Rh2BG250500

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000662
Physical Location & Seq
Reverse (-)
25667926 .. 25677053
9128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG250500.1

Sequence Viewer

Length: 1218 bp
ATGGCTTCATTAGCCGCTCCTATTTCTAACAAGGTCAAGAAGATCTGGGATAAGTGGAATCTGCGGAGCTTCATCATATTAAGTCTCTCATTGCAAACCTTCTTAATTCTCTTTGCACCCTTGAGAAAGAGAACATCGAGCAAATGGGTAATCGTGCCTATCTGGTTAGCTTACTTGCTCGCTGACTGGGCTGCTAACTTTGCTATCGGACTAATTGCAAGCAGCCAAACGAACACTGATCAAGGTTCCCCAGAGAATGGTAATCTCTTGACATTCTGGGCTCCCTTTCTTCTTTTGCACCTTGGTGGTCCAGACACCATAATTGCAATTGCTATGGAAGACAATGCGTTGTGGCCAAGGCACTTGCTTGGTCTAATATTCCAGGTTGTAGCTGCAATGTACGTCTTCGGTATGCAGTTCTCTAACCAAAACAAGCTACTGTGGCCAACACTCTTGCTGTTTGTACCAGGACTTGTCAAGTACGGCGAGCGAACGCGTGCTCTATATCTTGCCAGCTTGGAAAGTCTCAAGGAAACCATACTTGCAATACCGGAACCTGGACCGGATTATGCAAAGTACATGGATGAGTACTCCTCAAGAAAAGCTGCCGGTCTTCCAATTACAATAATAAAAGAAGGAGGTAGCTCAGAGTCCAAGAATGTGACTTACCGGCTGGGAGAAGGAAAAATTGATAATATTACACTAGTGGTGGGTGCTCGTCACCTGTTTCAAATGTCCATGGGTCTTATTATAGATCTTGTGTTCGATTATCATCAGCGCAATGAAAGCCGTGCCTATTTCAATAAATTAGAATCAGGGGATGCTTTTACATCGATTGCGACTGAGCTCAATTTTATGTATGAAGCTCTGTACACCAAAGCTTATGTGGTACATTCTCGATGCGGATACTTGTTGAGAGCTACATCCTTCATCGCAGTTTGCATAGCCTTTGTTTTCTTCTATAGATTAGAGAAACATGGCTTTTACAGGTTTGATGTTGGAATTACTTATTCCTTGCTTTTTGGTGCCATAGCTCTTGATGCAATAGCTCTTTCAATGCTGATATTATCTGATTGGACGATCGCTGTTGTATACAACTTCTGTAAAGAGTTTTCTGTAGTCACTGATCTCATGAAGAAGTACCTGGAGTTTAAGAGATCATGGCGACCAACAACTACATCAAAGTGTCTGGAGCGGACCAGAAGGATTTTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

405

Amino Acids

46.01

Weight (kDa)

9.19

Isoelectric Point (pI)

34.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 381 4.8e-87 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1025
AccB7I CCANNNNNTGG 1 cut(s) 257
AccBSI CCGCTC 2 cut(s) 17, 1195
AccI GTMKAC 1 cut(s) 1092
AccII CGCG 1 cut(s) 496
AciI CCGC 4 cut(s) 15, 64, 903, 1195
AcoI YGGCCR 2 cut(s) 353, 443
AfaI GTAC 8 cut(s) 401, 465, 482, 578, 590, 872, 891, 1142
AfiI CCNNNNNNNGG 2 cut(s) 257, 557
AflIII ACRYGT 1 cut(s) 494
AgsI TTSAA 3 cut(s) 731, 802, 1056
AhlI ACTAGT 1 cut(s) 703
AjnI CCWGG 4 cut(s) 381, 466, 556, 1143
AleI CACNNNNGTG 1 cut(s) 303
Alw21I GWGCWC 3 cut(s) 502, 718, 849
Alw26I GTCTC 2 cut(s) 89, 530
AoxI GGCC 2 cut(s) 353, 443
ApeKI GCWGC 4 cut(s) 191, 222, 392, 605
AspLEI GCGC 1 cut(s) 780
AspS9I GGNCC 3 cut(s) 308, 560, 1197
AsuHPI GGTGA 1 cut(s) 713
AvaII GGWCC 3 cut(s) 308, 560, 1197
BalI TGGCCA 2 cut(s) 355, 445
BanI GGYRCC 1 cut(s) 1025
BanII GRGCYC 2 cut(s) 283, 849
BbsI GAAGAC 3 cut(s) 345, 397, 605
Bbv12I GWGCWC 3 cut(s) 502, 718, 849
BbvI GCAGC 4 cut(s) 178, 234, 379, 592
BceAI ACGGC 2 cut(s) 499, 774
BciT130I CCWGG 4 cut(s) 383, 468, 558, 1145
BciVI GTATCC 1 cut(s) 899
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 2 cut(s) 89, 530
BcuI ACTAGT 1 cut(s) 703
BfaI CTAG 1 cut(s) 704
BfmI CTRYAG 2 cut(s) 961, 1116
BfuI GTATCC 1 cut(s) 899
BglII AGATCT 2 cut(s) 42, 754
BisI GCNGC 5 cut(s) 15, 192, 223, 393, 606
BlsI GCNGC 5 cut(s) 16, 193, 224, 394, 607
BmcAI AGTACT 1 cut(s) 590
Bme1390I CCNGG 4 cut(s) 383, 468, 558, 1145
Bme18I GGWCC 3 cut(s) 308, 560, 1197
BmgT120I GGNCC 3 cut(s) 308, 560, 1197
BmiI GGNNCC 4 cut(s) 247, 282, 555, 1027
BmrFI CCNGG 4 cut(s) 383, 468, 558, 1145
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 3 cut(s) 811, 890, 1030
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 3 cut(s) 345, 397, 605
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 2 cut(s) 1166, 1211
BpuEI CTTGAG 3 cut(s) 142, 512, 580
Bsa29I ATCGAT 1 cut(s) 833
BsaBI GATNNNNATC 1 cut(s) 771
BsaJI CCNNGG 3 cut(s) 301, 356, 738
BsaWI WCCGGW 2 cut(s) 550, 562
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 557
Bse118I RCCGGY 2 cut(s) 608, 669
Bse1I ACTGG 1 cut(s) 191
Bse3DI GCAATG 3 cut(s) 89, 402, 787
Bse8I GATNNNNATC 1 cut(s) 771
BseBI CCWGG 4 cut(s) 383, 468, 558, 1145
BseCI ATCGAT 1 cut(s) 833
BseDI CCNNGG 3 cut(s) 301, 356, 738
BseGI GGATG 3 cut(s) 589, 826, 923
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 2 cut(s) 257, 557
BseMI GCAATG 3 cut(s) 89, 402, 787
BseMII CTCAG 2 cut(s) 660, 834
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 1 cut(s) 583
BseXI GCAGC 4 cut(s) 178, 234, 379, 592
BseYI CCCAGC 1 cut(s) 673
Bsh1236I CGCG 1 cut(s) 496
Bsh1285I CGRYCG 1 cut(s) 1083
BshFI GGCC 2 cut(s) 355, 445
BshNI GGYRCC 1 cut(s) 1025
BshVI ATCGAT 1 cut(s) 833
BsiEI CGRYCG 1 cut(s) 1083
BsiHKAI GWGCWC 3 cut(s) 502, 718, 849
BsiSI CCGG 4 cut(s) 551, 563, 609, 670
BslI CCNNNNNNNGG 2 cut(s) 257, 557
BsmAI GTCTC 2 cut(s) 89, 530
BsnI GGCC 2 cut(s) 355, 445
Bsp1286I GDGCHC 4 cut(s) 283, 502, 718, 849
Bsp1407I TGTACA 1 cut(s) 870
Bsp143I GATC 6 cut(s) 42, 238, 754, 1080, 1126, 1157
Bsp19I CCATGG 1 cut(s) 738
BspACI CCGC 4 cut(s) 15, 64, 903, 1195
BspANI GGCC 2 cut(s) 355, 445
BspCNI CTCAG 2 cut(s) 659, 835
BspDI ATCGAT 1 cut(s) 833
BspFNI CGCG 1 cut(s) 496
BspHI TCATGA 1 cut(s) 1131
BspLI GGNNCC 4 cut(s) 247, 282, 555, 1027
BspT107I GGYRCC 1 cut(s) 1025
BsrBI CCGCTC 2 cut(s) 17, 1195
BsrDI GCAATG 3 cut(s) 89, 402, 787
BsrFI RCCGGY 2 cut(s) 608, 669
BsrGI TGTACA 1 cut(s) 870
BsrI ACTGG 1 cut(s) 191
BssAI RCCGGY 2 cut(s) 608, 669
BssECI CCNNGG 3 cut(s) 301, 356, 738
BssMI GATC 6 cut(s) 42, 238, 754, 1080, 1126, 1157
BssNAI GTATAC 1 cut(s) 1093
BssT1I CCWWGG 3 cut(s) 301, 356, 738
Bst1107I GTATAC 1 cut(s) 1093
Bst2UI CCWGG 4 cut(s) 383, 468, 558, 1145
Bst4CI ACNGT 1 cut(s) 441
BstAUI TGTACA 1 cut(s) 870
BstC8I GCNNGC 5 cut(s) 180, 220, 488, 498, 514
BstDEI CTNAG 2 cut(s) 646, 843
BstDSI CCRYGG 1 cut(s) 738
BstF5I GGATG 3 cut(s) 589, 826, 923
BstFNI CGCG 1 cut(s) 496
BstHHI GCGC 1 cut(s) 780
BstKTI GATC 6 cut(s) 45, 241, 757, 1083, 1129, 1160
BstMAI GTCTC 2 cut(s) 89, 530
BstMBI GATC 6 cut(s) 42, 238, 754, 1080, 1126, 1157
BstMCI CGRYCG 1 cut(s) 1083
BstMWI GCNNNNNNNGC 6 cut(s) 11, 188, 200, 442, 786, 1040
BstNI CCWGG 4 cut(s) 383, 468, 558, 1145
BstSCI CCNGG 4 cut(s) 381, 466, 556, 1143
BstSFI CTRYAG 2 cut(s) 961, 1116
BstUI CGCG 1 cut(s) 496
BstV1I GCAGC 4 cut(s) 178, 234, 379, 592
BstV2I GAAGAC 3 cut(s) 345, 397, 605
BstX2I RGATCY 2 cut(s) 42, 754
BstYI RGATCY 2 cut(s) 42, 754
BstZ17I GTATAC 1 cut(s) 1093
Bsu15I ATCGAT 1 cut(s) 833
BsuI GTATCC 1 cut(s) 899
BsuRI GGCC 2 cut(s) 355, 445
BsuTUI ATCGAT 1 cut(s) 833
BtgI CCRYGG 1 cut(s) 738
BtgZI GCGATG 1 cut(s) 916
BtsCI GGATG 3 cut(s) 589, 826, 923
BtsIMutI CAGTG 2 cut(s) 234, 1122
Cac8I GCNNGC 5 cut(s) 180, 220, 488, 498, 514
CciI TCATGA 1 cut(s) 1131
CfoI GCGC 1 cut(s) 780
Cfr10I RCCGGY 2 cut(s) 608, 669
Cfr13I GGNCC 3 cut(s) 308, 560, 1197
ClaI ATCGAT 1 cut(s) 833
Csp6I GTAC 8 cut(s) 400, 464, 481, 577, 589, 871, 890, 1141
CviAII CATG 5 cut(s) 580, 739, 977, 1132, 1161
CviQI GTAC 8 cut(s) 400, 464, 481, 577, 589, 871, 890, 1141
DdeI CTNAG 2 cut(s) 646, 843
DpnI GATC 6 cut(s) 44, 240, 756, 1082, 1128, 1159
DpnII GATC 6 cut(s) 42, 238, 754, 1080, 1126, 1157
EaeI YGGCCR 2 cut(s) 353, 443
Ecl136II GAGCTC 1 cut(s) 847
Eco130I CCWWGG 3 cut(s) 301, 356, 738
Eco24I GRGCYC 2 cut(s) 283, 849
Eco47I GGWCC 3 cut(s) 308, 560, 1197
Eco53kI GAGCTC 1 cut(s) 847
EcoICRI GAGCTC 1 cut(s) 847
EcoRII CCWGG 4 cut(s) 381, 466, 556, 1143
EcoT14I CCWWGG 3 cut(s) 301, 356, 738
EcoT38I GRGCYC 2 cut(s) 283, 849
ErhI CCWWGG 3 cut(s) 301, 356, 738
FaeI CATG 5 cut(s) 583, 742, 980, 1135, 1164
FatI CATG 5 cut(s) 579, 738, 976, 1131, 1160
FbaI TGATCA 1 cut(s) 238
FblI GTMKAC 1 cut(s) 1092
Fnu4HI GCNGC 5 cut(s) 15, 192, 223, 393, 606
FokI GGATG 3 cut(s) 596, 833, 910
FriOI GRGCYC 2 cut(s) 283, 849
Fsp4HI GCNGC 5 cut(s) 15, 192, 223, 393, 606
FspBI CTAG 1 cut(s) 704
GlaI GCGC 1 cut(s) 779
GluI GCNGC 5 cut(s) 15, 192, 223, 393, 606
GsaI CCCAGC 1 cut(s) 677
GsuI CTGGAG 2 cut(s) 1166, 1211
HaeIII GGCC 2 cut(s) 355, 445
HapII CCGG 4 cut(s) 551, 563, 609, 670
HhaI GCGC 1 cut(s) 780
Hin1II CATG 5 cut(s) 583, 742, 980, 1135, 1164
Hin6I GCGC 1 cut(s) 778
HinP1I GCGC 1 cut(s) 778
HindIII AAGCTT 1 cut(s) 879
HinfI GANTC 3 cut(s) 58, 650, 812
HpaII CCGG 4 cut(s) 551, 563, 609, 670
HphI GGTGA 1 cut(s) 713
Hpy166II GTNNAC 2 cut(s) 873, 1093
Hpy188I TCNGA 3 cut(s) 209, 649, 1072
Hpy188III TCNNGA 8 cut(s) 37, 268, 311, 597, 897, 1037, 1132, 1190
Hpy8I GTNNAC 2 cut(s) 873, 1093
HpyAV CCTTC 5 cut(s) 109, 629, 674, 937, 1197
HpyCH4III ACNGT 1 cut(s) 441
HpyCH4IV ACGT 1 cut(s) 402
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 188, 200, 442, 786, 1040
HpyF3I CTNAG 2 cut(s) 646, 843
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 5 cut(s) 583, 742, 980, 1135, 1164
HspAI GCGC 1 cut(s) 778
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 6 cut(s) 42, 238, 754, 1080, 1126, 1157
LmnI GCTCC 4 cut(s) 22, 66, 286, 1192
Lsp1109I GCAGC 4 cut(s) 178, 234, 379, 592
LweI GCATC 3 cut(s) 811, 890, 1030
MaeI CTAG 1 cut(s) 704
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 3 cut(s) 661, 719, 1120
MalI GATC 6 cut(s) 44, 240, 756, 1082, 1128, 1159
MbiI CCGCTC 2 cut(s) 17, 1195
MboI GATC 6 cut(s) 42, 238, 754, 1080, 1126, 1157
MboII GAAGA 7 cut(s) 52, 281, 350, 397, 605, 949, 1147
MfeI CAATTG 1 cut(s) 327
MflI RGATCY 2 cut(s) 42, 754
MhlI GDGCHC 4 cut(s) 283, 502, 718, 849
MlsI TGGCCA 2 cut(s) 355, 445
MluCI AATT 9 cut(s) 105, 213, 321, 327, 618, 687, 806, 850, 1002
MluI ACGCGT 1 cut(s) 494
MluNI TGGCCA 2 cut(s) 355, 445
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 1 cut(s) 979
MnlI CCTC 2 cut(s) 604, 632
Mox20I TGGCCA 2 cut(s) 355, 445
MscI TGGCCA 2 cut(s) 355, 445
MseI TTAA 3 cut(s) 80, 104, 1152
MslI CAYNNNNRTG 2 cut(s) 303, 1183
Msp20I TGGCCA 2 cut(s) 355, 445
MspI CCGG 4 cut(s) 551, 563, 609, 670
MspR9I CCNGG 4 cut(s) 383, 468, 558, 1145
MunI CAATTG 1 cut(s) 327
MvaI CCWGG 4 cut(s) 383, 468, 558, 1145
MvnI CGCG 1 cut(s) 496
MwoI GCNNNNNNNGC 6 cut(s) 11, 188, 200, 442, 786, 1040
NcoI CCATGG 1 cut(s) 738
NdeII GATC 6 cut(s) 42, 238, 754, 1080, 1126, 1157
NlaIII CATG 5 cut(s) 583, 742, 980, 1135, 1164
NlaIV GGNNCC 4 cut(s) 247, 282, 555, 1027
NmuCI GTSAC 3 cut(s) 661, 719, 1120
OliI CACNNNNGTG 1 cut(s) 303
PagI TCATGA 1 cut(s) 1131
PfeI GAWTC 2 cut(s) 58, 812
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 5 cut(s) 16, 193, 224, 394, 607
Ple19I CGATCG 1 cut(s) 1083
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
Psp124BI GAGCTC 1 cut(s) 849
Psp6I CCWGG 4 cut(s) 381, 466, 556, 1143
PspFI CCCAGC 1 cut(s) 673
PspGI CCWGG 4 cut(s) 381, 466, 556, 1143
PspN4I GGNNCC 4 cut(s) 247, 282, 555, 1027
PspPI GGNCC 3 cut(s) 308, 560, 1197
PsuI RGATCY 2 cut(s) 42, 754
PvuI CGATCG 1 cut(s) 1083
RsaI GTAC 8 cut(s) 401, 465, 482, 578, 590, 872, 891, 1142
RsaNI GTAC 8 cut(s) 400, 464, 481, 577, 589, 871, 890, 1141
RseI CAYNNNNRTG 2 cut(s) 303, 1183
SacI GAGCTC 1 cut(s) 849
SaqAI TTAA 3 cut(s) 80, 104, 1152
SatI GCNGC 5 cut(s) 15, 192, 223, 393, 606
Sau3AI GATC 6 cut(s) 42, 238, 754, 1080, 1126, 1157
Sau96I GGNCC 3 cut(s) 308, 560, 1197
ScaI AGTACT 1 cut(s) 590
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 4 cut(s) 383, 468, 558, 1145
SduI GDGCHC 4 cut(s) 283, 502, 718, 849
SfaNI GCATC 3 cut(s) 811, 890, 1030
SfcI CTRYAG 2 cut(s) 961, 1116
SinI GGWCC 3 cut(s) 308, 560, 1197
SmiMI CAYNNNNRTG 2 cut(s) 303, 1183
SmlI CTYRAG 3 cut(s) 121, 527, 595
SmoI CTYRAG 3 cut(s) 121, 527, 595
SpeI ACTAGT 1 cut(s) 703
Sse9I AATT 9 cut(s) 105, 213, 321, 327, 618, 687, 806, 850, 1002
SsiI CCGC 4 cut(s) 15, 64, 903, 1195
SspI AATATT 2 cut(s) 378, 697
SspMI CTAG 1 cut(s) 704
SstI GAGCTC 1 cut(s) 849
StyD4I CCNGG 4 cut(s) 381, 466, 556, 1143
StyI CCWWGG 3 cut(s) 301, 356, 738
TaaI ACNGT 1 cut(s) 441
TaiI ACGT 1 cut(s) 405
TaqI TCGA 4 cut(s) 137, 765, 833, 898
TasI AATT 9 cut(s) 105, 213, 321, 327, 618, 687, 806, 850, 1002
TatI WGTACW 3 cut(s) 576, 588, 870
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 2 cut(s) 58, 812
Tru1I TTAA 3 cut(s) 80, 104, 1152
Tru9I TTAA 3 cut(s) 80, 104, 1152
TscAI CASTG 2 cut(s) 241, 1129
TseFI GTSAC 3 cut(s) 661, 719, 1120
TseI GCWGC 4 cut(s) 191, 222, 392, 605
Tsp45I GTSAC 3 cut(s) 661, 719, 1120
TspDTI ATGAA 5 cut(s) 61, 798, 876, 919, 1148
TspRI CASTG 2 cut(s) 241, 1129
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 3 cut(s) 308, 560, 1197
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XmiI GTMKAC 1 cut(s) 1092
XspI CTAG 1 cut(s) 704
ZrmI AGTACT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.