RchiOBHm_Chr2g0112871

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000662
Physical Location & Seq
Reverse (-)
24951675 .. 24953942
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48628

Sequence Viewer

Length: 2268 bp
ATGGCTTCCTCAGCGGCTCCTATTTCTGACAGCCTCAAGAAGATCTGGGATCAGTGGAATCTCCGGAGCTTCATCATGTTAAGTCTCACATTGCAGACCTTCTTAATTCTCTTCGCACCCTTGAGAAAGCGATCATCGAGCAAATGGGTAATCGTGCCCATCTGGTTGGCTTACTTGCTTGCTGACTGGGCTGCTAACTTCGCTATCGGATTAATTGCAAGCAGCCAAACGAACACTGATCAAGGCTCGCCAGAGAATGGTAATCTATTGGCATTCTGGGCTCCCTTTCTTCTTTTGCACCTTGGTGGTCCAGACACCATAATTGCAATTGCTATGGAAGACAATGCGTTGTGGCCAAGGCACTTGCTTGGTCTCATATTCCAGGTTGTAGCTGCAATGTACGTCTTCGGTATGCAGTTCTCTAACCAAAACAAACTACTGTGGCCAACACTCTTCCTGTTTGTACCAGGACTCGTCAAGTACGGCGAGCGAACACGTGCTTTATATCTTGCCAGCTTGGAAAGTCTCAAGGAAACCATACTTGCAGTACCGGAACCTGGACCGGATTATGCAAAGTACATGGATGAGTACTCCTCAAGAAAAGCTGCCCATCTTCCAATTAAAATAGAAATCAAAGAAGGTCGTAGCTCAGATTCGAAGAATGTGACGTATCGGCTGGGAGAAGGAGACGTCATTGATAATATTACACTAGTGGAGGGTGCTCATCACTTGTTTCAAATTTCCATGGGTCTTATTATAGATCTTGTGTTCGACTATCATCAGCGCAAAGAGAGCCGTGCCTATTTCATCAAATTAGAATCAGGAGATGCTTTTACATCAATTGCTACTGAGCTCAATTTTATGTATGAAGCTCTGTACACCAAAGTTTATGTGGTACATTCTCGACGGGGATACTTGTTGAGAGCTACATCCTTCATCGCCGTTTGCATAGCCTTTGTTTTCTTCTATAGATTAGAGAAACATGGCTTCCACAGCTTTGATGTTGGAATTACTTATTCCTTGCTTTTTGGTGCCGTAGCTCTTGATTCAATAGCTCTTTCGATGCTGATATTATCTGATTGGACGATCGCTGTTGTATACAAGTACTGTAAAAAGTGTTGTGTAGTCACTGATCTCATGATGAAGTACCTGGAGTTTAAGAGATCATGGCGACCAACTACTCCACCAAAGTGTCTGGAGCGGACCAGAAAGATTTTATTTCGAAGATGGTGTGAATCTGTCTCCGGTTTCAACTTCATAGATTATTCTCTCCGAGAATGTGGAAGATCAGTAGTCCGACCTGTGTACCGTTTTGGCATTTTTGAAATTTACCGTACAGTCATCAGATTTTTGGGCTACCTTTACCATAGAATCATCATATTCCTGGGCCACCTTTACCTTAGAATCATTAAATTCTTTGGCCTCAAGTATCTTCGAGATGAGATGAAGTATAGATCCAGCAAGAGACTGGATGAGAATTTGTGGAAGTTCATTTTTGATGAGTTGAAAAGTAAATCCTCAGAAGTTGCTGATGATCCCAAAATTGGTAGAGAAATATGTTCGTCCCAAGGGGATTGGGTTCTCCTAAAAAAGTATGAGAAGCATCCGGAGTACAAGAACTTGCTAGCATGCTTTGTTGATGTTACTTATGACCGACGCATTCTGATGTGGCATATTGCTACTGAGATGTGCTATTACAGAGTTGACACAGGCAATGGTGGTGAGCATGGTGAATATAGTAAGACGTTGTCTGATTATTTGCTATATCTCTTAATTATGCAACCTGCTTTGATGTCCTCTGTAGCGGGAATTGGACAAAAGAGATTTAGAGATACGGTTGCAGAAGCCAAAAAATTCTTTAAAAAAGGGCGAGTGGGATCCAATGTGAATCTAGGCGAGGGAAAACTAGGCGAGGCTTGTTACAAACTCCTTGATGTAGATACAGGAGTTAAACCTGTCAATGTCAAAGGGAATAGAAGTAAATCTGTTTTGTTTGATGCATGTATTCTGGCCAAGCAGCTGATGAAATTCGAAGACTCGAAATGGGAATTAATAAGCTCGGTGTGGGTGGAATTGCTATCATATGCTGCCAGTCATTGCAGAGCACAAAGCCACGCGCAATTGCTGACTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTACTGATGGCTCGCTTTGGCATTGAGGAGGACATCCAAGTACTGGAGGTCCAAGCAAGAGTAAAACTGATCATGGAAAAGCAACCTGATGTATTCCAGAGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

755

Amino Acids

86.81

Weight (kDa)

9.05

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 422 3.6e-91 Domain of unknown function (DUF4220)
DUF594 PF04578 678 - 728 7.6e-22 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 693
Acc36I ACCTGC 1 cut(s) 1792
AccB1I GGYRCC 1 cut(s) 1031
AccB7I CCANNNNNTGG 2 cut(s) 257, 2203
AccBSI CCGCTC 1 cut(s) 1201
AccI GTMKAC 1 cut(s) 1098
AccII CGCG 1 cut(s) 2117
AccIII TCCGGA 2 cut(s) 63, 1606
AciI CCGC 3 cut(s) 14, 1201, 1805
AclWI GGATC 5 cut(s) 57, 1449, 1529, 1872, 1885
AcoI YGGCCR 3 cut(s) 353, 443, 2010
AcsI RAATTY 6 cut(s) 738, 1326, 1412, 1477, 1853, 2027
AcvI CACGTG 1 cut(s) 497
AcyI GRCGYC 1 cut(s) 690
AfiI CCNNNNNNNGG 4 cut(s) 257, 557, 1544, 2203
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 5 cut(s) 737, 1050, 1252, 1325, 1507
AhlI ACTAGT 1 cut(s) 709
AjnI CCWGG 5 cut(s) 381, 466, 556, 1149, 1383
AleI CACNNNNGTG 2 cut(s) 303, 1189
Alw21I GWGCWC 3 cut(s) 724, 855, 2107
Alw26I GTCTC 6 cut(s) 89, 377, 530, 681, 1246, 1459
AlwI GGATC 5 cut(s) 57, 1449, 1529, 1872, 1885
Aor13HI TCCGGA 2 cut(s) 63, 1606
AoxI GGCC 5 cut(s) 353, 443, 1387, 1420, 2010
ApeKI GCWGC 6 cut(s) 191, 222, 392, 605, 2017, 2087
ApoI RAATTY 6 cut(s) 738, 1326, 1412, 1477, 1853, 2027
AseI ATTAAT 2 cut(s) 212, 2051
AspLEI GCGC 2 cut(s) 786, 2119
AspS9I GGNCC 5 cut(s) 308, 560, 1203, 1387, 2209
AsuHPI GGTGA 2 cut(s) 1733, 1742
AsuII TTCGAA 3 cut(s) 656, 1222, 2031
AsuNHI GCTAGC 1 cut(s) 1624
AvaII GGWCC 4 cut(s) 308, 560, 1203, 2209
BaeGI GKGCMC 1 cut(s) 159
BaeI ACNNNNGTAYC 4 cut(s) 1289, 1322, 1932, 1965
BalI TGGCCA 3 cut(s) 355, 445, 2012
BamHI GGATCC 1 cut(s) 1877
BanI GGYRCC 1 cut(s) 1031
BanII GRGCYC 2 cut(s) 283, 855
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 3 cut(s) 345, 397, 2040
Bbv12I GWGCWC 3 cut(s) 724, 855, 2107
BbvCI CCTCAGC 1 cut(s) 10
BbvI GCAGC 6 cut(s) 178, 234, 379, 592, 2029, 2074
BccI CCATC 4 cut(s) 167, 618, 1221, 2161
BceAI ACGGC 4 cut(s) 499, 780, 926, 1019
BciT130I CCWGG 5 cut(s) 383, 468, 558, 1151, 1385
BciVI GTATCC 1 cut(s) 905
BclI TGATCA 2 cut(s) 238, 2229
BcoDI GTCTC 6 cut(s) 89, 377, 530, 681, 1246, 1459
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 4 cut(s) 710, 1625, 1892, 1907
BfmI CTRYAG 2 cut(s) 967, 1800
BfuAI ACCTGC 1 cut(s) 1792
BfuI GTATCC 1 cut(s) 905
BglII AGATCT 2 cut(s) 42, 760
BisI GCNGC 7 cut(s) 15, 192, 223, 393, 606, 2018, 2088
BlsI GCNGC 7 cut(s) 16, 193, 224, 394, 607, 2019, 2089
BmcAI AGTACT 3 cut(s) 590, 1106, 2202
Bme1390I CCNGG 5 cut(s) 383, 468, 558, 1151, 1385
Bme18I GGWCC 4 cut(s) 308, 560, 1203, 2209
BmgT120I GGNCC 5 cut(s) 308, 560, 1203, 1387, 2209
BmiI GGNNCC 5 cut(s) 18, 282, 555, 1033, 1879
BmrFI CCNGG 5 cut(s) 383, 468, 558, 1151, 1385
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 4 cut(s) 817, 1053, 1612, 1987
BmtI GCTAGC 1 cut(s) 1628
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 3 cut(s) 345, 397, 2040
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 3 cut(s) 1172, 1217, 2225
Bpu10I CCTNAGC 1 cut(s) 10
Bpu14I TTCGAA 3 cut(s) 656, 1222, 2031
BpuEI CTTGAG 5 cut(s) 20, 142, 512, 580, 1409
BsaAI YACGTR 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 690
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 5 cut(s) 301, 356, 744, 1384, 1567
BsaWI WCCGGW 5 cut(s) 63, 550, 562, 1244, 1606
BsaXI ACNNNNNCTCC 2 cut(s) 2130, 2160
Bsc4I CCNNNNNNNGG 4 cut(s) 257, 557, 1544, 2203
Bse1I ACTGG 4 cut(s) 191, 1473, 2091, 2208
Bse3DI GCAATG 4 cut(s) 89, 402, 1720, 2095
BseAI TCCGGA 2 cut(s) 63, 1606
BseBI CCWGG 5 cut(s) 383, 468, 558, 1151, 1385
BseDI CCNNGG 5 cut(s) 301, 356, 744, 1384, 1567
BseGI GGATG 5 cut(s) 589, 929, 1477, 1603, 2193
BseLI CCNNNNNNNGG 4 cut(s) 257, 557, 1544, 2203
BseMI GCAATG 4 cut(s) 89, 402, 1720, 2095
BseMII CTCAG 5 cut(s) 24, 663, 840, 1533, 1674
BseNI ACTGG 4 cut(s) 191, 1473, 2091, 2208
BseRI GAGGAG 2 cut(s) 583, 2201
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 6 cut(s) 178, 234, 379, 592, 2029, 2074
BseYI CCCAGC 1 cut(s) 676
Bsh1236I CGCG 1 cut(s) 2117
Bsh1285I CGRYCG 1 cut(s) 1089
BshFI GGCC 5 cut(s) 355, 445, 1389, 1422, 2012
BshNI GGYRCC 1 cut(s) 1031
BsiEI CGRYCG 1 cut(s) 1089
BsiHKAI GWGCWC 3 cut(s) 724, 855, 2107
BsiSI CCGG 5 cut(s) 64, 551, 563, 1245, 1607
BslFI GGGAC 1 cut(s) 1549
BslI CCNNNNNNNGG 4 cut(s) 257, 557, 1544, 2203
BsmAI GTCTC 6 cut(s) 89, 377, 530, 681, 1246, 1459
BsmBI CGTCTC 1 cut(s) 681
BsmFI GGGAC 1 cut(s) 1549
BsmI GAATGC 2 cut(s) 272, 1659
BsnI GGCC 5 cut(s) 355, 445, 1389, 1422, 2012
Bso31I GGTCTC 1 cut(s) 377
Bsp119I TTCGAA 3 cut(s) 656, 1222, 2031
Bsp1286I GDGCHC 5 cut(s) 159, 283, 724, 855, 2107
Bsp13I TCCGGA 2 cut(s) 63, 1606
Bsp1407I TGTACA 1 cut(s) 876
Bsp19I CCATGG 1 cut(s) 744
BspACI CCGC 3 cut(s) 14, 1201, 1805
BspANI GGCC 5 cut(s) 355, 445, 1389, 1422, 2012
BspCNI CTCAG 5 cut(s) 23, 662, 841, 1532, 1675
BspEI TCCGGA 2 cut(s) 63, 1606
BspFNI CGCG 1 cut(s) 2117
BspHI TCATGA 1 cut(s) 1137
BspLI GGNNCC 5 cut(s) 18, 282, 555, 1033, 1879
BspMI ACCTGC 1 cut(s) 1792
BspOI GCTAGC 1 cut(s) 1628
BspPI GGATC 5 cut(s) 57, 1449, 1529, 1872, 1885
BspT104I TTCGAA 3 cut(s) 656, 1222, 2031
BspT107I GGYRCC 1 cut(s) 1031
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 1201
BsrDI GCAATG 4 cut(s) 89, 402, 1720, 2095
BsrGI TGTACA 1 cut(s) 876
BsrI ACTGG 4 cut(s) 191, 1473, 2091, 2208
BssECI CCNNGG 5 cut(s) 301, 356, 744, 1384, 1567
BssNAI GTATAC 1 cut(s) 1099
BssNI GRCGYC 1 cut(s) 690
BssT1I CCWWGG 4 cut(s) 301, 356, 744, 1567
Bst1107I GTATAC 1 cut(s) 1099
Bst2UI CCWGG 5 cut(s) 383, 468, 558, 1151, 1385
Bst4CI ACNGT 6 cut(s) 441, 1109, 1310, 1334, 1339, 1837
Bst6I CTCTTC 2 cut(s) 116, 458
BstACI GRCGYC 1 cut(s) 690
BstAUI TGTACA 1 cut(s) 876
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 3 cut(s) 656, 1222, 2031
BstC8I GCNNGC 8 cut(s) 180, 220, 248, 488, 514, 1626, 1630, 2173
BstDEI CTNAG 6 cut(s) 10, 649, 849, 1400, 1519, 1683
BstDSI CCRYGG 1 cut(s) 744
BstF5I GGATG 5 cut(s) 589, 929, 1477, 1603, 2193
BstFNI CGCG 1 cut(s) 2117
BstHHI GCGC 2 cut(s) 786, 2119
BstMAI GTCTC 6 cut(s) 89, 377, 530, 681, 1246, 1459
BstMCI CGRYCG 1 cut(s) 1089
BstMWI GCNNNNNNNGC 6 cut(s) 11, 188, 200, 278, 792, 993
BstNI CCWGG 5 cut(s) 383, 468, 558, 1151, 1385
BstNSI RCATGY 2 cut(s) 1632, 2004
BstSCI CCNGG 5 cut(s) 381, 466, 556, 1149, 1383
BstSFI CTRYAG 2 cut(s) 967, 1800
BstSLI GKGCMC 1 cut(s) 159
BstUI CGCG 1 cut(s) 2117
BstV1I GCAGC 6 cut(s) 178, 234, 379, 592, 2029, 2074
BstV2I GAAGAC 3 cut(s) 345, 397, 2040
BstX2I RGATCY 4 cut(s) 42, 760, 1454, 1877
BstXI CCANNNNNNTGG 1 cut(s) 166
BstYI RGATCY 4 cut(s) 42, 760, 1454, 1877
BstZ17I GTATAC 1 cut(s) 1099
BsuI GTATCC 1 cut(s) 905
BsuRI GGCC 5 cut(s) 355, 445, 1389, 1422, 2012
BtgI CCRYGG 1 cut(s) 744
BtgZI GCGATG 1 cut(s) 922
BtsCI GGATG 5 cut(s) 589, 929, 1477, 1603, 2193
BtsIMutI CAGTG 3 cut(s) 59, 234, 1128
BveI ACCTGC 1 cut(s) 1792
Cac8I GCNNGC 8 cut(s) 180, 220, 248, 488, 514, 1626, 1630, 2173
CciI TCATGA 1 cut(s) 1137
CfoI GCGC 2 cut(s) 786, 2119
Cfr13I GGNCC 5 cut(s) 308, 560, 1203, 1387, 2209
CseI GACGC 1 cut(s) 1665
DdeI CTNAG 6 cut(s) 10, 649, 849, 1400, 1519, 1683
DraI TTTAAA 1 cut(s) 1861
EaeI YGGCCR 3 cut(s) 353, 443, 2010
Eam1104I CTCTTC 2 cut(s) 116, 458
EarI CTCTTC 2 cut(s) 116, 458
Ecl136II GAGCTC 1 cut(s) 853
Eco130I CCWWGG 4 cut(s) 301, 356, 744, 1567
Eco24I GRGCYC 2 cut(s) 283, 855
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 4 cut(s) 308, 560, 1203, 2209
Eco53kI GAGCTC 1 cut(s) 853
Eco72I CACGTG 1 cut(s) 497
EcoICRI GAGCTC 1 cut(s) 853
EcoRII CCWGG 5 cut(s) 381, 466, 556, 1149, 1383
EcoT14I CCWWGG 4 cut(s) 301, 356, 744, 1567
EcoT22I ATGCAT 1 cut(s) 2002
EcoT38I GRGCYC 2 cut(s) 283, 855
ErhI CCWWGG 4 cut(s) 301, 356, 744, 1567
Esp3I CGTCTC 1 cut(s) 681
FaqI GGGAC 1 cut(s) 1549
FauI CCCGC 1 cut(s) 1798
FauNDI CATATG 1 cut(s) 2083
FbaI TGATCA 2 cut(s) 238, 2229
FblI GTMKAC 1 cut(s) 1098
Fnu4HI GCNGC 7 cut(s) 15, 192, 223, 393, 606, 2018, 2088
FokI GGATG 5 cut(s) 596, 916, 1484, 1590, 2180
FriOI GRGCYC 2 cut(s) 283, 855
Fsp4HI GCNGC 7 cut(s) 15, 192, 223, 393, 606, 2018, 2088
FspBI CTAG 4 cut(s) 710, 1625, 1892, 1907
GlaI GCGC 2 cut(s) 785, 2118
GluI GCNGC 7 cut(s) 15, 192, 223, 393, 606, 2018, 2088
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 3 cut(s) 1172, 1217, 2225
HaeIII GGCC 5 cut(s) 355, 445, 1389, 1422, 2012
HapII CCGG 5 cut(s) 64, 551, 563, 1245, 1607
HgaI GACGC 1 cut(s) 1665
HhaI GCGC 2 cut(s) 786, 2119
Hin1I GRCGYC 1 cut(s) 690
Hin6I GCGC 2 cut(s) 784, 2117
HinP1I GCGC 2 cut(s) 784, 2117
HincII GTYRAC 1 cut(s) 1705
HindII GTYRAC 1 cut(s) 1705
HpaII CCGG 5 cut(s) 64, 551, 563, 1245, 1607
HphI GGTGA 2 cut(s) 1733, 1742
Hpy166II GTNNAC 4 cut(s) 879, 1099, 1306, 1705
Hpy8I GTNNAC 4 cut(s) 879, 1099, 1306, 1705
Hpy99I CGWCG 2 cut(s) 909, 1659
HpyAV CCTTC 4 cut(s) 109, 632, 677, 943
HpyCH4III ACNGT 6 cut(s) 441, 1109, 1310, 1334, 1339, 1837
HpyCH4IV ACGT 5 cut(s) 402, 496, 668, 690, 1745
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 188, 200, 278, 792, 993
HpyF3I CTNAG 6 cut(s) 10, 649, 849, 1400, 1519, 1683
HpySE526I ACGT 5 cut(s) 402, 496, 668, 690, 1745
Hsp92I GRCGYC 1 cut(s) 690
HspAI GCGC 2 cut(s) 784, 2117
Kpn2I TCCGGA 2 cut(s) 63, 1606
Ksp22I TGATCA 2 cut(s) 238, 2229
LmnI GCTCC 4 cut(s) 22, 66, 286, 1198
Lsp1109I GCAGC 6 cut(s) 178, 234, 379, 592, 2029, 2074
LweI GCATC 4 cut(s) 817, 1053, 1612, 1987
MaeI CTAG 4 cut(s) 710, 1625, 1892, 1907
MaeII ACGT 5 cut(s) 402, 496, 668, 690, 1745
MaeIII GTNAC 6 cut(s) 664, 1126, 1642, 1919, 2146, 2160
MbiI CCGCTC 1 cut(s) 1201
MfeI CAATTG 3 cut(s) 327, 840, 2120
MflI RGATCY 4 cut(s) 42, 760, 1454, 1877
MhlI GDGCHC 5 cut(s) 159, 283, 724, 855, 2107
MlsI TGGCCA 3 cut(s) 355, 445, 2012
MluNI TGGCCA 3 cut(s) 355, 445, 2012
MlyI GAGTC 2 cut(s) 465, 2030
MmeI TCCRAC 2 cut(s) 985, 1321
Mox20I TGGCCA 3 cut(s) 355, 445, 2012
Mph1103I ATGCAT 1 cut(s) 2002
MroI TCCGGA 2 cut(s) 63, 1606
MscI TGGCCA 3 cut(s) 355, 445, 2012
MslI CAYNNNNRTG 3 cut(s) 303, 1189, 1664
Msp20I TGGCCA 3 cut(s) 355, 445, 2012
MspA1I CMGCKG 2 cut(s) 14, 2020
MspI CCGG 5 cut(s) 64, 551, 563, 1245, 1607
MspR9I CCNGG 5 cut(s) 383, 468, 558, 1151, 1385
MunI CAATTG 3 cut(s) 327, 840, 2120
Mva1269I GAATGC 2 cut(s) 272, 1659
MvaI CCWGG 5 cut(s) 383, 468, 558, 1151, 1385
MvnI CGCG 1 cut(s) 2117
MwoI GCNNNNNNNGC 6 cut(s) 11, 188, 200, 278, 792, 993
NcoI CCATGG 1 cut(s) 744
NdeI CATATG 1 cut(s) 2083
NheI GCTAGC 1 cut(s) 1624
NlaIV GGNNCC 5 cut(s) 18, 282, 555, 1033, 1879
NmuCI GTSAC 3 cut(s) 664, 1126, 2146
NsiI ATGCAT 1 cut(s) 2002
NspI RCATGY 2 cut(s) 1632, 2004
NspV TTCGAA 3 cut(s) 656, 1222, 2031
OliI CACNNNNGTG 2 cut(s) 303, 1189
PaeI GCATGC 1 cut(s) 1632
PagI TCATGA 1 cut(s) 1137
PctI GAATGC 2 cut(s) 272, 1659
PfeI GAWTC 8 cut(s) 58, 653, 818, 1046, 1235, 1371, 1404, 1888
PflFI GACNNNGTC 1 cut(s) 1747
PflMI CCANNNNNTGG 2 cut(s) 257, 2203
PkrI GCNGC 7 cut(s) 16, 193, 224, 394, 607, 2019, 2089
Ple19I CGATCG 1 cut(s) 1089
PleI GAGTC 2 cut(s) 465, 2030
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 2 cut(s) 465, 2030
Ppu21I YACGTR 1 cut(s) 497
PshBI ATTAAT 2 cut(s) 212, 2051
Psp124BI GAGCTC 1 cut(s) 855
Psp6I CCWGG 5 cut(s) 381, 466, 556, 1149, 1383
PspCI CACGTG 1 cut(s) 497
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 5 cut(s) 381, 466, 556, 1149, 1383
PspN4I GGNNCC 5 cut(s) 18, 282, 555, 1033, 1879
PspPI GGNCC 5 cut(s) 308, 560, 1203, 1387, 2209
PsuI RGATCY 4 cut(s) 42, 760, 1454, 1877
PsyI GACNNNGTC 1 cut(s) 1747
PvuI CGATCG 1 cut(s) 1089
PvuII CAGCTG 1 cut(s) 2020
RseI CAYNNNNRTG 3 cut(s) 303, 1189, 1664
SacI GAGCTC 1 cut(s) 855
SatI GCNGC 7 cut(s) 15, 192, 223, 393, 606, 2018, 2088
Sau96I GGNCC 5 cut(s) 308, 560, 1203, 1387, 2209
ScaI AGTACT 3 cut(s) 590, 1106, 2202
SchI GAGTC 2 cut(s) 465, 2030
ScrFI CCNGG 5 cut(s) 383, 468, 558, 1151, 1385
SduI GDGCHC 5 cut(s) 159, 283, 724, 855, 2107
SfaNI GCATC 4 cut(s) 817, 1053, 1612, 1987
SfcI CTRYAG 2 cut(s) 967, 1800
SfuI TTCGAA 3 cut(s) 656, 1222, 2031
SinI GGWCC 4 cut(s) 308, 560, 1203, 2209
SmiMI CAYNNNNRTG 3 cut(s) 303, 1189, 1664
SmlI CTYRAG 5 cut(s) 35, 121, 527, 595, 1424
SmoI CTYRAG 5 cut(s) 35, 121, 527, 595, 1424
SpeI ACTAGT 1 cut(s) 709
SphI GCATGC 1 cut(s) 1632
SsiI CCGC 3 cut(s) 14, 1201, 1805
SspI AATATT 1 cut(s) 703
SspMI CTAG 4 cut(s) 710, 1625, 1892, 1907
SstI GAGCTC 1 cut(s) 855
StyD4I CCNGG 5 cut(s) 381, 466, 556, 1149, 1383
StyI CCWWGG 4 cut(s) 301, 356, 744, 1567
TaaI ACNGT 6 cut(s) 441, 1109, 1310, 1334, 1339, 1837
TaiI ACGT 5 cut(s) 405, 499, 671, 693, 1748
TaqI TCGA 9 cut(s) 137, 656, 771, 904, 1061, 1222, 1435, 2031, 2039
TaqII GACCGA 1 cut(s) 1668
TatI WGTACW 6 cut(s) 576, 588, 876, 1104, 1611, 2200
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 8 cut(s) 58, 653, 818, 1046, 1235, 1371, 1404, 1888
TscAI CASTG 3 cut(s) 59, 241, 1135
TseFI GTSAC 3 cut(s) 664, 1126, 2146
TseI GCWGC 6 cut(s) 191, 222, 392, 605, 2017, 2087
Tsp45I GTSAC 3 cut(s) 664, 1126, 2146
TspDTI ATGAA 9 cut(s) 61, 796, 882, 925, 1157, 1246, 1460, 1480, 2039
TspRI CASTG 3 cut(s) 59, 241, 1135
Tth111I GACNNNGTC 1 cut(s) 1747
Van91I CCANNNNNTGG 2 cut(s) 257, 2203
VpaK11BI GGWCC 4 cut(s) 308, 560, 1203, 2209
VspI ATTAAT 2 cut(s) 212, 2051
XapI RAATTY 6 cut(s) 738, 1326, 1412, 1477, 1853, 2027
XceI RCATGY 2 cut(s) 1632, 2004
XcmI CCANNNNNNNNNTGG 2 cut(s) 889, 1465
XmiI GTMKAC 1 cut(s) 1098
XspI CTAG 4 cut(s) 710, 1625, 1892, 1907
ZraI GACGTC 1 cut(s) 691
ZrmI AGTACT 3 cut(s) 590, 1106, 2202
Zsp2I ATGCAT 1 cut(s) 2002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.