Rmu_sc0004286.1_g000007

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004286.1
OG0000662
Physical Location & Seq
Forward (+)
21745 .. 24078
2334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004286.1_g000007.1.cds

Sequence Viewer

Length: 2334 bp
atgccttcattagccgctcctatttctaacaaggtcaagaatatctgggataagtggaatctgcggagcttcatcatattaagtctctcattgcagaccttcttaattctctttgcacccttgagaaagagaacatcgagcaaatgggtaatcgtgcctatctggttagcttacttgctagctgactgggctgctaactttgccatcggactaattgcaagcagccaaacgaacactgatcaaagttccccagagaatggtaatctcttgacattctgggctccctttcttcttttgcaccttggtggtccagacaccataattgcaattgctatggaagacaatgcgttgtggccaaggcacttgcttggactaatattccaggttgtagctgcaatgtacgtcttcggtatgcagttctctaaccaaaacaagctactgtggccgacactcttgctgtttgtaccaggacttgtcaagtacggcgagcgaacgcgtgctctatatcttgccagcttggaaagtctcaaggaaaccatacttgcaataccggaacctggaccggattatgcaaagtacatggatgagtactcctcaagaaaagctgccggtcttccaattgcaataataaaaggaggaggaggtagctcagattcgaagaatgtgacttaccagctaggagaaggagagactgataatattacactagtggagggtgcttatcacttgtttcaaatttccatgggtcttattatagatcttgtgttcgactatcatcagcgcaatgagagccgtgcttatttcaataaattaacgtcagaacaagcttttacaacaattgcgactgagctcaattttatgtatgaagctctgtacaccaaagcttatgtggtacattcaaaacgggggtacttgttgagagctacatccttcatcgccgtttgcatagcctttgttttcttctataaaatagagaaacatggcttccacaggtttgatgttggaattacttattccttgctttttggtgccatagctcttgattcaatagctctttcaatgctgatattatctgattggacgatcgctgttgtgtacaagttctgcaaaaagtctcgtgcagtcactgatctcataaagaagtacctggagtttcagaaatcatggccacaaactactccaccaaagtgtctggagcggaccagaaggattttatttcgaagatggtgtgaatctgtctccggcttcagcttcatggattattctctccgagaatgtggaagtgtagtccgattccgacctggggaccgttttggccgaattgtttacctgtaccatagattcatcagattcttgggctaccgttaccttcaaatcatcagattctttggcctcaagtatcttcgagatgagatgaagtatagatccagcaagagactggataagaatttgtggaatctcatttttaagcagttgaaagttaaatccgaggaagcaaatgatgttcccgaaaaggccaaacaaatatgttcgtcccaaggggattgggttctccaaaaagagtatcaggagcatccggagtacgagaacttaaaatcgtgctttgttgaaattagtcattttccttatgaccgacgggttctgctgtggcatattgctactgagatgtgctattacggagatgatacaggcaatggtgatgataatcgtaaatatagtaagacattgtctgattatatgctatatctcttaattatgcagcctgctttgatgtccactgtagcaggaatcggacaaaagagatttagagatacggttgcagaagccgataaattctttaaaaaaaggggattgggatccgacgtgaaaataggcgaggcggccgatgtgaaatcaggcgaggtggccgctgtgaaatcaggcgaggtggccgctgtgaaaccgcgcgaggaggccgatgtgaaaccaggcgaagcggcctgtagccaacttcttgatgtaaatacaggagttaaaccagtcgatgtcaaagggaatagaagtaaatctgttttgtttgatgcatgtattttggccaggcagctgatgaaattcaaagaaaagaaatgggaattaataagcttggtgtgggtggaattgctatcatatgctgcaagtcattgcagagcacatagccacgcgcaattgctgactaagggtggagagcttgtcacttttgtttggttactcatggctcgttttggcattgaggaggacatccaagtagaagaggtccaagcaagagtaaaactgatcatggaaaaggggcctgatgaattcaagactcgagagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

777

Amino Acids

88.84

Weight (kDa)

9.05

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1028
AccB7I CCANNNNNTGG 1 cut(s) 257
AccBSI CCGCTC 2 cut(s) 17, 1198
AccII CGCG 4 cut(s) 496, 1945, 1947, 2180
AccIII TCCGGA 1 cut(s) 1576
AciI CCGC 8 cut(s) 15, 64, 1198, 1880, 1908, 1932, 1943, 1976
AclWI GGATC 3 cut(s) 1419, 1851, 1864
AcoI YGGCCR 8 cut(s) 353, 443, 1166, 1315, 1881, 1905, 1929, 2073
AcsI RAATTY 5 cut(s) 735, 1447, 1832, 2090, 2315
AcuI CTGAAG 1 cut(s) 1231
AfiI CCNNNNNNNGG 3 cut(s) 257, 557, 1303
AflIII ACRYGT 1 cut(s) 494
AhlI ACTAGT 1 cut(s) 706
AjiI CACGTC 1 cut(s) 1864
AjnI CCWGG 7 cut(s) 381, 466, 556, 1146, 1300, 1966, 2075
AleI CACNNNNGTG 2 cut(s) 303, 1186
Alw21I GWGCWC 3 cut(s) 502, 852, 2170
Alw26I GTCTC 6 cut(s) 89, 530, 683, 1119, 1243, 1429
AlwI GGATC 3 cut(s) 1419, 1851, 1864
AlwNI CAGNNNCTG 1 cut(s) 1127
Ama87I CYCGRG 1 cut(s) 2325
Aor13HI TCCGGA 1 cut(s) 1576
ApeKI GCWGC 7 cut(s) 191, 222, 392, 605, 1759, 2080, 2150
ApoI RAATTY 5 cut(s) 735, 1447, 1832, 2090, 2315
AseI ATTAAT 1 cut(s) 2114
AspLEI GCGC 3 cut(s) 783, 1947, 2182
AspS9I GGNCC 6 cut(s) 308, 560, 1200, 1306, 2272, 2306
AsuHPI GGTGA 1 cut(s) 1709
AsuII TTCGAA 2 cut(s) 656, 1219
AsuNHI GCTAGC 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 2325
AvaII GGWCC 5 cut(s) 308, 560, 1200, 1306, 2272
BalI TGGCCA 3 cut(s) 355, 1168, 2075
BamHI GGATCC 1 cut(s) 1856
BanI GGYRCC 1 cut(s) 1028
BanII GRGCYC 2 cut(s) 283, 852
BauI CACGAG 1 cut(s) 1116
BbsI GAAGAC 3 cut(s) 345, 397, 605
Bbv12I GWGCWC 3 cut(s) 502, 852, 2170
BbvI GCAGC 7 cut(s) 178, 234, 379, 592, 1771, 2092, 2137
BccI CCATC 2 cut(s) 212, 1218
BceAI ACGGC 3 cut(s) 499, 777, 923
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BciT130I CCWGG 7 cut(s) 383, 468, 558, 1148, 1302, 1968, 2077
BclI TGATCA 2 cut(s) 238, 2292
BcoDI GTCTC 6 cut(s) 89, 530, 683, 1119, 1243, 1429
BcuI ACTAGT 1 cut(s) 706
BfaI CTAG 3 cut(s) 179, 677, 707
BfmI CTRYAG 2 cut(s) 1779, 1981
BglII AGATCT 1 cut(s) 757
BmcAI AGTACT 1 cut(s) 590
Bme1390I CCNGG 7 cut(s) 383, 468, 558, 1148, 1302, 1968, 2077
Bme18I GGWCC 5 cut(s) 308, 560, 1200, 1306, 2272
BmeT110I CYCGRG 1 cut(s) 2325
BmgBI CACGTC 1 cut(s) 1864
BmgT120I GGNCC 6 cut(s) 308, 560, 1200, 1306, 2272, 2306
BmiI GGNNCC 6 cut(s) 282, 555, 1030, 1307, 1858, 2307
BmrFI CCNGG 7 cut(s) 383, 468, 558, 1148, 1302, 1968, 2077
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 2 cut(s) 1582, 2050
BmtI GCTAGC 1 cut(s) 182
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 3 cut(s) 345, 397, 605
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 2 cut(s) 1169, 1214
Bpu14I TTCGAA 2 cut(s) 656, 1219
BpuEI CTTGAG 4 cut(s) 142, 512, 580, 1379
BsaBI GATNNNNATC 2 cut(s) 1704, 1855
BsaJI CCNNGG 6 cut(s) 301, 356, 741, 1301, 1488, 1537
BsaWI WCCGGW 3 cut(s) 550, 562, 1576
BsaXI ACNNNNNCTCC 2 cut(s) 2193, 2223
Bsc4I CCNNNNNNNGG 3 cut(s) 257, 557, 1303
Bse118I RCCGGY 1 cut(s) 608
Bse1I ACTGG 3 cut(s) 191, 1443, 2018
Bse3DI GCAATG 5 cut(s) 89, 402, 790, 1699, 2158
Bse8I GATNNNNATC 2 cut(s) 1704, 1855
BseAI TCCGGA 1 cut(s) 1576
BseBI CCWGG 7 cut(s) 383, 468, 558, 1148, 1302, 1968, 2077
BseDI CCNNGG 6 cut(s) 301, 356, 741, 1301, 1488, 1537
BseGI GGATG 4 cut(s) 589, 926, 1573, 2256
BseJI GATNNNNATC 2 cut(s) 1704, 1855
BseLI CCNNNNNNNGG 3 cut(s) 257, 557, 1303
BseMI GCAATG 5 cut(s) 89, 402, 790, 1699, 2158
BseMII CTCAG 3 cut(s) 663, 837, 1653
BseNI ACTGG 3 cut(s) 191, 1443, 2018
BseRI GAGGAG 5 cut(s) 583, 651, 654, 1964, 2264
BseX3I CGGCCG 1 cut(s) 1881
BseXI GCAGC 7 cut(s) 178, 234, 379, 592, 1771, 2092, 2137
BsgI GTGCAG 1 cut(s) 1140
Bsh1236I CGCG 4 cut(s) 496, 1945, 1947, 2180
Bsh1285I CGRYCG 2 cut(s) 1086, 1884
BshNI GGYRCC 1 cut(s) 1028
BsiEI CGRYCG 2 cut(s) 1086, 1884
BsiHKAI GWGCWC 3 cut(s) 502, 852, 2170
BsiHKCI CYCGRG 1 cut(s) 2325
BsiSI CCGG 5 cut(s) 551, 563, 609, 1242, 1577
BslFI GGGAC 2 cut(s) 1319, 1519
BslI CCNNNNNNNGG 3 cut(s) 257, 557, 1303
BsmAI GTCTC 6 cut(s) 89, 530, 683, 1119, 1243, 1429
BsmFI GGGAC 2 cut(s) 1319, 1519
BsoBI CYCGRG 1 cut(s) 2325
Bsp119I TTCGAA 2 cut(s) 656, 1219
Bsp1286I GDGCHC 4 cut(s) 283, 502, 852, 2170
Bsp13I TCCGGA 1 cut(s) 1576
Bsp1407I TGTACA 2 cut(s) 873, 1095
Bsp143I GATC 7 cut(s) 238, 757, 1083, 1129, 1424, 1856, 2292
Bsp19I CCATGG 1 cut(s) 741
BspACI CCGC 8 cut(s) 15, 64, 1198, 1880, 1908, 1932, 1943, 1976
BspCNI CTCAG 3 cut(s) 662, 838, 1654
BspEI TCCGGA 1 cut(s) 1576
BspFNI CGCG 4 cut(s) 496, 1945, 1947, 2180
BspLI GGNNCC 6 cut(s) 282, 555, 1030, 1307, 1858, 2307
BspOI GCTAGC 1 cut(s) 182
BspPI GGATC 3 cut(s) 1419, 1851, 1864
BspT104I TTCGAA 2 cut(s) 656, 1219
BspT107I GGYRCC 1 cut(s) 1028
BsrBI CCGCTC 2 cut(s) 17, 1198
BsrDI GCAATG 5 cut(s) 89, 402, 790, 1699, 2158
BsrFI RCCGGY 1 cut(s) 608
BsrGI TGTACA 2 cut(s) 873, 1095
BsrI ACTGG 3 cut(s) 191, 1443, 2018
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 6 cut(s) 301, 356, 741, 1301, 1488, 1537
BssMI GATC 7 cut(s) 238, 757, 1083, 1129, 1424, 1856, 2292
BssSI CACGAG 1 cut(s) 1116
BssT1I CCWWGG 4 cut(s) 301, 356, 741, 1537
Bst2BI CACGAG 1 cut(s) 1116
Bst2UI CCWGG 7 cut(s) 383, 468, 558, 1148, 1302, 1968, 2077
Bst4CI ACNGT 5 cut(s) 441, 1310, 1364, 1780, 1816
Bst6I CTCTTC 1 cut(s) 2262
BstAUI TGTACA 2 cut(s) 873, 1095
BstBI TTCGAA 2 cut(s) 656, 1219
BstC8I GCNNGC 6 cut(s) 180, 220, 488, 498, 514, 1764
BstDEI CTNAG 4 cut(s) 649, 846, 1662, 2193
BstDSI CCRYGG 1 cut(s) 741
BstF5I GGATG 4 cut(s) 589, 926, 1573, 2256
BstFNI CGCG 4 cut(s) 496, 1945, 1947, 2180
BstHHI GCGC 3 cut(s) 783, 1947, 2182
BstKTI GATC 7 cut(s) 241, 760, 1086, 1132, 1427, 1859, 2295
BstMAI GTCTC 6 cut(s) 89, 530, 683, 1119, 1243, 1429
BstMBI GATC 7 cut(s) 238, 757, 1083, 1129, 1424, 1856, 2292
BstMCI CGRYCG 2 cut(s) 1086, 1884
BstMWI GCNNNNNNNGC 4 cut(s) 188, 200, 442, 789
BstNI CCWGG 7 cut(s) 383, 468, 558, 1148, 1302, 1968, 2077
BstNSI RCATGY 1 cut(s) 2067
BstSCI CCNGG 7 cut(s) 381, 466, 556, 1146, 1300, 1966, 2075
BstSFI CTRYAG 2 cut(s) 1779, 1981
BstUI CGCG 4 cut(s) 496, 1945, 1947, 2180
BstV1I GCAGC 7 cut(s) 178, 234, 379, 592, 1771, 2092, 2137
BstV2I GAAGAC 3 cut(s) 345, 397, 605
BstX2I RGATCY 3 cut(s) 757, 1424, 1856
BstYI RGATCY 3 cut(s) 757, 1424, 1856
BstZI CGGCCG 1 cut(s) 1881
BtgI CCRYGG 1 cut(s) 741
BtgZI GCGATG 1 cut(s) 919
BtrI CACGTC 1 cut(s) 1864
BtsCI GGATG 4 cut(s) 589, 926, 1573, 2256
BtsIMutI CAGTG 3 cut(s) 234, 1125, 1776
Cac8I GCNNGC 6 cut(s) 180, 220, 488, 498, 514, 1764
CaiI CAGNNNCTG 1 cut(s) 1127
CfoI GCGC 3 cut(s) 783, 1947, 2182
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 6 cut(s) 308, 560, 1200, 1306, 2272, 2306
CviAII CATG 8 cut(s) 580, 742, 980, 1164, 1255, 2064, 2230, 2296
DdeI CTNAG 4 cut(s) 649, 846, 1662, 2193
DpnI GATC 7 cut(s) 240, 759, 1085, 1131, 1426, 1858, 2294
DpnII GATC 7 cut(s) 238, 757, 1083, 1129, 1424, 1856, 2292
DraI TTTAAA 1 cut(s) 1840
EaeI YGGCCR 8 cut(s) 353, 443, 1166, 1315, 1881, 1905, 1929, 2073
EagI CGGCCG 1 cut(s) 1881
Eam1104I CTCTTC 1 cut(s) 2262
EarI CTCTTC 1 cut(s) 2262
Ecl136II GAGCTC 1 cut(s) 850
EclXI CGGCCG 1 cut(s) 1881
Eco130I CCWWGG 4 cut(s) 301, 356, 741, 1537
Eco24I GRGCYC 2 cut(s) 283, 852
Eco47I GGWCC 5 cut(s) 308, 560, 1200, 1306, 2272
Eco52I CGGCCG 1 cut(s) 1881
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 1 cut(s) 1231
Eco88I CYCGRG 1 cut(s) 2325
EcoICRI GAGCTC 1 cut(s) 850
EcoO109I RGGNCCY 1 cut(s) 2306
EcoRI GAATTC 1 cut(s) 2315
EcoRII CCWGG 7 cut(s) 381, 466, 556, 1146, 1300, 1966, 2075
EcoT14I CCWWGG 4 cut(s) 301, 356, 741, 1537
EcoT22I ATGCAT 1 cut(s) 2065
EcoT38I GRGCYC 2 cut(s) 283, 852
ErhI CCWWGG 4 cut(s) 301, 356, 741, 1537
FaeI CATG 8 cut(s) 583, 745, 983, 1167, 1258, 2067, 2233, 2299
FaqI GGGAC 2 cut(s) 1319, 1519
FatI CATG 8 cut(s) 579, 741, 979, 1163, 1254, 2063, 2229, 2295
FauNDI CATATG 1 cut(s) 2146
FbaI TGATCA 2 cut(s) 238, 2292
FokI GGATG 4 cut(s) 596, 913, 1560, 2243
FriOI GRGCYC 2 cut(s) 283, 852
FspBI CTAG 3 cut(s) 179, 677, 707
GlaI GCGC 3 cut(s) 782, 1946, 2181
GsuI CTGGAG 2 cut(s) 1169, 1214
HapII CCGG 5 cut(s) 551, 563, 609, 1242, 1577
HhaI GCGC 3 cut(s) 783, 1947, 2182
Hin1II CATG 8 cut(s) 583, 745, 983, 1167, 1258, 2067, 2233, 2299
Hin6I GCGC 3 cut(s) 781, 1945, 2180
HinP1I GCGC 3 cut(s) 781, 1945, 2180
HindIII AAGCTT 3 cut(s) 825, 882, 2119
HpaII CCGG 5 cut(s) 551, 563, 609, 1242, 1577
HphI GGTGA 1 cut(s) 1709
Hpy166II GTNNAC 4 cut(s) 876, 1096, 1327, 1776
Hpy8I GTNNAC 4 cut(s) 876, 1096, 1327, 1776
Hpy99I CGWCG 2 cut(s) 1638, 1865
HpyAV CCTTC 6 cut(s) 15, 109, 677, 940, 1200, 1379
HpyCH4III ACNGT 5 cut(s) 441, 1310, 1364, 1780, 1816
HpyCH4IV ACGT 3 cut(s) 402, 815, 1863
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 200, 442, 789
HpyF3I CTNAG 4 cut(s) 649, 846, 1662, 2193
HpySE526I ACGT 3 cut(s) 402, 815, 1863
Hsp92II CATG 8 cut(s) 583, 745, 983, 1167, 1258, 2067, 2233, 2299
HspAI GCGC 3 cut(s) 781, 1945, 2180
Kpn2I TCCGGA 1 cut(s) 1576
Ksp22I TGATCA 2 cut(s) 238, 2292
Kzo9I GATC 7 cut(s) 238, 757, 1083, 1129, 1424, 1856, 2292
LmnI GCTCC 5 cut(s) 22, 66, 286, 1195, 1570
Lsp1109I GCAGC 7 cut(s) 178, 234, 379, 592, 1771, 2092, 2137
LweI GCATC 2 cut(s) 1582, 2050
MaeI CTAG 3 cut(s) 179, 677, 707
MaeII ACGT 3 cut(s) 402, 815, 1863
MaeIII GTNAC 5 cut(s) 664, 1123, 1364, 2209, 2223
MalI GATC 7 cut(s) 240, 759, 1085, 1131, 1426, 1858, 2294
MbiI CCGCTC 2 cut(s) 17, 1198
MboI GATC 7 cut(s) 238, 757, 1083, 1129, 1424, 1856, 2292
MboII GAAGA 9 cut(s) 281, 350, 397, 605, 670, 952, 1233, 1394, 2279
MfeI CAATTG 4 cut(s) 327, 618, 837, 2183
MflI RGATCY 3 cut(s) 757, 1424, 1856
MhlI GDGCHC 4 cut(s) 283, 502, 852, 2170
MlsI TGGCCA 3 cut(s) 355, 1168, 2075
MluI ACGCGT 1 cut(s) 494
MluNI TGGCCA 3 cut(s) 355, 1168, 2075
MlyI GAGTC 1 cut(s) 2317
MmeI TCCRAC 3 cut(s) 982, 1321, 1884
Mox20I TGGCCA 3 cut(s) 355, 1168, 2075
Mph1103I ATGCAT 1 cut(s) 2065
MroI TCCGGA 1 cut(s) 1576
MscI TGGCCA 3 cut(s) 355, 1168, 2075
MslI CAYNNNNRTG 2 cut(s) 303, 1186
Msp20I TGGCCA 3 cut(s) 355, 1168, 2075
MspA1I CMGCKG 3 cut(s) 1910, 1934, 2083
MspI CCGG 5 cut(s) 551, 563, 609, 1242, 1577
MspR9I CCNGG 7 cut(s) 383, 468, 558, 1148, 1302, 1968, 2077
MunI CAATTG 4 cut(s) 327, 618, 837, 2183
MvaI CCWGG 7 cut(s) 383, 468, 558, 1148, 1302, 1968, 2077
MvnI CGCG 4 cut(s) 496, 1945, 1947, 2180
MwoI GCNNNNNNNGC 4 cut(s) 188, 200, 442, 789
NcoI CCATGG 1 cut(s) 741
NdeI CATATG 1 cut(s) 2146
NdeII GATC 7 cut(s) 238, 757, 1083, 1129, 1424, 1856, 2292
NheI GCTAGC 1 cut(s) 178
NlaIII CATG 8 cut(s) 583, 745, 983, 1167, 1258, 2067, 2233, 2299
NlaIV GGNNCC 6 cut(s) 282, 555, 1030, 1307, 1858, 2307
NmuCI GTSAC 3 cut(s) 664, 1123, 2209
NsiI ATGCAT 1 cut(s) 2065
NspI RCATGY 1 cut(s) 2067
NspV TTCGAA 2 cut(s) 656, 1219
OliI CACNNNNGTG 2 cut(s) 303, 1186
PaeR7I CTCGAG 1 cut(s) 2325
PflFI GACNNNGTC 1 cut(s) 1726
PflMI CCANNNNNTGG 1 cut(s) 257
Ple19I CGATCG 1 cut(s) 1086
PleI GAGTC 1 cut(s) 2317
PpsI GAGTC 1 cut(s) 2317
PshBI ATTAAT 1 cut(s) 2114
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 7 cut(s) 381, 466, 556, 1146, 1300, 1966, 2075
PspGI CCWGG 7 cut(s) 381, 466, 556, 1146, 1300, 1966, 2075
PspN4I GGNNCC 6 cut(s) 282, 555, 1030, 1307, 1858, 2307
PspPI GGNCC 6 cut(s) 308, 560, 1200, 1306, 2272, 2306
PstNI CAGNNNCTG 1 cut(s) 1127
PsuI RGATCY 3 cut(s) 757, 1424, 1856
PsyI GACNNNGTC 1 cut(s) 1726
PvuI CGATCG 1 cut(s) 1086
PvuII CAGCTG 1 cut(s) 2083
RseI CAYNNNNRTG 2 cut(s) 303, 1186
SacI GAGCTC 1 cut(s) 852
Sau3AI GATC 7 cut(s) 238, 757, 1083, 1129, 1424, 1856, 2292
Sau96I GGNCC 6 cut(s) 308, 560, 1200, 1306, 2272, 2306
ScaI AGTACT 1 cut(s) 590
SchI GAGTC 1 cut(s) 2317
ScrFI CCNGG 7 cut(s) 383, 468, 558, 1148, 1302, 1968, 2077
SduI GDGCHC 4 cut(s) 283, 502, 852, 2170
SfaNI GCATC 2 cut(s) 1582, 2050
SfcI CTRYAG 2 cut(s) 1779, 1981
Sfr274I CTCGAG 1 cut(s) 2325
SfuI TTCGAA 2 cut(s) 656, 1219
SinI GGWCC 5 cut(s) 308, 560, 1200, 1306, 2272
SlaI CTCGAG 1 cut(s) 2325
SmiMI CAYNNNNRTG 2 cut(s) 303, 1186
SmlI CTYRAG 5 cut(s) 121, 527, 595, 1394, 2325
SmoI CTYRAG 5 cut(s) 121, 527, 595, 1394, 2325
SpeI ACTAGT 1 cut(s) 706
SsiI CCGC 8 cut(s) 15, 64, 1198, 1880, 1908, 1932, 1943, 1976
SspI AATATT 2 cut(s) 378, 700
SspMI CTAG 3 cut(s) 179, 677, 707
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 7 cut(s) 381, 466, 556, 1146, 1300, 1966, 2075
StyI CCWWGG 4 cut(s) 301, 356, 741, 1537
TaaI ACNGT 5 cut(s) 441, 1310, 1364, 1780, 1816
TaiI ACGT 3 cut(s) 405, 818, 1866
TaqI TCGA 7 cut(s) 137, 656, 768, 1219, 1405, 2022, 2326
TaqII GACCGA 1 cut(s) 1647
TatI WGTACW 4 cut(s) 576, 588, 873, 1095
TauI GCSGC 5 cut(s) 17, 1883, 1910, 1934, 1979
TscAI CASTG 3 cut(s) 241, 1132, 1783
TseFI GTSAC 3 cut(s) 664, 1123, 2209
TseI GCWGC 7 cut(s) 191, 222, 392, 605, 1759, 2080, 2150
Tsp45I GTSAC 3 cut(s) 664, 1123, 2209
TspDTI ATGAA 8 cut(s) 61, 879, 922, 1243, 1333, 1430, 2102, 2328
TspGWI ACGGA 1 cut(s) 1692
TspRI CASTG 3 cut(s) 241, 1132, 1783
Tth111I GACNNNGTC 1 cut(s) 1726
Van91I CCANNNNNTGG 1 cut(s) 257
VpaK11BI GGWCC 5 cut(s) 308, 560, 1200, 1306, 2272
VspI ATTAAT 1 cut(s) 2114
XapI RAATTY 5 cut(s) 735, 1447, 1832, 2090, 2315
XceI RCATGY 1 cut(s) 2067
XcmI CCANNNNNNNNNTGG 2 cut(s) 886, 1435
XhoI CTCGAG 1 cut(s) 2325
XspI CTAG 3 cut(s) 179, 677, 707
ZrmI AGTACT 1 cut(s) 590
Zsp2I ATGCAT 1 cut(s) 2065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.