Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 362 |
| Acc36I | ACCTGC | 1 cut(s) | 362 |
| AccB1I | GGYRCC | 2 cut(s) | 81, 259 |
| AccI | GTMKAC | 2 cut(s) | 74, 797 |
| AccII | CGCG | 3 cut(s) | 485, 612, 702 |
| AciI | CCGC | 3 cut(s) | 612, 1397, 1792 |
| AclWI | GGATC | 6 cut(s) | 218, 852, 865, 898, 1234, 1247 |
| AcoI | YGGCCR | 1 cut(s) | 2061 |
| AcsI | RAATTY | 3 cut(s) | 911, 1558, 1977 |
| AcuI | CTGAAG | 4 cut(s) | 650, 706, 1115, 2057 |
| AcyI | GRCGYC | 2 cut(s) | 5, 521 |
| AdeI | CACNNNGTG | 3 cut(s) | 88, 1384, 2162 |
| AfaI | GTAC | 5 cut(s) | 45, 408, 479, 500, 1470 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 531, 1050 |
| AflIII | ACRYGT | 3 cut(s) | 987, 1121, 2174 |
| AgsI | TTSAA | 6 cut(s) | 531, 677, 1441, 1465, 1643, 1924 |
| AjnI | CCWGG | 4 cut(s) | 77, 1250, 1399, 1578 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2076, 2108 |
| AleI | CACNNNNGTG | 1 cut(s) | 282 |
| Alw21I | GWGCWC | 2 cut(s) | 774, 1360 |
| Alw26I | GTCTC | 3 cut(s) | 399, 1887, 1938 |
| AlwI | GGATC | 6 cut(s) | 218, 852, 865, 898, 1234, 1247 |
| AoxI | GGCC | 4 cut(s) | 714, 1100, 2061, 2242 |
| ApeKI | GCWGC | 5 cut(s) | 697, 1549, 1663, 1843, 2150 |
| ApoI | RAATTY | 3 cut(s) | 911, 1558, 1977 |
| AspA2I | CCTAGG | 3 cut(s) | 1513, 2006, 2245 |
| AspLEI | GCGC | 2 cut(s) | 367, 702 |
| AspS9I | GGNCC | 2 cut(s) | 136, 287 |
| AsuHPI | GGTGA | 4 cut(s) | 427, 868, 928, 1486 |
| AvaII | GGWCC | 2 cut(s) | 136, 287 |
| AvrII | CCTAGG | 3 cut(s) | 1513, 2006, 2245 |
| BaeGI | GKGCMC | 1 cut(s) | 262 |
| BalI | TGGCCA | 1 cut(s) | 2063 |
| BamHI | GGATCC | 2 cut(s) | 857, 1239 |
| BanI | GGYRCC | 2 cut(s) | 81, 259 |
| BanII | GRGCYC | 1 cut(s) | 774 |
| BauI | CACGAG | 1 cut(s) | 107 |
| BbsI | GAAGAC | 2 cut(s) | 324, 2106 |
| Bbv12I | GWGCWC | 2 cut(s) | 774, 1360 |
| BbvI | GCAGC | 5 cut(s) | 684, 1536, 1650, 1855, 2162 |
| BccI | CCATC | 8 cut(s) | 61, 305, 887, 989, 1025, 1170, 1322, 1924 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 305, 339, 1106, 1140 |
| BciT130I | CCWGG | 4 cut(s) | 79, 1252, 1401, 1580 |
| BciVI | GTATCC | 1 cut(s) | 1077 |
| BclI | TGATCA | 2 cut(s) | 933, 1836 |
| BcoDI | GTCTC | 3 cut(s) | 399, 1887, 1938 |
| BfaI | CTAG | 6 cut(s) | 1514, 1608, 1626, 1950, 2007, 2246 |
| BfoI | RGCGCY | 1 cut(s) | 368 |
| BfuAI | ACCTGC | 1 cut(s) | 362 |
| BfuI | GTATCC | 1 cut(s) | 1077 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 257 |
| BisI | GCNGC | 5 cut(s) | 698, 1550, 1664, 1844, 2151 |
| BlnI | CCTAGG | 3 cut(s) | 1513, 2006, 2245 |
| BlpI | GCTNAGC | 1 cut(s) | 1026 |
| BlsI | GCNGC | 5 cut(s) | 699, 1551, 1665, 1845, 2152 |
| Bme1390I | CCNGG | 4 cut(s) | 79, 1252, 1401, 1580 |
| Bme18I | GGWCC | 2 cut(s) | 136, 287 |
| BmgT120I | GGNCC | 2 cut(s) | 136, 287 |
| BmiI | GGNNCC | 6 cut(s) | 83, 138, 153, 261, 859, 1241 |
| BmrFI | CCNGG | 4 cut(s) | 79, 1252, 1401, 1580 |
| BmrI | ACTGGG | 1 cut(s) | 863 |
| BmsI | GCATC | 7 cut(s) | 616, 1197, 1443, 1527, 1631, 2155, 2162 |
| BmuI | ACTGGG | 1 cut(s) | 863 |
| BoxI | GACNNNNGTC | 1 cut(s) | 835 |
| BpiI | GAAGAC | 2 cut(s) | 324, 2106 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2039, 2071 |
| BpmI | CTGGAG | 1 cut(s) | 1601 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1848 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1026 |
| BpuEI | CTTGAG | 1 cut(s) | 686 |
| BsaAI | YACGTR | 2 cut(s) | 43, 2177 |
| BsaBI | GATNNNNATC | 2 cut(s) | 847, 1934 |
| BsaHI | GRCGYC | 2 cut(s) | 5, 521 |
| BsaJI | CCNNGG | 8 cut(s) | 199, 280, 1250, 1400, 1513, 1905, 2006, 2245 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 531, 1050 |
| Bse1I | ACTGG | 8 cut(s) | 128, 391, 858, 1010, 1085, 1186, 1370, 2064 |
| Bse3DI | GCAATG | 2 cut(s) | 435, 728 |
| Bse8I | GATNNNNATC | 2 cut(s) | 847, 1934 |
| BseBI | CCWGG | 4 cut(s) | 79, 1252, 1401, 1580 |
| BseDI | CCNNGG | 8 cut(s) | 199, 280, 1250, 1400, 1513, 1905, 2006, 2245 |
| BseGI | GGATG | 6 cut(s) | 976, 981, 1017, 1042, 1317, 1374 |
| BseJI | GATNNNNATC | 2 cut(s) | 847, 1934 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 531, 1050 |
| BseMI | GCAATG | 2 cut(s) | 435, 728 |
| BseMII | CTCAG | 5 cut(s) | 61, 138, 579, 1183, 1767 |
| BseNI | ACTGG | 8 cut(s) | 128, 391, 858, 1010, 1085, 1186, 1370, 2064 |
| BseRI | GAGGAG | 2 cut(s) | 164, 219 |
| BseSI | GKGCMC | 1 cut(s) | 262 |
| BseXI | GCAGC | 5 cut(s) | 684, 1536, 1650, 1855, 2162 |
| BseYI | CCCAGC | 1 cut(s) | 440 |
| Bsh1236I | CGCG | 3 cut(s) | 485, 612, 702 |
| BshFI | GGCC | 4 cut(s) | 716, 1102, 2063, 2244 |
| BshNI | GGYRCC | 2 cut(s) | 81, 259 |
| BsiHKAI | GWGCWC | 2 cut(s) | 774, 1360 |
| BslFI | GGGAC | 3 cut(s) | 122, 457, 572 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 531, 1050 |
| BsmAI | GTCTC | 3 cut(s) | 399, 1887, 1938 |
| BsmFI | GGGAC | 3 cut(s) | 122, 457, 572 |
| BsmI | GAATGC | 1 cut(s) | 683 |
| BsnI | GGCC | 4 cut(s) | 716, 1102, 2063, 2244 |
| Bsp1286I | GDGCHC | 3 cut(s) | 262, 774, 1360 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1026 |
| Bsp68I | TCGCGA | 1 cut(s) | 485 |
| BspACI | CCGC | 3 cut(s) | 612, 1397, 1792 |
| BspANI | GGCC | 4 cut(s) | 716, 1102, 2063, 2244 |
| BspCNI | CTCAG | 5 cut(s) | 60, 139, 580, 1182, 1768 |
| BspFNI | CGCG | 3 cut(s) | 485, 612, 702 |
| BspLI | GGNNCC | 6 cut(s) | 83, 138, 153, 261, 859, 1241 |
| BspMI | ACCTGC | 1 cut(s) | 362 |
| BspPI | GGATC | 6 cut(s) | 218, 852, 865, 898, 1234, 1247 |
| BspQI | GCTCTTC | 1 cut(s) | 1365 |
| BspT107I | GGYRCC | 2 cut(s) | 81, 259 |
| BsrDI | GCAATG | 2 cut(s) | 435, 728 |
| BsrI | ACTGG | 8 cut(s) | 128, 391, 858, 1010, 1085, 1186, 1370, 2064 |
| BssECI | CCNNGG | 8 cut(s) | 199, 280, 1250, 1400, 1513, 1905, 2006, 2245 |
| BssNAI | GTATAC | 1 cut(s) | 798 |
| BssNI | GRCGYC | 2 cut(s) | 5, 521 |
| BssSI | CACGAG | 1 cut(s) | 107 |
| BssT1I | CCWWGG | 5 cut(s) | 199, 280, 1513, 2006, 2245 |
| Bst1107I | GTATAC | 1 cut(s) | 798 |
| Bst2BI | CACGAG | 1 cut(s) | 107 |
| Bst2UI | CCWGG | 4 cut(s) | 79, 1252, 1401, 1580 |
| Bst4CI | ACNGT | 3 cut(s) | 795, 1161, 1700 |
| Bst6I | CTCTTC | 3 cut(s) | 1091, 1365, 2147 |
| BstACI | GRCGYC | 2 cut(s) | 5, 521 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 188, 1147 |
| BstBAI | YACGTR | 2 cut(s) | 43, 2177 |
| BstC8I | GCNNGC | 7 cut(s) | 369, 519, 1264, 1268, 1870, 1969, 2053 |
| BstF5I | GGATG | 6 cut(s) | 976, 981, 1017, 1042, 1317, 1374 |
| BstFNI | CGCG | 3 cut(s) | 485, 612, 702 |
| BstH2I | RGCGCY | 1 cut(s) | 368 |
| BstHHI | GCGC | 2 cut(s) | 367, 702 |
| BstMAI | GTCTC | 3 cut(s) | 399, 1887, 1938 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 188, 257, 708, 1022, 1147, 1800, 1849 |
| BstNI | CCWGG | 4 cut(s) | 79, 1252, 1401, 1580 |
| BstNSI | RCATGY | 4 cut(s) | 119, 991, 1125, 1266 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 835 |
| BstSCI | CCNGG | 4 cut(s) | 77, 1250, 1399, 1578 |
| BstSLI | GKGCMC | 1 cut(s) | 262 |
| BstSNI | TACGTA | 1 cut(s) | 43 |
| BstUI | CGCG | 3 cut(s) | 485, 612, 702 |
| BstV1I | GCAGC | 5 cut(s) | 684, 1536, 1650, 1855, 2162 |
| BstV2I | GAAGAC | 2 cut(s) | 324, 2106 |
| BstX2I | RGATCY | 3 cut(s) | 857, 903, 1239 |
| BstYI | RGATCY | 3 cut(s) | 857, 903, 1239 |
| BstZ17I | GTATAC | 1 cut(s) | 798 |
| BsuI | GTATCC | 1 cut(s) | 1077 |
| BsuRI | GGCC | 4 cut(s) | 716, 1102, 2063, 2244 |
| BtgZI | GCGATG | 3 cut(s) | 593, 717, 1702 |
| BtsCI | GGATG | 6 cut(s) | 976, 981, 1017, 1042, 1317, 1374 |
| BtsI | GCAGTG | 1 cut(s) | 300 |
| BtsIMutI | CAGTG | 6 cut(s) | 300, 730, 851, 1363, 2043, 2057 |
| BtuMI | TCGCGA | 1 cut(s) | 485 |
| BveI | ACCTGC | 1 cut(s) | 362 |
| Cac8I | GCNNGC | 7 cut(s) | 369, 519, 1264, 1268, 1870, 1969, 2053 |
| CfoI | GCGC | 2 cut(s) | 367, 702 |
| Cfr13I | GGNCC | 2 cut(s) | 136, 287 |
| CseI | GACGC | 1 cut(s) | 510 |
| Csp6I | GTAC | 5 cut(s) | 44, 407, 478, 499, 1469 |
| CviQI | GTAC | 5 cut(s) | 44, 407, 478, 499, 1469 |
| DraIII | CACNNNGTG | 3 cut(s) | 88, 1384, 2162 |
| EaeI | YGGCCR | 1 cut(s) | 2061 |
| Eam1104I | CTCTTC | 3 cut(s) | 1091, 1365, 2147 |
| EarI | CTCTTC | 3 cut(s) | 1091, 1365, 2147 |
| Ecl136II | GAGCTC | 1 cut(s) | 772 |
| Eco105I | TACGTA | 1 cut(s) | 43 |
| Eco130I | CCWWGG | 5 cut(s) | 199, 280, 1513, 2006, 2245 |
| Eco147I | AGGCCT | 1 cut(s) | 716 |
| Eco24I | GRGCYC | 1 cut(s) | 774 |
| Eco47I | GGWCC | 2 cut(s) | 136, 287 |
| Eco53kI | GAGCTC | 1 cut(s) | 772 |
| Eco57I | CTGAAG | 4 cut(s) | 650, 706, 1115, 2057 |
| EcoICRI | GAGCTC | 1 cut(s) | 772 |
| EcoO109I | RGGNCCY | 1 cut(s) | 136 |
| EcoRII | CCWGG | 4 cut(s) | 77, 1250, 1399, 1578 |
| EcoT14I | CCWWGG | 5 cut(s) | 199, 280, 1513, 2006, 2245 |
| EcoT22I | ATGCAT | 3 cut(s) | 38, 121, 685 |
| EcoT38I | GRGCYC | 1 cut(s) | 774 |
| ErhI | CCWWGG | 5 cut(s) | 199, 280, 1513, 2006, 2245 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 704, 736 |
| FaqI | GGGAC | 3 cut(s) | 122, 457, 572 |
| FbaI | TGATCA | 2 cut(s) | 933, 1836 |
| FblI | GTMKAC | 2 cut(s) | 74, 797 |
| Fnu4HI | GCNGC | 5 cut(s) | 698, 1550, 1664, 1844, 2151 |
| FokI | GGATG | 6 cut(s) | 968, 983, 1004, 1049, 1304, 1361 |
| FriOI | GRGCYC | 1 cut(s) | 774 |
| Fsp4HI | GCNGC | 5 cut(s) | 698, 1550, 1664, 1844, 2151 |
| FspBI | CTAG | 6 cut(s) | 1514, 1608, 1626, 1950, 2007, 2246 |
| GlaI | GCGC | 2 cut(s) | 366, 701 |
| GluI | GCNGC | 5 cut(s) | 698, 1550, 1664, 1844, 2151 |
| GsaI | CCCAGC | 1 cut(s) | 444 |
| GsuI | CTGGAG | 1 cut(s) | 1601 |
| HaeII | RGCGCY | 1 cut(s) | 368 |
| HaeIII | GGCC | 4 cut(s) | 716, 1102, 2063, 2244 |
| HgaI | GACGC | 1 cut(s) | 510 |
| HhaI | GCGC | 2 cut(s) | 367, 702 |
| Hin1I | GRCGYC | 2 cut(s) | 5, 521 |
| Hin6I | GCGC | 2 cut(s) | 365, 700 |
| HinP1I | GCGC | 2 cut(s) | 365, 700 |
| HincII | GTYRAC | 2 cut(s) | 9, 657 |
| HindII | GTYRAC | 2 cut(s) | 9, 657 |
| HindIII | AAGCTT | 1 cut(s) | 747 |
| HinfI | GANTC | 8 cut(s) | 71, 670, 843, 1567, 1639, 1748, 1887, 1946 |
| HpaI | GTTAAC | 1 cut(s) | 657 |
| HphI | GGTGA | 4 cut(s) | 427, 868, 928, 1486 |
| Hpy166II | GTNNAC | 5 cut(s) | 9, 75, 287, 657, 798 |
| Hpy8I | GTNNAC | 5 cut(s) | 9, 75, 287, 657, 798 |
| Hpy99I | CGWCG | 2 cut(s) | 251, 1049 |
| HpyAV | CCTTC | 6 cut(s) | 595, 727, 782, 872, 883, 1216 |
| HpyCH4III | ACNGT | 3 cut(s) | 795, 1161, 1700 |
| HpyCH4IV | ACGT | 3 cut(s) | 42, 944, 2176 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 188, 257, 708, 1022, 1147, 1800, 1849 |
| HpySE526I | ACGT | 3 cut(s) | 42, 944, 2176 |
| Hsp92I | GRCGYC | 2 cut(s) | 5, 521 |
| HspAI | GCGC | 2 cut(s) | 365, 700 |
| Ksp22I | TGATCA | 2 cut(s) | 933, 1836 |
| KspAI | GTTAAC | 1 cut(s) | 657 |
| LguI | GCTCTTC | 1 cut(s) | 1365 |
| LmnI | GCTCC | 4 cut(s) | 151, 604, 694, 2182 |
| Lsp1109I | GCAGC | 5 cut(s) | 684, 1536, 1650, 1855, 2162 |
| LweI | GCATC | 7 cut(s) | 616, 1197, 1443, 1527, 1631, 2155, 2162 |
| MaeI | CTAG | 6 cut(s) | 1514, 1608, 1626, 1950, 2007, 2246 |
| MaeII | ACGT | 3 cut(s) | 42, 944, 2176 |
| MaeIII | GTNAC | 6 cut(s) | 88, 109, 837, 1334, 1492, 1733 |
| MfeI | CAATTG | 2 cut(s) | 156, 468 |
| MflI | RGATCY | 3 cut(s) | 857, 903, 1239 |
| MhlI | GDGCHC | 3 cut(s) | 262, 774, 1360 |
| MlsI | TGGCCA | 1 cut(s) | 2063 |
| MluCI | AATT | 7 cut(s) | 156, 468, 526, 911, 1558, 1961, 1977 |
| MluNI | TGGCCA | 1 cut(s) | 2063 |
| MlyI | GAGTC | 2 cut(s) | 80, 1940 |
| MmeI | TCCRAC | 2 cut(s) | 808, 2112 |
| Mox20I | TGGCCA | 1 cut(s) | 2063 |
| Mph1103I | ATGCAT | 3 cut(s) | 38, 121, 685 |
| MscI | TGGCCA | 1 cut(s) | 2063 |
| MseI | TTAA | 4 cut(s) | 213, 656, 1152, 1199 |
| MslI | CAYNNNNRTG | 5 cut(s) | 282, 986, 992, 1193, 2163 |
| Msp20I | TGGCCA | 1 cut(s) | 2063 |
| MspR9I | CCNGG | 4 cut(s) | 79, 1252, 1401, 1580 |
| MunI | CAATTG | 2 cut(s) | 156, 468 |
| Mva1269I | GAATGC | 1 cut(s) | 683 |
| MvaI | CCWGG | 4 cut(s) | 79, 1252, 1401, 1580 |
| MvnI | CGCG | 3 cut(s) | 485, 612, 702 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 188, 257, 708, 1022, 1147, 1800, 1849 |
| NlaIV | GGNNCC | 6 cut(s) | 83, 138, 153, 261, 859, 1241 |
| NmeAIII | GCCGAG | 2 cut(s) | 793, 1930 |
| NmuCI | GTSAC | 4 cut(s) | 88, 109, 837, 1492 |
| NruI | TCGCGA | 1 cut(s) | 485 |
| NsiI | ATGCAT | 3 cut(s) | 38, 121, 685 |
| NspI | RCATGY | 4 cut(s) | 119, 991, 1125, 1266 |
| OliI | CACNNNNGTG | 1 cut(s) | 282 |
| PaeI | GCATGC | 1 cut(s) | 1266 |
| PaqCI | CACCTGC | 1 cut(s) | 362 |
| PceI | AGGCCT | 1 cut(s) | 716 |
| PciI | ACATGT | 2 cut(s) | 987, 1121 |
| PciSI | GCTCTTC | 1 cut(s) | 1365 |
| PctI | GAATGC | 1 cut(s) | 683 |
| PfeI | GAWTC | 6 cut(s) | 670, 843, 1567, 1639, 1748, 1887 |
| PkrI | GCNGC | 5 cut(s) | 699, 1551, 1665, 1845, 2152 |
| PleI | GAGTC | 2 cut(s) | 79, 1940 |
| PpsI | GAGTC | 2 cut(s) | 79, 1940 |
| Ppu21I | YACGTR | 2 cut(s) | 43, 2177 |
| PpuMI | RGGWCCY | 1 cut(s) | 136 |
| PscI | ACATGT | 2 cut(s) | 987, 1121 |
| PshAI | GACNNNNGTC | 1 cut(s) | 835 |
| Psp124BI | GAGCTC | 1 cut(s) | 774 |
| Psp5II | RGGWCCY | 1 cut(s) | 136 |
| Psp6I | CCWGG | 4 cut(s) | 77, 1250, 1399, 1578 |
| PspFI | CCCAGC | 1 cut(s) | 440 |
| PspGI | CCWGG | 4 cut(s) | 77, 1250, 1399, 1578 |
| PspN4I | GGNNCC | 6 cut(s) | 83, 138, 153, 261, 859, 1241 |
| PspPI | GGNCC | 2 cut(s) | 136, 287 |
| PspPPI | RGGWCCY | 1 cut(s) | 136 |
| PsuI | RGATCY | 3 cut(s) | 857, 903, 1239 |
| RruI | TCGCGA | 1 cut(s) | 485 |
| RsaI | GTAC | 5 cut(s) | 45, 408, 479, 500, 1470 |
| RsaNI | GTAC | 5 cut(s) | 44, 407, 478, 499, 1469 |
| RseI | CAYNNNNRTG | 5 cut(s) | 282, 986, 992, 1193, 2163 |
| SacI | GAGCTC | 1 cut(s) | 774 |
| SapI | GCTCTTC | 1 cut(s) | 1365 |
| SaqAI | TTAA | 4 cut(s) | 213, 656, 1152, 1199 |
| SatI | GCNGC | 5 cut(s) | 698, 1550, 1664, 1844, 2151 |
| Sau96I | GGNCC | 2 cut(s) | 136, 287 |
| SchI | GAGTC | 2 cut(s) | 80, 1940 |
| ScrFI | CCNGG | 4 cut(s) | 79, 1252, 1401, 1580 |
| SduI | GDGCHC | 3 cut(s) | 262, 774, 1360 |
| SfaNI | GCATC | 7 cut(s) | 616, 1197, 1443, 1527, 1631, 2155, 2162 |
| SinI | GGWCC | 2 cut(s) | 136, 287 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 282, 986, 992, 1193, 2163 |
| SmlI | CTYRAG | 1 cut(s) | 665 |
| SmoI | CTYRAG | 1 cut(s) | 665 |
| SnaBI | TACGTA | 1 cut(s) | 43 |
| SphI | GCATGC | 1 cut(s) | 1266 |
| Sse9I | AATT | 7 cut(s) | 156, 468, 526, 911, 1558, 1961, 1977 |
| SseBI | AGGCCT | 1 cut(s) | 716 |
| SsiI | CCGC | 3 cut(s) | 612, 1397, 1792 |
| SspMI | CTAG | 6 cut(s) | 1514, 1608, 1626, 1950, 2007, 2246 |
| SstI | GAGCTC | 1 cut(s) | 774 |
| StuI | AGGCCT | 1 cut(s) | 716 |
| StyD4I | CCNGG | 4 cut(s) | 77, 1250, 1399, 1578 |
| StyI | CCWWGG | 5 cut(s) | 199, 280, 1513, 2006, 2245 |
| TaaI | ACNGT | 3 cut(s) | 795, 1161, 1700 |
| TaiI | ACGT | 3 cut(s) | 45, 947, 2179 |
| TaqI | TCGA | 4 cut(s) | 315, 509, 774, 2001 |
| TasI | AATT | 7 cut(s) | 156, 468, 526, 911, 1558, 1961, 1977 |
| TatI | WGTACW | 2 cut(s) | 406, 498 |
| TfiI | GAWTC | 6 cut(s) | 670, 843, 1567, 1639, 1748, 1887 |
| Tru1I | TTAA | 4 cut(s) | 213, 656, 1152, 1199 |
| Tru9I | TTAA | 4 cut(s) | 213, 656, 1152, 1199 |
| TscAI | CASTG | 6 cut(s) | 307, 730, 858, 1370, 2043, 2064 |
| TseFI | GTSAC | 4 cut(s) | 88, 109, 837, 1492 |
| TseI | GCWGC | 5 cut(s) | 697, 1549, 1663, 1843, 2150 |
| Tsp45I | GTSAC | 4 cut(s) | 88, 109, 837, 1492 |
| TspGWI | ACGGA | 3 cut(s) | 26, 478, 1489 |
| TspRI | CASTG | 6 cut(s) | 307, 730, 858, 1370, 2043, 2064 |
| VpaK11BI | GGWCC | 2 cut(s) | 136, 287 |
| XapI | RAATTY | 3 cut(s) | 911, 1558, 1977 |
| XbaI | TCTAGA | 1 cut(s) | 1949 |
| XceI | RCATGY | 4 cut(s) | 119, 991, 1125, 1266 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1110 |
| XmaJI | CCTAGG | 3 cut(s) | 1513, 2006, 2245 |
| XmiI | GTMKAC | 2 cut(s) | 74, 797 |
| XspI | CTAG | 6 cut(s) | 1514, 1608, 1626, 1950, 2007, 2246 |
| Zsp2I | ATGCAT | 3 cut(s) | 38, 121, 685 |