Rh2DG516600

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000917
Physical Location & Seq
Forward (+)
74002030 .. 74004239
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG516600.1

Sequence Viewer

Length: 1872 bp
ATGTCAAACTCATTGTTTTCAAGAGATCCAGACAAGTTCATGCCCAAGTTGCTGGATCATCTGAGAAAAGCCAGAGCCGTGGCCGACACAGATGACGAAGCTTTACTCGTGAGCTTCAAAAAGATGGAAGATGAGCTTGAGAACATCAAAGGCGTGCTACCCAGAGTGAAGCACTGGGAGCAAACTCTGGTGAACCAGTTCACTAACTTGGAGCGCCGCCTCGACAAGATCATCTTGGGCACTGAGACTTCCATCGAGGCCATCACCGCCACACTAAACCGCATGTTTCAAAATGTGGAGCAAATCAAACTCTCACATCCTCTAGTGCAGCTGATGCCTTCACCCGAGCGCAGCTCCGAGTCGACATGGACGCGCAGATCTCAGCCGGAGTTGAGTCAGGGAATGTCACCCGAATGGTCCGAATTGAGCCTCGAAAATTGGGTTGATAACACTCGGAGCATGGAGGATATAAAGAGAAGCTATGAACATCTTGAGAGCGTTGAGTTGAAGATGTGCTGTCTATCTCTTTCAATATTCCCTCCTGATTCTGTCATAAAGAAGAGGCCTCTGATTTACTGGTGGATTGGCGAGGGTTTCGTTACAACCACTAAAGGCAGGACAGCTGAAGAGACAGGTGAGGAAATCTTCCGGAAACTCATAATCTCGGGTTTGATTCAAGTTCAACCACATAATGCATTTAGTTCTATAGTTGTGAATAGTTGTACAGTGCATCCTTGGGTTCGTCATATGTTGGTCTCCTTGGCCAAGCACGCTAAGCTTTTCGACTTCGATTCAAGATGGCCAAGGATGCCGTCTTATAGTACAGATAGTATTTCAAATTGTAGACGTGCATGTTTAGTTTATGACAACCCAATTTCTGAGGGGTCTAATGGCCGGAAAGTGGAGGATCTGTTGACAGTGTTCAATGTGAACGAACAGTATCTTAGTTTGAAACGGGAATGGCTTAGGAAGTTGAAAAAAGTTGAGGTGATTCAGCTAGGACGGTGGCAGAACTCGGCTTCGCATCACATTGAAGTCGAAGATAAAGGGTTTTTGAAAGGTTTAGGGGCTCAGAATAAGCTCTTGAAGTATCTTTGCCTCCGAGGGATATCAGGAATTACCAAACTCCCTTCTTCTATCTTGAAGCTTCTCAACCTTGAAATTCTTGATCTGAGGGCATGCCACAGCTTAGAGACTTTGCCTCCAGATATCTCACCATTGAGGAATCTCACGCATTTCGACATATCCGAGTGTTACTTGCTAGAAGCAATGCCGAAAGGAATTGAGAAGCTGTCTTCTCTTCAAGTGCTAAAAGGCTTTGTGATTGGGGATTTGGAAGGAACTCCCTGCAGAATTGGTGATCTTGCACAGCTGAGGCATCTAAAGCGGCTCAGTATACACATGGGCAATCAGGCTGAAGTTCAAAAGGGGGAGTTCCACAAATTGGAGGAAATTGTATCTCTTCGCCACCTCAAACTTTCATGGGGAGTTGTTTCTCCAAACTTGAAAGCAAAAGTTGCCTTCCAGTCCTTGGAGTTCTCTTTTCCGCCGGATTTAGAAAAATTGGATCTTCAAGGCATCCCTCTAGCACATGTACCAGAATGGTTGAACCCTGTCAAGCTCAAGAATTTAAAAAAGCTCTTTATAAGGGGTGGGGAACTAGATAGCTTGGATCATGGAGAGACTGAGAAGTGGACTGTTGAAGTCTTGCGTCTCAAGCACTTGAATAATTTGGAGATAGGTTTGCCAAAGTTGAAGGATGAGTTTCCTCGTCTGCGCTATTTGGAGAAAATCAACTGTCATGAGATTGAAAAGGATCGATACGATGAAGACATTGTTTGGAGTGAATATGCAGAGTACACCTGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

623

Amino Acids

71.76

Weight (kDa)

6.87

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 178 - 248 3.2e-13 Disease resistance protein Winged helix domain
LRR_14 PF23598 361 - 554 2.3e-24 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1646
AccB7I CCANNNNNTGG 2 cut(s) 1444, 1531
AccI GTMKAC 3 cut(s) 362, 844, 1396
AccII CGCG 1 cut(s) 373
AccIII TCCGGA 1 cut(s) 648
AciI CCGC 5 cut(s) 217, 267, 280, 1387, 1547
AclWI GGATC 6 cut(s) 20, 63, 915, 1575, 1680, 1824
AcoI YGGCCR 4 cut(s) 81, 762, 800, 892
AcsI RAATTY 2 cut(s) 1161, 1627
AcuI CTGAAG 2 cut(s) 645, 1437
AfaI GTAC 4 cut(s) 724, 823, 1596, 1859
AfiI CCNNNNNNNGG 3 cut(s) 901, 1444, 1531
AflIII ACRYGT 1 cut(s) 1591
AjiI CACGTC 1 cut(s) 848
Alw26I GTCTC 6 cut(s) 239, 623, 760, 1187, 1676, 1718
AlwI GGATC 6 cut(s) 20, 63, 915, 1575, 1680, 1824
Ama87I CYCGRG 2 cut(s) 344, 664
Aor13HI TCCGGA 1 cut(s) 648
AoxI GGCC 6 cut(s) 81, 258, 563, 762, 800, 892
ApeKI GCWGC 2 cut(s) 328, 351
ApoI RAATTY 2 cut(s) 1161, 1627
Asp700I GAANNNNTTC 1 cut(s) 197
AspLEI GCGC 4 cut(s) 216, 351, 375, 1779
AspS9I GGNCC 1 cut(s) 417
AsuHPI GGTGA 8 cut(s) 202, 256, 333, 399, 647, 1000, 1206, 1370
AvaI CYCGRG 2 cut(s) 344, 664
AvaII GGWCC 1 cut(s) 417
BaeGI GKGCMC 1 cut(s) 242
BalI TGGCCA 2 cut(s) 764, 802
BanII GRGCYC 1 cut(s) 1072
BauI CACGAG 1 cut(s) 107
BbsI GAAGAC 2 cut(s) 1287, 1836
BbvCI CCTCAGC 1 cut(s) 1373
BbvI GCAGC 2 cut(s) 340, 363
BccI CCATC 4 cut(s) 118, 260, 269, 792
BceAI ACGGC 2 cut(s) 62, 796
BcoDI GTCTC 6 cut(s) 239, 623, 760, 1187, 1676, 1718
BfaI CTAG 5 cut(s) 323, 998, 1262, 1586, 1661
BfmI CTRYAG 2 cut(s) 705, 1348
BfoI RGCGCY 1 cut(s) 217
BglII AGATCT 1 cut(s) 377
BisI GCNGC 4 cut(s) 217, 329, 352, 1388
BlpI GCTNAGC 1 cut(s) 774
BlsI GCNGC 4 cut(s) 218, 330, 353, 1389
Bme18I GGWCC 1 cut(s) 417
BmeT110I CYCGRG 2 cut(s) 344, 664
BmgBI CACGTC 1 cut(s) 848
BmgT120I GGNCC 1 cut(s) 417
BmrI ACTGGG 1 cut(s) 184
BmsI GCATC 6 cut(s) 324, 739, 798, 1033, 1387, 1587
BmuI ACTGGG 1 cut(s) 184
BpiI GAAGAC 2 cut(s) 1287, 1836
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpmI CTGGAG 1 cut(s) 1188
Bpu10I CCTNAGC 2 cut(s) 965, 1373
Bpu1102I GCTNAGC 1 cut(s) 774
BpuEI CTTGAG 4 cut(s) 158, 512, 1607, 1700
Bsa29I ATCGAT 1 cut(s) 1819
BsaI GGTCTC 1 cut(s) 760
BsaJI CCNNGG 6 cut(s) 78, 734, 759, 803, 1102, 1530
BsaWI WCCGGW 1 cut(s) 648
BsaXI ACNNNNNCTCC 4 cut(s) 896, 926, 1186, 1216
Bsc4I CCNNNNNNNGG 3 cut(s) 901, 1444, 1531
Bse1I ACTGG 4 cut(s) 179, 196, 581, 1525
Bse3DI GCAATG 1 cut(s) 1275
BseAI TCCGGA 1 cut(s) 648
BseCI ATCGAT 1 cut(s) 1819
BseDI CCNNGG 6 cut(s) 78, 734, 759, 803, 1102, 1530
BseGI GGATG 5 cut(s) 316, 730, 813, 1578, 1765
BseLI CCNNNNNNNGG 3 cut(s) 901, 1444, 1531
BseMI GCAATG 1 cut(s) 1275
BseMII CTCAG 9 cut(s) 53, 234, 395, 870, 1085, 1163, 1364, 1405, 1677
BseNI ACTGG 4 cut(s) 179, 196, 581, 1525
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 2 cut(s) 340, 363
BsgI GTGCAG 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 373
BshFI GGCC 6 cut(s) 83, 260, 565, 764, 802, 894
BshVI ATCGAT 1 cut(s) 1819
BsiHKCI CYCGRG 2 cut(s) 344, 664
BsiSI CCGG 4 cut(s) 386, 649, 895, 1550
BslI CCNNNNNNNGG 3 cut(s) 901, 1444, 1531
BsmAI GTCTC 6 cut(s) 239, 623, 760, 1187, 1676, 1718
BsmBI CGTCTC 1 cut(s) 1718
BsnI GGCC 6 cut(s) 83, 260, 565, 764, 802, 894
Bso31I GGTCTC 1 cut(s) 760
BsoBI CYCGRG 2 cut(s) 344, 664
Bsp1286I GDGCHC 2 cut(s) 242, 1072
Bsp13I TCCGGA 1 cut(s) 648
Bsp1407I TGTACA 1 cut(s) 722
Bsp1720I GCTNAGC 1 cut(s) 774
BspACI CCGC 5 cut(s) 217, 267, 280, 1387, 1547
BspANI GGCC 6 cut(s) 83, 260, 565, 764, 802, 894
BspCNI CTCAG 9 cut(s) 54, 235, 394, 871, 1084, 1164, 1365, 1404, 1678
BspDI ATCGAT 1 cut(s) 1819
BspEI TCCGGA 1 cut(s) 648
BspFNI CGCG 1 cut(s) 373
BspHI TCATGA 1 cut(s) 1801
BspMAI CTGCAG 1 cut(s) 1352
BspPI GGATC 6 cut(s) 20, 63, 915, 1575, 1680, 1824
BspTNI GGTCTC 1 cut(s) 760
BsrDI GCAATG 1 cut(s) 1275
BsrGI TGTACA 1 cut(s) 722
BsrI ACTGG 4 cut(s) 179, 196, 581, 1525
BssECI CCNNGG 6 cut(s) 78, 734, 759, 803, 1102, 1530
BssNAI GTATAC 1 cut(s) 1397
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 4 cut(s) 734, 759, 803, 1530
Bst1107I GTATAC 1 cut(s) 1397
Bst2BI CACGAG 1 cut(s) 107
Bst4CI ACNGT 6 cut(s) 727, 919, 939, 1005, 1699, 1799
Bst6I CTCTTC 4 cut(s) 554, 621, 1305, 1467
BstAPI GCANNNNNTGC 2 cut(s) 334, 1517
BstAUI TGTACA 1 cut(s) 722
BstC8I GCNNGC 3 cut(s) 155, 771, 1180
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 5 cut(s) 316, 730, 813, 1578, 1765
BstFNI CGCG 1 cut(s) 373
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 4 cut(s) 216, 351, 375, 1779
BstMAI GTCTC 6 cut(s) 239, 623, 760, 1187, 1676, 1718
BstNSI RCATGY 4 cut(s) 286, 855, 1182, 1595
BstSFI CTRYAG 2 cut(s) 705, 1348
BstSLI GKGCMC 1 cut(s) 242
BstUI CGCG 1 cut(s) 373
BstV1I GCAGC 2 cut(s) 340, 363
BstV2I GAAGAC 2 cut(s) 1287, 1836
BstX2I RGATCY 4 cut(s) 25, 377, 907, 1567
BstXI CCANNNNNNTGG 2 cut(s) 52, 79
BstYI RGATCY 4 cut(s) 25, 377, 907, 1567
BstZ17I GTATAC 1 cut(s) 1397
Bsu15I ATCGAT 1 cut(s) 1819
BsuRI GGCC 6 cut(s) 83, 260, 565, 764, 802, 894
BsuTUI ATCGAT 1 cut(s) 1819
BtgI CCRYGG 1 cut(s) 78
BtrI CACGTC 1 cut(s) 848
BtsCI GGATG 5 cut(s) 316, 730, 813, 1578, 1765
BtsIMutI CAGTG 4 cut(s) 172, 240, 732, 924
Cac8I GCNNGC 3 cut(s) 155, 771, 1180
CciI TCATGA 1 cut(s) 1801
CfoI GCGC 4 cut(s) 216, 351, 375, 1779
Cfr13I GGNCC 1 cut(s) 417
ClaI ATCGAT 1 cut(s) 1819
CseI GACGC 2 cut(s) 379, 1700
Csp6I GTAC 4 cut(s) 723, 822, 1595, 1858
CviQI GTAC 4 cut(s) 723, 822, 1595, 1858
DraI TTTAAA 1 cut(s) 1632
EaeI YGGCCR 4 cut(s) 81, 762, 800, 892
Eam1104I CTCTTC 4 cut(s) 554, 621, 1305, 1467
EarI CTCTTC 4 cut(s) 554, 621, 1305, 1467
EciI GGCGGA 1 cut(s) 1536
Eco130I CCWWGG 4 cut(s) 734, 759, 803, 1530
Eco147I AGGCCT 1 cut(s) 565
Eco24I GRGCYC 1 cut(s) 1072
Eco31I GGTCTC 1 cut(s) 760
Eco32I GATATC 2 cut(s) 1110, 1210
Eco47I GGWCC 1 cut(s) 417
Eco57I CTGAAG 2 cut(s) 645, 1437
Eco88I CYCGRG 2 cut(s) 344, 664
EcoRV GATATC 2 cut(s) 1110, 1210
EcoT14I CCWWGG 4 cut(s) 734, 759, 803, 1530
EcoT22I ATGCAT 1 cut(s) 697
EcoT38I GRGCYC 1 cut(s) 1072
ErhI CCWWGG 4 cut(s) 734, 759, 803, 1530
Esp3I CGTCTC 1 cut(s) 1718
FalI AAGNNNNNCTT 4 cut(s) 120, 152, 218, 250
FauNDI CATATG 2 cut(s) 747, 1868
FblI GTMKAC 3 cut(s) 362, 844, 1396
Fnu4HI GCNGC 4 cut(s) 217, 329, 352, 1388
FokI GGATG 5 cut(s) 303, 717, 820, 1565, 1772
FriOI GRGCYC 1 cut(s) 1072
Fsp4HI GCNGC 4 cut(s) 217, 329, 352, 1388
FspBI CTAG 5 cut(s) 323, 998, 1262, 1586, 1661
GlaI GCGC 4 cut(s) 215, 350, 374, 1778
GluI GCNGC 4 cut(s) 217, 329, 352, 1388
GsuI CTGGAG 1 cut(s) 1188
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 6 cut(s) 83, 260, 565, 764, 802, 894
HapII CCGG 4 cut(s) 386, 649, 895, 1550
HgaI GACGC 2 cut(s) 379, 1700
HhaI GCGC 4 cut(s) 216, 351, 375, 1779
Hin6I GCGC 4 cut(s) 214, 349, 373, 1777
HinP1I GCGC 4 cut(s) 214, 349, 373, 1777
HincII GTYRAC 2 cut(s) 363, 915
HindII GTYRAC 2 cut(s) 363, 915
HindIII AAGCTT 3 cut(s) 99, 776, 1145
HinfI GANTC 7 cut(s) 359, 394, 545, 673, 791, 991, 1225
HpaII CCGG 4 cut(s) 386, 649, 895, 1550
HphI GGTGA 8 cut(s) 202, 256, 333, 399, 647, 1000, 1206, 1370
Hpy166II GTNNAC 9 cut(s) 193, 201, 363, 845, 915, 931, 1397, 1695, 1860
Hpy8I GTNNAC 9 cut(s) 193, 201, 363, 845, 915, 931, 1397, 1695, 1860
HpyAV CCTTC 5 cut(s) 348, 1140, 1331, 1531, 1750
HpyCH4III ACNGT 6 cut(s) 727, 919, 939, 1005, 1699, 1799
HpyCH4IV ACGT 1 cut(s) 847
HpyCH4V TGCA 8 cut(s) 328, 695, 730, 851, 1350, 1367, 1853, 1866
HpySE526I ACGT 1 cut(s) 847
HspAI GCGC 4 cut(s) 214, 349, 373, 1777
Kpn2I TCCGGA 1 cut(s) 648
LmnI GCTCC 5 cut(s) 178, 211, 298, 359, 456
Lsp1109I GCAGC 2 cut(s) 340, 363
LweI GCATC 6 cut(s) 324, 739, 798, 1033, 1387, 1587
MaeI CTAG 5 cut(s) 323, 998, 1262, 1586, 1661
MaeII ACGT 1 cut(s) 847
MaeIII GTNAC 3 cut(s) 405, 598, 1253
MflI RGATCY 4 cut(s) 25, 377, 907, 1567
MhlI GDGCHC 2 cut(s) 242, 1072
MlsI TGGCCA 2 cut(s) 764, 802
MluNI TGGCCA 2 cut(s) 764, 802
MlyI GAGTC 2 cut(s) 368, 403
Mox20I TGGCCA 2 cut(s) 764, 802
Mph1103I ATGCAT 1 cut(s) 697
MroI TCCGGA 1 cut(s) 648
MroXI GAANNNNTTC 1 cut(s) 197
MscI TGGCCA 2 cut(s) 764, 802
MseI TTAA 1 cut(s) 1631
MslI CAYNNNNRTG 1 cut(s) 412
Msp20I TGGCCA 2 cut(s) 764, 802
MspA1I CMGCKG 3 cut(s) 331, 623, 1372
MspI CCGG 4 cut(s) 386, 649, 895, 1550
MvnI CGCG 1 cut(s) 373
NdeI CATATG 2 cut(s) 747, 1868
NmeAIII GCCGAG 1 cut(s) 995
NmuCI GTSAC 1 cut(s) 405
NsiI ATGCAT 1 cut(s) 697
NspI RCATGY 4 cut(s) 286, 855, 1182, 1595
PaeI GCATGC 1 cut(s) 1182
PagI TCATGA 1 cut(s) 1801
PceI AGGCCT 1 cut(s) 565
PciI ACATGT 1 cut(s) 1591
PcsI WCGNNNNNNNCGW 1 cut(s) 1245
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 5 cut(s) 545, 673, 791, 991, 1225
PflMI CCANNNNNTGG 2 cut(s) 1444, 1531
PkrI GCNGC 4 cut(s) 218, 330, 353, 1389
PleI GAGTC 2 cut(s) 367, 402
PpsI GAGTC 2 cut(s) 367, 402
PscI ACATGT 1 cut(s) 1591
PsiI TTATAA 1 cut(s) 1646
PspPI GGNCC 1 cut(s) 417
PstI CTGCAG 1 cut(s) 1352
PsuI RGATCY 4 cut(s) 25, 377, 907, 1567
PvuII CAGCTG 3 cut(s) 331, 623, 1372
RsaI GTAC 4 cut(s) 724, 823, 1596, 1859
RsaNI GTAC 4 cut(s) 723, 822, 1595, 1858
RseI CAYNNNNRTG 1 cut(s) 412
SalI GTCGAC 1 cut(s) 361
SaqAI TTAA 1 cut(s) 1631
SatI GCNGC 4 cut(s) 217, 329, 352, 1388
Sau96I GGNCC 1 cut(s) 417
SchI GAGTC 2 cut(s) 368, 403
SduI GDGCHC 2 cut(s) 242, 1072
SfaNI GCATC 6 cut(s) 324, 739, 798, 1033, 1387, 1587
SfcI CTRYAG 2 cut(s) 705, 1348
SinI GGWCC 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 412
SmlI CTYRAG 4 cut(s) 137, 491, 1622, 1715
SmoI CTYRAG 4 cut(s) 137, 491, 1622, 1715
SphI GCATGC 1 cut(s) 1182
SseBI AGGCCT 1 cut(s) 565
SsiI CCGC 5 cut(s) 217, 267, 280, 1387, 1547
SspI AATATT 1 cut(s) 534
SspMI CTAG 5 cut(s) 323, 998, 1262, 1586, 1661
StuI AGGCCT 1 cut(s) 565
StyI CCWWGG 4 cut(s) 734, 759, 803, 1530
TaaI ACNGT 6 cut(s) 727, 919, 939, 1005, 1699, 1799
TaiI ACGT 1 cut(s) 850
TaqI TCGA 9 cut(s) 222, 255, 362, 432, 783, 789, 1038, 1239, 1819
TatI WGTACW 3 cut(s) 722, 821, 1857
TauI GCSGC 2 cut(s) 219, 1390
TfiI GAWTC 5 cut(s) 545, 673, 791, 991, 1225
Tru1I TTAA 1 cut(s) 1631
Tru9I TTAA 1 cut(s) 1631
TscAI CASTG 4 cut(s) 179, 247, 732, 924
TseFI GTSAC 1 cut(s) 405
TseI GCWGC 2 cut(s) 328, 351
Tsp45I GTSAC 1 cut(s) 405
TspDTI ATGAA 4 cut(s) 28, 498, 1470, 1842
TspRI CASTG 4 cut(s) 179, 247, 732, 924
Van91I CCANNNNNTGG 2 cut(s) 1444, 1531
VpaK11BI GGWCC 1 cut(s) 417
XapI RAATTY 2 cut(s) 1161, 1627
XceI RCATGY 4 cut(s) 286, 855, 1182, 1595
XmiI GTMKAC 3 cut(s) 362, 844, 1396
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 5 cut(s) 323, 998, 1262, 1586, 1661
Zsp2I ATGCAT 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.