RLG00000031322

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000662
Physical Location & Seq
Forward (+)
4081473 .. 4083671
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031322

Sequence Viewer

Length: 2199 bp
ATGGTTTCTCCCATTCCTACCAGTGTCAAGAATATATGGGAAAAGTGGAATCTCCGGGGTTTTGTACTCCTCAGCCTCTCATTGCAAACTATTTTAATTCTTGGTGCACCCTTTCGAAAGCGATCACCTAGCTTATTTGTAACCTTGGTTATCTGGTCATCTTACTTGCTTGCTGACTGGGCAGCTAACTTTGCAATTGGCTTAATTGCAAACAGCCAGGGCGATAATCCATTTATTCCAAACCCCAACAACACCGGTCATTTTAATAGCGATCTTCTGGCATTTTGGGCGCCCTTTCTTCTGTTACACCTTGGTGGCCCAGACACAATTACAGCATTTGCTCTTGAGGATAACACATTGTGGCTAAGGCACTTGTTTGGGCTTGTGTTCCAGGTTATAGCTGCGGTTTATGTCTTCATTCAATCATTTCCCAAAAACAAGCTGTGGCTGCCGACTGTTCTCCTATTTGTAGCAGGAATCATCAAGTATGCCGAGCGCACACGTGCTCTGTATCTTGCAAGCTTGGATAATTTTAAGGAGTCCATGATTAAGAAACCTGATCCTGGTCCCAATTATGCAAAGCTGATGGAGGAATACTCTTCGAAAAAAGATGCCAAGCTTCCAACTCATATAGAGTTGACAGCAGAGCGCAGCAAAGATTCCAGGACTACTACTTATGTGGTGGACCCTGGAGATATGGAACATAATATTGCACTGGTTCGCCATGCTTTTCACTTCTTTAACATCTTCAAGGGTCTTATTGTGGATCTCATTTTCAGCTTTCATGAACGATTTGAGAGCCGTGTGTTTTTCCATGACAGAACAGCAGAGGATGCTTTCAAGTTGGTTGCAATTGAGCTCAATTTCATATATGAAGCTCTATTCACCAAGGTGGTGGTTGTGCATTCTAAGAAGGGATACATTTGCCGAGCTATATCCTTCAGTGCAGTTTGCACAGCTCTTGTTTGCTTCCAAAAAATAGAGAAACATGATTTCCATGGGTTTGATGTTGGAATTACCTATATTTTGCTTTTTGGTGCCTTGGCCTTGGATTCAATAGCTGTGTTCATGCTTATATTCTCTGATTCGACTGTTGCTGCTGTGAAGAAGTCTTGCCAGGATTCTTGTCTTGCCAATATTCTCAAAAGGTACATCACTCTCAAGCAACCTTGGTGGTTCGTTGCCGAAAATGCAAATCCATATTTGGAGAGGTGTAGGCAACTTCTATTTCGAAGGTGGTATGAATCTGTGTCCATATTTAACTTGATACATTATTCCCTCAAGGAATGTCCAAAACCGAGTCCTAATATTTTTGATTACTTCTTCATGGCCTGCATAGAAATCATCTACTTAATGGGTCTTAAGGATCTTCGAGATAAGATCAAATATAGGAGGAGCAAGCCACTCGATAAAGAGCTATGGAAATTCATTTTTGATCAGTTGAAAAGTAAGTCCATGCTCGCAGATGATCCTGAAACTGCCAGGAGAATAAGTTCGGCCAGAGGTGACTGGATTCTACAGGATAGTGACTGGAATAACTATAAAGATTGTCGTAACTTGCTCCGTTTTGTTGTTGATGTTGAATTTGATCAGAGCATTCTACTGTGGCACATTGCTACTGAGTTTTGTTATAATCTTGACATGGATCCAAAAGTTCAGGAGAAGGAACCATACAAGGCTGTAGCTGTTCGGACTGCAATTGAACATTGTAAGATCTTGTCTGATTACATGGTATACCTCTTAGTTATGCAGCCTACTATGATGTCTACTGTGGCAGGAATTGGACAAATAAGATTCCGCGATACGTGTGCAGAAGCCATAAAGTTCTTCAGCAGAAGGGAACTAGGACCAAAAGTCGCTCAAAAAGATGCATGTGAGAGAATTCTTGAGGTGAATACAGATGTTCAGCCGGTTGCTGTTAAAGGGGATCGAAGTAAATCCGTGTTGTTTGATGCATCTATTCTGGCGAAAGAACTGATGACTTTGGATGAAAAGAAATGGAAACTCATGAGTGAAGTGTGGGTGGAACTACTAGCATATGCTGCAGGTCATTGCAGAGCAAATGACCATGTCCAATTGCTGAGCAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTAGTGATGGCTCATTTTGGCATAGGGGAGCAGTTCCAGATAAATGAGGGGCATGCAAGAGCAAAACTCATTGTGGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

733

Amino Acids

84.08

Weight (kDa)

8.54

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 425 1.2e-102 Domain of unknown function (DUF4220)
DUF594 PF04578 663 - 713 3.4e-22 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1632
Acc36I ACCTGC 1 cut(s) 2036
AccB1I GGYRCC 2 cut(s) 289, 1037
AccI GTMKAC 2 cut(s) 1734, 1766
AccII CGCG 1 cut(s) 1800
AciI CCGC 2 cut(s) 404, 1798
AclWI GGATC 7 cut(s) 554, 774, 1374, 1463, 1640, 1653, 1935
AcoI YGGCCR 1 cut(s) 1497
AcsI RAATTY 3 cut(s) 1424, 1583, 1881
AcuI CTGAAG 2 cut(s) 925, 1813
AcvI CACGTG 1 cut(s) 503
AcyI GRCGYC 1 cut(s) 290
AdeI CACNNNGTG 1 cut(s) 360
AfaI GTAC 2 cut(s) 66, 1151
AfiI CCNNNNNNNGG 1 cut(s) 563
AflII CTTAAG 1 cut(s) 1361
AflIII ACRYGT 2 cut(s) 500, 1805
AgeI ACCGGT 1 cut(s) 254
AgsI TTSAA 7 cut(s) 422, 751, 841, 1056, 1444, 1583, 1703
AhdI GACNNNNNGTC 1 cut(s) 1853
AjnI CCWGG 7 cut(s) 216, 390, 562, 662, 688, 1116, 1481
AleI CACNNNNGTG 2 cut(s) 312, 890
AloI GAACNNNNNNTCC 2 cut(s) 1477, 1509
Alw21I GWGCWC 3 cut(s) 109, 508, 861
Alw44I GTGCAC 1 cut(s) 105
AlwI GGATC 7 cut(s) 554, 774, 1374, 1463, 1640, 1653, 1935
AoxI GGCC 4 cut(s) 316, 1044, 1329, 1497
ApaLI GTGCAC 1 cut(s) 105
ApeKI GCWGC 7 cut(s) 182, 401, 448, 651, 1097, 1750, 2042
ApoI RAATTY 3 cut(s) 1424, 1583, 1881
AsiGI ACCGGT 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 1492
AspLEI GCGC 3 cut(s) 292, 498, 651
AspS9I GGNCC 4 cut(s) 317, 566, 685, 1847
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 4 cut(s) 117, 877, 1517, 1903
AsuII TTCGAA 3 cut(s) 115, 602, 1231
AvaII GGWCC 3 cut(s) 566, 685, 1847
BaeGI GKGCMC 1 cut(s) 109
BamHI GGATCC 1 cut(s) 1645
BanI GGYRCC 2 cut(s) 289, 1037
BanII GRGCYC 1 cut(s) 861
BarI GAAGNNNNNNTAC 2 cut(s) 1655, 1687
BbrPI CACGTG 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 3 cut(s) 109, 508, 861
BbvCI CCTCAGC 1 cut(s) 71
BbvI GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1762, 2029
BccI CCATC 2 cut(s) 580, 2116
BceAI ACGGC 1 cut(s) 786
BcgI CGANNNNNNTGC 4 cut(s) 1387, 1421, 1790, 1824
BciT130I CCWGG 7 cut(s) 218, 392, 564, 664, 690, 1118, 1483
BciVI GTATCC 1 cut(s) 911
BclI TGATCA 2 cut(s) 1435, 1588
BcnI CCSGG 1 cut(s) 56
BfaI CTAG 3 cut(s) 129, 1844, 2033
BfmI CTRYAG 3 cut(s) 1517, 1680, 2043
BfoI RGCGCY 1 cut(s) 293
BfrI CTTAAG 1 cut(s) 1361
BfuAI ACCTGC 1 cut(s) 2036
BfuI GTATCC 1 cut(s) 911
BglII AGATCT 1 cut(s) 1713
BisI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1751, 2043
BlpI GCTNAGC 1 cut(s) 2081
BlsI GCNGC 7 cut(s) 184, 403, 450, 653, 1099, 1752, 2044
Bme1390I CCNGG 8 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483
Bme18I GGWCC 3 cut(s) 566, 685, 1847
BmeRI GACNNNNNGTC 1 cut(s) 1853
BmgT120I GGNCC 4 cut(s) 317, 566, 685, 1847
BmiI GGNNCC 6 cut(s) 291, 568, 687, 1039, 1647, 1668
BmrFI CCNGG 8 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 5 cut(s) 601, 823, 1858, 1942, 1964
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 406
BplI GAGNNNNNCTC 4 cut(s) 581, 613, 2166, 2198
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 2 cut(s) 71, 365
Bpu1102I GCTNAGC 1 cut(s) 2081
Bpu14I TTCGAA 3 cut(s) 115, 602, 1231
BpuEI CTTGAG 4 cut(s) 365, 1145, 1265, 1907
BpuMI CCSGG 1 cut(s) 56
BsaAI YACGTR 2 cut(s) 503, 1806
BsaHI GRCGYC 1 cut(s) 290
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 4 cut(s) 1477, 1507, 2085, 2115
Bsc4I CCNNNNNNNGG 1 cut(s) 563
Bse118I RCCGGY 2 cut(s) 254, 1909
Bse1I ACTGG 5 cut(s) 21, 182, 720, 1514, 1535
Bse3DI GCAATG 3 cut(s) 80, 1611, 2050
BseBI CCWGG 7 cut(s) 218, 392, 564, 664, 690, 1118, 1483
BseGI GGATG 2 cut(s) 838, 1993
BseLI CCNNNNNNNGG 1 cut(s) 563
BseMI GCAATG 3 cut(s) 80, 1611, 2050
BseMII CTCAG 3 cut(s) 85, 1611, 2072
BseNI ACTGG 5 cut(s) 21, 182, 720, 1514, 1535
BseRI GAGGAG 2 cut(s) 59, 1408
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1762, 2029
BsgI GTGCAG 2 cut(s) 966, 1830
Bsh1236I CGCG 1 cut(s) 1800
BshFI GGCC 4 cut(s) 318, 1046, 1331, 1499
BshNI GGYRCC 2 cut(s) 289, 1037
BshTI ACCGGT 1 cut(s) 254
BsiHKAI GWGCWC 3 cut(s) 109, 508, 861
BsiSI CCGG 3 cut(s) 55, 255, 1910
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 1 cut(s) 563
BsmFI GGGAC 1 cut(s) 552
BsmI GAATGC 2 cut(s) 904, 1596
BsnI GGCC 4 cut(s) 318, 1046, 1331, 1499
Bsp119I TTCGAA 3 cut(s) 115, 602, 1231
Bsp1286I GDGCHC 3 cut(s) 109, 508, 861
Bsp1720I GCTNAGC 1 cut(s) 2081
Bsp19I CCATGG 1 cut(s) 997
BspACI CCGC 2 cut(s) 404, 1798
BspANI GGCC 4 cut(s) 318, 1046, 1331, 1499
BspCNI CTCAG 3 cut(s) 84, 1612, 2073
BspFNI CGCG 1 cut(s) 1800
BspHI TCATGA 2 cut(s) 784, 2007
BspLI GGNNCC 6 cut(s) 291, 568, 687, 1039, 1647, 1668
BspMAI CTGCAG 1 cut(s) 2047
BspMI ACCTGC 1 cut(s) 2036
BspPI GGATC 7 cut(s) 554, 774, 1374, 1463, 1640, 1653, 1935
BspT104I TTCGAA 3 cut(s) 115, 602, 1231
BspT107I GGYRCC 2 cut(s) 289, 1037
BspTI CTTAAG 1 cut(s) 1361
BsrDI GCAATG 3 cut(s) 80, 1611, 2050
BsrFI RCCGGY 2 cut(s) 254, 1909
BsrI ACTGG 5 cut(s) 21, 182, 720, 1514, 1535
BssAI RCCGGY 2 cut(s) 254, 1909
BssNAI GTATAC 1 cut(s) 1735
BssNI GRCGYC 1 cut(s) 290
BssT1I CCWWGG 7 cut(s) 144, 310, 888, 997, 1041, 1047, 1169
Bst1107I GTATAC 1 cut(s) 1735
Bst2UI CCWGG 7 cut(s) 218, 392, 564, 664, 690, 1118, 1483
Bst4CI ACNGT 4 cut(s) 457, 1093, 1605, 1771
Bst6I CTCTTC 1 cut(s) 604
BstACI GRCGYC 1 cut(s) 290
BstAFI CTTAAG 1 cut(s) 1361
BstAPI GCANNNNNTGC 2 cut(s) 833, 2042
BstBAI YACGTR 2 cut(s) 503, 1806
BstBI TTCGAA 3 cut(s) 115, 602, 1231
BstC8I GCNNGC 6 cut(s) 171, 520, 1333, 1400, 1461, 2169
BstDEI CTNAG 6 cut(s) 71, 365, 909, 1620, 1741, 2081
BstDSI CCRYGG 1 cut(s) 997
BstF5I GGATG 2 cut(s) 838, 1993
BstFNI CGCG 1 cut(s) 1800
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 3 cut(s) 292, 498, 651
BstMWI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 2042
BstNI CCWGG 7 cut(s) 218, 392, 564, 664, 690, 1118, 1483
BstNSI RCATGY 2 cut(s) 1875, 2171
BstSCI CCNGG 8 cut(s) 54, 216, 390, 562, 662, 688, 1116, 1481
BstSFI CTRYAG 3 cut(s) 1517, 1680, 2043
BstSLI GKGCMC 1 cut(s) 109
BstUI CGCG 1 cut(s) 1800
BstV1I GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1762, 2029
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 4 cut(s) 766, 1366, 1645, 1713
BstXI CCANNNNNNTGG 1 cut(s) 895
BstYI RGATCY 4 cut(s) 766, 1366, 1645, 1713
BstZ17I GTATAC 1 cut(s) 1735
BsuI GTATCC 1 cut(s) 911
BsuRI GGCC 4 cut(s) 318, 1046, 1331, 1499
BtgI CCRYGG 1 cut(s) 997
BtsCI GGATG 2 cut(s) 838, 1993
BtsIMutI CAGTG 3 cut(s) 28, 713, 949
BveI ACCTGC 1 cut(s) 2036
Cac8I GCNNGC 6 cut(s) 171, 520, 1333, 1400, 1461, 2169
CciI TCATGA 2 cut(s) 784, 2007
CfoI GCGC 3 cut(s) 292, 498, 651
Cfr10I RCCGGY 2 cut(s) 254, 1909
Cfr13I GGNCC 4 cut(s) 317, 566, 685, 1847
Csp6I GTAC 2 cut(s) 65, 1150
CspAI ACCGGT 1 cut(s) 254
CspCI CAANNNNNGTGG 2 cut(s) 1154, 1189
CviQI GTAC 2 cut(s) 65, 1150
DdeI CTNAG 6 cut(s) 71, 365, 909, 1620, 1741, 2081
DinI GGCGCC 1 cut(s) 291
DraIII CACNNNGTG 1 cut(s) 360
DriI GACNNNNNGTC 1 cut(s) 1853
EaeI YGGCCR 1 cut(s) 1497
Eam1104I CTCTTC 1 cut(s) 604
Eam1105I GACNNNNNGTC 1 cut(s) 1853
EarI CTCTTC 1 cut(s) 604
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 7 cut(s) 144, 310, 888, 997, 1041, 1047, 1169
Eco24I GRGCYC 1 cut(s) 861
Eco47I GGWCC 3 cut(s) 566, 685, 1847
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 2 cut(s) 925, 1813
Eco72I CACGTG 1 cut(s) 503
EcoICRI GAGCTC 1 cut(s) 859
EcoRI GAATTC 1 cut(s) 1881
EcoRII CCWGG 7 cut(s) 216, 390, 562, 662, 688, 1116, 1481
EcoT14I CCWWGG 7 cut(s) 144, 310, 888, 997, 1041, 1047, 1169
EcoT22I ATGCAT 2 cut(s) 1873, 1957
EcoT38I GRGCYC 1 cut(s) 861
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 7 cut(s) 144, 310, 888, 997, 1041, 1047, 1169
FaqI GGGAC 1 cut(s) 552
FauNDI CATATG 1 cut(s) 2038
FbaI TGATCA 2 cut(s) 1435, 1588
FblI GTMKAC 2 cut(s) 1734, 1766
Fnu4HI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1751, 2043
FokI GGATG 2 cut(s) 845, 2000
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1751, 2043
FspBI CTAG 3 cut(s) 129, 1844, 2033
GlaI GCGC 3 cut(s) 291, 497, 650
GluI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1751, 2043
GsuI CTGGAG 1 cut(s) 711
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 4 cut(s) 318, 1046, 1331, 1499
HapII CCGG 3 cut(s) 55, 255, 1910
HhaI GCGC 3 cut(s) 292, 498, 651
Hin1I GRCGYC 1 cut(s) 290
Hin6I GCGC 3 cut(s) 290, 496, 649
HinP1I GCGC 3 cut(s) 290, 496, 649
HincII GTYRAC 1 cut(s) 639
HindII GTYRAC 1 cut(s) 639
HindIII AAGCTT 2 cut(s) 520, 617
HpaII CCGG 3 cut(s) 55, 255, 1910
HphI GGTGA 4 cut(s) 117, 877, 1517, 1903
Hpy166II GTNNAC 5 cut(s) 107, 639, 685, 1735, 1767
Hpy188I TCNGA 4 cut(s) 1084, 1593, 1692, 1723
Hpy8I GTNNAC 5 cut(s) 107, 639, 685, 1735, 1767
HpyAV CCTTC 5 cut(s) 907, 949, 1227, 1657, 1830
HpyCH4III ACNGT 4 cut(s) 457, 1093, 1605, 1771
HpyCH4IV ACGT 2 cut(s) 502, 1805
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 2042
HpyF3I CTNAG 6 cut(s) 71, 365, 909, 1620, 1741, 2081
HpySE526I ACGT 2 cut(s) 502, 1805
Hsp92I GRCGYC 1 cut(s) 290
HspAI GCGC 3 cut(s) 290, 496, 649
KasI GGCGCC 1 cut(s) 289
Ksp22I TGATCA 2 cut(s) 1435, 1588
LmnI GCTCC 3 cut(s) 1395, 1566, 2143
Lsp1109I GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1762, 2029
LweI GCATC 5 cut(s) 601, 823, 1858, 1942, 1964
MaeI CTAG 3 cut(s) 129, 1844, 2033
MaeII ACGT 2 cut(s) 502, 1805
MaeIII GTNAC 6 cut(s) 139, 303, 1505, 1526, 1553, 2101
MboII GAAGA 9 cut(s) 266, 290, 406, 591, 739, 1117, 1315, 1361, 1819
MfeI CAATTG 4 cut(s) 195, 852, 1698, 2075
MflI RGATCY 4 cut(s) 766, 1366, 1645, 1713
MhlI GDGCHC 3 cut(s) 109, 508, 861
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 2 cut(s) 548, 1309
MmeI TCCRAC 2 cut(s) 647, 991
Mph1103I ATGCAT 2 cut(s) 1873, 1957
MroXI GAANNNNTTC 1 cut(s) 1492
MslI CAYNNNNRTG 2 cut(s) 312, 890
MspCI CTTAAG 1 cut(s) 1361
MspI CCGG 3 cut(s) 55, 255, 1910
MspR9I CCNGG 8 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483
MunI CAATTG 4 cut(s) 195, 852, 1698, 2075
Mva1269I GAATGC 2 cut(s) 904, 1596
MvaI CCWGG 7 cut(s) 218, 392, 564, 664, 690, 1118, 1483
MvnI CGCG 1 cut(s) 1800
MwoI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 2042
NarI GGCGCC 1 cut(s) 290
NciI CCSGG 1 cut(s) 56
NcoI CCATGG 1 cut(s) 997
NdeI CATATG 1 cut(s) 2038
NlaIV GGNNCC 6 cut(s) 291, 568, 687, 1039, 1647, 1668
NmeAIII GCCGAG 2 cut(s) 517, 953
NmuCI GTSAC 3 cut(s) 1505, 1526, 2101
NsiI ATGCAT 2 cut(s) 1873, 1957
NspI RCATGY 2 cut(s) 1875, 2171
NspV TTCGAA 3 cut(s) 115, 602, 1231
OliI CACNNNNGTG 2 cut(s) 312, 890
PaeI GCATGC 1 cut(s) 2171
PagI TCATGA 2 cut(s) 784, 2007
PctI GAATGC 2 cut(s) 904, 1596
PdmI GAANNNNTTC 1 cut(s) 1492
PfeI GAWTC 9 cut(s) 49, 477, 659, 1052, 1085, 1121, 1244, 1513, 1794
PflFI GACNNNGTC 1 cut(s) 2069
PfoI TCCNGGA 1 cut(s) 662
PinAI ACCGGT 1 cut(s) 254
PkrI GCNGC 7 cut(s) 184, 403, 450, 653, 1099, 1752, 2044
PleI GAGTC 2 cut(s) 547, 1308
PluTI GGCGCC 1 cut(s) 293
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 2 cut(s) 547, 1308
Ppu21I YACGTR 2 cut(s) 503, 1806
PsiI TTATAA 1 cut(s) 1632
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 7 cut(s) 216, 390, 562, 662, 688, 1116, 1481
PspCI CACGTG 1 cut(s) 503
PspGI CCWGG 7 cut(s) 216, 390, 562, 662, 688, 1116, 1481
PspN4I GGNNCC 6 cut(s) 291, 568, 687, 1039, 1647, 1668
PspPI GGNCC 4 cut(s) 317, 566, 685, 1847
PstI CTGCAG 1 cut(s) 2047
PsuI RGATCY 4 cut(s) 766, 1366, 1645, 1713
PsyI GACNNNGTC 1 cut(s) 2069
RsaI GTAC 2 cut(s) 66, 1151
RsaNI GTAC 2 cut(s) 65, 1150
RseI CAYNNNNRTG 2 cut(s) 312, 890
SacI GAGCTC 1 cut(s) 861
SatI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1751, 2043
Sau96I GGNCC 4 cut(s) 317, 566, 685, 1847
SchI GAGTC 2 cut(s) 548, 1309
ScrFI CCNGG 8 cut(s) 56, 218, 392, 564, 664, 690, 1118, 1483
SduI GDGCHC 3 cut(s) 109, 508, 861
SfaNI GCATC 5 cut(s) 601, 823, 1858, 1942, 1964
SfcI CTRYAG 3 cut(s) 1517, 1680, 2043
SfoI GGCGCC 1 cut(s) 291
SfuI TTCGAA 3 cut(s) 115, 602, 1231
SinI GGWCC 3 cut(s) 566, 685, 1847
SmiMI CAYNNNNRTG 2 cut(s) 312, 890
SmlI CTYRAG 5 cut(s) 344, 1160, 1280, 1361, 1886
SmoI CTYRAG 5 cut(s) 344, 1160, 1280, 1361, 1886
SphI GCATGC 1 cut(s) 2171
SsiI CCGC 2 cut(s) 404, 1798
SspDI GGCGCC 1 cut(s) 289
SspI AATATT 3 cut(s) 709, 1138, 1309
SspMI CTAG 3 cut(s) 129, 1844, 2033
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 8 cut(s) 54, 216, 390, 562, 662, 688, 1116, 1481
StyI CCWWGG 7 cut(s) 144, 310, 888, 997, 1041, 1047, 1169
TaaI ACNGT 4 cut(s) 457, 1093, 1605, 1771
TaiI ACGT 2 cut(s) 505, 1808
TaqI TCGA 7 cut(s) 115, 602, 1088, 1231, 1372, 1407, 1930
TatI WGTACW 1 cut(s) 64
TfiI GAWTC 9 cut(s) 49, 477, 659, 1052, 1085, 1121, 1244, 1513, 1794
TscAI CASTG 3 cut(s) 28, 720, 949
TseFI GTSAC 3 cut(s) 1505, 1526, 2101
TseI GCWGC 7 cut(s) 182, 401, 448, 651, 1097, 1750, 2042
Tsp45I GTSAC 3 cut(s) 1505, 1526, 2101
TspGWI ACGGA 2 cut(s) 1553, 1930
TspRI CASTG 3 cut(s) 28, 720, 949
Tth111I GACNNNGTC 1 cut(s) 2069
Vha464I CTTAAG 1 cut(s) 1361
VneI GTGCAC 1 cut(s) 105
VpaK11BI GGWCC 3 cut(s) 566, 685, 1847
XapI RAATTY 3 cut(s) 1424, 1583, 1881
XceI RCATGY 2 cut(s) 1875, 2171
XmiI GTMKAC 2 cut(s) 1734, 1766
XmnI GAANNNNTTC 1 cut(s) 1492
XspI CTAG 3 cut(s) 129, 1844, 2033
Zsp2I ATGCAT 2 cut(s) 1873, 1957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.