pycom17g13040

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000662
Physical Location & Seq
Forward (+)
10717800 .. 10719173
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g13040.1

Sequence Viewer

Length: 1209 bp
ATGTATGTACTTTCGCAATTGAACCTCAGGGGCTTTATCCTCTTAAGCCTCACATTGCAGACCCTTTTAATTCTTTTTGCACCCGCGCGAAAGCGGACGCCAAACATGGGGATAATGTTCATCGTTTGGTCATCTTACTTGCTCGCTGACTGGGCTGCTAACTTTGCAATTGGATTAATCTCAAACAGCCAAGGCGACCATGCAGGTGACGACTATCCTGGTGATCTTCTGGCATTTTGGGCTCCCTTTCTTTTGTTGCACCTTGGCGGTCCAGACACCATTACTGCGTTTGCGCTTGAAGATAACACTCTGTGGCTCAGGCACTTTCTTGGCCTCATATTCCAAGTTGCAGCTGCTGTGTATGTCTTCATTCGATCCTTTCCCAAAAATAAACTCTGGCTCCCAACCGGCCTCCTTTTTCTTGCTGGAATCATCAAGTATGCTGAGCGCACACGGTCCTTGTATAGTGCAAGCTTGGATAATTTCGAGGAAGCCATGATAAAAGAAGCTGATCCAGGACCGAATTACGCGAAGCTTATGGAAGAGTACTCCTCGAGAAAAGATTCCCAACTTCCAACTCACATGGAACTCACAGCAGAACGCAGCAAGGAATCTAGGACGGTGACTTACGTGATAGGCAAAGATGAGCTGGACAGTGATATTGCAGTGGTGAAACATGCTCGTTATTTCTATCAAATCTTCAAGGGTCTAATTGTCGATCTCATTTTCAGCTTCCATGAACGCCAGGAGAGTCGAGCAGTCTTCCATGAAAGAACACCGAAAGAAGCATTTAAATTAATTGCAGCTGAGCTCAACTTTATGTATGAAGCTTTCGTCACCAAGGCTGCTGTGGTGCATACTAAGCCAGCATACATTCTCCGGGTGGTTTCCTTCAGTTTTTGGCAAAATTCTCGTCTAGCTGAAACTATTTTCGGAAAGTATCTCGATTTCAAGAGATCGTGGTGGTGGCCTACGGCAAAAACAAGAAATGAGGGCGGCCACATTCCTAAAACAAAAGCAACGAAATGTTTGGAGTGGATTAGGAGAATCCTATTTCGGAGGTCGTATGAATCTGTCTCTACATTCAACTTGATACATTACTCCCTCAAGGAGAAGCGGAAAATGTCCTCGATATGGTATGCTACTTTGGCATTGGCTACATTAAAGTCATCACCTTATTTGGCCTCAAGGATCTTCATGATAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

403

Amino Acids

46.33

Weight (kDa)

9.49

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 43 - 301 1.5e-78 Domain of unknown function (DUF4220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 194
AccII CGCG 3 cut(s) 86, 88, 530
AciI CCGC 5 cut(s) 84, 94, 267, 996, 1117
AclWI GGATC 3 cut(s) 369, 506, 1199
AcoI YGGCCR 1 cut(s) 997
AcsI RAATTY 1 cut(s) 907
AcuI CTGAAG 1 cut(s) 877
AcyI GRCGYC 1 cut(s) 98
AdeI CACNNNGTG 1 cut(s) 312
AfaI GTAC 2 cut(s) 9, 548
AfiI CCNNNNNNNGG 2 cut(s) 107, 1134
AflII CTTAAG 1 cut(s) 43
AgsI TTSAA 5 cut(s) 22, 299, 703, 952, 1087
AjnI CCWGG 3 cut(s) 217, 514, 744
Alw21I GWGCWC 1 cut(s) 813
Alw26I GTCTC 1 cut(s) 1081
AlwI GGATC 3 cut(s) 369, 506, 1199
AlwNI CAGNNNCTG 1 cut(s) 356
Ama87I CYCGRG 1 cut(s) 553
AoxI GGCC 5 cut(s) 331, 409, 968, 997, 1182
ApeKI GCWGC 6 cut(s) 155, 350, 353, 603, 803, 845
ApoI RAATTY 1 cut(s) 907
ArsI GACNNNNNNTTYG 2 cut(s) 898, 930
AseI ATTAAT 2 cut(s) 176, 797
Asp700I GAANNNNTTC 1 cut(s) 562
AspLEI GCGC 3 cut(s) 88, 295, 450
AspS9I GGNCC 3 cut(s) 269, 456, 518
AsuC2I CCSGG 1 cut(s) 881
AsuHPI GGTGA 6 cut(s) 218, 233, 634, 682, 829, 1164
AvaI CYCGRG 1 cut(s) 553
AvaII GGWCC 3 cut(s) 269, 456, 518
AxyI CCTNAGG 1 cut(s) 26
BanII GRGCYC 2 cut(s) 244, 813
BbsI GAAGAC 2 cut(s) 358, 754
Bbv12I GWGCWC 1 cut(s) 813
BbvI GCAGC 6 cut(s) 142, 340, 362, 615, 815, 832
BceAI ACGGC 1 cut(s) 990
BcgI CGANNNNNNTGC 2 cut(s) 893, 927
BciT130I CCWGG 3 cut(s) 219, 516, 746
BcnI CCSGG 1 cut(s) 881
BcoDI GTCTC 1 cut(s) 1081
BfaI CTAG 2 cut(s) 615, 917
BfrI CTTAAG 1 cut(s) 43
BfuAI ACCTGC 1 cut(s) 194
BisI GCNGC 7 cut(s) 156, 351, 354, 604, 804, 846, 997
BlpI GCTNAGC 2 cut(s) 444, 807
BlsI GCNGC 7 cut(s) 157, 352, 355, 605, 805, 847, 998
BmcAI AGTACT 1 cut(s) 548
Bme1390I CCNGG 4 cut(s) 219, 516, 746, 881
Bme18I GGWCC 3 cut(s) 269, 456, 518
BmeT110I CYCGRG 1 cut(s) 553
BmgT120I GGNCC 3 cut(s) 269, 456, 518
BmiI GGNNCC 2 cut(s) 243, 401
BmrFI CCNGG 4 cut(s) 219, 516, 746, 881
BmrI ACTGGG 1 cut(s) 160
BmuI ACTGGG 1 cut(s) 160
BpiI GAAGAC 2 cut(s) 358, 754
BplI GAGNNNNNCTC 2 cut(s) 536, 568
Bpu10I CCTNAGC 1 cut(s) 317
Bpu1102I GCTNAGC 2 cut(s) 444, 807
BpuEI CTTGAG 2 cut(s) 1091, 1171
BpuMI CCSGG 1 cut(s) 881
BsaAI YACGTR 1 cut(s) 631
BsaHI GRCGYC 1 cut(s) 98
BsaJI CCNNGG 3 cut(s) 190, 262, 840
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 1134
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 1 cut(s) 155
Bse21I CCTNAGG 1 cut(s) 26
Bse3DI GCAATG 1 cut(s) 53
BseBI CCWGG 3 cut(s) 219, 516, 746
BseDI CCNNGG 3 cut(s) 190, 262, 840
BseLI CCNNNNNNNGG 2 cut(s) 107, 1134
BseMI GCAATG 1 cut(s) 53
BseMII CTCAG 4 cut(s) 40, 331, 435, 798
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 541
BseXI GCAGC 6 cut(s) 142, 340, 362, 615, 815, 832
Bsh1236I CGCG 3 cut(s) 86, 88, 530
BshFI GGCC 5 cut(s) 333, 411, 970, 999, 1184
BsiHKAI GWGCWC 1 cut(s) 813
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 2 cut(s) 408, 880
BslI CCNNNNNNNGG 2 cut(s) 107, 1134
BsmAI GTCTC 1 cut(s) 1081
BsnI GGCC 5 cut(s) 333, 411, 970, 999, 1184
BsoBI CYCGRG 1 cut(s) 553
Bsp1286I GDGCHC 2 cut(s) 244, 813
Bsp143I GATC 6 cut(s) 223, 374, 511, 718, 956, 1191
Bsp1720I GCTNAGC 2 cut(s) 444, 807
BspACI CCGC 5 cut(s) 84, 94, 267, 996, 1117
BspANI GGCC 5 cut(s) 333, 411, 970, 999, 1184
BspCNI CTCAG 4 cut(s) 39, 330, 436, 799
BspFNI CGCG 3 cut(s) 86, 88, 530
BspHI TCATGA 1 cut(s) 1197
BspLI GGNNCC 2 cut(s) 243, 401
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 3 cut(s) 369, 506, 1199
BspTI CTTAAG 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 53
BsrFI RCCGGY 1 cut(s) 407
BsrI ACTGG 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 3 cut(s) 190, 262, 840
BssMI GATC 6 cut(s) 223, 374, 511, 718, 956, 1191
BssNI GRCGYC 1 cut(s) 98
BssT1I CCWWGG 3 cut(s) 190, 262, 840
Bst2UI CCWGG 3 cut(s) 219, 516, 746
Bst4CI ACNGT 3 cut(s) 456, 622, 656
Bst6I CTCTTC 1 cut(s) 537
BstACI GRCGYC 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 43
BstBAI YACGTR 1 cut(s) 631
BstC8I GCNNGC 3 cut(s) 144, 472, 867
BstDEI CTNAG 5 cut(s) 26, 317, 444, 807, 861
BstFNI CGCG 3 cut(s) 86, 88, 530
BstHHI GCGC 3 cut(s) 88, 295, 450
BstKTI GATC 6 cut(s) 226, 377, 514, 721, 959, 1194
BstMAI GTCTC 1 cut(s) 1081
BstMBI GATC 6 cut(s) 223, 374, 511, 718, 956, 1191
BstMWI GCNNNNNNNGC 5 cut(s) 152, 164, 239, 862, 1148
BstNI CCWGG 3 cut(s) 219, 516, 746
BstNSI RCATGY 1 cut(s) 680
BstSCI CCNGG 4 cut(s) 217, 514, 744, 879
BstUI CGCG 3 cut(s) 86, 88, 530
BstV1I GCAGC 6 cut(s) 142, 340, 362, 615, 815, 832
BstV2I GAAGAC 2 cut(s) 358, 754
BstX2I RGATCY 1 cut(s) 1191
BstYI RGATCY 1 cut(s) 1191
Bsu36I CCTNAGG 1 cut(s) 26
BsuRI GGCC 5 cut(s) 333, 411, 970, 999, 1184
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 2 cut(s) 661, 672
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 3 cut(s) 144, 472, 867
CaiI CAGNNNCTG 1 cut(s) 356
CciI TCATGA 1 cut(s) 1197
CfoI GCGC 3 cut(s) 88, 295, 450
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 3 cut(s) 269, 456, 518
CseI GACGC 1 cut(s) 106
Csp6I GTAC 2 cut(s) 8, 547
CviAII CATG 8 cut(s) 106, 200, 496, 583, 677, 737, 767, 1198
CviQI GTAC 2 cut(s) 8, 547
DdeI CTNAG 5 cut(s) 26, 317, 444, 807, 861
DpnI GATC 6 cut(s) 225, 376, 513, 720, 958, 1193
DpnII GATC 6 cut(s) 223, 374, 511, 718, 956, 1191
DraI TTTAAA 1 cut(s) 793
DraIII CACNNNGTG 1 cut(s) 312
EaeI YGGCCR 1 cut(s) 997
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Ecl136II GAGCTC 1 cut(s) 811
Eco130I CCWWGG 3 cut(s) 190, 262, 840
Eco24I GRGCYC 2 cut(s) 244, 813
Eco47I GGWCC 3 cut(s) 269, 456, 518
Eco53kI GAGCTC 1 cut(s) 811
Eco57I CTGAAG 1 cut(s) 877
Eco81I CCTNAGG 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 553
EcoICRI GAGCTC 1 cut(s) 811
EcoRII CCWGG 3 cut(s) 217, 514, 744
EcoT14I CCWWGG 3 cut(s) 190, 262, 840
EcoT38I GRGCYC 2 cut(s) 244, 813
ErhI CCWWGG 3 cut(s) 190, 262, 840
FaeI CATG 8 cut(s) 109, 203, 499, 586, 680, 740, 770, 1201
FatI CATG 8 cut(s) 105, 199, 495, 582, 676, 736, 766, 1197
FauI CCCGC 1 cut(s) 91
Fnu4HI GCNGC 7 cut(s) 156, 351, 354, 604, 804, 846, 997
FriOI GRGCYC 2 cut(s) 244, 813
Fsp4HI GCNGC 7 cut(s) 156, 351, 354, 604, 804, 846, 997
FspBI CTAG 2 cut(s) 615, 917
GlaI GCGC 3 cut(s) 87, 294, 449
GluI GCNGC 7 cut(s) 156, 351, 354, 604, 804, 846, 997
HaeIII GGCC 5 cut(s) 333, 411, 970, 999, 1184
HapII CCGG 2 cut(s) 408, 880
HgaI GACGC 1 cut(s) 106
HhaI GCGC 3 cut(s) 88, 295, 450
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 8 cut(s) 109, 203, 499, 586, 680, 740, 770, 1201
Hin6I GCGC 3 cut(s) 86, 293, 448
HinP1I GCGC 3 cut(s) 86, 293, 448
HindIII AAGCTT 3 cut(s) 472, 533, 828
HinfI GANTC 6 cut(s) 429, 563, 611, 751, 1047, 1070
HpaII CCGG 2 cut(s) 408, 880
HphI GGTGA 6 cut(s) 218, 233, 634, 682, 829, 1164
Hpy188I TCNGA 2 cut(s) 935, 1059
Hpy188III TCNNGA 5 cut(s) 272, 555, 944, 952, 1198
HpyAV CCTTC 1 cut(s) 901
HpyCH4III ACNGT 3 cut(s) 456, 622, 656
HpyCH4IV ACGT 1 cut(s) 630
HpyF10VI GCNNNNNNNGC 5 cut(s) 152, 164, 239, 862, 1148
HpyF3I CTNAG 5 cut(s) 26, 317, 444, 807, 861
HpySE526I ACGT 1 cut(s) 630
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 8 cut(s) 109, 203, 499, 586, 680, 740, 770, 1201
HspAI GCGC 3 cut(s) 86, 293, 448
Kzo9I GATC 6 cut(s) 223, 374, 511, 718, 956, 1191
LmnI GCTCC 2 cut(s) 247, 405
Lsp1109I GCAGC 6 cut(s) 142, 340, 362, 615, 815, 832
MaeI CTAG 2 cut(s) 615, 917
MaeII ACGT 1 cut(s) 630
MaeIII GTNAC 3 cut(s) 206, 622, 835
MalI GATC 6 cut(s) 225, 376, 513, 720, 958, 1193
MboI GATC 6 cut(s) 223, 374, 511, 718, 956, 1191
MboII GAAGA 7 cut(s) 218, 311, 358, 554, 691, 754, 1186
MfeI CAATTG 2 cut(s) 17, 168
MflI RGATCY 1 cut(s) 1191
MhlI GDGCHC 2 cut(s) 244, 813
MluCI AATT 9 cut(s) 17, 69, 168, 481, 523, 711, 794, 798, 907
MlyI GAGTC 1 cut(s) 760
MmeI TCCRAC 1 cut(s) 599
MroXI GAANNNNTTC 1 cut(s) 562
MseI TTAA 6 cut(s) 44, 68, 176, 792, 797, 1163
MslI CAYNNNNRTG 1 cut(s) 204
MspA1I CMGCKG 2 cut(s) 353, 806
MspCI CTTAAG 1 cut(s) 43
MspI CCGG 2 cut(s) 408, 880
MspR9I CCNGG 4 cut(s) 219, 516, 746, 881
MunI CAATTG 2 cut(s) 17, 168
MvaI CCWGG 3 cut(s) 219, 516, 746
MvnI CGCG 3 cut(s) 86, 88, 530
MwoI GCNNNNNNNGC 5 cut(s) 152, 164, 239, 862, 1148
NciI CCSGG 1 cut(s) 881
NdeII GATC 6 cut(s) 223, 374, 511, 718, 956, 1191
NlaIII CATG 8 cut(s) 109, 203, 499, 586, 680, 740, 770, 1201
NlaIV GGNNCC 2 cut(s) 243, 401
NmuCI GTSAC 3 cut(s) 206, 622, 835
NspI RCATGY 1 cut(s) 680
PaeR7I CTCGAG 1 cut(s) 553
PagI TCATGA 1 cut(s) 1197
PaqCI CACCTGC 1 cut(s) 194
PdmI GAANNNNTTC 1 cut(s) 562
PfeI GAWTC 5 cut(s) 429, 563, 611, 1047, 1070
PfoI TCCNGGA 1 cut(s) 514
PkrI GCNGC 7 cut(s) 157, 352, 355, 605, 805, 847, 998
PleI GAGTC 1 cut(s) 759
PpsI GAGTC 1 cut(s) 759
Ppu21I YACGTR 1 cut(s) 631
PshBI ATTAAT 2 cut(s) 176, 797
Psp124BI GAGCTC 1 cut(s) 813
Psp6I CCWGG 3 cut(s) 217, 514, 744
PspGI CCWGG 3 cut(s) 217, 514, 744
PspN4I GGNNCC 2 cut(s) 243, 401
PspPI GGNCC 3 cut(s) 269, 456, 518
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 1 cut(s) 1191
PvuII CAGCTG 2 cut(s) 353, 806
RsaI GTAC 2 cut(s) 9, 548
RsaNI GTAC 2 cut(s) 8, 547
RseI CAYNNNNRTG 1 cut(s) 204
SacI GAGCTC 1 cut(s) 813
SaqAI TTAA 6 cut(s) 44, 68, 176, 792, 797, 1163
SatI GCNGC 7 cut(s) 156, 351, 354, 604, 804, 846, 997
Sau3AI GATC 6 cut(s) 223, 374, 511, 718, 956, 1191
Sau96I GGNCC 3 cut(s) 269, 456, 518
ScaI AGTACT 1 cut(s) 548
SchI GAGTC 1 cut(s) 760
ScrFI CCNGG 4 cut(s) 219, 516, 746, 881
SduI GDGCHC 2 cut(s) 244, 813
Sfr274I CTCGAG 1 cut(s) 553
SinI GGWCC 3 cut(s) 269, 456, 518
SlaI CTCGAG 1 cut(s) 553
SmiI ATTTAAAT 1 cut(s) 793
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 4 cut(s) 43, 553, 1106, 1186
SmoI CTYRAG 4 cut(s) 43, 553, 1106, 1186
Sse9I AATT 9 cut(s) 17, 69, 168, 481, 523, 711, 794, 798, 907
SsiI CCGC 5 cut(s) 84, 94, 267, 996, 1117
SspMI CTAG 2 cut(s) 615, 917
SstI GAGCTC 1 cut(s) 813
StyD4I CCNGG 4 cut(s) 217, 514, 744, 879
StyI CCWWGG 3 cut(s) 190, 262, 840
SwaI ATTTAAAT 1 cut(s) 793
TaaI ACNGT 3 cut(s) 456, 622, 656
TaiI ACGT 1 cut(s) 633
TaqI TCGA 7 cut(s) 373, 486, 554, 717, 754, 945, 1130
TaqII GACCGA 1 cut(s) 535
TasI AATT 9 cut(s) 17, 69, 168, 481, 523, 711, 794, 798, 907
TatI WGTACW 2 cut(s) 7, 546
TauI GCSGC 1 cut(s) 999
TfiI GAWTC 5 cut(s) 429, 563, 611, 1047, 1070
Tru1I TTAA 6 cut(s) 44, 68, 176, 792, 797, 1163
Tru9I TTAA 6 cut(s) 44, 68, 176, 792, 797, 1163
TscAI CASTG 2 cut(s) 661, 672
TseFI GTSAC 3 cut(s) 206, 622, 835
TseI GCWGC 6 cut(s) 155, 350, 353, 603, 803, 845
Tsp45I GTSAC 3 cut(s) 206, 622, 835
TspDTI ATGAA 7 cut(s) 109, 358, 753, 783, 840, 1083, 1186
TspRI CASTG 2 cut(s) 661, 672
Vha464I CTTAAG 1 cut(s) 43
VpaK11BI GGWCC 3 cut(s) 269, 456, 518
VspI ATTAAT 2 cut(s) 176, 797
XapI RAATTY 1 cut(s) 907
XceI RCATGY 1 cut(s) 680
XcmI CCANNNNNNNNNTGG 1 cut(s) 847
XhoI CTCGAG 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 562
XspI CTAG 2 cut(s) 615, 917
ZrmI AGTACT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.