RchiOBHm_Chr2g0112841

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000662
Physical Location & Seq
Reverse (-)
24885902 .. 24888354
2453 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48625

Sequence Viewer

Length: 2250 bp
ATGGCTTCATTAGCCGCTCCTATTTCTAACAAGGTAAAGAAGATCTGGGATGACTGGAATCTCCGGAGCTTCATCATATTAAGTCTCTCATTGCAGACCTTCTTAATTCTCTTTGCACCCTTGAGAAAGAGAACATCGAGCAAATGGCTAATCGTGCCCATCTGGTTAGCCTACTTGCTCGCTGACTGGTCTGCTAACTTTGCTATCGGACTAATTGCAAGCAGCCAAACGAACACTGATCAAGGTTCCCCAGAGAATGGTAATCTCTTGGCATTCTGGGCTCCCTTTCTTCTTTTGCACCTTGGTGGTCCAGACACCATAATTGCAATTGCTATGGAAGACAATGCGTTGTGGCCAAGGCACTTGCTTGGTCTCATATTCCAGGTTGTAGCTGCAGCGTACGTCTTCGGTATGCAGTTCTCTAACCAAAACAAGCTGCTGTGGCCAACACTCTTACTGTTTGTACCAGCACTTGTCAAGTACGGTGAGCGAACACGTGCTTTATATCTTGCCAGCTTGGAAAGTCTCAAGGAAACCATACTTGCGGTACCGGAACCTGGACCGGATTATGCAAAGTACATGGATGAGTACTCCTCAAGAAAAGCTGCCCATCTTCCAATTAAAATAGAAATTGAAGAAGGTCGTAGCTCAGATTCTAAGAATGTGACTTACCGGCTGGGAGAAGGAGACGTCATTGATAATATTACACTAGTGGAGGGTGCTCATCACTTGTTTCAAATTTCCATGGGTCTTATTATAGATCTTGTGTTCGACTATCATCAGCGCAAAGAGAGCCGTGCCTATTTCATCAAATTAGAATCAGGAGATGCTTTTACATCAATTGCTACTGAGCTCAATTTTATGTATGAAGCTCTGTACACCAAAGCTTATGTGGTACATTCAAAACGGGGATACTTGTTGAGAGCTACATCCTTCATCGCCGTTTGCATAGCCTTTGTTTTCTTCTATAGAATAGAGAAACATGGCTTCCACAGCTTTGATGTTGGAATTACTTATTCCTTGCTTTTTGGTGCCATAGCTCTTGATTCAGTAGCTCTTTCGATGCTGATATTATCTGATTGGACGATCGCTATTGTATACAAGTTCTGCAAAAAGTCTCGTGCAGTCACTGATCTCATGAAGAAGTACCTGGAGTTCAAGAGATCATGGGGACCAACTACTACACCAAAGTGTCTAGAGCGGACCAGAAAGATTTTATTTCGAAGATGGTGTGAATCTATCTCGGGTTTCAGCTTCATAGATTATTCTCTCCGAGAATGTGGAAGATCAGTCCGACTCCGACCTGGGGACCGTTTTGGCCTTGTTTACCTGTACCATAGATTCATCAGATTCTTGGGCCACCGTTACCTTCAAATCATCAGATTCTTTGGCCTCAAGTATCTTCGAGATGAGATGAAGTATAGATCCAGCAAGAGACTTGAAGAGATTTTGTGGAAATTCATTTTTGAAGAGTTGGAAAGTAAATCCGAAGTTGTTGATGATCCCAAAATTGGTAGAGAAATATGTTCGTCCCAAGGGGATTGGGTTCTCCTAAAAAAGTATGAGAAGCATCCGGAGTACAAGAACTTGCTAGCATGCTTTGTTGATGTTACTTATGACCGACGCGTTCTGCTGTGGCATATTGCTACTGAGATCTGCTATTACAGTGTTGATACAGGCACTGGAGGTGATAATCGTAAATACAGTAAGACGTTGTCTGATTATATGCTATATCTCTTAATAATGCAGCCTGCTTTGATGTCCTCTGTAGCGGGAATCGGACAAAAGAGATTTAGAGATACGGTTGCAGAAGCCAATAAATTCTTTAAAAAAAGGGGATTGGGTTCCGATGTGAAACTAGGCGAGGGGAAACTACGCGAGGCTTGTTGCAAACTCCTAGATGTAGATACAGGAGTTAAACCAGTCGATGTCAAAGGGAATAGAAGTAAATCTGTTTTGTTTGATGCATGTATTTTGGCCAGGCAGCTGATGAAATTCGAAGACAAGAAGAAATGGGAATTAATAAGCTCGGTGTGGGTGGAATTGCTGTCATATGCTGCAAGTCATTGCAGAGCACATAGCCACGCGCAATTGCTGACTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTACTGATGGCTCGCTTTGGCATTGAGGAGGACATCCAAGTACTGGAGGTCCAAGCAAGAGTAAAACTGATCATGAACAAGCAACCTGATGATTTCAAGAATAATTGGCGCACAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

749

Amino Acids

86.19

Weight (kDa)

9.15

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 422 4.4e-90 Domain of unknown function (DUF4220)
DUF594 PF04578 667 - 718 7.9e-22 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 693
Acc65I GGTACC 1 cut(s) 547
AccB1I GGYRCC 2 cut(s) 547, 1031
AccB7I CCANNNNNTGG 2 cut(s) 257, 2173
AccBSI CCGCTC 2 cut(s) 17, 1201
AccI GTMKAC 1 cut(s) 1098
AccII CGCG 3 cut(s) 1626, 1878, 2087
AccIII TCCGGA 2 cut(s) 63, 1573
AciI CCGC 4 cut(s) 15, 545, 1201, 1772
AclWI GGATC 2 cut(s) 1419, 1496
AcoI YGGCCR 3 cut(s) 353, 443, 1977
AcsI RAATTY 4 cut(s) 738, 1457, 1820, 1994
AcvI CACGTG 1 cut(s) 497
AcyI GRCGYC 1 cut(s) 690
AfiI CCNNNNNNNGG 5 cut(s) 257, 557, 1306, 1511, 2173
AflIII ACRYGT 2 cut(s) 494, 1624
AgsI TTSAA 8 cut(s) 635, 737, 903, 1159, 1373, 1442, 1469, 2227
AhlI ACTAGT 1 cut(s) 709
AjnI CCWGG 5 cut(s) 381, 556, 1149, 1303, 1979
AleI CACNNNNGTG 2 cut(s) 303, 1189
Alw21I GWGCWC 3 cut(s) 724, 855, 2077
Alw26I GTCTC 6 cut(s) 89, 377, 530, 681, 1122, 1429
AlwI GGATC 2 cut(s) 1419, 1496
AlwNI CAGNNNCTG 2 cut(s) 1130, 1682
Ama87I CYCGRG 1 cut(s) 1243
Aor13HI TCCGGA 2 cut(s) 63, 1573
AoxI GGCC 6 cut(s) 353, 443, 1318, 1357, 1390, 1977
ApeKI GCWGC 8 cut(s) 222, 392, 395, 436, 605, 1747, 1984, 2057
ApoI RAATTY 4 cut(s) 738, 1457, 1820, 1994
AseI ATTAAT 1 cut(s) 2021
Asp718I GGTACC 1 cut(s) 547
AspLEI GCGC 3 cut(s) 786, 2089, 2241
AspS9I GGNCC 7 cut(s) 308, 560, 1172, 1203, 1309, 1357, 2179
AsuHPI GGTGA 2 cut(s) 497, 1700
AsuII TTCGAA 2 cut(s) 1222, 1998
AsuNHI GCTAGC 1 cut(s) 1591
AvaI CYCGRG 1 cut(s) 1243
AvaII GGWCC 6 cut(s) 308, 560, 1172, 1203, 1309, 2179
BaeGI GKGCMC 1 cut(s) 159
BaeI ACNNNNGTAYC 2 cut(s) 1899, 1932
BalI TGGCCA 3 cut(s) 355, 445, 1979
BanI GGYRCC 2 cut(s) 547, 1031
BanII GRGCYC 2 cut(s) 283, 855
BauI CACGAG 1 cut(s) 1119
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 3 cut(s) 345, 397, 2007
Bbv12I GWGCWC 3 cut(s) 724, 855, 2077
BbvI GCAGC 8 cut(s) 234, 379, 407, 423, 592, 1759, 1996, 2044
BccI CCATC 4 cut(s) 167, 618, 1221, 2131
BceAI ACGGC 2 cut(s) 780, 926
BciT130I CCWGG 5 cut(s) 383, 558, 1151, 1305, 1981
BciVI GTATCC 1 cut(s) 905
BclI TGATCA 2 cut(s) 238, 2199
BcoDI GTCTC 6 cut(s) 89, 377, 530, 681, 1122, 1429
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 5 cut(s) 710, 1196, 1592, 1859, 1898
BfmI CTRYAG 3 cut(s) 393, 967, 1767
BfuI GTATCC 1 cut(s) 905
BglII AGATCT 3 cut(s) 42, 760, 1653
BisI GCNGC 9 cut(s) 15, 223, 393, 396, 437, 606, 1748, 1985, 2058
BlsI GCNGC 9 cut(s) 16, 224, 394, 397, 438, 607, 1749, 1986, 2059
BmcAI AGTACT 2 cut(s) 590, 2172
Bme1390I CCNGG 5 cut(s) 383, 558, 1151, 1305, 1981
Bme18I GGWCC 6 cut(s) 308, 560, 1172, 1203, 1309, 2179
BmeT110I CYCGRG 1 cut(s) 1243
BmgT120I GGNCC 7 cut(s) 308, 560, 1172, 1203, 1309, 1357, 2179
BmiI GGNNCC 8 cut(s) 247, 282, 549, 555, 1033, 1173, 1310, 1846
BmrFI CCNGG 5 cut(s) 383, 558, 1151, 1305, 1981
BmsI GCATC 4 cut(s) 817, 1053, 1579, 1954
BmtI GCTAGC 1 cut(s) 1595
BpiI GAAGAC 3 cut(s) 345, 397, 2007
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 3 cut(s) 1172, 1704, 2195
Bpu14I TTCGAA 2 cut(s) 1222, 1998
BpuEI CTTGAG 4 cut(s) 142, 512, 580, 1379
BsaAI YACGTR 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 690
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 5 cut(s) 301, 356, 744, 1304, 1534
BsaWI WCCGGW 4 cut(s) 63, 550, 562, 1573
BsaXI ACNNNNNCTCC 2 cut(s) 2100, 2130
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 557, 1306, 1511, 2173
Bse118I RCCGGY 1 cut(s) 672
Bse1I ACTGG 5 cut(s) 59, 191, 1687, 1922, 2178
Bse3DI GCAATG 2 cut(s) 89, 2065
BseAI TCCGGA 2 cut(s) 63, 1573
BseBI CCWGG 5 cut(s) 383, 558, 1151, 1305, 1981
BseDI CCNNGG 5 cut(s) 301, 356, 744, 1304, 1534
BseGI GGATG 5 cut(s) 55, 589, 929, 1570, 2163
BseLI CCNNNNNNNGG 5 cut(s) 257, 557, 1306, 1511, 2173
BseMI GCAATG 2 cut(s) 89, 2065
BseMII CTCAG 3 cut(s) 663, 840, 1641
BseNI ACTGG 5 cut(s) 59, 191, 1687, 1922, 2178
BseRI GAGGAG 2 cut(s) 583, 2171
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 8 cut(s) 234, 379, 407, 423, 592, 1759, 1996, 2044
BseYI CCCAGC 1 cut(s) 676
BsgI GTGCAG 1 cut(s) 1143
Bsh1236I CGCG 3 cut(s) 1626, 1878, 2087
Bsh1285I CGRYCG 1 cut(s) 1089
BshFI GGCC 6 cut(s) 355, 445, 1320, 1359, 1392, 1979
BshNI GGYRCC 2 cut(s) 547, 1031
BsiEI CGRYCG 1 cut(s) 1089
BsiHKAI GWGCWC 3 cut(s) 724, 855, 2077
BsiHKCI CYCGRG 1 cut(s) 1243
BsiSI CCGG 5 cut(s) 64, 551, 563, 673, 1574
BsiWI CGTACG 1 cut(s) 399
BslFI GGGAC 3 cut(s) 1185, 1322, 1516
BslI CCNNNNNNNGG 5 cut(s) 257, 557, 1306, 1511, 2173
BsmAI GTCTC 6 cut(s) 89, 377, 530, 681, 1122, 1429
BsmBI CGTCTC 1 cut(s) 681
BsmFI GGGAC 3 cut(s) 1185, 1322, 1516
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 6 cut(s) 355, 445, 1320, 1359, 1392, 1979
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 1 cut(s) 1243
Bsp119I TTCGAA 2 cut(s) 1222, 1998
Bsp1286I GDGCHC 5 cut(s) 159, 283, 724, 855, 2077
Bsp13I TCCGGA 2 cut(s) 63, 1573
Bsp1407I TGTACA 1 cut(s) 876
Bsp19I CCATGG 1 cut(s) 744
BspACI CCGC 4 cut(s) 15, 545, 1201, 1772
BspANI GGCC 6 cut(s) 355, 445, 1320, 1359, 1392, 1979
BspCNI CTCAG 3 cut(s) 662, 841, 1642
BspEI TCCGGA 2 cut(s) 63, 1573
BspFNI CGCG 3 cut(s) 1626, 1878, 2087
BspHI TCATGA 2 cut(s) 1137, 2202
BspLI GGNNCC 8 cut(s) 247, 282, 549, 555, 1033, 1173, 1310, 1846
BspMAI CTGCAG 1 cut(s) 397
BspOI GCTAGC 1 cut(s) 1595
BspPI GGATC 2 cut(s) 1419, 1496
BspT104I TTCGAA 2 cut(s) 1222, 1998
BspT107I GGYRCC 2 cut(s) 547, 1031
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 2 cut(s) 17, 1201
BsrDI GCAATG 2 cut(s) 89, 2065
BsrFI RCCGGY 1 cut(s) 672
BsrGI TGTACA 1 cut(s) 876
BsrI ACTGG 5 cut(s) 59, 191, 1687, 1922, 2178
BssAI RCCGGY 1 cut(s) 672
BssECI CCNNGG 5 cut(s) 301, 356, 744, 1304, 1534
BssNAI GTATAC 1 cut(s) 1099
BssNI GRCGYC 1 cut(s) 690
BssSI CACGAG 1 cut(s) 1119
BssT1I CCWWGG 4 cut(s) 301, 356, 744, 1534
Bst1107I GTATAC 1 cut(s) 1099
Bst2BI CACGAG 1 cut(s) 1119
Bst2UI CCWGG 5 cut(s) 383, 558, 1151, 1305, 1981
Bst4CI ACNGT 7 cut(s) 459, 485, 1313, 1364, 1667, 1706, 1804
Bst6I CTCTTC 2 cut(s) 1437, 1464
BstACI GRCGYC 1 cut(s) 690
BstAUI TGTACA 1 cut(s) 876
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 2 cut(s) 1222, 1998
BstC8I GCNNGC 7 cut(s) 180, 220, 514, 1593, 1597, 1752, 2143
BstDEI CTNAG 4 cut(s) 649, 657, 849, 1650
BstDSI CCRYGG 1 cut(s) 744
BstF5I GGATG 5 cut(s) 55, 589, 929, 1570, 2163
BstFNI CGCG 3 cut(s) 1626, 1878, 2087
BstHHI GCGC 3 cut(s) 786, 2089, 2241
BstMAI GTCTC 6 cut(s) 89, 377, 530, 681, 1122, 1429
BstMCI CGRYCG 1 cut(s) 1089
BstMWI GCNNNNNNNGC 7 cut(s) 11, 154, 200, 278, 442, 792, 993
BstNI CCWGG 5 cut(s) 383, 558, 1151, 1305, 1981
BstNSI RCATGY 2 cut(s) 1599, 1971
BstSCI CCNGG 5 cut(s) 381, 556, 1149, 1303, 1979
BstSFI CTRYAG 3 cut(s) 393, 967, 1767
BstSLI GKGCMC 1 cut(s) 159
BstUI CGCG 3 cut(s) 1626, 1878, 2087
BstV1I GCAGC 8 cut(s) 234, 379, 407, 423, 592, 1759, 1996, 2044
BstV2I GAAGAC 3 cut(s) 345, 397, 2007
BstX2I RGATCY 4 cut(s) 42, 760, 1424, 1653
BstYI RGATCY 4 cut(s) 42, 760, 1424, 1653
BstZ17I GTATAC 1 cut(s) 1099
BsuI GTATCC 1 cut(s) 905
BsuRI GGCC 6 cut(s) 355, 445, 1320, 1359, 1392, 1979
BtgI CCRYGG 1 cut(s) 744
BtgZI GCGATG 1 cut(s) 922
BtsCI GGATG 5 cut(s) 55, 589, 929, 1570, 2163
BtsIMutI CAGTG 4 cut(s) 234, 1128, 1672, 1680
Cac8I GCNNGC 7 cut(s) 180, 220, 514, 1593, 1597, 1752, 2143
CaiI CAGNNNCTG 2 cut(s) 1130, 1682
CciI TCATGA 2 cut(s) 1137, 2202
CfoI GCGC 3 cut(s) 786, 2089, 2241
Cfr10I RCCGGY 1 cut(s) 672
Cfr13I GGNCC 7 cut(s) 308, 560, 1172, 1203, 1309, 1357, 2179
CseI GACGC 1 cut(s) 1632
CviAII CATG 8 cut(s) 580, 745, 983, 1138, 1167, 1596, 1968, 2203
DdeI CTNAG 4 cut(s) 649, 657, 849, 1650
DraI TTTAAA 1 cut(s) 1828
EaeI YGGCCR 3 cut(s) 353, 443, 1977
Eam1104I CTCTTC 2 cut(s) 1437, 1464
EarI CTCTTC 2 cut(s) 1437, 1464
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 4 cut(s) 301, 356, 744, 1534
Eco24I GRGCYC 2 cut(s) 283, 855
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 6 cut(s) 308, 560, 1172, 1203, 1309, 2179
Eco53kI GAGCTC 1 cut(s) 853
Eco72I CACGTG 1 cut(s) 497
Eco88I CYCGRG 1 cut(s) 1243
EcoICRI GAGCTC 1 cut(s) 853
EcoRII CCWGG 5 cut(s) 381, 556, 1149, 1303, 1979
EcoT14I CCWWGG 4 cut(s) 301, 356, 744, 1534
EcoT22I ATGCAT 1 cut(s) 1969
EcoT38I GRGCYC 2 cut(s) 283, 855
ErhI CCWWGG 4 cut(s) 301, 356, 744, 1534
Esp3I CGTCTC 1 cut(s) 681
FaeI CATG 8 cut(s) 583, 748, 986, 1141, 1170, 1599, 1971, 2206
FaqI GGGAC 3 cut(s) 1185, 1322, 1516
FatI CATG 8 cut(s) 579, 744, 982, 1137, 1166, 1595, 1967, 2202
FauI CCCGC 1 cut(s) 1765
FauNDI CATATG 1 cut(s) 2053
FbaI TGATCA 2 cut(s) 238, 2199
FblI GTMKAC 1 cut(s) 1098
Fnu4HI GCNGC 9 cut(s) 15, 223, 393, 396, 437, 606, 1748, 1985, 2058
FokI GGATG 5 cut(s) 62, 596, 916, 1557, 2150
FriOI GRGCYC 2 cut(s) 283, 855
Fsp4HI GCNGC 9 cut(s) 15, 223, 393, 396, 437, 606, 1748, 1985, 2058
FspBI CTAG 5 cut(s) 710, 1196, 1592, 1859, 1898
GlaI GCGC 3 cut(s) 785, 2088, 2240
GluI GCNGC 9 cut(s) 15, 223, 393, 396, 437, 606, 1748, 1985, 2058
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 3 cut(s) 1172, 1704, 2195
HaeIII GGCC 6 cut(s) 355, 445, 1320, 1359, 1392, 1979
HapII CCGG 5 cut(s) 64, 551, 563, 673, 1574
HgaI GACGC 1 cut(s) 1632
HhaI GCGC 3 cut(s) 786, 2089, 2241
Hin1I GRCGYC 1 cut(s) 690
Hin1II CATG 8 cut(s) 583, 748, 986, 1141, 1170, 1599, 1971, 2206
Hin6I GCGC 3 cut(s) 784, 2087, 2239
HinP1I GCGC 3 cut(s) 784, 2087, 2239
HindIII AAGCTT 1 cut(s) 885
HpaII CCGG 5 cut(s) 64, 551, 563, 673, 1574
HphI GGTGA 2 cut(s) 497, 1700
Hpy166II GTNNAC 3 cut(s) 879, 1099, 1327
Hpy8I GTNNAC 3 cut(s) 879, 1099, 1327
Hpy99I CGWCG 1 cut(s) 1626
HpyAV CCTTC 5 cut(s) 109, 632, 677, 943, 1379
HpyCH4III ACNGT 7 cut(s) 459, 485, 1313, 1364, 1667, 1706, 1804
HpyCH4IV ACGT 4 cut(s) 402, 496, 690, 1712
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 154, 200, 278, 442, 792, 993
HpyF3I CTNAG 4 cut(s) 649, 657, 849, 1650
HpySE526I ACGT 4 cut(s) 402, 496, 690, 1712
Hsp92I GRCGYC 1 cut(s) 690
Hsp92II CATG 8 cut(s) 583, 748, 986, 1141, 1170, 1599, 1971, 2206
HspAI GCGC 3 cut(s) 784, 2087, 2239
Kpn2I TCCGGA 2 cut(s) 63, 1573
KpnI GGTACC 1 cut(s) 551
Ksp22I TGATCA 2 cut(s) 238, 2199
LmnI GCTCC 3 cut(s) 22, 66, 286
Lsp1109I GCAGC 8 cut(s) 234, 379, 407, 423, 592, 1759, 1996, 2044
LweI GCATC 4 cut(s) 817, 1053, 1579, 1954
MaeI CTAG 5 cut(s) 710, 1196, 1592, 1859, 1898
MaeII ACGT 4 cut(s) 402, 496, 690, 1712
MaeIII GTNAC 6 cut(s) 664, 1126, 1364, 1609, 2116, 2130
MbiI CCGCTC 2 cut(s) 17, 1201
MfeI CAATTG 3 cut(s) 327, 840, 2090
MflI RGATCY 4 cut(s) 42, 760, 1424, 1653
MhlI GDGCHC 5 cut(s) 159, 283, 724, 855, 2077
MlsI TGGCCA 3 cut(s) 355, 445, 1979
MluI ACGCGT 1 cut(s) 1624
MluNI TGGCCA 3 cut(s) 355, 445, 1979
MlyI GAGTC 1 cut(s) 1290
MmeI TCCRAC 4 cut(s) 985, 1318, 1324, 1455
Mox20I TGGCCA 3 cut(s) 355, 445, 1979
Mph1103I ATGCAT 1 cut(s) 1969
MroI TCCGGA 2 cut(s) 63, 1573
MscI TGGCCA 3 cut(s) 355, 445, 1979
MseI TTAA 8 cut(s) 80, 104, 621, 1739, 1827, 1917, 2021, 2248
MslI CAYNNNNRTG 2 cut(s) 303, 1189
Msp20I TGGCCA 3 cut(s) 355, 445, 1979
MspA1I CMGCKG 1 cut(s) 1987
MspI CCGG 5 cut(s) 64, 551, 563, 673, 1574
MspR9I CCNGG 5 cut(s) 383, 558, 1151, 1305, 1981
MunI CAATTG 3 cut(s) 327, 840, 2090
Mva1269I GAATGC 1 cut(s) 272
MvaI CCWGG 5 cut(s) 383, 558, 1151, 1305, 1981
MvnI CGCG 3 cut(s) 1626, 1878, 2087
MwoI GCNNNNNNNGC 7 cut(s) 11, 154, 200, 278, 442, 792, 993
NcoI CCATGG 1 cut(s) 744
NdeI CATATG 1 cut(s) 2053
NheI GCTAGC 1 cut(s) 1591
NlaIII CATG 8 cut(s) 583, 748, 986, 1141, 1170, 1599, 1971, 2206
NlaIV GGNNCC 8 cut(s) 247, 282, 549, 555, 1033, 1173, 1310, 1846
NmuCI GTSAC 3 cut(s) 664, 1126, 2116
NsiI ATGCAT 1 cut(s) 1969
NspI RCATGY 2 cut(s) 1599, 1971
NspV TTCGAA 2 cut(s) 1222, 1998
OliI CACNNNNGTG 2 cut(s) 303, 1189
PaeI GCATGC 1 cut(s) 1599
PagI TCATGA 2 cut(s) 1137, 2202
PctI GAATGC 1 cut(s) 272
PfeI GAWTC 9 cut(s) 58, 653, 818, 1046, 1235, 1341, 1350, 1383, 1776
Pfl23II CGTACG 1 cut(s) 399
PflFI GACNNNGTC 1 cut(s) 1714
PflMI CCANNNNNTGG 2 cut(s) 257, 2173
PkrI GCNGC 9 cut(s) 16, 224, 394, 397, 438, 607, 1749, 1986, 2059
Ple19I CGATCG 1 cut(s) 1089
PleI GAGTC 1 cut(s) 1290
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 1 cut(s) 1290
Ppu21I YACGTR 1 cut(s) 497
PshBI ATTAAT 1 cut(s) 2021
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 5 cut(s) 381, 556, 1149, 1303, 1979
PspCI CACGTG 1 cut(s) 497
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 5 cut(s) 381, 556, 1149, 1303, 1979
PspLI CGTACG 1 cut(s) 399
PspN4I GGNNCC 8 cut(s) 247, 282, 549, 555, 1033, 1173, 1310, 1846
PspPI GGNCC 7 cut(s) 308, 560, 1172, 1203, 1309, 1357, 2179
PsrI GAACNNNNNNTAC 2 cut(s) 1139, 1171
PstI CTGCAG 1 cut(s) 397
PstNI CAGNNNCTG 2 cut(s) 1130, 1682
PsuI RGATCY 4 cut(s) 42, 760, 1424, 1653
PsyI GACNNNGTC 1 cut(s) 1714
PvuI CGATCG 1 cut(s) 1089
PvuII CAGCTG 1 cut(s) 1987
RseI CAYNNNNRTG 2 cut(s) 303, 1189
SacI GAGCTC 1 cut(s) 855
SaqAI TTAA 8 cut(s) 80, 104, 621, 1739, 1827, 1917, 2021, 2248
SatI GCNGC 9 cut(s) 15, 223, 393, 396, 437, 606, 1748, 1985, 2058
Sau96I GGNCC 7 cut(s) 308, 560, 1172, 1203, 1309, 1357, 2179
ScaI AGTACT 2 cut(s) 590, 2172
SchI GAGTC 1 cut(s) 1290
ScrFI CCNGG 5 cut(s) 383, 558, 1151, 1305, 1981
SduI GDGCHC 5 cut(s) 159, 283, 724, 855, 2077
SfaNI GCATC 4 cut(s) 817, 1053, 1579, 1954
SfcI CTRYAG 3 cut(s) 393, 967, 1767
SfuI TTCGAA 2 cut(s) 1222, 1998
SinI GGWCC 6 cut(s) 308, 560, 1172, 1203, 1309, 2179
SmiMI CAYNNNNRTG 2 cut(s) 303, 1189
SmlI CTYRAG 4 cut(s) 121, 527, 595, 1394
SmoI CTYRAG 4 cut(s) 121, 527, 595, 1394
SpeI ACTAGT 1 cut(s) 709
SphI GCATGC 1 cut(s) 1599
SsiI CCGC 4 cut(s) 15, 545, 1201, 1772
SspI AATATT 1 cut(s) 703
SspMI CTAG 5 cut(s) 710, 1196, 1592, 1859, 1898
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 5 cut(s) 381, 556, 1149, 1303, 1979
StyI CCWWGG 4 cut(s) 301, 356, 744, 1534
TaaI ACNGT 7 cut(s) 459, 485, 1313, 1364, 1667, 1706, 1804
TaiI ACGT 4 cut(s) 405, 499, 693, 1715
TaqI TCGA 7 cut(s) 137, 771, 1061, 1222, 1405, 1926, 1998
TaqII GACCGA 1 cut(s) 1635
TatI WGTACW 5 cut(s) 576, 588, 876, 1578, 2170
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 9 cut(s) 58, 653, 818, 1046, 1235, 1341, 1350, 1383, 1776
Tru1I TTAA 8 cut(s) 80, 104, 621, 1739, 1827, 1917, 2021, 2248
Tru9I TTAA 8 cut(s) 80, 104, 621, 1739, 1827, 1917, 2021, 2248
TscAI CASTG 4 cut(s) 241, 1135, 1672, 1687
TseFI GTSAC 3 cut(s) 664, 1126, 2116
TseI GCWGC 8 cut(s) 222, 392, 395, 436, 605, 1747, 1984, 2057
Tsp45I GTSAC 3 cut(s) 664, 1126, 2116
TspRI CASTG 4 cut(s) 241, 1135, 1672, 1687
Tth111I GACNNNGTC 1 cut(s) 1714
Van91I CCANNNNNTGG 2 cut(s) 257, 2173
VpaK11BI GGWCC 6 cut(s) 308, 560, 1172, 1203, 1309, 2179
VspI ATTAAT 1 cut(s) 2021
XapI RAATTY 4 cut(s) 738, 1457, 1820, 1994
XbaI TCTAGA 1 cut(s) 1195
XceI RCATGY 2 cut(s) 1599, 1971
XcmI CCANNNNNNNNNTGG 1 cut(s) 889
XmiI GTMKAC 1 cut(s) 1098
XspI CTAG 5 cut(s) 710, 1196, 1592, 1859, 1898
ZraI GACGTC 1 cut(s) 691
ZrmI AGTACT 2 cut(s) 590, 2172
Zsp2I ATGCAT 1 cut(s) 1969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.