AT5G45480

Protein of unknown function, DUF594

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000662
Physical Location & Seq
Reverse (-)
18426104 .. 18429090
2987 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45480.1

Sequence Viewer

Length: 2634 bp
ATGGTGAACGCAATCCCAAAGCCTATCAAGGACATTTGGGACGAATGGAGCATTCGAAGTACCTTAATCTTCAGTCTCAGCCTTCAAACATTTCTCATTTTCTTTGCACCTCAAAGAAAACGCTCATCCAGAAAAGTTTTACTCTCTTTCATTTGGTCAGCTTACCTTCTTGCTGATTGGTCTGCCAATTTCGCCGCCGGACAAATCTCAGATAGTCAAGGAGATGATCCAGAACCCGGAGAACCGAAAAAGAGCGCCGAGTTGTTTGCTTTCTGGGTTCCTTTCTTGCTCTTGCATCTCGGTGGTCCAGATACAATCACTGCCCTTGCCCTTGAAGATAACGAGCTCTGGCTCAGGCACTTGTTGGGTCTTTTCTTTCAATCAGTTGCAACCGTTTACGTGCTTTTGCAGTCGTTACCCAATGCTCTTTGGAAACCTATTTTGCTGGTGTTTGCGACCGGGGTGATCAAGTATGTCGAGAGGACATTAGCTTTGTACCTGGCGAGTCTTGACAAGTTCAAAGATTCCATGATTCAGCGGCCTGATCCTGGACCAAACTACGCAAAGCTCATGGAGGAATACGCAGCCAAGAAAGATATGAAGATGCCTACACAGATTATCAAGGTTGGCGAGCCTGAGAAAGATCCAAGAGACGATGCTCCGGTAAAGCCTCCTGACGGGTTCACTCCACTCAATATTCTCCAGTACGCCTACAAGTACTTTAATATCTTCAAAGGTCTTGTGGTTGATCTCATCTTTACATTTCAACAACGAGCTGAGAGCAAGAGATTCTTTGACTCGCTGAAAGCAGAAGAAGCTTTAAGAATCTTAGAGGTTGAGCTCAACTTTATCTACGCAGCTCTATACACGAAAGCCGAGATCTTGCACAACTGGATAGGATTCCTCTTCAGGTTCATTGCCCTGGGATGCCTTGCAGCAGCTCTACGCATCTTTCAATACAAGAGCAAGAAAGATTATAGTGGATTTGATGTTGGCCTTACTTACGCATTGCTGTTAGGAGGAATAGCTCTGGATTGCATCGCTCTTATCATGTTCTGTGCATCTGATTGGACGTTTGTCAGATTGAGGAAGATGAAGGACGAAGTGGACGATCCACCCACCTGGTCCGACAACATTCTTAACTGGATTCTTGAAAACATTCTCGGGGTCAGGAAATTGAAGGTGGAGGAGTATGATGAATGCTACAAGAATACACAGTCTCATGAAGTGCCCAACACGAGTACTAAGAAAACACCATTTCTTAAAAGGATTCTTAACAGGATTCTCAGGGTGAGGGAGTTGAAGACAGAGAAGTCTCATGAAGTTCTCGACAAGAGTACCAGCAAAATACCAGGTCAGGAAGTGCCCGACAAGAGTGCCAAGACAATACCATGTCATAAAGTGCTCGACACGAGTTTCATGTATCGCCGGTGGTCTGAGTATGTACATGCACACAACCTGATCGAATACTGCCTAGGTTTAAAACCGAAGCGAATCCACCATACCAAGGGTTTCATACACATCGCCTTTGACAAGGTTATCAACATACTTTACATTGGCCCCGCATTCACAAAACTTGGAAGTGTCATTGAATCCTGTTTTAGAGTCACAAAACAGAGAATTCATCAGACTTTCAAGTGGATTGACGGTAAAGTAAGTCGTTTATGCAAGAAATACCCGAAGTTGAATGAAGAGTACATTAGGTTTAGCTTCTTCTGCATATTTTACATTCCTAGTCTACCTGGACGCTGGATAAAAAGTTTTATGGAGTTCTTTGGCATCCGAGCTCAGCTAGATGAGGTGATCTACACATCTAGTGATCGTCTCACCCTGGATATGTGGGAACACATATTCGGAGAGGTTAAAGCCAAATCCCGTTTCGCTGACGACTCTGAAAGCGCCATGAGGGTATCTTCAGCAAGAGGTGACTGGACACTGCGCGACATACAAGGTGACCCAGAAACAGAAAAAAAACGTGAGAAACTTCTACGCTATGTGATGGAAATGGATTACGATCAGAGCCTTCTTGTATGGCATATAGCCACTGAGCTCTTATACCAGACAAAAAAAGGCACTAAAGCGAATCATAGCGCCCGTGAGTTCAGTAAAATCCTGTCAGACTACATGATGTATCTCCTAATGATGCAACCCACTCTGATGTCAGCTGTTGTGGGTATTGGAAAGATAAGATTCAGAGACACGTGCGAGGAGGCTCAGAGGTTCTTCGATAGGAGGCATATCATGGGGATATCCGCAAAGAAGGCCCCTGATGCAAAGGAAGCCAGCGTGGCAATTTTATCAGTGGCAGTTCCAGCAAAAGCTGAGCCCATTGACGTGAAAGGAGATAGAAGCAAATCCGTCCTGTTTGATGGGGCCATGCTGGCGAAAGAGCTTAAGGGTTTAAGAAAGAATAAGGAGGACGACTCAGAGATGTGGAAAATAATGAGCCAAGTGTGGGTTGAGTTGCTTTCGTATGCAGCGACAAAGTGTGGCGCTATAGAACACGCAGCTCAGCTAAGCAAAGGAGGAGAACTCATAAGCTTTGTTTGGCTATTGATGGCTCATTTTGGACTAGGAGACCAATTCCAAATCAACCAAGGAGATGCCAGAGCCAAACTGATTATAGGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

877

Amino Acids

100.8

Weight (kDa)

8.99

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 489 1e-96 Domain of unknown function (DUF4220)
DUF594 PF04578 805 - 858 2e-24 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1312
AccI GTMKAC 1 cut(s) 1738
AccII CGCG 1 cut(s) 1941
AciI CCGC 4 cut(s) 195, 538, 1563, 2253
AclWI GGATC 4 cut(s) 221, 539, 638, 1106
AcsI RAATTY 1 cut(s) 1620
AcuI CTGAAG 3 cut(s) 55, 892, 1899
AcvI CACGTG 1 cut(s) 2202
AfaI GTAC 8 cut(s) 61, 497, 707, 719, 1243, 1339, 1446, 1697
AfiI CCNNNNNNNGG 5 cut(s) 236, 548, 677, 1507, 2455
AflII CTTAAG 1 cut(s) 2393
AflIII ACRYGT 1 cut(s) 2199
AjiI CACGTC 1 cut(s) 2335
AjnI CCWGG 7 cut(s) 498, 547, 921, 1121, 1351, 1741, 1830
AjuI GAANNNNNNNTTGG 2 cut(s) 1862, 1894
Alw21I GWGCWC 5 cut(s) 348, 843, 1407, 1789, 2052
Alw26I GTCTC 7 cut(s) 80, 645, 1224, 1320, 1829, 2190, 2571
AlwI GGATC 4 cut(s) 221, 539, 638, 1106
Ama87I CYCGRG 1 cut(s) 1163
AoxI GGCC 5 cut(s) 539, 994, 1558, 2262, 2373
ApeKI GCWGC 6 cut(s) 584, 857, 935, 938, 2477, 2507
ApoI RAATTY 1 cut(s) 1620
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
Asp700I GAANNNNTTC 1 cut(s) 1158
AspA2I CCTAGG 1 cut(s) 1474
AspLEI GCGC 5 cut(s) 257, 1901, 1941, 2093, 2495
AspS9I GGNCC 6 cut(s) 305, 551, 1125, 1559, 2263, 2373
AsuC2I CCSGG 2 cut(s) 237, 460
AsuHPI GGTGA 7 cut(s) 16, 475, 1303, 1813, 1819, 1937, 1964
AsuII TTCGAA 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 1163
AvaII GGWCC 3 cut(s) 305, 551, 1125
AvrII CCTAGG 1 cut(s) 1474
BaeGI GKGCMC 2 cut(s) 1233, 1368
BaeI ACNNNNGTAYC 2 cut(s) 1321, 1354
BanII GRGCYC 5 cut(s) 348, 843, 1789, 2052, 2328
BauI CACGAG 2 cut(s) 1237, 1411
BbrPI CACGTG 1 cut(s) 2202
BbsI GAAGAC 1 cut(s) 1310
Bbv12I GWGCWC 5 cut(s) 348, 843, 1407, 1789, 2052
BbvI GCAGC 6 cut(s) 596, 869, 947, 950, 2489, 2519
BccI CCATC 3 cut(s) 1993, 2363, 2551
BcgI CGANNNNNNTGC 4 cut(s) 248, 282, 1358, 1392
BciT130I CCWGG 7 cut(s) 500, 549, 923, 1123, 1353, 1743, 1832
BclI TGATCA 1 cut(s) 465
BcnI CCSGG 2 cut(s) 237, 460
BcoDI GTCTC 7 cut(s) 80, 645, 1224, 1320, 1829, 2190, 2571
BfaI CTAG 5 cut(s) 1475, 1734, 1793, 1815, 2573
BfmI CTRYAG 1 cut(s) 2496
BfoI RGCGCY 4 cut(s) 258, 1902, 2094, 2496
BfrI CTTAAG 1 cut(s) 2393
BglI GCCNNNNNGGC 2 cut(s) 2288, 2381
BglII AGATCT 1 cut(s) 879
BisI GCNGC 8 cut(s) 195, 539, 585, 858, 936, 939, 2478, 2508
BlnI CCTAGG 1 cut(s) 1474
BlpI GCTNAGC 4 cut(s) 1788, 2322, 2511, 2516
BlsI GCNGC 8 cut(s) 196, 540, 586, 859, 937, 940, 2479, 2509
BmcAI AGTACT 2 cut(s) 719, 1243
Bme1390I CCNGG 9 cut(s) 237, 460, 500, 549, 923, 1123, 1353, 1743, 1832
Bme18I GGWCC 3 cut(s) 305, 551, 1125
BmeT110I CYCGRG 1 cut(s) 1163
BmgBI CACGTC 1 cut(s) 2335
BmgT120I GGNCC 6 cut(s) 305, 551, 1125, 1559, 2263, 2373
BmiI GGNNCC 4 cut(s) 279, 1561, 2265, 2374
BmrFI CCNGG 9 cut(s) 237, 460, 500, 549, 923, 1123, 1353, 1743, 1832
BoxI GACNNNNGTC 1 cut(s) 1076
BpiI GAAGAC 1 cut(s) 1310
BplI GAGNNNNNCTC 4 cut(s) 2408, 2440, 2517, 2549
BpmI CTGGAG 1 cut(s) 686
Bpu10I CCTNAGC 1 cut(s) 353
Bpu1102I GCTNAGC 4 cut(s) 1788, 2322, 2511, 2516
Bpu14I TTCGAA 1 cut(s) 55
BpuMI CCSGG 2 cut(s) 237, 460
BsaAI YACGTR 2 cut(s) 400, 2202
BsaBI GATNNNNATC 1 cut(s) 2013
BsaI GGTCTC 1 cut(s) 2571
BsaJI CCNNGG 7 cut(s) 459, 921, 922, 1474, 1506, 1830, 2596
BsaWI WCCGGW 1 cut(s) 661
Bsc4I CCNNNNNNNGG 5 cut(s) 236, 548, 677, 1507, 2455
Bse118I RCCGGY 1 cut(s) 1428
Bse1I ACTGG 4 cut(s) 703, 896, 1148, 1934
Bse3DI GCAATG 2 cut(s) 915, 1007
Bse8I GATNNNNATC 1 cut(s) 2013
BseBI CCWGG 7 cut(s) 500, 549, 923, 1123, 1353, 1743, 1832
BseDI CCNNGG 7 cut(s) 459, 921, 922, 1474, 1506, 1830, 2596
BseGI GGATG 3 cut(s) 125, 932, 1779
BseJI GATNNNNATC 1 cut(s) 2013
BseLI CCNNNNNNNGG 5 cut(s) 236, 548, 677, 1507, 2455
BseMI GCAATG 2 cut(s) 915, 1007
BseNI ACTGG 4 cut(s) 703, 896, 1148, 1934
BseRI GAGGAG 3 cut(s) 1202, 2222, 2541
BseSI GKGCMC 2 cut(s) 1233, 1368
BseXI GCAGC 6 cut(s) 596, 869, 947, 950, 2489, 2519
Bsh1236I CGCG 1 cut(s) 1941
Bsh1285I CGRYCG 1 cut(s) 459
BshFI GGCC 5 cut(s) 541, 996, 1560, 2264, 2375
BsiEI CGRYCG 1 cut(s) 459
BsiHKAI GWGCWC 5 cut(s) 348, 843, 1407, 1789, 2052
BsiHKCI CYCGRG 1 cut(s) 1163
BsiSI CCGG 5 cut(s) 198, 237, 459, 662, 1429
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 5 cut(s) 236, 548, 677, 1507, 2455
BsmAI GTCTC 7 cut(s) 80, 645, 1224, 1320, 1829, 2190, 2571
BsmBI CGTCTC 2 cut(s) 645, 1829
BsmFI GGGAC 1 cut(s) 53
BsmI GAATGC 3 cut(s) 51, 1205, 1565
BsnI GGCC 5 cut(s) 541, 996, 1560, 2264, 2375
Bso31I GGTCTC 1 cut(s) 2571
BsoBI CYCGRG 1 cut(s) 1163
Bsp119I TTCGAA 1 cut(s) 55
Bsp1286I GDGCHC 8 cut(s) 348, 843, 1233, 1368, 1407, 1789, 2052, 2328
Bsp1407I TGTACA 1 cut(s) 1444
Bsp1720I GCTNAGC 4 cut(s) 1788, 2322, 2511, 2516
BspACI CCGC 4 cut(s) 195, 538, 1563, 2253
BspANI GGCC 5 cut(s) 541, 996, 1560, 2264, 2375
BspFNI CGCG 1 cut(s) 1941
BspHI TCATGA 2 cut(s) 1222, 1318
BspLI GGNNCC 4 cut(s) 279, 1561, 2265, 2374
BspPI GGATC 4 cut(s) 221, 539, 638, 1106
BspT104I TTCGAA 1 cut(s) 55
BspTI CTTAAG 1 cut(s) 2393
BspTNI GGTCTC 1 cut(s) 2571
BsrDI GCAATG 2 cut(s) 915, 1007
BsrFI RCCGGY 1 cut(s) 1428
BsrGI TGTACA 1 cut(s) 1444
BsrI ACTGG 4 cut(s) 703, 896, 1148, 1934
BssAI RCCGGY 1 cut(s) 1428
BssECI CCNNGG 7 cut(s) 459, 921, 922, 1474, 1506, 1830, 2596
BssSI CACGAG 2 cut(s) 1237, 1411
BssT1I CCWWGG 3 cut(s) 1474, 1506, 2596
Bst2BI CACGAG 2 cut(s) 1237, 1411
Bst2UI CCWGG 7 cut(s) 500, 549, 923, 1123, 1353, 1743, 1832
Bst4CI ACNGT 3 cut(s) 394, 1218, 1649
Bst6I CTCTTC 2 cut(s) 911, 1686
BstAFI CTTAAG 1 cut(s) 2393
BstAUI TGTACA 1 cut(s) 1444
BstBAI YACGTR 2 cut(s) 400, 2202
BstBI TTCGAA 1 cut(s) 55
BstC8I GCNNGC 3 cut(s) 632, 2284, 2382
BstEII GGTNACC 1 cut(s) 1952
BstF5I GGATG 3 cut(s) 125, 932, 1779
BstFNI CGCG 1 cut(s) 1941
BstH2I RGCGCY 4 cut(s) 258, 1902, 2094, 2496
BstHHI GCGC 5 cut(s) 257, 1901, 1941, 2093, 2495
BstMAI GTCTC 7 cut(s) 80, 645, 1224, 1320, 1829, 2190, 2571
BstMCI CGRYCG 1 cut(s) 459
BstMWI GCNNNNNNNGC 9 cut(s) 191, 815, 1716, 2261, 2270, 2279, 2288, 2312, 2381
BstNI CCWGG 7 cut(s) 500, 549, 923, 1123, 1353, 1743, 1832
BstNSI RCATGY 1 cut(s) 1451
BstPAI GACNNNNGTC 1 cut(s) 1076
BstPI GGTNACC 1 cut(s) 1952
BstSCI CCNGG 9 cut(s) 235, 458, 498, 547, 921, 1121, 1351, 1741, 1830
BstSFI CTRYAG 1 cut(s) 2496
BstSLI GKGCMC 2 cut(s) 1233, 1368
BstUI CGCG 1 cut(s) 1941
BstV1I GCAGC 6 cut(s) 596, 869, 947, 950, 2489, 2519
BstV2I GAAGAC 1 cut(s) 1310
BstX2I RGATCY 2 cut(s) 643, 879
BstXI CCANNNNNNTGG 1 cut(s) 1122
BstYI RGATCY 2 cut(s) 643, 879
BsuRI GGCC 5 cut(s) 541, 996, 1560, 2264, 2375
BtgZI GCGATG 2 cut(s) 1024, 1507
BtrI CACGTC 1 cut(s) 2335
BtsCI GGATG 3 cut(s) 125, 932, 1779
BtsI GCAGTG 2 cut(s) 318, 1934
BtsIMutI CAGTG 4 cut(s) 318, 1934, 2043, 2307
Cac8I GCNNGC 3 cut(s) 632, 2284, 2382
CciI TCATGA 2 cut(s) 1222, 1318
CfoI GCGC 5 cut(s) 257, 1901, 1941, 2093, 2495
Cfr10I RCCGGY 1 cut(s) 1428
Cfr13I GGNCC 6 cut(s) 305, 551, 1125, 1559, 2263, 2373
CseI GACGC 1 cut(s) 1755
CsiI ACCWGGT 2 cut(s) 1121, 1351
Csp6I GTAC 8 cut(s) 60, 496, 706, 718, 1242, 1338, 1445, 1696
CviQI GTAC 8 cut(s) 60, 496, 706, 718, 1242, 1338, 1445, 1696
DraI TTTAAA 1 cut(s) 1482
DrdI GACNNNNNNGTC 1 cut(s) 1312
DseDI GACNNNNNNGTC 1 cut(s) 1312
Eam1104I CTCTTC 2 cut(s) 911, 1686
EarI CTCTTC 2 cut(s) 911, 1686
Ecl136II GAGCTC 4 cut(s) 346, 841, 1787, 2050
Eco130I CCWWGG 3 cut(s) 1474, 1506, 2596
Eco24I GRGCYC 5 cut(s) 348, 843, 1789, 2052, 2328
Eco31I GGTCTC 1 cut(s) 2571
Eco32I GATATC 1 cut(s) 2250
Eco47I GGWCC 3 cut(s) 305, 551, 1125
Eco53kI GAGCTC 4 cut(s) 346, 841, 1787, 2050
Eco57I CTGAAG 3 cut(s) 55, 892, 1899
Eco72I CACGTG 1 cut(s) 2202
Eco88I CYCGRG 1 cut(s) 1163
Eco91I GGTNACC 1 cut(s) 1952
EcoICRI GAGCTC 4 cut(s) 346, 841, 1787, 2050
EcoO109I RGGNCCY 1 cut(s) 2263
EcoO65I GGTNACC 1 cut(s) 1952
EcoRI GAATTC 1 cut(s) 1620
EcoRII CCWGG 7 cut(s) 498, 547, 921, 1121, 1351, 1741, 1830
EcoRV GATATC 1 cut(s) 2250
EcoT14I CCWWGG 3 cut(s) 1474, 1506, 2596
EcoT38I GRGCYC 5 cut(s) 348, 843, 1789, 2052, 2328
ErhI CCWWGG 3 cut(s) 1474, 1506, 2596
Esp3I CGTCTC 2 cut(s) 645, 1829
FalI AAGNNNNNCTT 2 cut(s) 776, 808
FaqI GGGAC 1 cut(s) 53
FauI CCCGC 1 cut(s) 1570
FbaI TGATCA 1 cut(s) 465
FblI GTMKAC 1 cut(s) 1738
Fnu4HI GCNGC 8 cut(s) 195, 539, 585, 858, 936, 939, 2478, 2508
FokI GGATG 3 cut(s) 112, 939, 1766
FriOI GRGCYC 5 cut(s) 348, 843, 1789, 2052, 2328
Fsp4HI GCNGC 8 cut(s) 195, 539, 585, 858, 936, 939, 2478, 2508
FspBI CTAG 5 cut(s) 1475, 1734, 1793, 1815, 2573
GlaI GCGC 5 cut(s) 256, 1900, 1940, 2092, 2494
GluI GCNGC 8 cut(s) 195, 539, 585, 858, 936, 939, 2478, 2508
GsuI CTGGAG 1 cut(s) 686
HaeII RGCGCY 4 cut(s) 258, 1902, 2094, 2496
HaeIII GGCC 5 cut(s) 541, 996, 1560, 2264, 2375
HapII CCGG 5 cut(s) 198, 237, 459, 662, 1429
HgaI GACGC 1 cut(s) 1755
HhaI GCGC 5 cut(s) 257, 1901, 1941, 2093, 2495
Hin6I GCGC 5 cut(s) 255, 1899, 1939, 2091, 2493
HinP1I GCGC 5 cut(s) 255, 1899, 1939, 2091, 2493
HindIII AAGCTT 2 cut(s) 816, 2539
HpaII CCGG 5 cut(s) 198, 237, 459, 662, 1429
HphI GGTGA 7 cut(s) 16, 475, 1303, 1813, 1819, 1937, 1964
Hpy166II GTNNAC 5 cut(s) 7, 397, 684, 1108, 1739
Hpy8I GTNNAC 5 cut(s) 7, 397, 684, 1108, 1739
HpyAV CCTTC 6 cut(s) 92, 176, 1090, 1174, 2033, 2254
HpyCH4III ACNGT 3 cut(s) 394, 1218, 1649
HpyCH4IV ACGT 5 cut(s) 399, 1073, 1975, 2201, 2334
HpyF10VI GCNNNNNNNGC 9 cut(s) 191, 815, 1716, 2261, 2270, 2279, 2288, 2312, 2381
HpySE526I ACGT 5 cut(s) 399, 1073, 1975, 2201, 2334
HspAI GCGC 5 cut(s) 255, 1899, 1939, 2091, 2493
Ksp22I TGATCA 1 cut(s) 465
LmnI GCTCC 2 cut(s) 48, 664
Lsp1109I GCAGC 6 cut(s) 596, 869, 947, 950, 2489, 2519
MabI ACCWGGT 2 cut(s) 1121, 1351
MaeI CTAG 5 cut(s) 1475, 1734, 1793, 1815, 2573
MaeII ACGT 5 cut(s) 399, 1073, 1975, 2201, 2334
MaeIII GTNAC 4 cut(s) 414, 1606, 1925, 1952
MflI RGATCY 2 cut(s) 643, 879
MhlI GDGCHC 8 cut(s) 348, 843, 1233, 1368, 1407, 1789, 2052, 2328
MluCI AATT 5 cut(s) 187, 1175, 1620, 2292, 2582
MlyI GAGTC 5 cut(s) 514, 791, 1614, 1883, 2417
MmeI TCCRAC 1 cut(s) 1152
MroXI GAANNNNTTC 1 cut(s) 1158
MslI CAYNNNNRTG 3 cut(s) 300, 2156, 2333
MspA1I CMGCKG 2 cut(s) 538, 2165
MspCI CTTAAG 1 cut(s) 2393
MspI CCGG 5 cut(s) 198, 237, 459, 662, 1429
MspR9I CCNGG 9 cut(s) 237, 460, 500, 549, 923, 1123, 1353, 1743, 1832
Mva1269I GAATGC 3 cut(s) 51, 1205, 1565
MvaI CCWGG 7 cut(s) 500, 549, 923, 1123, 1353, 1743, 1832
MvnI CGCG 1 cut(s) 1941
MwoI GCNNNNNNNGC 9 cut(s) 191, 815, 1716, 2261, 2270, 2279, 2288, 2312, 2381
NciI CCSGG 2 cut(s) 237, 460
NlaIV GGNNCC 4 cut(s) 279, 1561, 2265, 2374
NmeAIII GCCGAG 2 cut(s) 283, 901
NmuCI GTSAC 3 cut(s) 1606, 1925, 1952
NspI RCATGY 1 cut(s) 1451
NspV TTCGAA 1 cut(s) 55
PagI TCATGA 2 cut(s) 1222, 1318
PasI CCCWGGG 1 cut(s) 922
PcsI WCGNNNNNNNCGW 2 cut(s) 1107, 2477
PctI GAATGC 3 cut(s) 51, 1205, 1565
PdmI GAANNNNTTC 1 cut(s) 1158
PfoI TCCNGGA 1 cut(s) 547
PkrI GCNGC 8 cut(s) 196, 540, 586, 859, 937, 940, 2479, 2509
PleI GAGTC 5 cut(s) 513, 791, 1613, 1883, 2417
PmaCI CACGTG 1 cut(s) 2202
PmlI CACGTG 1 cut(s) 2202
PpsI GAGTC 5 cut(s) 513, 791, 1613, 1883, 2417
Ppu21I YACGTR 2 cut(s) 400, 2202
PshAI GACNNNNGTC 1 cut(s) 1076
Psp124BI GAGCTC 4 cut(s) 348, 843, 1789, 2052
Psp6I CCWGG 7 cut(s) 498, 547, 921, 1121, 1351, 1741, 1830
PspCI CACGTG 1 cut(s) 2202
PspEI GGTNACC 1 cut(s) 1952
PspGI CCWGG 7 cut(s) 498, 547, 921, 1121, 1351, 1741, 1830
PspN4I GGNNCC 4 cut(s) 279, 1561, 2265, 2374
PspPI GGNCC 6 cut(s) 305, 551, 1125, 1559, 2263, 2373
PsuI RGATCY 2 cut(s) 643, 879
PvuII CAGCTG 1 cut(s) 2165
RsaI GTAC 8 cut(s) 61, 497, 707, 719, 1243, 1339, 1446, 1697
RsaNI GTAC 8 cut(s) 60, 496, 706, 718, 1242, 1338, 1445, 1696
RseI CAYNNNNRTG 3 cut(s) 300, 2156, 2333
SacI GAGCTC 4 cut(s) 348, 843, 1789, 2052
SatI GCNGC 8 cut(s) 195, 539, 585, 858, 936, 939, 2478, 2508
Sau96I GGNCC 6 cut(s) 305, 551, 1125, 1559, 2263, 2373
ScaI AGTACT 2 cut(s) 719, 1243
SchI GAGTC 5 cut(s) 514, 791, 1614, 1883, 2417
ScrFI CCNGG 9 cut(s) 237, 460, 500, 549, 923, 1123, 1353, 1743, 1832
SduI GDGCHC 8 cut(s) 348, 843, 1233, 1368, 1407, 1789, 2052, 2328
SexAI ACCWGGT 2 cut(s) 1121, 1351
SfcI CTRYAG 1 cut(s) 2496
SfuI TTCGAA 1 cut(s) 55
SgrAI CRCCGGYG 1 cut(s) 1428
SinI GGWCC 3 cut(s) 305, 551, 1125
SmiMI CAYNNNNRTG 3 cut(s) 300, 2156, 2333
SmlI CTYRAG 1 cut(s) 2393
SmoI CTYRAG 1 cut(s) 2393
Sse9I AATT 5 cut(s) 187, 1175, 1620, 2292, 2582
SsiI CCGC 4 cut(s) 195, 538, 1563, 2253
SspI AATATT 1 cut(s) 697
SspMI CTAG 5 cut(s) 1475, 1734, 1793, 1815, 2573
SstI GAGCTC 4 cut(s) 348, 843, 1789, 2052
StyD4I CCNGG 9 cut(s) 235, 458, 498, 547, 921, 1121, 1351, 1741, 1830
StyI CCWWGG 3 cut(s) 1474, 1506, 2596
TaaI ACNGT 3 cut(s) 394, 1218, 1649
TaiI ACGT 5 cut(s) 402, 1076, 1978, 2204, 2337
TaqI TCGA 6 cut(s) 55, 477, 1329, 1407, 1464, 2226
TasI AATT 5 cut(s) 187, 1175, 1620, 2292, 2582
TatI WGTACW 4 cut(s) 717, 1241, 1444, 1695
TauI GCSGC 2 cut(s) 197, 541
TscAI CASTG 4 cut(s) 325, 1941, 2050, 2307
TseFI GTSAC 3 cut(s) 1606, 1925, 1952
TseI GCWGC 6 cut(s) 584, 857, 935, 938, 2477, 2507
Tsp45I GTSAC 3 cut(s) 1606, 1925, 1952
TspGWI ACGGA 1 cut(s) 2347
TspRI CASTG 4 cut(s) 325, 1941, 2050, 2307
Vha464I CTTAAG 1 cut(s) 2393
VpaK11BI GGWCC 3 cut(s) 305, 551, 1125
XapI RAATTY 1 cut(s) 1620
XceI RCATGY 1 cut(s) 1451
XmaJI CCTAGG 1 cut(s) 1474
XmiI GTMKAC 1 cut(s) 1738
XmnI GAANNNNTTC 1 cut(s) 1158
XspI CTAG 5 cut(s) 1475, 1734, 1793, 1815, 2573
ZrmI AGTACT 2 cut(s) 719, 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.