Rroxscaffold_2G00132290

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000662
Physical Location & Seq
Forward (+)
69318093 .. 69327381
9289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132290.1

Sequence Viewer

Length: 2151 bp
ATGCAGTCAACTCGAGAAAACAAGCTATGGTGGCCAACACTCTTGCTAATTTTCCCAGGACTTGTCAAGTATGGCAAGCGAACACGTGCTTTATATCTTGCCATCTTCCGCAGTCTCAAGGACTCCATACTTGCAGTACCGGTCATTGGACCGAATTATGCAAAGTACATGGATGAGTACTCCGCAAGAAAAGCTGCCAATCTTCCAGTTCATATACACATCGAATTCGGCGGTGTCCCAGAATCCAAGAGTGAGATTTATCCGGTGGAAGAATACGGCTACAACATTAATAATAATATTGATGTGGTGAAACGTGCTTATCAATTCTTTAAAATATCCATCGCTCTTATTATAGATCTTCTTTTTGACTTTCGTCAGCGGGACAAGAGCCGTGCCTATTTTTGTCAGAGTAACCCAGAACACGCTTATACATTAATCGCGACAGAGCTCAACTTTATGTATGAAGCTCTTTACACCAAAGCTTATCTGGTGCATTCTCGGCGGGGGTATGTTTTAAGGGCTATATCATTTGGAACGGTTTGTATAGCCCTTGGTTTCTTCTATAAGCTGGACAAACATAGCTTCCACAGGTTTGATGTTAGAGTTACCTATTCCTTGCTCTTGGGTGCCATAGCCTTGGATTCAACAGCTATTTCGATGCTTATCCTATCTGATTGGACTGTCATAAAGTGTGGCCATAAGTCTTGTTCTTTAGCCATTAACCTCATGCAAAAATACCTCAACTTGAAGAGATCTTGGTTGTCTACTACTGAAAAGACAATAAAGTGGAGGATCTTATTTGGAAGATGGTCTGAATCTGTGTCTACATTCAGCTTTATAGATTATTCTCTCAAAGAATGCGGAAGAGTGCCACCTACAGTTATGGACTATGTAGGCTTTTCGGGCACTAAAATAATCAGAATCATGGGGCTCAAGGATATTCGAGATGAGTTGAAATATAGATCCCGCAAAGAACTCTCTGAGGAACTGTGGAAGTTCATTTTTGAAGAGTTGAAAAGTAAATCGGAGGATGCAGTTGATCCTCAAAATGGCAGCAAAATATGTTCAGCAAGAGGGGAGTCGATTCTTAGGAAGTACTACCATGGGGATGGCGGGCCACTGTTTAACAATTTAAGATCGCGAGTTTATCTTGGCGATGATGTTACTTATGATCGAAGCATTCTGTTATGGCACATTGCAACCGAGTTGCGCTATCACAATGAGAAGCCTCAGAATAGCAATGCTGGTGATCATAAGAATAGCAATGCTGGTGATGATGATGATGGTGCTCATCGAGAGTTTAGTAAGACATTGTCTGATTATATGTTATATCTCTTAGTTTTGCAGCCTAATTTGATGTCTTCTGTAGCGGGAATTGGTCAGAAAAGATTTTCTGATACTTTTGCAGAAGCCAAACAATTCTTTGAAAAAAGGGCGCTGGGATCGGTTGCATCACAAAAAGATGCTTGTGGAAAACTCCTCGAAGTAAATACAGAAATTAAACCCGTTGATGTCAAAGGGAATAGAAGCAAGTCTGTATTGTTTGATGCATGTATTCTGGCAAAGGAGCTGAAGACTATGAAATGGAAATTGATAAGTGAAGTGTGGGTGGAATTACTATCATATGCTGCAAGTCATTGTAGACCAAGTAGCCATGCCCAATTGCTGAGTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTATTAATGGCTCATTTTGGCATAGCAGAGCAATTCCTGGCAGAGCAGTTCCAAGTGCTACCGGCCGAAGCAAGAGTAAAACTGATGGTTGAAAAGCCAGCGGATGAAGTCTTTTATTGGTCTGGTGAAAAGCCACTCGAGTCAAAAGCCACCGGCCCCGCCACCCACAACCACCACCGCCTCCCGTTTCCGCCACCACCTCCTCGCCCGCCAATTACTTCCTCCTTCCCGCGCCTAACGGCGTCGTCGGCGCTGCTCGGCTTCCACAATCCGATGCCTCATCTCCGGCGTCGGCGGCGGCGCGTGTCCGACGAGTTCGTCTCGACACCGAAGTCCGGCTTCGACCGCGGATTCTCCGTTATCGCCAATATGCTGAAGCGGATTGAGCCGCTTGACAGCTCCGTTATCTCCAAAGGCGTTTCCGATTCCGCTAAGGACTCGATGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

716

Amino Acids

81.62

Weight (kDa)

9.33

Isoelectric Point (pI)

52.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 3 - 282 5.5e-48 Domain of unknown function (DUF4220)
DUF594 PF04578 525 - 575 2.2e-23 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 2018, 2032
AccB1I GGYRCC 1 cut(s) 626
AccI GTMKAC 3 cut(s) 764, 824, 1642
AccII CGCG 5 cut(s) 440, 1141, 1933, 2004, 2049
AclWI GGATC 4 cut(s) 800, 957, 1034, 1450
AcoI YGGCCR 3 cut(s) 32, 694, 1764
AcsI RAATTY 1 cut(s) 224
AcuI CTGAAG 2 cut(s) 1592, 2096
AcvI CACGTG 1 cut(s) 86
AcyI GRCGYC 2 cut(s) 1943, 1990
AfaI GTAC 4 cut(s) 138, 167, 179, 1097
AfiI CCNNNNNNNGG 3 cut(s) 146, 1049, 2036
AflIII ACRYGT 1 cut(s) 83
AgeI ACCGGT 1 cut(s) 139
AgsI TTSAA 7 cut(s) 645, 748, 955, 1007, 1015, 1427, 1793
AjnI CCWGG 2 cut(s) 55, 1737
Alw21I GWGCWC 2 cut(s) 450, 1291
Alw26I GTCTC 2 cut(s) 119, 2026
AlwI GGATC 4 cut(s) 800, 957, 1034, 1450
Ama87I CYCGRG 2 cut(s) 12, 1838
AoxI GGCC 5 cut(s) 32, 694, 1115, 1764, 1855
ApeKI GCWGC 5 cut(s) 194, 1053, 1345, 1628, 1954
ApoI RAATTY 1 cut(s) 224
AseI ATTAAT 3 cut(s) 288, 434, 1706
AsiGI ACCGGT 1 cut(s) 139
AspLEI GCGC 5 cut(s) 1212, 1438, 1935, 1954, 2004
AspS9I GGNCC 3 cut(s) 149, 1115, 1856
AsuHPI GGTGA 4 cut(s) 319, 1259, 1283, 1838
AvaI CYCGRG 2 cut(s) 12, 1838
AvaII GGWCC 1 cut(s) 149
BaeGI GKGCMC 1 cut(s) 908
BalI TGGCCA 2 cut(s) 34, 696
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 2 cut(s) 450, 933
BbrPI CACGTG 1 cut(s) 86
BbsI GAAGAC 2 cut(s) 1353, 1580
Bbv12I GWGCWC 2 cut(s) 450, 1291
BbvI GCAGC 5 cut(s) 181, 1065, 1357, 1615, 1941
BccI CCATC 6 cut(s) 110, 347, 801, 1103, 1277, 1780
BceAI ACGGC 3 cut(s) 292, 375, 1956
BcgI CGANNNNNNTGC 1 cut(s) 27
BciT130I CCWGG 2 cut(s) 57, 1739
BclI TGATCA 1 cut(s) 1249
BcoDI GTCTC 2 cut(s) 119, 2026
BfmI CTRYAG 2 cut(s) 876, 1365
BfoI RGCGCY 2 cut(s) 1439, 1955
BglII AGATCT 2 cut(s) 355, 752
BisI GCNGC 8 cut(s) 195, 1054, 1346, 1629, 1955, 1997, 2000, 2090
BlsI GCNGC 8 cut(s) 196, 1055, 1347, 1630, 1956, 1998, 2001, 2091
BmcAI AGTACT 2 cut(s) 179, 1097
Bme1390I CCNGG 2 cut(s) 57, 1739
Bme18I GGWCC 1 cut(s) 149
BmeT110I CYCGRG 2 cut(s) 12, 1838
BmgT120I GGNCC 3 cut(s) 149, 1115, 1856
BmiI GGNNCC 2 cut(s) 628, 1858
BmrFI CCNGG 2 cut(s) 57, 1739
BmsI GCATC 6 cut(s) 648, 1021, 1453, 1460, 1537, 1965
BoxI GACNNNNGTC 1 cut(s) 372
BpiI GAAGAC 2 cut(s) 1353, 1580
BplI GAGNNNNNCTC 2 cut(s) 2006, 2038
Bpu10I CCTNAGC 1 cut(s) 2133
BpuEI CTTGAG 2 cut(s) 101, 917
BsaAI YACGTR 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 662
BsaHI GRCGYC 2 cut(s) 1943, 1990
BsaJI CCNNGG 5 cut(s) 55, 550, 636, 1102, 2047
BsaWI WCCGGW 2 cut(s) 139, 262
BsaXI ACNNNNNCTCC 2 cut(s) 1671, 1701
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 1049, 2036
Bse118I RCCGGY 3 cut(s) 139, 1762, 1853
Bse1I ACTGG 1 cut(s) 206
Bse3DI GCAATG 3 cut(s) 1194, 1246, 1270
Bse8I GATNNNNATC 1 cut(s) 662
BseBI CCWGG 2 cut(s) 57, 1739
BseDI CCNNGG 5 cut(s) 55, 550, 636, 1102, 2047
BseGI GGATG 4 cut(s) 178, 1036, 1114, 1810
BseJI GATNNNNATC 1 cut(s) 662
BseLI CCNNNNNNNGG 3 cut(s) 146, 1049, 2036
BseMI GCAATG 3 cut(s) 1194, 1246, 1270
BseMII CTCAG 3 cut(s) 972, 1244, 1658
BseNI ACTGG 1 cut(s) 206
BseRI GAGGAG 2 cut(s) 1469, 1893
BseSI GKGCMC 1 cut(s) 908
BseX3I CGGCCG 1 cut(s) 1764
BseXI GCAGC 5 cut(s) 181, 1065, 1357, 1615, 1941
BseYI CCCAGC 1 cut(s) 1438
Bsh1236I CGCG 5 cut(s) 440, 1141, 1933, 2004, 2049
Bsh1285I CGRYCG 2 cut(s) 1767, 2047
BshFI GGCC 5 cut(s) 34, 696, 1117, 1766, 1857
BshNI GGYRCC 1 cut(s) 626
BshTI ACCGGT 1 cut(s) 139
BsiEI CGRYCG 2 cut(s) 1767, 2047
BsiHKAI GWGCWC 2 cut(s) 450, 1291
BsiHKCI CYCGRG 2 cut(s) 12, 1838
BsiSI CCGG 6 cut(s) 140, 263, 1763, 1854, 1987, 2037
BslFI GGGAC 2 cut(s) 221, 395
BslI CCNNNNNNNGG 3 cut(s) 146, 1049, 2036
BsmAI GTCTC 2 cut(s) 119, 2026
BsmBI CGTCTC 1 cut(s) 2026
BsmFI GGGAC 2 cut(s) 221, 395
BsmI GAATGC 3 cut(s) 493, 863, 1179
BsnI GGCC 5 cut(s) 34, 696, 1117, 1766, 1857
BsoBI CYCGRG 2 cut(s) 12, 1838
Bsp1286I GDGCHC 4 cut(s) 450, 908, 933, 1291
Bsp143I GATC 9 cut(s) 355, 752, 792, 962, 1039, 1136, 1171, 1249, 1442
Bsp19I CCATGG 1 cut(s) 1102
Bsp68I TCGCGA 2 cut(s) 440, 1141
BspANI GGCC 5 cut(s) 34, 696, 1117, 1766, 1857
BspCNI CTCAG 3 cut(s) 973, 1243, 1659
BspFNI CGCG 5 cut(s) 440, 1141, 1933, 2004, 2049
BspLI GGNNCC 2 cut(s) 628, 1858
BspPI GGATC 4 cut(s) 800, 957, 1034, 1450
BspT107I GGYRCC 1 cut(s) 626
BsrDI GCAATG 3 cut(s) 1194, 1246, 1270
BsrFI RCCGGY 3 cut(s) 139, 1762, 1853
BsrI ACTGG 1 cut(s) 206
BssAI RCCGGY 3 cut(s) 139, 1762, 1853
BssECI CCNNGG 5 cut(s) 55, 550, 636, 1102, 2047
BssMI GATC 9 cut(s) 355, 752, 792, 962, 1039, 1136, 1171, 1249, 1442
BssNI GRCGYC 2 cut(s) 1943, 1990
BssT1I CCWWGG 3 cut(s) 550, 636, 1102
Bst2UI CCWGG 2 cut(s) 57, 1739
Bst4CI ACNGT 5 cut(s) 538, 682, 880, 990, 1122
Bst6I CTCTTC 3 cut(s) 743, 859, 1002
BstACI GRCGYC 2 cut(s) 1943, 1990
BstBAI YACGTR 1 cut(s) 86
BstC8I GCNNGC 4 cut(s) 77, 1115, 1800, 1910
BstDEI CTNAG 6 cut(s) 981, 1088, 1230, 1336, 1667, 2133
BstDSI CCRYGG 2 cut(s) 1102, 2047
BstF5I GGATG 4 cut(s) 178, 1036, 1114, 1810
BstFNI CGCG 5 cut(s) 440, 1141, 1933, 2004, 2049
BstH2I RGCGCY 2 cut(s) 1439, 1955
BstHHI GCGC 5 cut(s) 1212, 1438, 1935, 1954, 2004
BstKTI GATC 9 cut(s) 358, 755, 795, 965, 1042, 1139, 1174, 1252, 1445
BstMAI GTCTC 2 cut(s) 119, 2026
BstMBI GATC 9 cut(s) 355, 752, 792, 962, 1039, 1136, 1171, 1249, 1442
BstMCI CGRYCG 2 cut(s) 1767, 2047
BstMWI GCNNNNNNNGC 8 cut(s) 31, 191, 499, 903, 1949, 1996, 2046, 2086
BstNI CCWGG 2 cut(s) 57, 1739
BstNSI RCATGY 1 cut(s) 1554
BstPAI GACNNNNGTC 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 55, 1737
BstSFI CTRYAG 2 cut(s) 876, 1365
BstSLI GKGCMC 1 cut(s) 908
BstUI CGCG 5 cut(s) 440, 1141, 1933, 2004, 2049
BstV1I GCAGC 5 cut(s) 181, 1065, 1357, 1615, 1941
BstV2I GAAGAC 2 cut(s) 1353, 1580
BstX2I RGATCY 4 cut(s) 355, 752, 792, 962
BstXI CCANNNNNNTGG 2 cut(s) 637, 1109
BstYI RGATCY 4 cut(s) 355, 752, 792, 962
BstZI CGGCCG 1 cut(s) 1764
BsuRI GGCC 5 cut(s) 34, 696, 1117, 1766, 1857
BtgI CCRYGG 2 cut(s) 1102, 2047
BtgZI GCGATG 2 cut(s) 325, 1170
BtsCI GGATG 4 cut(s) 178, 1036, 1114, 1810
BtsIMutI CAGTG 1 cut(s) 1118
BtuMI TCGCGA 2 cut(s) 440, 1141
Cac8I GCNNGC 4 cut(s) 77, 1115, 1800, 1910
CfoI GCGC 5 cut(s) 1212, 1438, 1935, 1954, 2004
Cfr10I RCCGGY 3 cut(s) 139, 1762, 1853
Cfr13I GGNCC 3 cut(s) 149, 1115, 1856
Cfr42I CCGCGG 1 cut(s) 2050
CseI GACGC 2 cut(s) 1932, 1979
Csp6I GTAC 4 cut(s) 137, 166, 178, 1096
CspAI ACCGGT 1 cut(s) 139
CviAII CATG 6 cut(s) 169, 727, 925, 1103, 1551, 1655
CviQI GTAC 4 cut(s) 137, 166, 178, 1096
DdeI CTNAG 6 cut(s) 981, 1088, 1230, 1336, 1667, 2133
DpnI GATC 9 cut(s) 357, 754, 794, 964, 1041, 1138, 1173, 1251, 1444
DpnII GATC 9 cut(s) 355, 752, 792, 962, 1039, 1136, 1171, 1249, 1442
DraI TTTAAA 1 cut(s) 331
DrdI GACNNNNNNGTC 2 cut(s) 2018, 2032
DseDI GACNNNNNNGTC 2 cut(s) 2018, 2032
EaeI YGGCCR 3 cut(s) 32, 694, 1764
EagI CGGCCG 1 cut(s) 1764
Eam1104I CTCTTC 3 cut(s) 743, 859, 1002
EarI CTCTTC 3 cut(s) 743, 859, 1002
EciI GGCGGA 1 cut(s) 1881
Ecl136II GAGCTC 1 cut(s) 448
EclXI CGGCCG 1 cut(s) 1764
Eco130I CCWWGG 3 cut(s) 550, 636, 1102
Eco24I GRGCYC 2 cut(s) 450, 933
Eco47I GGWCC 1 cut(s) 149
Eco52I CGGCCG 1 cut(s) 1764
Eco53kI GAGCTC 1 cut(s) 448
Eco57I CTGAAG 2 cut(s) 1592, 2096
Eco72I CACGTG 1 cut(s) 86
Eco88I CYCGRG 2 cut(s) 12, 1838
EcoICRI GAGCTC 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 224
EcoRII CCWGG 2 cut(s) 55, 1737
EcoT14I CCWWGG 3 cut(s) 550, 636, 1102
EcoT22I ATGCAT 1 cut(s) 1552
EcoT38I GRGCYC 2 cut(s) 450, 933
ErhI CCWWGG 3 cut(s) 550, 636, 1102
Esp3I CGTCTC 1 cut(s) 2026
FaeI CATG 6 cut(s) 172, 730, 928, 1106, 1554, 1658
FalI AAGNNNNNCTT 2 cut(s) 2024, 2056
FaqI GGGAC 2 cut(s) 221, 395
FatI CATG 6 cut(s) 168, 726, 924, 1102, 1550, 1654
FauI CCCGC 8 cut(s) 372, 495, 974, 1106, 1363, 1867, 1917, 1938
FauNDI CATATG 1 cut(s) 1624
FbaI TGATCA 1 cut(s) 1249
FblI GTMKAC 3 cut(s) 764, 824, 1642
Fnu4HI GCNGC 8 cut(s) 195, 1054, 1346, 1629, 1955, 1997, 2000, 2090
FokI GGATG 4 cut(s) 185, 1043, 1121, 1817
FriOI GRGCYC 2 cut(s) 450, 933
Fsp4HI GCNGC 8 cut(s) 195, 1054, 1346, 1629, 1955, 1997, 2000, 2090
GlaI GCGC 5 cut(s) 1211, 1437, 1934, 1953, 2003
GluI GCNGC 8 cut(s) 195, 1054, 1346, 1629, 1955, 1997, 2000, 2090
GsaI CCCAGC 1 cut(s) 1442
HaeII RGCGCY 2 cut(s) 1439, 1955
HaeIII GGCC 5 cut(s) 34, 696, 1117, 1766, 1857
HapII CCGG 6 cut(s) 140, 263, 1763, 1854, 1987, 2037
HgaI GACGC 2 cut(s) 1932, 1979
HhaI GCGC 5 cut(s) 1212, 1438, 1935, 1954, 2004
Hin1I GRCGYC 2 cut(s) 1943, 1990
Hin1II CATG 6 cut(s) 172, 730, 928, 1106, 1554, 1658
Hin6I GCGC 5 cut(s) 1210, 1436, 1933, 1952, 2002
HinP1I GCGC 5 cut(s) 1210, 1436, 1933, 1952, 2002
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HindIII AAGCTT 1 cut(s) 480
HpaII CCGG 6 cut(s) 140, 263, 1763, 1854, 1987, 2037
HphI GGTGA 4 cut(s) 319, 1259, 1283, 1838
Hpy166II GTNNAC 4 cut(s) 9, 765, 825, 1643
Hpy188III TCNNGA 6 cut(s) 14, 439, 944, 1140, 1295, 2023
Hpy8I GTNNAC 4 cut(s) 9, 765, 825, 1643
Hpy99I CGWCG 4 cut(s) 1948, 1951, 1995, 2015
HpyAV CCTTC 1 cut(s) 1936
HpyCH4III ACNGT 5 cut(s) 538, 682, 880, 990, 1122
HpyCH4IV ACGT 2 cut(s) 85, 313
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 191, 499, 903, 1949, 1996, 2046, 2086
HpyF3I CTNAG 6 cut(s) 981, 1088, 1230, 1336, 1667, 2133
HpySE526I ACGT 2 cut(s) 85, 313
Hsp92I GRCGYC 2 cut(s) 1943, 1990
Hsp92II CATG 6 cut(s) 172, 730, 928, 1106, 1554, 1658
HspAI GCGC 5 cut(s) 1210, 1436, 1933, 1952, 2002
Ksp22I TGATCA 1 cut(s) 1249
KspI CCGCGG 1 cut(s) 2050
Kzo9I GATC 9 cut(s) 355, 752, 792, 962, 1039, 1136, 1171, 1249, 1442
LmnI GCTCC 2 cut(s) 1567, 2105
Lsp1109I GCAGC 5 cut(s) 181, 1065, 1357, 1615, 1941
LweI GCATC 6 cut(s) 648, 1021, 1453, 1460, 1537, 1965
MaeII ACGT 2 cut(s) 85, 313
MaeIII GTNAC 4 cut(s) 410, 604, 1162, 1687
MalI GATC 9 cut(s) 357, 754, 794, 964, 1041, 1138, 1173, 1251, 1444
MboI GATC 9 cut(s) 355, 752, 792, 962, 1039, 1136, 1171, 1249, 1442
MfeI CAATTG 1 cut(s) 1661
MflI RGATCY 4 cut(s) 355, 752, 792, 962
MhlI GDGCHC 4 cut(s) 450, 908, 933, 1291
MlsI TGGCCA 2 cut(s) 34, 696
MluNI TGGCCA 2 cut(s) 34, 696
MlyI GAGTC 4 cut(s) 116, 1088, 1850, 2132
MmeI TCCRAC 1 cut(s) 2034
Mox20I TGGCCA 2 cut(s) 34, 696
Mph1103I ATGCAT 1 cut(s) 1552
MscI TGGCCA 2 cut(s) 34, 696
MseI TTAA 9 cut(s) 288, 330, 434, 515, 720, 1125, 1133, 1500, 1706
MslI CAYNNNNRTG 1 cut(s) 1107
Msp20I TGGCCA 2 cut(s) 34, 696
MspA1I CMGCKG 3 cut(s) 379, 1802, 2049
MspI CCGG 6 cut(s) 140, 263, 1763, 1854, 1987, 2037
MspR9I CCNGG 2 cut(s) 57, 1739
MunI CAATTG 1 cut(s) 1661
Mva1269I GAATGC 3 cut(s) 493, 863, 1179
MvaI CCWGG 2 cut(s) 57, 1739
MvnI CGCG 5 cut(s) 440, 1141, 1933, 2004, 2049
MwoI GCNNNNNNNGC 8 cut(s) 31, 191, 499, 903, 1949, 1996, 2046, 2086
NcoI CCATGG 1 cut(s) 1102
NdeI CATATG 1 cut(s) 1624
NdeII GATC 9 cut(s) 355, 752, 792, 962, 1039, 1136, 1171, 1249, 1442
NlaIII CATG 6 cut(s) 172, 730, 928, 1106, 1554, 1658
NlaIV GGNNCC 2 cut(s) 628, 1858
NmeAIII GCCGAG 2 cut(s) 478, 1938
NmuCI GTSAC 1 cut(s) 1687
NruI TCGCGA 2 cut(s) 440, 1141
NsiI ATGCAT 1 cut(s) 1552
NspI RCATGY 1 cut(s) 1554
PaeR7I CTCGAG 2 cut(s) 12, 1838
PctI GAATGC 3 cut(s) 493, 863, 1179
PfeI GAWTC 7 cut(s) 242, 641, 815, 921, 1084, 2052, 2126
PflFI GACNNNGTC 1 cut(s) 1312
PinAI ACCGGT 1 cut(s) 139
PkrI GCNGC 8 cut(s) 196, 1055, 1347, 1630, 1956, 1998, 2001, 2091
PleI GAGTC 4 cut(s) 116, 1087, 1849, 2132
PmaCI CACGTG 1 cut(s) 86
PmlI CACGTG 1 cut(s) 86
PpsI GAGTC 4 cut(s) 116, 1087, 1849, 2132
Ppu21I YACGTR 1 cut(s) 86
PshAI GACNNNNGTC 1 cut(s) 372
PshBI ATTAAT 3 cut(s) 288, 434, 1706
Psp124BI GAGCTC 1 cut(s) 450
Psp6I CCWGG 2 cut(s) 55, 1737
PspCI CACGTG 1 cut(s) 86
PspFI CCCAGC 1 cut(s) 1438
PspGI CCWGG 2 cut(s) 55, 1737
PspN4I GGNNCC 2 cut(s) 628, 1858
PspPI GGNCC 3 cut(s) 149, 1115, 1856
PspXI VCTCGAGB 1 cut(s) 1838
PsuI RGATCY 4 cut(s) 355, 752, 792, 962
PsyI GACNNNGTC 1 cut(s) 1312
RruI TCGCGA 2 cut(s) 440, 1141
RsaI GTAC 4 cut(s) 138, 167, 179, 1097
RsaNI GTAC 4 cut(s) 137, 166, 178, 1096
RseI CAYNNNNRTG 1 cut(s) 1107
SacI GAGCTC 1 cut(s) 450
SacII CCGCGG 1 cut(s) 2050
SaqAI TTAA 9 cut(s) 288, 330, 434, 515, 720, 1125, 1133, 1500, 1706
SatI GCNGC 8 cut(s) 195, 1054, 1346, 1629, 1955, 1997, 2000, 2090
Sau3AI GATC 9 cut(s) 355, 752, 792, 962, 1039, 1136, 1171, 1249, 1442
Sau96I GGNCC 3 cut(s) 149, 1115, 1856
ScaI AGTACT 2 cut(s) 179, 1097
SchI GAGTC 4 cut(s) 116, 1088, 1850, 2132
ScrFI CCNGG 2 cut(s) 57, 1739
SduI GDGCHC 4 cut(s) 450, 908, 933, 1291
SfaNI GCATC 6 cut(s) 648, 1021, 1453, 1460, 1537, 1965
SfcI CTRYAG 2 cut(s) 876, 1365
Sfr274I CTCGAG 2 cut(s) 12, 1838
Sfr303I CCGCGG 1 cut(s) 2050
SgrBI CCGCGG 1 cut(s) 2050
SinI GGWCC 1 cut(s) 149
SlaI CTCGAG 2 cut(s) 12, 1838
SmiMI CAYNNNNRTG 1 cut(s) 1107
SmlI CTYRAG 4 cut(s) 12, 116, 932, 1838
SmoI CTYRAG 4 cut(s) 12, 116, 932, 1838
SspI AATATT 1 cut(s) 298
SstI GAGCTC 1 cut(s) 450
StyD4I CCNGG 2 cut(s) 55, 1737
StyI CCWWGG 3 cut(s) 550, 636, 1102
TaaI ACNGT 5 cut(s) 538, 682, 880, 990, 1122
TaiI ACGT 2 cut(s) 88, 316
TaqII GACCGA 1 cut(s) 166
TatI WGTACW 3 cut(s) 165, 177, 1095
TauI GCSGC 3 cut(s) 1999, 2002, 2092
TfiI GAWTC 7 cut(s) 242, 641, 815, 921, 1084, 2052, 2126
Tru1I TTAA 9 cut(s) 288, 330, 434, 515, 720, 1125, 1133, 1500, 1706
Tru9I TTAA 9 cut(s) 288, 330, 434, 515, 720, 1125, 1133, 1500, 1706
TscAI CASTG 1 cut(s) 1125
TseFI GTSAC 1 cut(s) 1687
TseI GCWGC 5 cut(s) 194, 1053, 1345, 1628, 1954
Tsp45I GTSAC 1 cut(s) 1687
TspDTI ATGAA 5 cut(s) 200, 477, 988, 1595, 1821
TspGWI ACGGA 2 cut(s) 2047, 2092
TspRI CASTG 1 cut(s) 1125
Tth111I GACNNNGTC 1 cut(s) 1312
VpaK11BI GGWCC 1 cut(s) 149
VspI ATTAAT 3 cut(s) 288, 434, 1706
XapI RAATTY 1 cut(s) 224
XceI RCATGY 1 cut(s) 1554
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XhoI CTCGAG 2 cut(s) 12, 1838
XmiI GTMKAC 3 cut(s) 764, 824, 1642
ZrmI AGTACT 2 cut(s) 179, 1097
Zsp2I ATGCAT 1 cut(s) 1552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.