Rh2BG250300

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000917
Physical Location & Seq
Forward (+)
25649471 .. 25651951
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG250300.1

Sequence Viewer

Length: 1596 bp
ATGGTCGTACCCAGTTTTGAGGAAATGGCAAATTCGGGGGTGGCGTTGAATTCCGAGGTGGAGTTGGCTGATCCACCTGGGGAAAGGTCACAAGATCAGCGGAAACTGAGCGCGGAGGCAATTTTCGATAAAAGTGAGGCCATGGCCAACATAAGGAGGAGTTATGGAGATCTTCGGGGCACGGAATTGAAGGTCTGTCTACTTTCTTTCTCAATCTTCCCTGAGGGTTCTAAGATAAGAAAGAGGCCTCTAATTTACTGGTGGATCGGCGAGGGCTTCATCACGGCTACCAAAGAGAAGACAGCAGAGGAGATGGGAGAAGACATCTTTGCAAAACTCTTGAGAAAGGACTTGATTCAACCAGCTACAGGTGAGTCCAACTGTGAATTACTTGTCAAAAATTGTTGTGTTCACCCTTGGATTCGTCGTATGTTGATCTCCTTGGCTAGAGAGGCTAAGCTGTTCGACTTCGATCCCAAATGGCCAATGATGCCTTCTGATTCCAAATGGCCAATGTTGCCATCTGGTTCTATATCGACCTGTAGGCGTTCATGTTTAGTTTCTAATGAGAAAAAGCCAAATCCTCAGGATAAGCCAATTCCTGAGGAAAACCCAAATCCTCAGGATAAGCCAAATTCTCGTGATGTTGATCAATGGAAACAAAATCTATTGACAGTTTTCAATGTAAACGTTCAATATCTTGATTTGAAACAAGAATGGCTGGACAAGTTGAAGAGGGTTGAAGTGCTTCACTTTGGACGCTGGCAGAGCGACCCCAAATATCACATTGAAGTGGATCAGAAAACATTTGATGGTTCACAAGCTGTATCGTTTCTAAATGGTTTGGGAGCTCAGAAGCACTTGAAGTATCTTAGCCTCAGAGGAATATCAAGAATTAGCGAACTCCCTGCTTCTATTCGTAGTCTTATCAGCCTTGAAATTCTCGATCTGAGAGCATGCCATGACTTGGAGAGGTTGCCTTCAGACATCTCATCATTGAAGAAGCTCACGCATTTGGATGTATCAGAGTGTTACTTACTAGAAGGCATGCCCAAAGGGCTTCACAAACTTTCTTCCCTGCAAGTACTTAAAGGTTTTCTGGTAGGATCACAGGAAAAAACTCCCACCAAACTTGGTGAACTTGCGACATTGAAGAAGCTAAGGAGGCTCAGCATACACATTGGGAATGGGGCTGCAATTCAAGCAGTGGAGTTCAAAAAGTTGAAAGATATGTCATCTCTTCGCTTCCTGAAGGTATCATGGGGAGTGGTTTCTAAAAATTTGACAAAGGAGATTGATGCTGAGTCCTTGAAACTCTTGTTTCCAGAGAATTTAGAAAAATTGGATCTTCAAGGCTTTCCCCTAGCACATGCACCAGAAGGGTTGAAGCCTAGCAAACTGAAAAATTTAAAGAAGCTCTATATAAGGGGTGGGGAACTTGAGAGCTTGGATCATGGGGAAAATGAGCCATGGAAAGTGGAAATCTTGCGTCTCGAGTACTTGAAGAAGTTCATAAACTCAGAAGACGAAGTGAAGAAAAATTTTCCTCATCGGAAGTACTGGACAGTGAAGAACCTCAGTAAAGCTGAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

531

Amino Acids

60.51

Weight (kDa)

8.95

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 72 - 142 3.7e-14 Disease resistance protein Winged helix domain
LRR_R13L1-DRL21 PF25019 235 - 344 8.8e-06 R13L1/DRL21 LRRs
LRR_14 PF23598 279 - 481 2.2e-27 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 967
AccI GTMKAC 1 cut(s) 199
AccII CGCG 1 cut(s) 113
AciI CCGC 2 cut(s) 100, 113
AclI AACGTT 1 cut(s) 690
AclWI GGATC 7 cut(s) 65, 272, 467, 804, 1114, 1353, 1458
AcoI YGGCCR 3 cut(s) 144, 482, 509
AcsI RAATTY 8 cut(s) 31, 49, 634, 939, 1279, 1330, 1405, 1540
AcuI CTGAAG 2 cut(s) 966, 1271
AfaI GTAC 4 cut(s) 9, 1086, 1499, 1559
AfiI CCNNNNNNNGG 3 cut(s) 153, 368, 967
AjnI CCWGG 1 cut(s) 76
AjuI GAANNNNNNNTTGG 2 cut(s) 478, 510
AluBI AGCT 9 cut(s) 365, 460, 824, 851, 1006, 1159, 1417, 1446, 1586
AluI AGCT 9 cut(s) 365, 460, 824, 851, 1006, 1159, 1417, 1446, 1586
Alw21I GWGCWC 1 cut(s) 853
Alw26I GTCTC 1 cut(s) 1496
AlwI GGATC 7 cut(s) 65, 272, 467, 804, 1114, 1353, 1458
Ama87I CYCGRG 1 cut(s) 1493
AoxI GGCC 5 cut(s) 138, 144, 245, 482, 509
ApeKI GCWGC 1 cut(s) 1193
ApoI RAATTY 8 cut(s) 31, 49, 634, 939, 1279, 1330, 1405, 1540
Asp700I GAANNNNTTC 2 cut(s) 747, 1508
AspLEI GCGC 1 cut(s) 113
AsuHPI GGTGA 3 cut(s) 383, 404, 1148
AvaI CYCGRG 1 cut(s) 1493
AxyI CCTNAGG 4 cut(s) 222, 585, 603, 621
BaeGI GKGCMC 1 cut(s) 182
BalI TGGCCA 3 cut(s) 146, 484, 511
BanII GRGCYC 1 cut(s) 853
BauI CACGAG 1 cut(s) 639
BbsI GAAGAC 3 cut(s) 305, 327, 1530
Bbv12I GWGCWC 1 cut(s) 853
BbvI GCAGC 1 cut(s) 1180
BccI CCATC 3 cut(s) 307, 529, 806
BceAI ACGGC 1 cut(s) 300
BcgI CGANNNNNNTGC 2 cut(s) 890, 924
BciT130I CCWGG 1 cut(s) 78
BclI TGATCA 1 cut(s) 649
BcoDI GTCTC 1 cut(s) 1496
BfaI CTAG 5 cut(s) 447, 1040, 1364, 1392, 1594
BfmI CTRYAG 2 cut(s) 366, 541
BglI GCCNNNNNGGC 1 cut(s) 1057
BglII AGATCT 1 cut(s) 169
BisI GCNGC 1 cut(s) 1194
BlpI GCTNAGC 2 cut(s) 456, 1169
BlsI GCNGC 1 cut(s) 1195
BmcAI AGTACT 3 cut(s) 1086, 1499, 1559
Bme1390I CCNGG 1 cut(s) 78
BmeT110I CYCGRG 1 cut(s) 1493
BmrFI CCNGG 1 cut(s) 78
BmrI ACTGGG 1 cut(s) 6
BmsI GCATC 2 cut(s) 480, 1288
BmuI ACTGGG 1 cut(s) 6
BpiI GAAGAC 3 cut(s) 305, 327, 1530
Bpu10I CCTNAGC 1 cut(s) 1160
Bpu1102I GCTNAGC 2 cut(s) 456, 1169
BpuEI CTTGAG 2 cut(s) 361, 1460
BsaBI GATNNNNATC 1 cut(s) 648
BsaJI CCNNGG 6 cut(s) 54, 77, 141, 416, 441, 1469
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 368, 967
Bse1I ACTGG 3 cut(s) 12, 263, 1565
Bse21I CCTNAGG 4 cut(s) 222, 585, 603, 621
Bse8I GATNNNNATC 1 cut(s) 648
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 6 cut(s) 54, 77, 141, 416, 441, 1469
BseGI GGATG 1 cut(s) 1024
BseJI GATNNNNATC 1 cut(s) 648
BseLI CCNNNNNNNGG 3 cut(s) 153, 368, 967
BseNI ACTGG 3 cut(s) 12, 263, 1565
BseRI GAGGAG 2 cut(s) 172, 323
BseSI GKGCMC 1 cut(s) 182
BseXI GCAGC 1 cut(s) 1180
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 5 cut(s) 140, 146, 247, 484, 511
BsiHKAI GWGCWC 1 cut(s) 853
BsiHKCI CYCGRG 1 cut(s) 1493
BslI CCNNNNNNNGG 3 cut(s) 153, 368, 967
BsmAI GTCTC 1 cut(s) 1496
BsmBI CGTCTC 1 cut(s) 1496
BsnI GGCC 5 cut(s) 140, 146, 247, 484, 511
BsoBI CYCGRG 1 cut(s) 1493
Bsp1286I GDGCHC 2 cut(s) 182, 853
Bsp1720I GCTNAGC 2 cut(s) 456, 1169
Bsp19I CCATGG 2 cut(s) 141, 1469
BspACI CCGC 2 cut(s) 100, 113
BspANI GGCC 5 cut(s) 140, 146, 247, 484, 511
BspFNI CGCG 1 cut(s) 113
BspPI GGATC 7 cut(s) 65, 272, 467, 804, 1114, 1353, 1458
BsrI ACTGG 3 cut(s) 12, 263, 1565
BssECI CCNNGG 6 cut(s) 54, 77, 141, 416, 441, 1469
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 4 cut(s) 141, 416, 441, 1469
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 3 cut(s) 383, 676, 1567
Bst6I CTCTTC 2 cut(s) 728, 1245
BstC8I GCNNGC 3 cut(s) 764, 958, 1049
BstDSI CCRYGG 2 cut(s) 141, 1469
BstF5I GGATG 1 cut(s) 1024
BstFNI CGCG 1 cut(s) 113
BstHHI GCGC 1 cut(s) 113
BstMAI GTCTC 1 cut(s) 1496
BstMWI GCNNNNNNNGC 7 cut(s) 452, 490, 517, 768, 1057, 1165, 1202
BstNI CCWGG 1 cut(s) 78
BstNSI RCATGY 3 cut(s) 960, 1051, 1373
BstSCI CCNGG 1 cut(s) 76
BstSFI CTRYAG 2 cut(s) 366, 541
BstSLI GKGCMC 1 cut(s) 182
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 1180
BstV2I GAAGAC 3 cut(s) 305, 327, 1530
BstX2I RGATCY 2 cut(s) 169, 1345
BstYI RGATCY 2 cut(s) 169, 1345
Bsu36I CCTNAGG 4 cut(s) 222, 585, 603, 621
BsuRI GGCC 5 cut(s) 140, 146, 247, 484, 511
BtgI CCRYGG 2 cut(s) 141, 1469
BtsCI GGATG 1 cut(s) 1024
BtsI GCAGTG 1 cut(s) 1212
BtsIMutI CAGTG 2 cut(s) 1212, 1572
Cac8I GCNNGC 3 cut(s) 764, 958, 1049
CfoI GCGC 1 cut(s) 113
CseI GACGC 2 cut(s) 768, 1478
Csp6I GTAC 4 cut(s) 8, 1085, 1498, 1558
CspCI CAANNNNNGTGG 2 cut(s) 1114, 1149
CviAII CATG 9 cut(s) 142, 552, 957, 962, 1048, 1260, 1370, 1454, 1470
CviQI GTAC 4 cut(s) 8, 1085, 1498, 1558
DraI TTTAAA 1 cut(s) 1410
EaeI YGGCCR 3 cut(s) 144, 482, 509
Eam1104I CTCTTC 2 cut(s) 728, 1245
EarI CTCTTC 2 cut(s) 728, 1245
Ecl136II GAGCTC 1 cut(s) 851
Eco130I CCWWGG 4 cut(s) 141, 416, 441, 1469
Eco147I AGGCCT 1 cut(s) 247
Eco24I GRGCYC 1 cut(s) 853
Eco53kI GAGCTC 1 cut(s) 851
Eco57I CTGAAG 2 cut(s) 966, 1271
Eco81I CCTNAGG 4 cut(s) 222, 585, 603, 621
Eco88I CYCGRG 1 cut(s) 1493
EcoICRI GAGCTC 1 cut(s) 851
EcoRI GAATTC 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 76
EcoT14I CCWWGG 4 cut(s) 141, 416, 441, 1469
EcoT38I GRGCYC 1 cut(s) 853
ErhI CCWWGG 4 cut(s) 141, 416, 441, 1469
Esp3I CGTCTC 1 cut(s) 1496
FaeI CATG 9 cut(s) 145, 555, 960, 965, 1051, 1263, 1373, 1457, 1473
FatI CATG 9 cut(s) 141, 551, 956, 961, 1047, 1259, 1369, 1453, 1469
FbaI TGATCA 1 cut(s) 649
FblI GTMKAC 1 cut(s) 199
Fnu4HI GCNGC 1 cut(s) 1194
FokI GGATG 1 cut(s) 1031
FriOI GRGCYC 1 cut(s) 853
Fsp4HI GCNGC 1 cut(s) 1194
FspBI CTAG 5 cut(s) 447, 1040, 1364, 1392, 1594
GlaI GCGC 1 cut(s) 112
GluI GCNGC 1 cut(s) 1194
HaeIII GGCC 5 cut(s) 140, 146, 247, 484, 511
HgaI GACGC 2 cut(s) 768, 1478
HhaI GCGC 1 cut(s) 113
Hin1II CATG 9 cut(s) 145, 555, 960, 965, 1051, 1263, 1373, 1457, 1473
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HinfI GANTC 5 cut(s) 355, 374, 421, 500, 1304
HphI GGTGA 3 cut(s) 383, 404, 1148
Hpy166II GTNNAC 5 cut(s) 200, 412, 688, 818, 1139
Hpy8I GTNNAC 5 cut(s) 200, 412, 688, 818, 1139
Hpy99I CGWCG 1 cut(s) 429
HpyAV CCTTC 7 cut(s) 184, 504, 990, 1037, 1246, 1373, 1583
HpyCH4III ACNGT 3 cut(s) 383, 676, 1567
HpyCH4IV ACGT 1 cut(s) 690
HpyCH4V TGCA 4 cut(s) 332, 1081, 1196, 1373
HpyF10VI GCNNNNNNNGC 7 cut(s) 452, 490, 517, 768, 1057, 1165, 1202
HpySE526I ACGT 1 cut(s) 690
Hsp92II CATG 9 cut(s) 145, 555, 960, 965, 1051, 1263, 1373, 1457, 1473
HspAI GCGC 1 cut(s) 111
Ksp22I TGATCA 1 cut(s) 649
LmnI GCTCC 1 cut(s) 848
Lsp1109I GCAGC 1 cut(s) 1180
LweI GCATC 2 cut(s) 480, 1288
MaeI CTAG 5 cut(s) 447, 1040, 1364, 1392, 1594
MaeII ACGT 1 cut(s) 690
MaeIII GTNAC 2 cut(s) 87, 1031
MflI RGATCY 2 cut(s) 169, 1345
MhlI GDGCHC 2 cut(s) 182, 853
MlsI TGGCCA 3 cut(s) 146, 484, 511
MluNI TGGCCA 3 cut(s) 146, 484, 511
MlyI GAGTC 2 cut(s) 383, 1313
MmeI TCCRAC 1 cut(s) 402
Mox20I TGGCCA 3 cut(s) 146, 484, 511
MroXI GAANNNNTTC 2 cut(s) 747, 1508
MscI TGGCCA 3 cut(s) 146, 484, 511
MseI TTAA 2 cut(s) 1089, 1409
MslI CAYNNNNRTG 2 cut(s) 791, 1017
Msp20I TGGCCA 3 cut(s) 146, 484, 511
MspA1I CMGCKG 1 cut(s) 100
MspR9I CCNGG 1 cut(s) 78
MvaI CCWGG 1 cut(s) 78
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 7 cut(s) 452, 490, 517, 768, 1057, 1165, 1202
NcoI CCATGG 2 cut(s) 141, 1469
NlaIII CATG 9 cut(s) 145, 555, 960, 965, 1051, 1263, 1373, 1457, 1473
NmuCI GTSAC 1 cut(s) 87
NspI RCATGY 3 cut(s) 960, 1051, 1373
PaeI GCATGC 2 cut(s) 960, 1051
PaeR7I CTCGAG 1 cut(s) 1493
PceI AGGCCT 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PdmI GAANNNNTTC 2 cut(s) 747, 1508
PfeI GAWTC 3 cut(s) 355, 421, 500
PflMI CCANNNNNTGG 1 cut(s) 967
PkrI GCNGC 1 cut(s) 1195
PleI GAGTC 2 cut(s) 382, 1312
PpsI GAGTC 2 cut(s) 382, 1312
Psp124BI GAGCTC 1 cut(s) 853
Psp1406I AACGTT 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PsuI RGATCY 2 cut(s) 169, 1345
RsaI GTAC 4 cut(s) 9, 1086, 1499, 1559
RsaNI GTAC 4 cut(s) 8, 1085, 1498, 1558
RseI CAYNNNNRTG 2 cut(s) 791, 1017
SacI GAGCTC 1 cut(s) 853
SaqAI TTAA 2 cut(s) 1089, 1409
SatI GCNGC 1 cut(s) 1194
ScaI AGTACT 3 cut(s) 1086, 1499, 1559
SchI GAGTC 2 cut(s) 383, 1313
ScrFI CCNGG 1 cut(s) 78
SduI GDGCHC 2 cut(s) 182, 853
SfaNI GCATC 2 cut(s) 480, 1288
SfcI CTRYAG 2 cut(s) 366, 541
Sfr274I CTCGAG 1 cut(s) 1493
SlaI CTCGAG 1 cut(s) 1493
SmiMI CAYNNNNRTG 2 cut(s) 791, 1017
SmlI CTYRAG 3 cut(s) 340, 1439, 1493
SmoI CTYRAG 3 cut(s) 340, 1439, 1493
SphI GCATGC 2 cut(s) 960, 1051
SseBI AGGCCT 1 cut(s) 247
SsiI CCGC 2 cut(s) 100, 113
SspMI CTAG 5 cut(s) 447, 1040, 1364, 1392, 1594
SstI GAGCTC 1 cut(s) 853
StuI AGGCCT 1 cut(s) 247
StyD4I CCNGG 1 cut(s) 76
StyI CCWWGG 4 cut(s) 141, 416, 441, 1469
TaaI ACNGT 3 cut(s) 383, 676, 1567
TaiI ACGT 1 cut(s) 693
TaqI TCGA 6 cut(s) 126, 465, 471, 536, 945, 1494
TatI WGTACW 3 cut(s) 1084, 1497, 1557
TfiI GAWTC 3 cut(s) 355, 421, 500
Tru1I TTAA 2 cut(s) 1089, 1409
Tru9I TTAA 2 cut(s) 1089, 1409
TscAI CASTG 2 cut(s) 1212, 1572
TseFI GTSAC 1 cut(s) 87
TseI GCWGC 1 cut(s) 1193
Tsp45I GTSAC 1 cut(s) 87
TspDTI ATGAA 3 cut(s) 268, 540, 1501
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 2 cut(s) 1212, 1572
Van91I CCANNNNNTGG 1 cut(s) 967
XapI RAATTY 8 cut(s) 31, 49, 634, 939, 1279, 1330, 1405, 1540
XceI RCATGY 3 cut(s) 960, 1051, 1373
XhoI CTCGAG 1 cut(s) 1493
XmiI GTMKAC 1 cut(s) 199
XmnI GAANNNNTTC 2 cut(s) 747, 1508
XspI CTAG 5 cut(s) 447, 1040, 1364, 1392, 1594
ZrmI AGTACT 3 cut(s) 1086, 1499, 1559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.