RchiOBHm_Chr5g0005741

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000662
Physical Location & Seq
Forward (+)
3641242 .. 3644470
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28693

Sequence Viewer

Length: 2190 bp
ATGGTTTCTCCCATTCCTACCAGTGTCAAGAATATATGGGAAAAGTGGAATCTCCGGGGTTTTGTACTCCTCAGCCTCTCATTGCAAACTATTTTAATTCTTGGTGCACCCTTTCGAAAGCGATCACCTAGCTTATTTGTAACCTTGGTTATCTGGTCATCTTACTTGCTTGCTGACTGGGCAGCTAACTTTGCAATTGGCTTAATTGCAAACAGCCAGGGTGATAATCCATTTATTCCAAACCCCAACAACACCGGTCATTTTAATAGCGATCTTCTGGCATTTTGGGCGCCCTTTCTTCTGTTACACCTTGGTGGTCCAGACACAATTACAGCATTTGCTCTTGAGGATAACACATTGTGGCTGAGGCATTTGTTTGGGCTTGTGTTCCAGGTTATAGCTGCGGTTTATGTCTTCATTCAATCATTTCCCAAAAACAAGCTCTGGCTGCCGACGGTTCTCCTATTTCTAGCAGGAATCATCAAGTACGCCGAGCGCACACGTGCTCTGTATCTTGCAAGCTTGGATAATTTTAAGGAGTCCATGATTAAGAAACCTGATCCTGGTCCCAATTATGCAAAGCTGATGGAGGAATACTCTTCGAAAAAAGACGCCAAGCTTCCAACTCATATAGAGTTGACAGCAGAGCGCAGCAAAGATTCCAGGACTACTACTTATGTGGTGGACCGTGGAGATATGAAACATAATATTGCACTGGTTCGCCATGCTTTTCACTTCTTTAACATCTTCAAGGGTCTTATTGTGGATCTCATTTTCAGCTTTCATGAACGATTTGAGAGCCGTGTGTTTTTCCATGACAGAACAGCAGAGGATGCTTTCAAGTTGGTTGCAATTGAGCTCAATTTCATATATGAAGCTCTATTCACCAAGGTGGTTGTTGTGCATTCTAAGAAGGGATACATTTGCCGAGCTATATCCTTCAGTGCAGTTTGCACAGCTCTTGTTTGCTTCCAAAAAATAGAGAAACATGATTTCCATGGGTTTGATGTTGGAATTACCTATATTTTGCTTTTTGGTGCCTTGGCCTTGGATTCAATAGCTGTGTTCATGCTTATATTCTCTGATTGGACTGTTGCTGCTGTGAAGAAGTCTCGCCAGGATTCTTGTCTTGCCAATATTCTCAAAAGGTACATCACTCTCAAGCAATCTTGGTGGTTCGTTGCCGAAAATGCAAATCCATATTTGGAGAGGTGTAGGCAACTTCTATTTCGAAGGTGGTACGAATCTGTCTCCATATTCAACTTGATACATTATTCCCTCAAGGAATGTCCAAAATCGAGTCCTAATATTTTTGATTACTTCTTCATGGCCTACATAGAAATCATCTATTTACTGGGTCTTAAGGATCTTCGAGATAAGATCAAATATAGGAAGAACAAGCCACTCAAGAAAGAGCTATGGGAATTCATTTTTGCTCAGTTGAAAGGTAAGTCCATGCTCGCAGATGATCCTGAAACTGCCAGGAGAATAAGTTCGGCCAGAGGTGACTGGATTCTACAGGATAGTGACTGGAATAACTCTACAGATTGTCGTAAATTGCTCCCTTATGTTGTTGATGTTGAATATGATCAGAGCATTCTACTGTGGCACATTGCTACCGAGTTTTGTTATAATGAAATTAAACTCGAGTTTAAAGAGGATCAAACAAACAAGGCTGTTCTCACTAAAATCGAACATTGTAAGACCTTGTCTGAGTACATGGTATACCTCTTAGTTATGCAGCCTACTATGATGTCTACTGTAGCAGGAATTGGACAAATAAGATTCCGCGATACGTGTGCAGAAGCCAAAAAGTTCTTCAGCAGAAGGGAACTAGGACCAGATGTGGATCAAAAAGAGGCATGTGAGAGAATTCTCGAGGTAAATACAGATGTTAAGCCGGTTGCTGTCAAAGGGGATCGAAGTAAATCCGTGTTGTTTGACGCATGTATTCTGGCGAAAGAACTGAAGAAGATGGGCACAGAGAAATGGGATCTCATGAGTCAAGTGTGGGTGGAACTACTAGCATATGCTGCAGGTCACTGCAGAGCAAATGACCATGTCCAATTGCTGAGCAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTAGTGATGGCTCATTTTGGCATAGGGGAGCAGTTCCTGATAAATGAGGGGCATGCAAGAGCAAAACTCATTGTGGGAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

729

Amino Acids

83.9

Weight (kDa)

8.81

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 52 - 425 4.7e-104 Domain of unknown function (DUF4220)
DUF594 PF04578 660 - 710 2.6e-21 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1632
Acc36I ACCTGC 1 cut(s) 2027
AccB1I GGYRCC 2 cut(s) 289, 1037
AccI GTMKAC 2 cut(s) 1725, 1757
AccII CGCG 1 cut(s) 1791
AciI CCGC 2 cut(s) 404, 1789
AclWI GGATC 8 cut(s) 554, 774, 1374, 1463, 1668, 1857, 1926, 2001
AcoI YGGCCR 1 cut(s) 1497
AcsI RAATTY 2 cut(s) 1424, 1872
AcuI CTGAAG 3 cut(s) 925, 1804, 1988
AcvI CACGTG 1 cut(s) 503
AcyI GRCGYC 2 cut(s) 290, 612
AdeI CACNNNGTG 1 cut(s) 360
AfaI GTAC 5 cut(s) 66, 488, 1151, 1241, 1718
AfiI CCNNNNNNNGG 1 cut(s) 563
AflII CTTAAG 1 cut(s) 1361
AflIII ACRYGT 2 cut(s) 500, 1796
AgeI ACCGGT 1 cut(s) 254
AgsI TTSAA 7 cut(s) 422, 751, 841, 1056, 1261, 1444, 1583
AjnI CCWGG 6 cut(s) 216, 390, 562, 662, 1116, 1481
AjuI GAANNNNNNNTTGG 2 cut(s) 1802, 1834
AleI CACNNNNGTG 2 cut(s) 312, 890
AloI GAACNNNNNNTCC 2 cut(s) 1477, 1509
Alw21I GWGCWC 3 cut(s) 109, 508, 861
Alw26I GTCTC 2 cut(s) 1116, 1255
Alw44I GTGCAC 1 cut(s) 105
AlwI GGATC 8 cut(s) 554, 774, 1374, 1463, 1668, 1857, 1926, 2001
AlwNI CAGNNNCTG 1 cut(s) 2143
Ama87I CYCGRG 2 cut(s) 1646, 1877
AoxI GGCC 3 cut(s) 1044, 1329, 1497
ApaLI GTGCAC 1 cut(s) 105
ApeKI GCWGC 7 cut(s) 182, 401, 448, 651, 1097, 1741, 2033
ApoI RAATTY 2 cut(s) 1424, 1872
AsiGI ACCGGT 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 1492
AspLEI GCGC 3 cut(s) 292, 498, 651
AspS9I GGNCC 4 cut(s) 317, 566, 685, 1838
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 4 cut(s) 117, 233, 877, 1517
AsuII TTCGAA 3 cut(s) 115, 602, 1231
AvaI CYCGRG 2 cut(s) 1646, 1877
AvaII GGWCC 4 cut(s) 317, 566, 685, 1838
BaeGI GKGCMC 2 cut(s) 109, 1982
BanI GGYRCC 2 cut(s) 289, 1037
BanII GRGCYC 1 cut(s) 861
BbrPI CACGTG 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 3 cut(s) 109, 508, 861
BbvCI CCTCAGC 2 cut(s) 71, 365
BbvI GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1753, 2020
BccI CCATC 3 cut(s) 580, 1969, 2107
BceAI ACGGC 1 cut(s) 786
BcgI CGANNNNNNTGC 2 cut(s) 1781, 1815
BciT130I CCWGG 6 cut(s) 218, 392, 564, 664, 1118, 1483
BciVI GTATCC 1 cut(s) 911
BclI TGATCA 1 cut(s) 1588
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 2 cut(s) 1116, 1255
BfaI CTAG 4 cut(s) 129, 470, 1835, 2024
BfmI CTRYAG 5 cut(s) 1517, 1542, 1761, 2034, 2044
BfoI RGCGCY 1 cut(s) 293
BfrI CTTAAG 1 cut(s) 1361
BfuAI ACCTGC 1 cut(s) 2027
BfuI GTATCC 1 cut(s) 911
BisI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1742, 2034
BlpI GCTNAGC 1 cut(s) 2072
BlsI GCNGC 7 cut(s) 184, 403, 450, 653, 1099, 1743, 2035
Bme1390I CCNGG 7 cut(s) 56, 218, 392, 564, 664, 1118, 1483
Bme18I GGWCC 4 cut(s) 317, 566, 685, 1838
BmeT110I CYCGRG 2 cut(s) 1646, 1877
BmgT120I GGNCC 4 cut(s) 317, 566, 685, 1838
BmiI GGNNCC 3 cut(s) 291, 568, 1039
BmrFI CCNGG 7 cut(s) 56, 218, 392, 564, 664, 1118, 1483
BmrI ACTGGG 2 cut(s) 187, 1364
BmsI GCATC 1 cut(s) 823
BmuI ACTGGG 2 cut(s) 187, 1364
BpiI GAAGAC 1 cut(s) 406
BplI GAGNNNNNCTC 4 cut(s) 581, 613, 2157, 2189
Bpu10I CCTNAGC 2 cut(s) 71, 365
Bpu1102I GCTNAGC 1 cut(s) 2072
Bpu14I TTCGAA 3 cut(s) 115, 602, 1231
BpuEI CTTGAG 4 cut(s) 365, 1145, 1265, 1391
BpuMI CCSGG 1 cut(s) 56
BsaAI YACGTR 2 cut(s) 503, 1797
BsaBI GATNNNNATC 1 cut(s) 1848
BsaHI GRCGYC 2 cut(s) 290, 612
BsaJI CCNNGG 9 cut(s) 55, 144, 217, 310, 688, 888, 997, 1041, 1047
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 4 cut(s) 1477, 1507, 2076, 2106
Bsc4I CCNNNNNNNGG 1 cut(s) 563
Bse118I RCCGGY 2 cut(s) 254, 1900
Bse1I ACTGG 6 cut(s) 21, 182, 720, 1359, 1514, 1535
Bse3DI GCAATG 2 cut(s) 80, 1611
Bse8I GATNNNNATC 1 cut(s) 1848
BseBI CCWGG 6 cut(s) 218, 392, 564, 664, 1118, 1483
BseDI CCNNGG 9 cut(s) 55, 144, 217, 310, 688, 888, 997, 1041, 1047
BseGI GGATG 1 cut(s) 838
BseJI GATNNNNATC 1 cut(s) 1848
BseLI CCNNNNNNNGG 1 cut(s) 563
BseMI GCAATG 2 cut(s) 80, 1611
BseMII CTCAG 5 cut(s) 85, 356, 1451, 1704, 2063
BseNI ACTGG 6 cut(s) 21, 182, 720, 1359, 1514, 1535
BseRI GAGGAG 1 cut(s) 59
BseSI GKGCMC 2 cut(s) 109, 1982
BseXI GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1753, 2020
BsgI GTGCAG 2 cut(s) 966, 1821
Bsh1236I CGCG 1 cut(s) 1791
BshFI GGCC 3 cut(s) 1046, 1331, 1499
BshNI GGYRCC 2 cut(s) 289, 1037
BshTI ACCGGT 1 cut(s) 254
BsiHKAI GWGCWC 3 cut(s) 109, 508, 861
BsiHKCI CYCGRG 2 cut(s) 1646, 1877
BsiSI CCGG 3 cut(s) 55, 255, 1901
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 1 cut(s) 563
BsmAI GTCTC 2 cut(s) 1116, 1255
BsmFI GGGAC 1 cut(s) 552
BsmI GAATGC 2 cut(s) 904, 1596
BsnI GGCC 3 cut(s) 1046, 1331, 1499
BsoBI CYCGRG 2 cut(s) 1646, 1877
Bsp119I TTCGAA 3 cut(s) 115, 602, 1231
Bsp1286I GDGCHC 4 cut(s) 109, 508, 861, 1982
Bsp1720I GCTNAGC 1 cut(s) 2072
Bsp19I CCATGG 1 cut(s) 997
BspACI CCGC 2 cut(s) 404, 1789
BspANI GGCC 3 cut(s) 1046, 1331, 1499
BspCNI CTCAG 5 cut(s) 84, 357, 1450, 1705, 2064
BspFNI CGCG 1 cut(s) 1791
BspHI TCATGA 2 cut(s) 784, 1998
BspLI GGNNCC 3 cut(s) 291, 568, 1039
BspMAI CTGCAG 2 cut(s) 2038, 2048
BspMI ACCTGC 1 cut(s) 2027
BspPI GGATC 8 cut(s) 554, 774, 1374, 1463, 1668, 1857, 1926, 2001
BspT104I TTCGAA 3 cut(s) 115, 602, 1231
BspT107I GGYRCC 2 cut(s) 289, 1037
BspTI CTTAAG 1 cut(s) 1361
BsrDI GCAATG 2 cut(s) 80, 1611
BsrFI RCCGGY 2 cut(s) 254, 1900
BsrI ACTGG 6 cut(s) 21, 182, 720, 1359, 1514, 1535
BssAI RCCGGY 2 cut(s) 254, 1900
BssECI CCNNGG 9 cut(s) 55, 144, 217, 310, 688, 888, 997, 1041, 1047
BssNAI GTATAC 1 cut(s) 1726
BssNI GRCGYC 2 cut(s) 290, 612
BssT1I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
Bst1107I GTATAC 1 cut(s) 1726
Bst2UI CCWGG 6 cut(s) 218, 392, 564, 664, 1118, 1483
Bst4CI ACNGT 5 cut(s) 457, 689, 1093, 1605, 1762
Bst6I CTCTTC 1 cut(s) 604
BstACI GRCGYC 2 cut(s) 290, 612
BstAFI CTTAAG 1 cut(s) 1361
BstAPI GCANNNNNTGC 2 cut(s) 833, 2033
BstBAI YACGTR 2 cut(s) 503, 1797
BstBI TTCGAA 3 cut(s) 115, 602, 1231
BstC8I GCNNGC 4 cut(s) 171, 520, 1461, 2160
BstDEI CTNAG 7 cut(s) 71, 365, 909, 1437, 1713, 1732, 2072
BstDSI CCRYGG 2 cut(s) 688, 997
BstF5I GGATG 1 cut(s) 838
BstFNI CGCG 1 cut(s) 1791
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 3 cut(s) 292, 498, 651
BstMAI GTCTC 2 cut(s) 1116, 1255
BstMWI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 2033
BstNI CCWGG 6 cut(s) 218, 392, 564, 664, 1118, 1483
BstNSI RCATGY 3 cut(s) 1866, 1950, 2162
BstSCI CCNGG 7 cut(s) 54, 216, 390, 562, 662, 1116, 1481
BstSFI CTRYAG 5 cut(s) 1517, 1542, 1761, 2034, 2044
BstSLI GKGCMC 2 cut(s) 109, 1982
BstUI CGCG 1 cut(s) 1791
BstV1I GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1753, 2020
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 3 cut(s) 766, 1366, 1993
BstYI RGATCY 3 cut(s) 766, 1366, 1993
BstZ17I GTATAC 1 cut(s) 1726
BsuI GTATCC 1 cut(s) 911
BsuRI GGCC 3 cut(s) 1046, 1331, 1499
BtgI CCRYGG 2 cut(s) 688, 997
BtsCI GGATG 1 cut(s) 838
BtsI GCAGTG 1 cut(s) 2041
BtsIMutI CAGTG 4 cut(s) 28, 713, 949, 2041
BveI ACCTGC 1 cut(s) 2027
Cac8I GCNNGC 4 cut(s) 171, 520, 1461, 2160
CaiI CAGNNNCTG 1 cut(s) 2143
CciI TCATGA 2 cut(s) 784, 1998
CfoI GCGC 3 cut(s) 292, 498, 651
Cfr10I RCCGGY 2 cut(s) 254, 1900
Cfr13I GGNCC 4 cut(s) 317, 566, 685, 1838
CseI GACGC 2 cut(s) 620, 1952
Csp6I GTAC 5 cut(s) 65, 487, 1150, 1240, 1717
CspAI ACCGGT 1 cut(s) 254
CspCI CAANNNNNGTGG 2 cut(s) 1154, 1189
CviQI GTAC 5 cut(s) 65, 487, 1150, 1240, 1717
DdeI CTNAG 7 cut(s) 71, 365, 909, 1437, 1713, 1732, 2072
DinI GGCGCC 1 cut(s) 291
DraI TTTAAA 1 cut(s) 1654
DraIII CACNNNGTG 1 cut(s) 360
EaeI YGGCCR 1 cut(s) 1497
Eam1104I CTCTTC 1 cut(s) 604
EarI CTCTTC 1 cut(s) 604
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
Eco24I GRGCYC 1 cut(s) 861
Eco47I GGWCC 4 cut(s) 317, 566, 685, 1838
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 3 cut(s) 925, 1804, 1988
Eco72I CACGTG 1 cut(s) 503
Eco88I CYCGRG 2 cut(s) 1646, 1877
EcoICRI GAGCTC 1 cut(s) 859
EcoRI GAATTC 2 cut(s) 1424, 1872
EcoRII CCWGG 6 cut(s) 216, 390, 562, 662, 1116, 1481
EcoT14I CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
EcoT38I GRGCYC 1 cut(s) 861
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
FaqI GGGAC 1 cut(s) 552
FauNDI CATATG 1 cut(s) 2029
FbaI TGATCA 1 cut(s) 1588
FblI GTMKAC 2 cut(s) 1725, 1757
Fnu4HI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1742, 2034
FokI GGATG 1 cut(s) 845
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1742, 2034
FspBI CTAG 4 cut(s) 129, 470, 1835, 2024
GlaI GCGC 3 cut(s) 291, 497, 650
GluI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1742, 2034
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 3 cut(s) 1046, 1331, 1499
HapII CCGG 3 cut(s) 55, 255, 1901
HgaI GACGC 2 cut(s) 620, 1952
HhaI GCGC 3 cut(s) 292, 498, 651
Hin1I GRCGYC 2 cut(s) 290, 612
Hin6I GCGC 3 cut(s) 290, 496, 649
HinP1I GCGC 3 cut(s) 290, 496, 649
HincII GTYRAC 1 cut(s) 639
HindII GTYRAC 1 cut(s) 639
HindIII AAGCTT 2 cut(s) 520, 617
HpaII CCGG 3 cut(s) 55, 255, 1901
HphI GGTGA 4 cut(s) 117, 233, 877, 1517
Hpy166II GTNNAC 5 cut(s) 107, 639, 685, 1726, 1758
Hpy188I TCNGA 3 cut(s) 1084, 1593, 1714
Hpy8I GTNNAC 5 cut(s) 107, 639, 685, 1726, 1758
Hpy99I CGWCG 1 cut(s) 457
HpyAV CCTTC 4 cut(s) 907, 949, 1227, 1821
HpyCH4III ACNGT 5 cut(s) 457, 689, 1093, 1605, 1762
HpyCH4IV ACGT 2 cut(s) 502, 1796
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 2033
HpyF3I CTNAG 7 cut(s) 71, 365, 909, 1437, 1713, 1732, 2072
HpySE526I ACGT 2 cut(s) 502, 1796
Hsp92I GRCGYC 2 cut(s) 290, 612
HspAI GCGC 3 cut(s) 290, 496, 649
KasI GGCGCC 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 1588
LmnI GCTCC 2 cut(s) 1566, 2134
Lsp1109I GCAGC 7 cut(s) 194, 388, 435, 663, 1084, 1753, 2020
LweI GCATC 1 cut(s) 823
MaeI CTAG 4 cut(s) 129, 470, 1835, 2024
MaeII ACGT 2 cut(s) 502, 1796
MaeIII GTNAC 6 cut(s) 139, 303, 1505, 1526, 2039, 2092
MfeI CAATTG 3 cut(s) 195, 852, 2066
MflI RGATCY 3 cut(s) 766, 1366, 1993
MhlI GDGCHC 4 cut(s) 109, 508, 861, 1982
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 3 cut(s) 548, 1309, 2011
MmeI TCCRAC 2 cut(s) 647, 991
MroXI GAANNNNTTC 1 cut(s) 1492
MslI CAYNNNNRTG 2 cut(s) 312, 890
MspCI CTTAAG 1 cut(s) 1361
MspI CCGG 3 cut(s) 55, 255, 1901
MspR9I CCNGG 7 cut(s) 56, 218, 392, 564, 664, 1118, 1483
MunI CAATTG 3 cut(s) 195, 852, 2066
Mva1269I GAATGC 2 cut(s) 904, 1596
MvaI CCWGG 6 cut(s) 218, 392, 564, 664, 1118, 1483
MvnI CGCG 1 cut(s) 1791
MwoI GCNNNNNNNGC 7 cut(s) 179, 191, 287, 448, 833, 1190, 2033
NarI GGCGCC 1 cut(s) 290
NciI CCSGG 1 cut(s) 56
NcoI CCATGG 1 cut(s) 997
NdeI CATATG 1 cut(s) 2029
NlaIV GGNNCC 3 cut(s) 291, 568, 1039
NmeAIII GCCGAG 2 cut(s) 517, 953
NmuCI GTSAC 4 cut(s) 1505, 1526, 2039, 2092
NspI RCATGY 3 cut(s) 1866, 1950, 2162
NspV TTCGAA 3 cut(s) 115, 602, 1231
OliI CACNNNNGTG 2 cut(s) 312, 890
PaeI GCATGC 1 cut(s) 2162
PaeR7I CTCGAG 2 cut(s) 1646, 1877
PagI TCATGA 2 cut(s) 784, 1998
PctI GAATGC 2 cut(s) 904, 1596
PdmI GAANNNNTTC 1 cut(s) 1492
PfeI GAWTC 8 cut(s) 49, 477, 659, 1052, 1121, 1244, 1513, 1785
PflFI GACNNNGTC 2 cut(s) 1708, 2060
PfoI TCCNGGA 1 cut(s) 662
PinAI ACCGGT 1 cut(s) 254
PkrI GCNGC 7 cut(s) 184, 403, 450, 653, 1099, 1743, 2035
PleI GAGTC 3 cut(s) 547, 1308, 2010
PluTI GGCGCC 1 cut(s) 293
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 3 cut(s) 547, 1308, 2010
Ppu21I YACGTR 2 cut(s) 503, 1797
PsiI TTATAA 1 cut(s) 1632
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 6 cut(s) 216, 390, 562, 662, 1116, 1481
PspCI CACGTG 1 cut(s) 503
PspGI CCWGG 6 cut(s) 216, 390, 562, 662, 1116, 1481
PspN4I GGNNCC 3 cut(s) 291, 568, 1039
PspPI GGNCC 4 cut(s) 317, 566, 685, 1838
PspXI VCTCGAGB 1 cut(s) 1646
PstI CTGCAG 2 cut(s) 2038, 2048
PstNI CAGNNNCTG 1 cut(s) 2143
PsuI RGATCY 3 cut(s) 766, 1366, 1993
PsyI GACNNNGTC 2 cut(s) 1708, 2060
RsaI GTAC 5 cut(s) 66, 488, 1151, 1241, 1718
RsaNI GTAC 5 cut(s) 65, 487, 1150, 1240, 1717
RseI CAYNNNNRTG 2 cut(s) 312, 890
SacI GAGCTC 1 cut(s) 861
SatI GCNGC 7 cut(s) 183, 402, 449, 652, 1098, 1742, 2034
Sau96I GGNCC 4 cut(s) 317, 566, 685, 1838
SchI GAGTC 3 cut(s) 548, 1309, 2011
ScrFI CCNGG 7 cut(s) 56, 218, 392, 564, 664, 1118, 1483
SduI GDGCHC 4 cut(s) 109, 508, 861, 1982
SfaNI GCATC 1 cut(s) 823
SfcI CTRYAG 5 cut(s) 1517, 1542, 1761, 2034, 2044
SfoI GGCGCC 1 cut(s) 291
Sfr274I CTCGAG 2 cut(s) 1646, 1877
SfuI TTCGAA 3 cut(s) 115, 602, 1231
SinI GGWCC 4 cut(s) 317, 566, 685, 1838
SlaI CTCGAG 2 cut(s) 1646, 1877
SmiMI CAYNNNNRTG 2 cut(s) 312, 890
SmlI CTYRAG 7 cut(s) 344, 1160, 1280, 1361, 1406, 1646, 1877
SmoI CTYRAG 7 cut(s) 344, 1160, 1280, 1361, 1406, 1646, 1877
SphI GCATGC 1 cut(s) 2162
SsiI CCGC 2 cut(s) 404, 1789
SspDI GGCGCC 1 cut(s) 289
SspI AATATT 3 cut(s) 709, 1138, 1309
SspMI CTAG 4 cut(s) 129, 470, 1835, 2024
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 7 cut(s) 54, 216, 390, 562, 662, 1116, 1481
StyI CCWWGG 6 cut(s) 144, 310, 888, 997, 1041, 1047
TaaI ACNGT 5 cut(s) 457, 689, 1093, 1605, 1762
TaiI ACGT 2 cut(s) 505, 1799
TaqI TCGA 9 cut(s) 115, 602, 1231, 1298, 1372, 1647, 1692, 1878, 1921
TatI WGTACW 2 cut(s) 64, 1716
TfiI GAWTC 8 cut(s) 49, 477, 659, 1052, 1121, 1244, 1513, 1785
TscAI CASTG 4 cut(s) 28, 720, 949, 2048
TseFI GTSAC 4 cut(s) 1505, 1526, 2039, 2092
TseI GCWGC 7 cut(s) 182, 401, 448, 651, 1097, 1741, 2033
Tsp45I GTSAC 4 cut(s) 1505, 1526, 2039, 2092
TspGWI ACGGA 1 cut(s) 1921
TspRI CASTG 4 cut(s) 28, 720, 949, 2048
Tth111I GACNNNGTC 2 cut(s) 1708, 2060
Vha464I CTTAAG 1 cut(s) 1361
VneI GTGCAC 1 cut(s) 105
VpaK11BI GGWCC 4 cut(s) 317, 566, 685, 1838
XapI RAATTY 2 cut(s) 1424, 1872
XceI RCATGY 3 cut(s) 1866, 1950, 2162
XhoI CTCGAG 2 cut(s) 1646, 1877
XmiI GTMKAC 2 cut(s) 1725, 1757
XmnI GAANNNNTTC 1 cut(s) 1492
XspI CTAG 4 cut(s) 129, 470, 1835, 2024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.