MD17G1136800.v1.1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000662
Physical Location & Seq
Forward (+)
12241071 .. 12244543
3473 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1136800.v1.1.491

Sequence Viewer

Length: 2220 bp
ATGGCCGGTAAGGTTAACCCCATTCCTAACAGCATCAAGAAGCTATGGGACAAGTGGAACCTCAGGGGCTTTATCCTCTTAAGCCTCACATTGCAGACCCTTTTAATTATTTTTGCACCCGCGCGAAAGCGGACGCCAAACATGGGGATAATGTTCATCGTTTGGTCATCTTACTTGCTCGCTGACTGGGCTGCTAACTTTGCCATTGGATTAATCTCAAACAGCCAAGGCGACCATGCAGGTGACGACTATCCTGGTGATCTTCTGGCATTTTGGGCTCCCTTTCTTTTGTTGCACCTTGGCGGTCCAGACACCATTACTGCGTTTGCGCTTGAAGATAACACTCTGTGGCTCAGGCACTTTCTTGGCCTCATATTCCAAGTTGCAGCTGCTGTGTATGTCTTCATTCGATCCTTCCCCAAAAACAAACTATGGCTCCCAACCGGCCTCCTTTTTCTTGCTGGAATCATCAAGTATGCTGAGCGCACACGGTCCTTGTATAGTGCAAGCTTGGATAATTTCAAGGAAGCCATGATAAAAGAAGCTGATCCAGGACCAAATTACGCAAAGCTTATGGAAGAGTACTCCTCGAGAAAAGATTCCCAACTTCCAACTCACATAGAACTCACAGCAGAACGCAGCAAGGAATCTAGGACGGTGACTTACGTGATAGGCAAAGATGAGCTGGACAGTGATATTGCAGTGGTGAAACATGCTCATTATTTCTATCAAATCTTCAAGGGTCTAATTGTGGATCTCATTTTCAGCTTCCATGAACGCCAGGAGAGTCGTGCAGTCTTCCTTGAAAGAACACCGAAAGAAGCATTTAAATTAATTGCAGTCGAGCTCAACTTTATGTATGAAGCTCTCTTCACCAAGGCTGCTGTGGTGCATACTAAGCCAGCATACATTCTCCGGGTGGTTTCTTTCAGTGCGATTGTCATAGCCCTTGTTTTCTTCTATAGATTAGAGAAAGAGGGTTTGCACTCGTTTGATGTTAGAATTACCTATACCTTGCTGTTTGGTGCCATAAGCTTGGATTCAATAGCTATTGTCATGCTTACATTCTCAGATTGGACCATTGCTGCTCTGAATAAGTTTTGGCAAAATTCTGGTCTAGCTAAAACTATTTTCGGAAAGTATCTCGATTTCAAGAGATCGCGGTGGTGGCCTAAGGCAAAAACAAGAAATGAGGGCGGCCAAATTCCTAAAACAAAAGCAACAAAATGTTTGGAGTGGATTAGGAGAATCCTATTTCGGAGGTGGTACGAATCTGTCTCTACATTCAACTTGATACATTACTCCCTCAAGGAGAAGCGGAAAATATCCCCGACATGGTATGACTACTTTGGCATTGGCTACATTAAAGTCATCACCTTATTTGGCCTCAAGGATCTTCATGATAAGTTGATATATAGGACCAAAAAGCCACTCGCTGAAGAGTTATGGGTTTTTATTTTTGAGCAGTTGATAAGTAAATCCATGCTCGCTGATGATCCTGAAACTGCAAGGAAAATAGGTGCGGCCAGAGGTGATTGGGTTCTCGAGGGTAGCGATTGGGATAGTAAGGTGATCACTAAGTTACTATCTTACGTTGTTGATGTGGAATATGACAAGAGCATTTTATTGTGGCACATTGCTACAGAATTGTGTTTCAACGATGACAAAAGTGAAGCTGAAGGTGAAGCTGGGCCTGAAGTTAGTGATGAAACTAAAAAAAGTAAAGAACACAGTAAGACCATATCTGATTATATGTTGTACCTCTTAGTTATGCAGCCTTCTATGATGTCTTCTATAACAGGAATTGGACAAATAAGATTCCGGGACACATGCGCAGAAGCCAAGAAATTCTTCAGCAGAAGGGATCTAGGATCCGTACAAGTGAAAGAAGCTTGTGAAAAACTCCTGGAGGTAAATACAGATGTTGAGCCTGTTGCTGTCAAAGGGGATAGAAGCAAATCTGTGTTGTTCGATGCTTGTATTCTGGCCAAAGAGTTGAGAAAATTGAAGGACAAGAAATGGGAGCTGATGAGCGAAGTGTGGGTGGAAATTATGTCTTATACTGCAAGTCATTGCCGAGCGAATGACCATGCGCAACTGCTGAGCAAAGGTGGAGAGCTAGTGACTTTTGTTTGGTTATTGATGGCTCATTTTGGCATAGGAGAGCAGTTCCAAATAAATGAGGGCCATGCGAGAGCAAAACTCATCGTGGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

740

Amino Acids

84.64

Weight (kDa)

8.89

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 434 1e-102 Domain of unknown function (DUF4220)
DUF594 PF04578 670 - 720 5.6e-23 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 230
Acc16I TGCGCA 2 cut(s) 1834, 2094
Acc36I ACCTGC 1 cut(s) 230
AccB1I GGYRCC 1 cut(s) 1025
AccII CGCG 3 cut(s) 122, 124, 1162
AciI CCGC 7 cut(s) 120, 130, 303, 1162, 1197, 1318, 1523
AclWI GGATC 8 cut(s) 405, 542, 762, 1401, 1490, 1866, 1872, 1879
AcoI YGGCCR 4 cut(s) 3, 1198, 1524, 1986
AcsI RAATTY 3 cut(s) 1108, 1203, 1847
AcuI CTGAAG 4 cut(s) 1458, 1698, 1716, 1837
AcyI GRCGYC 1 cut(s) 134
AdeI CACNNNGTG 1 cut(s) 348
AfaI GTAC 4 cut(s) 584, 1268, 1760, 1878
AfiI CCNNNNNNNGG 2 cut(s) 143, 1335
AflII CTTAAG 1 cut(s) 79
AgsI TTSAA 9 cut(s) 335, 523, 739, 806, 1044, 1153, 1288, 1657, 2008
AjnI CCWGG 4 cut(s) 253, 550, 780, 1905
AjuI GAANNNNNNNTTGG 2 cut(s) 1835, 1867
Alw21I GWGCWC 1 cut(s) 849
Alw26I GTCTC 1 cut(s) 1282
AlwI GGATC 8 cut(s) 405, 542, 762, 1401, 1490, 1866, 1872, 1879
AlwNI CAGNNNCTG 1 cut(s) 392
Ama87I CYCGRG 2 cut(s) 589, 1544
ApeKI GCWGC 7 cut(s) 191, 386, 389, 639, 881, 1085, 1774
ApoI RAATTY 3 cut(s) 1108, 1203, 1847
ArsI GACNNNNNNTTYG 2 cut(s) 1099, 1131
AseI ATTAAT 2 cut(s) 212, 833
Asp700I GAANNNNTTC 2 cut(s) 598, 1850
AspLEI GCGC 5 cut(s) 124, 331, 486, 1835, 2095
AspS9I GGNCC 7 cut(s) 305, 492, 554, 1077, 1419, 1691, 2185
AsuC2I CCSGG 2 cut(s) 917, 1823
AsuHPI GGTGA 9 cut(s) 254, 269, 670, 718, 865, 1366, 1544, 1582, 1694
AvaI CYCGRG 2 cut(s) 589, 1544
AvaII GGWCC 5 cut(s) 305, 492, 554, 1077, 1419
AxyI CCTNAGG 2 cut(s) 62, 1173
BalI TGGCCA 1 cut(s) 1988
BamHI GGATCC 1 cut(s) 1871
BanI GGYRCC 1 cut(s) 1025
BanII GRGCYC 2 cut(s) 280, 849
BbsI GAAGAC 3 cut(s) 394, 790, 1782
Bbv12I GWGCWC 1 cut(s) 849
BbvI GCAGC 7 cut(s) 178, 376, 398, 651, 868, 1072, 1786
BccI CCATC 1 cut(s) 2137
BciT130I CCWGG 4 cut(s) 255, 552, 782, 1907
BclI TGATCA 1 cut(s) 1572
BcnI CCSGG 2 cut(s) 917, 1823
BcoDI GTCTC 1 cut(s) 1282
BfaI CTAG 4 cut(s) 651, 1118, 1868, 2120
BfmI CTRYAG 2 cut(s) 961, 1641
BfrI CTTAAG 1 cut(s) 79
BfuAI ACCTGC 1 cut(s) 230
BisI GCNGC 9 cut(s) 192, 387, 390, 640, 882, 1086, 1198, 1524, 1775
BlpI GCTNAGC 2 cut(s) 480, 2102
BlsI GCNGC 9 cut(s) 193, 388, 391, 641, 883, 1087, 1199, 1525, 1776
BmcAI AGTACT 1 cut(s) 584
Bme1390I CCNGG 6 cut(s) 255, 552, 782, 917, 1823, 1907
Bme18I GGWCC 5 cut(s) 305, 492, 554, 1077, 1419
BmeT110I CYCGRG 2 cut(s) 589, 1544
BmgT120I GGNCC 7 cut(s) 305, 492, 554, 1077, 1419, 1691, 2185
BmiI GGNNCC 5 cut(s) 59, 279, 437, 1027, 1873
BmrFI CCNGG 6 cut(s) 255, 552, 782, 917, 1823, 1907
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 2 cut(s) 42, 1963
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 3 cut(s) 394, 790, 1782
BplI GAGNNNNNCTC 4 cut(s) 572, 604, 2187, 2219
BpmI CTGGAG 1 cut(s) 1928
Bpu10I CCTNAGC 1 cut(s) 353
Bpu1102I GCTNAGC 2 cut(s) 480, 2102
BpuEI CTTGAG 2 cut(s) 1292, 1373
BpuMI CCSGG 2 cut(s) 917, 1823
BsaAI YACGTR 1 cut(s) 667
BsaHI GRCGYC 1 cut(s) 134
BsaJI CCNNGG 3 cut(s) 226, 298, 876
BsaXI ACNNNNNCTCC 4 cut(s) 420, 450, 2106, 2136
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 1335
Bse118I RCCGGY 2 cut(s) 5, 443
Bse1I ACTGG 1 cut(s) 191
Bse21I CCTNAGG 2 cut(s) 62, 1173
Bse3DI GCAATG 4 cut(s) 89, 1080, 1635, 2071
BseBI CCWGG 4 cut(s) 255, 552, 782, 1907
BseDI CCNNGG 3 cut(s) 226, 298, 876
BseLI CCNNNNNNNGG 2 cut(s) 143, 1335
BseMI GCAATG 4 cut(s) 89, 1080, 1635, 2071
BseMII CTCAG 5 cut(s) 76, 367, 471, 1083, 2093
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 1 cut(s) 577
BseXI GCAGC 7 cut(s) 178, 376, 398, 651, 868, 1072, 1786
BseYI CCCAGC 1 cut(s) 1688
BsgI GTGCAG 1 cut(s) 813
Bsh1236I CGCG 3 cut(s) 122, 124, 1162
BshNI GGYRCC 1 cut(s) 1025
BsiHKAI GWGCWC 1 cut(s) 849
BsiHKCI CYCGRG 2 cut(s) 589, 1544
BsiSI CCGG 4 cut(s) 6, 444, 916, 1822
BslFI GGGAC 2 cut(s) 62, 1838
BslI CCNNNNNNNGG 2 cut(s) 143, 1335
BsmAI GTCTC 1 cut(s) 1282
BsmFI GGGAC 2 cut(s) 62, 1838
BsoBI CYCGRG 2 cut(s) 589, 1544
Bsp1286I GDGCHC 2 cut(s) 280, 849
Bsp1720I GCTNAGC 2 cut(s) 480, 2102
BspACI CCGC 7 cut(s) 120, 130, 303, 1162, 1197, 1318, 1523
BspCNI CTCAG 5 cut(s) 75, 366, 472, 1082, 2094
BspFNI CGCG 3 cut(s) 122, 124, 1162
BspHI TCATGA 1 cut(s) 1399
BspLI GGNNCC 5 cut(s) 59, 279, 437, 1027, 1873
BspMI ACCTGC 1 cut(s) 230
BspPI GGATC 8 cut(s) 405, 542, 762, 1401, 1490, 1866, 1872, 1879
BspT107I GGYRCC 1 cut(s) 1025
BspTI CTTAAG 1 cut(s) 79
BsrDI GCAATG 4 cut(s) 89, 1080, 1635, 2071
BsrFI RCCGGY 2 cut(s) 5, 443
BsrI ACTGG 1 cut(s) 191
BssAI RCCGGY 2 cut(s) 5, 443
BssECI CCNNGG 3 cut(s) 226, 298, 876
BssNI GRCGYC 1 cut(s) 134
BssT1I CCWWGG 3 cut(s) 226, 298, 876
Bst2UI CCWGG 4 cut(s) 255, 552, 782, 1907
Bst4CI ACNGT 4 cut(s) 492, 658, 692, 1733
Bst6I CTCTTC 3 cut(s) 573, 875, 1434
BstACI GRCGYC 1 cut(s) 134
BstAFI CTTAAG 1 cut(s) 79
BstBAI YACGTR 1 cut(s) 667
BstC8I GCNNGC 4 cut(s) 180, 508, 903, 1488
BstDEI CTNAG 9 cut(s) 62, 353, 480, 897, 1069, 1173, 1578, 1765, 2102
BstFNI CGCG 3 cut(s) 122, 124, 1162
BstHHI GCGC 5 cut(s) 124, 331, 486, 1835, 2095
BstMAI GTCTC 1 cut(s) 1282
BstMWI GCNNNNNNNGC 5 cut(s) 188, 200, 275, 898, 1168
BstNI CCWGG 4 cut(s) 255, 552, 782, 1907
BstNSI RCATGY 2 cut(s) 716, 1833
BstSCI CCNGG 6 cut(s) 253, 550, 780, 915, 1821, 1905
BstSFI CTRYAG 2 cut(s) 961, 1641
BstUI CGCG 3 cut(s) 122, 124, 1162
BstV1I GCAGC 7 cut(s) 178, 376, 398, 651, 868, 1072, 1786
BstV2I GAAGAC 3 cut(s) 394, 790, 1782
BstX2I RGATCY 4 cut(s) 754, 1393, 1864, 1871
BstXI CCANNNNNNTGG 1 cut(s) 1036
BstYI RGATCY 4 cut(s) 754, 1393, 1864, 1871
Bsu36I CCTNAGG 2 cut(s) 62, 1173
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 3 cut(s) 697, 708, 937
BveI ACCTGC 1 cut(s) 230
Cac8I GCNNGC 4 cut(s) 180, 508, 903, 1488
CaiI CAGNNNCTG 1 cut(s) 392
CciI TCATGA 1 cut(s) 1399
CfoI GCGC 5 cut(s) 124, 331, 486, 1835, 2095
Cfr10I RCCGGY 2 cut(s) 5, 443
Cfr13I GGNCC 7 cut(s) 305, 492, 554, 1077, 1419, 1691, 2185
CseI GACGC 1 cut(s) 142
Csp6I GTAC 4 cut(s) 583, 1267, 1759, 1877
CviQI GTAC 4 cut(s) 583, 1267, 1759, 1877
DdeI CTNAG 9 cut(s) 62, 353, 480, 897, 1069, 1173, 1578, 1765, 2102
DraI TTTAAA 1 cut(s) 829
DraIII CACNNNGTG 1 cut(s) 348
EaeI YGGCCR 4 cut(s) 3, 1198, 1524, 1986
Eam1104I CTCTTC 3 cut(s) 573, 875, 1434
EarI CTCTTC 3 cut(s) 573, 875, 1434
Ecl136II GAGCTC 1 cut(s) 847
Eco130I CCWWGG 3 cut(s) 226, 298, 876
Eco24I GRGCYC 2 cut(s) 280, 849
Eco47I GGWCC 5 cut(s) 305, 492, 554, 1077, 1419
Eco53kI GAGCTC 1 cut(s) 847
Eco57I CTGAAG 4 cut(s) 1458, 1698, 1716, 1837
Eco81I CCTNAGG 2 cut(s) 62, 1173
Eco88I CYCGRG 2 cut(s) 589, 1544
EcoICRI GAGCTC 1 cut(s) 847
EcoRII CCWGG 4 cut(s) 253, 550, 780, 1905
EcoT14I CCWWGG 3 cut(s) 226, 298, 876
EcoT38I GRGCYC 2 cut(s) 280, 849
ErhI CCWWGG 3 cut(s) 226, 298, 876
FalI AAGNNNNNCTT 2 cut(s) 1835, 1867
FaqI GGGAC 2 cut(s) 62, 1838
FauI CCCGC 1 cut(s) 127
FbaI TGATCA 1 cut(s) 1572
Fnu4HI GCNGC 9 cut(s) 192, 387, 390, 640, 882, 1086, 1198, 1524, 1775
FriOI GRGCYC 2 cut(s) 280, 849
Fsp4HI GCNGC 9 cut(s) 192, 387, 390, 640, 882, 1086, 1198, 1524, 1775
FspBI CTAG 4 cut(s) 651, 1118, 1868, 2120
FspI TGCGCA 2 cut(s) 1834, 2094
GlaI GCGC 5 cut(s) 123, 330, 485, 1834, 2094
GluI GCNGC 9 cut(s) 192, 387, 390, 640, 882, 1086, 1198, 1524, 1775
GsaI CCCAGC 1 cut(s) 1692
GsuI CTGGAG 1 cut(s) 1928
HapII CCGG 4 cut(s) 6, 444, 916, 1822
HgaI GACGC 1 cut(s) 142
HhaI GCGC 5 cut(s) 124, 331, 486, 1835, 2095
Hin1I GRCGYC 1 cut(s) 134
Hin6I GCGC 5 cut(s) 122, 329, 484, 1833, 2093
HinP1I GCGC 5 cut(s) 122, 329, 484, 1833, 2093
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 4 cut(s) 508, 569, 1033, 1890
HinfI GANTC 8 cut(s) 465, 599, 647, 787, 1040, 1248, 1271, 1818
HpaI GTTAAC 1 cut(s) 16
HpaII CCGG 4 cut(s) 6, 444, 916, 1822
HphI GGTGA 9 cut(s) 254, 269, 670, 718, 865, 1366, 1544, 1582, 1694
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 5 cut(s) 1072, 1092, 1136, 1260, 1747
Hpy188III TCNNGA 8 cut(s) 37, 308, 591, 1145, 1153, 1400, 1499, 1544
Hpy8I GTNNAC 1 cut(s) 16
HpyAV CCTTC 5 cut(s) 424, 1673, 1788, 1854, 2002
HpyCH4III ACNGT 4 cut(s) 492, 658, 692, 1733
HpyCH4IV ACGT 2 cut(s) 666, 1593
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 200, 275, 898, 1168
HpyF3I CTNAG 9 cut(s) 62, 353, 480, 897, 1069, 1173, 1578, 1765, 2102
HpySE526I ACGT 2 cut(s) 666, 1593
Hsp92I GRCGYC 1 cut(s) 134
HspAI GCGC 5 cut(s) 122, 329, 484, 1833, 2093
Ksp22I TGATCA 1 cut(s) 1572
KspAI GTTAAC 1 cut(s) 16
LmnI GCTCC 3 cut(s) 283, 441, 2023
Lsp1109I GCAGC 7 cut(s) 178, 376, 398, 651, 868, 1072, 1786
LweI GCATC 2 cut(s) 42, 1963
MaeI CTAG 4 cut(s) 651, 1118, 1868, 2120
MaeII ACGT 2 cut(s) 666, 1593
MaeIII GTNAC 4 cut(s) 242, 658, 1581, 2122
MflI RGATCY 4 cut(s) 754, 1393, 1864, 1871
MhlI GDGCHC 2 cut(s) 280, 849
MlsI TGGCCA 1 cut(s) 1988
MluNI TGGCCA 1 cut(s) 1988
MlyI GAGTC 1 cut(s) 796
MmeI TCCRAC 1 cut(s) 635
Mox20I TGGCCA 1 cut(s) 1988
MroXI GAANNNNTTC 2 cut(s) 598, 1850
MscI TGGCCA 1 cut(s) 1988
MseI TTAA 7 cut(s) 15, 80, 104, 212, 828, 833, 1365
MslI CAYNNNNRTG 1 cut(s) 240
Msp20I TGGCCA 1 cut(s) 1988
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 79
MspI CCGG 4 cut(s) 6, 444, 916, 1822
MspR9I CCNGG 6 cut(s) 255, 552, 782, 917, 1823, 1907
MvaI CCWGG 4 cut(s) 255, 552, 782, 1907
MvnI CGCG 3 cut(s) 122, 124, 1162
MwoI GCNNNNNNNGC 5 cut(s) 188, 200, 275, 898, 1168
NciI CCSGG 2 cut(s) 917, 1823
NlaIV GGNNCC 5 cut(s) 59, 279, 437, 1027, 1873
NmeAIII GCCGAG 1 cut(s) 2102
NmuCI GTSAC 3 cut(s) 242, 658, 2122
NsbI TGCGCA 2 cut(s) 1834, 2094
NspI RCATGY 2 cut(s) 716, 1833
PaeR7I CTCGAG 2 cut(s) 589, 1544
PagI TCATGA 1 cut(s) 1399
PaqCI CACCTGC 1 cut(s) 230
PcsI WCGNNNNNNNCGW 1 cut(s) 1551
PdmI GAANNNNTTC 2 cut(s) 598, 1850
PfeI GAWTC 7 cut(s) 465, 599, 647, 1040, 1248, 1271, 1818
PfoI TCCNGGA 3 cut(s) 550, 1821, 1905
PkrI GCNGC 9 cut(s) 193, 388, 391, 641, 883, 1087, 1199, 1525, 1776
PleI GAGTC 1 cut(s) 795
PpsI GAGTC 1 cut(s) 795
Ppu21I YACGTR 1 cut(s) 667
PshBI ATTAAT 2 cut(s) 212, 833
Psp124BI GAGCTC 1 cut(s) 849
Psp6I CCWGG 4 cut(s) 253, 550, 780, 1905
PspFI CCCAGC 1 cut(s) 1688
PspGI CCWGG 4 cut(s) 253, 550, 780, 1905
PspN4I GGNNCC 5 cut(s) 59, 279, 437, 1027, 1873
PspPI GGNCC 7 cut(s) 305, 492, 554, 1077, 1419, 1691, 2185
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 4 cut(s) 754, 1393, 1864, 1871
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 4 cut(s) 584, 1268, 1760, 1878
RsaNI GTAC 4 cut(s) 583, 1267, 1759, 1877
RseI CAYNNNNRTG 1 cut(s) 240
SacI GAGCTC 1 cut(s) 849
SaqAI TTAA 7 cut(s) 15, 80, 104, 212, 828, 833, 1365
SatI GCNGC 9 cut(s) 192, 387, 390, 640, 882, 1086, 1198, 1524, 1775
Sau96I GGNCC 7 cut(s) 305, 492, 554, 1077, 1419, 1691, 2185
ScaI AGTACT 1 cut(s) 584
SchI GAGTC 1 cut(s) 796
ScrFI CCNGG 6 cut(s) 255, 552, 782, 917, 1823, 1907
SduI GDGCHC 2 cut(s) 280, 849
SfaNI GCATC 2 cut(s) 42, 1963
SfcI CTRYAG 2 cut(s) 961, 1641
Sfr274I CTCGAG 2 cut(s) 589, 1544
SinI GGWCC 5 cut(s) 305, 492, 554, 1077, 1419
SlaI CTCGAG 2 cut(s) 589, 1544
SmiI ATTTAAAT 1 cut(s) 829
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 5 cut(s) 79, 589, 1307, 1388, 1544
SmoI CTYRAG 5 cut(s) 79, 589, 1307, 1388, 1544
SsiI CCGC 7 cut(s) 120, 130, 303, 1162, 1197, 1318, 1523
SspMI CTAG 4 cut(s) 651, 1118, 1868, 2120
SstI GAGCTC 1 cut(s) 849
StyD4I CCNGG 6 cut(s) 253, 550, 780, 915, 1821, 1905
StyI CCWWGG 3 cut(s) 226, 298, 876
SwaI ATTTAAAT 1 cut(s) 829
TaaI ACNGT 4 cut(s) 492, 658, 692, 1733
TaiI ACGT 2 cut(s) 669, 1596
TaqI TCGA 6 cut(s) 409, 590, 843, 1146, 1545, 1971
TatI WGTACW 1 cut(s) 582
TauI GCSGC 2 cut(s) 1200, 1526
TfiI GAWTC 7 cut(s) 465, 599, 647, 1040, 1248, 1271, 1818
Tru1I TTAA 7 cut(s) 15, 80, 104, 212, 828, 833, 1365
Tru9I TTAA 7 cut(s) 15, 80, 104, 212, 828, 833, 1365
TscAI CASTG 3 cut(s) 697, 708, 937
TseFI GTSAC 3 cut(s) 242, 658, 2122
TseI GCWGC 7 cut(s) 191, 386, 389, 639, 881, 1085, 1774
Tsp45I GTSAC 3 cut(s) 242, 658, 2122
TspDTI ATGAA 6 cut(s) 145, 394, 789, 876, 1388, 1722
TspGWI ACGGA 1 cut(s) 1864
TspRI CASTG 3 cut(s) 697, 708, 937
Vha464I CTTAAG 1 cut(s) 79
VpaK11BI GGWCC 5 cut(s) 305, 492, 554, 1077, 1419
VspI ATTAAT 2 cut(s) 212, 833
XapI RAATTY 3 cut(s) 1108, 1203, 1847
XceI RCATGY 2 cut(s) 716, 1833
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XhoI CTCGAG 2 cut(s) 589, 1544
XmnI GAANNNNTTC 2 cut(s) 598, 1850
XspI CTAG 4 cut(s) 651, 1118, 1868, 2120
ZrmI AGTACT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.