Rroxscaffold_1G00070110

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000662
Physical Location & Seq
Reverse (-)
90947488 .. 90949640
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00070110.1

Sequence Viewer

Length: 2046 bp
ATGGTTTCTCCCATTCCTACCAGTGTCAAGAATATATGGGAAAAGTGGAATCTCCGGGGTTTTGTACTCCTCAGCCTCTCATTGCAAACTATTTTAATTCTTGGTGCACCCTTTCGAAAGCGATCACCTAGCTTATTTGTAACCTTGGTTATCTGGTCATCTTACTTGCTTGCTGACTGGGCAGCTAACTTTGCAATTGGCTTAATTGCAAACAGCCAGGGTGATAATCCATTTATTCCAAACCCCAACAACACCGGTCATTTTAATAGCGATCTTCTGGCATTTTGGGCACCCTTTCTTCTGTTACACCTTGGTGGTCCAGACACAATTACAGCATTTGCTCTTGAGGATAACACATTGTGGCTAAGGCACTTGTTTGGGCTTGTGTTCCAGGTTATAGCTGCGGTTTATGTCTTCATTCAATCATTTCCCAAAAACAAGCTGTGGCTGCCGACGGTTCTCCTATTTGTAGCAGGAATCATCAAGAATACTCTTCGAAAAAAAGATGCCAAGCTTCCAACTCATATAGAGTTGACAGCAGAGCGCAGCAAAGATTCCAGGACTACTACTTATGTGGTGTACCCTAAGGATATGGAACATAATATTGCACTGGTTCGCCATGCTTTTCACTTCTTTAACATCTTCAAGGGTCTTATTGTGGATCTCATTTTCAGCTTTCACGAACGATTTGAGAGCCGTGTGTTTTTCCATGACAGAACAGCAGAGGATGCTTTCAAGTTGGTTGCAATTGAGCTCAATTTCATATATGAAGCTCTATTCACCAAGGTGGTTGTTGTGCATTCTAAGAAGGGATACATTTGCCGAGCTATATCCTTCAGTGCAGTTTGCACAGCTCTTGTTTGCTTCCAAAAAAGAGAGAAACATGATTTCCATGGGTTTGATGTTGGAATTACCTATATTTTGCTTTTTTTTGGTGCCTTGGCCTTGGATTCAATAGCTGTGTTCATGCTTATATTCTCTGATTGGACTGTTGCTGCTGTGAAGAAGTCTTGCCAGGATTCTTGTCTTGCCAATATTCTCCAAAGGTACATCACTCTCAAACAATCTTGGTGGTTCGTTGCCGAAAATGCAAATCCATATTTGGAGAGGTGTAGGCAACTTCTATTTCGAAGGTGGTACGAATCTGTCTCCATATTTAACTTGATACATTATTCCCTCAGGGAATGTCCAAAACCGAGTCCCAATATTCTTGATTACTTCTTCATGGCCTGCATAAAAATCATCTACTTAATGGGTCTTAAGGATCTTCGAGATAAGATCAAATATAGGAAGAGCAAGCCACTCAAAAAAGAGCTATGGAAATTCATTTTTGATCAGTTGAAACGTAAGTCCATGCTCGCAGATGATCCTGAAACTGCCAGGAGAATAAGTTCAGCCAGAGGTGACTGGATTCTACAGGATAGTGACTGGAATAACTATACAGATTGTCGTAAGTTGCTCCCTTTTGTTGTTGATGTTGAATTTGATCAGAGCATTCTACTGTGGCACATTGCTACTGAGTTTTGTTACAATCTTGACAAGGAGAAGGAATCATACAAGGCTGTAGCTGTTAGCAGTGAAATCGAACATTGTAAGATCTTGTCTGATTACATGGTATACCTCTTAGTTATGCAGCCTACTATGATGTCTACCGTGGCAGGAATTGGACAAATAAGATTCCGCGATACGTGTGCAGAAGCCATAAAGTTCTTCAGCAGAAGGGAACTAGGACCAAAAGTCGCTCAAAAAGATGCATGTGAGAGAATTCTTGAGGTAAATACAGATGTTCAGCCGGTTGCTGTTAAAGGGGATCGAAGTAAATCCGTGTTGTTTGATGCATCTATTCTGGCGAAAGCACTGATGACTTTGGATGAAAAGAAATGGAAACTCATGAGTGAAGTGTGGGTGGAACTACTAGCATATGCTGCAGGTCATTGCAGAGCAAATGACCATGTCCAATTGCTGAGCAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTAGTGATGGCTCATTTTGGCATAGGGGGAGCAGTTCCTGATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

681

Amino Acids

78.31

Weight (kDa)

8.94

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 391 1.2e-80 Domain of unknown function (DUF4220)
DUF594 PF04578 625 - 674 2.5e-22 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1919
AccB1I GGYRCC 2 cut(s) 289, 935
AccI GTMKAC 2 cut(s) 1617, 1649
AccII CGCG 1 cut(s) 1683
AciI CCGC 2 cut(s) 404, 1681
AclWI GGATC 4 cut(s) 669, 1272, 1361, 1818
AcsI RAATTY 3 cut(s) 1322, 1481, 1764
AcuI CTGAAG 2 cut(s) 820, 1696
AdeI CACNNNGTG 1 cut(s) 360
AfaI GTAC 4 cut(s) 66, 581, 1049, 1139
AflII CTTAAG 1 cut(s) 1259
AflIII ACRYGT 1 cut(s) 1688
AgeI ACCGGT 1 cut(s) 254
AgsI TTSAA 6 cut(s) 422, 646, 736, 954, 1342, 1481
AhdI GACNNNNNGTC 1 cut(s) 1736
AjnI CCWGG 5 cut(s) 216, 390, 557, 1014, 1379
AleI CACNNNNGTG 2 cut(s) 312, 785
AloI GAACNNNNNNTCC 2 cut(s) 1375, 1407
Alw21I GWGCWC 2 cut(s) 109, 756
Alw26I GTCTC 1 cut(s) 1153
Alw44I GTGCAC 1 cut(s) 105
AlwI GGATC 4 cut(s) 669, 1272, 1361, 1818
AlwNI CAGNNNCTG 1 cut(s) 2036
AoxI GGCC 2 cut(s) 942, 1227
ApaLI GTGCAC 1 cut(s) 105
ApeKI GCWGC 7 cut(s) 182, 401, 448, 546, 995, 1633, 1925
ApoI RAATTY 3 cut(s) 1322, 1481, 1764
AsiGI ACCGGT 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 1390
AspLEI GCGC 1 cut(s) 546
AspS9I GGNCC 2 cut(s) 317, 1730
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 4 cut(s) 117, 233, 772, 1415
AsuII TTCGAA 3 cut(s) 115, 496, 1129
AvaII GGWCC 2 cut(s) 317, 1730
AxyI CCTNAGG 2 cut(s) 585, 1178
BaeGI GKGCMC 2 cut(s) 109, 292
BanI GGYRCC 2 cut(s) 289, 935
BanII GRGCYC 1 cut(s) 756
BarI GAAGNNNNNNTAC 2 cut(s) 1538, 1570
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 2 cut(s) 109, 756
BbvCI CCTCAGC 1 cut(s) 71
BbvI GCAGC 7 cut(s) 194, 388, 435, 558, 982, 1645, 1912
BccI CCATC 1 cut(s) 1999
BceAI ACGGC 1 cut(s) 681
BcgI CGANNNNNNTGC 4 cut(s) 1564, 1598, 1673, 1707
BciT130I CCWGG 5 cut(s) 218, 392, 559, 1016, 1381
BciVI GTATCC 1 cut(s) 806
BclI TGATCA 2 cut(s) 1333, 1486
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 1153
BfaI CTAG 3 cut(s) 129, 1727, 1916
BfmI CTRYAG 3 cut(s) 1415, 1563, 1926
BfrI CTTAAG 1 cut(s) 1259
BfuAI ACCTGC 1 cut(s) 1919
BfuI GTATCC 1 cut(s) 806
BglII AGATCT 1 cut(s) 1596
BisI GCNGC 7 cut(s) 183, 402, 449, 547, 996, 1634, 1926
BlpI GCTNAGC 1 cut(s) 1964
BlsI GCNGC 7 cut(s) 184, 403, 450, 548, 997, 1635, 1927
Bme1390I CCNGG 6 cut(s) 56, 218, 392, 559, 1016, 1381
Bme18I GGWCC 2 cut(s) 317, 1730
BmeRI GACNNNNNGTC 1 cut(s) 1736
BmgT120I GGNCC 2 cut(s) 317, 1730
BmiI GGNNCC 2 cut(s) 291, 937
BmrFI CCNGG 6 cut(s) 56, 218, 392, 559, 1016, 1381
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 5 cut(s) 496, 718, 1741, 1825, 1847
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 406
Bpu10I CCTNAGC 2 cut(s) 71, 365
Bpu1102I GCTNAGC 1 cut(s) 1964
Bpu14I TTCGAA 3 cut(s) 115, 496, 1129
BpuEI CTTGAG 2 cut(s) 365, 1790
BpuMI CCSGG 1 cut(s) 56
BsaAI YACGTR 1 cut(s) 1689
BsaJI CCNNGG 9 cut(s) 55, 144, 217, 310, 783, 892, 939, 945, 1653
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 4 cut(s) 1375, 1405, 1968, 1998
Bse118I RCCGGY 2 cut(s) 254, 1792
Bse1I ACTGG 5 cut(s) 21, 182, 615, 1412, 1433
Bse21I CCTNAGG 2 cut(s) 585, 1178
Bse3DI GCAATG 3 cut(s) 80, 1509, 1933
BseBI CCWGG 5 cut(s) 218, 392, 559, 1016, 1381
BseDI CCNNGG 9 cut(s) 55, 144, 217, 310, 783, 892, 939, 945, 1653
BseGI GGATG 2 cut(s) 733, 1876
BseMI GCAATG 3 cut(s) 80, 1509, 1933
BseMII CTCAG 4 cut(s) 85, 1192, 1509, 1955
BseNI ACTGG 5 cut(s) 21, 182, 615, 1412, 1433
BseRI GAGGAG 1 cut(s) 59
BseSI GKGCMC 2 cut(s) 109, 292
BseXI GCAGC 7 cut(s) 194, 388, 435, 558, 982, 1645, 1912
BsgI GTGCAG 2 cut(s) 861, 1713
Bsh1236I CGCG 1 cut(s) 1683
BshFI GGCC 2 cut(s) 944, 1229
BshNI GGYRCC 2 cut(s) 289, 935
BshTI ACCGGT 1 cut(s) 254
BsiHKAI GWGCWC 2 cut(s) 109, 756
BsiSI CCGG 3 cut(s) 55, 255, 1793
BslFI GGGAC 1 cut(s) 1185
BsmAI GTCTC 1 cut(s) 1153
BsmFI GGGAC 1 cut(s) 1185
BsmI GAATGC 2 cut(s) 799, 1494
BsnI GGCC 2 cut(s) 944, 1229
Bsp119I TTCGAA 3 cut(s) 115, 496, 1129
Bsp1286I GDGCHC 3 cut(s) 109, 292, 756
Bsp1720I GCTNAGC 1 cut(s) 1964
Bsp19I CCATGG 1 cut(s) 892
BspACI CCGC 2 cut(s) 404, 1681
BspANI GGCC 2 cut(s) 944, 1229
BspCNI CTCAG 4 cut(s) 84, 1191, 1510, 1956
BspFNI CGCG 1 cut(s) 1683
BspHI TCATGA 1 cut(s) 1890
BspLI GGNNCC 2 cut(s) 291, 937
BspMAI CTGCAG 1 cut(s) 1930
BspMI ACCTGC 1 cut(s) 1919
BspPI GGATC 4 cut(s) 669, 1272, 1361, 1818
BspQI GCTCTTC 1 cut(s) 1286
BspT104I TTCGAA 3 cut(s) 115, 496, 1129
BspT107I GGYRCC 2 cut(s) 289, 935
BspTI CTTAAG 1 cut(s) 1259
BsrDI GCAATG 3 cut(s) 80, 1509, 1933
BsrFI RCCGGY 2 cut(s) 254, 1792
BsrI ACTGG 5 cut(s) 21, 182, 615, 1412, 1433
BssAI RCCGGY 2 cut(s) 254, 1792
BssECI CCNNGG 9 cut(s) 55, 144, 217, 310, 783, 892, 939, 945, 1653
BssNAI GTATAC 1 cut(s) 1618
BssT1I CCWWGG 6 cut(s) 144, 310, 783, 892, 939, 945
Bst1107I GTATAC 1 cut(s) 1618
Bst2UI CCWGG 5 cut(s) 218, 392, 559, 1016, 1381
Bst4CI ACNGT 4 cut(s) 457, 991, 1503, 1654
Bst6I CTCTTC 2 cut(s) 498, 1286
BstAFI CTTAAG 1 cut(s) 1259
BstAPI GCANNNNNTGC 2 cut(s) 728, 1925
BstBAI YACGTR 1 cut(s) 1689
BstBI TTCGAA 3 cut(s) 115, 496, 1129
BstC8I GCNNGC 4 cut(s) 171, 1231, 1298, 1359
BstDEI CTNAG 8 cut(s) 71, 365, 585, 804, 1178, 1518, 1624, 1964
BstDSI CCRYGG 2 cut(s) 892, 1653
BstF5I GGATG 2 cut(s) 733, 1876
BstFNI CGCG 1 cut(s) 1683
BstHHI GCGC 1 cut(s) 546
BstMAI GTCTC 1 cut(s) 1153
BstMWI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 728, 1088, 1925
BstNI CCWGG 5 cut(s) 218, 392, 559, 1016, 1381
BstNSI RCATGY 1 cut(s) 1758
BstSCI CCNGG 6 cut(s) 54, 216, 390, 557, 1014, 1379
BstSFI CTRYAG 3 cut(s) 1415, 1563, 1926
BstSLI GKGCMC 2 cut(s) 109, 292
BstUI CGCG 1 cut(s) 1683
BstV1I GCAGC 7 cut(s) 194, 388, 435, 558, 982, 1645, 1912
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 3 cut(s) 661, 1264, 1596
BstYI RGATCY 3 cut(s) 661, 1264, 1596
BstZ17I GTATAC 1 cut(s) 1618
Bsu36I CCTNAGG 2 cut(s) 585, 1178
BsuI GTATCC 1 cut(s) 806
BsuRI GGCC 2 cut(s) 944, 1229
BtgI CCRYGG 2 cut(s) 892, 1653
BtsCI GGATG 2 cut(s) 733, 1876
BtsI GCAGTG 1 cut(s) 1582
BtsIMutI CAGTG 5 cut(s) 28, 608, 844, 1582, 1856
BveI ACCTGC 1 cut(s) 1919
Cac8I GCNNGC 4 cut(s) 171, 1231, 1298, 1359
CaiI CAGNNNCTG 1 cut(s) 2036
CciI TCATGA 1 cut(s) 1890
CfoI GCGC 1 cut(s) 546
Cfr10I RCCGGY 2 cut(s) 254, 1792
Cfr13I GGNCC 2 cut(s) 317, 1730
Csp6I GTAC 4 cut(s) 65, 580, 1048, 1138
CspAI ACCGGT 1 cut(s) 254
CspCI CAANNNNNGTGG 2 cut(s) 1052, 1087
CviQI GTAC 4 cut(s) 65, 580, 1048, 1138
DdeI CTNAG 8 cut(s) 71, 365, 585, 804, 1178, 1518, 1624, 1964
DraIII CACNNNGTG 1 cut(s) 360
DriI GACNNNNNGTC 1 cut(s) 1736
Eam1104I CTCTTC 2 cut(s) 498, 1286
Eam1105I GACNNNNNGTC 1 cut(s) 1736
EarI CTCTTC 2 cut(s) 498, 1286
Ecl136II GAGCTC 1 cut(s) 754
Eco130I CCWWGG 6 cut(s) 144, 310, 783, 892, 939, 945
Eco24I GRGCYC 1 cut(s) 756
Eco47I GGWCC 2 cut(s) 317, 1730
Eco53kI GAGCTC 1 cut(s) 754
Eco57I CTGAAG 2 cut(s) 820, 1696
Eco81I CCTNAGG 2 cut(s) 585, 1178
EcoICRI GAGCTC 1 cut(s) 754
EcoRI GAATTC 1 cut(s) 1764
EcoRII CCWGG 5 cut(s) 216, 390, 557, 1014, 1379
EcoT14I CCWWGG 6 cut(s) 144, 310, 783, 892, 939, 945
EcoT22I ATGCAT 2 cut(s) 1756, 1840
EcoT38I GRGCYC 1 cut(s) 756
ErhI CCWWGG 6 cut(s) 144, 310, 783, 892, 939, 945
FaqI GGGAC 1 cut(s) 1185
FauNDI CATATG 1 cut(s) 1921
FbaI TGATCA 2 cut(s) 1333, 1486
FblI GTMKAC 2 cut(s) 1617, 1649
Fnu4HI GCNGC 7 cut(s) 183, 402, 449, 547, 996, 1634, 1926
FokI GGATG 2 cut(s) 740, 1883
FriOI GRGCYC 1 cut(s) 756
Fsp4HI GCNGC 7 cut(s) 183, 402, 449, 547, 996, 1634, 1926
FspBI CTAG 3 cut(s) 129, 1727, 1916
GlaI GCGC 1 cut(s) 545
GluI GCNGC 7 cut(s) 183, 402, 449, 547, 996, 1634, 1926
HaeIII GGCC 2 cut(s) 944, 1229
HapII CCGG 3 cut(s) 55, 255, 1793
HhaI GCGC 1 cut(s) 546
Hin6I GCGC 1 cut(s) 544
HinP1I GCGC 1 cut(s) 544
HincII GTYRAC 1 cut(s) 534
HindII GTYRAC 1 cut(s) 534
HindIII AAGCTT 1 cut(s) 512
HpaII CCGG 3 cut(s) 55, 255, 1793
HphI GGTGA 4 cut(s) 117, 233, 772, 1415
Hpy166II GTNNAC 5 cut(s) 107, 534, 580, 1618, 1650
Hpy188I TCNGA 3 cut(s) 982, 1491, 1606
Hpy8I GTNNAC 5 cut(s) 107, 534, 580, 1618, 1650
Hpy99I CGWCG 1 cut(s) 457
HpyAV CCTTC 5 cut(s) 802, 844, 1125, 1540, 1713
HpyCH4III ACNGT 4 cut(s) 457, 991, 1503, 1654
HpyCH4IV ACGT 2 cut(s) 1345, 1688
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 728, 1088, 1925
HpyF3I CTNAG 8 cut(s) 71, 365, 585, 804, 1178, 1518, 1624, 1964
HpySE526I ACGT 2 cut(s) 1345, 1688
HspAI GCGC 1 cut(s) 544
Ksp22I TGATCA 2 cut(s) 1333, 1486
LguI GCTCTTC 1 cut(s) 1286
LmnI GCTCC 2 cut(s) 1464, 2027
Lsp1109I GCAGC 7 cut(s) 194, 388, 435, 558, 982, 1645, 1912
LweI GCATC 5 cut(s) 496, 718, 1741, 1825, 1847
MaeI CTAG 3 cut(s) 129, 1727, 1916
MaeII ACGT 2 cut(s) 1345, 1688
MaeIII GTNAC 6 cut(s) 139, 303, 1403, 1424, 1526, 1984
MfeI CAATTG 3 cut(s) 195, 747, 1958
MflI RGATCY 3 cut(s) 661, 1264, 1596
MhlI GDGCHC 3 cut(s) 109, 292, 756
MlyI GAGTC 1 cut(s) 1207
MmeI TCCRAC 2 cut(s) 542, 886
MnlI CCTC 9 cut(s) 80, 86, 340, 718, 1101, 1187, 1394, 1631, 1765
Mph1103I ATGCAT 2 cut(s) 1756, 1840
MroXI GAANNNNTTC 1 cut(s) 1390
MseI TTAA 8 cut(s) 95, 203, 264, 636, 1158, 1250, 1260, 1803
MslI CAYNNNNRTG 2 cut(s) 312, 785
MspCI CTTAAG 1 cut(s) 1259
MspI CCGG 3 cut(s) 55, 255, 1793
MspR9I CCNGG 6 cut(s) 56, 218, 392, 559, 1016, 1381
MunI CAATTG 3 cut(s) 195, 747, 1958
Mva1269I GAATGC 2 cut(s) 799, 1494
MvaI CCWGG 5 cut(s) 218, 392, 559, 1016, 1381
MvnI CGCG 1 cut(s) 1683
MwoI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 728, 1088, 1925
NciI CCSGG 1 cut(s) 56
NcoI CCATGG 1 cut(s) 892
NdeI CATATG 1 cut(s) 1921
NlaIV GGNNCC 2 cut(s) 291, 937
NmeAIII GCCGAG 1 cut(s) 848
NmuCI GTSAC 3 cut(s) 1403, 1424, 1984
NsiI ATGCAT 2 cut(s) 1756, 1840
NspI RCATGY 1 cut(s) 1758
NspV TTCGAA 3 cut(s) 115, 496, 1129
OliI CACNNNNGTG 2 cut(s) 312, 785
PagI TCATGA 1 cut(s) 1890
PciSI GCTCTTC 1 cut(s) 1286
PctI GAATGC 2 cut(s) 799, 1494
PdmI GAANNNNTTC 1 cut(s) 1390
PfeI GAWTC 9 cut(s) 49, 477, 554, 950, 1019, 1142, 1411, 1550, 1677
PflFI GACNNNGTC 1 cut(s) 1952
PfoI TCCNGGA 1 cut(s) 557
PinAI ACCGGT 1 cut(s) 254
PkrI GCNGC 7 cut(s) 184, 403, 450, 548, 997, 1635, 1927
PleI GAGTC 1 cut(s) 1206
PpsI GAGTC 1 cut(s) 1206
Ppu21I YACGTR 1 cut(s) 1689
Psp124BI GAGCTC 1 cut(s) 756
Psp6I CCWGG 5 cut(s) 216, 390, 557, 1014, 1379
PspGI CCWGG 5 cut(s) 216, 390, 557, 1014, 1379
PspN4I GGNNCC 2 cut(s) 291, 937
PspPI GGNCC 2 cut(s) 317, 1730
PstI CTGCAG 1 cut(s) 1930
PstNI CAGNNNCTG 1 cut(s) 2036
PsuI RGATCY 3 cut(s) 661, 1264, 1596
PsyI GACNNNGTC 1 cut(s) 1952
RsaI GTAC 4 cut(s) 66, 581, 1049, 1139
RsaNI GTAC 4 cut(s) 65, 580, 1048, 1138
RseI CAYNNNNRTG 2 cut(s) 312, 785
SacI GAGCTC 1 cut(s) 756
SapI GCTCTTC 1 cut(s) 1286
SaqAI TTAA 8 cut(s) 95, 203, 264, 636, 1158, 1250, 1260, 1803
SatI GCNGC 7 cut(s) 183, 402, 449, 547, 996, 1634, 1926
Sau96I GGNCC 2 cut(s) 317, 1730
SchI GAGTC 1 cut(s) 1207
ScrFI CCNGG 6 cut(s) 56, 218, 392, 559, 1016, 1381
SduI GDGCHC 3 cut(s) 109, 292, 756
SfaNI GCATC 5 cut(s) 496, 718, 1741, 1825, 1847
SfcI CTRYAG 3 cut(s) 1415, 1563, 1926
SfuI TTCGAA 3 cut(s) 115, 496, 1129
SinI GGWCC 2 cut(s) 317, 1730
SmiMI CAYNNNNRTG 2 cut(s) 312, 785
SmlI CTYRAG 3 cut(s) 344, 1259, 1769
SmoI CTYRAG 3 cut(s) 344, 1259, 1769
SsiI CCGC 2 cut(s) 404, 1681
SspI AATATT 3 cut(s) 604, 1036, 1207
SspMI CTAG 3 cut(s) 129, 1727, 1916
SstI GAGCTC 1 cut(s) 756
StyD4I CCNGG 6 cut(s) 54, 216, 390, 557, 1014, 1379
StyI CCWWGG 6 cut(s) 144, 310, 783, 892, 939, 945
TaaI ACNGT 4 cut(s) 457, 991, 1503, 1654
TaiI ACGT 2 cut(s) 1348, 1691
TaqI TCGA 6 cut(s) 115, 496, 1129, 1270, 1584, 1813
TatI WGTACW 1 cut(s) 64
TfiI GAWTC 9 cut(s) 49, 477, 554, 950, 1019, 1142, 1411, 1550, 1677
Tru1I TTAA 8 cut(s) 95, 203, 264, 636, 1158, 1250, 1260, 1803
Tru9I TTAA 8 cut(s) 95, 203, 264, 636, 1158, 1250, 1260, 1803
TscAI CASTG 5 cut(s) 28, 615, 844, 1582, 1863
TseFI GTSAC 3 cut(s) 1403, 1424, 1984
TseI GCWGC 7 cut(s) 182, 401, 448, 546, 995, 1633, 1925
Tsp45I GTSAC 3 cut(s) 1403, 1424, 1984
TspDTI ATGAA 7 cut(s) 406, 751, 783, 955, 1213, 1315, 1887
TspGWI ACGGA 1 cut(s) 1813
TspRI CASTG 5 cut(s) 28, 615, 844, 1582, 1863
Tth111I GACNNNGTC 1 cut(s) 1952
Vha464I CTTAAG 1 cut(s) 1259
VneI GTGCAC 1 cut(s) 105
VpaK11BI GGWCC 2 cut(s) 317, 1730
XapI RAATTY 3 cut(s) 1322, 1481, 1764
XceI RCATGY 1 cut(s) 1758
XmiI GTMKAC 2 cut(s) 1617, 1649
XmnI GAANNNNTTC 1 cut(s) 1390
XspI CTAG 3 cut(s) 129, 1727, 1916
Zsp2I ATGCAT 2 cut(s) 1756, 1840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.