pycom05g16250

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000662
Physical Location & Seq
Forward (+)
19590117 .. 19591654
1538 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g16250.2

Sequence Viewer

Length: 1488 bp
ATGAGCTGGACAGGTCTAATTGTGGATCTCATTTTCAGCTTCCATGAACGCCATGAGAGTCGTGCAGTCTTCCATGAAAGAACACCGAAAGAAGCATTTAAATTAATTGCAGTCGAGCTCAACTTTATGTATGAAGCTCTCTTCACCAAGGCTGCTGTGGTGCATACTAAGCCAGCATACATTCTCCGGGTGGTTTCCTTCAGCGCGATTGTTGTAGCCCTTGTTTTCTTTTATAGATTAGAGAAAGAGGGTTTGCACTCGTTTGATGTTAGAATTACCTATACCTTGCTGTTTGGTGCCATAAGCTTGGATTCAATAGCTACTGTTATGCTTACATTCTCAGATTGGACCATTGCTGCTCTGAATAAGTTTTGGCAAAATTCTGGTCTAGCCAAAACTATTTTTGGAAAGTATCTCGATTTCAAGAGATCGTGGGGGTGGCCTACGGAAAAAACAAGAAATGAGGGCGGCCAAATCCCTAAAACAAAAGCAACGAAATGTTTGGAGTGGATTAGGCGAATCCTATTTCGGAGGTGGTACGAATCTGTCTCTACATTCAACTTGATACATTATTCCCTCAAGGAGAAGCGGAAAAAGTCCCCGAAATGGTATGACTGCTTTGGCATTGGCTACATTAAAGTCATCACCTTATTTGGCCTCAAGGATCTTCATGATAAGTTGACATATAGGACCAAAAAGCCGCTCGCTGAAGAGTTATGGGTTTTCATTTTTGAGCAGTTGATAAGTAAATCCATGCTCGCTGATGATCCTGAAACTGCAAGGAAAATAGGTGCGGCCAGAGGTGATTGGGTTCTCGAGGGTAGCGATTGGGATTGTAATGTGATCACTAAGTTACTATCTTACGTTGTTGATGTGGAATATGACAAGAGCATTTTATTGTGGCACATTGCTACGGAGTTGTGTTTCAACGATGACAAAAGCAAAACTGAAGGTGAAGCTGGGCCTGTTAGTGATGAAACTAAAAAAAGTAAAGAACACAGTAGGACCATATCTGATTATATGTTGTACCTCTTAGTTATGCAGCCTTCTATGATGTCTTCTATAACAGGAATTGGACAAATAAGATTCCGGGACACGTGCGCAGAAGCCAAGAAATTCTTCAGCAGAAGGGATCTAGAATCTGGACAAGTGAAAGAAGCTTGCGAAAAACTCCTGGAGGTAAATACAGATGTTGAGCCTGTTGCTGTCAAAGGAGATAGAAGTAAATCAGTGTTGTTCGATGCTTGTATTCTGGCCAAAGAGTTGAGAAACTTGGAGGACAGGAAATGGGAGCTGATGAGTGAAGTGTGGGTGGAAATTCTGTCTTATACCGCAACTCATTGCCGAGCGAATGACCATGCGCAACTGCTGAGCAAAGGTGGAGAGCTAGTGACTTTTGTTTGGTTATTGATGGCTCATTTTGGCATAGGAGAGCAGTTCCAAATAAATGAGGGCCATGCGAGAGCAAAACTCATCGTGGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

496

Amino Acids

57.05

Weight (kDa)

8.55

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1102, 1362
AccB1I GGYRCC 1 cut(s) 296
AccBSI CCGCTC 1 cut(s) 703
AccII CGCG 1 cut(s) 206
AciI CCGC 5 cut(s) 468, 589, 701, 794, 1332
AclWI GGATC 4 cut(s) 33, 672, 761, 1140
AcoI YGGCCR 3 cut(s) 469, 795, 1254
AcsI RAATTY 3 cut(s) 379, 1115, 1317
AcuI CTGAAG 4 cut(s) 184, 729, 969, 1105
AcvI CACGTG 1 cut(s) 1098
AfaI GTAC 2 cut(s) 539, 1028
AfiI CCNNNNNNNGG 1 cut(s) 606
AflIII ACRYGT 1 cut(s) 1095
AgsI TTSAA 4 cut(s) 315, 424, 559, 928
AjnI CCWGG 1 cut(s) 1173
AjuI GAANNNNNNNTTGG 2 cut(s) 1103, 1135
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 553
AlwI GGATC 4 cut(s) 33, 672, 761, 1140
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 7 cut(s) 440, 469, 655, 795, 962, 1254, 1453
ApeKI GCWGC 3 cut(s) 152, 356, 1042
ApoI RAATTY 3 cut(s) 379, 1115, 1317
ArsI GACNNNNNNTTYG 2 cut(s) 370, 402
AseI ATTAAT 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 1118
AspLEI GCGC 3 cut(s) 206, 1103, 1363
AspS9I GGNCC 5 cut(s) 348, 690, 962, 1005, 1453
AsuC2I CCSGG 2 cut(s) 188, 1091
AsuHPI GGTGA 4 cut(s) 136, 637, 815, 965
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 3 cut(s) 348, 690, 1005
BalI TGGCCA 1 cut(s) 1256
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 1 cut(s) 120
BbrPI CACGTG 1 cut(s) 1098
BbsI GAAGAC 2 cut(s) 61, 1050
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 3 cut(s) 139, 343, 1054
BccI CCATC 1 cut(s) 1405
BciT130I CCWGG 1 cut(s) 1175
BclI TGATCA 1 cut(s) 843
BcnI CCSGG 2 cut(s) 188, 1091
BcoDI GTCTC 1 cut(s) 553
BfaI CTAG 3 cut(s) 389, 1136, 1388
BisI GCNGC 6 cut(s) 153, 357, 469, 701, 795, 1043
BlpI GCTNAGC 1 cut(s) 1370
BlsI GCNGC 6 cut(s) 154, 358, 470, 702, 796, 1044
Bme1390I CCNGG 3 cut(s) 188, 1091, 1175
Bme18I GGWCC 3 cut(s) 348, 690, 1005
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 5 cut(s) 348, 690, 962, 1005, 1453
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 3 cut(s) 188, 1091, 1175
BmsI GCATC 1 cut(s) 1231
BpiI GAAGAC 2 cut(s) 61, 1050
BplI GAGNNNNNCTC 2 cut(s) 1455, 1487
BpmI CTGGAG 1 cut(s) 1196
Bpu1102I GCTNAGC 1 cut(s) 1370
BpuEI CTTGAG 2 cut(s) 563, 644
BpuMI CCSGG 2 cut(s) 188, 1091
BsaAI YACGTR 1 cut(s) 1098
BsaJI CCNNGG 1 cut(s) 147
BsaXI ACNNNNNCTCC 2 cut(s) 1374, 1404
Bsc4I CCNNNNNNNGG 1 cut(s) 606
Bse3DI GCAATG 3 cut(s) 351, 906, 1339
BseBI CCWGG 1 cut(s) 1175
BseDI CCNNGG 1 cut(s) 147
BseLI CCNNNNNNNGG 1 cut(s) 606
BseMI GCAATG 3 cut(s) 351, 906, 1339
BseMII CTCAG 2 cut(s) 354, 1361
BseXI GCAGC 3 cut(s) 139, 343, 1054
BseYI CCCAGC 1 cut(s) 959
BsgI GTGCAG 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 7 cut(s) 442, 471, 657, 797, 964, 1256, 1455
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 2 cut(s) 187, 1090
BslFI GGGAC 2 cut(s) 583, 1106
BslI CCNNNNNNNGG 1 cut(s) 606
BsmAI GTCTC 1 cut(s) 553
BsmFI GGGAC 2 cut(s) 583, 1106
BsnI GGCC 7 cut(s) 442, 471, 657, 797, 964, 1256, 1455
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 6 cut(s) 25, 428, 664, 766, 843, 1132
Bsp1720I GCTNAGC 1 cut(s) 1370
BspACI CCGC 5 cut(s) 468, 589, 701, 794, 1332
BspANI GGCC 7 cut(s) 442, 471, 657, 797, 964, 1256, 1455
BspCNI CTCAG 2 cut(s) 353, 1362
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 1 cut(s) 298
BspPI GGATC 4 cut(s) 33, 672, 761, 1140
BspT107I GGYRCC 1 cut(s) 296
BsrBI CCGCTC 1 cut(s) 703
BsrDI GCAATG 3 cut(s) 351, 906, 1339
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 6 cut(s) 25, 428, 664, 766, 843, 1132
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 1175
Bst4CI ACNGT 2 cut(s) 325, 1001
Bst6I CTCTTC 2 cut(s) 146, 705
BstBAI YACGTR 1 cut(s) 1098
BstC8I GCNNGC 4 cut(s) 174, 705, 759, 1162
BstDEI CTNAG 5 cut(s) 168, 340, 849, 1033, 1370
BstFNI CGCG 1 cut(s) 206
BstHHI GCGC 3 cut(s) 206, 1103, 1363
BstKTI GATC 6 cut(s) 28, 431, 667, 769, 846, 1135
BstMAI GTCTC 1 cut(s) 553
BstMBI GATC 6 cut(s) 25, 428, 664, 766, 843, 1132
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNI CCWGG 1 cut(s) 1175
BstSCI CCNGG 3 cut(s) 186, 1089, 1173
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 3 cut(s) 139, 343, 1054
BstV2I GAAGAC 2 cut(s) 61, 1050
BstX2I RGATCY 3 cut(s) 25, 664, 1132
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 3 cut(s) 25, 664, 1132
BsuRI GGCC 7 cut(s) 442, 471, 657, 797, 964, 1256, 1455
BtsIMutI CAGTG 1 cut(s) 1236
Cac8I GCNNGC 4 cut(s) 174, 705, 759, 1162
CciI TCATGA 1 cut(s) 670
CfoI GCGC 3 cut(s) 206, 1103, 1363
Cfr13I GGNCC 5 cut(s) 348, 690, 962, 1005, 1453
Csp6I GTAC 2 cut(s) 538, 1027
CviAII CATG 7 cut(s) 44, 53, 74, 671, 754, 1358, 1457
CviQI GTAC 2 cut(s) 538, 1027
DdeI CTNAG 5 cut(s) 168, 340, 849, 1033, 1370
DpnI GATC 6 cut(s) 27, 430, 666, 768, 845, 1134
DpnII GATC 6 cut(s) 25, 428, 664, 766, 843, 1132
DraI TTTAAA 1 cut(s) 100
EaeI YGGCCR 3 cut(s) 469, 795, 1254
Eam1104I CTCTTC 2 cut(s) 146, 705
EarI CTCTTC 2 cut(s) 146, 705
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 3 cut(s) 348, 690, 1005
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 4 cut(s) 184, 729, 969, 1105
Eco72I CACGTG 1 cut(s) 1098
Eco88I CYCGRG 1 cut(s) 815
EcoICRI GAGCTC 1 cut(s) 118
EcoRII CCWGG 1 cut(s) 1173
EcoT14I CCWWGG 1 cut(s) 147
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 7 cut(s) 47, 56, 77, 674, 757, 1361, 1460
FalI AAGNNNNNCTT 2 cut(s) 1103, 1135
FaqI GGGAC 2 cut(s) 583, 1106
FatI CATG 7 cut(s) 43, 52, 73, 670, 753, 1357, 1456
FbaI TGATCA 1 cut(s) 843
Fnu4HI GCNGC 6 cut(s) 153, 357, 469, 701, 795, 1043
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 6 cut(s) 153, 357, 469, 701, 795, 1043
FspBI CTAG 3 cut(s) 389, 1136, 1388
FspI TGCGCA 2 cut(s) 1102, 1362
GlaI GCGC 3 cut(s) 205, 1102, 1362
GluI GCNGC 6 cut(s) 153, 357, 469, 701, 795, 1043
GsaI CCCAGC 1 cut(s) 963
GsuI CTGGAG 1 cut(s) 1196
HaeIII GGCC 7 cut(s) 442, 471, 657, 797, 964, 1256, 1455
HapII CCGG 2 cut(s) 187, 1090
HhaI GCGC 3 cut(s) 206, 1103, 1363
Hin1II CATG 7 cut(s) 47, 56, 77, 674, 757, 1361, 1460
Hin6I GCGC 3 cut(s) 204, 1101, 1361
HinP1I GCGC 3 cut(s) 204, 1101, 1361
HincII GTYRAC 1 cut(s) 681
HindII GTYRAC 1 cut(s) 681
HindIII AAGCTT 2 cut(s) 304, 1158
HinfI GANTC 6 cut(s) 58, 311, 519, 542, 1086, 1139
HpaII CCGG 2 cut(s) 187, 1090
HphI GGTGA 4 cut(s) 136, 637, 815, 965
Hpy166II GTNNAC 1 cut(s) 681
Hpy188I TCNGA 4 cut(s) 343, 363, 531, 1015
Hpy188III TCNNGA 7 cut(s) 416, 424, 671, 770, 815, 1136, 1143
Hpy8I GTNNAC 1 cut(s) 681
HpyAV CCTTC 4 cut(s) 208, 944, 1056, 1122
HpyCH4III ACNGT 2 cut(s) 325, 1001
HpyCH4IV ACGT 2 cut(s) 864, 1097
HpyCH4V TGCA 6 cut(s) 65, 110, 163, 256, 779, 1042
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 5 cut(s) 168, 340, 849, 1033, 1370
HpySE526I ACGT 2 cut(s) 864, 1097
Hsp92II CATG 7 cut(s) 47, 56, 77, 674, 757, 1361, 1460
HspAI GCGC 3 cut(s) 204, 1101, 1361
Ksp22I TGATCA 1 cut(s) 843
Kzo9I GATC 6 cut(s) 25, 428, 664, 766, 843, 1132
LmnI GCTCC 1 cut(s) 1291
Lsp1109I GCAGC 3 cut(s) 139, 343, 1054
LweI GCATC 1 cut(s) 1231
MaeI CTAG 3 cut(s) 389, 1136, 1388
MaeII ACGT 2 cut(s) 864, 1097
MaeIII GTNAC 2 cut(s) 852, 1390
MalI GATC 6 cut(s) 27, 430, 666, 768, 845, 1134
MbiI CCGCTC 1 cut(s) 703
MboI GATC 6 cut(s) 25, 428, 664, 766, 843, 1132
MboII GAAGA 6 cut(s) 61, 133, 659, 722, 1050, 1111
MflI RGATCY 3 cut(s) 25, 664, 1132
MhlI GDGCHC 1 cut(s) 120
MlsI TGGCCA 1 cut(s) 1256
MluCI AATT 8 cut(s) 18, 101, 105, 273, 379, 1071, 1115, 1317
MluNI TGGCCA 1 cut(s) 1256
MlyI GAGTC 1 cut(s) 67
Mox20I TGGCCA 1 cut(s) 1256
MroXI GAANNNNTTC 1 cut(s) 1118
MscI TGGCCA 1 cut(s) 1256
MseI TTAA 3 cut(s) 99, 104, 636
Msp20I TGGCCA 1 cut(s) 1256
MspI CCGG 2 cut(s) 187, 1090
MspR9I CCNGG 3 cut(s) 188, 1091, 1175
MvaI CCWGG 1 cut(s) 1175
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 1 cut(s) 169
NciI CCSGG 2 cut(s) 188, 1091
NdeII GATC 6 cut(s) 25, 428, 664, 766, 843, 1132
NlaIII CATG 7 cut(s) 47, 56, 77, 674, 757, 1361, 1460
NlaIV GGNNCC 1 cut(s) 298
NmeAIII GCCGAG 1 cut(s) 1370
NmuCI GTSAC 1 cut(s) 1390
NsbI TGCGCA 2 cut(s) 1102, 1362
PaeR7I CTCGAG 1 cut(s) 815
PagI TCATGA 1 cut(s) 670
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PdmI GAANNNNTTC 1 cut(s) 1118
PfeI GAWTC 5 cut(s) 311, 519, 542, 1086, 1139
PfoI TCCNGGA 2 cut(s) 1089, 1173
PkrI GCNGC 6 cut(s) 154, 358, 470, 702, 796, 1044
PleI GAGTC 1 cut(s) 66
PmaCI CACGTG 1 cut(s) 1098
PmlI CACGTG 1 cut(s) 1098
PpsI GAGTC 1 cut(s) 66
Ppu21I YACGTR 1 cut(s) 1098
PshBI ATTAAT 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 1 cut(s) 1173
PspCI CACGTG 1 cut(s) 1098
PspFI CCCAGC 1 cut(s) 959
PspGI CCWGG 1 cut(s) 1173
PspN4I GGNNCC 1 cut(s) 298
PspPI GGNCC 5 cut(s) 348, 690, 962, 1005, 1453
PsuI RGATCY 3 cut(s) 25, 664, 1132
RsaI GTAC 2 cut(s) 539, 1028
RsaNI GTAC 2 cut(s) 538, 1027
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 3 cut(s) 99, 104, 636
SatI GCNGC 6 cut(s) 153, 357, 469, 701, 795, 1043
Sau3AI GATC 6 cut(s) 25, 428, 664, 766, 843, 1132
Sau96I GGNCC 5 cut(s) 348, 690, 962, 1005, 1453
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 3 cut(s) 188, 1091, 1175
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 1 cut(s) 1231
Sfr274I CTCGAG 1 cut(s) 815
SinI GGWCC 3 cut(s) 348, 690, 1005
SlaI CTCGAG 1 cut(s) 815
SmiI ATTTAAAT 1 cut(s) 100
SmlI CTYRAG 3 cut(s) 578, 659, 815
SmoI CTYRAG 3 cut(s) 578, 659, 815
Sse9I AATT 8 cut(s) 18, 101, 105, 273, 379, 1071, 1115, 1317
SsiI CCGC 5 cut(s) 468, 589, 701, 794, 1332
SspMI CTAG 3 cut(s) 389, 1136, 1388
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 3 cut(s) 186, 1089, 1173
StyI CCWWGG 1 cut(s) 147
SwaI ATTTAAAT 1 cut(s) 100
TaaI ACNGT 2 cut(s) 325, 1001
TaiI ACGT 2 cut(s) 867, 1100
TaqI TCGA 4 cut(s) 114, 417, 816, 1239
TasI AATT 8 cut(s) 18, 101, 105, 273, 379, 1071, 1115, 1317
TauI GCSGC 3 cut(s) 471, 703, 797
TfiI GAWTC 5 cut(s) 311, 519, 542, 1086, 1139
Tru1I TTAA 3 cut(s) 99, 104, 636
Tru9I TTAA 3 cut(s) 99, 104, 636
TscAI CASTG 1 cut(s) 1236
TseFI GTSAC 1 cut(s) 1390
TseI GCWGC 3 cut(s) 152, 356, 1042
Tsp45I GTSAC 1 cut(s) 1390
TspDTI ATGAA 6 cut(s) 60, 90, 147, 659, 715, 990
TspGWI ACGGA 2 cut(s) 461, 929
TspRI CASTG 1 cut(s) 1236
VpaK11BI GGWCC 3 cut(s) 348, 690, 1005
VspI ATTAAT 1 cut(s) 104
XapI RAATTY 3 cut(s) 379, 1115, 1317
XbaI TCTAGA 1 cut(s) 1135
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XhoI CTCGAG 1 cut(s) 815
XmnI GAANNNNTTC 1 cut(s) 1118
XspI CTAG 3 cut(s) 389, 1136, 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.