Rmu_sc0004286.1_g000001

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004286.1
OG0000662
Physical Location & Seq
Forward (+)
28 .. 1449
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004286.1_g000001.1.cds

Sequence Viewer

Length: 1422 bp
atgtatgaagctctgtacaccaaagtttatgtggtacattctcgacggggatacttgttgagagccgcatccttcatcgccgtttgcatagcctttgttttcttctatagattagagaaacatggctttcacagctttgatgttggaattacttattccttgctttttggtgccatagctcttgattcagtagctctttcgatgctgatattatctgattggacgatcgctgttgtatacaagtactgtaaaaagtgtcgtgtagtcactgatctcatgatgaagtacctggagtttaagagatcatggcgaccaactactccaccaaagtgtctggagcggaccagaaagattttatttcggagatggtgtgaatctgtctccggtttcagcttcatagattattctctccgagaatgtggaagatcagtagtccgacctgcaattttggaccaaattgtcatttaccaaaggatcatcagatttttgggctacctttaccatagaatcatcatattcctaggcaacctttacctcagaatcattaaattctttggcctgaagtatcttcgagatgagatgaagtatagatccagcaagagactggaagagaatttgtggaagttcatttttgatgagttaaaaagtaaatcctcagaagtagttgatgatcccaaaattggtagagaaatatgttcgtcccaaggggactgggttctcctaaaaaagtatgagaagcatccggaatacaagagctttctatcttatgaggaattacaatcgtgctttgttgatgttacttatgaccgacgcgttctgctgtggcatattgctactgagatgtgctattacagagttgatacaggcaatggaggtgataatcgtaaatacagtaagacgttgtctgattatatgctatatctcttaataatgcagcctgctttgatgtcctctgtagcgggaatcggacaaaagagatttagagatacggttgcagaagccaataaattctttaaaaaaaggggattgggttccgatgtgaaactaagcgaggggaaactacgcgaggcttgttgcaaactccttgatgtagatacaggagttaaaccagttgatgtcaaagggaatagaagtaaatctgttttgtttgatgcatgtattttggccaggcagctgatgaaattccaagacaagaaatgggacttaataagctcggtgtgggtggaattgctatcatatgctgcaagtcattgcagagcacatagccacgcgcaattgctaactaaaggtggagagcttgtcacttttgtttggttactgatggctcgctttggcattgaggaggacatccaagtactggaggtccaagcaagagtaaaactgatcatggaaaagcaacctgatgtattccagagtaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

473

Amino Acids

55.3

Weight (kDa)

9.35

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 448
AccB1I GGYRCC 1 cut(s) 170
AccB7I CCANNNNNTGG 1 cut(s) 1357
AccBSI CCGCTC 1 cut(s) 340
AccI GTMKAC 1 cut(s) 237
AccII CGCG 3 cut(s) 813, 1065, 1271
AccIII TCCGGA 1 cut(s) 742
AciI CCGC 3 cut(s) 66, 340, 959
AclWI GGATC 3 cut(s) 482, 585, 665
AcoI YGGCCR 1 cut(s) 1164
AcsI RAATTY 4 cut(s) 548, 613, 1007, 1181
AcuI CTGAAG 1 cut(s) 581
AfaI GTAC 5 cut(s) 17, 36, 245, 287, 1356
AfiI CCNNNNNNNGG 2 cut(s) 680, 1357
AflIII ACRYGT 1 cut(s) 811
AjnI CCWGG 2 cut(s) 288, 1166
AleI CACNNNNGTG 1 cut(s) 328
AluBI AGCT 9 cut(s) 11, 135, 179, 194, 393, 756, 1174, 1212, 1297
AluI AGCT 9 cut(s) 11, 135, 179, 194, 393, 756, 1174, 1212, 1297
Alw21I GWGCWC 1 cut(s) 1261
Alw26I GTCTC 2 cut(s) 385, 595
AlwI GGATC 3 cut(s) 482, 585, 665
Aor13HI TCCGGA 1 cut(s) 742
AoxI GGCC 2 cut(s) 556, 1164
ApeKI GCWGC 3 cut(s) 934, 1171, 1241
ApoI RAATTY 4 cut(s) 548, 613, 1007, 1181
AspA2I CCTAGG 1 cut(s) 520
AspLEI GCGC 1 cut(s) 1273
AspS9I GGNCC 3 cut(s) 342, 451, 1363
AsuHPI GGTGA 1 cut(s) 887
AvaII GGWCC 3 cut(s) 342, 451, 1363
AvrII CCTAGG 1 cut(s) 520
BaeI ACNNNNGTAYC 2 cut(s) 1086, 1119
BalI TGGCCA 1 cut(s) 1166
BanI GGYRCC 1 cut(s) 170
Bbv12I GWGCWC 1 cut(s) 1261
BbvI GCAGC 3 cut(s) 946, 1183, 1228
BccI CCATC 2 cut(s) 360, 1315
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 2 cut(s) 290, 1168
BciVI GTATCC 1 cut(s) 44
BclI TGATCA 1 cut(s) 1383
BcoDI GTCTC 2 cut(s) 385, 595
BfaI CTAG 1 cut(s) 521
BfmI CTRYAG 2 cut(s) 106, 954
BfuAI ACCTGC 1 cut(s) 448
BfuI GTATCC 1 cut(s) 44
BisI GCNGC 4 cut(s) 66, 935, 1172, 1242
BlnI CCTAGG 1 cut(s) 520
BlsI GCNGC 4 cut(s) 67, 936, 1173, 1243
BmcAI AGTACT 2 cut(s) 245, 1356
Bme1390I CCNGG 2 cut(s) 290, 1168
Bme18I GGWCC 3 cut(s) 342, 451, 1363
BmgT120I GGNCC 3 cut(s) 342, 451, 1363
BmiI GGNNCC 2 cut(s) 172, 1033
BmrFI CCNGG 2 cut(s) 290, 1168
BmrI ACTGGG 1 cut(s) 721
BmsI GCATC 4 cut(s) 77, 192, 748, 1141
BmuI ACTGGG 1 cut(s) 721
BpmI CTGGAG 3 cut(s) 311, 356, 1379
BsaJI CCNNGG 2 cut(s) 520, 703
BsaWI WCCGGW 2 cut(s) 383, 742
BsaXI ACNNNNNCTCC 4 cut(s) 355, 385, 1284, 1314
Bsc4I CCNNNNNNNGG 2 cut(s) 680, 1357
Bse1I ACTGG 4 cut(s) 609, 716, 1109, 1362
Bse3DI GCAATG 2 cut(s) 874, 1249
BseAI TCCGGA 1 cut(s) 742
BseBI CCWGG 2 cut(s) 290, 1168
BseDI CCNNGG 2 cut(s) 520, 703
BseGI GGATG 3 cut(s) 68, 739, 1347
BseLI CCNNNNNNNGG 2 cut(s) 680, 1357
BseMI GCAATG 2 cut(s) 874, 1249
BseMII CTCAG 3 cut(s) 550, 669, 828
BseNI ACTGG 4 cut(s) 609, 716, 1109, 1362
BseRI GAGGAG 1 cut(s) 1355
BseXI GCAGC 3 cut(s) 946, 1183, 1228
Bsh1236I CGCG 3 cut(s) 813, 1065, 1271
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 2 cut(s) 558, 1166
BshNI GGYRCC 1 cut(s) 170
BsiEI CGRYCG 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 1261
BsiSI CCGG 2 cut(s) 384, 743
BslFI GGGAC 3 cut(s) 685, 722, 1214
BslI CCNNNNNNNGG 2 cut(s) 680, 1357
BsmAI GTCTC 2 cut(s) 385, 595
BsmFI GGGAC 3 cut(s) 685, 722, 1214
BsnI GGCC 2 cut(s) 558, 1166
Bsp1286I GDGCHC 1 cut(s) 1261
Bsp13I TCCGGA 1 cut(s) 742
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 8 cut(s) 225, 271, 302, 425, 474, 590, 670, 1383
BspACI CCGC 3 cut(s) 66, 340, 959
BspANI GGCC 2 cut(s) 558, 1166
BspCNI CTCAG 3 cut(s) 549, 668, 829
BspEI TCCGGA 1 cut(s) 742
BspFNI CGCG 3 cut(s) 813, 1065, 1271
BspHI TCATGA 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 172, 1033
BspMI ACCTGC 1 cut(s) 448
BspPI GGATC 3 cut(s) 482, 585, 665
BspT107I GGYRCC 1 cut(s) 170
BsrBI CCGCTC 1 cut(s) 340
BsrDI GCAATG 2 cut(s) 874, 1249
BsrGI TGTACA 1 cut(s) 15
BsrI ACTGG 4 cut(s) 609, 716, 1109, 1362
BssECI CCNNGG 2 cut(s) 520, 703
BssMI GATC 8 cut(s) 225, 271, 302, 425, 474, 590, 670, 1383
BssNAI GTATAC 1 cut(s) 238
BssT1I CCWWGG 2 cut(s) 520, 703
Bst1107I GTATAC 1 cut(s) 238
Bst2UI CCWGG 2 cut(s) 290, 1168
Bst4CI ACNGT 3 cut(s) 248, 893, 991
Bst6I CTCTTC 1 cut(s) 603
BstAUI TGTACA 1 cut(s) 15
BstC8I GCNNGC 2 cut(s) 939, 1327
BstDEI CTNAG 4 cut(s) 536, 655, 837, 1046
BstF5I GGATG 3 cut(s) 68, 739, 1347
BstFNI CGCG 3 cut(s) 813, 1065, 1271
BstHHI GCGC 1 cut(s) 1273
BstKTI GATC 8 cut(s) 228, 274, 305, 428, 477, 593, 673, 1386
BstMAI GTCTC 2 cut(s) 385, 595
BstMBI GATC 8 cut(s) 225, 271, 302, 425, 474, 590, 670, 1383
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 2 cut(s) 290, 1168
BstNSI RCATGY 1 cut(s) 1158
BstSCI CCNGG 2 cut(s) 288, 1166
BstSFI CTRYAG 2 cut(s) 106, 954
BstUI CGCG 3 cut(s) 813, 1065, 1271
BstV1I GCAGC 3 cut(s) 946, 1183, 1228
BstX2I RGATCY 1 cut(s) 590
BstYI RGATCY 1 cut(s) 590
BstZ17I GTATAC 1 cut(s) 238
BsuI GTATCC 1 cut(s) 44
BsuRI GGCC 2 cut(s) 558, 1166
BtgZI GCGATG 1 cut(s) 61
BtsCI GGATG 3 cut(s) 68, 739, 1347
BtsIMutI CAGTG 1 cut(s) 267
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 2 cut(s) 939, 1327
CciI TCATGA 1 cut(s) 276
CfoI GCGC 1 cut(s) 1273
Cfr13I GGNCC 3 cut(s) 342, 451, 1363
CseI GACGC 1 cut(s) 819
Csp6I GTAC 5 cut(s) 16, 35, 244, 286, 1355
CviAII CATG 5 cut(s) 122, 277, 306, 1155, 1387
CviQI GTAC 5 cut(s) 16, 35, 244, 286, 1355
DdeI CTNAG 4 cut(s) 536, 655, 837, 1046
DpnI GATC 8 cut(s) 227, 273, 304, 427, 476, 592, 672, 1385
DpnII GATC 8 cut(s) 225, 271, 302, 425, 474, 590, 670, 1383
DraI TTTAAA 1 cut(s) 1015
DrdI GACNNNNNNGTC 1 cut(s) 458
DseDI GACNNNNNNGTC 1 cut(s) 458
EaeI YGGCCR 1 cut(s) 1164
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Eco130I CCWWGG 2 cut(s) 520, 703
Eco47I GGWCC 3 cut(s) 342, 451, 1363
Eco57I CTGAAG 1 cut(s) 581
EcoRII CCWGG 2 cut(s) 288, 1166
EcoT14I CCWWGG 2 cut(s) 520, 703
EcoT22I ATGCAT 1 cut(s) 1156
ErhI CCWWGG 2 cut(s) 520, 703
FaeI CATG 5 cut(s) 125, 280, 309, 1158, 1390
FaqI GGGAC 3 cut(s) 685, 722, 1214
FatI CATG 5 cut(s) 121, 276, 305, 1154, 1386
FauI CCCGC 1 cut(s) 952
FauNDI CATATG 1 cut(s) 1237
FbaI TGATCA 1 cut(s) 1383
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 4 cut(s) 66, 935, 1172, 1242
FokI GGATG 3 cut(s) 55, 726, 1334
Fsp4HI GCNGC 4 cut(s) 66, 935, 1172, 1242
FspBI CTAG 1 cut(s) 521
GlaI GCGC 1 cut(s) 1272
GluI GCNGC 4 cut(s) 66, 935, 1172, 1242
GsuI CTGGAG 3 cut(s) 311, 356, 1379
HaeIII GGCC 2 cut(s) 558, 1166
HapII CCGG 2 cut(s) 384, 743
HgaI GACGC 1 cut(s) 819
HhaI GCGC 1 cut(s) 1273
Hin1II CATG 5 cut(s) 125, 280, 309, 1158, 1390
Hin6I GCGC 1 cut(s) 1271
HinP1I GCGC 1 cut(s) 1271
HinfI GANTC 5 cut(s) 185, 374, 507, 540, 963
HpaII CCGG 2 cut(s) 384, 743
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 2 cut(s) 18, 238
Hpy188III TCNNGA 7 cut(s) 42, 182, 277, 335, 572, 743, 1411
Hpy8I GTNNAC 2 cut(s) 18, 238
Hpy99I CGWCG 2 cut(s) 48, 813
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 3 cut(s) 248, 893, 991
HpyCH4IV ACGT 1 cut(s) 899
HpyCH4V TGCA 8 cut(s) 87, 443, 934, 995, 1077, 1154, 1244, 1254
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpyF3I CTNAG 4 cut(s) 536, 655, 837, 1046
HpySE526I ACGT 1 cut(s) 899
Hsp92II CATG 5 cut(s) 125, 280, 309, 1158, 1390
HspAI GCGC 1 cut(s) 1271
Kpn2I TCCGGA 1 cut(s) 742
Ksp22I TGATCA 1 cut(s) 1383
Kzo9I GATC 8 cut(s) 225, 271, 302, 425, 474, 590, 670, 1383
LmnI GCTCC 1 cut(s) 337
Lsp1109I GCAGC 3 cut(s) 946, 1183, 1228
LweI GCATC 4 cut(s) 77, 192, 748, 1141
MaeI CTAG 1 cut(s) 521
MaeII ACGT 1 cut(s) 899
MaeIII GTNAC 4 cut(s) 265, 796, 1300, 1314
MalI GATC 8 cut(s) 227, 273, 304, 427, 476, 592, 672, 1385
MbiI CCGCTC 1 cut(s) 340
MboI GATC 8 cut(s) 225, 271, 302, 425, 474, 590, 670, 1383
MboII GAAGA 4 cut(s) 94, 435, 560, 620
MfeI CAATTG 1 cut(s) 1274
MflI RGATCY 1 cut(s) 590
MhlI GDGCHC 1 cut(s) 1261
MlsI TGGCCA 1 cut(s) 1166
MluI ACGCGT 1 cut(s) 811
MluNI TGGCCA 1 cut(s) 1166
MmeI TCCRAC 2 cut(s) 124, 460
Mox20I TGGCCA 1 cut(s) 1166
Mph1103I ATGCAT 1 cut(s) 1156
MroI TCCGGA 1 cut(s) 742
MscI TGGCCA 1 cut(s) 1166
MseI TTAA 7 cut(s) 297, 546, 641, 926, 1014, 1104, 1205
MslI CAYNNNNRTG 1 cut(s) 328
Msp20I TGGCCA 1 cut(s) 1166
MspA1I CMGCKG 1 cut(s) 1174
MspI CCGG 2 cut(s) 384, 743
MspR9I CCNGG 2 cut(s) 290, 1168
MunI CAATTG 1 cut(s) 1274
MvaI CCWGG 2 cut(s) 290, 1168
MvnI CGCG 3 cut(s) 813, 1065, 1271
MwoI GCNNNNNNNGC 1 cut(s) 132
NdeI CATATG 1 cut(s) 1237
NdeII GATC 8 cut(s) 225, 271, 302, 425, 474, 590, 670, 1383
NlaIII CATG 5 cut(s) 125, 280, 309, 1158, 1390
NlaIV GGNNCC 2 cut(s) 172, 1033
NmuCI GTSAC 2 cut(s) 265, 1300
NsiI ATGCAT 1 cut(s) 1156
NspI RCATGY 1 cut(s) 1158
OliI CACNNNNGTG 1 cut(s) 328
PagI TCATGA 1 cut(s) 276
PfeI GAWTC 5 cut(s) 185, 374, 507, 540, 963
PflFI GACNNNGTC 1 cut(s) 901
PflMI CCANNNNNTGG 1 cut(s) 1357
PkrI GCNGC 4 cut(s) 67, 936, 1173, 1243
Ple19I CGATCG 1 cut(s) 228
Psp6I CCWGG 2 cut(s) 288, 1166
PspGI CCWGG 2 cut(s) 288, 1166
PspN4I GGNNCC 2 cut(s) 172, 1033
PspPI GGNCC 3 cut(s) 342, 451, 1363
PsuI RGATCY 1 cut(s) 590
PsyI GACNNNGTC 1 cut(s) 901
PvuI CGATCG 1 cut(s) 228
PvuII CAGCTG 1 cut(s) 1174
RsaI GTAC 5 cut(s) 17, 36, 245, 287, 1356
RsaNI GTAC 5 cut(s) 16, 35, 244, 286, 1355
RseI CAYNNNNRTG 1 cut(s) 328
SaqAI TTAA 7 cut(s) 297, 546, 641, 926, 1014, 1104, 1205
SatI GCNGC 4 cut(s) 66, 935, 1172, 1242
Sau3AI GATC 8 cut(s) 225, 271, 302, 425, 474, 590, 670, 1383
Sau96I GGNCC 3 cut(s) 342, 451, 1363
ScaI AGTACT 2 cut(s) 245, 1356
ScrFI CCNGG 2 cut(s) 290, 1168
SduI GDGCHC 1 cut(s) 1261
SfaNI GCATC 4 cut(s) 77, 192, 748, 1141
SfcI CTRYAG 2 cut(s) 106, 954
SinI GGWCC 3 cut(s) 342, 451, 1363
SmiMI CAYNNNNRTG 1 cut(s) 328
SsiI CCGC 3 cut(s) 66, 340, 959
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 2 cut(s) 288, 1166
StyI CCWWGG 2 cut(s) 520, 703
TaaI ACNGT 3 cut(s) 248, 893, 991
TaiI ACGT 1 cut(s) 902
TaqI TCGA 3 cut(s) 43, 200, 571
TaqII GACCGA 1 cut(s) 822
TatI WGTACW 3 cut(s) 15, 243, 1354
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 5 cut(s) 185, 374, 507, 540, 963
Tru1I TTAA 7 cut(s) 297, 546, 641, 926, 1014, 1104, 1205
Tru9I TTAA 7 cut(s) 297, 546, 641, 926, 1014, 1104, 1205
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 2 cut(s) 265, 1300
TseI GCWGC 3 cut(s) 934, 1171, 1241
Tsp45I GTSAC 2 cut(s) 265, 1300
TspDTI ATGAA 7 cut(s) 21, 64, 296, 385, 596, 616, 1193
TspRI CASTG 1 cut(s) 274
Tth111I GACNNNGTC 1 cut(s) 901
Van91I CCANNNNNTGG 1 cut(s) 1357
VpaK11BI GGWCC 3 cut(s) 342, 451, 1363
XapI RAATTY 4 cut(s) 548, 613, 1007, 1181
XceI RCATGY 1 cut(s) 1158
XcmI CCANNNNNNNNNTGG 2 cut(s) 28, 601
XmaJI CCTAGG 1 cut(s) 520
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 1 cut(s) 521
ZrmI AGTACT 2 cut(s) 245, 1356
Zsp2I ATGCAT 1 cut(s) 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.