Rh2AG227300

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000662
Physical Location & Seq
Reverse (-)
24067357 .. 24078567
11211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG227300.1

Sequence Viewer

Length: 2403 bp
ATGGTTTCCTCCGCCATTCCTATTTCTGACACCGTGAAGAAAATATGGGATAAGTGGAACCTCCGGGGCTTCGTCTTATTAAGCCTCTCATTGCAGGCCTTTTTAATTCTTTGTGCACCCATGAGAAAGAGAACATCAAGCAAATGGGTGATCGTGCCCATTTGGTTAGCTTACTTGCTCGCAGACTGGGCTGCTAACTTTGCCATTGGATTAATTGCAAGCAGCCAAACTGACACCAACGGTCGCCGAGACAATGGCGGCCTTTTGGCATTTTGGGCTCCATTTCTTTTGTTACACCTGGGTGGTCCGGACACCATAATTGCGATTTCTATGGAAGATAACGCCTTGTGGCCAAGACACTTTCTTGGTCTCATATTCCAGGTTTTGGCTGTGACGTACGTCTTCATTATGCAGTCAACTCGAGAAAACAAGCTATGGTGGCCAACACTCTTGCTAATTTTCCCAGGACTTGTCAAGTATGGCGAACGAACACGTGCTTTATATCTTGCCAGCTTAAGCAGTCTCAAGGACTCCATACTTGCAGTACCGGACACTGGACCGGATTATGCAAAGTACATGGATGAGTACTCCTCAAGAAAAGCTGCAAATCTTCCAATTCATATAGACACCCAAGCAGGTCGTGGCCCAGAATCCAAGAGTGAGACTTACCCTGTGAAAGAAGGTGGCGACAATATTAATAATATTGTTGTGGTGCAGCGTGCTTATCAGTTCTTTAAAATCTCCATGGGTCTTATTATAGATCTTCTTTTTGACTTTCATCAGCGGAACGAGAGCCGTGCCTATTTTCTTCAGATAACCCAAGAAGAGGCTTATACGTTAATCGCAACTGAGCTCAACTTTATGTATGAAGCTCTTTACACCAAAGCTTATGTGGTGCATTCTGTGCGGGGATACATTTTAAGGGCTATATCATTTGGAACGGTTTGTATAGCCCTTGGTTTCTTCTATAAGTTGGACAAACATGGCTTCCATAGGTTTGATGTTAAAGTTACCTATTCCTTGCTCTTGGGTGCCATAGCCTTGGATTTAATAGCTATTTCGATGCTTATCCTATCTGATTGGACTGCCACATTTGGCCAGCGGTACCAGAAGTCTTGTTTAGCCACTATTCTCATGCACAAGCACCTCAATTGCTTGAAGACATGTTGGTGGTCTACTACTGAAAAAACAACAAAGTGGAAGATTTTATTTGGAAGATGGTCTGAATCTGTCTCTACATTCAGCTTCATAGATTATTCTCTCAAAGAATGCGGAAGAGTGCCACCTACAGTTATGGACTATGTAGGCTTTTTGGGCACTAAAATAATCAAAATCATGGGGCTCAAGGATATTCGAGATGAGTTGAAATATAGATCCAGCAAGAAACTCTCCAAGGAATTATGGAATTTCATTTTTAAAGAGTTGAAAAGTAAATCGGAGGATGCAGGTCGTCCTGAAACTGGCAGCAAAATATGTTCAGCAAGAGGAGAGTGGATTCTTAAGAAGAACTATGGGGATAGGCCAGAGTATGACAAATTAAGATCGCGACTTTATCTTGTCGATGTTACTTATGACCGAAGCATTCTGTTGTGGCACATTGCAACCGAGTTGTACTATTACACTGATGCGAACAATGCTGGTGATGATCATCGTGAGTTTAGTAAGATATTGTCTGATTATATGTTATATCTCTTAGTTATGCAGCCTGCTTTGATGTCTTCTGTAGCGGGAATCGGTCAGAAGAGATTTTCTGATACTTCTGCAGAAGCCAAACAATTCTTTGAAAAAAGGGCACTGGGATCGGCTGCAAAACCAGAATACGCTTGTGGAAAACTGCTCGATGTAAATACAGACATTAAACCCGTTGATGTCAAAGGGAATAGAAGCAAGTCTGTATTGTTTGATGCATGTATTCTGGCAAGGGAGCTGAAGGATTTGGGATGGAAATTGATAAGTGAAGTGTGGGTGGAATTACTATCACATGCTGCAAGTCATTGCAGACCAAGTAGCCATGCCCAATTGCTGAGTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTATTAATGGCTCATTTTGGCATAGCAGAGCAGTTCCAAGTGCTAGAGGTCCAAGCAAGAGTAAAACTGATAGTTGAAAAGCCACCGGGTGAATTCTTTTCTTGGTCTGATGAAAGCCACCAGGGTGAAAAGCCACATAACACAAAACAAATACTCAAACCCAAACCGTCGACATCGCTTGGGCTAAAATCAAACGCCTCTAGCAGATGGTCTCAAATCGAAGGCGTCCTGGCTTTGGTAGATGGCCTCAAATCGAACCCATCCCGGCTCCAGCAGATGGTCTCAAATCAAACCCAAACCTCTGACCTCGCCTGGGTTCAAAGTGAACGCCTCCAACACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

800

Amino Acids

90.88

Weight (kDa)

9.08

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 419 7.2e-90 Domain of unknown function (DUF4220)
DUF594 PF04578 647 - 694 3.9e-22 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 626, 1436
Acc65I GGTACC 1 cut(s) 1104
AccB1I GGYRCC 2 cut(s) 1031, 1104
AccB7I CCANNNNNTGG 2 cut(s) 385, 2335
AccI GTMKAC 2 cut(s) 1175, 2228
AccII CGCG 1 cut(s) 1546
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 7 cut(s) 12, 258, 784, 907, 1102, 1272, 1727
AclWI GGATC 2 cut(s) 1368, 1807
AcoI YGGCCR 3 cut(s) 350, 440, 1096
AcsI RAATTY 2 cut(s) 1405, 2150
AcuI CTGAAG 2 cut(s) 794, 1949
AcvI CACGTG 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 2283
AdeI CACNNNGTG 1 cut(s) 2147
AfaI GTAC 6 cut(s) 398, 546, 575, 587, 1106, 1613
AfiI CCNNNNNNNGG 7 cut(s) 385, 554, 826, 1460, 2293, 2335, 2371
AflII CTTAAG 2 cut(s) 514, 1499
AflIII ACRYGT 2 cut(s) 491, 1163
AgsI TTSAA 6 cut(s) 1159, 1366, 1426, 1784, 2135, 2378
AjnI CCWGG 6 cut(s) 297, 378, 463, 2178, 2286, 2369
AleI CACNNNNGTG 2 cut(s) 300, 2181
Alw21I GWGCWC 2 cut(s) 118, 855
Alw26I GTCTC 7 cut(s) 243, 374, 527, 656, 1237, 2274, 2344
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 2 cut(s) 1368, 1807
Ama87I CYCGRG 1 cut(s) 420
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 8 cut(s) 96, 259, 350, 440, 643, 1096, 1520, 2302
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 8 cut(s) 191, 222, 602, 715, 1464, 1702, 1805, 1985
ApoI RAATTY 2 cut(s) 1405, 2150
AseI ATTAAT 3 cut(s) 212, 696, 2063
Asp718I GGTACC 1 cut(s) 1104
AspS9I GGNCC 4 cut(s) 305, 557, 644, 2107
AsuC2I CCSGG 3 cut(s) 65, 2145, 2323
AsuHPI GGTGA 4 cut(s) 160, 1652, 2159, 2195
AvaI CYCGRG 1 cut(s) 420
AvaII GGWCC 3 cut(s) 305, 557, 2107
BaeGI GKGCMC 4 cut(s) 118, 159, 1319, 1795
BalI TGGCCA 3 cut(s) 352, 442, 1098
BanI GGYRCC 2 cut(s) 1031, 1104
BanII GRGCYC 3 cut(s) 280, 855, 1344
BbrPI CACGTG 1 cut(s) 494
BbsI GAAGAC 3 cut(s) 394, 1166, 1710
Bbv12I GWGCWC 2 cut(s) 118, 855
BbvI GCAGC 8 cut(s) 178, 234, 589, 727, 1476, 1714, 1792, 1972
BccI CCATC 6 cut(s) 1212, 1935, 2259, 2294, 2326, 2329
BceAI ACGGC 1 cut(s) 780
BcgI CGANNNNNNTGC 6 cut(s) 401, 435, 779, 813, 1782, 1816
BciT130I CCWGG 6 cut(s) 299, 380, 465, 2180, 2288, 2371
BciVI GTATCC 1 cut(s) 905
BclI TGATCA 1 cut(s) 1645
BcnI CCSGG 3 cut(s) 65, 2145, 2323
BcoDI GTCTC 7 cut(s) 243, 374, 527, 656, 1237, 2274, 2344
BfaI CTAG 2 cut(s) 2102, 2259
BfmI CTRYAG 3 cut(s) 1287, 1722, 1761
BfrI CTTAAG 2 cut(s) 514, 1499
BfuAI ACCTGC 2 cut(s) 626, 1436
BfuI GTATCC 1 cut(s) 905
BglII AGATCT 1 cut(s) 760
BisI GCNGC 9 cut(s) 192, 223, 259, 603, 716, 1465, 1703, 1806, 1986
BlsI GCNGC 9 cut(s) 193, 224, 260, 604, 717, 1466, 1704, 1807, 1987
BmcAI AGTACT 1 cut(s) 587
Bme1390I CCNGG 9 cut(s) 65, 299, 380, 465, 2145, 2180, 2288, 2323, 2371
Bme18I GGWCC 3 cut(s) 305, 557, 2107
BmeT110I CYCGRG 1 cut(s) 420
BmgT120I GGNCC 4 cut(s) 305, 557, 644, 2107
BmiI GGNNCC 5 cut(s) 59, 279, 1033, 1106, 2327
BmrFI CCNGG 9 cut(s) 65, 299, 380, 465, 2145, 2180, 2288, 2323, 2371
BmrI ACTGGG 2 cut(s) 196, 1805
BmsI GCATC 4 cut(s) 1053, 1432, 1615, 1894
BmuI ACTGGG 2 cut(s) 196, 1805
BoxI GACNNNNGTC 1 cut(s) 398
BpiI GAAGAC 3 cut(s) 394, 1166, 1710
BplI GAGNNNNNCTC 2 cut(s) 575, 607
BpmI CTGGAG 1 cut(s) 2312
BpuEI CTTGAG 3 cut(s) 509, 577, 1328
BpuMI CCSGG 3 cut(s) 65, 2145, 2323
BsaAI YACGTR 1 cut(s) 494
BsaBI GATNNNNATC 2 cut(s) 1067, 1647
BsaHI GRCGYC 1 cut(s) 2283
BsaI GGTCTC 3 cut(s) 374, 2274, 2344
BsaJI CCNNGG 9 cut(s) 64, 298, 463, 744, 955, 1041, 1392, 2179, 2370
BsaWI WCCGGW 3 cut(s) 307, 547, 559
BsaXI ACNNNNNCTCC 2 cut(s) 2028, 2058
Bsc4I CCNNNNNNNGG 7 cut(s) 385, 554, 826, 1460, 2293, 2335, 2371
Bse1I ACTGG 4 cut(s) 191, 559, 1465, 1800
Bse3DI GCAATG 3 cut(s) 89, 1596, 1993
Bse8I GATNNNNATC 2 cut(s) 1067, 1647
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 6 cut(s) 299, 380, 465, 2180, 2288, 2371
BseDI CCNNGG 9 cut(s) 64, 298, 463, 744, 955, 1041, 1392, 2179, 2370
BseGI GGATG 4 cut(s) 586, 1447, 1946, 2318
BseJI GATNNNNATC 2 cut(s) 1067, 1647
BseLI CCNNNNNNNGG 7 cut(s) 385, 554, 826, 1460, 2293, 2335, 2371
BseMI GCAATG 3 cut(s) 89, 1596, 1993
BseMII CTCAG 2 cut(s) 840, 2015
BseNI ACTGG 4 cut(s) 191, 559, 1465, 1800
BseRI GAGGAG 2 cut(s) 580, 1500
BseSI GKGCMC 4 cut(s) 118, 159, 1319, 1795
BseXI GCAGC 8 cut(s) 178, 234, 589, 727, 1476, 1714, 1792, 1972
BsgI GTGCAG 1 cut(s) 734
Bsh1236I CGCG 1 cut(s) 1546
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 8 cut(s) 98, 261, 352, 442, 645, 1098, 1522, 2304
BshNI GGYRCC 2 cut(s) 1031, 1104
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 118, 855
BsiHKCI CYCGRG 1 cut(s) 420
BsiSI CCGG 6 cut(s) 64, 308, 548, 560, 2144, 2323
BsiWI CGTACG 1 cut(s) 396
BslI CCNNNNNNNGG 7 cut(s) 385, 554, 826, 1460, 2293, 2335, 2371
BsmAI GTCTC 7 cut(s) 243, 374, 527, 656, 1237, 2274, 2344
BsmI GAATGC 3 cut(s) 898, 1274, 1581
BsnI GGCC 8 cut(s) 98, 261, 352, 442, 645, 1098, 1522, 2304
Bso31I GGTCTC 3 cut(s) 374, 2274, 2344
BsoBI CYCGRG 1 cut(s) 420
Bsp1286I GDGCHC 7 cut(s) 118, 159, 280, 855, 1319, 1344, 1795
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 6 cut(s) 150, 760, 1373, 1541, 1645, 1799
Bsp19I CCATGG 1 cut(s) 744
Bsp68I TCGCGA 1 cut(s) 1546
BspACI CCGC 7 cut(s) 12, 258, 784, 907, 1102, 1272, 1727
BspANI GGCC 8 cut(s) 98, 261, 352, 442, 645, 1098, 1522, 2304
BspCNI CTCAG 2 cut(s) 841, 2016
BspEI TCCGGA 1 cut(s) 307
BspFNI CGCG 1 cut(s) 1546
BspLI GGNNCC 5 cut(s) 59, 279, 1033, 1106, 2327
BspMAI CTGCAG 1 cut(s) 1765
BspMI ACCTGC 2 cut(s) 626, 1436
BspPI GGATC 2 cut(s) 1368, 1807
BspT107I GGYRCC 2 cut(s) 1031, 1104
BspTI CTTAAG 2 cut(s) 514, 1499
BspTNI GGTCTC 3 cut(s) 374, 2274, 2344
BsrDI GCAATG 3 cut(s) 89, 1596, 1993
BsrI ACTGG 4 cut(s) 191, 559, 1465, 1800
BssECI CCNNGG 9 cut(s) 64, 298, 463, 744, 955, 1041, 1392, 2179, 2370
BssMI GATC 6 cut(s) 150, 760, 1373, 1541, 1645, 1799
BssNI GRCGYC 1 cut(s) 2283
BssT1I CCWWGG 4 cut(s) 744, 955, 1041, 1392
Bst2UI CCWGG 6 cut(s) 299, 380, 465, 2180, 2288, 2371
Bst4CI ACNGT 5 cut(s) 34, 242, 943, 1291, 2226
Bst6I CTCTTC 3 cut(s) 819, 1270, 1736
BstACI GRCGYC 1 cut(s) 2283
BstAFI CTTAAG 2 cut(s) 514, 1499
BstAPI GCANNNNNTGC 1 cut(s) 904
BstBAI YACGTR 1 cut(s) 494
BstC8I GCNNGC 7 cut(s) 96, 180, 220, 511, 720, 1100, 1707
BstDEI CTNAG 3 cut(s) 849, 1693, 2024
BstDSI CCRYGG 1 cut(s) 744
BstF5I GGATG 4 cut(s) 586, 1447, 1946, 2318
BstFNI CGCG 1 cut(s) 1546
BstKTI GATC 6 cut(s) 153, 763, 1376, 1544, 1648, 1802
BstMAI GTCTC 7 cut(s) 243, 374, 527, 656, 1237, 2274, 2344
BstMBI GATC 6 cut(s) 150, 760, 1373, 1541, 1645, 1799
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 7 cut(s) 188, 200, 275, 439, 904, 1314, 1634
BstNI CCWGG 6 cut(s) 299, 380, 465, 2180, 2288, 2371
BstNSI RCATGY 3 cut(s) 1167, 1911, 1985
BstPAI GACNNNNGTC 1 cut(s) 398
BstSCI CCNGG 9 cut(s) 63, 297, 378, 463, 2143, 2178, 2286, 2321, 2369
BstSFI CTRYAG 3 cut(s) 1287, 1722, 1761
BstSLI GKGCMC 4 cut(s) 118, 159, 1319, 1795
BstUI CGCG 1 cut(s) 1546
BstV1I GCAGC 8 cut(s) 178, 234, 589, 727, 1476, 1714, 1792, 1972
BstV2I GAAGAC 3 cut(s) 394, 1166, 1710
BstX2I RGATCY 2 cut(s) 760, 1373
BstXI CCANNNNNNTGG 1 cut(s) 1042
BstYI RGATCY 2 cut(s) 760, 1373
BsuI GTATCC 1 cut(s) 905
BsuRI GGCC 8 cut(s) 98, 261, 352, 442, 645, 1098, 1522, 2304
BtgI CCRYGG 1 cut(s) 744
BtgZI GCGATG 1 cut(s) 2217
BtsCI GGATG 4 cut(s) 586, 1447, 1946, 2318
BtsIMutI CAGTG 3 cut(s) 552, 1620, 1793
BtuMI TCGCGA 1 cut(s) 1546
BveI ACCTGC 2 cut(s) 626, 1436
Cac8I GCNNGC 7 cut(s) 96, 180, 220, 511, 720, 1100, 1707
Cfr13I GGNCC 4 cut(s) 305, 557, 644, 2107
CseI GACGC 1 cut(s) 2272
Csp6I GTAC 6 cut(s) 397, 545, 574, 586, 1105, 1612
CviQI GTAC 6 cut(s) 397, 545, 574, 586, 1105, 1612
DdeI CTNAG 3 cut(s) 849, 1693, 2024
DpnI GATC 6 cut(s) 152, 762, 1375, 1543, 1647, 1801
DpnII GATC 6 cut(s) 150, 760, 1373, 1541, 1645, 1799
DraI TTTAAA 2 cut(s) 736, 1417
DraIII CACNNNGTG 1 cut(s) 2147
EaeI YGGCCR 3 cut(s) 350, 440, 1096
Eam1104I CTCTTC 3 cut(s) 819, 1270, 1736
EarI CTCTTC 3 cut(s) 819, 1270, 1736
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 4 cut(s) 744, 955, 1041, 1392
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 3 cut(s) 280, 855, 1344
Eco31I GGTCTC 3 cut(s) 374, 2274, 2344
Eco47I GGWCC 3 cut(s) 305, 557, 2107
Eco53kI GAGCTC 1 cut(s) 853
Eco57I CTGAAG 2 cut(s) 794, 1949
Eco72I CACGTG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 420
EcoICRI GAGCTC 1 cut(s) 853
EcoRI GAATTC 1 cut(s) 2150
EcoRII CCWGG 6 cut(s) 297, 378, 463, 2178, 2286, 2369
EcoT14I CCWWGG 4 cut(s) 744, 955, 1041, 1392
EcoT22I ATGCAT 1 cut(s) 1909
EcoT38I GRGCYC 3 cut(s) 280, 855, 1344
ErhI CCWWGG 4 cut(s) 744, 955, 1041, 1392
FauI CCCGC 2 cut(s) 900, 1720
FbaI TGATCA 1 cut(s) 1645
FblI GTMKAC 2 cut(s) 1175, 2228
Fnu4HI GCNGC 9 cut(s) 192, 223, 259, 603, 716, 1465, 1703, 1806, 1986
FokI GGATG 4 cut(s) 593, 1454, 1953, 2305
FriOI GRGCYC 3 cut(s) 280, 855, 1344
Fsp4HI GCNGC 9 cut(s) 192, 223, 259, 603, 716, 1465, 1703, 1806, 1986
FspBI CTAG 2 cut(s) 2102, 2259
GluI GCNGC 9 cut(s) 192, 223, 259, 603, 716, 1465, 1703, 1806, 1986
GsuI CTGGAG 1 cut(s) 2312
HaeIII GGCC 8 cut(s) 98, 261, 352, 442, 645, 1098, 1522, 2304
HapII CCGG 6 cut(s) 64, 308, 548, 560, 2144, 2323
HgaI GACGC 1 cut(s) 2272
Hin1I GRCGYC 1 cut(s) 2283
HincII GTYRAC 2 cut(s) 417, 2229
HindII GTYRAC 2 cut(s) 417, 2229
HindIII AAGCTT 1 cut(s) 885
HinfI GANTC 5 cut(s) 530, 650, 1226, 1495, 1731
HpaII CCGG 6 cut(s) 64, 308, 548, 560, 2144, 2323
HphI GGTGA 4 cut(s) 160, 1652, 2159, 2195
Hpy166II GTNNAC 5 cut(s) 116, 417, 1176, 2229, 2384
Hpy188III TCNNGA 7 cut(s) 308, 422, 594, 1355, 1454, 1545, 1652
Hpy8I GTNNAC 5 cut(s) 116, 417, 1176, 2229, 2384
Hpy99I CGWCG 1 cut(s) 2230
HpyAV CCTTC 3 cut(s) 674, 1924, 2273
HpyCH4III ACNGT 5 cut(s) 34, 242, 943, 1291, 2226
HpyCH4IV ACGT 4 cut(s) 395, 399, 493, 836
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 200, 275, 439, 904, 1314, 1634
HpyF3I CTNAG 3 cut(s) 849, 1693, 2024
HpySE526I ACGT 4 cut(s) 395, 399, 493, 836
Hsp92I GRCGYC 1 cut(s) 2283
Kpn2I TCCGGA 1 cut(s) 307
KpnI GGTACC 1 cut(s) 1108
Ksp22I TGATCA 1 cut(s) 1645
Kzo9I GATC 6 cut(s) 150, 760, 1373, 1541, 1645, 1799
LmnI GCTCC 3 cut(s) 283, 1924, 2331
Lsp1109I GCAGC 8 cut(s) 178, 234, 589, 727, 1476, 1714, 1792, 1972
LweI GCATC 4 cut(s) 1053, 1432, 1615, 1894
MaeI CTAG 2 cut(s) 2102, 2259
MaeII ACGT 4 cut(s) 395, 399, 493, 836
MaeIII GTNAC 5 cut(s) 291, 391, 1009, 1564, 2044
MalI GATC 6 cut(s) 152, 762, 1375, 1543, 1647, 1801
MboI GATC 6 cut(s) 150, 760, 1373, 1541, 1645, 1799
MfeI CAATTG 2 cut(s) 1150, 2018
MflI RGATCY 2 cut(s) 760, 1373
MhlI GDGCHC 7 cut(s) 118, 159, 280, 855, 1319, 1344, 1795
MlsI TGGCCA 3 cut(s) 352, 442, 1098
MluNI TGGCCA 3 cut(s) 352, 442, 1098
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 1 cut(s) 954
Mox20I TGGCCA 3 cut(s) 352, 442, 1098
Mph1103I ATGCAT 1 cut(s) 1909
MroI TCCGGA 1 cut(s) 307
MscI TGGCCA 3 cut(s) 352, 442, 1098
MslI CAYNNNNRTG 3 cut(s) 300, 1168, 2181
Msp20I TGGCCA 3 cut(s) 352, 442, 1098
MspA1I CMGCKG 2 cut(s) 784, 1102
MspCI CTTAAG 2 cut(s) 514, 1499
MspI CCGG 6 cut(s) 64, 308, 548, 560, 2144, 2323
MspR9I CCNGG 9 cut(s) 65, 299, 380, 465, 2145, 2180, 2288, 2323, 2371
MunI CAATTG 2 cut(s) 1150, 2018
Mva1269I GAATGC 3 cut(s) 898, 1274, 1581
MvaI CCWGG 6 cut(s) 299, 380, 465, 2180, 2288, 2371
MvnI CGCG 1 cut(s) 1546
MwoI GCNNNNNNNGC 7 cut(s) 188, 200, 275, 439, 904, 1314, 1634
NciI CCSGG 3 cut(s) 65, 2145, 2323
NcoI CCATGG 1 cut(s) 744
NdeII GATC 6 cut(s) 150, 760, 1373, 1541, 1645, 1799
NlaIV GGNNCC 5 cut(s) 59, 279, 1033, 1106, 2327
NmeAIII GCCGAG 1 cut(s) 272
NmuCI GTSAC 2 cut(s) 391, 2044
NruI TCGCGA 1 cut(s) 1546
NsiI ATGCAT 1 cut(s) 1909
NspI RCATGY 3 cut(s) 1167, 1911, 1985
OliI CACNNNNGTG 2 cut(s) 300, 2181
PaeR7I CTCGAG 1 cut(s) 420
PceI AGGCCT 1 cut(s) 98
PciI ACATGT 1 cut(s) 1163
PctI GAATGC 3 cut(s) 898, 1274, 1581
PfeI GAWTC 4 cut(s) 650, 1226, 1495, 1731
Pfl23II CGTACG 1 cut(s) 396
PflMI CCANNNNNTGG 2 cut(s) 385, 2335
PkrI GCNGC 9 cut(s) 193, 224, 260, 604, 717, 1466, 1704, 1807, 1987
PleI GAGTC 1 cut(s) 524
PmaCI CACGTG 1 cut(s) 494
PmlI CACGTG 1 cut(s) 494
PpsI GAGTC 1 cut(s) 524
Ppu21I YACGTR 1 cut(s) 494
PscI ACATGT 1 cut(s) 1163
PshAI GACNNNNGTC 1 cut(s) 398
PshBI ATTAAT 3 cut(s) 212, 696, 2063
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 6 cut(s) 297, 378, 463, 2178, 2286, 2369
PspCI CACGTG 1 cut(s) 494
PspGI CCWGG 6 cut(s) 297, 378, 463, 2178, 2286, 2369
PspLI CGTACG 1 cut(s) 396
PspN4I GGNNCC 5 cut(s) 59, 279, 1033, 1106, 2327
PspPI GGNCC 4 cut(s) 305, 557, 644, 2107
PstI CTGCAG 1 cut(s) 1765
PsuI RGATCY 2 cut(s) 760, 1373
RruI TCGCGA 1 cut(s) 1546
RsaI GTAC 6 cut(s) 398, 546, 575, 587, 1106, 1613
RsaNI GTAC 6 cut(s) 397, 545, 574, 586, 1105, 1612
RseI CAYNNNNRTG 3 cut(s) 300, 1168, 2181
SacI GAGCTC 1 cut(s) 855
SalI GTCGAC 1 cut(s) 2227
SatI GCNGC 9 cut(s) 192, 223, 259, 603, 716, 1465, 1703, 1806, 1986
Sau3AI GATC 6 cut(s) 150, 760, 1373, 1541, 1645, 1799
Sau96I GGNCC 4 cut(s) 305, 557, 644, 2107
ScaI AGTACT 1 cut(s) 587
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 9 cut(s) 65, 299, 380, 465, 2145, 2180, 2288, 2323, 2371
SduI GDGCHC 7 cut(s) 118, 159, 280, 855, 1319, 1344, 1795
SfaNI GCATC 4 cut(s) 1053, 1432, 1615, 1894
SfcI CTRYAG 3 cut(s) 1287, 1722, 1761
Sfr274I CTCGAG 1 cut(s) 420
SinI GGWCC 3 cut(s) 305, 557, 2107
SlaI CTCGAG 1 cut(s) 420
SmiMI CAYNNNNRTG 3 cut(s) 300, 1168, 2181
SmlI CTYRAG 6 cut(s) 420, 514, 524, 592, 1343, 1499
SmoI CTYRAG 6 cut(s) 420, 514, 524, 592, 1343, 1499
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 7 cut(s) 12, 258, 784, 907, 1102, 1272, 1727
SspI AATATT 2 cut(s) 694, 703
SspMI CTAG 2 cut(s) 2102, 2259
SstI GAGCTC 1 cut(s) 855
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 9 cut(s) 63, 297, 378, 463, 2143, 2178, 2286, 2321, 2369
StyI CCWWGG 4 cut(s) 744, 955, 1041, 1392
TaaI ACNGT 5 cut(s) 34, 242, 943, 1291, 2226
TaiI ACGT 4 cut(s) 398, 402, 496, 839
TaqI TCGA 8 cut(s) 421, 1061, 1354, 1560, 1839, 2228, 2277, 2312
TaqII GACCGA 2 cut(s) 1590, 1724
TatI WGTACW 3 cut(s) 573, 585, 1611
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 4 cut(s) 650, 1226, 1495, 1731
TscAI CASTG 3 cut(s) 559, 1627, 1800
TseFI GTSAC 2 cut(s) 391, 2044
TseI GCWGC 8 cut(s) 191, 222, 602, 715, 1464, 1702, 1805, 1985
Tsp45I GTSAC 2 cut(s) 391, 2044
TspDTI ATGAA 7 cut(s) 394, 608, 767, 882, 1237, 1399, 2184
TspRI CASTG 3 cut(s) 559, 1627, 1800
Van91I CCANNNNNTGG 2 cut(s) 385, 2335
Vha464I CTTAAG 2 cut(s) 514, 1499
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 3 cut(s) 305, 557, 2107
VspI ATTAAT 3 cut(s) 212, 696, 2063
XapI RAATTY 2 cut(s) 1405, 2150
XceI RCATGY 3 cut(s) 1167, 1911, 1985
XcmI CCANNNNNNNNNTGG 2 cut(s) 638, 889
XhoI CTCGAG 1 cut(s) 420
XmiI GTMKAC 2 cut(s) 1175, 2228
XspI CTAG 2 cut(s) 2102, 2259
ZrmI AGTACT 1 cut(s) 587
Zsp2I ATGCAT 1 cut(s) 1909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.