Rh2AG442100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001198
Physical Location & Seq
Reverse (-)
65330369 .. 65372556
42188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG442100.1

Sequence Viewer

Length: 789 bp
ATGATCAGACGTCTAGAGATTTTGGCAAATCCGGATAGAACATTAGGATTTTGGGAACAGTGCCCTGTCAGATTTTCTAATTGCTTCAGAGTTCAGATTCAGAGGATTACTGCTATGACTGCAAAACCCAATGTCCCAATCAATGTTTTAAGTGTAATCCAGACGAAGAAGGTTTATGATACTGGTCATCGTATAGCCCTGCAACTACCTAGAGATGTGTATGATAGGTTCCCCGATAAGGTGTTGGAGTTCTTGAATAGTCAGGCAGTGGATGCTGAAGGGGCACCAGTGGGTGTTGATACTGGCGAGGTGCAAGATTTAGGTTTTTTTCCTTTCATATTGTGGTTTTCTATTTTTGGATTAACGTCGGGGAAAGATGCAAGGGGCTTTTGGCTTAGAAGACCACAGCAGCTAACAAACATAAGGACAAGGATACTTAGAAAGACTTTTCTTCTTGTCCACATAATCAGGTGGTTTGGAGGCTCCTTTGTGCCGAGTTTTGTGACTTCTCTACTTCTCCTTTGGTTGTTTGCTAATGTAGGCCAGTTTCGCATTCATCTCATACCAAAATTCAGCGAAATTGTATATAGGTCATATTGGCATGGTGGTTCTGGTATTACTCCAATGCTTCAGGTCATTGACGCTACACTAAAAAATCTTGATGACACAACCAAGGGCCAGCGACAAGTGTCAGAAGGAGAAGAGGAAGCATGCACTTATCCTACATTTTCAAAGGAGCAAGCAAGTGCACTAGTGTTGCCCACTGCCACATTAGTCAATTCGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

29.8

Weight (kDa)

9.37

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 13
AccB1I GGYRCC 1 cut(s) 283
AccIII TCCGGA 1 cut(s) 31
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 3 cut(s) 70, 297, 614
AcyI GRCGYC 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 238
AgsI TTSAA 2 cut(s) 256, 732
AhlI ACTAGT 1 cut(s) 751
AluBI AGCT 1 cut(s) 412
AluI AGCT 1 cut(s) 412
Alw21I GWGCWC 1 cut(s) 751
Alw44I GTGCAC 1 cut(s) 747
Aor13HI TCCGGA 1 cut(s) 31
AoxI GGCC 2 cut(s) 541, 676
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 1 cut(s) 409
ApoI RAATTY 1 cut(s) 569
AspS9I GGNCC 1 cut(s) 676
BaeGI GKGCMC 3 cut(s) 65, 286, 751
BanI GGYRCC 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 1 cut(s) 751
BbvI GCAGC 1 cut(s) 421
BciVI GTATCC 1 cut(s) 426
BclI TGATCA 1 cut(s) 3
BcuI ACTAGT 1 cut(s) 751
BfaI CTAG 3 cut(s) 14, 210, 752
BfuI GTATCC 1 cut(s) 426
BisI GCNGC 1 cut(s) 410
BlsI GCNGC 1 cut(s) 411
BmgT120I GGNCC 1 cut(s) 676
BmiI GGNNCC 3 cut(s) 230, 285, 484
BmsI GCATC 2 cut(s) 262, 367
BoxI GACNNNNGTC 1 cut(s) 688
BpiI GAAGAC 1 cut(s) 406
BsaHI GRCGYC 1 cut(s) 10
BsaJI CCNNGG 1 cut(s) 672
BsaWI WCCGGW 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse1I ACTGG 4 cut(s) 187, 287, 307, 544
BseAI TCCGGA 1 cut(s) 31
BseDI CCNNGG 1 cut(s) 672
BseGI GGATG 1 cut(s) 277
BseLI CCNNNNNNNGG 1 cut(s) 238
BseNI ACTGG 4 cut(s) 187, 287, 307, 544
BseSI GKGCMC 3 cut(s) 65, 286, 751
BseXI GCAGC 1 cut(s) 421
BshFI GGCC 2 cut(s) 543, 678
BshNI GGYRCC 1 cut(s) 283
BsiHKAI GWGCWC 1 cut(s) 751
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 552
BsnI GGCC 2 cut(s) 543, 678
Bsp1286I GDGCHC 3 cut(s) 65, 286, 751
Bsp13I TCCGGA 1 cut(s) 31
Bsp143I GATC 1 cut(s) 3
BspANI GGCC 2 cut(s) 543, 678
BspEI TCCGGA 1 cut(s) 31
BspLI GGNNCC 3 cut(s) 230, 285, 484
BspT107I GGYRCC 1 cut(s) 283
BsrI ACTGG 4 cut(s) 187, 287, 307, 544
BssECI CCNNGG 1 cut(s) 672
BssMI GATC 1 cut(s) 3
BssNI GRCGYC 1 cut(s) 10
BssT1I CCWWGG 1 cut(s) 672
Bst4CI ACNGT 1 cut(s) 60
Bst6I CTCTTC 1 cut(s) 696
BstACI GRCGYC 1 cut(s) 10
BstAPI GCANNNNNTGC 1 cut(s) 272
BstC8I GCNNGC 3 cut(s) 680, 712, 741
BstDEI CTNAG 2 cut(s) 395, 437
BstF5I GGATG 1 cut(s) 277
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 4 cut(s) 119, 272, 281, 549
BstNSI RCATGY 1 cut(s) 714
BstPAI GACNNNNGTC 1 cut(s) 688
BstSLI GKGCMC 3 cut(s) 65, 286, 751
BstV1I GCAGC 1 cut(s) 421
BstV2I GAAGAC 1 cut(s) 406
BsuI GTATCC 1 cut(s) 426
BsuRI GGCC 2 cut(s) 543, 678
BtsCI GGATG 1 cut(s) 277
BtsI GCAGTG 2 cut(s) 273, 762
BtsIMutI CAGTG 4 cut(s) 65, 273, 294, 762
Cac8I GCNNGC 3 cut(s) 680, 712, 741
Cfr13I GGNCC 1 cut(s) 676
CseI GACGC 1 cut(s) 650
CviAII CATG 2 cut(s) 602, 711
CviJI RGCY 7 cut(s) 197, 387, 394, 412, 483, 543, 678
CviKI_1 RGCY 7 cut(s) 197, 387, 394, 412, 483, 543, 678
DdeI CTNAG 2 cut(s) 395, 437
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 696
EarI CTCTTC 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 672
Eco57I CTGAAG 3 cut(s) 70, 297, 614
EcoT14I CCWWGG 1 cut(s) 672
ErhI CCWWGG 1 cut(s) 672
FaeI CATG 2 cut(s) 605, 714
FaqI GGGAC 1 cut(s) 119
FatI CATG 2 cut(s) 601, 710
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 410
FokI GGATG 1 cut(s) 284
Fsp4HI GCNGC 1 cut(s) 410
FspBI CTAG 3 cut(s) 14, 210, 752
GluI GCNGC 1 cut(s) 410
HaeIII GGCC 2 cut(s) 543, 678
HapII CCGG 1 cut(s) 32
HgaI GACGC 1 cut(s) 650
Hin1I GRCGYC 1 cut(s) 10
Hin1II CATG 2 cut(s) 605, 714
HinfI GANTC 1 cut(s) 97
HpaII CCGG 1 cut(s) 32
Hpy166II GTNNAC 2 cut(s) 460, 749
Hpy188I TCNGA 6 cut(s) 8, 71, 89, 96, 102, 694
Hpy188III TCNNGA 5 cut(s) 14, 32, 160, 253, 659
Hpy8I GTNNAC 2 cut(s) 460, 749
Hpy99I CGWCG 1 cut(s) 370
HpyAV CCTTC 3 cut(s) 163, 272, 689
HpyCH4III ACNGT 1 cut(s) 60
HpyCH4IV ACGT 2 cut(s) 10, 365
HpyCH4V TGCA 6 cut(s) 122, 202, 313, 380, 714, 749
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 272, 281, 549
HpyF3I CTNAG 2 cut(s) 395, 437
HpySE526I ACGT 2 cut(s) 10, 365
Hsp92I GRCGYC 1 cut(s) 10
Hsp92II CATG 2 cut(s) 605, 714
Kpn2I TCCGGA 1 cut(s) 31
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 2 cut(s) 488, 736
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 2 cut(s) 262, 367
MaeI CTAG 3 cut(s) 14, 210, 752
MaeII ACGT 2 cut(s) 10, 365
MaeIII GTNAC 1 cut(s) 502
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 4 cut(s) 178, 411, 443, 713
MhlI GDGCHC 3 cut(s) 65, 286, 751
MluCI AATT 4 cut(s) 79, 569, 579, 778
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 4 cut(s) 96, 301, 473, 697
MroI TCCGGA 1 cut(s) 31
MseI TTAA 2 cut(s) 149, 362
MspI CCGG 1 cut(s) 32
Mva1269I GAATGC 1 cut(s) 552
MwoI GCNNNNNNNGC 4 cut(s) 119, 272, 281, 549
NdeII GATC 1 cut(s) 3
NlaIII CATG 2 cut(s) 605, 714
NlaIV GGNNCC 3 cut(s) 230, 285, 484
NmeAIII GCCGAG 1 cut(s) 519
NmuCI GTSAC 1 cut(s) 502
NspI RCATGY 1 cut(s) 714
PaeI GCATGC 1 cut(s) 714
PctI GAATGC 1 cut(s) 552
PfeI GAWTC 1 cut(s) 97
PkrI GCNGC 1 cut(s) 411
PshAI GACNNNNGTC 1 cut(s) 688
PspN4I GGNNCC 3 cut(s) 230, 285, 484
PspPI GGNCC 1 cut(s) 676
PsrI GAACNNNNNNTAC 2 cut(s) 212, 244
SaqAI TTAA 2 cut(s) 149, 362
SatI GCNGC 1 cut(s) 410
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 1 cut(s) 676
SduI GDGCHC 3 cut(s) 65, 286, 751
SfaNI GCATC 2 cut(s) 262, 367
SpeI ACTAGT 1 cut(s) 751
SphI GCATGC 1 cut(s) 714
Sse9I AATT 4 cut(s) 79, 569, 579, 778
SspMI CTAG 3 cut(s) 14, 210, 752
StyI CCWWGG 1 cut(s) 672
TaaI ACNGT 1 cut(s) 60
TaiI ACGT 2 cut(s) 13, 368
TasI AATT 4 cut(s) 79, 569, 579, 778
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 2 cut(s) 149, 362
Tru9I TTAA 2 cut(s) 149, 362
TscAI CASTG 4 cut(s) 65, 273, 294, 769
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 2 cut(s) 325, 545
TspRI CASTG 4 cut(s) 65, 273, 294, 769
VneI GTGCAC 1 cut(s) 747
XapI RAATTY 1 cut(s) 569
XbaI TCTAGA 1 cut(s) 13
XceI RCATGY 1 cut(s) 714
XspI CTAG 3 cut(s) 14, 210, 752
ZraI GACGTC 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.