Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 56, 97 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 164 |
| AgsI | TTSAA | 1 cut(s) | 20 |
| AluBI | AGCT | 1 cut(s) | 83 |
| AluI | AGCT | 1 cut(s) | 83 |
| AoxI | GGCC | 1 cut(s) | 119 |
| AspS9I | GGNCC | 2 cut(s) | 119, 155 |
| AsuHPI | GGTGA | 1 cut(s) | 161 |
| AvaII | GGWCC | 1 cut(s) | 155 |
| BauI | CACGAG | 1 cut(s) | 84 |
| BfaI | CTAG | 1 cut(s) | 39 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 127 |
| BisI | GCNGC | 1 cut(s) | 57 |
| BlsI | GCNGC | 1 cut(s) | 58 |
| Bme18I | GGWCC | 1 cut(s) | 155 |
| BmgT120I | GGNCC | 2 cut(s) | 119, 155 |
| BmrI | ACTGGG | 1 cut(s) | 102 |
| BmuI | ACTGGG | 1 cut(s) | 102 |
| BsaJI | CCNNGG | 1 cut(s) | 145 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 164 |
| Bse1I | ACTGG | 3 cut(s) | 97, 169, 214 |
| BseDI | CCNNGG | 1 cut(s) | 145 |
| BseGI | GGATG | 1 cut(s) | 142 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 164 |
| BseNI | ACTGG | 3 cut(s) | 97, 169, 214 |
| BshFI | GGCC | 1 cut(s) | 121 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 164 |
| BsnI | GGCC | 1 cut(s) | 121 |
| Bsp19I | CCATGG | 1 cut(s) | 145 |
| BspACI | CCGC | 2 cut(s) | 56, 97 |
| BspANI | GGCC | 1 cut(s) | 121 |
| BsrI | ACTGG | 3 cut(s) | 97, 169, 214 |
| BssECI | CCNNGG | 1 cut(s) | 145 |
| BssSI | CACGAG | 1 cut(s) | 84 |
| BssT1I | CCWWGG | 1 cut(s) | 145 |
| Bst2BI | CACGAG | 1 cut(s) | 84 |
| Bst6I | CTCTTC | 1 cut(s) | 177 |
| BstDSI | CCRYGG | 1 cut(s) | 145 |
| BstF5I | GGATG | 1 cut(s) | 142 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 53, 127 |
| BsuRI | GGCC | 1 cut(s) | 121 |
| BtgI | CCRYGG | 1 cut(s) | 145 |
| BtsCI | GGATG | 1 cut(s) | 142 |
| BtsIMutI | CAGTG | 1 cut(s) | 207 |
| Cfr13I | GGNCC | 2 cut(s) | 119, 155 |
| CviAII | CATG | 3 cut(s) | 62, 133, 146 |
| CviJI | RGCY | 5 cut(s) | 47, 59, 83, 121, 130 |
| CviKI_1 | RGCY | 5 cut(s) | 47, 59, 83, 121, 130 |
| Eam1104I | CTCTTC | 1 cut(s) | 177 |
| EarI | CTCTTC | 1 cut(s) | 177 |
| Eco130I | CCWWGG | 1 cut(s) | 145 |
| Eco47I | GGWCC | 1 cut(s) | 155 |
| EcoT14I | CCWWGG | 1 cut(s) | 145 |
| ErhI | CCWWGG | 1 cut(s) | 145 |
| FaeI | CATG | 3 cut(s) | 65, 136, 149 |
| FaiI | YATR | 9 cut(s) | 33, 53, 63, 102, 134, 147, 161, 222, 227 |
| FatI | CATG | 3 cut(s) | 61, 132, 145 |
| Fnu4HI | GCNGC | 1 cut(s) | 57 |
| FokI | GGATG | 1 cut(s) | 149 |
| Fsp4HI | GCNGC | 1 cut(s) | 57 |
| FspBI | CTAG | 1 cut(s) | 39 |
| GluI | GCNGC | 1 cut(s) | 57 |
| HaeIII | GGCC | 1 cut(s) | 121 |
| Hin1II | CATG | 3 cut(s) | 65, 136, 149 |
| HinfI | GANTC | 2 cut(s) | 5, 75 |
| HphI | GGTGA | 1 cut(s) | 161 |
| Hpy166II | GTNNAC | 1 cut(s) | 204 |
| Hpy188III | TCNNGA | 1 cut(s) | 86 |
| Hpy8I | GTNNAC | 1 cut(s) | 204 |
| HpyAV | CCTTC | 1 cut(s) | 132 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 53, 127 |
| Hsp92II | CATG | 3 cut(s) | 65, 136, 149 |
| LpnPI | CCDG | 4 cut(s) | 57, 78, 150, 195 |
| MaeI | CTAG | 1 cut(s) | 39 |
| MaeIII | GTNAC | 1 cut(s) | 149 |
| MboII | GAAGA | 1 cut(s) | 194 |
| MnlI | CCTC | 1 cut(s) | 35 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 53, 127 |
| NcoI | CCATGG | 1 cut(s) | 145 |
| NlaIII | CATG | 3 cut(s) | 65, 136, 149 |
| NmuCI | GTSAC | 1 cut(s) | 149 |
| PfeI | GAWTC | 2 cut(s) | 5, 75 |
| PkrI | GCNGC | 1 cut(s) | 58 |
| PspPI | GGNCC | 2 cut(s) | 119, 155 |
| SatI | GCNGC | 1 cut(s) | 57 |
| Sau96I | GGNCC | 2 cut(s) | 119, 155 |
| SetI | ASST | 2 cut(s) | 85, 160 |
| SinI | GGWCC | 1 cut(s) | 155 |
| SsiI | CCGC | 2 cut(s) | 56, 97 |
| SspMI | CTAG | 1 cut(s) | 39 |
| StyI | CCWWGG | 1 cut(s) | 145 |
| TauI | GCSGC | 1 cut(s) | 59 |
| TfiI | GAWTC | 2 cut(s) | 5, 75 |
| TscAI | CASTG | 1 cut(s) | 214 |
| TseFI | GTSAC | 1 cut(s) | 149 |
| Tsp45I | GTSAC | 1 cut(s) | 149 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 1 cut(s) | 168 |
| TspRI | CASTG | 1 cut(s) | 214 |
| VpaK11BI | GGWCC | 1 cut(s) | 155 |
| XspI | CTAG | 1 cut(s) | 39 |