pycom05g30160

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000662
Physical Location & Seq
Reverse (-)
30362995 .. 30365221
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30160.1

Sequence Viewer

Length: 1920 bp
ATGGGAAGGGTGGAATCTACGGGGCTTTATCATCTTAAGCCTAACATTGCAGACCATGTTAATTCTTTGTGCACCCTTCAGAAAGTGAACACCCAACTTGTTTTTAATCTTCCTGGAACCATTAAGTATGCTGAGCGCACACGTGCCTTGTATCGTGCAAGCTTGGATAATTTCAAGGAATCCTTGATTAAAAAGCCTGATCCTGGACCAAATTATGCAAAGCTAATGGAGGAGTACTCTTCAAAAAAAGATGCCAAACTTCCAACTCGTATAGAACTCACAGCAGAACGAAGCAAAGAATCCAGGAAAGTAACATATGTTGCAGAACAAGGTGATATGGAGGATAATTTTGCAGTGGTGCGCCATGCTTATCACTTCTATGAAATCTTCAGGGGTCTTATTGTGGATCTCATTTTTAGTTTCCATGAAGGCTTCGAGAGTCGTGCATTTTTCCATGGCAGATCCGCAGAAGAAACTTTTGGATTGATTGCAATTGAGCTCAACTTTATGTATGAAGCTCTCTTCACCAAGGCTGTTGTGGTGCATTCTAAGCTAGGATGCCTTTTCCGGGCTATATCCTTCACTGCGGTTTTCATAGCCCTTGTTTTCTTCTATAAACTAGAGAAAAAGGCTTTCCACAAGGTCGATGTTGGAATCACCTATACCCTGCTTTATGGTGCCCTATGCCTGGATTCAATAGCCATTTTCTTGGTTATTTTCTCTGAGTGGACTGTCACTGCTATGCACAAGTCTTGGCAAAAATCTTGGGTTGCCAAGATACTTGGAAAGTACCTCAATTTGAAGAAACCAAGGTGGTCTACAGAGTCAACAACTTGCTTGGAGTGGTGTAGACAAATTCTATTTCGAAGGTGGTGCGAATCCATCTCTTCATTCAACTTCATTCACTACTCCCTCAAGGAGCATCGGAAACTGTCCTCTAACATTTTTGACTACTTTGGCATTGGTTACATTGCAATCATCGACCTCTTTGGCCTCAAGGATCTACGAGATAAGATGAAATATCGGACAAGTAGGCCACTGACTAAAGGGTTATGGGAATTCATTTTTAAAGAGTTGAAAGCTAAATCTGTGCTCGCGGATGATCCCGAAACTGCCAAGAGAATATCTACAGCCAGAGGTGATTGGATTCTCCAGGATAGTGAGTGGAATGACGCCCAACATGCTACCTTGCTGTCTTACGTGGTTGATGTTGATTATGACCAGAGCATCCTACTGTGGCACATTGCCACTGAATTATGCTATAACTGTGAGGAGAAAGAAACTTCTCGCTCCGAATCCAGTAAGAATGAAACTTCTCGCCACGAATCCAGTAAGAATGAAACTTCTCGCCACGAATCCAGTAAGAATGAAACTTCTCGCCGCGAATCCAGTAAGAATGAACCTTCTCGCCGTGAAATCAGTAAGACCCTGTCGGATTATATGTTGTATCTCTTAGTTATGCAGCCTAGTTTGACGTCTTCTGTAGCAGGAATCGGACAAATAAGATTCCGGGACACATGCGCAGAAGCGAAGAAATTCTTTAGCAGAAGGGAGCTGAGGAAAGAAGGGGAACAGGCTGAAGAGCTAGTTGCCTGCAAGAGTATGTTGGAGGTAAACACAGATGTTAAACCTGCTGATGTCAAGGGGGATAGAAGTAAATCAGTGTTGTTTGATGCGTGTATTTTAGCGAAGGGGTTGATTAAGATGGAGGATAAGAAATGGGATCTGATGAGTCGAGTATGGGTGGAATTGCTCTCGTATGCCGCATGTCATTGCAGAGCGAAGGATCATGCGCAATTGCTGAGTAAAGGAGGAGAGCTTGTCACTTTTGTTTGGTTACTCATGGCTCATTTCGGCATAGGGGAGCAGTTCCAGATAAACGAGGGTCATGCTAGAGCAAAACTCATTGTCGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

640

Amino Acids

73.64

Weight (kDa)

8.82

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 37 - 303 1.1e-55 Domain of unknown function (DUF4220)
DUF594 PF04578 569 - 620 1.2e-23 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1478
Acc16I TGCGCA 2 cut(s) 1522, 1794
Acc36I ACCTGC 1 cut(s) 1639
AccB1I GGYRCC 1 cut(s) 677
AccI GTMKAC 2 cut(s) 818, 850
AccII CGCG 2 cut(s) 1097, 1383
AciI CCGC 5 cut(s) 465, 587, 1097, 1381, 1764
AclWI GGATC 7 cut(s) 194, 414, 456, 1008, 1097, 1731, 1794
AcsI RAATTY 3 cut(s) 855, 1058, 1535
AcuI CTGAAG 3 cut(s) 62, 373, 1599
AcvI CACGTG 1 cut(s) 143
AcyI GRCGYC 2 cut(s) 1173, 1475
AfaI GTAC 2 cut(s) 236, 791
AfiI CCNNNNNNNGG 3 cut(s) 203, 568, 688
AflII CTTAAG 1 cut(s) 35
AflIII ACRYGT 1 cut(s) 140
AgsI TTSAA 6 cut(s) 175, 243, 696, 802, 895, 1078
AjnI CCWGG 5 cut(s) 112, 202, 302, 687, 1152
AjuI GAANNNNNNNTTGG 2 cut(s) 462, 494
AluBI AGCT 9 cut(s) 162, 223, 499, 518, 553, 1082, 1555, 1585, 1819
AluI AGCT 9 cut(s) 162, 223, 499, 518, 553, 1082, 1555, 1585, 1819
Alw21I GWGCWC 3 cut(s) 74, 501, 1095
Alw44I GTGCAC 1 cut(s) 70
AlwI GGATC 7 cut(s) 194, 414, 456, 1008, 1097, 1731, 1794
AoxI GGCC 2 cut(s) 991, 1034
ApaLI GTGCAC 1 cut(s) 70
ApeKI GCWGC 1 cut(s) 1462
ApoI RAATTY 3 cut(s) 855, 1058, 1535
Asp700I GAANNNNTTC 1 cut(s) 1535
AspLEI GCGC 4 cut(s) 138, 363, 1523, 1795
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 2 cut(s) 569, 1511
AsuHPI GGTGA 4 cut(s) 344, 517, 649, 1151
AsuII TTCGAA 1 cut(s) 865
AvaII GGWCC 1 cut(s) 206
BaeGI GKGCMC 2 cut(s) 74, 682
BanI GGYRCC 1 cut(s) 677
BanII GRGCYC 1 cut(s) 501
BbrPI CACGTG 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 1470
Bbv12I GWGCWC 3 cut(s) 74, 501, 1095
BbvCI CCTCAGC 1 cut(s) 1556
BbvI GCAGC 1 cut(s) 1474
BccI CCATC 2 cut(s) 890, 1699
BceAI ACGGC 1 cut(s) 1395
BcgI CGANNNNNNTGC 6 cut(s) 425, 459, 855, 889, 1871, 1905
BciT130I CCWGG 5 cut(s) 114, 204, 304, 689, 1154
BcnI CCSGG 2 cut(s) 569, 1511
BfaI CTAG 5 cut(s) 554, 620, 1467, 1586, 1893
BfmI CTRYAG 3 cut(s) 819, 1128, 1482
BfrI CTTAAG 1 cut(s) 35
BfuAI ACCTGC 1 cut(s) 1639
BisI GCNGC 3 cut(s) 1381, 1463, 1764
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 3 cut(s) 1382, 1464, 1765
BmcAI AGTACT 1 cut(s) 236
Bme1390I CCNGG 7 cut(s) 114, 204, 304, 569, 689, 1154, 1511
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 2 cut(s) 118, 679
BmrFI CCNGG 7 cut(s) 114, 204, 304, 569, 689, 1154, 1511
BmsI GCATC 5 cut(s) 241, 548, 931, 1236, 1663
BpiI GAAGAC 1 cut(s) 1470
BplI GAGNNNNNCTC 4 cut(s) 221, 253, 1887, 1919
BpmI CTGGAG 1 cut(s) 1136
Bpu10I CCTNAGC 1 cut(s) 1556
Bpu1102I GCTNAGC 1 cut(s) 132
Bpu14I TTCGAA 1 cut(s) 865
BpuEI CTTGAG 2 cut(s) 899, 980
BpuMI CCSGG 2 cut(s) 569, 1511
BsaAI YACGTR 2 cut(s) 143, 1201
BsaHI GRCGYC 2 cut(s) 1173, 1475
BsaJI CCNNGG 3 cut(s) 454, 528, 809
BsaXI ACNNNNNCTCC 2 cut(s) 1806, 1836
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 568, 688
Bse1I ACTGG 4 cut(s) 1299, 1329, 1359, 1389
Bse3DI GCAATG 4 cut(s) 45, 969, 1242, 1771
BseBI CCWGG 5 cut(s) 114, 204, 304, 689, 1154
BseDI CCNNGG 3 cut(s) 454, 528, 809
BseGI GGATG 3 cut(s) 563, 1105, 1227
BseLI CCNNNNNNNGG 3 cut(s) 203, 568, 688
BseMI GCAATG 4 cut(s) 45, 969, 1242, 1771
BseMII CTCAG 4 cut(s) 123, 714, 1547, 1793
BseNI ACTGG 4 cut(s) 1299, 1329, 1359, 1389
BseRI GAGGAG 3 cut(s) 245, 1286, 1827
BseSI GKGCMC 2 cut(s) 74, 682
BseXI GCAGC 1 cut(s) 1474
Bsh1236I CGCG 2 cut(s) 1097, 1383
BshFI GGCC 2 cut(s) 993, 1036
BshNI GGYRCC 1 cut(s) 677
BsiHKAI GWGCWC 3 cut(s) 74, 501, 1095
BsiSI CCGG 2 cut(s) 568, 1510
BslFI GGGAC 1 cut(s) 1526
BslI CCNNNNNNNGG 3 cut(s) 203, 568, 688
BsmFI GGGAC 1 cut(s) 1526
BsmI GAATGC 1 cut(s) 544
BsnI GGCC 2 cut(s) 993, 1036
Bsp119I TTCGAA 1 cut(s) 865
Bsp1286I GDGCHC 4 cut(s) 74, 501, 682, 1095
Bsp143I GATC 7 cut(s) 199, 406, 461, 1000, 1102, 1723, 1786
Bsp1720I GCTNAGC 1 cut(s) 132
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 5 cut(s) 465, 587, 1097, 1381, 1764
BspANI GGCC 2 cut(s) 993, 1036
BspCNI CTCAG 4 cut(s) 124, 715, 1548, 1794
BspFNI CGCG 2 cut(s) 1097, 1383
BspLI GGNNCC 2 cut(s) 118, 679
BspMI ACCTGC 1 cut(s) 1639
BspPI GGATC 7 cut(s) 194, 414, 456, 1008, 1097, 1731, 1794
BspQI GCTCTTC 1 cut(s) 1575
BspT104I TTCGAA 1 cut(s) 865
BspT107I GGYRCC 1 cut(s) 677
BspTI CTTAAG 1 cut(s) 35
BsrDI GCAATG 4 cut(s) 45, 969, 1242, 1771
BsrI ACTGG 4 cut(s) 1299, 1329, 1359, 1389
BssECI CCNNGG 3 cut(s) 454, 528, 809
BssMI GATC 7 cut(s) 199, 406, 461, 1000, 1102, 1723, 1786
BssNI GRCGYC 2 cut(s) 1173, 1475
BssT1I CCWWGG 3 cut(s) 454, 528, 809
Bst2UI CCWGG 5 cut(s) 114, 204, 304, 689, 1154
Bst4CI ACNGT 4 cut(s) 733, 933, 1236, 1268
Bst6I CTCTTC 4 cut(s) 244, 527, 892, 1575
BstACI GRCGYC 2 cut(s) 1173, 1475
BstAFI CTTAAG 1 cut(s) 35
BstBAI YACGTR 2 cut(s) 143, 1201
BstBI TTCGAA 1 cut(s) 865
BstC8I GCNNGC 3 cut(s) 160, 1095, 1594
BstDEI CTNAG 6 cut(s) 132, 549, 723, 1453, 1556, 1802
BstDSI CCRYGG 1 cut(s) 454
BstF5I GGATG 3 cut(s) 563, 1105, 1227
BstFNI CGCG 2 cut(s) 1097, 1383
BstHHI GCGC 4 cut(s) 138, 363, 1523, 1795
BstKTI GATC 7 cut(s) 202, 409, 464, 1003, 1105, 1726, 1789
BstMBI GATC 7 cut(s) 199, 406, 461, 1000, 1102, 1723, 1786
BstMWI GCNNNNNNNGC 2 cut(s) 550, 1181
BstNI CCWGG 5 cut(s) 114, 204, 304, 689, 1154
BstNSI RCATGY 3 cut(s) 1184, 1521, 1770
BstSCI CCNGG 7 cut(s) 112, 202, 302, 567, 687, 1152, 1509
BstSFI CTRYAG 3 cut(s) 819, 1128, 1482
BstSLI GKGCMC 2 cut(s) 74, 682
BstUI CGCG 2 cut(s) 1097, 1383
BstV1I GCAGC 1 cut(s) 1474
BstV2I GAAGAC 1 cut(s) 1470
BstX2I RGATCY 4 cut(s) 406, 461, 1000, 1723
BstXI CCANNNNNNTGG 1 cut(s) 709
BstYI RGATCY 4 cut(s) 406, 461, 1000, 1723
BsuRI GGCC 2 cut(s) 993, 1036
BtgI CCRYGG 1 cut(s) 454
BtsCI GGATG 3 cut(s) 563, 1105, 1227
BtsI GCAGTG 3 cut(s) 360, 582, 735
BtsIMutI CAGTG 6 cut(s) 360, 582, 735, 1037, 1248, 1668
BveI ACCTGC 1 cut(s) 1639
Cac8I GCNNGC 3 cut(s) 160, 1095, 1594
CfoI GCGC 4 cut(s) 138, 363, 1523, 1795
Cfr13I GGNCC 1 cut(s) 206
CseI GACGC 1 cut(s) 1181
Csp6I GTAC 2 cut(s) 235, 790
CviQI GTAC 2 cut(s) 235, 790
DdeI CTNAG 6 cut(s) 132, 549, 723, 1453, 1556, 1802
DpnI GATC 7 cut(s) 201, 408, 463, 1002, 1104, 1725, 1788
DpnII GATC 7 cut(s) 199, 406, 461, 1000, 1102, 1723, 1786
DraI TTTAAA 1 cut(s) 1069
Eam1104I CTCTTC 4 cut(s) 244, 527, 892, 1575
EarI CTCTTC 4 cut(s) 244, 527, 892, 1575
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 3 cut(s) 454, 528, 809
Eco24I GRGCYC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 3 cut(s) 62, 373, 1599
Eco72I CACGTG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 499
EcoRI GAATTC 1 cut(s) 1058
EcoRII CCWGG 5 cut(s) 112, 202, 302, 687, 1152
EcoT14I CCWWGG 3 cut(s) 454, 528, 809
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 3 cut(s) 454, 528, 809
FalI AAGNNNNNCTT 4 cut(s) 167, 199, 1523, 1555
FaqI GGGAC 1 cut(s) 1526
FauNDI CATATG 1 cut(s) 316
FblI GTMKAC 2 cut(s) 818, 850
Fnu4HI GCNGC 3 cut(s) 1381, 1463, 1764
FokI GGATG 3 cut(s) 570, 1112, 1214
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 3 cut(s) 1381, 1463, 1764
FspBI CTAG 5 cut(s) 554, 620, 1467, 1586, 1893
FspI TGCGCA 2 cut(s) 1522, 1794
GlaI GCGC 4 cut(s) 137, 362, 1522, 1794
GluI GCNGC 3 cut(s) 1381, 1463, 1764
GsuI CTGGAG 1 cut(s) 1136
HaeIII GGCC 2 cut(s) 993, 1036
HapII CCGG 2 cut(s) 568, 1510
HgaI GACGC 1 cut(s) 1181
HhaI GCGC 4 cut(s) 138, 363, 1523, 1795
Hin1I GRCGYC 2 cut(s) 1173, 1475
Hin6I GCGC 4 cut(s) 136, 361, 1521, 1793
HinP1I GCGC 4 cut(s) 136, 361, 1521, 1793
HincII GTYRAC 1 cut(s) 828
HindII GTYRAC 1 cut(s) 828
HindIII AAGCTT 1 cut(s) 160
HpaII CCGG 2 cut(s) 568, 1510
HphI GGTGA 4 cut(s) 344, 517, 649, 1151
Hpy166II GTNNAC 7 cut(s) 72, 88, 729, 819, 828, 851, 1615
Hpy188I TCNGA 9 cut(s) 81, 724, 927, 1026, 1294, 1435, 1496, 1728, 1913
Hpy188III TCNNGA 3 cut(s) 436, 1106, 1873
Hpy8I GTNNAC 7 cut(s) 72, 88, 729, 819, 828, 851, 1615
HpyAV CCTTC 9 cut(s) 86, 422, 589, 861, 1413, 1542, 1559, 1684, 1777
HpyCH4III ACNGT 4 cut(s) 733, 933, 1236, 1268
HpyCH4IV ACGT 3 cut(s) 142, 1200, 1475
HpyF10VI GCNNNNNNNGC 2 cut(s) 550, 1181
HpyF3I CTNAG 6 cut(s) 132, 549, 723, 1453, 1556, 1802
HpySE526I ACGT 3 cut(s) 142, 1200, 1475
Hsp92I GRCGYC 2 cut(s) 1173, 1475
HspAI GCGC 4 cut(s) 136, 361, 1521, 1793
Kzo9I GATC 7 cut(s) 199, 406, 461, 1000, 1102, 1723, 1786
LguI GCTCTTC 1 cut(s) 1575
LmnI GCTCC 4 cut(s) 919, 1295, 1552, 1864
Lsp1109I GCAGC 1 cut(s) 1474
LweI GCATC 5 cut(s) 241, 548, 931, 1236, 1663
MaeI CTAG 5 cut(s) 554, 620, 1467, 1586, 1893
MaeII ACGT 3 cut(s) 142, 1200, 1475
MaeIII GTNAC 5 cut(s) 310, 733, 965, 1822, 1836
MalI GATC 7 cut(s) 201, 408, 463, 1002, 1104, 1725, 1788
MboI GATC 7 cut(s) 199, 406, 461, 1000, 1102, 1723, 1786
MfeI CAATTG 2 cut(s) 492, 1796
MflI RGATCY 4 cut(s) 406, 461, 1000, 1723
MhlI GDGCHC 4 cut(s) 74, 501, 682, 1095
MlyI GAGTC 3 cut(s) 448, 833, 1741
MmeI TCCRAC 5 cut(s) 287, 631, 1413, 1587, 1891
MroXI GAANNNNTTC 1 cut(s) 1535
MseI TTAA 8 cut(s) 36, 60, 105, 123, 189, 1068, 1626, 1701
MslI CAYNNNNRTG 2 cut(s) 378, 740
MspCI CTTAAG 1 cut(s) 35
MspI CCGG 2 cut(s) 568, 1510
MspR9I CCNGG 7 cut(s) 114, 204, 304, 569, 689, 1154, 1511
MunI CAATTG 2 cut(s) 492, 1796
Mva1269I GAATGC 1 cut(s) 544
MvaI CCWGG 5 cut(s) 114, 204, 304, 689, 1154
MvnI CGCG 2 cut(s) 1097, 1383
MwoI GCNNNNNNNGC 2 cut(s) 550, 1181
NciI CCSGG 2 cut(s) 569, 1511
NcoI CCATGG 1 cut(s) 454
NdeI CATATG 1 cut(s) 316
NdeII GATC 7 cut(s) 199, 406, 461, 1000, 1102, 1723, 1786
NlaIV GGNNCC 2 cut(s) 118, 679
NmuCI GTSAC 2 cut(s) 733, 1822
NsbI TGCGCA 2 cut(s) 1522, 1794
NspI RCATGY 3 cut(s) 1184, 1521, 1770
NspV TTCGAA 1 cut(s) 865
PciSI GCTCTTC 1 cut(s) 1575
PctI GAATGC 1 cut(s) 544
PdmI GAANNNNTTC 1 cut(s) 1535
PflFI GACNNNGTC 1 cut(s) 1429
PfoI TCCNGGA 5 cut(s) 112, 202, 302, 1152, 1509
PkrI GCNGC 3 cut(s) 1382, 1464, 1765
PleI GAGTC 3 cut(s) 447, 832, 1740
PmaCI CACGTG 1 cut(s) 143
PmlI CACGTG 1 cut(s) 143
PpsI GAGTC 3 cut(s) 447, 832, 1740
Ppu21I YACGTR 2 cut(s) 143, 1201
Psp124BI GAGCTC 1 cut(s) 501
Psp6I CCWGG 5 cut(s) 112, 202, 302, 687, 1152
PspCI CACGTG 1 cut(s) 143
PspGI CCWGG 5 cut(s) 112, 202, 302, 687, 1152
PspN4I GGNNCC 2 cut(s) 118, 679
PspPI GGNCC 1 cut(s) 206
PsuI RGATCY 4 cut(s) 406, 461, 1000, 1723
PsyI GACNNNGTC 1 cut(s) 1429
RsaI GTAC 2 cut(s) 236, 791
RsaNI GTAC 2 cut(s) 235, 790
RseI CAYNNNNRTG 2 cut(s) 378, 740
SacI GAGCTC 1 cut(s) 501
SapI GCTCTTC 1 cut(s) 1575
SaqAI TTAA 8 cut(s) 36, 60, 105, 123, 189, 1068, 1626, 1701
SatI GCNGC 3 cut(s) 1381, 1463, 1764
Sau3AI GATC 7 cut(s) 199, 406, 461, 1000, 1102, 1723, 1786
Sau96I GGNCC 1 cut(s) 206
ScaI AGTACT 1 cut(s) 236
SchI GAGTC 3 cut(s) 448, 833, 1741
ScrFI CCNGG 7 cut(s) 114, 204, 304, 569, 689, 1154, 1511
SduI GDGCHC 4 cut(s) 74, 501, 682, 1095
SfaNI GCATC 5 cut(s) 241, 548, 931, 1236, 1663
SfcI CTRYAG 3 cut(s) 819, 1128, 1482
SfuI TTCGAA 1 cut(s) 865
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 378, 740
SmlI CTYRAG 3 cut(s) 35, 914, 995
SmoI CTYRAG 3 cut(s) 35, 914, 995
SsiI CCGC 5 cut(s) 465, 587, 1097, 1381, 1764
SspMI CTAG 5 cut(s) 554, 620, 1467, 1586, 1893
SstI GAGCTC 1 cut(s) 501
StyD4I CCNGG 7 cut(s) 112, 202, 302, 567, 687, 1152, 1509
StyI CCWWGG 3 cut(s) 454, 528, 809
TaaI ACNGT 4 cut(s) 733, 933, 1236, 1268
TaiI ACGT 3 cut(s) 145, 1203, 1478
TaqI TCGA 5 cut(s) 435, 645, 865, 981, 1735
TatI WGTACW 1 cut(s) 234
TauI GCSGC 2 cut(s) 1383, 1766
Tru1I TTAA 8 cut(s) 36, 60, 105, 123, 189, 1068, 1626, 1701
Tru9I TTAA 8 cut(s) 36, 60, 105, 123, 189, 1068, 1626, 1701
TscAI CASTG 6 cut(s) 360, 589, 742, 1044, 1255, 1668
TseFI GTSAC 2 cut(s) 733, 1822
TseI GCWGC 1 cut(s) 1462
Tsp45I GTSAC 2 cut(s) 733, 1822
TspRI CASTG 6 cut(s) 360, 589, 742, 1044, 1255, 1668
Tth111I GACNNNGTC 1 cut(s) 1429
Vha464I CTTAAG 1 cut(s) 35
VneI GTGCAC 1 cut(s) 70
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 3 cut(s) 855, 1058, 1535
XceI RCATGY 3 cut(s) 1184, 1521, 1770
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XmiI GTMKAC 2 cut(s) 818, 850
XmnI GAANNNNTTC 1 cut(s) 1535
XspI CTAG 5 cut(s) 554, 620, 1467, 1586, 1893
ZraI GACGTC 1 cut(s) 1476
ZrmI AGTACT 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.