Domain of unknown function (DUF4220)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1013 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 135, 2054 |
| AccI | GTMKAC | 2 cut(s) | 1157, 1341 |
| AccIII | TCCGGA | 2 cut(s) | 54, 1239 |
| AclWI | GGATC | 4 cut(s) | 975, 1261, 1383, 1472 |
| AcoI | YGGCCR | 2 cut(s) | 1851, 2232 |
| AcsI | RAATTY | 1 cut(s) | 1305 |
| AcuI | CTGAAG | 2 cut(s) | 383, 2086 |
| AcvI | CACGTG | 1 cut(s) | 479 |
| AfaI | GTAC | 4 cut(s) | 164, 696, 1570, 2046 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 135, 539, 2054 |
| AflII | CTTAAG | 3 cut(s) | 70, 524, 1139 |
| AflIII | ACRYGT | 2 cut(s) | 476, 2001 |
| AgsI | TTSAA | 9 cut(s) | 511, 611, 662, 727, 817, 1270, 1444, 1779, 2254 |
| AjnI | CCWGG | 5 cut(s) | 369, 638, 1023, 1527, 2047 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2084, 2116 |
| AleI | CACNNNNGTG | 2 cut(s) | 291, 866 |
| Alw21I | GWGCWC | 1 cut(s) | 837 |
| AlwI | GGATC | 4 cut(s) | 975, 1261, 1383, 1472 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 929, 2347 |
| Aor13HI | TCCGGA | 2 cut(s) | 54, 1239 |
| AoxI | GGCC | 7 cut(s) | 243, 358, 1021, 1845, 1851, 2232, 2431 |
| ApeKI | GCWGC | 6 cut(s) | 182, 627, 1925, 2023, 2242, 2309 |
| ApoI | RAATTY | 1 cut(s) | 1305 |
| AseI | ATTAAT | 2 cut(s) | 203, 821 |
| AspLEI | GCGC | 1 cut(s) | 627 |
| AspS9I | GGNCC | 4 cut(s) | 296, 542, 1401, 2431 |
| AsuC2I | CCSGG | 2 cut(s) | 18, 905 |
| AsuHPI | GGTGA | 6 cut(s) | 233, 853, 1462, 1526, 1600, 1739 |
| AvaII | GGWCC | 3 cut(s) | 296, 542, 1401 |
| BalI | TGGCCA | 2 cut(s) | 1853, 2234 |
| BanI | GGYRCC | 1 cut(s) | 1013 |
| BanII | GRGCYC | 1 cut(s) | 837 |
| BbrPI | CACGTG | 1 cut(s) | 479 |
| BbsI | GAAGAC | 1 cut(s) | 2031 |
| Bbv12I | GWGCWC | 1 cut(s) | 837 |
| BbvI | GCAGC | 6 cut(s) | 169, 639, 1912, 2035, 2229, 2296 |
| BccI | CCATC | 4 cut(s) | 314, 1143, 1239, 2383 |
| BceAI | ACGGC | 1 cut(s) | 762 |
| BciT130I | CCWGG | 5 cut(s) | 371, 640, 1025, 1529, 2049 |
| BciVI | GTATCC | 1 cut(s) | 887 |
| BclI | TGATCA | 1 cut(s) | 1590 |
| BcnI | CCSGG | 2 cut(s) | 18, 905 |
| BfaI | CTAG | 7 cut(s) | 584, 890, 1121, 1224, 1799, 1871, 2439 |
| BfmI | CTRYAG | 3 cut(s) | 930, 1068, 2243 |
| BfrI | CTTAAG | 3 cut(s) | 70, 524, 1139 |
| BfuI | GTATCC | 1 cut(s) | 887 |
| BglII | AGATCT | 2 cut(s) | 742, 2120 |
| BisI | GCNGC | 6 cut(s) | 183, 628, 1926, 2024, 2243, 2310 |
| BlsI | GCNGC | 6 cut(s) | 184, 629, 1927, 2025, 2244, 2311 |
| BmcAI | AGTACT | 1 cut(s) | 1570 |
| Bme1390I | CCNGG | 7 cut(s) | 18, 371, 640, 905, 1025, 1529, 2049 |
| Bme18I | GGWCC | 3 cut(s) | 296, 542, 1401 |
| BmgT120I | GGNCC | 4 cut(s) | 296, 542, 1401, 2431 |
| BmiI | GGNNCC | 1 cut(s) | 1015 |
| BmrFI | CCNGG | 7 cut(s) | 18, 371, 640, 905, 1025, 1529, 2049 |
| BmsI | GCATC | 6 cut(s) | 526, 577, 1082, 1684, 1888, 2209 |
| BpiI | GAAGAC | 1 cut(s) | 2031 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 785, 817 |
| BpmI | CTGGAG | 1 cut(s) | 1511 |
| Bpu10I | CCTNAGC | 1 cut(s) | 344 |
| BpuEI | CTTGAG | 1 cut(s) | 1274 |
| BpuMI | CCSGG | 2 cut(s) | 18, 905 |
| BsaAI | YACGTR | 1 cut(s) | 479 |
| BsaJI | CCNNGG | 5 cut(s) | 144, 217, 289, 864, 1848 |
| BsaWI | WCCGGW | 2 cut(s) | 54, 1239 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2352, 2382 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 135, 539, 2054 |
| Bse1I | ACTGG | 1 cut(s) | 246 |
| Bse3DI | GCAATG | 3 cut(s) | 80, 684, 2317 |
| BseAI | TCCGGA | 2 cut(s) | 54, 1239 |
| BseBI | CCWGG | 5 cut(s) | 371, 640, 1025, 1529, 2049 |
| BseDI | CCNNGG | 5 cut(s) | 144, 217, 289, 864, 1848 |
| BseGI | GGATG | 3 cut(s) | 1073, 1395, 1687 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 135, 539, 2054 |
| BseMI | GCAATG | 3 cut(s) | 80, 684, 2317 |
| BseMII | CTCAG | 3 cut(s) | 816, 1653, 2354 |
| BseNI | ACTGG | 1 cut(s) | 246 |
| BseRI | GAGGAG | 1 cut(s) | 565 |
| BseXI | GCAGC | 6 cut(s) | 169, 639, 1912, 2035, 2229, 2296 |
| BseYI | CCCAGC | 2 cut(s) | 1889, 2312 |
| BsgI | GTGCAG | 1 cut(s) | 942 |
| BshFI | GGCC | 7 cut(s) | 245, 360, 1023, 1847, 1853, 2234, 2433 |
| BshNI | GGYRCC | 1 cut(s) | 1013 |
| BsiHKAI | GWGCWC | 1 cut(s) | 837 |
| BsiSI | CCGG | 4 cut(s) | 18, 55, 904, 1240 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 135, 539, 2054 |
| BsmI | GAATGC | 3 cut(s) | 880, 1265, 1638 |
| BsnI | GGCC | 7 cut(s) | 245, 360, 1023, 1847, 1853, 2234, 2433 |
| Bsp1286I | GDGCHC | 1 cut(s) | 837 |
| Bsp13I | TCCGGA | 2 cut(s) | 54, 1239 |
| Bsp19I | CCATGG | 1 cut(s) | 1848 |
| BspANI | GGCC | 7 cut(s) | 245, 360, 1023, 1847, 1853, 2234, 2433 |
| BspCNI | CTCAG | 3 cut(s) | 815, 1654, 2353 |
| BspEI | TCCGGA | 2 cut(s) | 54, 1239 |
| BspHI | TCATGA | 1 cut(s) | 760 |
| BspLI | GGNNCC | 1 cut(s) | 1015 |
| BspMAI | CTGCAG | 1 cut(s) | 2247 |
| BspPI | GGATC | 4 cut(s) | 975, 1261, 1383, 1472 |
| BspT107I | GGYRCC | 1 cut(s) | 1013 |
| BspTI | CTTAAG | 3 cut(s) | 70, 524, 1139 |
| BsrDI | GCAATG | 3 cut(s) | 80, 684, 2317 |
| BsrI | ACTGG | 1 cut(s) | 246 |
| BssECI | CCNNGG | 5 cut(s) | 144, 217, 289, 864, 1848 |
| BssT1I | CCWWGG | 5 cut(s) | 144, 217, 289, 864, 1848 |
| Bst2UI | CCWGG | 5 cut(s) | 371, 640, 1025, 1529, 2049 |
| Bst4CI | ACNGT | 4 cut(s) | 1069, 1573, 1982, 1992 |
| Bst6I | CTCTTC | 3 cut(s) | 398, 863, 2127 |
| BstAFI | CTTAAG | 3 cut(s) | 70, 524, 1139 |
| BstBAI | YACGTR | 1 cut(s) | 479 |
| BstC8I | GCNNGC | 4 cut(s) | 496, 1409, 1470, 2224 |
| BstDEI | CTNAG | 6 cut(s) | 344, 802, 885, 1662, 2014, 2340 |
| BstDSI | CCRYGG | 1 cut(s) | 1848 |
| BstF5I | GGATG | 3 cut(s) | 1073, 1395, 1687 |
| BstHHI | GCGC | 1 cut(s) | 627 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 179, 191, 529, 774, 886, 1730, 1925 |
| BstNI | CCWGG | 5 cut(s) | 371, 640, 1025, 1529, 2049 |
| BstNSI | RCATGY | 3 cut(s) | 704, 2005, 2226 |
| BstSCI | CCNGG | 7 cut(s) | 16, 369, 638, 903, 1023, 1527, 2047 |
| BstSFI | CTRYAG | 3 cut(s) | 930, 1068, 2243 |
| BstV1I | GCAGC | 6 cut(s) | 169, 639, 1912, 2035, 2229, 2296 |
| BstV2I | GAAGAC | 1 cut(s) | 2031 |
| BstX2I | RGATCY | 5 cut(s) | 742, 967, 1253, 1375, 2120 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1024 |
| BstYI | RGATCY | 5 cut(s) | 742, 967, 1253, 1375, 2120 |
| BsuI | GTATCC | 1 cut(s) | 887 |
| BsuRI | GGCC | 7 cut(s) | 245, 360, 1023, 1847, 1853, 2234, 2433 |
| BtgI | CCRYGG | 1 cut(s) | 1848 |
| BtsCI | GGATG | 3 cut(s) | 1073, 1395, 1687 |
| BtsI | GCAGTG | 5 cut(s) | 28, 309, 696, 1110, 1183 |
| BtsIMutI | CAGTG | 8 cut(s) | 28, 253, 309, 696, 1110, 1183, 1988, 2163 |
| Cac8I | GCNNGC | 4 cut(s) | 496, 1409, 1470, 2224 |
| CaiI | CAGNNNCTG | 2 cut(s) | 929, 2347 |
| CciI | TCATGA | 1 cut(s) | 760 |
| CfoI | GCGC | 1 cut(s) | 627 |
| Cfr13I | GGNCC | 4 cut(s) | 296, 542, 1401, 2431 |
| Csp6I | GTAC | 4 cut(s) | 163, 695, 1569, 2045 |
| CviQI | GTAC | 4 cut(s) | 163, 695, 1569, 2045 |
| DdeI | CTNAG | 6 cut(s) | 344, 802, 885, 1662, 2014, 2340 |
| EaeI | YGGCCR | 2 cut(s) | 1851, 2232 |
| Eam1104I | CTCTTC | 3 cut(s) | 398, 863, 2127 |
| EarI | CTCTTC | 3 cut(s) | 398, 863, 2127 |
| Ecl136II | GAGCTC | 1 cut(s) | 835 |
| Eco130I | CCWWGG | 5 cut(s) | 144, 217, 289, 864, 1848 |
| Eco24I | GRGCYC | 1 cut(s) | 837 |
| Eco47I | GGWCC | 3 cut(s) | 296, 542, 1401 |
| Eco53kI | GAGCTC | 1 cut(s) | 835 |
| Eco57I | CTGAAG | 2 cut(s) | 383, 2086 |
| Eco72I | CACGTG | 1 cut(s) | 479 |
| EcoICRI | GAGCTC | 1 cut(s) | 835 |
| EcoRII | CCWGG | 5 cut(s) | 369, 638, 1023, 1527, 2047 |
| EcoT14I | CCWWGG | 5 cut(s) | 144, 217, 289, 864, 1848 |
| EcoT22I | ATGCAT | 2 cut(s) | 2224, 2228 |
| EcoT38I | GRGCYC | 1 cut(s) | 837 |
| ErhI | CCWWGG | 5 cut(s) | 144, 217, 289, 864, 1848 |
| FbaI | TGATCA | 1 cut(s) | 1590 |
| FblI | GTMKAC | 2 cut(s) | 1157, 1341 |
| Fnu4HI | GCNGC | 6 cut(s) | 183, 628, 1926, 2024, 2243, 2310 |
| FokI | GGATG | 3 cut(s) | 1060, 1402, 1694 |
| FriOI | GRGCYC | 1 cut(s) | 837 |
| Fsp4HI | GCNGC | 6 cut(s) | 183, 628, 1926, 2024, 2243, 2310 |
| FspBI | CTAG | 7 cut(s) | 584, 890, 1121, 1224, 1799, 1871, 2439 |
| GlaI | GCGC | 1 cut(s) | 626 |
| GluI | GCNGC | 6 cut(s) | 183, 628, 1926, 2024, 2243, 2310 |
| GsaI | CCCAGC | 2 cut(s) | 1893, 2316 |
| GsuI | CTGGAG | 1 cut(s) | 1511 |
| HaeIII | GGCC | 7 cut(s) | 245, 360, 1023, 1847, 1853, 2234, 2433 |
| HapII | CCGG | 4 cut(s) | 18, 55, 904, 1240 |
| HhaI | GCGC | 1 cut(s) | 627 |
| Hin6I | GCGC | 1 cut(s) | 625 |
| HinP1I | GCGC | 1 cut(s) | 625 |
| HindIII | AAGCTT | 2 cut(s) | 496, 810 |
| HpaII | CCGG | 4 cut(s) | 18, 55, 904, 1240 |
| HphI | GGTGA | 6 cut(s) | 233, 853, 1462, 1526, 1600, 1739 |
| Hpy166II | GTNNAC | 5 cut(s) | 1158, 1221, 1342, 1576, 2161 |
| Hpy188I | TCNGA | 8 cut(s) | 116, 238, 805, 989, 1060, 1550, 1996, 2072 |
| Hpy8I | GTNNAC | 5 cut(s) | 1158, 1221, 1342, 1576, 2161 |
| HpyAV | CCTTC | 3 cut(s) | 407, 2103, 2248 |
| HpyCH4III | ACNGT | 4 cut(s) | 1069, 1573, 1982, 1992 |
| HpyCH4IV | ACGT | 1 cut(s) | 478 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 179, 191, 529, 774, 886, 1730, 1925 |
| HpyF3I | CTNAG | 6 cut(s) | 344, 802, 885, 1662, 2014, 2340 |
| HpySE526I | ACGT | 1 cut(s) | 478 |
| HspAI | GCGC | 1 cut(s) | 625 |
| Kpn2I | TCCGGA | 2 cut(s) | 54, 1239 |
| Ksp22I | TGATCA | 1 cut(s) | 1590 |
| LmnI | GCTCC | 3 cut(s) | 57, 1823, 2410 |
| Lsp1109I | GCAGC | 6 cut(s) | 169, 639, 1912, 2035, 2229, 2296 |
| LweI | GCATC | 6 cut(s) | 526, 577, 1082, 1684, 1888, 2209 |
| MaeI | CTAG | 7 cut(s) | 584, 890, 1121, 1224, 1799, 1871, 2439 |
| MaeII | ACGT | 1 cut(s) | 478 |
| MaeIII | GTNAC | 6 cut(s) | 4, 221, 282, 402, 1949, 2368 |
| MfeI | CAATTG | 1 cut(s) | 1191 |
| MflI | RGATCY | 5 cut(s) | 742, 967, 1253, 1375, 2120 |
| MhlI | GDGCHC | 1 cut(s) | 837 |
| MlsI | TGGCCA | 2 cut(s) | 1853, 2234 |
| MluNI | TGGCCA | 2 cut(s) | 1853, 2234 |
| MlyI | GAGTC | 3 cut(s) | 262, 1091, 1537 |
| MmeI | TCCRAC | 2 cut(s) | 178, 623 |
| Mox20I | TGGCCA | 2 cut(s) | 1853, 2234 |
| Mph1103I | ATGCAT | 2 cut(s) | 2224, 2228 |
| MroI | TCCGGA | 2 cut(s) | 54, 1239 |
| MscI | TGGCCA | 2 cut(s) | 1853, 2234 |
| MslI | CAYNNNNRTG | 4 cut(s) | 291, 689, 866, 978 |
| Msp20I | TGGCCA | 2 cut(s) | 1853, 2234 |
| MspCI | CTTAAG | 3 cut(s) | 70, 524, 1139 |
| MspI | CCGG | 4 cut(s) | 18, 55, 904, 1240 |
| MspR9I | CCNGG | 7 cut(s) | 18, 371, 640, 905, 1025, 1529, 2049 |
| MunI | CAATTG | 1 cut(s) | 1191 |
| Mva1269I | GAATGC | 3 cut(s) | 880, 1265, 1638 |
| MvaI | CCWGG | 5 cut(s) | 371, 640, 1025, 1529, 2049 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 179, 191, 529, 774, 886, 1730, 1925 |
| NciI | CCSGG | 2 cut(s) | 18, 905 |
| NcoI | CCATGG | 1 cut(s) | 1848 |
| NlaIV | GGNNCC | 1 cut(s) | 1015 |
| NmuCI | GTSAC | 4 cut(s) | 221, 402, 1949, 2368 |
| NsiI | ATGCAT | 2 cut(s) | 2224, 2228 |
| NspI | RCATGY | 3 cut(s) | 704, 2005, 2226 |
| OliI | CACNNNNGTG | 2 cut(s) | 291, 866 |
| PaeI | GCATGC | 1 cut(s) | 2226 |
| PagI | TCATGA | 1 cut(s) | 760 |
| PciI | ACATGT | 1 cut(s) | 2001 |
| PctI | GAATGC | 3 cut(s) | 880, 1265, 1638 |
| PflFI | GACNNNGTC | 1 cut(s) | 1990 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 135, 2054 |
| PfoI | TCCNGGA | 2 cut(s) | 638, 1527 |
| PkrI | GCNGC | 6 cut(s) | 184, 629, 1927, 2025, 2244, 2311 |
| PleI | GAGTC | 3 cut(s) | 262, 1091, 1537 |
| PmaCI | CACGTG | 1 cut(s) | 479 |
| PmlI | CACGTG | 1 cut(s) | 479 |
| PpsI | GAGTC | 3 cut(s) | 262, 1091, 1537 |
| Ppu21I | YACGTR | 1 cut(s) | 479 |
| PscI | ACATGT | 1 cut(s) | 2001 |
| PshBI | ATTAAT | 2 cut(s) | 203, 821 |
| Psp124BI | GAGCTC | 1 cut(s) | 837 |
| Psp6I | CCWGG | 5 cut(s) | 369, 638, 1023, 1527, 2047 |
| PspCI | CACGTG | 1 cut(s) | 479 |
| PspFI | CCCAGC | 2 cut(s) | 1889, 2312 |
| PspGI | CCWGG | 5 cut(s) | 369, 638, 1023, 1527, 2047 |
| PspN4I | GGNNCC | 1 cut(s) | 1015 |
| PspPI | GGNCC | 4 cut(s) | 296, 542, 1401, 2431 |
| PstI | CTGCAG | 1 cut(s) | 2247 |
| PstNI | CAGNNNCTG | 2 cut(s) | 929, 2347 |
| PsuI | RGATCY | 5 cut(s) | 742, 967, 1253, 1375, 2120 |
| PsyI | GACNNNGTC | 1 cut(s) | 1990 |
| RsaI | GTAC | 4 cut(s) | 164, 696, 1570, 2046 |
| RsaNI | GTAC | 4 cut(s) | 163, 695, 1569, 2045 |
| RseI | CAYNNNNRTG | 4 cut(s) | 291, 689, 866, 978 |
| SacI | GAGCTC | 1 cut(s) | 837 |
| SatI | GCNGC | 6 cut(s) | 183, 628, 1926, 2024, 2243, 2310 |
| Sau96I | GGNCC | 4 cut(s) | 296, 542, 1401, 2431 |
| ScaI | AGTACT | 1 cut(s) | 1570 |
| SchI | GAGTC | 3 cut(s) | 262, 1091, 1537 |
| ScrFI | CCNGG | 7 cut(s) | 18, 371, 640, 905, 1025, 1529, 2049 |
| SduI | GDGCHC | 1 cut(s) | 837 |
| SfaNI | GCATC | 6 cut(s) | 526, 577, 1082, 1684, 1888, 2209 |
| SfcI | CTRYAG | 3 cut(s) | 930, 1068, 2243 |
| SinI | GGWCC | 3 cut(s) | 296, 542, 1401 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 291, 689, 866, 978 |
| SmlI | CTYRAG | 4 cut(s) | 70, 524, 1139, 1289 |
| SmoI | CTYRAG | 4 cut(s) | 70, 524, 1139, 1289 |
| SphI | GCATGC | 1 cut(s) | 2226 |
| SspMI | CTAG | 7 cut(s) | 584, 890, 1121, 1224, 1799, 1871, 2439 |
| SstI | GAGCTC | 1 cut(s) | 837 |
| StyD4I | CCNGG | 7 cut(s) | 16, 369, 638, 903, 1023, 1527, 2047 |
| StyI | CCWWGG | 5 cut(s) | 144, 217, 289, 864, 1848 |
| TaaI | ACNGT | 4 cut(s) | 1069, 1573, 1982, 1992 |
| TaiI | ACGT | 1 cut(s) | 481 |
| TaqI | TCGA | 2 cut(s) | 1381, 2130 |
| TatI | WGTACW | 1 cut(s) | 1568 |
| TscAI | CASTG | 8 cut(s) | 28, 253, 316, 696, 1117, 1183, 1995, 2170 |
| TseFI | GTSAC | 4 cut(s) | 221, 402, 1949, 2368 |
| TseI | GCWGC | 6 cut(s) | 182, 627, 1925, 2023, 2242, 2309 |
| Tsp45I | GTSAC | 4 cut(s) | 221, 402, 1949, 2368 |
| TspDTI | ATGAA | 9 cut(s) | 749, 777, 864, 1020, 1033, 1197, 1406, 1426, 1926 |
| TspGWI | ACGGA | 1 cut(s) | 980 |
| TspRI | CASTG | 8 cut(s) | 28, 253, 316, 696, 1117, 1183, 1995, 2170 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1990 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 135, 2054 |
| Vha464I | CTTAAG | 3 cut(s) | 70, 524, 1139 |
| VpaK11BI | GGWCC | 3 cut(s) | 296, 542, 1401 |
| VspI | ATTAAT | 2 cut(s) | 203, 821 |
| XapI | RAATTY | 1 cut(s) | 1305 |
| XbaI | TCTAGA | 1 cut(s) | 1798 |
| XceI | RCATGY | 3 cut(s) | 704, 2005, 2226 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 253, 871 |
| XmiI | GTMKAC | 2 cut(s) | 1157, 1341 |
| XspI | CTAG | 7 cut(s) | 584, 890, 1121, 1224, 1799, 1871, 2439 |
| ZrmI | AGTACT | 1 cut(s) | 1570 |
| Zsp2I | ATGCAT | 2 cut(s) | 2224, 2228 |