RLG00000017895

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000662
Physical Location & Seq
Reverse (-)
22710939 .. 22713179
2241 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017895

Sequence Viewer

Length: 2121 bp
ATGGTTTCCTCCGCCGTTCCTATTTCTGACACCGTGAAGAAAATATGGGATAAGTGGAACCTCCGGGGCTTCATCTTATTAAGCCTCTCATTGCAGACCTTTTTAATTCTTTGTGCACCCATGAGAAAGAGAACATCAAGCAAATGGGTGATCGTGCCCATTTGGTTAGCTTACTTGCTCGCAGACTGGGCTGCTAACTTTGCCATTGGATTAATTACAAGCAGCCAACTGATAGCAACGGTCGCCGAGATAATGGCGGCCTTTTGTCATTTTGGGCTCCATTTCTTTTGTTACACCTGGGTGGTTTTGGCTGTGACGTACGTCTTCATTATGCAGTCAGCTAGAGAAAACAAGCTATGGTGGCCAACACTGTTGCTAATTTTCCCAGGACTTGTTAAGTATGGCGAGCGAACACGTGCTTTATATCTTGCCAGCTTAAGTAGTCTCAAGGACTCCATACTTGAAGTACCGGACACTGGACCGGATTATGCAAAGTACATGGATGAGTACTCCTCAAGAAAAGTTGCCAATCTTCCAATTCATATAGACACCAAAGCAGGCCGTGGCCCAGAATCCGAGAGTGAGACTTACCCTGAGGAAGAAGGCGGCGACATTGCTACGGTGCAGCGTGCTTATCAATTCTTTAAAATCTCCATGGGTCTTATTATAGATCTTGATTTCGACTTTCCTCAGCGCAACCAGAGCCGTGCCTATTTCAATAAGAGAACATCAGAAGAGGCTTATACATTGATTGCAACTGAGCTCAACTTTATGTATGAAGCTCTTTACACCAAAGCTTATGTGGTGCATTATGTGTGGGGATACGTTTTAAGGGCTATATCATTTGGAACGGTTTGTATTGCCCTTGGTTTCTTCTATAAGTTGGACAAACATGGCTTCCACAGGTTTGATGTTGGAGTTACCTATTCCTTGCTCTTGGGTGCAATAGCCCTTGATTCAATAGCTATTTCGATGCTTATCTTGTCTGATTGGACTGTTGTAATTAGCAAGTGTTGCCAAAATTCTCGTTTAGCCACTATTCTCATGAATAAGCACCTCAATTTGAAGAGATCTTGGTGGTCTACCACTGTAAAAACAGCAAAGTATTTAGAGAGGACCAGGAAGATTTTATTTCGAAGATGGTCTGAATCTGTCTCTGCATTCCGCTTCATAGATTATTCTCTCCAAGAATGTGGAAGAGTGCCACCTACAGTTATGGACCATGTAGGCTTTTTGGGCTCTAAAATAATCAGAATCATGGGCCTCAACGAGGTTGAGATGAAATATAGATCCCGCAAGAAACTCTCCACGGAACTGTGGGAGTTCATTTTTGCAGAGTTGAAAAGAAAATCGGAGGATGCAGTTGAACCTCAAAATGGCGGCAAAATATGTTCAGCAAGAGGGGAGTGGATTTTTAAGAAGTACAATGGGGATAGGCCAGAGTTTGATGAATTAAGATCGCAAGTTTATCTTGGTGATGATGTTACTTATGACAGACGCATTCTGTTGTGGCACATTGCAACCGAGTTGCTCTATTACAATGAGAAGACGGAGAGCAATGCTGGTGATGAGAAGACGGAGAGCAATGCTGGTGATGAGAAGACGGAGAGAAATGCTGGTGATGAGAAGACGGAAAGCAATGCTCGTGATGAGAAGACGGAAAGCAATGCTCGTGATGAGAAGAAGAGCAATCCTAGTGATAGTGCTCATCGAGAGTTTAGTAAGACATTGTCTGATTATATGTTATATCTCTTAGTTATGCAGCCTGATTTGATGTCTTCGAAGCCAAACAATTCTTTGAAAAAAAGGGCACTGGGCTCGGATGCAGAACCAGGAAATGCTTGTAGAAAACTTCTCGAAGTAAATACAGAAATCAAACCCATTGATGTCAAAGGGAATAAAAGCAAGTCTGTATTGTTTGATGCATGTATTCTGGCAAGGGAGCTGAAGAACAGGGAATGGAAATTGATAAGTGAAGTGTGGGTGGAATTACTATCATATGCTGCAAGTCATTGCAGACCAGGTAGCCATGCCCAATTGCTGAGTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTATTAATGGCTCATTTTGGCATAGCAGAGCAGTACCAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

707

Amino Acids

80.84

Weight (kDa)

8.39

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 102 - 393 1.2e-57 Domain of unknown function (DUF4220)
DUF594 PF04578 650 - 700 6e-23 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1082
AciI CCGC 6 cut(s) 12, 257, 606, 1165, 1294, 1380
AclWI GGATC 1 cut(s) 1284
AcoI YGGCCR 1 cut(s) 362
AcsI RAATTY 1 cut(s) 1021
AcuI CTGAAG 1 cut(s) 1967
AcvI CACGTG 1 cut(s) 416
AfaI GTAC 6 cut(s) 320, 468, 497, 509, 1424, 2111
AfiI CCNNNNNNNGG 3 cut(s) 476, 1270, 1376
AflII CTTAAG 1 cut(s) 436
AflIII ACRYGT 1 cut(s) 413
AgsI TTSAA 7 cut(s) 464, 718, 960, 1066, 1342, 1367, 1801
AjnI CCWGG 5 cut(s) 296, 385, 1118, 1831, 2020
AleI CACNNNNGTG 1 cut(s) 299
Alw21I GWGCWC 3 cut(s) 118, 765, 1708
Alw26I GTCTC 3 cut(s) 449, 578, 1159
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 1 cut(s) 1284
AoxI GGCC 6 cut(s) 258, 362, 559, 565, 1261, 1436
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 5 cut(s) 191, 222, 625, 1762, 2003
ApoI RAATTY 1 cut(s) 1021
AseI ATTAAT 2 cut(s) 212, 2081
AspLEI GCGC 1 cut(s) 696
AspS9I GGNCC 5 cut(s) 479, 566, 1116, 1219, 1261
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 5 cut(s) 160, 1487, 1577, 1604, 1631
AsuII TTCGAA 2 cut(s) 1135, 1781
AvaII GGWCC 3 cut(s) 479, 1116, 1219
AxyI CCTNAGG 1 cut(s) 594
BaeGI GKGCMC 3 cut(s) 118, 159, 1813
BalI TGGCCA 1 cut(s) 364
BanII GRGCYC 4 cut(s) 279, 765, 1241, 1820
BauI CACGAG 2 cut(s) 1644, 1671
BbrPI CACGTG 1 cut(s) 416
BbsI GAAGAC 7 cut(s) 316, 1553, 1580, 1607, 1634, 1661, 1770
Bbv12I GWGCWC 3 cut(s) 118, 765, 1708
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 5 cut(s) 178, 234, 637, 1774, 1990
BccI CCATC 1 cut(s) 1134
BceAI ACGGC 2 cut(s) 546, 690
BcgI CGANNNNNNTGC 2 cut(s) 1800, 1834
BciT130I CCWGG 5 cut(s) 298, 387, 1120, 1833, 2022
BciVI GTATCC 1 cut(s) 815
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 3 cut(s) 449, 578, 1159
BfaI CTAG 2 cut(s) 342, 1695
BfmI CTRYAG 1 cut(s) 1209
BfrI CTTAAG 1 cut(s) 436
BfuI GTATCC 1 cut(s) 815
BglII AGATCT 2 cut(s) 670, 1070
BisI GCNGC 8 cut(s) 192, 223, 258, 607, 626, 1381, 1763, 2004
BlsI GCNGC 8 cut(s) 193, 224, 259, 608, 627, 1382, 1764, 2005
BmcAI AGTACT 1 cut(s) 509
Bme1390I CCNGG 6 cut(s) 65, 298, 387, 1120, 1833, 2022
Bme18I GGWCC 3 cut(s) 479, 1116, 1219
BmgT120I GGNCC 5 cut(s) 479, 566, 1116, 1219, 1261
BmiI GGNNCC 2 cut(s) 59, 278
BmrFI CCNGG 6 cut(s) 65, 298, 387, 1120, 1833, 2022
BmrI ACTGGG 2 cut(s) 196, 1823
BmsI GCATC 4 cut(s) 963, 1348, 1813, 1912
BmuI ACTGGG 2 cut(s) 196, 1823
BoxI GACNNNNGTC 1 cut(s) 320
BpiI GAAGAC 7 cut(s) 316, 1553, 1580, 1607, 1634, 1661, 1770
BplI GAGNNNNNCTC 2 cut(s) 497, 529
Bpu10I CCTNAGC 1 cut(s) 690
Bpu14I TTCGAA 2 cut(s) 1135, 1781
BpuEI CTTGAG 2 cut(s) 431, 499
BpuMI CCSGG 1 cut(s) 65
BsaAI YACGTR 1 cut(s) 416
BsaBI GATNNNNATC 1 cut(s) 977
BsaJI CCNNGG 7 cut(s) 64, 297, 385, 562, 654, 865, 1308
BsaWI WCCGGW 2 cut(s) 469, 481
BsaXI ACNNNNNCTCC 2 cut(s) 2046, 2076
Bsc4I CCNNNNNNNGG 3 cut(s) 476, 1270, 1376
Bse1I ACTGG 3 cut(s) 191, 481, 1818
Bse21I CCTNAGG 1 cut(s) 594
Bse3DI GCAATG 8 cut(s) 89, 612, 1515, 1564, 1591, 1645, 1672, 2011
Bse8I GATNNNNATC 1 cut(s) 977
BseBI CCWGG 5 cut(s) 298, 387, 1120, 1833, 2022
BseDI CCNNGG 7 cut(s) 64, 297, 385, 562, 654, 865, 1308
BseGI GGATG 3 cut(s) 508, 1363, 1828
BseJI GATNNNNATC 1 cut(s) 977
BseLI CCNNNNNNNGG 3 cut(s) 476, 1270, 1376
BseMI GCAATG 8 cut(s) 89, 612, 1515, 1564, 1591, 1645, 1672, 2011
BseMII CTCAG 4 cut(s) 585, 704, 750, 2033
BseNI ACTGG 3 cut(s) 191, 481, 1818
BseRI GAGGAG 1 cut(s) 502
BseSI GKGCMC 3 cut(s) 118, 159, 1813
BseXI GCAGC 5 cut(s) 178, 234, 637, 1774, 1990
BsgI GTGCAG 1 cut(s) 644
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 6 cut(s) 260, 364, 561, 567, 1263, 1438
BsiEI CGRYCG 1 cut(s) 243
BsiHKAI GWGCWC 3 cut(s) 118, 765, 1708
BsiSI CCGG 3 cut(s) 64, 470, 482
BsiWI CGTACG 1 cut(s) 318
BslI CCNNNNNNNGG 3 cut(s) 476, 1270, 1376
BsmAI GTCTC 3 cut(s) 449, 578, 1159
BsmI GAATGC 2 cut(s) 1160, 1500
BsnI GGCC 6 cut(s) 260, 364, 561, 567, 1263, 1438
Bsp119I TTCGAA 2 cut(s) 1135, 1781
Bsp1286I GDGCHC 8 cut(s) 118, 159, 279, 765, 1241, 1708, 1813, 1820
Bsp143I GATC 5 cut(s) 150, 670, 1070, 1289, 1457
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 6 cut(s) 12, 257, 606, 1165, 1294, 1380
BspANI GGCC 6 cut(s) 260, 364, 561, 567, 1263, 1438
BspCNI CTCAG 4 cut(s) 586, 703, 751, 2034
BspHI TCATGA 1 cut(s) 1044
BspLI GGNNCC 2 cut(s) 59, 278
BspPI GGATC 1 cut(s) 1284
BspQI GCTCTTC 1 cut(s) 1679
BspT104I TTCGAA 2 cut(s) 1135, 1781
BspTI CTTAAG 1 cut(s) 436
BsrDI GCAATG 8 cut(s) 89, 612, 1515, 1564, 1591, 1645, 1672, 2011
BsrI ACTGG 3 cut(s) 191, 481, 1818
BssECI CCNNGG 7 cut(s) 64, 297, 385, 562, 654, 865, 1308
BssMI GATC 5 cut(s) 150, 670, 1070, 1289, 1457
BssSI CACGAG 2 cut(s) 1644, 1671
BssT1I CCWWGG 2 cut(s) 654, 865
Bst2BI CACGAG 2 cut(s) 1644, 1671
Bst2UI CCWGG 5 cut(s) 298, 387, 1120, 1833, 2022
Bst4CI ACNGT 9 cut(s) 34, 241, 372, 622, 853, 997, 1090, 1213, 1317
Bst6I CTCTTC 4 cut(s) 729, 1061, 1192, 1679
BstAFI CTTAAG 1 cut(s) 436
BstAPI GCANNNNNTGC 1 cut(s) 1014
BstBAI YACGTR 1 cut(s) 416
BstBI TTCGAA 2 cut(s) 1135, 1781
BstC8I GCNNGC 5 cut(s) 180, 407, 433, 559, 630
BstDEI CTNAG 5 cut(s) 594, 690, 759, 1753, 2042
BstDSI CCRYGG 3 cut(s) 562, 654, 1308
BstENI CCTNNNNNAGG 1 cut(s) 1268
BstF5I GGATG 3 cut(s) 508, 1363, 1828
BstHHI GCGC 1 cut(s) 696
BstKTI GATC 5 cut(s) 153, 673, 1073, 1292, 1460
BstMAI GTCTC 3 cut(s) 449, 578, 1159
BstMBI GATC 5 cut(s) 150, 670, 1070, 1289, 1457
BstMCI CGRYCG 1 cut(s) 243
BstMWI GCNNNNNNNGC 7 cut(s) 188, 200, 242, 361, 702, 1014, 1236
BstNI CCWGG 5 cut(s) 298, 387, 1120, 1833, 2022
BstNSI RCATGY 1 cut(s) 1929
BstPAI GACNNNNGTC 1 cut(s) 320
BstSCI CCNGG 6 cut(s) 63, 296, 385, 1118, 1831, 2020
BstSFI CTRYAG 1 cut(s) 1209
BstSLI GKGCMC 3 cut(s) 118, 159, 1813
BstV1I GCAGC 5 cut(s) 178, 234, 637, 1774, 1990
BstV2I GAAGAC 7 cut(s) 316, 1553, 1580, 1607, 1634, 1661, 1770
BstX2I RGATCY 3 cut(s) 670, 1070, 1289
BstXI CCANNNNNNTGG 1 cut(s) 1193
BstYI RGATCY 3 cut(s) 670, 1070, 1289
Bsu36I CCTNAGG 1 cut(s) 594
BsuI GTATCC 1 cut(s) 815
BsuRI GGCC 6 cut(s) 260, 364, 561, 567, 1263, 1438
BtgI CCRYGG 3 cut(s) 562, 654, 1308
BtsCI GGATG 3 cut(s) 508, 1363, 1828
BtsIMutI CAGTG 4 cut(s) 368, 474, 1086, 1811
Cac8I GCNNGC 5 cut(s) 180, 407, 433, 559, 630
CciI TCATGA 1 cut(s) 1044
CfoI GCGC 1 cut(s) 696
Cfr13I GGNCC 5 cut(s) 479, 566, 1116, 1219, 1261
CseI GACGC 1 cut(s) 1506
CsiI ACCWGGT 1 cut(s) 2020
Csp6I GTAC 6 cut(s) 319, 467, 496, 508, 1423, 2110
CviAII CATG 9 cut(s) 121, 499, 655, 893, 1045, 1223, 1258, 1926, 2030
CviQI GTAC 6 cut(s) 319, 467, 496, 508, 1423, 2110
DdeI CTNAG 5 cut(s) 594, 690, 759, 1753, 2042
DpnI GATC 5 cut(s) 152, 672, 1072, 1291, 1459
DpnII GATC 5 cut(s) 150, 670, 1070, 1289, 1457
DraI TTTAAA 1 cut(s) 646
EaeI YGGCCR 1 cut(s) 362
Eam1104I CTCTTC 4 cut(s) 729, 1061, 1192, 1679
EarI CTCTTC 4 cut(s) 729, 1061, 1192, 1679
Ecl136II GAGCTC 1 cut(s) 763
Eco130I CCWWGG 2 cut(s) 654, 865
Eco24I GRGCYC 4 cut(s) 279, 765, 1241, 1820
Eco47I GGWCC 3 cut(s) 479, 1116, 1219
Eco53kI GAGCTC 1 cut(s) 763
Eco57I CTGAAG 1 cut(s) 1967
Eco72I CACGTG 1 cut(s) 416
Eco81I CCTNAGG 1 cut(s) 594
EcoICRI GAGCTC 1 cut(s) 763
EcoNI CCTNNNNNAGG 1 cut(s) 1268
EcoRII CCWGG 5 cut(s) 296, 385, 1118, 1831, 2020
EcoT14I CCWWGG 2 cut(s) 654, 865
EcoT22I ATGCAT 1 cut(s) 1927
EcoT38I GRGCYC 4 cut(s) 279, 765, 1241, 1820
ErhI CCWWGG 2 cut(s) 654, 865
FaeI CATG 9 cut(s) 124, 502, 658, 896, 1048, 1226, 1261, 1929, 2033
FalI AAGNNNNNCTT 2 cut(s) 1455, 1487
FatI CATG 9 cut(s) 120, 498, 654, 892, 1044, 1222, 1257, 1925, 2029
FauI CCCGC 1 cut(s) 1301
FauNDI CATATG 1 cut(s) 1999
FblI GTMKAC 1 cut(s) 1082
Fnu4HI GCNGC 8 cut(s) 192, 223, 258, 607, 626, 1381, 1763, 2004
FokI GGATG 3 cut(s) 515, 1370, 1835
FriOI GRGCYC 4 cut(s) 279, 765, 1241, 1820
Fsp4HI GCNGC 8 cut(s) 192, 223, 258, 607, 626, 1381, 1763, 2004
FspBI CTAG 2 cut(s) 342, 1695
GlaI GCGC 1 cut(s) 695
GluI GCNGC 8 cut(s) 192, 223, 258, 607, 626, 1381, 1763, 2004
HaeIII GGCC 6 cut(s) 260, 364, 561, 567, 1263, 1438
HapII CCGG 3 cut(s) 64, 470, 482
HgaI GACGC 1 cut(s) 1506
HhaI GCGC 1 cut(s) 696
Hin1II CATG 9 cut(s) 124, 502, 658, 896, 1048, 1226, 1261, 1929, 2033
Hin6I GCGC 1 cut(s) 694
HinP1I GCGC 1 cut(s) 694
HindIII AAGCTT 1 cut(s) 795
HinfI GANTC 5 cut(s) 452, 572, 956, 1148, 1254
HpaII CCGG 3 cut(s) 64, 470, 482
HphI GGTGA 5 cut(s) 160, 1487, 1577, 1604, 1631
Hpy166II GTNNAC 2 cut(s) 116, 1083
Hpy188I TCNGA 9 cut(s) 28, 577, 733, 988, 1147, 1253, 1354, 1735, 1822
Hpy188III TCNNGA 7 cut(s) 516, 674, 1045, 1646, 1673, 1712, 1856
Hpy8I GTNNAC 2 cut(s) 116, 1083
HpyAV CCTTC 1 cut(s) 596
HpyCH4III ACNGT 9 cut(s) 34, 241, 372, 622, 853, 997, 1090, 1213, 1317
HpyCH4IV ACGT 4 cut(s) 317, 321, 415, 825
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 200, 242, 361, 702, 1014, 1236
HpyF3I CTNAG 5 cut(s) 594, 690, 759, 1753, 2042
HpySE526I ACGT 4 cut(s) 317, 321, 415, 825
Hsp92II CATG 9 cut(s) 124, 502, 658, 896, 1048, 1226, 1261, 1929, 2033
HspAI GCGC 1 cut(s) 694
Kzo9I GATC 5 cut(s) 150, 670, 1070, 1289, 1457
LguI GCTCTTC 1 cut(s) 1679
LmnI GCTCC 2 cut(s) 282, 1942
Lsp1109I GCAGC 5 cut(s) 178, 234, 637, 1774, 1990
LweI GCATC 4 cut(s) 963, 1348, 1813, 1912
MabI ACCWGGT 1 cut(s) 2020
MaeI CTAG 2 cut(s) 342, 1695
MaeII ACGT 4 cut(s) 317, 321, 415, 825
MaeIII GTNAC 5 cut(s) 290, 313, 919, 1483, 2062
MalI GATC 5 cut(s) 152, 672, 1072, 1291, 1459
MboI GATC 5 cut(s) 150, 670, 1070, 1289, 1457
MfeI CAATTG 1 cut(s) 2036
MflI RGATCY 3 cut(s) 670, 1070, 1289
MhlI GDGCHC 8 cut(s) 118, 159, 279, 765, 1241, 1708, 1813, 1820
MlsI TGGCCA 1 cut(s) 364
MluNI TGGCCA 1 cut(s) 364
MlyI GAGTC 1 cut(s) 446
MmeI TCCRAC 2 cut(s) 864, 895
Mox20I TGGCCA 1 cut(s) 364
Mph1103I ATGCAT 1 cut(s) 1927
MscI TGGCCA 1 cut(s) 364
MslI CAYNNNNRTG 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 364
MspCI CTTAAG 1 cut(s) 436
MspI CCGG 3 cut(s) 64, 470, 482
MspR9I CCNGG 6 cut(s) 65, 298, 387, 1120, 1833, 2022
MunI CAATTG 1 cut(s) 2036
Mva1269I GAATGC 2 cut(s) 1160, 1500
MvaI CCWGG 5 cut(s) 298, 387, 1120, 1833, 2022
MwoI GCNNNNNNNGC 7 cut(s) 188, 200, 242, 361, 702, 1014, 1236
NciI CCSGG 1 cut(s) 65
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1999
NdeII GATC 5 cut(s) 150, 670, 1070, 1289, 1457
NlaIII CATG 9 cut(s) 124, 502, 658, 896, 1048, 1226, 1261, 1929, 2033
NlaIV GGNNCC 2 cut(s) 59, 278
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 2 cut(s) 313, 2062
NsiI ATGCAT 1 cut(s) 1927
NspI RCATGY 1 cut(s) 1929
NspV TTCGAA 2 cut(s) 1135, 1781
OliI CACNNNNGTG 1 cut(s) 299
PagI TCATGA 1 cut(s) 1044
PciSI GCTCTTC 1 cut(s) 1679
PctI GAATGC 2 cut(s) 1160, 1500
PfeI GAWTC 4 cut(s) 572, 956, 1148, 1254
Pfl23II CGTACG 1 cut(s) 318
PflFI GACNNNGTC 1 cut(s) 1729
PkrI GCNGC 8 cut(s) 193, 224, 259, 608, 627, 1382, 1764, 2005
PleI GAGTC 1 cut(s) 446
PmaCI CACGTG 1 cut(s) 416
PmlI CACGTG 1 cut(s) 416
PpsI GAGTC 1 cut(s) 446
Ppu21I YACGTR 1 cut(s) 416
PshAI GACNNNNGTC 1 cut(s) 320
PshBI ATTAAT 2 cut(s) 212, 2081
Psp124BI GAGCTC 1 cut(s) 765
Psp6I CCWGG 5 cut(s) 296, 385, 1118, 1831, 2020
PspCI CACGTG 1 cut(s) 416
PspGI CCWGG 5 cut(s) 296, 385, 1118, 1831, 2020
PspLI CGTACG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 59, 278
PspPI GGNCC 5 cut(s) 479, 566, 1116, 1219, 1261
PsuI RGATCY 3 cut(s) 670, 1070, 1289
PsyI GACNNNGTC 1 cut(s) 1729
RsaI GTAC 6 cut(s) 320, 468, 497, 509, 1424, 2111
RsaNI GTAC 6 cut(s) 319, 467, 496, 508, 1423, 2110
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 765
SapI GCTCTTC 1 cut(s) 1679
SatI GCNGC 8 cut(s) 192, 223, 258, 607, 626, 1381, 1763, 2004
Sau3AI GATC 5 cut(s) 150, 670, 1070, 1289, 1457
Sau96I GGNCC 5 cut(s) 479, 566, 1116, 1219, 1261
ScaI AGTACT 1 cut(s) 509
SchI GAGTC 1 cut(s) 446
ScrFI CCNGG 6 cut(s) 65, 298, 387, 1120, 1833, 2022
SduI GDGCHC 8 cut(s) 118, 159, 279, 765, 1241, 1708, 1813, 1820
SexAI ACCWGGT 1 cut(s) 2020
SfaNI GCATC 4 cut(s) 963, 1348, 1813, 1912
SfcI CTRYAG 1 cut(s) 1209
SfuI TTCGAA 2 cut(s) 1135, 1781
SinI GGWCC 3 cut(s) 479, 1116, 1219
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 3 cut(s) 436, 446, 514
SmoI CTYRAG 3 cut(s) 436, 446, 514
SsiI CCGC 6 cut(s) 12, 257, 606, 1165, 1294, 1380
SspMI CTAG 2 cut(s) 342, 1695
SstI GAGCTC 1 cut(s) 765
StyD4I CCNGG 6 cut(s) 63, 296, 385, 1118, 1831, 2020
StyI CCWWGG 2 cut(s) 654, 865
TaaI ACNGT 9 cut(s) 34, 241, 372, 622, 853, 997, 1090, 1213, 1317
TaiI ACGT 4 cut(s) 320, 324, 418, 828
TaqI TCGA 6 cut(s) 681, 971, 1135, 1711, 1781, 1857
TatI WGTACW 3 cut(s) 495, 507, 1422
TauI GCSGC 3 cut(s) 260, 609, 1383
TfiI GAWTC 4 cut(s) 572, 956, 1148, 1254
TscAI CASTG 4 cut(s) 375, 481, 1093, 1818
TseFI GTSAC 2 cut(s) 313, 2062
TseI GCWGC 5 cut(s) 191, 222, 625, 1762, 2003
Tsp45I GTSAC 2 cut(s) 313, 2062
TspDTI ATGAA 9 cut(s) 61, 316, 530, 792, 1061, 1159, 1295, 1315, 1464
TspGWI ACGGA 6 cut(s) 1325, 1565, 1592, 1619, 1646, 1673
TspRI CASTG 4 cut(s) 375, 481, 1093, 1818
Tth111I GACNNNGTC 1 cut(s) 1729
Vha464I CTTAAG 1 cut(s) 436
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 3 cut(s) 479, 1116, 1219
VspI ATTAAT 2 cut(s) 212, 2081
XagI CCTNNNNNAGG 1 cut(s) 1268
XapI RAATTY 1 cut(s) 1021
XceI RCATGY 1 cut(s) 1929
XcmI CCANNNNNNNNNTGG 1 cut(s) 799
XmiI GTMKAC 1 cut(s) 1082
XspI CTAG 2 cut(s) 342, 1695
ZrmI AGTACT 1 cut(s) 509
Zsp2I ATGCAT 1 cut(s) 1927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.