Rroxscaffold_2G00131100

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000662
Physical Location & Seq
Forward (+)
67642098 .. 67645736
3639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00131100.1

Sequence Viewer

Length: 3243 bp
ATGGCTTCCTCAGCGGCTCCTATTTCTGACAGCCTCAAGAAGATCTGGGATCAGTGGAATCTCCGGAGCTTCATCATGTTAAGTCTCACATTGCAGACCTTCTTAATTCTCTTTGCACCCTTGAGAAAGCGATCATCGAGCAAATGGGTAATCGTGCCCATCTGGTTGGCTTACTTGCTCGCTGACTGGGCTGCTAACTTCGCTATCGGACTAATTGCAAGCAGCCAAACGAACATTGATCAAGGTTCGCCAGAGAATGGTAATCTCTTGGCATTCTGGGCTCCCTTTCTTCTTTTGCACCTTGGTGGTCCAGACACCATAATTGCAATTGCTATGGAAGACAATGCGTTGTGGCCAAGGCACTTGCTTGGTCTCATATTCCAGGTTGTAGCTGCAATGTACGTCTTCGGTATGCAGTTCTCTAACCAAAACAAGCTACTGTGGCCAACACTCTTCCTGTTTGTACCAGGACTTGTCAAGTACGGCGAGCGAACACGTGCTTTATATCTTGCCAGCTTGGAAAGTCTCAAGGAAACCATACTTGCAGTACCGGAACCTGGACCGGATTATGCAAAGTACATGGATGAGTACTCCTCAAGAAAAGCTGCCCATCTTCCAATTAAAATAGAAATCAAAGAAGGTCGTAGCTTAGATTCGAAGAATGTGACGTATCGGCTGGGAGAAGGAGACGTCATTGATAATATTACACTAGTGGAGGGTGCTCATCACTTGTTTCAAATCTCCATGGGTCTTATTATAGATCTTGTGTTTGACTATCATCAGCGCAAAGAGAGCCGTGCCTATTTCATCAAATTAGAATCAGGAGATGCTTTTACATCAATTGCTACTGAGCTCAATTTTATGTATGAAGCTCTCTACACCAAAGTTTATGTGGTACATTCTCGACGGGGATACTTGTTGAGAGCTACATCCTTCATCGCCGTTTGCATAGCCTTTGTTTTCTTCTATAGATTAGAGAAACATGGCTTTCACAGCTTTGATGTTGGAATTACTTATTCCTTGCTTTTTGGTGCCATAGCTCTTGATTCAATAGCTCTTTCGATGCTGATATTATCTGATTGGACGATCGCTGTTGTATACAAGTACTGTAAAAAGTGTTGTGTAGTCACTGATCTCATGATGAAGTACCTGGAGTTTAAGAGATCATGGCGACCAACTACTCCACCAAACTGTCTGGAGCGGACCAGAAAGATTTTATTTCGAAGATGGTGTGAATCTGTATCCGGTTTCAGCTTCATAGATTATTCTCTCCGAGAACGTGGAAGATCAGTAGTCCGACCTGTGTACCGTTTTGGCATTTTTGACCTTAACCGTACAGTCATCAGATTTTTGGGCTACCTTTACCATAGAATCATCATATTCCTGGACCACCTTTACCTTAGAATCATTAAAGTCTTTGGCCTCAAGTATCTTCGAGATGAGATGAAGTATAGATCCAGCAAGAGACTGGAAGAGAATTTGTGGGAGTTCATTTTTGATGAGTTGAAAAGTAAATCCTCAGCAGTAGTTGATGATCCCAAAATTGGTAGAGAAATATGTTCGTCCCAAGGGGATTGGGTTCTCCTAAAAAAGTACGAGGAGCATCCGGAGTACAAGAACTTGCTAGCATGCTTTGTTGATGTTACTTATGACCGACGCGTTCTGATGTGGCATATTGCTACTGAGATGTGCTATTACAGAGTTGACACAGGCAATGGCGGTGAGCATGGTGAATATAGTAAGACGTTGTCTGATTATTTGCTATATCTCTTAATTATGCAACCTGCTTTGATGTCCTCTGTAGCGGGAATTGGACAAAAGAGATTTAGAGATACGGTTGCAGAAGCCAAAAAATTCTTCAAAAAAGGGCTATTGGGATCGGATGTGAAACTAGGCGAGGCTTGTTACAAACTCCTTGATGTAGATACAGGAGTTAAACCTGTCAATGTCAAAGGGAATAGAAGCAAATCTGTTTTGTTTGATGCATGTATTCTGGCCAAGCAGCTGATGAAATTCGAAGACTCGAAATGGGAATTAATAAGCTCGGTGTGGGTGGAATTGCTATCATACGCTGCCAGTCATTGCAGAGCACAAAGCCACGCGCAATTGCTGACTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTACTGATGGCTCGCTTTGGCATTGATGAGGACATCCAAGTACTGGAGGTCCAAGCAAGAGTAAAACTGATCATGGAAAAGCAACCTGATGTATTCCAGATCACTGATCTCATGAAGAAGTACCTGGAGTTTAAGAGATCATGGCGACCAACTACTACACCAAAGTGTCTGGAGTGGACCAGAAGGATTTTATTTCGAAGATGGTGTGAATCTGTCTCGGGTTTCAGCTTCATAGATTATTCTCTCCGAGAATGTGGAAGAACAAGATCAGTAGTCCAACCTGCAATTTTGGACCAAATTGGCATTTACCGAAGGATCATCAGATTTTTGGGTTACCTTTACTTTAGAATCATCAAATTCCCTGGCTACCTTTACCTTAGAATCATCATATTCCTGGGCCTCAAGTATCTTCGAGATGAGAGGAAGTATAGATCCAGCAAGAGACTGGATGAAAATTTGTGGAATTTCATTTTTGATGAGTTGAAAATTAAATCCTTGGAATCAGGAGCAGATGATGTTGATCCCGAAATTGCTAGAAAAATGTGTTCGTCCCAAGGGGATTGGGTTCTCCAAACAAAGTATCAGGATCATCCGGAGTACAAGAACTTACTATTGTGCTTTGTTGATATTAGTCATTTTCCTTATGACCGACGGGTTCTGCTGTGGCATATTGCTACTGAGATGTGTTATTACAGAGATGATACAGGCAATGGTGGTGATAATCGCGAGAGGAAACTACGCGAGGCTTGTTGCAAACTCCTTGATGTAGATACAGGAGTTAAACCAGTCGATGTCAAAGGGAATAGAAGTAAATCTGTTTTGTTTGATGCATGTATTTTGGCCAGGCAGCTGATGAAATTCGAAGACAAGAAATGGGAATTAATAAGCTCGGTGTGGGTGGAGTTGCTATCATATGCTGCGAGTCATTGCAGAGCACATAGCCATGCGCAATTGCTGACTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTACTGATGGCTCGCTTTGGCATTGAGGAGGACATCCAAGTACTGGAGGTCCAAGCAAGAGTAAAATTGATCATGGAAAAGCAACCTGATGTATTCCAGAGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

1080

Amino Acids

125.42

Weight (kDa)

8.96

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 422 5.8e-90 Domain of unknown function (DUF4220)
DUF594 PF04578 673 - 723 1.2e-21 Protein of unknown function, DUF594
DUF594 PF04578 1003 - 1053 1.9e-21 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 693
Acc16I TGCGCA 1 cut(s) 3093
Acc36I ACCTGC 2 cut(s) 1792, 2434
AccB1I GGYRCC 1 cut(s) 1031
AccB7I CCANNNNNTGG 3 cut(s) 257, 2188, 3178
AccBSI CCGCTC 1 cut(s) 1201
AccI GTMKAC 1 cut(s) 1098
AccII CGCG 4 cut(s) 1659, 2102, 2871, 2886
AccIII TCCGGA 3 cut(s) 63, 1606, 2737
AciI CCGC 4 cut(s) 14, 1201, 1719, 1805
AclWI GGATC 8 cut(s) 57, 1449, 1529, 1885, 2468, 2571, 2660, 2739
AcoI YGGCCR 4 cut(s) 353, 443, 1995, 2985
AcsI RAATTY 7 cut(s) 1477, 1853, 2012, 2501, 2599, 2608, 3002
AcvI CACGTG 1 cut(s) 497
AcyI GRCGYC 1 cut(s) 690
AfiI CCNNNNNNNGG 5 cut(s) 257, 557, 1544, 2188, 3178
AflIII ACRYGT 2 cut(s) 494, 1657
AgsI TTSAA 5 cut(s) 737, 1050, 1507, 1861, 2629
AhlI ACTAGT 1 cut(s) 709
AjnI CCWGG 9 cut(s) 381, 466, 556, 1149, 1383, 2268, 2506, 2538, 2987
AjuI GAANNNNNNNTTGG 2 cut(s) 1841, 1873
AleI CACNNNNGTG 2 cut(s) 303, 2308
Alw21I GWGCWC 4 cut(s) 724, 855, 2092, 3082
Alw26I GTCTC 7 cut(s) 89, 377, 530, 681, 1459, 2365, 2581
AlwI GGATC 8 cut(s) 57, 1449, 1529, 1885, 2468, 2571, 2660, 2739
Ama87I CYCGRG 1 cut(s) 2362
Aor13HI TCCGGA 3 cut(s) 63, 1606, 2737
AoxI GGCC 6 cut(s) 353, 443, 1420, 1995, 2542, 2985
ApeKI GCWGC 8 cut(s) 191, 222, 392, 605, 2002, 2072, 2992, 3062
ApoI RAATTY 7 cut(s) 1477, 1853, 2012, 2501, 2599, 2608, 3002
AseI ATTAAT 2 cut(s) 2036, 3026
AspLEI GCGC 3 cut(s) 786, 2104, 3094
AspS9I GGNCC 9 cut(s) 308, 560, 1203, 1387, 2194, 2322, 2437, 2542, 3184
AsuHPI GGTGA 3 cut(s) 1733, 1742, 2873
AsuII TTCGAA 5 cut(s) 656, 1222, 2016, 2341, 3006
AsuNHI GCTAGC 1 cut(s) 1624
AvaI CYCGRG 1 cut(s) 2362
AvaII GGWCC 8 cut(s) 308, 560, 1203, 1387, 2194, 2322, 2437, 3184
BaeGI GKGCMC 1 cut(s) 159
BaeI ACNNNNGTAYC 6 cut(s) 1289, 1322, 1917, 1950, 2907, 2940
BalI TGGCCA 4 cut(s) 355, 445, 1997, 2987
BanI GGYRCC 1 cut(s) 1031
BanII GRGCYC 2 cut(s) 283, 855
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 4 cut(s) 345, 397, 2025, 3015
Bbv12I GWGCWC 4 cut(s) 724, 855, 2092, 3082
BbvCI CCTCAGC 2 cut(s) 10, 1519
BbvI GCAGC 8 cut(s) 178, 234, 379, 592, 2014, 2059, 3004, 3049
BccI CCATC 6 cut(s) 167, 618, 1221, 2146, 2340, 3136
BceAI ACGGC 3 cut(s) 499, 780, 926
BciT130I CCWGG 9 cut(s) 383, 468, 558, 1151, 1385, 2270, 2508, 2540, 2989
BciVI GTATCC 2 cut(s) 905, 1252
BclI TGATCA 3 cut(s) 238, 2214, 3204
BcoDI GTCTC 7 cut(s) 89, 377, 530, 681, 1459, 2365, 2581
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 4 cut(s) 710, 1625, 1892, 2679
BfmI CTRYAG 2 cut(s) 967, 1800
BfuAI ACCTGC 2 cut(s) 1792, 2434
BfuI GTATCC 2 cut(s) 905, 1252
BglII AGATCT 2 cut(s) 42, 760
BisI GCNGC 9 cut(s) 15, 192, 223, 393, 606, 2003, 2073, 2993, 3063
BlsI GCNGC 9 cut(s) 16, 193, 224, 394, 607, 2004, 2074, 2994, 3064
BmcAI AGTACT 4 cut(s) 590, 1106, 2187, 3177
Bme1390I CCNGG 9 cut(s) 383, 468, 558, 1151, 1385, 2270, 2508, 2540, 2989
Bme18I GGWCC 8 cut(s) 308, 560, 1203, 1387, 2194, 2322, 2437, 3184
BmeT110I CYCGRG 1 cut(s) 2362
BmgT120I GGNCC 9 cut(s) 308, 560, 1203, 1387, 2194, 2322, 2437, 2542, 3184
BmiI GGNNCC 4 cut(s) 18, 282, 555, 1033
BmrFI CCNGG 9 cut(s) 383, 468, 558, 1151, 1385, 2270, 2508, 2540, 2989
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 5 cut(s) 817, 1053, 1612, 1972, 2962
BmtI GCTAGC 1 cut(s) 1628
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 4 cut(s) 345, 397, 2025, 3015
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 6 cut(s) 1172, 1217, 2210, 2291, 2336, 3200
Bpu10I CCTNAGC 2 cut(s) 10, 1519
Bpu14I TTCGAA 5 cut(s) 656, 1222, 2016, 2341, 3006
BpuEI CTTGAG 6 cut(s) 20, 142, 512, 580, 1409, 2531
BsaAI YACGTR 1 cut(s) 497
BsaBI GATNNNNATC 1 cut(s) 2664
BsaHI GRCGYC 1 cut(s) 690
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 8 cut(s) 301, 356, 744, 1567, 2506, 2539, 2640, 2698
BsaWI WCCGGW 6 cut(s) 63, 550, 562, 1244, 1606, 2737
BsaXI ACNNNNNCTCC 4 cut(s) 2115, 2145, 3105, 3135
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 557, 1544, 2188, 3178
Bse1I ACTGG 7 cut(s) 191, 1473, 2076, 2193, 2595, 2930, 3183
Bse3DI GCAATG 6 cut(s) 89, 402, 1720, 2080, 2860, 3070
Bse8I GATNNNNATC 1 cut(s) 2664
BseAI TCCGGA 3 cut(s) 63, 1606, 2737
BseBI CCWGG 9 cut(s) 383, 468, 558, 1151, 1385, 2270, 2508, 2540, 2989
BseDI CCNNGG 8 cut(s) 301, 356, 744, 1567, 2506, 2539, 2640, 2698
BseGI GGATG 8 cut(s) 589, 929, 1603, 1888, 2178, 2599, 2734, 3168
BseJI GATNNNNATC 1 cut(s) 2664
BseLI CCNNNNNNNGG 5 cut(s) 257, 557, 1544, 2188, 3178
BseMI GCAATG 6 cut(s) 89, 402, 1720, 2080, 2860, 3070
BseMII CTCAG 5 cut(s) 24, 840, 1533, 1674, 2814
BseNI ACTGG 7 cut(s) 191, 1473, 2076, 2193, 2595, 2930, 3183
BseRI GAGGAG 3 cut(s) 583, 1613, 3176
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 8 cut(s) 178, 234, 379, 592, 2014, 2059, 3004, 3049
BseYI CCCAGC 1 cut(s) 676
Bsh1236I CGCG 4 cut(s) 1659, 2102, 2871, 2886
Bsh1285I CGRYCG 1 cut(s) 1089
BshFI GGCC 6 cut(s) 355, 445, 1422, 1997, 2544, 2987
BshNI GGYRCC 1 cut(s) 1031
BsiEI CGRYCG 1 cut(s) 1089
BsiHKAI GWGCWC 4 cut(s) 724, 855, 2092, 3082
BsiHKCI CYCGRG 1 cut(s) 2362
BsiSI CCGG 6 cut(s) 64, 551, 563, 1245, 1607, 2738
BslFI GGGAC 2 cut(s) 1549, 2680
BslI CCNNNNNNNGG 5 cut(s) 257, 557, 1544, 2188, 3178
BsmAI GTCTC 7 cut(s) 89, 377, 530, 681, 1459, 2365, 2581
BsmBI CGTCTC 1 cut(s) 681
BsmFI GGGAC 2 cut(s) 1549, 2680
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 6 cut(s) 355, 445, 1422, 1997, 2544, 2987
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 1 cut(s) 2362
Bsp119I TTCGAA 5 cut(s) 656, 1222, 2016, 2341, 3006
Bsp1286I GDGCHC 6 cut(s) 159, 283, 724, 855, 2092, 3082
Bsp13I TCCGGA 3 cut(s) 63, 1606, 2737
Bsp19I CCATGG 1 cut(s) 744
Bsp68I TCGCGA 1 cut(s) 2871
BspACI CCGC 4 cut(s) 14, 1201, 1719, 1805
BspANI GGCC 6 cut(s) 355, 445, 1422, 1997, 2544, 2987
BspCNI CTCAG 5 cut(s) 23, 841, 1532, 1675, 2815
BspEI TCCGGA 3 cut(s) 63, 1606, 2737
BspFNI CGCG 4 cut(s) 1659, 2102, 2871, 2886
BspHI TCATGA 2 cut(s) 1137, 2256
BspLI GGNNCC 4 cut(s) 18, 282, 555, 1033
BspMI ACCTGC 2 cut(s) 1792, 2434
BspOI GCTAGC 1 cut(s) 1628
BspPI GGATC 8 cut(s) 57, 1449, 1529, 1885, 2468, 2571, 2660, 2739
BspT104I TTCGAA 5 cut(s) 656, 1222, 2016, 2341, 3006
BspT107I GGYRCC 1 cut(s) 1031
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 1201
BsrDI GCAATG 6 cut(s) 89, 402, 1720, 2080, 2860, 3070
BsrI ACTGG 7 cut(s) 191, 1473, 2076, 2193, 2595, 2930, 3183
BssECI CCNNGG 8 cut(s) 301, 356, 744, 1567, 2506, 2539, 2640, 2698
BssNAI GTATAC 1 cut(s) 1099
BssNI GRCGYC 1 cut(s) 690
BssT1I CCWWGG 6 cut(s) 301, 356, 744, 1567, 2640, 2698
Bst1107I GTATAC 1 cut(s) 1099
Bst2UI CCWGG 9 cut(s) 383, 468, 558, 1151, 1385, 2270, 2508, 2540, 2989
Bst4CI ACNGT 7 cut(s) 441, 1109, 1193, 1310, 1334, 1339, 1837
Bst6I CTCTTC 2 cut(s) 458, 1467
BstACI GRCGYC 1 cut(s) 690
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 5 cut(s) 656, 1222, 2016, 2341, 3006
BstC8I GCNNGC 8 cut(s) 180, 220, 488, 514, 1626, 1630, 2158, 3148
BstDEI CTNAG 8 cut(s) 10, 649, 849, 1400, 1519, 1683, 2522, 2823
BstDSI CCRYGG 1 cut(s) 744
BstEII GGTNACC 1 cut(s) 2477
BstF5I GGATG 8 cut(s) 589, 929, 1603, 1888, 2178, 2599, 2734, 3168
BstFNI CGCG 4 cut(s) 1659, 2102, 2871, 2886
BstHHI GCGC 3 cut(s) 786, 2104, 3094
BstMAI GTCTC 7 cut(s) 89, 377, 530, 681, 1459, 2365, 2581
BstMCI CGRYCG 1 cut(s) 1089
BstMWI GCNNNNNNNGC 7 cut(s) 11, 188, 200, 278, 442, 792, 993
BstNI CCWGG 9 cut(s) 383, 468, 558, 1151, 1385, 2270, 2508, 2540, 2989
BstNSI RCATGY 3 cut(s) 1632, 1989, 2979
BstPI GGTNACC 1 cut(s) 2477
BstSCI CCNGG 9 cut(s) 381, 466, 556, 1149, 1383, 2268, 2506, 2538, 2987
BstSFI CTRYAG 2 cut(s) 967, 1800
BstSLI GKGCMC 1 cut(s) 159
BstUI CGCG 4 cut(s) 1659, 2102, 2871, 2886
BstV1I GCAGC 8 cut(s) 178, 234, 379, 592, 2014, 2059, 3004, 3049
BstV2I GAAGAC 4 cut(s) 345, 397, 2025, 3015
BstX2I RGATCY 4 cut(s) 42, 760, 1454, 2576
BstXI CCANNNNNNTGG 1 cut(s) 166
BstYI RGATCY 4 cut(s) 42, 760, 1454, 2576
BstZ17I GTATAC 1 cut(s) 1099
BsuI GTATCC 2 cut(s) 905, 1252
BsuRI GGCC 6 cut(s) 355, 445, 1422, 1997, 2544, 2987
BtgI CCRYGG 1 cut(s) 744
BtgZI GCGATG 1 cut(s) 922
BtsCI GGATG 8 cut(s) 589, 929, 1603, 1888, 2178, 2599, 2734, 3168
BtsIMutI CAGTG 3 cut(s) 59, 1128, 2247
BtuMI TCGCGA 1 cut(s) 2871
BveI ACCTGC 2 cut(s) 1792, 2434
Cac8I GCNNGC 8 cut(s) 180, 220, 488, 514, 1626, 1630, 2158, 3148
CciI TCATGA 2 cut(s) 1137, 2256
CfoI GCGC 3 cut(s) 786, 2104, 3094
Cfr13I GGNCC 9 cut(s) 308, 560, 1203, 1387, 2194, 2322, 2437, 2542, 3184
CseI GACGC 1 cut(s) 1665
DdeI CTNAG 8 cut(s) 10, 649, 849, 1400, 1519, 1683, 2522, 2823
EaeI YGGCCR 4 cut(s) 353, 443, 1995, 2985
Eam1104I CTCTTC 2 cut(s) 458, 1467
EarI CTCTTC 2 cut(s) 458, 1467
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 6 cut(s) 301, 356, 744, 1567, 2640, 2698
Eco24I GRGCYC 2 cut(s) 283, 855
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 8 cut(s) 308, 560, 1203, 1387, 2194, 2322, 2437, 3184
Eco53kI GAGCTC 1 cut(s) 853
Eco72I CACGTG 1 cut(s) 497
Eco88I CYCGRG 1 cut(s) 2362
Eco91I GGTNACC 1 cut(s) 2477
EcoICRI GAGCTC 1 cut(s) 853
EcoO65I GGTNACC 1 cut(s) 2477
EcoRII CCWGG 9 cut(s) 381, 466, 556, 1149, 1383, 2268, 2506, 2538, 2987
EcoT14I CCWWGG 6 cut(s) 301, 356, 744, 1567, 2640, 2698
EcoT22I ATGCAT 2 cut(s) 1987, 2977
EcoT38I GRGCYC 2 cut(s) 283, 855
ErhI CCWWGG 6 cut(s) 301, 356, 744, 1567, 2640, 2698
Esp3I CGTCTC 1 cut(s) 681
FaqI GGGAC 2 cut(s) 1549, 2680
FauI CCCGC 1 cut(s) 1798
FauNDI CATATG 1 cut(s) 3058
FbaI TGATCA 3 cut(s) 238, 2214, 3204
FblI GTMKAC 1 cut(s) 1098
Fnu4HI GCNGC 9 cut(s) 15, 192, 223, 393, 606, 2003, 2073, 2993, 3063
FokI GGATG 8 cut(s) 596, 916, 1590, 1895, 2165, 2606, 2721, 3155
FriOI GRGCYC 2 cut(s) 283, 855
Fsp4HI GCNGC 9 cut(s) 15, 192, 223, 393, 606, 2003, 2073, 2993, 3063
FspBI CTAG 4 cut(s) 710, 1625, 1892, 2679
FspI TGCGCA 1 cut(s) 3093
GlaI GCGC 3 cut(s) 785, 2103, 3093
GluI GCNGC 9 cut(s) 15, 192, 223, 393, 606, 2003, 2073, 2993, 3063
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 6 cut(s) 1172, 1217, 2210, 2291, 2336, 3200
HaeIII GGCC 6 cut(s) 355, 445, 1422, 1997, 2544, 2987
HapII CCGG 6 cut(s) 64, 551, 563, 1245, 1607, 2738
HgaI GACGC 1 cut(s) 1665
HhaI GCGC 3 cut(s) 786, 2104, 3094
Hin1I GRCGYC 1 cut(s) 690
Hin6I GCGC 3 cut(s) 784, 2102, 3092
HinP1I GCGC 3 cut(s) 784, 2102, 3092
HincII GTYRAC 1 cut(s) 1705
HindII GTYRAC 1 cut(s) 1705
HpaII CCGG 6 cut(s) 64, 551, 563, 1245, 1607, 2738
HphI GGTGA 3 cut(s) 1733, 1742, 2873
Hpy166II GTNNAC 4 cut(s) 1099, 1306, 1705, 2322
Hpy8I GTNNAC 4 cut(s) 1099, 1306, 1705, 2322
Hpy99I CGWCG 3 cut(s) 909, 1659, 2799
HpyAV CCTTC 6 cut(s) 109, 632, 677, 943, 2322, 2451
HpyCH4III ACNGT 7 cut(s) 441, 1109, 1193, 1310, 1334, 1339, 1837
HpyCH4IV ACGT 6 cut(s) 402, 496, 668, 690, 1279, 1745
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 188, 200, 278, 442, 792, 993
HpyF3I CTNAG 8 cut(s) 10, 649, 849, 1400, 1519, 1683, 2522, 2823
HpySE526I ACGT 6 cut(s) 402, 496, 668, 690, 1279, 1745
Hsp92I GRCGYC 1 cut(s) 690
HspAI GCGC 3 cut(s) 784, 2102, 3092
Kpn2I TCCGGA 3 cut(s) 63, 1606, 2737
Ksp22I TGATCA 3 cut(s) 238, 2214, 3204
LmnI GCTCC 6 cut(s) 22, 66, 286, 1198, 1600, 2651
Lsp1109I GCAGC 8 cut(s) 178, 234, 379, 592, 2014, 2059, 3004, 3049
LweI GCATC 5 cut(s) 817, 1053, 1612, 1972, 2962
MaeI CTAG 4 cut(s) 710, 1625, 1892, 2679
MaeII ACGT 6 cut(s) 402, 496, 668, 690, 1279, 1745
MaeIII GTNAC 9 cut(s) 664, 1126, 1642, 1904, 2131, 2145, 2477, 3121, 3135
MbiI CCGCTC 1 cut(s) 1201
MfeI CAATTG 4 cut(s) 327, 840, 2105, 3095
MflI RGATCY 4 cut(s) 42, 760, 1454, 2576
MhlI GDGCHC 6 cut(s) 159, 283, 724, 855, 2092, 3082
MlsI TGGCCA 4 cut(s) 355, 445, 1997, 2987
MluI ACGCGT 1 cut(s) 1657
MluNI TGGCCA 4 cut(s) 355, 445, 1997, 2987
MlyI GAGTC 2 cut(s) 2015, 3076
MmeI TCCRAC 3 cut(s) 985, 1321, 2446
Mox20I TGGCCA 4 cut(s) 355, 445, 1997, 2987
Mph1103I ATGCAT 2 cut(s) 1987, 2977
MroI TCCGGA 3 cut(s) 63, 1606, 2737
MscI TGGCCA 4 cut(s) 355, 445, 1997, 2987
MslI CAYNNNNRTG 3 cut(s) 303, 2308, 3087
Msp20I TGGCCA 4 cut(s) 355, 445, 1997, 2987
MspA1I CMGCKG 3 cut(s) 14, 2005, 2995
MspI CCGG 6 cut(s) 64, 551, 563, 1245, 1607, 2738
MspR9I CCNGG 9 cut(s) 383, 468, 558, 1151, 1385, 2270, 2508, 2540, 2989
MunI CAATTG 4 cut(s) 327, 840, 2105, 3095
Mva1269I GAATGC 1 cut(s) 272
MvaI CCWGG 9 cut(s) 383, 468, 558, 1151, 1385, 2270, 2508, 2540, 2989
MvnI CGCG 4 cut(s) 1659, 2102, 2871, 2886
MwoI GCNNNNNNNGC 7 cut(s) 11, 188, 200, 278, 442, 792, 993
NcoI CCATGG 1 cut(s) 744
NdeI CATATG 1 cut(s) 3058
NheI GCTAGC 1 cut(s) 1624
NlaIV GGNNCC 4 cut(s) 18, 282, 555, 1033
NmuCI GTSAC 4 cut(s) 664, 1126, 2131, 3121
NruI TCGCGA 1 cut(s) 2871
NsbI TGCGCA 1 cut(s) 3093
NsiI ATGCAT 2 cut(s) 1987, 2977
NspI RCATGY 3 cut(s) 1632, 1989, 2979
NspV TTCGAA 5 cut(s) 656, 1222, 2016, 2341, 3006
OliI CACNNNNGTG 2 cut(s) 303, 2308
PaeI GCATGC 1 cut(s) 1632
PagI TCATGA 2 cut(s) 1137, 2256
PctI GAATGC 1 cut(s) 272
PflFI GACNNNGTC 1 cut(s) 1747
PflMI CCANNNNNTGG 3 cut(s) 257, 2188, 3178
PfoI TCCNGGA 1 cut(s) 1383
PkrI GCNGC 9 cut(s) 16, 193, 224, 394, 607, 2004, 2074, 2994, 3064
Ple19I CGATCG 1 cut(s) 1089
PleI GAGTC 2 cut(s) 2015, 3075
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 2 cut(s) 2015, 3075
Ppu21I YACGTR 1 cut(s) 497
PshBI ATTAAT 2 cut(s) 2036, 3026
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 9 cut(s) 381, 466, 556, 1149, 1383, 2268, 2506, 2538, 2987
PspCI CACGTG 1 cut(s) 497
PspEI GGTNACC 1 cut(s) 2477
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 9 cut(s) 381, 466, 556, 1149, 1383, 2268, 2506, 2538, 2987
PspN4I GGNNCC 4 cut(s) 18, 282, 555, 1033
PspPI GGNCC 9 cut(s) 308, 560, 1203, 1387, 2194, 2322, 2437, 2542, 3184
PsuI RGATCY 4 cut(s) 42, 760, 1454, 2576
PsyI GACNNNGTC 1 cut(s) 1747
PvuI CGATCG 1 cut(s) 1089
PvuII CAGCTG 2 cut(s) 2005, 2995
RruI TCGCGA 1 cut(s) 2871
RseI CAYNNNNRTG 3 cut(s) 303, 2308, 3087
SacI GAGCTC 1 cut(s) 855
SatI GCNGC 9 cut(s) 15, 192, 223, 393, 606, 2003, 2073, 2993, 3063
Sau96I GGNCC 9 cut(s) 308, 560, 1203, 1387, 2194, 2322, 2437, 2542, 3184
ScaI AGTACT 4 cut(s) 590, 1106, 2187, 3177
SchI GAGTC 2 cut(s) 2015, 3076
ScrFI CCNGG 9 cut(s) 383, 468, 558, 1151, 1385, 2270, 2508, 2540, 2989
SduI GDGCHC 6 cut(s) 159, 283, 724, 855, 2092, 3082
SfaNI GCATC 5 cut(s) 817, 1053, 1612, 1972, 2962
SfcI CTRYAG 2 cut(s) 967, 1800
SfuI TTCGAA 5 cut(s) 656, 1222, 2016, 2341, 3006
SinI GGWCC 8 cut(s) 308, 560, 1203, 1387, 2194, 2322, 2437, 3184
SmiMI CAYNNNNRTG 3 cut(s) 303, 2308, 3087
SmlI CTYRAG 6 cut(s) 35, 121, 527, 595, 1424, 2546
SmoI CTYRAG 6 cut(s) 35, 121, 527, 595, 1424, 2546
SpeI ACTAGT 1 cut(s) 709
SphI GCATGC 1 cut(s) 1632
SsiI CCGC 4 cut(s) 14, 1201, 1719, 1805
SspI AATATT 1 cut(s) 703
SspMI CTAG 4 cut(s) 710, 1625, 1892, 2679
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 9 cut(s) 381, 466, 556, 1149, 1383, 2268, 2506, 2538, 2987
StyI CCWWGG 6 cut(s) 301, 356, 744, 1567, 2640, 2698
TaaI ACNGT 7 cut(s) 441, 1109, 1193, 1310, 1334, 1339, 1837
TaiI ACGT 6 cut(s) 405, 499, 671, 693, 1282, 1748
TaqII GACCGA 2 cut(s) 1668, 2808
TatI WGTACW 7 cut(s) 576, 588, 1104, 1611, 2185, 2742, 3175
TauI GCSGC 1 cut(s) 17
TscAI CASTG 3 cut(s) 59, 1135, 2254
TseFI GTSAC 4 cut(s) 664, 1126, 2131, 3121
TseI GCWGC 8 cut(s) 191, 222, 392, 605, 2002, 2072, 2992, 3062
Tsp45I GTSAC 4 cut(s) 664, 1126, 2131, 3121
TspRI CASTG 3 cut(s) 59, 1135, 2254
Tth111I GACNNNGTC 1 cut(s) 1747
Van91I CCANNNNNTGG 3 cut(s) 257, 2188, 3178
VpaK11BI GGWCC 8 cut(s) 308, 560, 1203, 1387, 2194, 2322, 2437, 3184
VspI ATTAAT 2 cut(s) 2036, 3026
XapI RAATTY 7 cut(s) 1477, 1853, 2012, 2501, 2599, 2608, 3002
XceI RCATGY 3 cut(s) 1632, 1989, 2979
XcmI CCANNNNNNNNNTGG 3 cut(s) 889, 1465, 2587
XmiI GTMKAC 1 cut(s) 1098
XspI CTAG 4 cut(s) 710, 1625, 1892, 2679
ZraI GACGTC 1 cut(s) 691
ZrmI AGTACT 4 cut(s) 590, 1106, 2187, 3177
Zsp2I ATGCAT 2 cut(s) 1987, 2977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.