Rh2BG251300

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000662
Physical Location & Seq
Reverse (-)
25863209 .. 25867074
3866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG251300.1

Sequence Viewer

Length: 2292 bp
ATGGGAGTTAAAGCAAGAGAGGATATGGCTTCATCAGCGGCTCCTATTTCTGACAGCCTCAAGAAGATCTGGGATAAGTGGAATCTCCGGAGCTTCATCATGTTAAGTCTCACATTGCAGACCTTCTTAATTCTCTTTGCACCCTTAAGAAAGCGATCATCGAGCAAATGGGTGATCGTGCCCACCTGGTTGGCTTACTTGCTCGCTGACTGGGCTGCTAACTTTGCTATCGGACTAATTGCAAGCAGCCAAACGAACACTGATCAAGGTTGGCCAGAGAATGGTAATCTATTGGCATTCTGGGCCCCCTTTCTTCTTTTGCACCTTGGTGGTCCAGACACCATAATTGCAATTGCTATGGAAGACAATGCGTTGTGGCCAAGGCACTTGCTTGGTCTCATATTCCAGGTTGTAGCTGCAATGTACGTCTTTGGTATGCAGTTCTCTAACCAAAACAAGCTACTGTGGCCAACACTCTTCCTGTTTGTACCAGGACTCGTCAAGTACGGCGAGCGAACACGTGCGTTATATCTTGCCAGCTTGGAAAGTCTCAAGGAAACCATACTTGCAGTACCGGAACCTGGACCGGATTATGCAAAGTACATGGATGAGTACTCCTCAAGAAAAGCTGCCCATCTTCCAATTAAAATAGAAATCAAAGAAGGTCGTAGCTCAGATTCGAAGAATGTGACGTATCGGCTGGGAGAAGGAGACGTCGTCATTGATAATATTACACTAGTGGAGGGTGCTCATCACTTGTTTCAAATTTCCATGGGTCTTATTATAGATCTTGTGTTCGACTATCATCAGCGCAAAGAAAGCCGTGCCTATTTCATCAAATTAGAATCAGGAGATGCTTTTACATCAATTGCTACTGAGCTCAATTTTATGTATGAAGCTCTGTACACCAAAGTTTATGTGGTACATTCTCGACGGGGATACTTGTTGAGAGCCGCATCCTTCATCGCCGTTTGCATAGCCTTTTTTTTCTTCTATAGATTAGAGAAACATGGCTTTCACAGCTTTGATGTTGGAATTACTTATTCCTTGCTTTTTGGTGCCATAGCTCTTGATTCAATAGCTCTTTTGATGCTGATATTATCTGATTGGACGATCGCTGTTGTATACAAGTACTGTAAAAAGTGTCGTGTAGTCACTGATCTCATGATGAAGTACCTGGAATTTAAGAGATCATGGCGACCAACTACTCCACCAAAGTGTCTGGAGCGGACCAGAAAGATTTTATTTCGGAGATGGTGTGAATCTGTCTCCGGTTTCAGCTTCATAGATTATTCTCTCCGAGAATGTGGAAGATCAGTAGTCCGACCTGCAATTTTGGACCAAATTGGCATTTACCGAAGGATCATCAGATTTTTGGGCTACCTTTACCATAGAATCATCATATTCCTAGGCCACCTTTACCTCAGAATCATTAAATTCTTTGGCCTCAAGTATCTTCGAGATGAGATGAAGTACAGATCCAGCAAGAGACTGGAAGAGAATTTGTGGAAATTTATTTTTGATGAGTTGAAAAGTAAATCCTCAGAAGTTGTTGATGATCCCAAAATTGGTAGAGAAATATGTTCATCCCAAGGGGATTGGGTTCTCCTAAAAAAGTACGAGAACCATCCGGAGTACAATAACTTGCTAGCATGCTTTGTTGATGTTACTTATGACCGACGCGTTCTGATGTGGCATATTGCTACTGAGATGTGCTATTACAGAGTTGACACAGGCAATGGCGGTGAGCATGGTGAATATAGTAAGACGTTGTCTGATTATTTGCTATATCTCTTAATTATGCAACCTGCTTTGATGTCCTCTGTAGCGGGAATTGGACAAAAGAGATTTAGAGATACGGTTGCAGAAGCCAAAAAATTCTTCAAAAAAGGGCGATTGGGATCCGATGTGAAACTAGGCGAGGGAAAACTAGGCGAGGCTTGCTACAAACTCCTTGATATAGATACAGGAGTTAAACCTGTCAATGTCAAAGGGAATAGAAGTAAATCTGTTTTGTTTGATGCATGTATTCTGGCCAAGCAGCTGATGAAATTCGAAGACTCGAAATGGGAATTAATAAGCTCAGTGTGGGTGGAATTGCTATCATATGCTGCAAGTCATTGCAGAGCACAAAGCCACGCACAATTGCTGACTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTACTGATGGCTCGCTTTGGCATTGAGGAGGACATCCAAGTACTGGAGGTCCAAGCAAGAGTAAAACTGATCATGGAAAAGCAACCTGATGTATTCAAGAGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

763

Amino Acids

87.77

Weight (kDa)

9.11

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 63 - 431 4e-90 Domain of unknown function (DUF4220)
DUF594 PF04578 686 - 736 7.7e-22 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 717
Acc36I ACCTGC 2 cut(s) 1336, 1816
AccB1I GGYRCC 1 cut(s) 1058
AccB7I CCANNNNNTGG 2 cut(s) 281, 2227
AccBSI CCGCTC 1 cut(s) 1228
AccI GTMKAC 1 cut(s) 1125
AccII CGCG 1 cut(s) 1683
AccIII TCCGGA 2 cut(s) 87, 1630
AciI CCGC 5 cut(s) 38, 954, 1228, 1743, 1829
AclWI GGATC 5 cut(s) 1370, 1473, 1553, 1896, 1909
AcoI YGGCCR 4 cut(s) 272, 377, 467, 2034
AcsI RAATTY 7 cut(s) 765, 1181, 1436, 1501, 1511, 1877, 2051
AcvI CACGTG 1 cut(s) 521
AcyI GRCGYC 1 cut(s) 714
AfiI CCNNNNNNNGG 4 cut(s) 281, 581, 1568, 2227
AflII CTTAAG 1 cut(s) 145
AflIII ACRYGT 2 cut(s) 518, 1681
AgsI TTSAA 5 cut(s) 764, 1077, 1531, 1885, 2281
AhlI ACTAGT 1 cut(s) 736
AjnI CCWGG 5 cut(s) 185, 405, 490, 580, 1176
AjuI GAANNNNNNNTTGG 2 cut(s) 1865, 1897
AleI CACNNNNGTG 2 cut(s) 327, 1216
Alw21I GWGCWC 3 cut(s) 751, 882, 2131
Alw26I GTCTC 6 cut(s) 113, 401, 554, 705, 1273, 1483
AlwI GGATC 5 cut(s) 1370, 1473, 1553, 1896, 1909
Aor13HI TCCGGA 2 cut(s) 87, 1630
AoxI GGCC 7 cut(s) 272, 303, 377, 467, 1411, 1444, 2034
ApaI GGGCCC 1 cut(s) 307
ApeKI GCWGC 6 cut(s) 215, 246, 416, 629, 2041, 2111
ApoI RAATTY 7 cut(s) 765, 1181, 1436, 1501, 1511, 1877, 2051
AseI ATTAAT 1 cut(s) 2075
AspA2I CCTAGG 1 cut(s) 1408
AspLEI GCGC 1 cut(s) 813
AspS9I GGNCC 7 cut(s) 303, 304, 332, 584, 1230, 1339, 2233
AsuHPI GGTGA 3 cut(s) 184, 1757, 1766
AsuII TTCGAA 2 cut(s) 680, 2055
AsuNHI GCTAGC 1 cut(s) 1648
AvaII GGWCC 5 cut(s) 332, 584, 1230, 1339, 2233
AvrII CCTAGG 1 cut(s) 1408
BaeGI GKGCMC 2 cut(s) 183, 307
BaeI ACNNNNGTAYC 2 cut(s) 1956, 1989
BalI TGGCCA 4 cut(s) 274, 379, 469, 2036
BamHI GGATCC 1 cut(s) 1901
BanI GGYRCC 1 cut(s) 1058
BanII GRGCYC 2 cut(s) 307, 882
BbrPI CACGTG 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 369, 2064
Bbv12I GWGCWC 3 cut(s) 751, 882, 2131
BbvI GCAGC 6 cut(s) 202, 258, 403, 616, 2053, 2098
BccI CCATC 4 cut(s) 642, 1248, 1635, 2185
BceAI ACGGC 3 cut(s) 523, 807, 953
BciT130I CCWGG 5 cut(s) 187, 407, 492, 582, 1178
BciVI GTATCC 1 cut(s) 932
BclI TGATCA 2 cut(s) 262, 2253
BcoDI GTCTC 6 cut(s) 113, 401, 554, 705, 1273, 1483
BcuI ACTAGT 1 cut(s) 736
BfaI CTAG 5 cut(s) 737, 1409, 1649, 1916, 1931
BfmI CTRYAG 2 cut(s) 994, 1824
BfrI CTTAAG 1 cut(s) 145
BfuAI ACCTGC 2 cut(s) 1336, 1816
BfuI GTATCC 1 cut(s) 932
BglII AGATCT 2 cut(s) 66, 787
BisI GCNGC 8 cut(s) 39, 216, 247, 417, 630, 954, 2042, 2112
BlnI CCTAGG 1 cut(s) 1408
BlsI GCNGC 8 cut(s) 40, 217, 248, 418, 631, 955, 2043, 2113
BmcAI AGTACT 3 cut(s) 614, 1133, 2226
Bme1390I CCNGG 5 cut(s) 187, 407, 492, 582, 1178
Bme18I GGWCC 5 cut(s) 332, 584, 1230, 1339, 2233
BmgT120I GGNCC 7 cut(s) 303, 304, 332, 584, 1230, 1339, 2233
BmiI GGNNCC 6 cut(s) 42, 305, 306, 579, 1060, 1903
BmrFI CCNGG 5 cut(s) 187, 407, 492, 582, 1178
BmrI ACTGGG 1 cut(s) 220
BmsI GCATC 4 cut(s) 844, 965, 1080, 2011
BmtI GCTAGC 1 cut(s) 1652
BmuI ACTGGG 1 cut(s) 220
BpiI GAAGAC 2 cut(s) 369, 2064
BplI GAGNNNNNCTC 2 cut(s) 602, 634
BpmI CTGGAG 2 cut(s) 1244, 2249
Bpu14I TTCGAA 2 cut(s) 680, 2055
BpuEI CTTGAG 4 cut(s) 44, 536, 604, 1433
BsaAI YACGTR 1 cut(s) 521
BsaBI GATNNNNATC 1 cut(s) 1900
BsaHI GRCGYC 1 cut(s) 714
BsaI GGTCTC 1 cut(s) 401
BsaJI CCNNGG 5 cut(s) 325, 380, 771, 1408, 1591
BsaWI WCCGGW 5 cut(s) 87, 574, 586, 1271, 1630
BsaXI ACNNNNNCTCC 6 cut(s) 702, 732, 1243, 1273, 2154, 2184
Bsc4I CCNNNNNNNGG 4 cut(s) 281, 581, 1568, 2227
Bse1I ACTGG 3 cut(s) 215, 1497, 2232
Bse3DI GCAATG 4 cut(s) 113, 426, 1744, 2119
Bse8I GATNNNNATC 1 cut(s) 1900
BseAI TCCGGA 2 cut(s) 87, 1630
BseBI CCWGG 5 cut(s) 187, 407, 492, 582, 1178
BseDI CCNNGG 5 cut(s) 325, 380, 771, 1408, 1591
BseGI GGATG 5 cut(s) 613, 956, 1586, 1627, 2217
BseJI GATNNNNATC 1 cut(s) 1900
BseLI CCNNNNNNNGG 4 cut(s) 281, 581, 1568, 2227
BseMI GCAATG 4 cut(s) 113, 426, 1744, 2119
BseMII CTCAG 6 cut(s) 687, 867, 1438, 1557, 1698, 2097
BseNI ACTGG 3 cut(s) 215, 1497, 2232
BseRI GAGGAG 2 cut(s) 607, 2225
BseSI GKGCMC 2 cut(s) 183, 307
BseXI GCAGC 6 cut(s) 202, 258, 403, 616, 2053, 2098
BseYI CCCAGC 1 cut(s) 700
Bsh1236I CGCG 1 cut(s) 1683
Bsh1285I CGRYCG 1 cut(s) 1116
BshFI GGCC 7 cut(s) 274, 305, 379, 469, 1413, 1446, 2036
BshNI GGYRCC 1 cut(s) 1058
BsiEI CGRYCG 1 cut(s) 1116
BsiHKAI GWGCWC 3 cut(s) 751, 882, 2131
BsiSI CCGG 5 cut(s) 88, 575, 587, 1272, 1631
BslI CCNNNNNNNGG 4 cut(s) 281, 581, 1568, 2227
BsmAI GTCTC 6 cut(s) 113, 401, 554, 705, 1273, 1483
BsmBI CGTCTC 1 cut(s) 705
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 7 cut(s) 274, 305, 379, 469, 1413, 1446, 2036
Bso31I GGTCTC 1 cut(s) 401
Bsp119I TTCGAA 2 cut(s) 680, 2055
Bsp120I GGGCCC 1 cut(s) 303
Bsp1286I GDGCHC 5 cut(s) 183, 307, 751, 882, 2131
Bsp13I TCCGGA 2 cut(s) 87, 1630
Bsp1407I TGTACA 1 cut(s) 903
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 5 cut(s) 38, 954, 1228, 1743, 1829
BspANI GGCC 7 cut(s) 274, 305, 379, 469, 1413, 1446, 2036
BspCNI CTCAG 6 cut(s) 686, 868, 1437, 1556, 1699, 2096
BspEI TCCGGA 2 cut(s) 87, 1630
BspFNI CGCG 1 cut(s) 1683
BspHI TCATGA 1 cut(s) 1164
BspLI GGNNCC 6 cut(s) 42, 305, 306, 579, 1060, 1903
BspMI ACCTGC 2 cut(s) 1336, 1816
BspOI GCTAGC 1 cut(s) 1652
BspPI GGATC 5 cut(s) 1370, 1473, 1553, 1896, 1909
BspT104I TTCGAA 2 cut(s) 680, 2055
BspT107I GGYRCC 1 cut(s) 1058
BspTI CTTAAG 1 cut(s) 145
BspTNI GGTCTC 1 cut(s) 401
BsrBI CCGCTC 1 cut(s) 1228
BsrDI GCAATG 4 cut(s) 113, 426, 1744, 2119
BsrGI TGTACA 1 cut(s) 903
BsrI ACTGG 3 cut(s) 215, 1497, 2232
BssECI CCNNGG 5 cut(s) 325, 380, 771, 1408, 1591
BssNAI GTATAC 1 cut(s) 1126
BssNI GRCGYC 1 cut(s) 714
BssT1I CCWWGG 5 cut(s) 325, 380, 771, 1408, 1591
Bst1107I GTATAC 1 cut(s) 1126
Bst2UI CCWGG 5 cut(s) 187, 407, 492, 582, 1178
Bst4CI ACNGT 3 cut(s) 465, 1136, 1861
Bst6I CTCTTC 2 cut(s) 482, 1491
BstACI GRCGYC 1 cut(s) 714
BstAFI CTTAAG 1 cut(s) 145
BstAUI TGTACA 1 cut(s) 903
BstBAI YACGTR 1 cut(s) 521
BstBI TTCGAA 2 cut(s) 680, 2055
BstC8I GCNNGC 8 cut(s) 204, 244, 512, 538, 1650, 1654, 1942, 2197
BstDEI CTNAG 6 cut(s) 673, 876, 1424, 1543, 1707, 2083
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 5 cut(s) 613, 956, 1586, 1627, 2217
BstFNI CGCG 1 cut(s) 1683
BstHHI GCGC 1 cut(s) 813
BstMAI GTCTC 6 cut(s) 113, 401, 554, 705, 1273, 1483
BstMCI CGRYCG 1 cut(s) 1116
BstMWI GCNNNNNNNGC 8 cut(s) 35, 212, 224, 302, 466, 819, 1020, 1941
BstNI CCWGG 5 cut(s) 187, 407, 492, 582, 1178
BstNSI RCATGY 2 cut(s) 1656, 2028
BstSCI CCNGG 5 cut(s) 185, 405, 490, 580, 1176
BstSFI CTRYAG 2 cut(s) 994, 1824
BstSLI GKGCMC 2 cut(s) 183, 307
BstUI CGCG 1 cut(s) 1683
BstV1I GCAGC 6 cut(s) 202, 258, 403, 616, 2053, 2098
BstV2I GAAGAC 2 cut(s) 369, 2064
BstX2I RGATCY 4 cut(s) 66, 787, 1478, 1901
BstXI CCANNNNNNTGG 1 cut(s) 190
BstYI RGATCY 4 cut(s) 66, 787, 1478, 1901
BstZ17I GTATAC 1 cut(s) 1126
BsuI GTATCC 1 cut(s) 932
BsuRI GGCC 7 cut(s) 274, 305, 379, 469, 1413, 1446, 2036
BtgI CCRYGG 1 cut(s) 771
BtgZI GCGATG 1 cut(s) 949
BtsCI GGATG 5 cut(s) 613, 956, 1586, 1627, 2217
BtsIMutI CAGTG 3 cut(s) 258, 1155, 2091
BveI ACCTGC 2 cut(s) 1336, 1816
Cac8I GCNNGC 8 cut(s) 204, 244, 512, 538, 1650, 1654, 1942, 2197
CciI TCATGA 1 cut(s) 1164
CfoI GCGC 1 cut(s) 813
Cfr13I GGNCC 7 cut(s) 303, 304, 332, 584, 1230, 1339, 2233
CseI GACGC 1 cut(s) 1689
CsiI ACCWGGT 1 cut(s) 185
DdeI CTNAG 6 cut(s) 673, 876, 1424, 1543, 1707, 2083
EaeI YGGCCR 4 cut(s) 272, 377, 467, 2034
Eam1104I CTCTTC 2 cut(s) 482, 1491
EarI CTCTTC 2 cut(s) 482, 1491
Ecl136II GAGCTC 1 cut(s) 880
Eco130I CCWWGG 5 cut(s) 325, 380, 771, 1408, 1591
Eco24I GRGCYC 2 cut(s) 307, 882
Eco31I GGTCTC 1 cut(s) 401
Eco47I GGWCC 5 cut(s) 332, 584, 1230, 1339, 2233
Eco53kI GAGCTC 1 cut(s) 880
Eco72I CACGTG 1 cut(s) 521
EcoICRI GAGCTC 1 cut(s) 880
EcoO109I RGGNCCY 1 cut(s) 304
EcoRII CCWGG 5 cut(s) 185, 405, 490, 580, 1176
EcoT14I CCWWGG 5 cut(s) 325, 380, 771, 1408, 1591
EcoT22I ATGCAT 1 cut(s) 2026
EcoT38I GRGCYC 2 cut(s) 307, 882
ErhI CCWWGG 5 cut(s) 325, 380, 771, 1408, 1591
Esp3I CGTCTC 1 cut(s) 705
FauI CCCGC 1 cut(s) 1822
FauNDI CATATG 1 cut(s) 2107
FbaI TGATCA 2 cut(s) 262, 2253
FblI GTMKAC 1 cut(s) 1125
Fnu4HI GCNGC 8 cut(s) 39, 216, 247, 417, 630, 954, 2042, 2112
FokI GGATG 5 cut(s) 620, 943, 1573, 1614, 2204
FriOI GRGCYC 2 cut(s) 307, 882
Fsp4HI GCNGC 8 cut(s) 39, 216, 247, 417, 630, 954, 2042, 2112
FspBI CTAG 5 cut(s) 737, 1409, 1649, 1916, 1931
GlaI GCGC 1 cut(s) 812
GluI GCNGC 8 cut(s) 39, 216, 247, 417, 630, 954, 2042, 2112
GsaI CCCAGC 1 cut(s) 704
GsuI CTGGAG 2 cut(s) 1244, 2249
HaeIII GGCC 7 cut(s) 274, 305, 379, 469, 1413, 1446, 2036
HapII CCGG 5 cut(s) 88, 575, 587, 1272, 1631
HgaI GACGC 1 cut(s) 1689
HhaI GCGC 1 cut(s) 813
Hin1I GRCGYC 1 cut(s) 714
Hin6I GCGC 1 cut(s) 811
HinP1I GCGC 1 cut(s) 811
HincII GTYRAC 1 cut(s) 1729
HindII GTYRAC 1 cut(s) 1729
HinfI GANTC 9 cut(s) 82, 495, 677, 845, 1073, 1262, 1395, 1428, 2060
HpaII CCGG 5 cut(s) 88, 575, 587, 1272, 1631
HphI GGTGA 3 cut(s) 184, 1757, 1766
Hpy166II GTNNAC 3 cut(s) 906, 1126, 1729
Hpy8I GTNNAC 3 cut(s) 906, 1126, 1729
Hpy99I CGWCG 3 cut(s) 719, 936, 1683
HpyAV CCTTC 5 cut(s) 133, 656, 701, 970, 1353
HpyCH4III ACNGT 3 cut(s) 465, 1136, 1861
HpyCH4IV ACGT 5 cut(s) 426, 520, 692, 714, 1769
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 212, 224, 302, 466, 819, 1020, 1941
HpyF3I CTNAG 6 cut(s) 673, 876, 1424, 1543, 1707, 2083
HpySE526I ACGT 5 cut(s) 426, 520, 692, 714, 1769
Hsp92I GRCGYC 1 cut(s) 714
HspAI GCGC 1 cut(s) 811
Kpn2I TCCGGA 2 cut(s) 87, 1630
Ksp22I TGATCA 2 cut(s) 262, 2253
LmnI GCTCC 3 cut(s) 46, 90, 1225
Lsp1109I GCAGC 6 cut(s) 202, 258, 403, 616, 2053, 2098
LweI GCATC 4 cut(s) 844, 965, 1080, 2011
MabI ACCWGGT 1 cut(s) 185
MaeI CTAG 5 cut(s) 737, 1409, 1649, 1916, 1931
MaeII ACGT 5 cut(s) 426, 520, 692, 714, 1769
MaeIII GTNAC 5 cut(s) 688, 1153, 1666, 2170, 2184
MbiI CCGCTC 1 cut(s) 1228
MfeI CAATTG 3 cut(s) 351, 867, 2144
MflI RGATCY 4 cut(s) 66, 787, 1478, 1901
MhlI GDGCHC 5 cut(s) 183, 307, 751, 882, 2131
MlsI TGGCCA 4 cut(s) 274, 379, 469, 2036
MluI ACGCGT 1 cut(s) 1681
MluNI TGGCCA 4 cut(s) 274, 379, 469, 2036
MlyI GAGTC 2 cut(s) 489, 2054
MmeI TCCRAC 2 cut(s) 1012, 1348
Mox20I TGGCCA 4 cut(s) 274, 379, 469, 2036
Mph1103I ATGCAT 1 cut(s) 2026
MroI TCCGGA 2 cut(s) 87, 1630
MscI TGGCCA 4 cut(s) 274, 379, 469, 2036
MslI CAYNNNNRTG 2 cut(s) 327, 1216
Msp20I TGGCCA 4 cut(s) 274, 379, 469, 2036
MspA1I CMGCKG 2 cut(s) 38, 2044
MspCI CTTAAG 1 cut(s) 145
MspI CCGG 5 cut(s) 88, 575, 587, 1272, 1631
MspR9I CCNGG 5 cut(s) 187, 407, 492, 582, 1178
MunI CAATTG 3 cut(s) 351, 867, 2144
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 5 cut(s) 187, 407, 492, 582, 1178
MvnI CGCG 1 cut(s) 1683
MwoI GCNNNNNNNGC 8 cut(s) 35, 212, 224, 302, 466, 819, 1020, 1941
NcoI CCATGG 1 cut(s) 771
NdeI CATATG 1 cut(s) 2107
NheI GCTAGC 1 cut(s) 1648
NlaIV GGNNCC 6 cut(s) 42, 305, 306, 579, 1060, 1903
NmuCI GTSAC 3 cut(s) 688, 1153, 2170
NsiI ATGCAT 1 cut(s) 2026
NspI RCATGY 2 cut(s) 1656, 2028
NspV TTCGAA 2 cut(s) 680, 2055
OliI CACNNNNGTG 2 cut(s) 327, 1216
PaeI GCATGC 1 cut(s) 1656
PagI TCATGA 1 cut(s) 1164
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 7 cut(s) 82, 677, 845, 1073, 1262, 1395, 1428
PflFI GACNNNGTC 2 cut(s) 716, 1771
PflMI CCANNNNNTGG 2 cut(s) 281, 2227
PkrI GCNGC 8 cut(s) 40, 217, 248, 418, 631, 955, 2043, 2113
Ple19I CGATCG 1 cut(s) 1116
PleI GAGTC 2 cut(s) 489, 2054
PmaCI CACGTG 1 cut(s) 521
PmlI CACGTG 1 cut(s) 521
PpsI GAGTC 2 cut(s) 489, 2054
Ppu21I YACGTR 1 cut(s) 521
PshBI ATTAAT 1 cut(s) 2075
Psp124BI GAGCTC 1 cut(s) 882
Psp6I CCWGG 5 cut(s) 185, 405, 490, 580, 1176
PspCI CACGTG 1 cut(s) 521
PspFI CCCAGC 1 cut(s) 700
PspGI CCWGG 5 cut(s) 185, 405, 490, 580, 1176
PspN4I GGNNCC 6 cut(s) 42, 305, 306, 579, 1060, 1903
PspOMI GGGCCC 1 cut(s) 303
PspPI GGNCC 7 cut(s) 303, 304, 332, 584, 1230, 1339, 2233
PsuI RGATCY 4 cut(s) 66, 787, 1478, 1901
PsyI GACNNNGTC 2 cut(s) 716, 1771
PvuI CGATCG 1 cut(s) 1116
PvuII CAGCTG 1 cut(s) 2044
RseI CAYNNNNRTG 2 cut(s) 327, 1216
SacI GAGCTC 1 cut(s) 882
SatI GCNGC 8 cut(s) 39, 216, 247, 417, 630, 954, 2042, 2112
Sau96I GGNCC 7 cut(s) 303, 304, 332, 584, 1230, 1339, 2233
ScaI AGTACT 3 cut(s) 614, 1133, 2226
SchI GAGTC 2 cut(s) 489, 2054
ScrFI CCNGG 5 cut(s) 187, 407, 492, 582, 1178
SduI GDGCHC 5 cut(s) 183, 307, 751, 882, 2131
SexAI ACCWGGT 1 cut(s) 185
SfaNI GCATC 4 cut(s) 844, 965, 1080, 2011
SfcI CTRYAG 2 cut(s) 994, 1824
SfuI TTCGAA 2 cut(s) 680, 2055
SinI GGWCC 5 cut(s) 332, 584, 1230, 1339, 2233
SmiMI CAYNNNNRTG 2 cut(s) 327, 1216
SmlI CTYRAG 5 cut(s) 59, 145, 551, 619, 1448
SmoI CTYRAG 5 cut(s) 59, 145, 551, 619, 1448
SpeI ACTAGT 1 cut(s) 736
SphI GCATGC 1 cut(s) 1656
SsiI CCGC 5 cut(s) 38, 954, 1228, 1743, 1829
SspI AATATT 1 cut(s) 730
SspMI CTAG 5 cut(s) 737, 1409, 1649, 1916, 1931
SstI GAGCTC 1 cut(s) 882
StyD4I CCNGG 5 cut(s) 185, 405, 490, 580, 1176
StyI CCWWGG 5 cut(s) 325, 380, 771, 1408, 1591
TaaI ACNGT 3 cut(s) 465, 1136, 1861
TaiI ACGT 5 cut(s) 429, 523, 695, 717, 1772
TaqI TCGA 7 cut(s) 161, 680, 798, 931, 1459, 2055, 2063
TaqII GACCGA 1 cut(s) 1692
TatI WGTACW 7 cut(s) 600, 612, 903, 1131, 1473, 1635, 2224
TauI GCSGC 2 cut(s) 41, 956
TfiI GAWTC 7 cut(s) 82, 677, 845, 1073, 1262, 1395, 1428
TscAI CASTG 3 cut(s) 265, 1162, 2091
TseFI GTSAC 3 cut(s) 688, 1153, 2170
TseI GCWGC 6 cut(s) 215, 246, 416, 629, 2041, 2111
Tsp45I GTSAC 3 cut(s) 688, 1153, 2170
TspRI CASTG 3 cut(s) 265, 1162, 2091
Tth111I GACNNNGTC 2 cut(s) 716, 1771
Van91I CCANNNNNTGG 2 cut(s) 281, 2227
Vha464I CTTAAG 1 cut(s) 145
VpaK11BI GGWCC 5 cut(s) 332, 584, 1230, 1339, 2233
VspI ATTAAT 1 cut(s) 2075
XapI RAATTY 7 cut(s) 765, 1181, 1436, 1501, 1511, 1877, 2051
XceI RCATGY 2 cut(s) 1656, 2028
XcmI CCANNNNNNNNNTGG 2 cut(s) 916, 1489
XmaJI CCTAGG 1 cut(s) 1408
XmiI GTMKAC 1 cut(s) 1125
XspI CTAG 5 cut(s) 737, 1409, 1649, 1916, 1931
ZraI GACGTC 1 cut(s) 715
ZrmI AGTACT 3 cut(s) 614, 1133, 2226
Zsp2I ATGCAT 1 cut(s) 2026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.