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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 10 |
| AgsI | TTSAA | 1 cut(s) | 81 |
| Alw26I | GTCTC | 1 cut(s) | 84 |
| ApoI | RAATTY | 1 cut(s) | 10 |
| BbsI | GAAGAC | 1 cut(s) | 66 |
| BclI | TGATCA | 1 cut(s) | 3 |
| BcoDI | GTCTC | 1 cut(s) | 84 |
| BglII | AGATCT | 1 cut(s) | 31 |
| BmsI | GCATC | 1 cut(s) | 54 |
| BpiI | GAAGAC | 1 cut(s) | 66 |
| Bse3DI | GCAATG | 1 cut(s) | 53 |
| BseMI | GCAATG | 1 cut(s) | 53 |
| BseRI | GAGGAG | 2 cut(s) | 31, 39 |
| BseYI | CCCAGC | 1 cut(s) | 67 |
| BsmAI | GTCTC | 1 cut(s) | 84 |
| Bsp143I | GATC | 2 cut(s) | 3, 31 |
| BsrDI | GCAATG | 1 cut(s) | 53 |
| BssMI | GATC | 2 cut(s) | 3, 31 |
| BstKTI | GATC | 2 cut(s) | 6, 34 |
| BstMAI | GTCTC | 1 cut(s) | 84 |
| BstMBI | GATC | 2 cut(s) | 3, 31 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 64 |
| BstV2I | GAAGAC | 1 cut(s) | 66 |
| BstX2I | RGATCY | 1 cut(s) | 31 |
| BstYI | RGATCY | 1 cut(s) | 31 |
| DpnI | GATC | 2 cut(s) | 5, 33 |
| DpnII | GATC | 2 cut(s) | 3, 31 |
| EcoRI | GAATTC | 1 cut(s) | 10 |
| FbaI | TGATCA | 1 cut(s) | 3 |
| FspEI | CC | 8 cut(s) | 20, 21, 53, 54, 55, 63, 66, 95 |
| GsaI | CCCAGC | 1 cut(s) | 71 |
| Hpy188III | TCNNGA | 1 cut(s) | 7 |
| HpyCH4V | TGCA | 1 cut(s) | 98 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 64 |
| Ksp22I | TGATCA | 1 cut(s) | 3 |
| Kzo9I | GATC | 2 cut(s) | 3, 31 |
| LpnPI | CCDG | 2 cut(s) | 20, 53 |
| LweI | GCATC | 1 cut(s) | 54 |
| MalI | GATC | 2 cut(s) | 5, 33 |
| MboI | GATC | 2 cut(s) | 3, 31 |
| MboII | GAAGA | 2 cut(s) | 66, 69 |
| MflI | RGATCY | 1 cut(s) | 31 |
| MluCI | AATT | 1 cut(s) | 10 |
| MnlI | CCTC | 2 cut(s) | 9, 60 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 64 |
| NdeII | GATC | 2 cut(s) | 3, 31 |
| PspFI | CCCAGC | 1 cut(s) | 67 |
| PsuI | RGATCY | 1 cut(s) | 31 |
| Sau3AI | GATC | 2 cut(s) | 3, 31 |
| SfaNI | GCATC | 1 cut(s) | 54 |
| SgeI | CNNG | 5 cut(s) | 19, 27, 47, 80, 93 |
| Sse9I | AATT | 1 cut(s) | 10 |
| TaqI | TCGA | 1 cut(s) | 14 |
| TasI | AATT | 1 cut(s) | 10 |
| XapI | RAATTY | 1 cut(s) | 10 |