MD05G1327200.v1.1

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000662
Physical Location & Seq
Reverse (-)
45323199 .. 45326148
2950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1327200.v1.1.491

Sequence Viewer

Length: 2187 bp
ATGGCTTCCTCCATCAATCCTATTCCTATCTCCTTCAGGAAGCTATGGGAAAGGTGGAATCTACGGGGCTTTATCATCTTAAGCCTAACATTGCAGACCATTTTAATTCTTTGTGCACCCTTCAGAAAGCGAACGCCCAACTTGGTTTTAATCTTCCTAGTCTGGTCATCTTACTTGCTCGCTGACTGGGCTGCTGGCTTTGCAGTCGGGTTAATCTCAAACAGCCAAGGTGACGCTAAAGGTTTGGGAGATAACGAGGAGGATCTCTTGGCATTTTGGGCTCCATTTCTTTTGTTACACCTTGGAGGTCCAGACACCATAACTGCATTTGCTCTTGAAGATAACACTCTGTGGCTAAGGCATTTTCTTGGCCTTGTATTCCAAGTTATAGCTGCAGTGTATGTCTTCATTCAATCGTTTCCCACCAACAAGCTCTGGCCCCCTACACTGCTCCTGTTTCTTGCAGGAACCATTAAGTATGCTGAGCGCACACGTGCTTTGTATCGTGCAAGCTTGGATAATTTCAAGGAATCCATGCTTAAAAAGCCTGATCCTGGACCAAATTATGCAAAGCTAATGGAGGAGTACTCCTCAAAAATTGAATCCAAACTTCCAACTTATATAGAACTCACAGCAGAACGTAGCAAAGAATCCAGGACGGTAACATATGTTGCAGAACCAGGTGATATGGAGAATAATATTGCAGTAGTGCGACATGCTTATCACTTCTATGAAATCTTCAGGGGTCTTATTGTGGATCTCATTTTTAGCTTCCATGAACGCTTCGAGAGCCGTGCATTTTTCCATGAAAGATCCGCAGAAGAAACTTTTAGATTGATTGCAATTGAGCTCAACTTTATGTATGAAGCTCTCTTCACCAAGGCGGTTGTGGTGCATTCTAAGCTAGGATGCCTTTTCCGGGCTATATCCTTCACTGCAGTTTTCATAGCCCTTGTTTTCTTCTATAAACTAGAGAAAAAGGCTTTCCACAAGGTCGATGTTGGAATCACCTATACCCTGCTTTATGGTGCCCTAGGCCTGGATTCAATAGCCATTTTCTTGGTTGTCTTCTCTGAGTGGACTGTCACTGCTATGCACAAGTCTTGGCAAAAATCTTGGGTTGCCACCAAGATACTTGGAAATTACCTCAGTTTGAAGAGACCAAGGTGGTCTACAGAGCCAACTTGCTTGGAGTGGTGTAGACAAATTCTATTTCGAAGGTGGTGCGAATCCATCTCTTCATTCAACTTCATTCACTACTCCCTCAAGGAGCATCGGAAACTGTCCCCTGACATTTTTGACTACCTTGGCATTGGTTACATTGCAATCATCAACTTCTTTGGCCTCAAGGATATTCGAGATAAGATGAAATATCGGACAAGTAGGCCACTCACTAAAGGGTTATGGGAATTCATTTTTCAAGAGTTGAAAGCTAAATCTGTGCTCGCAGATGATCCCGAAACTGCCAAGAGAATATCTATAGCCAGAGGTGATTTCATTCTTCAGGATACCGAGTGGAATAACGCCGAACATGCTACCTTGCTGTCTAGCATTGTTGATGTTGATTATGACCAGAGCATCCTACTGTGGCACATTGCCACTGAATTATGCTATAACACTGAGGAGGAAGAAACTGCTGACTTGGAATCCAGTAAGAATGAAACTTCTCGCCGCGAAATCAGTAAGACCCTGTCGGATTATATGTTGTACCTCTTAGTTATGCAGCCTAGTTTGACGTCTTCTGTAGCAGGAATCGGACAAATAAGATTCCGGGACACATGCGCAGAAGCGAAAAAATTCTTTAGCAGAAGGAAGCTGCCGCTGCGAAAAGGAGAGGAAAACCCAACTCTCTGCACTGTCTGCAAGAGTATCCTCGATGTAAACACAGCTGTTAAACCTGCTGATGTCAAGGGGGATAGAAGTAAATCCGTGTTGTTTGATGCATGTATTTTAGCGAAGAAGTTGAAAGAGATGAAGACAAAGAAATGGGATCTGATGAGTCAAGTATGGGTGGAATTGCTCTCGTATACCGCAGGCCATTGCAGAGCGAACGATCATGTGCAATTGCTGAGTAAAGGAGGAGAACTTGTCACTTTTGTTTGGTTACTCATGGCTCATTTCGGCATAGGGGAGCAGTTCCAGATAAACGAGGGTCATGCTAGAGCAAAACTCATTGTCGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

729

Amino Acids

83.58

Weight (kDa)

8.69

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 420 5.2e-103 Domain of unknown function (DUF4220)
DUF594 PF04578 657 - 709 3.5e-22 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1739
Acc16I TGCGCA 1 cut(s) 1783
Acc36I ACCTGC 1 cut(s) 1906
AccB1I GGYRCC 1 cut(s) 1028
AccI GTMKAC 3 cut(s) 1172, 1201, 2027
AccII CGCG 1 cut(s) 1674
AciI CCGC 5 cut(s) 816, 884, 1672, 1820, 2031
AclWI GGATC 6 cut(s) 270, 545, 765, 807, 1448, 1998
AcsI RAATTY 3 cut(s) 1206, 1409, 1796
AcuI CTGAAG 4 cut(s) 19, 106, 724, 1487
AcvI CACGTG 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 1736
AdeI CACNNNGTG 1 cut(s) 351
AfaI GTAC 2 cut(s) 587, 1709
AfiI CCNNNNNNNGG 3 cut(s) 554, 919, 1039
AflII CTTAAG 1 cut(s) 79
AflIII ACRYGT 1 cut(s) 491
AjnI CCWGG 4 cut(s) 553, 653, 679, 1038
Alw21I GWGCWC 3 cut(s) 118, 852, 1446
Alw26I GTCTC 1 cut(s) 1153
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 6 cut(s) 270, 545, 765, 807, 1448, 1998
AoxI GGCC 6 cut(s) 370, 437, 1036, 1342, 1385, 2035
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 5 cut(s) 191, 392, 1723, 1816, 1822
ApoI RAATTY 3 cut(s) 1206, 1409, 1796
Asp700I GAANNNNTTC 1 cut(s) 1796
AspA2I CCTAGG 1 cut(s) 1033
AspLEI GCGC 2 cut(s) 489, 1784
AspS9I GGNCC 3 cut(s) 308, 438, 557
AsuC2I CCSGG 2 cut(s) 920, 1772
AsuHPI GGTGA 5 cut(s) 242, 695, 868, 1000, 1502
AsuII TTCGAA 1 cut(s) 1216
AvaII GGWCC 2 cut(s) 308, 557
AvrII CCTAGG 1 cut(s) 1033
BaeGI GKGCMC 2 cut(s) 118, 1033
BanI GGYRCC 1 cut(s) 1028
BanII GRGCYC 2 cut(s) 283, 852
BbrPI CACGTG 1 cut(s) 494
BbsI GAAGAC 4 cut(s) 397, 1060, 1731, 1982
Bbv12I GWGCWC 3 cut(s) 118, 852, 1446
BbvI GCAGC 5 cut(s) 178, 379, 1735, 1803, 1809
BccI CCATC 2 cut(s) 20, 1241
BceAI ACGGC 1 cut(s) 777
BcgI CGANNNNNNTGC 6 cut(s) 776, 810, 1206, 1240, 2138, 2172
BciT130I CCWGG 4 cut(s) 555, 655, 681, 1040
BciVI GTATCC 2 cut(s) 1501, 1880
BcnI CCSGG 2 cut(s) 920, 1772
BcoDI GTCTC 1 cut(s) 1153
BfaI CTAG 7 cut(s) 158, 905, 971, 1034, 1548, 1728, 2160
BfmI CTRYAG 5 cut(s) 393, 936, 1173, 1479, 1743
BfrI CTTAAG 1 cut(s) 79
BfuAI ACCTGC 1 cut(s) 1906
BfuI GTATCC 2 cut(s) 1501, 1880
BisI GCNGC 7 cut(s) 192, 393, 1672, 1724, 1817, 1820, 1823
BlnI CCTAGG 1 cut(s) 1033
BlpI GCTNAGC 1 cut(s) 483
BlsI GCNGC 7 cut(s) 193, 394, 1673, 1725, 1818, 1821, 1824
BmcAI AGTACT 1 cut(s) 587
Bme1390I CCNGG 6 cut(s) 555, 655, 681, 920, 1040, 1772
Bme18I GGWCC 2 cut(s) 308, 557
BmgT120I GGNCC 3 cut(s) 308, 438, 557
BmiI GGNNCC 4 cut(s) 282, 440, 469, 1030
BmrFI CCNGG 6 cut(s) 555, 655, 681, 920, 1040, 1772
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 4 cut(s) 899, 1282, 1587, 1930
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 4 cut(s) 397, 1060, 1731, 1982
BplI GAGNNNNNCTC 6 cut(s) 572, 604, 575, 607, 2154, 2186
Bpu10I CCTNAGC 1 cut(s) 356
Bpu1102I GCTNAGC 1 cut(s) 483
Bpu14I TTCGAA 1 cut(s) 1216
BpuEI CTTGAG 2 cut(s) 1250, 1331
BpuMI CCSGG 2 cut(s) 920, 1772
BsaAI YACGTR 1 cut(s) 494
BsaHI GRCGYC 1 cut(s) 1736
BsaI GGTCTC 1 cut(s) 1153
BsaJI CCNNGG 6 cut(s) 226, 301, 879, 1033, 1163, 1306
BsaXI ACNNNNNCTCC 2 cut(s) 2073, 2103
Bsc4I CCNNNNNNNGG 3 cut(s) 554, 919, 1039
Bse1I ACTGG 2 cut(s) 191, 1650
Bse3DI GCAATG 4 cut(s) 89, 1320, 1593, 2038
BseBI CCWGG 4 cut(s) 555, 655, 681, 1040
BseDI CCNNGG 6 cut(s) 226, 301, 879, 1033, 1163, 1306
BseGI GGATG 2 cut(s) 914, 1578
BseLI CCNNNNNNNGG 3 cut(s) 554, 919, 1039
BseMI GCAATG 4 cut(s) 89, 1320, 1593, 2038
BseMII CTCAG 5 cut(s) 474, 1065, 1162, 1611, 2060
BseNI ACTGG 2 cut(s) 191, 1650
BseRI GAGGAG 5 cut(s) 272, 580, 596, 1637, 2094
BseSI GKGCMC 2 cut(s) 118, 1033
BseXI GCAGC 5 cut(s) 178, 379, 1735, 1803, 1809
BsgI GTGCAG 1 cut(s) 1837
Bsh1236I CGCG 1 cut(s) 1674
BshFI GGCC 6 cut(s) 372, 439, 1038, 1344, 1387, 2037
BshNI GGYRCC 1 cut(s) 1028
BsiHKAI GWGCWC 3 cut(s) 118, 852, 1446
BsiSI CCGG 2 cut(s) 919, 1771
BslFI GGGAC 2 cut(s) 1270, 1787
BslI CCNNNNNNNGG 3 cut(s) 554, 919, 1039
BsmAI GTCTC 1 cut(s) 1153
BsmFI GGGAC 2 cut(s) 1270, 1787
BsmI GAATGC 1 cut(s) 895
BsnI GGCC 6 cut(s) 372, 439, 1038, 1344, 1387, 2037
Bso31I GGTCTC 1 cut(s) 1153
Bsp119I TTCGAA 1 cut(s) 1216
Bsp1286I GDGCHC 5 cut(s) 118, 283, 852, 1033, 1446
Bsp143I GATC 7 cut(s) 262, 550, 757, 812, 1453, 1990, 2053
Bsp1720I GCTNAGC 1 cut(s) 483
BspACI CCGC 5 cut(s) 816, 884, 1672, 1820, 2031
BspANI GGCC 6 cut(s) 372, 439, 1038, 1344, 1387, 2037
BspCNI CTCAG 5 cut(s) 475, 1066, 1161, 1612, 2061
BspFNI CGCG 1 cut(s) 1674
BspLI GGNNCC 4 cut(s) 282, 440, 469, 1030
BspMAI CTGCAG 2 cut(s) 397, 940
BspMI ACCTGC 1 cut(s) 1906
BspPI GGATC 6 cut(s) 270, 545, 765, 807, 1448, 1998
BspT104I TTCGAA 1 cut(s) 1216
BspT107I GGYRCC 1 cut(s) 1028
BspTI CTTAAG 1 cut(s) 79
BspTNI GGTCTC 1 cut(s) 1153
BsrDI GCAATG 4 cut(s) 89, 1320, 1593, 2038
BsrI ACTGG 2 cut(s) 191, 1650
BssECI CCNNGG 6 cut(s) 226, 301, 879, 1033, 1163, 1306
BssMI GATC 7 cut(s) 262, 550, 757, 812, 1453, 1990, 2053
BssNAI GTATAC 1 cut(s) 2028
BssNI GRCGYC 1 cut(s) 1736
BssT1I CCWWGG 6 cut(s) 226, 301, 879, 1033, 1163, 1306
Bst1107I GTATAC 1 cut(s) 2028
Bst2UI CCWGG 4 cut(s) 555, 655, 681, 1040
Bst4CI ACNGT 5 cut(s) 661, 1084, 1284, 1587, 1858
Bst6I CTCTTC 3 cut(s) 878, 1151, 1243
BstACI GRCGYC 1 cut(s) 1736
BstAFI CTTAAG 1 cut(s) 79
BstAPI GCANNNNNTGC 1 cut(s) 1860
BstBAI YACGTR 1 cut(s) 494
BstBI TTCGAA 1 cut(s) 1216
BstC8I GCNNGC 5 cut(s) 180, 196, 511, 1446, 2035
BstDEI CTNAG 8 cut(s) 356, 483, 900, 1074, 1148, 1620, 1714, 2069
BstF5I GGATG 2 cut(s) 914, 1578
BstFNI CGCG 1 cut(s) 1674
BstHHI GCGC 2 cut(s) 489, 1784
BstKTI GATC 7 cut(s) 265, 553, 760, 815, 1456, 1993, 2056
BstMAI GTCTC 1 cut(s) 1153
BstMBI GATC 7 cut(s) 262, 550, 757, 812, 1453, 1990, 2053
BstMWI GCNNNNNNNGC 9 cut(s) 188, 200, 278, 544, 789, 901, 1532, 1822, 1860
BstNI CCWGG 4 cut(s) 555, 655, 681, 1040
BstNSI RCATGY 4 cut(s) 719, 1535, 1782, 1947
BstSCI CCNGG 6 cut(s) 553, 653, 679, 918, 1038, 1770
BstSFI CTRYAG 5 cut(s) 393, 936, 1173, 1479, 1743
BstSLI GKGCMC 2 cut(s) 118, 1033
BstUI CGCG 1 cut(s) 1674
BstV1I GCAGC 5 cut(s) 178, 379, 1735, 1803, 1809
BstV2I GAAGAC 4 cut(s) 397, 1060, 1731, 1982
BstX2I RGATCY 4 cut(s) 262, 757, 812, 1990
BstXI CCANNNNNNTGG 1 cut(s) 1060
BstYI RGATCY 4 cut(s) 262, 757, 812, 1990
BstZ17I GTATAC 1 cut(s) 2028
BsuI GTATCC 2 cut(s) 1501, 1880
BsuRI GGCC 6 cut(s) 372, 439, 1038, 1344, 1387, 2037
BtsCI GGATG 2 cut(s) 914, 1578
BtsI GCAGTG 4 cut(s) 402, 446, 933, 1086
BtsIMutI CAGTG 7 cut(s) 402, 446, 933, 1086, 1599, 1617, 1854
BveI ACCTGC 1 cut(s) 1906
Cac8I GCNNGC 5 cut(s) 180, 196, 511, 1446, 2035
CfoI GCGC 2 cut(s) 489, 1784
Cfr13I GGNCC 3 cut(s) 308, 438, 557
CseI GACGC 1 cut(s) 242
CsiI ACCWGGT 1 cut(s) 679
Csp6I GTAC 2 cut(s) 586, 1708
CviQI GTAC 2 cut(s) 586, 1708
DdeI CTNAG 8 cut(s) 356, 483, 900, 1074, 1148, 1620, 1714, 2069
DpnI GATC 7 cut(s) 264, 552, 759, 814, 1455, 1992, 2055
DpnII GATC 7 cut(s) 262, 550, 757, 812, 1453, 1990, 2053
DraIII CACNNNGTG 1 cut(s) 351
Eam1104I CTCTTC 3 cut(s) 878, 1151, 1243
EarI CTCTTC 3 cut(s) 878, 1151, 1243
Ecl136II GAGCTC 1 cut(s) 850
Eco130I CCWWGG 6 cut(s) 226, 301, 879, 1033, 1163, 1306
Eco147I AGGCCT 1 cut(s) 1038
Eco24I GRGCYC 2 cut(s) 283, 852
Eco31I GGTCTC 1 cut(s) 1153
Eco47I GGWCC 2 cut(s) 308, 557
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 4 cut(s) 19, 106, 724, 1487
Eco72I CACGTG 1 cut(s) 494
EcoICRI GAGCTC 1 cut(s) 850
EcoRI GAATTC 1 cut(s) 1409
EcoRII CCWGG 4 cut(s) 553, 653, 679, 1038
EcoT14I CCWWGG 6 cut(s) 226, 301, 879, 1033, 1163, 1306
EcoT22I ATGCAT 1 cut(s) 1945
EcoT38I GRGCYC 2 cut(s) 283, 852
ErhI CCWWGG 6 cut(s) 226, 301, 879, 1033, 1163, 1306
FaqI GGGAC 2 cut(s) 1270, 1787
FauNDI CATATG 1 cut(s) 667
FblI GTMKAC 3 cut(s) 1172, 1201, 2027
Fnu4HI GCNGC 7 cut(s) 192, 393, 1672, 1724, 1817, 1820, 1823
FokI GGATG 2 cut(s) 921, 1565
FriOI GRGCYC 2 cut(s) 283, 852
Fsp4HI GCNGC 7 cut(s) 192, 393, 1672, 1724, 1817, 1820, 1823
FspBI CTAG 7 cut(s) 158, 905, 971, 1034, 1548, 1728, 2160
FspI TGCGCA 1 cut(s) 1783
GlaI GCGC 2 cut(s) 488, 1783
GluI GCNGC 7 cut(s) 192, 393, 1672, 1724, 1817, 1820, 1823
HaeIII GGCC 6 cut(s) 372, 439, 1038, 1344, 1387, 2037
HapII CCGG 2 cut(s) 919, 1771
HgaI GACGC 1 cut(s) 242
HhaI GCGC 2 cut(s) 489, 1784
Hin1I GRCGYC 1 cut(s) 1736
Hin6I GCGC 2 cut(s) 487, 1782
HinP1I GCGC 2 cut(s) 487, 1782
HindIII AAGCTT 1 cut(s) 511
HpaII CCGG 2 cut(s) 919, 1771
HphI GGTGA 5 cut(s) 242, 695, 868, 1000, 1502
Hpy166II GTNNAC 6 cut(s) 116, 1080, 1173, 1202, 1882, 2028
Hpy188I TCNGA 8 cut(s) 125, 1075, 1278, 1377, 1696, 1757, 1995, 2180
Hpy188III TCNNGA 9 cut(s) 37, 311, 335, 787, 1358, 1421, 1457, 1505, 2140
Hpy8I GTNNAC 6 cut(s) 116, 1080, 1173, 1202, 1882, 2028
HpyAV CCTTC 5 cut(s) 43, 130, 940, 1212, 1803
HpyCH4III ACNGT 5 cut(s) 661, 1084, 1284, 1587, 1858
HpyCH4IV ACGT 3 cut(s) 493, 640, 1736
HpyF10VI GCNNNNNNNGC 9 cut(s) 188, 200, 278, 544, 789, 901, 1532, 1822, 1860
HpyF3I CTNAG 8 cut(s) 356, 483, 900, 1074, 1148, 1620, 1714, 2069
HpySE526I ACGT 3 cut(s) 493, 640, 1736
Hsp92I GRCGYC 1 cut(s) 1736
HspAI GCGC 2 cut(s) 487, 1782
Kzo9I GATC 7 cut(s) 262, 550, 757, 812, 1453, 1990, 2053
LmnI GCTCC 4 cut(s) 286, 456, 1270, 2131
Lsp1109I GCAGC 5 cut(s) 178, 379, 1735, 1803, 1809
LweI GCATC 4 cut(s) 899, 1282, 1587, 1930
MabI ACCWGGT 1 cut(s) 679
MaeI CTAG 7 cut(s) 158, 905, 971, 1034, 1548, 1728, 2160
MaeII ACGT 3 cut(s) 493, 640, 1736
MaeIII GTNAC 7 cut(s) 230, 294, 661, 1084, 1316, 2089, 2103
MalI GATC 7 cut(s) 264, 552, 759, 814, 1455, 1992, 2055
MboI GATC 7 cut(s) 262, 550, 757, 812, 1453, 1990, 2053
MfeI CAATTG 2 cut(s) 843, 2063
MflI RGATCY 4 cut(s) 262, 757, 812, 1990
MhlI GDGCHC 5 cut(s) 118, 283, 852, 1033, 1446
MlyI GAGTC 1 cut(s) 2008
MmeI TCCRAC 4 cut(s) 638, 982, 1674, 2158
Mph1103I ATGCAT 1 cut(s) 1945
MroXI GAANNNNTTC 1 cut(s) 1796
MseI TTAA 7 cut(s) 80, 104, 149, 212, 474, 540, 1893
MslI CAYNNNNRTG 2 cut(s) 729, 1091
MspA1I CMGCKG 2 cut(s) 1822, 1889
MspCI CTTAAG 1 cut(s) 79
MspI CCGG 2 cut(s) 919, 1771
MspR9I CCNGG 6 cut(s) 555, 655, 681, 920, 1040, 1772
MunI CAATTG 2 cut(s) 843, 2063
Mva1269I GAATGC 1 cut(s) 895
MvaI CCWGG 4 cut(s) 555, 655, 681, 1040
MvnI CGCG 1 cut(s) 1674
MwoI GCNNNNNNNGC 9 cut(s) 188, 200, 278, 544, 789, 901, 1532, 1822, 1860
NciI CCSGG 2 cut(s) 920, 1772
NdeI CATATG 1 cut(s) 667
NdeII GATC 7 cut(s) 262, 550, 757, 812, 1453, 1990, 2053
NlaIV GGNNCC 4 cut(s) 282, 440, 469, 1030
NmuCI GTSAC 3 cut(s) 230, 1084, 2089
NsbI TGCGCA 1 cut(s) 1783
NsiI ATGCAT 1 cut(s) 1945
NspI RCATGY 4 cut(s) 719, 1535, 1782, 1947
NspV TTCGAA 1 cut(s) 1216
PceI AGGCCT 1 cut(s) 1038
PctI GAATGC 1 cut(s) 895
PdmI GAANNNNTTC 1 cut(s) 1796
PflFI GACNNNGTC 1 cut(s) 1690
PfoI TCCNGGA 3 cut(s) 553, 653, 1770
PkrI GCNGC 7 cut(s) 193, 394, 1673, 1725, 1818, 1821, 1824
PleI GAGTC 1 cut(s) 2007
PmaCI CACGTG 1 cut(s) 494
PmlI CACGTG 1 cut(s) 494
PpsI GAGTC 1 cut(s) 2007
Ppu21I YACGTR 1 cut(s) 494
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 4 cut(s) 553, 653, 679, 1038
PspCI CACGTG 1 cut(s) 494
PspGI CCWGG 4 cut(s) 553, 653, 679, 1038
PspN4I GGNNCC 4 cut(s) 282, 440, 469, 1030
PspPI GGNCC 3 cut(s) 308, 438, 557
PstI CTGCAG 2 cut(s) 397, 940
PsuI RGATCY 4 cut(s) 262, 757, 812, 1990
PsyI GACNNNGTC 1 cut(s) 1690
PvuII CAGCTG 1 cut(s) 1889
RsaI GTAC 2 cut(s) 587, 1709
RsaNI GTAC 2 cut(s) 586, 1708
RseI CAYNNNNRTG 2 cut(s) 729, 1091
SacI GAGCTC 1 cut(s) 852
SaqAI TTAA 7 cut(s) 80, 104, 149, 212, 474, 540, 1893
SatI GCNGC 7 cut(s) 192, 393, 1672, 1724, 1817, 1820, 1823
Sau3AI GATC 7 cut(s) 262, 550, 757, 812, 1453, 1990, 2053
Sau96I GGNCC 3 cut(s) 308, 438, 557
ScaI AGTACT 1 cut(s) 587
SchI GAGTC 1 cut(s) 2008
ScrFI CCNGG 6 cut(s) 555, 655, 681, 920, 1040, 1772
SduI GDGCHC 5 cut(s) 118, 283, 852, 1033, 1446
SexAI ACCWGGT 1 cut(s) 679
SfaNI GCATC 4 cut(s) 899, 1282, 1587, 1930
SfcI CTRYAG 5 cut(s) 393, 936, 1173, 1479, 1743
SfuI TTCGAA 1 cut(s) 1216
SinI GGWCC 2 cut(s) 308, 557
SmiMI CAYNNNNRTG 2 cut(s) 729, 1091
SmlI CTYRAG 3 cut(s) 79, 1265, 1346
SmoI CTYRAG 3 cut(s) 79, 1265, 1346
SseBI AGGCCT 1 cut(s) 1038
SsiI CCGC 5 cut(s) 816, 884, 1672, 1820, 2031
SspI AATATT 1 cut(s) 700
SspMI CTAG 7 cut(s) 158, 905, 971, 1034, 1548, 1728, 2160
SstI GAGCTC 1 cut(s) 852
StuI AGGCCT 1 cut(s) 1038
StyD4I CCNGG 6 cut(s) 553, 653, 679, 918, 1038, 1770
StyI CCWWGG 6 cut(s) 226, 301, 879, 1033, 1163, 1306
TaaI ACNGT 5 cut(s) 661, 1084, 1284, 1587, 1858
TaiI ACGT 3 cut(s) 496, 643, 1739
TaqI TCGA 5 cut(s) 786, 996, 1216, 1357, 1875
TatI WGTACW 1 cut(s) 585
TauI GCSGC 2 cut(s) 1674, 1822
Tru1I TTAA 7 cut(s) 80, 104, 149, 212, 474, 540, 1893
Tru9I TTAA 7 cut(s) 80, 104, 149, 212, 474, 540, 1893
TscAI CASTG 7 cut(s) 402, 453, 940, 1093, 1606, 1624, 1861
TseFI GTSAC 3 cut(s) 230, 1084, 2089
TseI GCWGC 5 cut(s) 191, 392, 1723, 1816, 1822
Tsp45I GTSAC 3 cut(s) 230, 1084, 2089
TspGWI ACGGA 1 cut(s) 1918
TspRI CASTG 7 cut(s) 402, 453, 940, 1093, 1606, 1624, 1861
Tth111I GACNNNGTC 1 cut(s) 1690
Vha464I CTTAAG 1 cut(s) 79
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 2 cut(s) 308, 557
XapI RAATTY 3 cut(s) 1206, 1409, 1796
XceI RCATGY 4 cut(s) 719, 1535, 1782, 1947
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XmaJI CCTAGG 1 cut(s) 1033
XmiI GTMKAC 3 cut(s) 1172, 1201, 2027
XmnI GAANNNNTTC 1 cut(s) 1796
XspI CTAG 7 cut(s) 158, 905, 971, 1034, 1548, 1728, 2160
ZraI GACGTC 1 cut(s) 1737
ZrmI AGTACT 1 cut(s) 587
Zsp2I ATGCAT 1 cut(s) 1945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.