Rw2G017560

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000662
Physical Location & Seq
Reverse (-)
21677068 .. 21679490
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017560.1

Sequence Viewer

Length: 2253 bp
ATGGTTTCCTCCGCCGTTCCTTTTTCTGACACCGTGAAGAAAATATGGGATAAGTGGAACCTCCGGGGCTTCATCATGTTAAGCCTCTTATTGCAGGTCTTTTTAATTTTTTGTGCGCCCCTGAGAAAGAGAACATCAAGCAAATGGGTGATCGTGCCCATTTGGTTAGCTTACTTGCTCGCAGACTGGGCTGCTAACTTTGCCATTGGATTAATTGCAAGCAGCCAAACTGACACCAACGGTCGCCGAGACAATGGTGGCCTTTTGGCATTTTGGGCTCCATTTCTTTTGATACACCTGGGTGGTCCGGACACCATAATTGCGATTTCTATGGAAGATAACGCCTTGTGGCCAAGGCACTTTCTTGGTCTCATTTTCCAGGTTTTGGCTGTGGCGTACGTCTTCATCATGCAGTCAACTCGGGAAAACAAGCTGTGGTGGCCAACACTGTTGCTAATTTTCCCAGGACTTGTCAAGTATGGCGAGCGAACACGTGCTTTATATCTTGCCAGCTTAAGCAGTCTTAAGGACTCCATACTTGCAGTACCGGACACTGGACCCGATTATGCAAAATACATGGATGAGTACTCCTCAAGAAAAGCTGCCAATCTTCCAATTCATATACACACAGACAAAGGCCGTGGGTCAGCTGAATCCAAGATTGAGACTCTCCCTGTGGAAGAAGGCCGCGACATTAACAATATTGCTGTGGTGCAGAAGGCTTATCAATTCTTTCAAATCTCCATGGGTCTTATTATAGATCTTCTTTTTTACTTTCATCAGCGGAACGAGAGCCGTGTCTATTTCCTTGGGAGAACCCCAGAAGAGGCTTATACATTAATCGCGACTGAGCTCAACTTTATGTATGAAGCTCTTTACACTAAAGCTCATATGGTTCATTCTGTGTGGGGATATGTTTTAAGGGCTACATCATTTGGAACGGTCTGGATAGCCCTTGGTTTCTTCTATAAGTTGGACAAAAATGGCTTCCACAGGTTTGATGTTAGAGTAACCTATTCCTTGCTCTTGGGTGCCATAGCCTTGGATTCAATAGCTTTTTCAATGCTTATCCTATCTGATTGGACTGTCGTAAAGTGTGGCCAGAAGTGTTGTTCTTTAGCCACTAATCTCATGGACAAACACCTCAACTTGAAGAGAGCTTGGTGGCCTACTACTGAAAAAACAACAAAGTGGAAGATCTTATTTGGAAGATGGTCTGAATCTGTCTCCACGTTCAGCTTCATAAATTATTCTCTCCAAGAACGCAGAAGAGTGCCACCTACAGTTATGGACTATGTAGGCCTTTTGGGCACTAAAATAATCAAACACTCGTGGCTCAACAATATTTGGGTTGAGATGAAATATATACTTAGATATAGAAACCGGGAGAAACTCTCTGGGGAACTGTGGAAGTTCATTTTTGAAGAGTTGAAAAGTAAATCGGAAGATGCAGTTGATCCTCAAAATGGCAGCAAAATATGTTCAGCAAGAGGGGAGTGGATTTTTAAGGATAGGCCAGAGTTTGACATATTAAGATCGCGACTTTATCTTGGTGATGATGTTACTTATGACCGAAGCATTTTGCTGTGGCACATTGCAACCGAGTTGTGCTATTATTACGAGAAGACTCAAGAGACCAATGCTAGTGATGATAAGAAGAGCAAGGCTGGTGATGATGATGGTGCTCATCGAGAGTTGAGTAAGACATTGTCTGATTATATGTTATATCTCTTAGTTATGCAGCCTGATTTGATGTCTTCTGTAGCGGGAATTGGTCAGAAAAGATTTTCTGATACTTTTGCAGAAGCCAAACAATTCTTTGAAAAAAGGGCGCTGAGATCGGATGCATCACAAAAAGATGCTTGTAAAAAACTCCTCGAAGTAAATACAGAAATTAAACCCGTTGATGTCAAAGGGAATAGAAGCAAGTCTGTATTGTTTGATGCATGTATTCTAGCAAATAAGGAGCTGAAGAATATGGAATGGAAATTGATAAGTAAAGTGTGGGTGGAATTACTATCATATGCTGCAAGTCATTGCAGACCAAGTAGCCACGCCCAATTGCTGAGTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTATTAATGGCTCATTTTGGCATAGCAGAGCAGTTCCAAGTGCAAGAGGACCAAGCAAGAGTAAAACTGATAGTTGAAAAGCCAGCGGTTGAAGTCTTTTATTGTTCTGGTGAAAAGCCACCCGAGTCAAAAGCCACCGGGTCAATTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

750

Amino Acids

85.98

Weight (kDa)

8.87

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 417 1.8e-87 Domain of unknown function (DUF4220)
DUF594 PF04578 658 - 708 3.5e-23 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 85
AccB1I GGYRCC 1 cut(s) 1031
AccB7I CCANNNNNTGG 1 cut(s) 385
AccII CGCG 3 cut(s) 690, 845, 1540
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 5 cut(s) 12, 688, 784, 1766, 2186
AclWI GGATC 1 cut(s) 1451
AcoI YGGCCR 3 cut(s) 350, 440, 1099
AcuI CTGAAG 1 cut(s) 1991
AcvI CACGTG 1 cut(s) 494
AfaI GTAC 3 cut(s) 398, 546, 587
AfiI CCNNNNNNNGG 4 cut(s) 385, 554, 826, 1466
AflII CTTAAG 2 cut(s) 514, 524
AflIII ACRYGT 1 cut(s) 491
AjnI CCWGG 3 cut(s) 297, 378, 463
AleI CACNNNNGTG 1 cut(s) 300
Alw21I GWGCWC 2 cut(s) 855, 1687
Alw26I GTCTC 5 cut(s) 243, 374, 659, 1231, 1628
AlwI GGATC 1 cut(s) 1451
Ama87I CYCGRG 2 cut(s) 420, 2222
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 9 cut(s) 259, 350, 440, 637, 685, 1099, 1166, 1300, 1514
ApeKI GCWGC 6 cut(s) 191, 222, 602, 1470, 1741, 2027
AseI ATTAAT 3 cut(s) 212, 839, 2105
AspLEI GCGC 2 cut(s) 118, 1834
AspS9I GGNCC 3 cut(s) 305, 557, 2149
AsuC2I CCSGG 3 cut(s) 65, 1385, 2239
AsuHPI GGTGA 4 cut(s) 160, 1565, 1682, 2222
AvaI CYCGRG 2 cut(s) 420, 2222
AvaII GGWCC 3 cut(s) 305, 557, 2149
BaeGI GKGCMC 2 cut(s) 159, 1313
BalI TGGCCA 3 cut(s) 352, 442, 1101
BanI GGYRCC 1 cut(s) 1031
BanII GRGCYC 2 cut(s) 280, 855
BauI CACGAG 1 cut(s) 1330
BbrPI CACGTG 1 cut(s) 494
BbsI GAAGAC 3 cut(s) 394, 1631, 1749
Bbv12I GWGCWC 2 cut(s) 855, 1687
BbvI GCAGC 6 cut(s) 178, 234, 589, 1482, 1753, 2014
BccI CCATC 2 cut(s) 1206, 1673
BceAI ACGGC 2 cut(s) 624, 780
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 3 cut(s) 299, 380, 465
BcnI CCSGG 3 cut(s) 65, 1385, 2239
BcoDI GTCTC 5 cut(s) 243, 374, 659, 1231, 1628
BfaI CTAG 2 cut(s) 1644, 1955
BfmI CTRYAG 2 cut(s) 1281, 1761
BfoI RGCGCY 1 cut(s) 1835
BfrI CTTAAG 2 cut(s) 514, 524
BfuAI ACCTGC 1 cut(s) 85
BglI GCCNNNNNGGC 1 cut(s) 1308
BglII AGATCT 2 cut(s) 760, 1197
BisI GCNGC 7 cut(s) 192, 223, 603, 688, 1471, 1742, 2028
BlsI GCNGC 7 cut(s) 193, 224, 604, 689, 1472, 1743, 2029
BmcAI AGTACT 1 cut(s) 587
Bme1390I CCNGG 6 cut(s) 65, 299, 380, 465, 1385, 2239
Bme18I GGWCC 3 cut(s) 305, 557, 2149
BmeT110I CYCGRG 2 cut(s) 420, 2222
BmgT120I GGNCC 3 cut(s) 305, 557, 2149
BmiI GGNNCC 4 cut(s) 59, 279, 559, 1033
BmrFI CCNGG 6 cut(s) 65, 299, 380, 465, 1385, 2239
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 5 cut(s) 1438, 1834, 1849, 1856, 1933
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 3 cut(s) 394, 1631, 1749
BplI GAGNNNNNCTC 4 cut(s) 575, 607, 1379, 1411
BpuEI CTTGAG 2 cut(s) 577, 1614
BpuMI CCSGG 3 cut(s) 65, 1385, 2239
BsaAI YACGTR 1 cut(s) 494
BsaI GGTCTC 2 cut(s) 374, 1628
BsaJI CCNNGG 9 cut(s) 64, 298, 353, 463, 640, 744, 808, 955, 1041
BsaWI WCCGGW 2 cut(s) 307, 547
BsaXI ACNNNNNCTCC 2 cut(s) 2070, 2100
Bsc4I CCNNNNNNNGG 4 cut(s) 385, 554, 826, 1466
Bse1I ACTGG 2 cut(s) 191, 559
Bse3DI GCAATG 2 cut(s) 1593, 2035
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 3 cut(s) 299, 380, 465
BseDI CCNNGG 9 cut(s) 64, 298, 353, 463, 640, 744, 808, 955, 1041
BseGI GGATG 2 cut(s) 586, 1849
BseLI CCNNNNNNNGG 4 cut(s) 385, 554, 826, 1466
BseMI GCAATG 2 cut(s) 1593, 2035
BseMII CTCAG 4 cut(s) 113, 840, 1826, 2057
BseNI ACTGG 2 cut(s) 191, 559
BseRI GAGGAG 2 cut(s) 580, 1865
BseSI GKGCMC 2 cut(s) 159, 1313
BseXI GCAGC 6 cut(s) 178, 234, 589, 1482, 1753, 2014
BsgI GTGCAG 1 cut(s) 734
Bsh1236I CGCG 3 cut(s) 690, 845, 1540
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 9 cut(s) 261, 352, 442, 639, 687, 1101, 1168, 1302, 1516
BshNI GGYRCC 1 cut(s) 1031
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 855, 1687
BsiHKCI CYCGRG 2 cut(s) 420, 2222
BsiSI CCGG 5 cut(s) 64, 308, 548, 1384, 2238
BsiWI CGTACG 1 cut(s) 396
BslI CCNNNNNNNGG 4 cut(s) 385, 554, 826, 1466
BsmAI GTCTC 5 cut(s) 243, 374, 659, 1231, 1628
BsnI GGCC 9 cut(s) 261, 352, 442, 639, 687, 1101, 1168, 1302, 1516
Bso31I GGTCTC 2 cut(s) 374, 1628
BsoBI CYCGRG 2 cut(s) 420, 2222
Bsp1286I GDGCHC 5 cut(s) 159, 280, 855, 1313, 1687
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 6 cut(s) 150, 760, 1197, 1456, 1535, 1838
Bsp19I CCATGG 1 cut(s) 744
Bsp68I TCGCGA 2 cut(s) 845, 1540
BspACI CCGC 5 cut(s) 12, 688, 784, 1766, 2186
BspANI GGCC 9 cut(s) 261, 352, 442, 639, 687, 1101, 1168, 1302, 1516
BspCNI CTCAG 4 cut(s) 114, 841, 1827, 2058
BspEI TCCGGA 1 cut(s) 307
BspFNI CGCG 3 cut(s) 690, 845, 1540
BspLI GGNNCC 4 cut(s) 59, 279, 559, 1033
BspMI ACCTGC 1 cut(s) 85
BspPI GGATC 1 cut(s) 1451
BspQI GCTCTTC 1 cut(s) 1652
BspT107I GGYRCC 1 cut(s) 1031
BspTI CTTAAG 2 cut(s) 514, 524
BspTNI GGTCTC 2 cut(s) 374, 1628
BsrDI GCAATG 2 cut(s) 1593, 2035
BsrI ACTGG 2 cut(s) 191, 559
BssECI CCNNGG 9 cut(s) 64, 298, 353, 463, 640, 744, 808, 955, 1041
BssMI GATC 6 cut(s) 150, 760, 1197, 1456, 1535, 1838
BssSI CACGAG 1 cut(s) 1330
BssT1I CCWWGG 5 cut(s) 353, 744, 808, 955, 1041
Bst2BI CACGAG 1 cut(s) 1330
Bst2UI CCWGG 3 cut(s) 299, 380, 465
Bst4CI ACNGT 7 cut(s) 34, 242, 450, 943, 1087, 1285, 1407
Bst6I CTCTTC 5 cut(s) 819, 1148, 1264, 1419, 1652
BstAFI CTTAAG 2 cut(s) 514, 524
BstBAI YACGTR 1 cut(s) 494
BstC8I GCNNGC 5 cut(s) 180, 220, 485, 511, 2184
BstDEI CTNAG 6 cut(s) 122, 849, 1370, 1732, 1835, 2066
BstDSI CCRYGG 2 cut(s) 640, 744
BstF5I GGATG 2 cut(s) 586, 1849
BstFNI CGCG 3 cut(s) 690, 845, 1540
BstH2I RGCGCY 1 cut(s) 1835
BstHHI GCGC 2 cut(s) 118, 1834
BstKTI GATC 6 cut(s) 153, 763, 1200, 1459, 1538, 1841
BstMAI GTCTC 5 cut(s) 243, 374, 659, 1231, 1628
BstMBI GATC 6 cut(s) 150, 760, 1197, 1456, 1535, 1838
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 5 cut(s) 188, 200, 275, 439, 1308
BstNI CCWGG 3 cut(s) 299, 380, 465
BstNSI RCATGY 1 cut(s) 1950
BstSCI CCNGG 6 cut(s) 63, 297, 378, 463, 1383, 2237
BstSFI CTRYAG 2 cut(s) 1281, 1761
BstSLI GKGCMC 2 cut(s) 159, 1313
BstUI CGCG 3 cut(s) 690, 845, 1540
BstV1I GCAGC 6 cut(s) 178, 234, 589, 1482, 1753, 2014
BstV2I GAAGAC 3 cut(s) 394, 1631, 1749
BstX2I RGATCY 2 cut(s) 760, 1197
BstXI CCANNNNNNTGG 1 cut(s) 1042
BstYI RGATCY 2 cut(s) 760, 1197
BsuRI GGCC 9 cut(s) 261, 352, 442, 639, 687, 1101, 1168, 1302, 1516
BtgI CCRYGG 2 cut(s) 640, 744
BtsCI GGATG 2 cut(s) 586, 1849
BtsIMutI CAGTG 2 cut(s) 446, 552
BtuMI TCGCGA 2 cut(s) 845, 1540
BveI ACCTGC 1 cut(s) 85
Cac8I GCNNGC 5 cut(s) 180, 220, 485, 511, 2184
CfoI GCGC 2 cut(s) 118, 1834
Cfr13I GGNCC 3 cut(s) 305, 557, 2149
Csp6I GTAC 3 cut(s) 397, 545, 586
CspCI CAANNNNNGTGG 2 cut(s) 622, 657
CviAII CATG 6 cut(s) 76, 409, 577, 745, 1132, 1947
CviQI GTAC 3 cut(s) 397, 545, 586
DdeI CTNAG 6 cut(s) 122, 849, 1370, 1732, 1835, 2066
DpnI GATC 6 cut(s) 152, 762, 1199, 1458, 1537, 1840
DpnII GATC 6 cut(s) 150, 760, 1197, 1456, 1535, 1838
EaeI YGGCCR 3 cut(s) 350, 440, 1099
Eam1104I CTCTTC 5 cut(s) 819, 1148, 1264, 1419, 1652
EarI CTCTTC 5 cut(s) 819, 1148, 1264, 1419, 1652
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 5 cut(s) 353, 744, 808, 955, 1041
Eco147I AGGCCT 1 cut(s) 1302
Eco24I GRGCYC 2 cut(s) 280, 855
Eco31I GGTCTC 2 cut(s) 374, 1628
Eco47I GGWCC 3 cut(s) 305, 557, 2149
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 1 cut(s) 1991
Eco72I CACGTG 1 cut(s) 494
Eco88I CYCGRG 2 cut(s) 420, 2222
EcoICRI GAGCTC 1 cut(s) 853
EcoRII CCWGG 3 cut(s) 297, 378, 463
EcoT14I CCWWGG 5 cut(s) 353, 744, 808, 955, 1041
EcoT22I ATGCAT 2 cut(s) 1849, 1948
EcoT38I GRGCYC 2 cut(s) 280, 855
ErhI CCWWGG 5 cut(s) 353, 744, 808, 955, 1041
FaeI CATG 6 cut(s) 79, 412, 580, 748, 1135, 1950
FatI CATG 6 cut(s) 75, 408, 576, 744, 1131, 1946
FauI CCCGC 1 cut(s) 1759
FauNDI CATATG 2 cut(s) 891, 2023
Fnu4HI GCNGC 7 cut(s) 192, 223, 603, 688, 1471, 1742, 2028
FokI GGATG 2 cut(s) 593, 1856
FriOI GRGCYC 2 cut(s) 280, 855
Fsp4HI GCNGC 7 cut(s) 192, 223, 603, 688, 1471, 1742, 2028
FspBI CTAG 2 cut(s) 1644, 1955
GlaI GCGC 2 cut(s) 117, 1833
GluI GCNGC 7 cut(s) 192, 223, 603, 688, 1471, 1742, 2028
HaeII RGCGCY 1 cut(s) 1835
HaeIII GGCC 9 cut(s) 261, 352, 442, 639, 687, 1101, 1168, 1302, 1516
HapII CCGG 5 cut(s) 64, 308, 548, 1384, 2238
HhaI GCGC 2 cut(s) 118, 1834
Hin1II CATG 6 cut(s) 79, 412, 580, 748, 1135, 1950
Hin6I GCGC 2 cut(s) 116, 1832
HinP1I GCGC 2 cut(s) 116, 1832
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HinfI GANTC 7 cut(s) 530, 653, 667, 1046, 1220, 1627, 2225
HpaII CCGG 5 cut(s) 64, 308, 548, 1384, 2238
HphI GGTGA 4 cut(s) 160, 1565, 1682, 2222
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 8 cut(s) 28, 1078, 1219, 1444, 1714, 1779, 1792, 1843
Hpy188III TCNNGA 8 cut(s) 308, 422, 594, 844, 946, 1539, 1631, 1691
Hpy8I GTNNAC 1 cut(s) 417
HpyAV CCTTC 2 cut(s) 677, 712
HpyCH4III ACNGT 7 cut(s) 34, 242, 450, 943, 1087, 1285, 1407
HpyCH4IV ACGT 3 cut(s) 399, 493, 1232
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 200, 275, 439, 1308
HpyF3I CTNAG 6 cut(s) 122, 849, 1370, 1732, 1835, 2066
HpySE526I ACGT 3 cut(s) 399, 493, 1232
Hsp92II CATG 6 cut(s) 79, 412, 580, 748, 1135, 1950
HspAI GCGC 2 cut(s) 116, 1832
Kpn2I TCCGGA 1 cut(s) 307
Kzo9I GATC 6 cut(s) 150, 760, 1197, 1456, 1535, 1838
LguI GCTCTTC 1 cut(s) 1652
LmnI GCTCC 2 cut(s) 283, 1966
Lsp1109I GCAGC 6 cut(s) 178, 234, 589, 1482, 1753, 2014
LweI GCATC 5 cut(s) 1438, 1834, 1849, 1856, 1933
MaeI CTAG 2 cut(s) 1644, 1955
MaeII ACGT 3 cut(s) 399, 493, 1232
MaeIII GTNAC 3 cut(s) 1009, 1561, 2086
MalI GATC 6 cut(s) 152, 762, 1199, 1458, 1537, 1840
MboI GATC 6 cut(s) 150, 760, 1197, 1456, 1535, 1838
MfeI CAATTG 1 cut(s) 2060
MflI RGATCY 2 cut(s) 760, 1197
MhlI GDGCHC 5 cut(s) 159, 280, 855, 1313, 1687
MlsI TGGCCA 3 cut(s) 352, 442, 1101
MluNI TGGCCA 3 cut(s) 352, 442, 1101
MlyI GAGTC 4 cut(s) 524, 661, 1621, 2234
MmeI TCCRAC 1 cut(s) 954
Mox20I TGGCCA 3 cut(s) 352, 442, 1101
Mph1103I ATGCAT 2 cut(s) 1849, 1948
MroI TCCGGA 1 cut(s) 307
MscI TGGCCA 3 cut(s) 352, 442, 1101
MslI CAYNNNNRTG 1 cut(s) 300
Msp20I TGGCCA 3 cut(s) 352, 442, 1101
MspA1I CMGCKG 3 cut(s) 650, 784, 2186
MspCI CTTAAG 2 cut(s) 514, 524
MspI CCGG 5 cut(s) 64, 308, 548, 1384, 2238
MspR9I CCNGG 6 cut(s) 65, 299, 380, 465, 1385, 2239
MunI CAATTG 1 cut(s) 2060
MvaI CCWGG 3 cut(s) 299, 380, 465
MvnI CGCG 3 cut(s) 690, 845, 1540
MwoI GCNNNNNNNGC 5 cut(s) 188, 200, 275, 439, 1308
NciI CCSGG 3 cut(s) 65, 1385, 2239
NcoI CCATGG 1 cut(s) 744
NdeI CATATG 2 cut(s) 891, 2023
NdeII GATC 6 cut(s) 150, 760, 1197, 1456, 1535, 1838
NlaIII CATG 6 cut(s) 79, 412, 580, 748, 1135, 1950
NlaIV GGNNCC 4 cut(s) 59, 279, 559, 1033
NmeAIII GCCGAG 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 2086
NruI TCGCGA 2 cut(s) 845, 1540
NsiI ATGCAT 2 cut(s) 1849, 1948
NspI RCATGY 1 cut(s) 1950
OliI CACNNNNGTG 1 cut(s) 300
PceI AGGCCT 1 cut(s) 1302
PciSI GCTCTTC 1 cut(s) 1652
PfeI GAWTC 3 cut(s) 653, 1046, 1220
Pfl23II CGTACG 1 cut(s) 396
PflFI GACNNNGTC 1 cut(s) 1708
PflMI CCANNNNNTGG 1 cut(s) 385
PkrI GCNGC 7 cut(s) 193, 224, 604, 689, 1472, 1743, 2029
PleI GAGTC 4 cut(s) 524, 661, 1621, 2233
PmaCI CACGTG 1 cut(s) 494
PmlI CACGTG 1 cut(s) 494
PpsI GAGTC 4 cut(s) 524, 661, 1621, 2233
Ppu21I YACGTR 1 cut(s) 494
PshBI ATTAAT 3 cut(s) 212, 839, 2105
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 3 cut(s) 297, 378, 463
PspCI CACGTG 1 cut(s) 494
PspGI CCWGG 3 cut(s) 297, 378, 463
PspLI CGTACG 1 cut(s) 396
PspN4I GGNNCC 4 cut(s) 59, 279, 559, 1033
PspPI GGNCC 3 cut(s) 305, 557, 2149
PsuI RGATCY 2 cut(s) 760, 1197
PsyI GACNNNGTC 1 cut(s) 1708
PvuII CAGCTG 1 cut(s) 650
RruI TCGCGA 2 cut(s) 845, 1540
RsaI GTAC 3 cut(s) 398, 546, 587
RsaNI GTAC 3 cut(s) 397, 545, 586
RseI CAYNNNNRTG 1 cut(s) 300
SacI GAGCTC 1 cut(s) 855
SapI GCTCTTC 1 cut(s) 1652
SatI GCNGC 7 cut(s) 192, 223, 603, 688, 1471, 1742, 2028
Sau3AI GATC 6 cut(s) 150, 760, 1197, 1456, 1535, 1838
Sau96I GGNCC 3 cut(s) 305, 557, 2149
ScaI AGTACT 1 cut(s) 587
SchI GAGTC 4 cut(s) 524, 661, 1621, 2234
ScrFI CCNGG 6 cut(s) 65, 299, 380, 465, 1385, 2239
SduI GDGCHC 5 cut(s) 159, 280, 855, 1313, 1687
SfaNI GCATC 5 cut(s) 1438, 1834, 1849, 1856, 1933
SfcI CTRYAG 2 cut(s) 1281, 1761
SinI GGWCC 3 cut(s) 305, 557, 2149
SmiMI CAYNNNNRTG 1 cut(s) 300
SmlI CTYRAG 4 cut(s) 514, 524, 592, 1629
SmoI CTYRAG 4 cut(s) 514, 524, 592, 1629
SseBI AGGCCT 1 cut(s) 1302
SsiI CCGC 5 cut(s) 12, 688, 784, 1766, 2186
SspI AATATT 2 cut(s) 703, 1345
SspMI CTAG 2 cut(s) 1644, 1955
SstI GAGCTC 1 cut(s) 855
StuI AGGCCT 1 cut(s) 1302
StyD4I CCNGG 6 cut(s) 63, 297, 378, 463, 1383, 2237
StyI CCWWGG 5 cut(s) 353, 744, 808, 955, 1041
TaaI ACNGT 7 cut(s) 34, 242, 450, 943, 1087, 1285, 1407
TaiI ACGT 3 cut(s) 402, 496, 1235
TaqI TCGA 2 cut(s) 1690, 1878
TaqII GACCGA 1 cut(s) 1587
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 690
TfiI GAWTC 3 cut(s) 653, 1046, 1220
TscAI CASTG 2 cut(s) 453, 559
TseFI GTSAC 1 cut(s) 2086
TseI GCWGC 6 cut(s) 191, 222, 602, 1470, 1741, 2027
Tsp45I GTSAC 1 cut(s) 2086
TspDTI ATGAA 9 cut(s) 61, 394, 608, 767, 882, 887, 1231, 1373, 1405
TspRI CASTG 2 cut(s) 453, 559
Tth111I GACNNNGTC 1 cut(s) 1708
Van91I CCANNNNNTGG 1 cut(s) 385
Vha464I CTTAAG 2 cut(s) 514, 524
VpaK11BI GGWCC 3 cut(s) 305, 557, 2149
VspI ATTAAT 3 cut(s) 212, 839, 2105
XceI RCATGY 1 cut(s) 1950
XcmI CCANNNNNNNNNTGG 1 cut(s) 1129
XspI CTAG 2 cut(s) 1644, 1955
ZrmI AGTACT 1 cut(s) 587
Zsp2I ATGCAT 2 cut(s) 1849, 1948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.