Rorug04G0417700

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000662
Physical Location & Seq
Forward (+)
57872746 .. 57876916
4171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0417700.1

Sequence Viewer

Length: 1029 bp
ATGTTGGGGGGCAACAATGCTAATTCCATGCTACCTGTTTTCGTGGATGAAAATCGCTTCCCCTATCCAACAAATGCCACAAATCAGCTGCAGTTGTTTGGTAATGTACCAGCTGGATGTAATGTTGATCCTGTAAATTATTTTGGAACTGAGCATATCACTCCCATGCTTCGGCCTAATAAACGAACCAGGGAAATTGAAGATATCTCAAGACAGCAGAAGCTTCAAATTTCTTTGAACTACAATGTCTGTCAAGATGAAGCTGATCGCTCAGCTAGCATTCCAAACCCGAACCATGTATCGACAGGTTTAAGGCTATCGTATGATGATGATGAGCGCAACTCATCTGTTACATCTGCTAGTGGAAGCATGCCAGCAGCACCGTCAATGATTTTATCCCTAGGGGACAATATTAGGACTGAGCTTGATCGGCAGAAGGAAGAATTTGATCAGTACATCAAAATTCAGGAGGAACACTTGGCAAAGGGGGTAAGAGACATGAAGCAGAGACATATGGCTTCTTTCCTTACCGCTATAGAGAAAGGTGTAGGCAAAAAGCTAAGGGAGAAAGACTTGGAAATTGAGACCATGAACCGCAAGAACCGAGAATTGGTCGATAAAATAAGACAGGTAGCTGTCGAAGCCCAGAATTGGCATTACAGAGCAAAGTACAATGAGTCAGTTGTGAATGTGCTGAAGAGCAACCTCCAGCAAGCAATTTCACAGGGTGCTGACCTAGGGAAGGAGGGTTTTGGAGACAGTGAAGTTGATGACGCTGCCTCATACATTGATCCGACTAACTACCTGGCCATTCAAGGTGGGCCTGCAAAGGCCGTATCCAAAAATTACCTAGGGTTGAAGGAGCAAATGGATTGCAGAGCATGCAAAGCATGCCGAGCAAAGGAGGTGTCTATCTTGTTGATGCCTTGTAGGCACCTGTGTTTATGTAAGGACTGTGATGAGTTCATCAGTGTTTGCCCCGTATGCGAGTCAATGAAAACGGCTAGTTTCCAAGTATACTTGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

342

Amino Acids

38.35

Weight (kDa)

5.95

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 293 - 332 1.1e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 933
AccI GTMKAC 1 cut(s) 1017
AciI CCGC 2 cut(s) 531, 595
AclWI GGATC 2 cut(s) 122, 785
AcoI YGGCCR 1 cut(s) 807
AcsI RAATTY 3 cut(s) 228, 443, 462
AcuI CTGAAG 1 cut(s) 716
AdeI CACNNNGTG 1 cut(s) 728
AfaI GTAC 3 cut(s) 108, 455, 671
AfiI CCNNNNNNNGG 5 cut(s) 171, 610, 651, 742, 901
AgsI TTSAA 5 cut(s) 200, 227, 238, 815, 859
AjnI CCWGG 2 cut(s) 188, 804
AjuI GAANNNNNNNTTGG 2 cut(s) 593, 625
AluBI AGCT 8 cut(s) 88, 113, 223, 263, 275, 424, 559, 635
AluI AGCT 8 cut(s) 88, 113, 223, 263, 275, 424, 559, 635
Alw26I GTCTC 4 cut(s) 489, 502, 578, 750
AlwI GGATC 2 cut(s) 122, 785
AoxI GGCC 4 cut(s) 173, 807, 821, 831
ApeKI GCWGC 3 cut(s) 88, 377, 776
ApoI RAATTY 3 cut(s) 228, 443, 462
ArsI GACNNNNNNTTYG 2 cut(s) 893, 925
AspA2I CCTAGG 3 cut(s) 400, 736, 850
AspLEI GCGC 1 cut(s) 339
AspS9I GGNCC 1 cut(s) 821
AsuNHI GCTAGC 1 cut(s) 275
AvrII CCTAGG 3 cut(s) 400, 736, 850
BalI TGGCCA 1 cut(s) 809
BanI GGYRCC 1 cut(s) 933
BbvI GCAGC 3 cut(s) 75, 389, 763
BceAI ACGGC 2 cut(s) 818, 1017
BciT130I CCWGG 2 cut(s) 190, 806
BciVI GTATCC 1 cut(s) 847
BclI TGATCA 1 cut(s) 448
BcoDI GTCTC 4 cut(s) 489, 502, 578, 750
BfaI CTAG 6 cut(s) 276, 360, 401, 737, 851, 1005
BfmI CTRYAG 2 cut(s) 89, 534
BfuI GTATCC 1 cut(s) 847
BglI GCCNNNNNGGC 1 cut(s) 931
BisI GCNGC 3 cut(s) 89, 378, 777
BlnI CCTAGG 3 cut(s) 400, 736, 850
BlpI GCTNAGC 1 cut(s) 271
BlsI GCNGC 3 cut(s) 90, 379, 778
Bme1390I CCNGG 2 cut(s) 190, 806
BmgT120I GGNCC 1 cut(s) 821
BmiI GGNNCC 1 cut(s) 935
BmrFI CCNGG 2 cut(s) 190, 806
BmsI GCATC 1 cut(s) 912
BmtI GCTAGC 1 cut(s) 279
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 1 cut(s) 692
Bpu10I CCTNAGC 1 cut(s) 560
Bpu1102I GCTNAGC 1 cut(s) 271
BpuEI CTTGAG 1 cut(s) 193
BsaBI GATNNNNATC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 578
BsaJI CCNNGG 4 cut(s) 189, 400, 736, 850
Bsc4I CCNNNNNNNGG 5 cut(s) 171, 610, 651, 742, 901
Bse8I GATNNNNATC 1 cut(s) 51
BseBI CCWGG 2 cut(s) 190, 806
BseDI CCNNGG 4 cut(s) 189, 400, 736, 850
BseGI GGATG 2 cut(s) 52, 122
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 5 cut(s) 171, 610, 651, 742, 901
BseMII CTCAG 3 cut(s) 141, 285, 411
BseXI GCAGC 3 cut(s) 75, 389, 763
BshFI GGCC 4 cut(s) 175, 809, 823, 833
BshNI GGYRCC 1 cut(s) 933
BslFI GGGAC 1 cut(s) 419
BslI CCNNNNNNNGG 5 cut(s) 171, 610, 651, 742, 901
BsmAI GTCTC 4 cut(s) 489, 502, 578, 750
BsmFI GGGAC 1 cut(s) 419
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 4 cut(s) 175, 809, 823, 833
Bso31I GGTCTC 1 cut(s) 578
Bsp143I GATC 5 cut(s) 127, 265, 427, 448, 790
Bsp1720I GCTNAGC 1 cut(s) 271
BspACI CCGC 2 cut(s) 531, 595
BspANI GGCC 4 cut(s) 175, 809, 823, 833
BspCNI CTCAG 3 cut(s) 142, 284, 412
BspLI GGNNCC 1 cut(s) 935
BspMAI CTGCAG 1 cut(s) 93
BspOI GCTAGC 1 cut(s) 279
BspPI GGATC 2 cut(s) 122, 785
BspQI GCTCTTC 1 cut(s) 692
BspT107I GGYRCC 1 cut(s) 933
BspTNI GGTCTC 1 cut(s) 578
BssECI CCNNGG 4 cut(s) 189, 400, 736, 850
BssMI GATC 5 cut(s) 127, 265, 427, 448, 790
BssNAI GTATAC 1 cut(s) 1018
BssT1I CCWWGG 3 cut(s) 400, 736, 850
Bst1107I GTATAC 1 cut(s) 1018
Bst2UI CCWGG 2 cut(s) 190, 806
Bst4CI ACNGT 3 cut(s) 384, 761, 956
Bst6I CTCTTC 1 cut(s) 692
BstAPI GCANNNNNTGC 2 cut(s) 882, 891
BstC8I GCNNGC 7 cut(s) 277, 371, 375, 714, 825, 883, 892
BstDEI CTNAG 4 cut(s) 150, 271, 420, 560
BstENI CCTNNNNNAGG 1 cut(s) 740
BstF5I GGATG 2 cut(s) 52, 122
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 5 cut(s) 130, 268, 430, 451, 793
BstMAI GTCTC 4 cut(s) 489, 502, 578, 750
BstMBI GATC 5 cut(s) 127, 265, 427, 448, 790
BstMWI GCNNNNNNNGC 9 cut(s) 276, 430, 641, 882, 887, 891, 896, 931, 984
BstNI CCWGG 2 cut(s) 190, 806
BstNSI RCATGY 3 cut(s) 373, 885, 894
BstSCI CCNGG 2 cut(s) 188, 804
BstSFI CTRYAG 2 cut(s) 89, 534
BstV1I GCAGC 3 cut(s) 75, 389, 763
BstZ17I GTATAC 1 cut(s) 1018
BsuI GTATCC 1 cut(s) 847
BsuRI GGCC 4 cut(s) 175, 809, 823, 833
BtsCI GGATG 2 cut(s) 52, 122
BtsIMutI CAGTG 2 cut(s) 766, 976
Cac8I GCNNGC 7 cut(s) 277, 371, 375, 714, 825, 883, 892
CfoI GCGC 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 821
CseI GACGC 1 cut(s) 782
Csp6I GTAC 3 cut(s) 107, 454, 670
CviAII CATG 8 cut(s) 28, 166, 296, 370, 499, 589, 882, 891
CviQI GTAC 3 cut(s) 107, 454, 670
DdeI CTNAG 4 cut(s) 150, 271, 420, 560
DpnI GATC 5 cut(s) 129, 267, 429, 450, 792
DpnII GATC 5 cut(s) 127, 265, 427, 448, 790
DraIII CACNNNGTG 1 cut(s) 728
EaeI YGGCCR 1 cut(s) 807
Eam1104I CTCTTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 692
Eco130I CCWWGG 3 cut(s) 400, 736, 850
Eco31I GGTCTC 1 cut(s) 578
Eco32I GATATC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 716
EcoNI CCTNNNNNAGG 1 cut(s) 740
EcoRII CCWGG 2 cut(s) 188, 804
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 3 cut(s) 400, 736, 850
ErhI CCWWGG 3 cut(s) 400, 736, 850
FaeI CATG 8 cut(s) 31, 169, 299, 373, 502, 592, 885, 894
FaqI GGGAC 1 cut(s) 419
FatI CATG 8 cut(s) 27, 165, 295, 369, 498, 588, 881, 890
FauNDI CATATG 1 cut(s) 513
FbaI TGATCA 1 cut(s) 448
FblI GTMKAC 1 cut(s) 1017
Fnu4HI GCNGC 3 cut(s) 89, 378, 777
FokI GGATG 2 cut(s) 59, 129
Fsp4HI GCNGC 3 cut(s) 89, 378, 777
FspBI CTAG 6 cut(s) 276, 360, 401, 737, 851, 1005
GlaI GCGC 1 cut(s) 338
GluI GCNGC 3 cut(s) 89, 378, 777
GsuI CTGGAG 1 cut(s) 692
HaeIII GGCC 4 cut(s) 175, 809, 823, 833
HgaI GACGC 1 cut(s) 782
HhaI GCGC 1 cut(s) 339
Hin1II CATG 8 cut(s) 31, 169, 299, 373, 502, 592, 885, 894
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 2 cut(s) 677, 989
Hpy166II GTNNAC 1 cut(s) 1018
Hpy188I TCNGA 1 cut(s) 795
Hpy188III TCNNGA 3 cut(s) 210, 254, 467
Hpy8I GTNNAC 1 cut(s) 1018
HpyAV CCTTC 3 cut(s) 430, 736, 853
HpyCH4III ACNGT 3 cut(s) 384, 761, 956
HpyCH4V TGCA 4 cut(s) 91, 827, 876, 885
HpyF10VI GCNNNNNNNGC 9 cut(s) 276, 430, 641, 882, 887, 891, 896, 931, 984
HpyF3I CTNAG 4 cut(s) 150, 271, 420, 560
Hsp92II CATG 8 cut(s) 31, 169, 299, 373, 502, 592, 885, 894
HspAI GCGC 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 5 cut(s) 127, 265, 427, 448, 790
LguI GCTCTTC 1 cut(s) 692
LmnI GCTCC 1 cut(s) 862
Lsp1109I GCAGC 3 cut(s) 75, 389, 763
LweI GCATC 1 cut(s) 912
MaeI CTAG 6 cut(s) 276, 360, 401, 737, 851, 1005
MaeIII GTNAC 1 cut(s) 349
MalI GATC 5 cut(s) 129, 267, 429, 450, 792
MboI GATC 5 cut(s) 127, 265, 427, 448, 790
MboII GAAGA 3 cut(s) 212, 452, 709
MlsI TGGCCA 1 cut(s) 809
MluNI TGGCCA 1 cut(s) 809
MlyI GAGTC 2 cut(s) 686, 998
MmeI TCCRAC 2 cut(s) 92, 818
MnlI CCTC 5 cut(s) 463, 716, 739, 790, 898
Mox20I TGGCCA 1 cut(s) 809
MscI TGGCCA 1 cut(s) 809
MseI TTAA 1 cut(s) 311
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 809
MspA1I CMGCKG 2 cut(s) 88, 113
MspR9I CCNGG 2 cut(s) 190, 806
Mva1269I GAATGC 1 cut(s) 279
MvaI CCWGG 2 cut(s) 190, 806
MwoI GCNNNNNNNGC 9 cut(s) 276, 430, 641, 882, 887, 891, 896, 931, 984
NdeI CATATG 1 cut(s) 513
NdeII GATC 5 cut(s) 127, 265, 427, 448, 790
NheI GCTAGC 1 cut(s) 275
NlaIII CATG 8 cut(s) 31, 169, 299, 373, 502, 592, 885, 894
NlaIV GGNNCC 1 cut(s) 935
NmeAIII GCCGAG 1 cut(s) 920
NspI RCATGY 3 cut(s) 373, 885, 894
PaeI GCATGC 3 cut(s) 373, 885, 894
PciSI GCTCTTC 1 cut(s) 692
PctI GAATGC 1 cut(s) 279
PkrI GCNGC 3 cut(s) 90, 379, 778
PleI GAGTC 2 cut(s) 685, 997
PpsI GAGTC 2 cut(s) 685, 997
Psp6I CCWGG 2 cut(s) 188, 804
PspGI CCWGG 2 cut(s) 188, 804
PspN4I GGNNCC 1 cut(s) 935
PspPI GGNCC 1 cut(s) 821
PstI CTGCAG 1 cut(s) 93
PvuII CAGCTG 2 cut(s) 88, 113
RsaI GTAC 3 cut(s) 108, 455, 671
RsaNI GTAC 3 cut(s) 107, 454, 670
RseI CAYNNNNRTG 1 cut(s) 164
SapI GCTCTTC 1 cut(s) 692
SaqAI TTAA 1 cut(s) 311
SatI GCNGC 3 cut(s) 89, 378, 777
Sau3AI GATC 5 cut(s) 127, 265, 427, 448, 790
Sau96I GGNCC 1 cut(s) 821
SchI GAGTC 2 cut(s) 686, 998
ScrFI CCNGG 2 cut(s) 190, 806
SfaNI GCATC 1 cut(s) 912
SfcI CTRYAG 2 cut(s) 89, 534
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 208
SmoI CTYRAG 1 cut(s) 208
SphI GCATGC 3 cut(s) 373, 885, 894
SsiI CCGC 2 cut(s) 531, 595
SspI AATATT 1 cut(s) 412
SspMI CTAG 6 cut(s) 276, 360, 401, 737, 851, 1005
StyD4I CCNGG 2 cut(s) 188, 804
StyI CCWWGG 3 cut(s) 400, 736, 850
TaaI ACNGT 3 cut(s) 384, 761, 956
TaqI TCGA 3 cut(s) 302, 615, 639
TatI WGTACW 2 cut(s) 453, 669
Tru1I TTAA 1 cut(s) 311
Tru9I TTAA 1 cut(s) 311
TscAI CASTG 2 cut(s) 766, 976
TseI GCWGC 3 cut(s) 88, 377, 776
TspDTI ATGAA 6 cut(s) 63, 273, 515, 605, 955, 1010
TspRI CASTG 2 cut(s) 766, 976
XagI CCTNNNNNAGG 1 cut(s) 740
XapI RAATTY 3 cut(s) 228, 443, 462
XceI RCATGY 3 cut(s) 373, 885, 894
XmaJI CCTAGG 3 cut(s) 400, 736, 850
XmiI GTMKAC 1 cut(s) 1017
XspI CTAG 6 cut(s) 276, 360, 401, 737, 851, 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.