Rh5DG043800

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000662
Physical Location & Seq
Forward (+)
3632824 .. 3635016
2193 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG043800.1

Sequence Viewer

Length: 2193 bp
ATGGTTTCTCCCATTCCTACCAGTGTCAAGAATATATGGGAAAAGTGGAATCTCCGGGGTTTTGTACTCCTCAGCCTCTCATTGCAAACTATTTTAATTCTTGGTGCACCCTTTCGAAAGCGATCACCTAGCTTATTTGTAACCTTGGTTATCTGGTCATCTTACTTGCTTGCTGACTGGGCAGCTAACTTTGCAATTGGCTTAATTGCAAACAGCCAGGGTGATAATCCATTTATTCCAAACCCCAACAACACCGGTCATTTTAATAGCGATCTTCTGGCATTTTGGGCACCCTTTCTTCTGTTACACCTTGGTGGTCCAGACACAATTACAGCATTTGCTCTTGAGGATAACACATTGTGGCTAAGGCACTTGTTTGGGCTTGTGTTCCAGGTTATAGCTGCGGTTTATGTCTTCATTCAATCATTTCCCAAAAACAAGCTGTGGCTGCCGACGGTACTCCTATTTCTTGCAGGAATCATCAAGTATGCCGAGCGCACACGTGCTCTGTATCTTGCAAGCTTGGATAATTTTAAGGAGTCCATGATTAAGAAACCTGATCCTGGTCCCAATTATGCAAAGCTGATGGAGGAATACTCTTCGAAAAAAGATGCCAAGCTTCCAACTCATATAGAGTTGACAGCAGAGCGCAGCAAAGATTCCAGGACTACTACTTATGTGGTGGACCCTAAGGATATGGAACATAATATTGCACTGGTTCGCCATGCTTTTCACTTCTTTAACATCTTCAAGGGTCTTATTGTGGATCTCATTTTCAGCTTTCACGAACGATTTGAGAGCCGTGTGTTTTTCCATGACAGAACAGCAGAGGATGCTTTCAAGTTGGTTGCAATTGAGCTCAATTTCATATATGAAGCTCTATTCACCAAGGTGGTTGTTGTGCATTCTAAGAAGGGATACATTTGCCGAGCTATATCCTTCAGTGCAGTTTGCACAGCTCTTGTTTGCTTCCAAAAAATAGAGAAACATGATTTCCATGGGTTTGATGTTGGAATTACCTATATTTTGCTTCTTGGTGCCTTGGCCTTGGATTCAATAGCTGTGTTCATGCTTATATTCTCTGATTGGACTGTTGCTGCTGTGAAGAAGTCTTGCCAGGATTCTTGTCTTGCCAATATTCTCAAAAGGTACATCACTCTCAAGCAATCTTGGTGGTTCGTTGCCGAAAATGCAAATCCATATTTGGAGAGGTGTAGGCAACTTCTATTTCGAAGGTGGTACGAATCTGTCTCCATATTCAACTTGATACATTATTCCCTCAAGGAATGTCCAAAATCGAGTCCTAATATTTTTGATTACTTCTTCATGGCCTACATAAAAATCATCTACTTACTGGGTCTTAAGGATCTTCGAGATAAGATCAAATATAGGAAGAGCAAGCCACTCAAGAAAGAGCTATGGGAATTCATTTTTGCTCAGTTGAAAGGTAAGTCCATGCTCGCAGATGATCCTGAAACTGCCAGGAGAATAAGTTCGGCCAGAGGTGACTGGATTCTACAGGATAGTGACTGGAATAACTCTACAGATTGTCGTAAATTGCTCCCTTATGTTGTTGATGTTGAATATGATCAGAGCATTCTACTGTGGCACATTGCTACCGAGTTTTGTTATAATGAAATTAAACCCGAGTTTTTTAAAGAGGATCAAACAAACAAGGCTGTTCTCACTAAAATCGAACATTGTAAGACCTTGTCTGAGTACATGGTATACCTCTTAGTTATGCAGCCTACTATGATGTCTACTGTAGCAGGAATTGGACAAATAAGATTCCGCGATACGTGTGCAGAAGCGAAAAAGTTCTTCAGCAGAAGGGAACTAGGACCAGATGTGGATCAAAAAGACGCATGTGATAGAATTCTCGAGGTAAATACAGATGTTCAGCCGGTTGCTGTCAAAGGGGATCGCAGTAAATCTGTGTTGTTTGATGCATGTATTCTAGCGAAAGAACTGAAGAAGATGGACACAGAGAAATGGGATCTCATGAGTCAAGTGTGGGTGGAACTACTAGCATATTCTGCAGGTCACTGCAGAGCAAATGACCATGTCCAATTGCTGAGCAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTAGTGATGGCTCATTTTGGCATAGGGGAGCAGTTCTTGATAAATGAGGGGCATGCAAGAGCAAAACTCATTGTGGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

730

Amino Acids

83.97

Weight (kDa)

8.6

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 425 6.8e-104 Domain of unknown function (DUF4220)
DUF594 PF04578 661 - 711 3.7e-21 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1632
Acc36I ACCTGC 1 cut(s) 2030
AccB1I GGYRCC 2 cut(s) 289, 1037
AccI GTMKAC 2 cut(s) 1728, 1760
AccII CGCG 1 cut(s) 1794
AciI CCGC 2 cut(s) 404, 1792
AclWI GGATC 8 cut(s) 554, 774, 1374, 1463, 1671, 1860, 1929, 2004
AcoI YGGCCR 1 cut(s) 1497
AcsI RAATTY 2 cut(s) 1424, 1875
AcuI CTGAAG 3 cut(s) 925, 1807, 1991
AcvI CACGTG 1 cut(s) 503
AdeI CACNNNGTG 1 cut(s) 360
AfaI GTAC 5 cut(s) 66, 459, 1151, 1241, 1721
AfiI CCNNNNNNNGG 1 cut(s) 563
AflII CTTAAG 1 cut(s) 1361
AflIII ACRYGT 2 cut(s) 500, 1799
AgeI ACCGGT 1 cut(s) 254
AgsI TTSAA 7 cut(s) 422, 751, 841, 1056, 1261, 1444, 1583
AjnI CCWGG 6 cut(s) 216, 390, 562, 662, 1116, 1481
AleI CACNNNNGTG 2 cut(s) 312, 890
AloI GAACNNNNNNTCC 2 cut(s) 1477, 1509
Alw21I GWGCWC 3 cut(s) 109, 508, 861
Alw26I GTCTC 1 cut(s) 1255
Alw44I GTGCAC 1 cut(s) 105
AlwI GGATC 8 cut(s) 554, 774, 1374, 1463, 1671, 1860, 1929, 2004
Ama87I CYCGRG 2 cut(s) 1646, 1880
AoxI GGCC 3 cut(s) 1044, 1329, 1497
ApaLI GTGCAC 1 cut(s) 105
ApeKI GCWGC 6 cut(s) 182, 401, 448, 651, 1097, 1744
ApoI RAATTY 2 cut(s) 1424, 1875
AsiGI ACCGGT 1 cut(s) 254
Asp700I GAANNNNTTC 2 cut(s) 1492, 1817
AspLEI GCGC 2 cut(s) 498, 651
AspS9I GGNCC 4 cut(s) 317, 566, 685, 1841
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 4 cut(s) 117, 233, 877, 1517
AsuII TTCGAA 3 cut(s) 115, 602, 1231
AvaI CYCGRG 2 cut(s) 1646, 1880
AvaII GGWCC 4 cut(s) 317, 566, 685, 1841
AxyI CCTNAGG 1 cut(s) 690
BaeGI GKGCMC 2 cut(s) 109, 292
BanI GGYRCC 2 cut(s) 289, 1037
BanII GRGCYC 1 cut(s) 861
BbrPI CACGTG 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 3 cut(s) 109, 508, 861
BbvCI CCTCAGC 1 cut(s) 71
BbvI GCAGC 6 cut(s) 194, 388, 435, 663, 1084, 1756
BccI CCATC 3 cut(s) 580, 1972, 2110
BceAI ACGGC 1 cut(s) 786
BcgI CGANNNNNNTGC 2 cut(s) 1784, 1818
BciT130I CCWGG 6 cut(s) 218, 392, 564, 664, 1118, 1483
BciVI GTATCC 1 cut(s) 911
BclI TGATCA 1 cut(s) 1588
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 1255
BfaI CTAG 4 cut(s) 129, 1838, 1958, 2027
BfmI CTRYAG 5 cut(s) 1517, 1542, 1764, 2037, 2047
BfrI CTTAAG 1 cut(s) 1361
BfuAI ACCTGC 1 cut(s) 2030
BfuI GTATCC 1 cut(s) 911
BisI GCNGC 6 cut(s) 183, 402, 449, 652, 1098, 1745
BlpI GCTNAGC 1 cut(s) 2075
BlsI GCNGC 6 cut(s) 184, 403, 450, 653, 1099, 1746
Bme1390I CCNGG 7 cut(s) 56, 218, 392, 564, 664, 1118, 1483
Bme18I GGWCC 4 cut(s) 317, 566, 685, 1841
BmeT110I CYCGRG 2 cut(s) 1646, 1880
BmgT120I GGNCC 4 cut(s) 317, 566, 685, 1841
BmiI GGNNCC 4 cut(s) 291, 568, 687, 1039
BmrFI CCNGG 7 cut(s) 56, 218, 392, 564, 664, 1118, 1483
BmrI ACTGGG 2 cut(s) 187, 1364
BmsI GCATC 3 cut(s) 601, 823, 1936
BmuI ACTGGG 2 cut(s) 187, 1364
BpiI GAAGAC 1 cut(s) 406
BplI GAGNNNNNCTC 4 cut(s) 581, 613, 2160, 2192
Bpu10I CCTNAGC 2 cut(s) 71, 365
Bpu1102I GCTNAGC 1 cut(s) 2075
Bpu14I TTCGAA 3 cut(s) 115, 602, 1231
BpuEI CTTGAG 4 cut(s) 365, 1145, 1265, 1391
BpuMI CCSGG 1 cut(s) 56
BsaAI YACGTR 2 cut(s) 503, 1800
BsaBI GATNNNNATC 1 cut(s) 1851
BsaJI CCNNGG 8 cut(s) 55, 144, 217, 310, 888, 997, 1041, 1047
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 4 cut(s) 1477, 1507, 2079, 2109
Bsc4I CCNNNNNNNGG 1 cut(s) 563
Bse118I RCCGGY 2 cut(s) 254, 1903
Bse1I ACTGG 6 cut(s) 21, 182, 720, 1359, 1514, 1535
Bse21I CCTNAGG 1 cut(s) 690
Bse3DI GCAATG 2 cut(s) 80, 1611
Bse8I GATNNNNATC 1 cut(s) 1851
BseBI CCWGG 6 cut(s) 218, 392, 564, 664, 1118, 1483
BseDI CCNNGG 8 cut(s) 55, 144, 217, 310, 888, 997, 1041, 1047
BseGI GGATG 1 cut(s) 838
BseJI GATNNNNATC 1 cut(s) 1851
BseLI CCNNNNNNNGG 1 cut(s) 563
BseMI GCAATG 2 cut(s) 80, 1611
BseMII CTCAG 4 cut(s) 85, 1451, 1707, 2066
BseNI ACTGG 6 cut(s) 21, 182, 720, 1359, 1514, 1535
BseRI GAGGAG 1 cut(s) 59
BseSI GKGCMC 2 cut(s) 109, 292
BseXI GCAGC 6 cut(s) 194, 388, 435, 663, 1084, 1756
BsgI GTGCAG 2 cut(s) 966, 1824
Bsh1236I CGCG 1 cut(s) 1794
BshFI GGCC 3 cut(s) 1046, 1331, 1499
BshNI GGYRCC 2 cut(s) 289, 1037
BshTI ACCGGT 1 cut(s) 254
BsiHKAI GWGCWC 3 cut(s) 109, 508, 861
BsiHKCI CYCGRG 2 cut(s) 1646, 1880
BsiSI CCGG 3 cut(s) 55, 255, 1904
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 1 cut(s) 563
BsmAI GTCTC 1 cut(s) 1255
BsmFI GGGAC 1 cut(s) 552
BsmI GAATGC 2 cut(s) 904, 1596
BsnI GGCC 3 cut(s) 1046, 1331, 1499
BsoBI CYCGRG 2 cut(s) 1646, 1880
Bsp119I TTCGAA 3 cut(s) 115, 602, 1231
Bsp1286I GDGCHC 4 cut(s) 109, 292, 508, 861
Bsp1720I GCTNAGC 1 cut(s) 2075
Bsp19I CCATGG 1 cut(s) 997
BspACI CCGC 2 cut(s) 404, 1792
BspANI GGCC 3 cut(s) 1046, 1331, 1499
BspCNI CTCAG 4 cut(s) 84, 1450, 1708, 2067
BspFNI CGCG 1 cut(s) 1794
BspHI TCATGA 1 cut(s) 2001
BspLI GGNNCC 4 cut(s) 291, 568, 687, 1039
BspMAI CTGCAG 2 cut(s) 2041, 2051
BspMI ACCTGC 1 cut(s) 2030
BspPI GGATC 8 cut(s) 554, 774, 1374, 1463, 1671, 1860, 1929, 2004
BspQI GCTCTTC 1 cut(s) 1388
BspT104I TTCGAA 3 cut(s) 115, 602, 1231
BspT107I GGYRCC 2 cut(s) 289, 1037
BspTI CTTAAG 1 cut(s) 1361
BsrDI GCAATG 2 cut(s) 80, 1611
BsrFI RCCGGY 2 cut(s) 254, 1903
BsrI ACTGG 6 cut(s) 21, 182, 720, 1359, 1514, 1535
BssAI RCCGGY 2 cut(s) 254, 1903
BssECI CCNNGG 8 cut(s) 55, 144, 217, 310, 888, 997, 1041, 1047
BssNAI GTATAC 1 cut(s) 1729
BssT1I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
Bst1107I GTATAC 1 cut(s) 1729
Bst2UI CCWGG 6 cut(s) 218, 392, 564, 664, 1118, 1483
Bst4CI ACNGT 4 cut(s) 457, 1093, 1605, 1765
Bst6I CTCTTC 2 cut(s) 604, 1388
BstAFI CTTAAG 1 cut(s) 1361
BstAPI GCANNNNNTGC 2 cut(s) 833, 2036
BstBAI YACGTR 2 cut(s) 503, 1800
BstBI TTCGAA 3 cut(s) 115, 602, 1231
BstC8I GCNNGC 5 cut(s) 171, 520, 1400, 1461, 2163
BstDEI CTNAG 8 cut(s) 71, 365, 690, 909, 1437, 1716, 1735, 2075
BstDSI CCRYGG 1 cut(s) 997
BstF5I GGATG 1 cut(s) 838
BstFNI CGCG 1 cut(s) 1794
BstHHI GCGC 2 cut(s) 498, 651
BstMAI GTCTC 1 cut(s) 1255
BstMWI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 2036
BstNI CCWGG 6 cut(s) 218, 392, 564, 664, 1118, 1483
BstNSI RCATGY 3 cut(s) 1869, 1953, 2165
BstSCI CCNGG 7 cut(s) 54, 216, 390, 562, 662, 1116, 1481
BstSFI CTRYAG 5 cut(s) 1517, 1542, 1764, 2037, 2047
BstSLI GKGCMC 2 cut(s) 109, 292
BstUI CGCG 1 cut(s) 1794
BstV1I GCAGC 6 cut(s) 194, 388, 435, 663, 1084, 1756
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 3 cut(s) 766, 1366, 1996
BstYI RGATCY 3 cut(s) 766, 1366, 1996
BstZ17I GTATAC 1 cut(s) 1729
Bsu36I CCTNAGG 1 cut(s) 690
BsuI GTATCC 1 cut(s) 911
BsuRI GGCC 3 cut(s) 1046, 1331, 1499
BtgI CCRYGG 1 cut(s) 997
BtsCI GGATG 1 cut(s) 838
BtsI GCAGTG 1 cut(s) 2044
BtsIMutI CAGTG 4 cut(s) 28, 713, 949, 2044
BveI ACCTGC 1 cut(s) 2030
Cac8I GCNNGC 5 cut(s) 171, 520, 1400, 1461, 2163
CciI TCATGA 1 cut(s) 2001
CfoI GCGC 2 cut(s) 498, 651
Cfr10I RCCGGY 2 cut(s) 254, 1903
Cfr13I GGNCC 4 cut(s) 317, 566, 685, 1841
CseI GACGC 1 cut(s) 1871
Csp6I GTAC 5 cut(s) 65, 458, 1150, 1240, 1720
CspAI ACCGGT 1 cut(s) 254
CspCI CAANNNNNGTGG 2 cut(s) 1154, 1189
CviQI GTAC 5 cut(s) 65, 458, 1150, 1240, 1720
DdeI CTNAG 8 cut(s) 71, 365, 690, 909, 1437, 1716, 1735, 2075
DraI TTTAAA 1 cut(s) 1657
DraIII CACNNNGTG 1 cut(s) 360
EaeI YGGCCR 1 cut(s) 1497
Eam1104I CTCTTC 2 cut(s) 604, 1388
EarI CTCTTC 2 cut(s) 604, 1388
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
Eco24I GRGCYC 1 cut(s) 861
Eco47I GGWCC 4 cut(s) 317, 566, 685, 1841
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 3 cut(s) 925, 1807, 1991
Eco72I CACGTG 1 cut(s) 503
Eco81I CCTNAGG 1 cut(s) 690
Eco88I CYCGRG 2 cut(s) 1646, 1880
EcoICRI GAGCTC 1 cut(s) 859
EcoRI GAATTC 2 cut(s) 1424, 1875
EcoRII CCWGG 6 cut(s) 216, 390, 562, 662, 1116, 1481
EcoT14I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
EcoT22I ATGCAT 1 cut(s) 1951
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
FaqI GGGAC 1 cut(s) 552
FbaI TGATCA 1 cut(s) 1588
FblI GTMKAC 2 cut(s) 1728, 1760
Fnu4HI GCNGC 6 cut(s) 183, 402, 449, 652, 1098, 1745
FokI GGATG 1 cut(s) 845
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 6 cut(s) 183, 402, 449, 652, 1098, 1745
FspBI CTAG 4 cut(s) 129, 1838, 1958, 2027
GlaI GCGC 2 cut(s) 497, 650
GluI GCNGC 6 cut(s) 183, 402, 449, 652, 1098, 1745
HaeIII GGCC 3 cut(s) 1046, 1331, 1499
HapII CCGG 3 cut(s) 55, 255, 1904
HgaI GACGC 1 cut(s) 1871
HhaI GCGC 2 cut(s) 498, 651
Hin6I GCGC 2 cut(s) 496, 649
HinP1I GCGC 2 cut(s) 496, 649
HincII GTYRAC 1 cut(s) 639
HindII GTYRAC 1 cut(s) 639
HindIII AAGCTT 2 cut(s) 520, 617
HpaII CCGG 3 cut(s) 55, 255, 1904
HphI GGTGA 4 cut(s) 117, 233, 877, 1517
Hpy166II GTNNAC 5 cut(s) 107, 639, 685, 1729, 1761
Hpy188I TCNGA 3 cut(s) 1084, 1593, 1717
Hpy8I GTNNAC 5 cut(s) 107, 639, 685, 1729, 1761
Hpy99I CGWCG 1 cut(s) 457
HpyAV CCTTC 4 cut(s) 907, 949, 1227, 1824
HpyCH4III ACNGT 4 cut(s) 457, 1093, 1605, 1765
HpyCH4IV ACGT 2 cut(s) 502, 1799
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 2036
HpyF3I CTNAG 8 cut(s) 71, 365, 690, 909, 1437, 1716, 1735, 2075
HpySE526I ACGT 2 cut(s) 502, 1799
HspAI GCGC 2 cut(s) 496, 649
Ksp22I TGATCA 1 cut(s) 1588
LguI GCTCTTC 1 cut(s) 1388
LmnI GCTCC 2 cut(s) 1566, 2137
Lsp1109I GCAGC 6 cut(s) 194, 388, 435, 663, 1084, 1756
LweI GCATC 3 cut(s) 601, 823, 1936
MaeI CTAG 4 cut(s) 129, 1838, 1958, 2027
MaeII ACGT 2 cut(s) 502, 1799
MaeIII GTNAC 6 cut(s) 139, 303, 1505, 1526, 2042, 2095
MfeI CAATTG 3 cut(s) 195, 852, 2069
MflI RGATCY 3 cut(s) 766, 1366, 1996
MhlI GDGCHC 4 cut(s) 109, 292, 508, 861
MlyI GAGTC 3 cut(s) 548, 1309, 2014
MmeI TCCRAC 2 cut(s) 647, 991
Mph1103I ATGCAT 1 cut(s) 1951
MroXI GAANNNNTTC 2 cut(s) 1492, 1817
MseI TTAA 9 cut(s) 95, 203, 264, 534, 549, 741, 1362, 1641, 1656
MslI CAYNNNNRTG 2 cut(s) 312, 890
MspCI CTTAAG 1 cut(s) 1361
MspI CCGG 3 cut(s) 55, 255, 1904
MspR9I CCNGG 7 cut(s) 56, 218, 392, 564, 664, 1118, 1483
MunI CAATTG 3 cut(s) 195, 852, 2069
Mva1269I GAATGC 2 cut(s) 904, 1596
MvaI CCWGG 6 cut(s) 218, 392, 564, 664, 1118, 1483
MvnI CGCG 1 cut(s) 1794
MwoI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 2036
NciI CCSGG 1 cut(s) 56
NcoI CCATGG 1 cut(s) 997
NlaIV GGNNCC 4 cut(s) 291, 568, 687, 1039
NmeAIII GCCGAG 2 cut(s) 517, 953
NmuCI GTSAC 4 cut(s) 1505, 1526, 2042, 2095
NsiI ATGCAT 1 cut(s) 1951
NspI RCATGY 3 cut(s) 1869, 1953, 2165
NspV TTCGAA 3 cut(s) 115, 602, 1231
OliI CACNNNNGTG 2 cut(s) 312, 890
PaeI GCATGC 1 cut(s) 2165
PaeR7I CTCGAG 1 cut(s) 1880
PagI TCATGA 1 cut(s) 2001
PciSI GCTCTTC 1 cut(s) 1388
PctI GAATGC 2 cut(s) 904, 1596
PdmI GAANNNNTTC 2 cut(s) 1492, 1817
PfeI GAWTC 8 cut(s) 49, 477, 659, 1052, 1121, 1244, 1513, 1788
PflFI GACNNNGTC 2 cut(s) 1711, 2063
PfoI TCCNGGA 1 cut(s) 662
PinAI ACCGGT 1 cut(s) 254
PkrI GCNGC 6 cut(s) 184, 403, 450, 653, 1099, 1746
PleI GAGTC 3 cut(s) 547, 1308, 2013
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 3 cut(s) 547, 1308, 2013
Ppu21I YACGTR 2 cut(s) 503, 1800
PsiI TTATAA 1 cut(s) 1632
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 6 cut(s) 216, 390, 562, 662, 1116, 1481
PspCI CACGTG 1 cut(s) 503
PspGI CCWGG 6 cut(s) 216, 390, 562, 662, 1116, 1481
PspN4I GGNNCC 4 cut(s) 291, 568, 687, 1039
PspPI GGNCC 4 cut(s) 317, 566, 685, 1841
PstI CTGCAG 2 cut(s) 2041, 2051
PsuI RGATCY 3 cut(s) 766, 1366, 1996
PsyI GACNNNGTC 2 cut(s) 1711, 2063
RsaI GTAC 5 cut(s) 66, 459, 1151, 1241, 1721
RsaNI GTAC 5 cut(s) 65, 458, 1150, 1240, 1720
RseI CAYNNNNRTG 2 cut(s) 312, 890
SacI GAGCTC 1 cut(s) 861
SapI GCTCTTC 1 cut(s) 1388
SaqAI TTAA 9 cut(s) 95, 203, 264, 534, 549, 741, 1362, 1641, 1656
SatI GCNGC 6 cut(s) 183, 402, 449, 652, 1098, 1745
Sau96I GGNCC 4 cut(s) 317, 566, 685, 1841
SchI GAGTC 3 cut(s) 548, 1309, 2014
ScrFI CCNGG 7 cut(s) 56, 218, 392, 564, 664, 1118, 1483
SduI GDGCHC 4 cut(s) 109, 292, 508, 861
SfaNI GCATC 3 cut(s) 601, 823, 1936
SfcI CTRYAG 5 cut(s) 1517, 1542, 1764, 2037, 2047
Sfr274I CTCGAG 1 cut(s) 1880
SfuI TTCGAA 3 cut(s) 115, 602, 1231
SinI GGWCC 4 cut(s) 317, 566, 685, 1841
SlaI CTCGAG 1 cut(s) 1880
SmiMI CAYNNNNRTG 2 cut(s) 312, 890
SmlI CTYRAG 6 cut(s) 344, 1160, 1280, 1361, 1406, 1880
SmoI CTYRAG 6 cut(s) 344, 1160, 1280, 1361, 1406, 1880
SphI GCATGC 1 cut(s) 2165
SsiI CCGC 2 cut(s) 404, 1792
SspI AATATT 3 cut(s) 709, 1138, 1309
SspMI CTAG 4 cut(s) 129, 1838, 1958, 2027
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 7 cut(s) 54, 216, 390, 562, 662, 1116, 1481
StyI CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
TaaI ACNGT 4 cut(s) 457, 1093, 1605, 1765
TaiI ACGT 2 cut(s) 505, 1802
TaqI TCGA 7 cut(s) 115, 602, 1231, 1298, 1372, 1695, 1881
TatI WGTACW 2 cut(s) 64, 1719
TfiI GAWTC 8 cut(s) 49, 477, 659, 1052, 1121, 1244, 1513, 1788
Tru1I TTAA 9 cut(s) 95, 203, 264, 534, 549, 741, 1362, 1641, 1656
Tru9I TTAA 9 cut(s) 95, 203, 264, 534, 549, 741, 1362, 1641, 1656
TscAI CASTG 4 cut(s) 28, 720, 949, 2051
TseFI GTSAC 4 cut(s) 1505, 1526, 2042, 2095
TseI GCWGC 6 cut(s) 182, 401, 448, 651, 1097, 1744
Tsp45I GTSAC 4 cut(s) 1505, 1526, 2042, 2095
TspDTI ATGAA 7 cut(s) 406, 856, 888, 1057, 1315, 1417, 1650
TspRI CASTG 4 cut(s) 28, 720, 949, 2051
Tth111I GACNNNGTC 2 cut(s) 1711, 2063
Vha464I CTTAAG 1 cut(s) 1361
VneI GTGCAC 1 cut(s) 105
VpaK11BI GGWCC 4 cut(s) 317, 566, 685, 1841
XapI RAATTY 2 cut(s) 1424, 1875
XceI RCATGY 3 cut(s) 1869, 1953, 2165
XhoI CTCGAG 1 cut(s) 1880
XmiI GTMKAC 2 cut(s) 1728, 1760
XmnI GAANNNNTTC 2 cut(s) 1492, 1817
XspI CTAG 4 cut(s) 129, 1838, 1958, 2027
Zsp2I ATGCAT 1 cut(s) 1951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.