Rw5G004550

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000662
Physical Location & Seq
Forward (+)
4757286 .. 4759483
2198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G004550.1

Sequence Viewer

Length: 2142 bp
ATGGTTTCTCCCATTCCTACCAGTGTCAAGAATATATGGGAAAAGTGGAATCTCCGGGGTTTTGTACTCCTCAGCCTCTCATTGCAAACTATTTTAATTCTTGGTGCACCCTTTCGAAAGCGATCACCTAGCTTATTTGTAACCTTGGTTATCTGGTCATCTTACTTGCTTGCTGACTGGGCAGCTAACTTTGCAATTGGCTTAATTGCAAACAGCCAGGGTGATAATCCATTTATTTCAAACCCCAACAACACCGGTCATTTTAATAGCGATCTTCTGGCATTTTGGGCGCCCTTTCTTCTGTTACACCTTGGTGGTCCAGACACAATTACAGCATTTGCTCTTGAGGATAACACATTGTGGCTGAGGCATTTGTTTGGGCTTGTGTTCCAGGTTATAGCTGCGGTTTATGTCTTCATTCAATCATTTCCCAAAAACAAGCTGTGGCTGCCGACGGTTCTCCTATTTCTAGCAGGAATCATCAAGTATGCCGAGCGCACACGTGCTCTGTATCTTGCAAGCTTGGATAATTTTAAGGAGTCCATGATTAAGAAACCTGATCCTGGTCCCAATTATGCAAAGCTGATGGAGGAATACTCTTCGAAAAAAGATGCCAAGCTTCCAACTCATATAGAGTTGACAGCAGAGCGCAGCAAAGATTCCAGGACTACTACTTATGTGGTGGACCCTGGAGATATGAAACATAATATTGCACTGGTTCGCCATGCTTTTCACTTCTTTAACATCTTCAAGGGTCTTATTGTGGATCTCATTTTCAGCTTTCATGAACGATTTGAGAGCCGTGTGTTTTTCCATGACAGAACAGCAGAGGATGCTTTCAAGTTGGTTGCAATTGAGCTCAATTTCATATATGAAGCTCTATTCACCAAGGTGGTTGTTGTGCATTCTAAGAAGGGATACATTTGCCGAGCTATATCCTTCAGTGCAGTTTGCACAGCTCTTGTTTGCTTCCAAAAAATAGAGAAACATGATTTCCATGGGTTTGATGTTGGAATTACCTATATTTTGCTTTTTGGTGCCTTGGCCTTGGATTCAATAGCTGTGTTCATGCTTATATTCTCTGATTGGACTGTTGCTGCTGTGACAAAGTCTTGCCAGGATTCTTGTCTTGCCAATATTCTCAAAAGGTACATCACTCTCAAGCAATCTTGGTGGTTCGTTGCCGAAAATGCAAATCCATATTTGGAGAGGTGTAGGCAACTTCTATTTCGAAGGTGGTACGAATCTGTCTCCATATTCAACTTGATACATTATTCCCTCAAGGAATGTCCAAAATCGAGTCCTAATATTTTTGATTACTTCTTCATGGCCTACATAGAAATCATCTATTTACTGGGTCTTAAGGATCTTCGAGATAAGATCAAATATAGGAAGAACAAGCCACTCAAGAAAGAGCTATGGGAATTCATTTTTGCTCAGTTGAAAGGTAAGTCCATGCTCGCAGATGATCCTGAAACTGCCAGGAGAATAAGTTCGGCCAGAGGTGACTGGATTCTACAGGATAGTGACTGGAATAACTCTACAGATTGTCGTAAATTGCTCCCTTATGTTGTTGATGTTGAATATGATCAGAGCATTCTACTGTGGCACATTGCTACCGAGTTTTCTGTTAAAACTGAAATCGAACATTGTAAGACCTTGTCTGAGTACATGGTATACCTCTTAGTTATGCAGCCTACTATGATGTCGACTGTAGCAGGAATTGGACAGATAAGATTCCGCGATACGTGTGCAGAAGCCAAAAAGTTCTTCAGCAGAAGGGAACTAGGACCAGATGTGGATCAAAAAGAGGCATGTGAGAGAATTCTCGAGGTAAATACAGATGTTCAGCCGGTTGCTGTCAAAGGGGATCGAAGTAAATCCGTGTTGTTTAACGCATGTATTCTAGCGAAAGAACTGAAGAAGATGGACACAGAGAAATGGGATCTGATGAGTCAAGTGTGGGTGGAACTACTAGCATATGCTGCAGGTCACTGCAGAGCAAATGACCATGTCCAATTGCTGAGCAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTAGTGATGGCTCATTTTGGCATAGGGGAGCAGTTCCAGATAAATGAGGGGCATGCAAGAGCAAAACTCATTGTGGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

713

Amino Acids

81.87

Weight (kDa)

8.56

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 425 2.2e-103 Domain of unknown function (DUF4220)
DUF594 PF04578 644 - 694 1.6e-21 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1979
AccB1I GGYRCC 2 cut(s) 289, 1037
AccI GTMKAC 2 cut(s) 1677, 1709
AccII CGCG 1 cut(s) 1743
AciI CCGC 2 cut(s) 404, 1741
AclWI GGATC 7 cut(s) 554, 774, 1374, 1463, 1809, 1878, 1953
AcoI YGGCCR 1 cut(s) 1497
AcsI RAATTY 2 cut(s) 1424, 1824
AcuI CTGAAG 3 cut(s) 925, 1756, 1940
AcvI CACGTG 1 cut(s) 503
AcyI GRCGYC 1 cut(s) 290
AdeI CACNNNGTG 1 cut(s) 360
AfaI GTAC 4 cut(s) 66, 1151, 1241, 1670
AfiI CCNNNNNNNGG 1 cut(s) 563
AflII CTTAAG 1 cut(s) 1361
AflIII ACRYGT 2 cut(s) 500, 1748
AgeI ACCGGT 1 cut(s) 254
AgsI TTSAA 8 cut(s) 240, 422, 751, 841, 1056, 1261, 1444, 1583
AjnI CCWGG 7 cut(s) 216, 390, 562, 662, 688, 1116, 1481
AjuI GAANNNNNNNTTGG 2 cut(s) 1754, 1786
AleI CACNNNNGTG 2 cut(s) 312, 890
AloI GAACNNNNNNTCC 2 cut(s) 1477, 1509
Alw21I GWGCWC 3 cut(s) 109, 508, 861
Alw26I GTCTC 1 cut(s) 1255
Alw44I GTGCAC 1 cut(s) 105
AlwI GGATC 7 cut(s) 554, 774, 1374, 1463, 1809, 1878, 1953
Ama87I CYCGRG 1 cut(s) 1829
AoxI GGCC 3 cut(s) 1044, 1329, 1497
ApaLI GTGCAC 1 cut(s) 105
ApeKI GCWGC 7 cut(s) 182, 401, 448, 651, 1097, 1693, 1985
ApoI RAATTY 2 cut(s) 1424, 1824
AsiGI ACCGGT 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 1492
AspLEI GCGC 3 cut(s) 292, 498, 651
AspS9I GGNCC 4 cut(s) 317, 566, 685, 1790
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 4 cut(s) 117, 233, 877, 1517
AsuII TTCGAA 3 cut(s) 115, 602, 1231
AvaI CYCGRG 1 cut(s) 1829
AvaII GGWCC 4 cut(s) 317, 566, 685, 1790
BaeGI GKGCMC 1 cut(s) 109
BanI GGYRCC 2 cut(s) 289, 1037
BanII GRGCYC 1 cut(s) 861
BbrPI CACGTG 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 3 cut(s) 109, 508, 861
BbvCI CCTCAGC 2 cut(s) 71, 365
BbvI GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1705, 1972
BccI CCATC 3 cut(s) 580, 1921, 2059
BceAI ACGGC 1 cut(s) 786
BcgI CGANNNNNNTGC 2 cut(s) 1733, 1767
BciT130I CCWGG 7 cut(s) 218, 392, 564, 664, 690, 1118, 1483
BciVI GTATCC 1 cut(s) 911
BclI TGATCA 1 cut(s) 1588
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 1255
BfaI CTAG 5 cut(s) 129, 470, 1787, 1907, 1976
BfmI CTRYAG 5 cut(s) 1517, 1542, 1713, 1986, 1996
BfoI RGCGCY 1 cut(s) 293
BfrI CTTAAG 1 cut(s) 1361
BfuAI ACCTGC 1 cut(s) 1979
BfuI GTATCC 1 cut(s) 911
BisI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1694, 1986
BlpI GCTNAGC 1 cut(s) 2024
BlsI GCNGC 7 cut(s) 184, 403, 450, 653, 1099, 1695, 1987
Bme1390I CCNGG 8 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483
Bme18I GGWCC 4 cut(s) 317, 566, 685, 1790
BmeT110I CYCGRG 1 cut(s) 1829
BmgT120I GGNCC 4 cut(s) 317, 566, 685, 1790
BmiI GGNNCC 4 cut(s) 291, 568, 687, 1039
BmrFI CCNGG 8 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483
BmrI ACTGGG 2 cut(s) 187, 1364
BmsI GCATC 2 cut(s) 601, 823
BmuI ACTGGG 2 cut(s) 187, 1364
BpiI GAAGAC 1 cut(s) 406
BplI GAGNNNNNCTC 4 cut(s) 581, 613, 2109, 2141
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 2 cut(s) 71, 365
Bpu1102I GCTNAGC 1 cut(s) 2024
Bpu14I TTCGAA 3 cut(s) 115, 602, 1231
BpuEI CTTGAG 4 cut(s) 365, 1145, 1265, 1391
BpuMI CCSGG 1 cut(s) 56
BsaAI YACGTR 2 cut(s) 503, 1749
BsaBI GATNNNNATC 1 cut(s) 1800
BsaHI GRCGYC 1 cut(s) 290
BsaJI CCNNGG 9 cut(s) 55, 144, 217, 310, 688, 888, 997, 1041, 1047
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 4 cut(s) 1477, 1507, 2028, 2058
Bsc4I CCNNNNNNNGG 1 cut(s) 563
Bse118I RCCGGY 2 cut(s) 254, 1852
Bse1I ACTGG 6 cut(s) 21, 182, 720, 1359, 1514, 1535
Bse3DI GCAATG 2 cut(s) 80, 1611
Bse8I GATNNNNATC 1 cut(s) 1800
BseBI CCWGG 7 cut(s) 218, 392, 564, 664, 690, 1118, 1483
BseDI CCNNGG 9 cut(s) 55, 144, 217, 310, 688, 888, 997, 1041, 1047
BseGI GGATG 1 cut(s) 838
BseJI GATNNNNATC 1 cut(s) 1800
BseLI CCNNNNNNNGG 1 cut(s) 563
BseMI GCAATG 2 cut(s) 80, 1611
BseMII CTCAG 5 cut(s) 85, 356, 1451, 1656, 2015
BseNI ACTGG 6 cut(s) 21, 182, 720, 1359, 1514, 1535
BseRI GAGGAG 1 cut(s) 59
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1705, 1972
BsgI GTGCAG 2 cut(s) 966, 1773
Bsh1236I CGCG 1 cut(s) 1743
BshFI GGCC 3 cut(s) 1046, 1331, 1499
BshNI GGYRCC 2 cut(s) 289, 1037
BshTI ACCGGT 1 cut(s) 254
BsiHKAI GWGCWC 3 cut(s) 109, 508, 861
BsiHKCI CYCGRG 1 cut(s) 1829
BsiSI CCGG 3 cut(s) 55, 255, 1853
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 1 cut(s) 563
BsmAI GTCTC 1 cut(s) 1255
BsmFI GGGAC 1 cut(s) 552
BsmI GAATGC 2 cut(s) 904, 1596
BsnI GGCC 3 cut(s) 1046, 1331, 1499
BsoBI CYCGRG 1 cut(s) 1829
Bsp119I TTCGAA 3 cut(s) 115, 602, 1231
Bsp1286I GDGCHC 3 cut(s) 109, 508, 861
Bsp1720I GCTNAGC 1 cut(s) 2024
Bsp19I CCATGG 1 cut(s) 997
BspACI CCGC 2 cut(s) 404, 1741
BspANI GGCC 3 cut(s) 1046, 1331, 1499
BspCNI CTCAG 5 cut(s) 84, 357, 1450, 1657, 2016
BspFNI CGCG 1 cut(s) 1743
BspHI TCATGA 1 cut(s) 784
BspLI GGNNCC 4 cut(s) 291, 568, 687, 1039
BspMAI CTGCAG 2 cut(s) 1990, 2000
BspMI ACCTGC 1 cut(s) 1979
BspPI GGATC 7 cut(s) 554, 774, 1374, 1463, 1809, 1878, 1953
BspT104I TTCGAA 3 cut(s) 115, 602, 1231
BspT107I GGYRCC 2 cut(s) 289, 1037
BspTI CTTAAG 1 cut(s) 1361
BsrDI GCAATG 2 cut(s) 80, 1611
BsrFI RCCGGY 2 cut(s) 254, 1852
BsrI ACTGG 6 cut(s) 21, 182, 720, 1359, 1514, 1535
BssAI RCCGGY 2 cut(s) 254, 1852
BssECI CCNNGG 9 cut(s) 55, 144, 217, 310, 688, 888, 997, 1041, 1047
BssNAI GTATAC 1 cut(s) 1678
BssNI GRCGYC 1 cut(s) 290
BssT1I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
Bst1107I GTATAC 1 cut(s) 1678
Bst2UI CCWGG 7 cut(s) 218, 392, 564, 664, 690, 1118, 1483
Bst4CI ACNGT 4 cut(s) 457, 1093, 1605, 1714
Bst6I CTCTTC 1 cut(s) 604
BstACI GRCGYC 1 cut(s) 290
BstAFI CTTAAG 1 cut(s) 1361
BstAPI GCANNNNNTGC 2 cut(s) 833, 1985
BstBAI YACGTR 2 cut(s) 503, 1749
BstBI TTCGAA 3 cut(s) 115, 602, 1231
BstC8I GCNNGC 4 cut(s) 171, 520, 1461, 2112
BstDEI CTNAG 7 cut(s) 71, 365, 909, 1437, 1665, 1684, 2024
BstDSI CCRYGG 1 cut(s) 997
BstF5I GGATG 1 cut(s) 838
BstFNI CGCG 1 cut(s) 1743
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 3 cut(s) 292, 498, 651
BstMAI GTCTC 1 cut(s) 1255
BstMWI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 1985
BstNI CCWGG 7 cut(s) 218, 392, 564, 664, 690, 1118, 1483
BstNSI RCATGY 3 cut(s) 1818, 1902, 2114
BstSCI CCNGG 8 cut(s) 54, 216, 390, 562, 662, 688, 1116, 1481
BstSFI CTRYAG 5 cut(s) 1517, 1542, 1713, 1986, 1996
BstSLI GKGCMC 1 cut(s) 109
BstUI CGCG 1 cut(s) 1743
BstV1I GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1705, 1972
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 3 cut(s) 766, 1366, 1945
BstYI RGATCY 3 cut(s) 766, 1366, 1945
BstZ17I GTATAC 1 cut(s) 1678
BsuI GTATCC 1 cut(s) 911
BsuRI GGCC 3 cut(s) 1046, 1331, 1499
BtgI CCRYGG 1 cut(s) 997
BtsCI GGATG 1 cut(s) 838
BtsI GCAGTG 1 cut(s) 1993
BtsIMutI CAGTG 4 cut(s) 28, 713, 949, 1993
BveI ACCTGC 1 cut(s) 1979
Cac8I GCNNGC 4 cut(s) 171, 520, 1461, 2112
CciI TCATGA 1 cut(s) 784
CfoI GCGC 3 cut(s) 292, 498, 651
Cfr10I RCCGGY 2 cut(s) 254, 1852
Cfr13I GGNCC 4 cut(s) 317, 566, 685, 1790
Csp6I GTAC 4 cut(s) 65, 1150, 1240, 1669
CspAI ACCGGT 1 cut(s) 254
CspCI CAANNNNNGTGG 2 cut(s) 1154, 1189
CviQI GTAC 4 cut(s) 65, 1150, 1240, 1669
DdeI CTNAG 7 cut(s) 71, 365, 909, 1437, 1665, 1684, 2024
DinI GGCGCC 1 cut(s) 291
DraIII CACNNNGTG 1 cut(s) 360
EaeI YGGCCR 1 cut(s) 1497
Eam1104I CTCTTC 1 cut(s) 604
EarI CTCTTC 1 cut(s) 604
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
Eco24I GRGCYC 1 cut(s) 861
Eco47I GGWCC 4 cut(s) 317, 566, 685, 1790
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 3 cut(s) 925, 1756, 1940
Eco72I CACGTG 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 1829
EcoICRI GAGCTC 1 cut(s) 859
EcoRI GAATTC 2 cut(s) 1424, 1824
EcoRII CCWGG 7 cut(s) 216, 390, 562, 662, 688, 1116, 1481
EcoT14I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
EcoT38I GRGCYC 1 cut(s) 861
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
FaqI GGGAC 1 cut(s) 552
FauNDI CATATG 1 cut(s) 1981
FbaI TGATCA 1 cut(s) 1588
FblI GTMKAC 2 cut(s) 1677, 1709
Fnu4HI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1694, 1986
FokI GGATG 1 cut(s) 845
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1694, 1986
FspBI CTAG 5 cut(s) 129, 470, 1787, 1907, 1976
GlaI GCGC 3 cut(s) 291, 497, 650
GluI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1694, 1986
GsuI CTGGAG 1 cut(s) 711
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 3 cut(s) 1046, 1331, 1499
HapII CCGG 3 cut(s) 55, 255, 1853
HhaI GCGC 3 cut(s) 292, 498, 651
Hin1I GRCGYC 1 cut(s) 290
Hin6I GCGC 3 cut(s) 290, 496, 649
HinP1I GCGC 3 cut(s) 290, 496, 649
HincII GTYRAC 2 cut(s) 639, 1710
HindII GTYRAC 2 cut(s) 639, 1710
HindIII AAGCTT 2 cut(s) 520, 617
HpaII CCGG 3 cut(s) 55, 255, 1853
HphI GGTGA 4 cut(s) 117, 233, 877, 1517
Hpy166II GTNNAC 5 cut(s) 107, 639, 685, 1678, 1710
Hpy188I TCNGA 4 cut(s) 1084, 1593, 1666, 1950
Hpy188III TCNNGA 9 cut(s) 28, 320, 344, 785, 1373, 1408, 1472, 1829, 2095
Hpy8I GTNNAC 5 cut(s) 107, 639, 685, 1678, 1710
Hpy99I CGWCG 1 cut(s) 457
HpyAV CCTTC 4 cut(s) 907, 949, 1227, 1773
HpyCH4III ACNGT 4 cut(s) 457, 1093, 1605, 1714
HpyCH4IV ACGT 2 cut(s) 502, 1748
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 1985
HpyF3I CTNAG 7 cut(s) 71, 365, 909, 1437, 1665, 1684, 2024
HpySE526I ACGT 2 cut(s) 502, 1748
Hsp92I GRCGYC 1 cut(s) 290
HspAI GCGC 3 cut(s) 290, 496, 649
KasI GGCGCC 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 1588
LmnI GCTCC 2 cut(s) 1566, 2086
Lsp1109I GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1705, 1972
LweI GCATC 2 cut(s) 601, 823
MaeI CTAG 5 cut(s) 129, 470, 1787, 1907, 1976
MaeII ACGT 2 cut(s) 502, 1748
MaeIII GTNAC 7 cut(s) 139, 303, 1102, 1505, 1526, 1991, 2044
MfeI CAATTG 3 cut(s) 195, 852, 2018
MflI RGATCY 3 cut(s) 766, 1366, 1945
MhlI GDGCHC 3 cut(s) 109, 508, 861
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 3 cut(s) 548, 1309, 1963
MmeI TCCRAC 2 cut(s) 647, 991
MroXI GAANNNNTTC 1 cut(s) 1492
MseI TTAA 9 cut(s) 95, 203, 264, 534, 549, 741, 1362, 1632, 1893
MslI CAYNNNNRTG 2 cut(s) 312, 890
MspCI CTTAAG 1 cut(s) 1361
MspI CCGG 3 cut(s) 55, 255, 1853
MspR9I CCNGG 8 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483
MunI CAATTG 3 cut(s) 195, 852, 2018
Mva1269I GAATGC 2 cut(s) 904, 1596
MvaI CCWGG 7 cut(s) 218, 392, 564, 664, 690, 1118, 1483
MvnI CGCG 1 cut(s) 1743
MwoI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 1985
NarI GGCGCC 1 cut(s) 290
NciI CCSGG 1 cut(s) 56
NcoI CCATGG 1 cut(s) 997
NdeI CATATG 1 cut(s) 1981
NlaIV GGNNCC 4 cut(s) 291, 568, 687, 1039
NmeAIII GCCGAG 2 cut(s) 517, 953
NmuCI GTSAC 5 cut(s) 1102, 1505, 1526, 1991, 2044
NspI RCATGY 3 cut(s) 1818, 1902, 2114
NspV TTCGAA 3 cut(s) 115, 602, 1231
OliI CACNNNNGTG 2 cut(s) 312, 890
PaeI GCATGC 1 cut(s) 2114
PaeR7I CTCGAG 1 cut(s) 1829
PagI TCATGA 1 cut(s) 784
PctI GAATGC 2 cut(s) 904, 1596
PdmI GAANNNNTTC 1 cut(s) 1492
PfeI GAWTC 8 cut(s) 49, 477, 659, 1052, 1121, 1244, 1513, 1737
PflFI GACNNNGTC 3 cut(s) 1108, 1660, 2012
PfoI TCCNGGA 1 cut(s) 662
PinAI ACCGGT 1 cut(s) 254
PkrI GCNGC 7 cut(s) 184, 403, 450, 653, 1099, 1695, 1987
PleI GAGTC 3 cut(s) 547, 1308, 1962
PluTI GGCGCC 1 cut(s) 293
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 3 cut(s) 547, 1308, 1962
Ppu21I YACGTR 2 cut(s) 503, 1749
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 7 cut(s) 216, 390, 562, 662, 688, 1116, 1481
PspCI CACGTG 1 cut(s) 503
PspGI CCWGG 7 cut(s) 216, 390, 562, 662, 688, 1116, 1481
PspN4I GGNNCC 4 cut(s) 291, 568, 687, 1039
PspPI GGNCC 4 cut(s) 317, 566, 685, 1790
PstI CTGCAG 2 cut(s) 1990, 2000
PsuI RGATCY 3 cut(s) 766, 1366, 1945
PsyI GACNNNGTC 3 cut(s) 1108, 1660, 2012
RsaI GTAC 4 cut(s) 66, 1151, 1241, 1670
RsaNI GTAC 4 cut(s) 65, 1150, 1240, 1669
RseI CAYNNNNRTG 2 cut(s) 312, 890
SacI GAGCTC 1 cut(s) 861
SalI GTCGAC 1 cut(s) 1708
SaqAI TTAA 9 cut(s) 95, 203, 264, 534, 549, 741, 1362, 1632, 1893
SatI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1694, 1986
Sau96I GGNCC 4 cut(s) 317, 566, 685, 1790
SchI GAGTC 3 cut(s) 548, 1309, 1963
ScrFI CCNGG 8 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483
SduI GDGCHC 3 cut(s) 109, 508, 861
SfaNI GCATC 2 cut(s) 601, 823
SfcI CTRYAG 5 cut(s) 1517, 1542, 1713, 1986, 1996
SfoI GGCGCC 1 cut(s) 291
Sfr274I CTCGAG 1 cut(s) 1829
SfuI TTCGAA 3 cut(s) 115, 602, 1231
SinI GGWCC 4 cut(s) 317, 566, 685, 1790
SlaI CTCGAG 1 cut(s) 1829
SmiMI CAYNNNNRTG 2 cut(s) 312, 890
SmlI CTYRAG 6 cut(s) 344, 1160, 1280, 1361, 1406, 1829
SmoI CTYRAG 6 cut(s) 344, 1160, 1280, 1361, 1406, 1829
SphI GCATGC 1 cut(s) 2114
SsiI CCGC 2 cut(s) 404, 1741
SspDI GGCGCC 1 cut(s) 289
SspI AATATT 3 cut(s) 709, 1138, 1309
SspMI CTAG 5 cut(s) 129, 470, 1787, 1907, 1976
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 8 cut(s) 54, 216, 390, 562, 662, 688, 1116, 1481
StyI CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
TaaI ACNGT 4 cut(s) 457, 1093, 1605, 1714
TaiI ACGT 2 cut(s) 505, 1751
TaqI TCGA 9 cut(s) 115, 602, 1231, 1298, 1372, 1644, 1709, 1830, 1873
TatI WGTACW 2 cut(s) 64, 1668
TfiI GAWTC 8 cut(s) 49, 477, 659, 1052, 1121, 1244, 1513, 1737
Tru1I TTAA 9 cut(s) 95, 203, 264, 534, 549, 741, 1362, 1632, 1893
Tru9I TTAA 9 cut(s) 95, 203, 264, 534, 549, 741, 1362, 1632, 1893
TscAI CASTG 4 cut(s) 28, 720, 949, 2000
TseFI GTSAC 5 cut(s) 1102, 1505, 1526, 1991, 2044
TseI GCWGC 7 cut(s) 182, 401, 448, 651, 1097, 1693, 1985
Tsp45I GTSAC 5 cut(s) 1102, 1505, 1526, 1991, 2044
TspDTI ATGAA 9 cut(s) 406, 713, 773, 801, 856, 888, 1057, 1315, 1417
TspGWI ACGGA 1 cut(s) 1873
TspRI CASTG 4 cut(s) 28, 720, 949, 2000
Tth111I GACNNNGTC 3 cut(s) 1108, 1660, 2012
Vha464I CTTAAG 1 cut(s) 1361
VneI GTGCAC 1 cut(s) 105
VpaK11BI GGWCC 4 cut(s) 317, 566, 685, 1790
XapI RAATTY 2 cut(s) 1424, 1824
XceI RCATGY 3 cut(s) 1818, 1902, 2114
XhoI CTCGAG 1 cut(s) 1829
XmiI GTMKAC 2 cut(s) 1677, 1709
XmnI GAANNNNTTC 1 cut(s) 1492
XspI CTAG 5 cut(s) 129, 470, 1787, 1907, 1976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.