Rroxscaffold_6G00399620

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000662
Physical Location & Seq
Reverse (-)
21593474 .. 21595303
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00399620.1

Sequence Viewer

Length: 1830 bp
ATGGGTTTATTAGCCGCTCCTATTTCTAGCAACGTCAAGAAGATCTGGGACAAGTGGAATCTCCGGGGCTTCATCATATTAAGTCTCACATTGCAGGCCTTCTTAATTCTCTTTGCACCCTTGAGAAAGCGAACATCGAGCAAATGGGTAACCGTGCCCATCTGGTTAGCTTACTTGCTCGCTGACTGGGCTGCTAACTTTGCTATCGGATTAATTGCAAGCAGCCAAACGAACATTGATGAAGGCTCCCCAGAGAATGGTAATCTCATGGCATTCTGGGCTCCCTTCCTTCTTTTGCACCTAGGTGGTCCAGATACCATAATTGCAATTGCTATGGAAGATAATGCATTGTGGCCAAGGCACTTGCTTGGTCTCATATTCCAGGCTGTAGCCGCACTGTACGTCTTGTGTATGCAGTTCTCTCACCAAAACAAGCTACTGTGGCCAACACTCTTGCTGTTTGTACCAGGACTTGTCAAGTATGGCGAGCGAACACGTGCTTTATATCTTGCCAGCTTGGAAACTCTCAAGGAAACCAGGTTTGCAGTACAGGAACCTCGACTGGATTATGCAAAGTACATGGATGAGTACTCCTCAAGAAAAGCTGCTCATCTTCCAATTAAATTAGAAATCGTACAAGGTCGTAGCTCAGAACCAGAGTCAAAGAAATTGCCTGACCGACTGAATAAAGAAGACGTACGCGTTGATGATATTGAACTAGTGAAGGGTGCTCATCACTTGTTTCAAATCTCCATGGGACTTATTATAGATCTTGTGTTCGACAAAAATCAGCGCATCGAGACTCGTGAGTATTTCAAGACATTAACATCAGAAGATGCTTTTACATCAATTGCGACTGAGCTCAATTTTATGTATGAAGCTCTGTACACCAAAGTTTCTGTGGCACATTCTGGATGGGGATACGTTCTAAGGGCTACATCCTTCATCGTGGTTTGCGTAGCCCTTGTTTTCTTCTTTAGATTAGAGAAACATGGCTTTCACAGGTTTGATGTTGGAATTACTTATTCCTTGCTTTTTGGTGCCATAGCTCTTGACTCAATAGCTTTTTTGATGCTGATAATATCTGATTGGGCGATCGCTGTTGTATACAAGTTCCGTGAAGAACTCCCTAAAGAGTCTGGTGTAGTCACTCATCTCGTGGAGAAGTACCTGGAGTTTAAGAGATCATGGGGACCAACTACTCTGGAGCGGACCAGAAAGATTTTATTTCGAAGATGGTGTGAATCTGTCTCCGGTTTCAGCTTCATAGATTATTCTCTCCGAGAATGTGGAAGAGTCAGATTTTTGGGCCACCTGTACCTCAGAATCATCATATTCCTGGGCCTCAAGTATCATCAAGATGAGCTCAAGTATCGATCTAGCAAGAGACTGGATAAAAAGCTGTGGAATTTCATTTTTAATGAGTTGAAAACTAAATCCAAGAAAGTAGATGATAAGCTTCCAGACATTGGTAGGGAAATATGTTCGTCCCAGGGGGATTGGGTTCTCCGAAATAAGTATAAGGAGCATTTGGAGTACACTAACTTACGATCGTGCTTTGATGGTGTTGGTTATGACCGACGCGTTCTGATGTGGCATATTGCTACTGAGATTTGCTTTCACAGAGATGTTAATACAGATGTTGGTATGAGCAATGAGCATCCTGACTCTCGTGACTTTAGTAAGACGCTGTCTGAGTACATGCTATATCTCTTAATTATGCAGCCTGCTTTGATGTACTCTGTAGCGGGATTCGGACAAAAGAGATTTAGAGATACAGTTGCCGAAGCCAAACAATTCTTTAAAAAAGGAGATTGGGATCCGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

609

Amino Acids

70.58

Weight (kDa)

8.97

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 425 4.7e-89 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1040
AccB7I CCANNNNNTGG 2 cut(s) 257, 1469
AccBSI CCGCTC 2 cut(s) 17, 1210
AccI GTMKAC 1 cut(s) 1107
AccII CGCG 2 cut(s) 702, 1584
AciI CCGC 4 cut(s) 15, 393, 1210, 1748
AclWI GGATC 2 cut(s) 1814, 1827
AcoI YGGCCR 2 cut(s) 353, 443
AcsI RAATTY 1 cut(s) 1408
AcvI CACGTG 1 cut(s) 497
AfiI CCNNNNNNNGG 2 cut(s) 257, 1469
AflIII ACRYGT 3 cut(s) 494, 700, 1582
AgsI TTSAA 4 cut(s) 716, 746, 817, 1429
AhlI ACTAGT 1 cut(s) 718
AjnI CCWGG 6 cut(s) 381, 466, 536, 1170, 1338, 1491
AleI CACNNNNGTG 1 cut(s) 303
Alw21I GWGCWC 3 cut(s) 733, 864, 1368
Alw26I GTCTC 5 cut(s) 89, 377, 794, 1255, 1381
AlwI GGATC 2 cut(s) 1814, 1827
AoxI GGCC 5 cut(s) 96, 353, 443, 1309, 1342
ApeKI GCWGC 4 cut(s) 191, 222, 605, 1723
ApoI RAATTY 1 cut(s) 1408
AseI ATTAAT 1 cut(s) 212
AsiSI GCGATCGC 1 cut(s) 1098
AspA2I CCTAGG 1 cut(s) 301
AspLEI GCGC 1 cut(s) 795
AspS9I GGNCC 5 cut(s) 308, 1193, 1212, 1309, 1342
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 416
AsuII TTCGAA 1 cut(s) 1231
AvaII GGWCC 3 cut(s) 308, 1193, 1212
AvrII CCTAGG 1 cut(s) 301
BaeGI GKGCMC 1 cut(s) 159
BalI TGGCCA 2 cut(s) 355, 445
BamHI GGATCC 1 cut(s) 1819
BanI GGYRCC 1 cut(s) 1040
BanII GRGCYC 3 cut(s) 283, 864, 1368
BauI CACGAG 3 cut(s) 804, 1157, 1671
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 699
Bbv12I GWGCWC 3 cut(s) 733, 864, 1368
BbvI GCAGC 4 cut(s) 178, 234, 592, 1735
BccI CCATC 4 cut(s) 167, 909, 1230, 1556
BciT130I CCWGG 6 cut(s) 383, 468, 538, 1172, 1340, 1493
BciVI GTATCC 1 cut(s) 914
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 5 cut(s) 89, 377, 794, 1255, 1381
BcuI ACTAGT 1 cut(s) 718
BfaI CTAG 4 cut(s) 27, 302, 719, 1380
BfmI CTRYAG 2 cut(s) 387, 1743
BfuI GTATCC 1 cut(s) 914
BglII AGATCT 2 cut(s) 42, 769
BisI GCNGC 6 cut(s) 15, 192, 223, 393, 606, 1724
BlnI CCTAGG 1 cut(s) 301
BlsI GCNGC 6 cut(s) 16, 193, 224, 394, 607, 1725
BmcAI AGTACT 1 cut(s) 590
Bme1390I CCNGG 7 cut(s) 65, 383, 468, 538, 1172, 1340, 1493
Bme18I GGWCC 3 cut(s) 308, 1193, 1212
BmgT120I GGNCC 5 cut(s) 308, 1193, 1212, 1309, 1342
BmiI GGNNCC 6 cut(s) 247, 282, 555, 1042, 1194, 1821
BmrFI CCNGG 7 cut(s) 65, 383, 468, 538, 1172, 1340, 1493
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 4 cut(s) 804, 826, 1062, 1669
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 1 cut(s) 699
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 2 cut(s) 1193, 1226
Bpu14I TTCGAA 1 cut(s) 1231
BpuEI CTTGAG 5 cut(s) 142, 512, 580, 1331, 1352
BpuMI CCSGG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 1375
BsaAI YACGTR 1 cut(s) 497
BsaBI GATNNNNATC 1 cut(s) 1818
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 7 cut(s) 64, 301, 356, 753, 1339, 1491, 1492
BsaWI WCCGGW 1 cut(s) 1253
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 1469
Bse1I ACTGG 3 cut(s) 191, 567, 1395
Bse3DI GCAATG 2 cut(s) 89, 1660
Bse8I GATNNNNATC 1 cut(s) 1818
BseBI CCWGG 6 cut(s) 383, 468, 538, 1172, 1340, 1493
BseCI ATCGAT 1 cut(s) 1375
BseDI CCNNGG 7 cut(s) 64, 301, 356, 753, 1339, 1491, 1492
BseGI GGATG 4 cut(s) 589, 920, 938, 1660
BseJI GATNNNNATC 1 cut(s) 1818
BseLI CCNNNNNNNGG 2 cut(s) 257, 1469
BseMI GCAATG 2 cut(s) 89, 1660
BseMII CTCAG 5 cut(s) 663, 849, 1336, 1599, 1686
BseNI ACTGG 3 cut(s) 191, 567, 1395
BseRI GAGGAG 1 cut(s) 583
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 4 cut(s) 178, 234, 592, 1735
Bsh1236I CGCG 2 cut(s) 702, 1584
Bsh1285I CGRYCG 2 cut(s) 1098, 1553
BshFI GGCC 5 cut(s) 98, 355, 445, 1311, 1344
BshNI GGYRCC 1 cut(s) 1040
BshVI ATCGAT 1 cut(s) 1375
BsiEI CGRYCG 2 cut(s) 1098, 1553
BsiHKAI GWGCWC 3 cut(s) 733, 864, 1368
BsiSI CCGG 2 cut(s) 64, 1254
BsiWI CGTACG 1 cut(s) 697
BslFI GGGAC 4 cut(s) 62, 771, 1206, 1474
BslI CCNNNNNNNGG 2 cut(s) 257, 1469
BsmAI GTCTC 5 cut(s) 89, 377, 794, 1255, 1381
BsmFI GGGAC 4 cut(s) 62, 771, 1206, 1474
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 5 cut(s) 98, 355, 445, 1311, 1344
Bso31I GGTCTC 1 cut(s) 377
Bsp119I TTCGAA 1 cut(s) 1231
Bsp1286I GDGCHC 5 cut(s) 159, 283, 733, 864, 1368
Bsp1407I TGTACA 1 cut(s) 885
Bsp143I GATC 7 cut(s) 42, 769, 1095, 1184, 1376, 1550, 1819
Bsp19I CCATGG 1 cut(s) 753
BspACI CCGC 4 cut(s) 15, 393, 1210, 1748
BspANI GGCC 5 cut(s) 98, 355, 445, 1311, 1344
BspCNI CTCAG 5 cut(s) 662, 850, 1335, 1600, 1687
BspDI ATCGAT 1 cut(s) 1375
BspFNI CGCG 2 cut(s) 702, 1584
BspLI GGNNCC 6 cut(s) 247, 282, 555, 1042, 1194, 1821
BspPI GGATC 2 cut(s) 1814, 1827
BspT104I TTCGAA 1 cut(s) 1231
BspT107I GGYRCC 1 cut(s) 1040
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 2 cut(s) 17, 1210
BsrDI GCAATG 2 cut(s) 89, 1660
BsrGI TGTACA 1 cut(s) 885
BsrI ACTGG 3 cut(s) 191, 567, 1395
BssECI CCNNGG 7 cut(s) 64, 301, 356, 753, 1339, 1491, 1492
BssMI GATC 7 cut(s) 42, 769, 1095, 1184, 1376, 1550, 1819
BssNAI GTATAC 1 cut(s) 1108
BssSI CACGAG 3 cut(s) 804, 1157, 1671
BssT1I CCWWGG 3 cut(s) 301, 356, 753
Bst1107I GTATAC 1 cut(s) 1108
Bst2BI CACGAG 3 cut(s) 804, 1157, 1671
Bst2UI CCWGG 6 cut(s) 383, 468, 538, 1172, 1340, 1493
Bst4CI ACNGT 4 cut(s) 154, 399, 441, 1780
Bst6I CTCTTC 1 cut(s) 1288
BstAUI TGTACA 1 cut(s) 885
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 1 cut(s) 1231
BstC8I GCNNGC 6 cut(s) 96, 180, 220, 488, 514, 1728
BstDEI CTNAG 6 cut(s) 649, 858, 929, 1322, 1608, 1695
BstDSI CCRYGG 1 cut(s) 753
BstEII GGTNACC 1 cut(s) 148
BstF5I GGATG 4 cut(s) 589, 920, 938, 1660
BstFNI CGCG 2 cut(s) 702, 1584
BstHHI GCGC 1 cut(s) 795
BstKTI GATC 7 cut(s) 45, 772, 1098, 1187, 1379, 1553, 1822
BstMAI GTCTC 5 cut(s) 89, 377, 794, 1255, 1381
BstMBI GATC 7 cut(s) 42, 769, 1095, 1184, 1376, 1550, 1819
BstMCI CGRYCG 2 cut(s) 1098, 1553
BstMWI GCNNNNNNNGC 5 cut(s) 188, 200, 278, 392, 442
BstNI CCWGG 6 cut(s) 383, 468, 538, 1172, 1340, 1493
BstNSI RCATGY 1 cut(s) 1705
BstPI GGTNACC 1 cut(s) 148
BstSCI CCNGG 7 cut(s) 63, 381, 466, 536, 1170, 1338, 1491
BstSFI CTRYAG 2 cut(s) 387, 1743
BstSLI GKGCMC 1 cut(s) 159
BstUI CGCG 2 cut(s) 702, 1584
BstV1I GCAGC 4 cut(s) 178, 234, 592, 1735
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 3 cut(s) 42, 769, 1819
BstYI RGATCY 3 cut(s) 42, 769, 1819
BstZ17I GTATAC 1 cut(s) 1108
Bsu15I ATCGAT 1 cut(s) 1375
BsuI GTATCC 1 cut(s) 914
BsuRI GGCC 5 cut(s) 98, 355, 445, 1311, 1344
BsuTUI ATCGAT 1 cut(s) 1375
BtgI CCRYGG 1 cut(s) 753
BtsCI GGATG 4 cut(s) 589, 920, 938, 1660
BtsIMutI CAGTG 1 cut(s) 395
Cac8I GCNNGC 6 cut(s) 96, 180, 220, 488, 514, 1728
CfoI GCGC 1 cut(s) 795
Cfr13I GGNCC 5 cut(s) 308, 1193, 1212, 1309, 1342
ClaI ATCGAT 1 cut(s) 1375
CseI GACGC 2 cut(s) 1590, 1696
CsiI ACCWGGT 1 cut(s) 536
CviAII CATG 6 cut(s) 268, 580, 754, 992, 1188, 1702
DdeI CTNAG 6 cut(s) 649, 858, 929, 1322, 1608, 1695
DpnI GATC 7 cut(s) 44, 771, 1097, 1186, 1378, 1552, 1821
DpnII GATC 7 cut(s) 42, 769, 1095, 1184, 1376, 1550, 1819
DraI TTTAAA 1 cut(s) 1804
EaeI YGGCCR 2 cut(s) 353, 443
Eam1104I CTCTTC 1 cut(s) 1288
EarI CTCTTC 1 cut(s) 1288
Ecl136II GAGCTC 2 cut(s) 862, 1366
Eco130I CCWWGG 3 cut(s) 301, 356, 753
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 3 cut(s) 283, 864, 1368
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 3 cut(s) 308, 1193, 1212
Eco53kI GAGCTC 2 cut(s) 862, 1366
Eco72I CACGTG 1 cut(s) 497
Eco91I GGTNACC 1 cut(s) 148
EcoICRI GAGCTC 2 cut(s) 862, 1366
EcoO65I GGTNACC 1 cut(s) 148
EcoRII CCWGG 6 cut(s) 381, 466, 536, 1170, 1338, 1491
EcoT14I CCWWGG 3 cut(s) 301, 356, 753
EcoT22I ATGCAT 1 cut(s) 349
EcoT38I GRGCYC 3 cut(s) 283, 864, 1368
ErhI CCWWGG 3 cut(s) 301, 356, 753
FaeI CATG 6 cut(s) 271, 583, 757, 995, 1191, 1705
FaqI GGGAC 4 cut(s) 62, 771, 1206, 1474
FatI CATG 6 cut(s) 267, 579, 753, 991, 1187, 1701
FauI CCCGC 1 cut(s) 1741
FblI GTMKAC 1 cut(s) 1107
Fnu4HI GCNGC 6 cut(s) 15, 192, 223, 393, 606, 1724
FokI GGATG 4 cut(s) 596, 925, 927, 1647
FriOI GRGCYC 3 cut(s) 283, 864, 1368
Fsp4HI GCNGC 6 cut(s) 15, 192, 223, 393, 606, 1724
FspBI CTAG 4 cut(s) 27, 302, 719, 1380
GlaI GCGC 1 cut(s) 794
GluI GCNGC 6 cut(s) 15, 192, 223, 393, 606, 1724
GsuI CTGGAG 2 cut(s) 1193, 1226
HaeIII GGCC 5 cut(s) 98, 355, 445, 1311, 1344
HapII CCGG 2 cut(s) 64, 1254
HgaI GACGC 2 cut(s) 1590, 1696
HhaI GCGC 1 cut(s) 795
Hin1II CATG 6 cut(s) 271, 583, 757, 995, 1191, 1705
Hin6I GCGC 1 cut(s) 793
HinP1I GCGC 1 cut(s) 793
HindIII AAGCTT 1 cut(s) 1457
HpaII CCGG 2 cut(s) 64, 1254
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 3 cut(s) 888, 1108, 1539
Hpy8I GTNNAC 3 cut(s) 888, 1108, 1539
Hpy99I CGWCG 1 cut(s) 1584
HpyAV CCTTC 6 cut(s) 109, 236, 295, 299, 718, 952
HpyCH4III ACNGT 4 cut(s) 154, 399, 441, 1780
HpyCH4IV ACGT 5 cut(s) 33, 402, 496, 696, 924
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 200, 278, 392, 442
HpyF3I CTNAG 6 cut(s) 649, 858, 929, 1322, 1608, 1695
HpySE526I ACGT 5 cut(s) 33, 402, 496, 696, 924
Hsp92II CATG 6 cut(s) 271, 583, 757, 995, 1191, 1705
HspAI GCGC 1 cut(s) 793
Kzo9I GATC 7 cut(s) 42, 769, 1095, 1184, 1376, 1550, 1819
LmnI GCTCC 5 cut(s) 22, 251, 286, 1207, 1525
Lsp1109I GCAGC 4 cut(s) 178, 234, 592, 1735
LweI GCATC 4 cut(s) 804, 826, 1062, 1669
MabI ACCWGGT 1 cut(s) 536
MaeI CTAG 4 cut(s) 27, 302, 719, 1380
MaeII ACGT 5 cut(s) 33, 402, 496, 696, 924
MaeIII GTNAC 3 cut(s) 148, 1147, 1673
MalI GATC 7 cut(s) 44, 771, 1097, 1186, 1378, 1552, 1821
MbiI CCGCTC 2 cut(s) 17, 1210
MboI GATC 7 cut(s) 42, 769, 1095, 1184, 1376, 1550, 1819
MboII GAAGA 9 cut(s) 52, 350, 605, 704, 845, 964, 1133, 1245, 1305
MfeI CAATTG 2 cut(s) 327, 849
MflI RGATCY 3 cut(s) 42, 769, 1819
MhlI GDGCHC 5 cut(s) 159, 283, 733, 864, 1368
MlsI TGGCCA 2 cut(s) 355, 445
MluI ACGCGT 2 cut(s) 700, 1582
MluNI TGGCCA 2 cut(s) 355, 445
MlyI GAGTC 6 cut(s) 668, 796, 1049, 1145, 1305, 1661
MmeI TCCRAC 1 cut(s) 994
MnlI CCTC 4 cut(s) 567, 604, 1331, 1355
Mox20I TGGCCA 2 cut(s) 355, 445
Mph1103I ATGCAT 1 cut(s) 349
MscI TGGCCA 2 cut(s) 355, 445
MslI CAYNNNNRTG 3 cut(s) 303, 1359, 1626
Msp20I TGGCCA 2 cut(s) 355, 445
MspI CCGG 2 cut(s) 64, 1254
MspR9I CCNGG 7 cut(s) 65, 383, 468, 538, 1172, 1340, 1493
MunI CAATTG 2 cut(s) 327, 849
Mva1269I GAATGC 1 cut(s) 272
MvaI CCWGG 6 cut(s) 383, 468, 538, 1172, 1340, 1493
MvnI CGCG 2 cut(s) 702, 1584
MwoI GCNNNNNNNGC 5 cut(s) 188, 200, 278, 392, 442
NciI CCSGG 1 cut(s) 65
NcoI CCATGG 1 cut(s) 753
NdeII GATC 7 cut(s) 42, 769, 1095, 1184, 1376, 1550, 1819
NlaIII CATG 6 cut(s) 271, 583, 757, 995, 1191, 1705
NlaIV GGNNCC 6 cut(s) 247, 282, 555, 1042, 1194, 1821
NmuCI GTSAC 2 cut(s) 1147, 1673
NsiI ATGCAT 1 cut(s) 349
NspI RCATGY 1 cut(s) 1705
NspV TTCGAA 1 cut(s) 1231
OliI CACNNNNGTG 1 cut(s) 303
PasI CCCWGGG 1 cut(s) 1492
PceI AGGCCT 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 954
PctI GAATGC 1 cut(s) 272
PfeI GAWTC 4 cut(s) 58, 1244, 1326, 1752
Pfl23II CGTACG 1 cut(s) 697
PflFI GACNNNGTC 1 cut(s) 1690
PflMI CCANNNNNTGG 2 cut(s) 257, 1469
PkrI GCNGC 6 cut(s) 16, 193, 224, 394, 607, 1725
Ple19I CGATCG 2 cut(s) 1098, 1553
PleI GAGTC 6 cut(s) 667, 796, 1049, 1144, 1304, 1661
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 6 cut(s) 667, 796, 1049, 1144, 1304, 1661
Ppu21I YACGTR 1 cut(s) 497
PshBI ATTAAT 1 cut(s) 212
Psp124BI GAGCTC 2 cut(s) 864, 1368
Psp6I CCWGG 6 cut(s) 381, 466, 536, 1170, 1338, 1491
PspCI CACGTG 1 cut(s) 497
PspEI GGTNACC 1 cut(s) 148
PspGI CCWGG 6 cut(s) 381, 466, 536, 1170, 1338, 1491
PspLI CGTACG 1 cut(s) 697
PspN4I GGNNCC 6 cut(s) 247, 282, 555, 1042, 1194, 1821
PspPI GGNCC 5 cut(s) 308, 1193, 1212, 1309, 1342
PsuI RGATCY 3 cut(s) 42, 769, 1819
PsyI GACNNNGTC 1 cut(s) 1690
PvuI CGATCG 2 cut(s) 1098, 1553
RgaI GCGATCGC 1 cut(s) 1098
RseI CAYNNNNRTG 3 cut(s) 303, 1359, 1626
SacI GAGCTC 2 cut(s) 864, 1368
SatI GCNGC 6 cut(s) 15, 192, 223, 393, 606, 1724
Sau3AI GATC 7 cut(s) 42, 769, 1095, 1184, 1376, 1550, 1819
Sau96I GGNCC 5 cut(s) 308, 1193, 1212, 1309, 1342
ScaI AGTACT 1 cut(s) 590
SchI GAGTC 6 cut(s) 668, 796, 1049, 1145, 1305, 1661
ScrFI CCNGG 7 cut(s) 65, 383, 468, 538, 1172, 1340, 1493
SduI GDGCHC 5 cut(s) 159, 283, 733, 864, 1368
SexAI ACCWGGT 1 cut(s) 536
SfaAI GCGATCGC 1 cut(s) 1098
SfaNI GCATC 4 cut(s) 804, 826, 1062, 1669
SfcI CTRYAG 2 cut(s) 387, 1743
SfuI TTCGAA 1 cut(s) 1231
SgfI GCGATCGC 1 cut(s) 1098
SinI GGWCC 3 cut(s) 308, 1193, 1212
SmiMI CAYNNNNRTG 3 cut(s) 303, 1359, 1626
SmlI CTYRAG 5 cut(s) 121, 527, 595, 1346, 1367
SmoI CTYRAG 5 cut(s) 121, 527, 595, 1346, 1367
SpeI ACTAGT 1 cut(s) 718
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 4 cut(s) 15, 393, 1210, 1748
SspMI CTAG 4 cut(s) 27, 302, 719, 1380
SstI GAGCTC 2 cut(s) 864, 1368
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 7 cut(s) 63, 381, 466, 536, 1170, 1338, 1491
StyI CCWWGG 3 cut(s) 301, 356, 753
TaaI ACNGT 4 cut(s) 154, 399, 441, 1780
TaiI ACGT 5 cut(s) 36, 405, 499, 699, 927
TaqI TCGA 6 cut(s) 137, 559, 780, 798, 1231, 1375
TaqII GACCGA 2 cut(s) 693, 1593
TatI WGTACW 7 cut(s) 547, 576, 588, 885, 1536, 1698, 1737
TauI GCSGC 2 cut(s) 17, 395
TfiI GAWTC 4 cut(s) 58, 1244, 1326, 1752
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 2 cut(s) 1147, 1673
TseI GCWGC 4 cut(s) 191, 222, 605, 1723
Tsp45I GTSAC 2 cut(s) 1147, 1673
TspDTI ATGAA 6 cut(s) 61, 255, 891, 934, 1255, 1402
TspGWI ACGGA 1 cut(s) 1106
TspRI CASTG 1 cut(s) 402
Tth111I GACNNNGTC 1 cut(s) 1690
Van91I CCANNNNNTGG 2 cut(s) 257, 1469
VpaK11BI GGWCC 3 cut(s) 308, 1193, 1212
VspI ATTAAT 1 cut(s) 212
XapI RAATTY 1 cut(s) 1408
XceI RCATGY 1 cut(s) 1705
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XmaJI CCTAGG 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 1107
XspI CTAG 4 cut(s) 27, 302, 719, 1380
ZrmI AGTACT 1 cut(s) 590
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.