH4IV ACGT 3 cut(s) 402, 496, 1754
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 188, 200, 278, 442, 789
HpyF3I CTNAG 8 cut(s) 10, 649, 657, 846, 1391, 1510, 1692, 1901
HpySE526I ACGT 3 cut(s) 402, 496, 1754
Hsp92II CATG 9 cut(s) 79, 583, 745, 983, 1138, 1167, 1298, 2013, 2245
HspAI GCGC 2 cut(s) 781, 2126
Kpn2I TCCGGA 2 cut(s) 63, 1597
Ksp22I TGATCA 2 cut(s) 238, 2238
LmnI GCTCC 4 cut(s) 22, 66, 286, 1192
Lsp1109I GCAGC 7 cut(s) 178, 234, 379, 592, 1801, 2038, 2083
LweI GCATC 3 cut(s) 814, 1050, 1603
MaeI CTAG 2 cut(s) 707, 1376
MaeII ACGT 3 cut(s) 402, 496, 1754
MaeIII GTNAC 5 cut(s) 664, 1123, 1651, 2155, 2169
MbiI CCGCTC 1 cut(s) 1195
MfeI CAATTG 3 cut(s) 327, 837, 2129
MflI RGATCY 3 cut(s) 42, 757, 1445
MhlI GDGCHC 4 cut(s) 159, 283, 721, 2116
MlsI TGGCCA 3 cut(s) 355, 445, 2021
MluNI TGGCCA 3 cut(s) 355, 445, 2021
MlyI GAGTC 1 cut(s) 696
MmeI TCCRAC 2 cut(s) 982, 1315
Mox20I TGGCCA 3 cut(s) 355, 445, 2021
MroI TCCGGA 2 cut(s) 63, 1597
MscI TGGCCA 3 cut(s) 355, 445, 2021
MslI CAYNNNNRTG 1 cut(s) 303
Msp20I TGGCCA 3 cut(s) 355, 445, 2021
MspA1I CMGCKG 2 cut(s) 14, 2029
MspI CCGG 7 cut(s) 64, 551, 558, 563, 673, 1239, 1598
MspR9I CCNGG 5 cut(s) 383, 468, 558, 1148, 2023
MunI CAATTG 3 cut(s) 327, 837, 2129
Mva1269I GAATGC 2 cut(s) 272, 1668
MvaI CCWGG 4 cut(s) 383, 468, 1148, 2023
MvnI CGCG 2 cut(s) 1920, 2126
MwoI GCNNNNNNNGC 6 cut(s) 11, 188, 200, 278, 442, 789
NciI CCSGG 1 cut(s) 558
NcoI CCATGG 1 cut(s) 741
NdeI CATATG 1 cut(s) 2092
NlaIII CATG 9 cut(s) 79, 583, 745, 983, 1138, 1167, 1298, 2013, 2245
NlaIV GGNNCC 5 cut(s) 18, 282, 555, 1030, 1888
NmuCI GTSAC 3 cut(s) 664, 1123, 2155
NspI RCATGY 2 cut(s) 1298, 2013
NspV TTCGAA 1 cut(s) 1216
OliI CACNNNNGTG 1 cut(s) 303
PagI TCATGA 1 cut(s) 1134
PctI GAATGC 2 cut(s) 272, 1668
PfeI GAWTC 7 cut(s) 58, 653, 1043, 1229, 1362, 1395, 1818
PflFI GACNNNGTC 1 cut(s) 1756
PflMI CCANNNNNTGG 2 cut(s) 257, 2212
PkrI GCNGC 8 cut(s) 16, 193, 224, 394, 607, 1791, 2028, 2098
Ple19I CGATCG 1 cut(s) 1086
PleI GAGTC 1 cut(s) 695
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 1 cut(s) 695
Ppu21I YACGTR 1 cut(s) 497
Psp6I CCWGG 4 cut(s) 381, 466, 1146, 2021
PspCI CACGTG 1 cut(s) 497
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 4 cut(s) 381, 466, 1146, 2021
PspN4I GGNNCC 5 cut(s) 18, 282, 555, 1030, 1888
PspPI GGNCC 5 cut(s) 308, 560, 1197, 1306, 2218
PsuI RGATCY 3 cut(s) 42, 757, 1445
PsyI GACNNNGTC 1 cut(s) 1756
PvuI CGATCG 1 cut(s) 1086
PvuII CAGCTG 1 cut(s) 2029
RseI CAYNNNNRTG 1 cut(s) 303
Rsr2I CGGWCCG 1 cut(s) 560
RsrII CGGWCCG 1 cut(s) 560
SatI GCNGC 8 cut(s) 15, 192, 223, 393, 606, 1790, 2027, 2097
Sau96I GGNCC 5 cut(s) 308, 560, 1197, 1306, 2218
ScaI AGTACT 3 cut(s) 590, 1103, 2211
SchI GAGTC 1 cut(s) 696
ScrFI CCNGG 5 cut(s) 383, 468, 558, 1148, 2023
SduI GDGCHC 4 cut(s) 159, 283, 721, 2116
SfaNI GCATC 3 cut(s) 814, 1050, 1603
SfcI CTRYAG 2 cut(s) 964, 1809
SfuI TTCGAA 1 cut(s) 1216
SinI GGWCC 5 cut(s) 308, 560, 1197, 1306, 2218
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 4 cut(s) 35, 121, 527, 595
SmoI CTYRAG 4 cut(s) 35, 121, 527, 595
SpeI ACTAGT 1 cut(s) 706
SsiI CCGC 3 cut(s) 14, 1195, 1814
SspI AATATT 2 cut(s) 700, 810
SspMI CTAG 2 cut(s) 707, 1376
StyD4I CCNGG 5 cut(s) 381, 466, 556, 1146, 2021
StyI CCWWGG 5 cut(s) 301, 356, 741, 1375, 1558
TaaI ACNGT 4 cut(s) 441, 1106, 1748, 1846
TaiI ACGT 3 cut(s) 405, 499, 1757
TaqI TCGA 5 cut(s) 137, 901, 1058, 1216, 1426
TaqII GACCGA 1 cut(s) 1677
TatI WGTACW 5 cut(s) 576, 588, 873, 1101, 2209
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 7 cut(s) 58, 653, 1043, 1229, 1362, 1395, 1818
TscAI CASTG 3 cut(s) 59, 241, 1132
TseFI GTSAC 3 cut(s) 664, 1123, 2155
TseI GCWGC 7 cut(s) 191, 222, 392, 605, 1789, 2026, 2096
Tsp45I GTSAC 3 cut(s) 664, 1123, 2155
TspDTI ATGAA 8 cut(s) 61, 879, 922, 1154, 1240, 1451, 1471, 2048
TspRI CASTG 3 cut(s) 59, 241, 1132
Tth111I GACNNNGTC 1 cut(s) 1756
Van91I CCANNNNNTGG 2 cut(s) 257, 2212
VpaK11BI GGWCC 5 cut(s) 308, 560, 1197, 1306, 2218
XapI RAATTY 4 cut(s) 1403, 1468, 1862, 2036
XceI RCATGY 2 cut(s) 1298, 2013
XcmI CCANNNNNNNNNTGG 1 cut(s) 1456
XmaJI CCTAGG 1 cut(s) 1375
XmiI GTMKAC 1 cut(s) 1095
XspI CTAG 2 cut(s) 707, 1376
ZrmI AGTACT 3 cut(s) 590, 1103, 2211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.