Belongs to the ubiquitin-conjugating enzyme family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 3 cut(s) | 104, 712, 1172 |
| AatII | GACGTC | 1 cut(s) | 45 |
| Acc16I | TGCGCA | 1 cut(s) | 173 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 430, 1390 |
| AccBSI | CCGCTC | 1 cut(s) | 312 |
| AccI | GTMKAC | 1 cut(s) | 192 |
| AccII | CGCG | 3 cut(s) | 124, 126, 548 |
| AclI | AACGTT | 1 cut(s) | 572 |
| AclWI | GGATC | 2 cut(s) | 156, 433 |
| AcoI | YGGCCR | 2 cut(s) | 943, 1236 |
| AcsI | RAATTY | 4 cut(s) | 135, 601, 836, 1324 |
| AcvI | CACGTG | 2 cut(s) | 188, 381 |
| AcyI | GRCGYC | 4 cut(s) | 42, 1221, 1260, 1341 |
| AfaI | GTAC | 6 cut(s) | 103, 179, 344, 487, 773, 1198 |
| AfeI | AGCGCT | 1 cut(s) | 447 |
| AflIII | ACRYGT | 5 cut(s) | 185, 187, 355, 378, 546 |
| AgeI | ACCGGT | 1 cut(s) | 501 |
| AgsI | TTSAA | 4 cut(s) | 31, 558, 751, 1252 |
| AjnI | CCWGG | 2 cut(s) | 740, 940 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 11, 43, 435, 467 |
| AluBI | AGCT | 4 cut(s) | 781, 923, 971, 1066 |
| AluI | AGCT | 4 cut(s) | 781, 923, 971, 1066 |
| Alw21I | GWGCWC | 1 cut(s) | 1068 |
| Alw26I | GTCTC | 6 cut(s) | 176, 341, 692, 698, 1021, 1268 |
| AlwI | GGATC | 2 cut(s) | 156, 433 |
| Ama87I | CYCGRG | 2 cut(s) | 391, 1140 |
| Aor51HI | AGCGCT | 1 cut(s) | 447 |
| AoxI | GGCC | 8 cut(s) | 3, 388, 930, 943, 1143, 1206, 1236, 1284 |
| ApeKI | GCWGC | 2 cut(s) | 968, 1043 |
| ApoI | RAATTY | 4 cut(s) | 135, 601, 836, 1324 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 491, 523 |
| AsiGI | ACCGGT | 1 cut(s) | 501 |
| AspLEI | GCGC | 4 cut(s) | 126, 174, 448, 1043 |
| AspS9I | GGNCC | 5 cut(s) | 52, 389, 1143, 1206, 1284 |
| AsuC2I | CCSGG | 7 cut(s) | 155, 272, 392, 393, 483, 1141, 1142 |
| AsuHPI | GGTGA | 1 cut(s) | 1373 |
| AsuII | TTCGAA | 1 cut(s) | 1296 |
| AvaI | CYCGRG | 2 cut(s) | 391, 1140 |
| AvaII | GGWCC | 1 cut(s) | 52 |
| AxyI | CCTNAGG | 1 cut(s) | 200 |
| BanII | GRGCYC | 1 cut(s) | 1068 |
| BbrPI | CACGTG | 2 cut(s) | 188, 381 |
| BbsI | GAAGAC | 2 cut(s) | 708, 1097 |
| Bbv12I | GWGCWC | 1 cut(s) | 1068 |
| BbvI | GCAGC | 2 cut(s) | 980, 1030 |
| BccI | CCATC | 5 cut(s) | 425, 868, 1154, 1233, 1313 |
| BceAI | ACGGC | 3 cut(s) | 1248, 1351, 1366 |
| BciT130I | CCWGG | 2 cut(s) | 742, 942 |
| BclI | TGATCA | 1 cut(s) | 1126 |
| BcnI | CCSGG | 7 cut(s) | 155, 272, 392, 393, 483, 1141, 1142 |
| BcoDI | GTCTC | 6 cut(s) | 176, 341, 692, 698, 1021, 1268 |
| BfoI | RGCGCY | 2 cut(s) | 449, 1044 |
| BisI | GCNGC | 8 cut(s) | 326, 946, 969, 997, 1025, 1044, 1236, 1344 |
| BlpI | GCTNAGC | 1 cut(s) | 924 |
| BlsI | GCNGC | 8 cut(s) | 327, 947, 970, 998, 1026, 1045, 1237, 1345 |
| Bme1390I | CCNGG | 9 cut(s) | 155, 272, 392, 393, 483, 742, 942, 1141, 1142 |
| Bme18I | GGWCC | 1 cut(s) | 52 |
| BmeT110I | CYCGRG | 2 cut(s) | 391, 1140 |
| BmgT120I | GGNCC | 5 cut(s) | 52, 389, 1143, 1206, 1284 |
| BmiI | GGNNCC | 2 cut(s) | 54, 1285 |
| BmrFI | CCNGG | 9 cut(s) | 155, 272, 392, 393, 483, 742, 942, 1141, 1142 |
| BmrI | ACTGGG | 1 cut(s) | 498 |
| BmuI | ACTGGG | 1 cut(s) | 498 |
| BoxI | GACNNNNGTC | 1 cut(s) | 188 |
| BpiI | GAAGAC | 2 cut(s) | 708, 1097 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 924 |
| Bpu14I | TTCGAA | 1 cut(s) | 1296 |
| BpuEI | CTTGAG | 1 cut(s) | 385 |
| BpuMI | CCSGG | 7 cut(s) | 155, 272, 392, 393, 483, 1141, 1142 |
| BsaAI | YACGTR | 4 cut(s) | 169, 177, 188, 381 |
| BsaBI | GATNNNNATC | 1 cut(s) | 279 |
| BsaHI | GRCGYC | 4 cut(s) | 42, 1221, 1260, 1341 |
| BsaI | GGTCTC | 1 cut(s) | 1268 |
| BsaJI | CCNNGG | 6 cut(s) | 391, 392, 759, 1140, 1239, 1263 |
| BsaWI | WCCGGW | 2 cut(s) | 501, 826 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 755, 785 |
| Bse118I | RCCGGY | 4 cut(s) | 501, 928, 1037, 1286 |
| Bse1I | ACTGG | 3 cut(s) | 355, 493, 860 |
| Bse21I | CCTNAGG | 1 cut(s) | 200 |
| Bse8I | GATNNNNATC | 1 cut(s) | 279 |
| BseBI | CCWGG | 2 cut(s) | 742, 942 |
| BseDI | CCNNGG | 6 cut(s) | 391, 392, 759, 1140, 1239, 1263 |
| BseGI | GGATG | 3 cut(s) | 404, 417, 526 |
| BseJI | GATNNNNATC | 1 cut(s) | 279 |
| BseMII | CTCAG | 4 cut(s) | 72, 214, 644, 693 |
| BseNI | ACTGG | 3 cut(s) | 355, 493, 860 |
| BseRI | GAGGAG | 2 cut(s) | 188, 898 |
| BseX3I | CGGCCG | 1 cut(s) | 1236 |
| BseXI | GCAGC | 2 cut(s) | 980, 1030 |
| Bsh1236I | CGCG | 3 cut(s) | 124, 126, 548 |
| Bsh1285I | CGRYCG | 2 cut(s) | 502, 1239 |
| BshFI | GGCC | 8 cut(s) | 5, 390, 932, 945, 1145, 1208, 1238, 1286 |
| BshTI | ACCGGT | 1 cut(s) | 501 |
| BsiEI | CGRYCG | 2 cut(s) | 502, 1239 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1068 |
| BsiHKCI | CYCGRG | 2 cut(s) | 391, 1140 |
| BsiWI | CGTACG | 1 cut(s) | 177 |
| BslFI | GGGAC | 4 cut(s) | 413, 511, 557, 1138 |
| BsmAI | GTCTC | 6 cut(s) | 176, 341, 692, 698, 1021, 1268 |
| BsmBI | CGTCTC | 1 cut(s) | 1021 |
| BsmFI | GGGAC | 4 cut(s) | 413, 511, 557, 1138 |
| BsnI | GGCC | 8 cut(s) | 5, 390, 932, 945, 1145, 1208, 1238, 1286 |
| Bso31I | GGTCTC | 1 cut(s) | 1268 |
| BsoBI | CYCGRG | 2 cut(s) | 391, 1140 |
| Bsp119I | TTCGAA | 1 cut(s) | 1296 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1068 |
| Bsp143I | GATC | 5 cut(s) | 117, 148, 438, 904, 1126 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 924 |
| Bsp19I | CCATGG | 1 cut(s) | 759 |
| BspANI | GGCC | 8 cut(s) | 5, 390, 932, 945, 1145, 1208, 1238, 1286 |
| BspCNI | CTCAG | 4 cut(s) | 71, 213, 645, 694 |
| BspFNI | CGCG | 3 cut(s) | 124, 126, 548 |
| BspLI | GGNNCC | 2 cut(s) | 54, 1285 |
| BspPI | GGATC | 2 cut(s) | 156, 433 |
| BspQI | GCTCTTC | 1 cut(s) | 631 |
| BspT104I | TTCGAA | 1 cut(s) | 1296 |
| BspTNI | GGTCTC | 1 cut(s) | 1268 |
| BsrBI | CCGCTC | 1 cut(s) | 312 |
| BsrFI | RCCGGY | 4 cut(s) | 501, 928, 1037, 1286 |
| BsrI | ACTGG | 3 cut(s) | 355, 493, 860 |
| BssAI | RCCGGY | 4 cut(s) | 501, 928, 1037, 1286 |
| BssECI | CCNNGG | 6 cut(s) | 391, 392, 759, 1140, 1239, 1263 |
| BssMI | GATC | 5 cut(s) | 117, 148, 438, 904, 1126 |
| BssNI | GRCGYC | 4 cut(s) | 42, 1221, 1260, 1341 |
| BssT1I | CCWWGG | 2 cut(s) | 759, 1263 |
| Bst2UI | CCWGG | 2 cut(s) | 742, 942 |
| Bst4CI | ACNGT | 6 cut(s) | 24, 80, 101, 106, 539, 1100 |
| Bst6I | CTCTTC | 2 cut(s) | 254, 631 |
| BstACI | GRCGYC | 4 cut(s) | 42, 1221, 1260, 1341 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 322 |
| BstBAI | YACGTR | 4 cut(s) | 169, 177, 188, 381 |
| BstBI | TTCGAA | 1 cut(s) | 1296 |
| BstC8I | GCNNGC | 6 cut(s) | 314, 661, 930, 1039, 1288, 1312 |
| BstDEI | CTNAG | 6 cut(s) | 58, 200, 653, 702, 924, 1033 |
| BstDSI | CCRYGG | 1 cut(s) | 759 |
| BstF5I | GGATG | 3 cut(s) | 404, 417, 526 |
| BstFNI | CGCG | 3 cut(s) | 124, 126, 548 |
| BstH2I | RGCGCY | 2 cut(s) | 449, 1044 |
| BstHHI | GCGC | 4 cut(s) | 126, 174, 448, 1043 |
| BstKTI | GATC | 5 cut(s) | 120, 151, 441, 907, 1129 |
| BstMAI | GTCTC | 6 cut(s) | 176, 341, 692, 698, 1021, 1268 |
| BstMBI | GATC | 5 cut(s) | 117, 148, 438, 904, 1126 |
| BstMCI | CGRYCG | 2 cut(s) | 502, 1239 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 322, 617, 665, 690, 929, 968, 1043 |
| BstNI | CCWGG | 2 cut(s) | 742, 942 |
| BstNSI | RCATGY | 2 cut(s) | 359, 564 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 188 |
| BstSCI | CCNGG | 9 cut(s) | 153, 270, 390, 391, 481, 740, 940, 1139, 1140 |
| BstUI | CGCG | 3 cut(s) | 124, 126, 548 |
| BstV1I | GCAGC | 2 cut(s) | 980, 1030 |
| BstV2I | GAAGAC | 2 cut(s) | 708, 1097 |
| BstX2I | RGATCY | 1 cut(s) | 148 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 632 |
| BstYI | RGATCY | 1 cut(s) | 148 |
| BstZI | CGGCCG | 1 cut(s) | 1236 |
| Bsu36I | CCTNAGG | 1 cut(s) | 200 |
| BsuRI | GGCC | 8 cut(s) | 5, 390, 932, 945, 1145, 1208, 1238, 1286 |
| BtgI | CCRYGG | 1 cut(s) | 759 |
| BtgZI | GCGATG | 1 cut(s) | 1291 |
| BtsCI | GGATG | 3 cut(s) | 404, 417, 526 |
| BtsI | GCAGTG | 1 cut(s) | 615 |
| BtsIMutI | CAGTG | 3 cut(s) | 544, 615, 1096 |
| Cac8I | GCNNGC | 6 cut(s) | 314, 661, 930, 1039, 1288, 1312 |
| CfoI | GCGC | 4 cut(s) | 126, 174, 448, 1043 |
| Cfr10I | RCCGGY | 4 cut(s) | 501, 928, 1037, 1286 |
| Cfr13I | GGNCC | 5 cut(s) | 52, 389, 1143, 1206, 1284 |
| Cfr9I | CCCGGG | 2 cut(s) | 391, 1140 |
| CseI | GACGC | 4 cut(s) | 554, 1210, 1268, 1349 |
| Csp6I | GTAC | 6 cut(s) | 102, 178, 343, 486, 772, 1197 |
| CspAI | ACCGGT | 1 cut(s) | 501 |
| CviAII | CATG | 6 cut(s) | 356, 514, 561, 760, 1130, 1385 |
| CviQI | GTAC | 6 cut(s) | 102, 178, 343, 486, 772, 1197 |
| DdeI | CTNAG | 6 cut(s) | 58, 200, 653, 702, 924, 1033 |
| DpnI | GATC | 5 cut(s) | 119, 150, 440, 906, 1128 |
| DpnII | GATC | 5 cut(s) | 117, 148, 438, 904, 1126 |
| DraI | TTTAAA | 1 cut(s) | 796 |
| DrdI | GACNNNNNNGTC | 3 cut(s) | 104, 712, 1172 |
| DseDI | GACNNNNNNGTC | 3 cut(s) | 104, 712, 1172 |
| EaeI | YGGCCR | 2 cut(s) | 943, 1236 |
| EagI | CGGCCG | 1 cut(s) | 1236 |
| Eam1104I | CTCTTC | 2 cut(s) | 254, 631 |
| EarI | CTCTTC | 2 cut(s) | 254, 631 |
| EciI | GGCGGA | 3 cut(s) | 258, 1305, 1368 |
| Ecl136II | GAGCTC | 1 cut(s) | 1066 |
| EclXI | CGGCCG | 1 cut(s) | 1236 |
| Eco130I | CCWWGG | 2 cut(s) | 759, 1263 |
| Eco24I | GRGCYC | 1 cut(s) | 1068 |
| Eco31I | GGTCTC | 1 cut(s) | 1268 |
| Eco47I | GGWCC | 1 cut(s) | 52 |
| Eco47III | AGCGCT | 1 cut(s) | 447 |
| Eco52I | CGGCCG | 1 cut(s) | 1236 |
| Eco53kI | GAGCTC | 1 cut(s) | 1066 |
| Eco72I | CACGTG | 2 cut(s) | 188, 381 |
| Eco81I | CCTNAGG | 1 cut(s) | 200 |
| Eco88I | CYCGRG | 2 cut(s) | 391, 1140 |
| EcoICRI | GAGCTC | 1 cut(s) | 1066 |
| EcoRII | CCWGG | 2 cut(s) | 740, 940 |
| EcoT14I | CCWWGG | 2 cut(s) | 759, 1263 |
| EcoT38I | GRGCYC | 1 cut(s) | 1068 |
| ErhI | CCWWGG | 2 cut(s) | 759, 1263 |
| Esp3I | CGTCTC | 1 cut(s) | 1021 |
| FaeI | CATG | 6 cut(s) | 359, 517, 564, 763, 1133, 1388 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 455, 487, 700, 732, 917, 949 |
| FaqI | GGGAC | 4 cut(s) | 413, 511, 557, 1138 |
| FatI | CATG | 6 cut(s) | 355, 513, 560, 759, 1129, 1384 |
| FbaI | TGATCA | 1 cut(s) | 1126 |
| FblI | GTMKAC | 1 cut(s) | 192 |
| Fnu4HI | GCNGC | 8 cut(s) | 326, 946, 969, 997, 1025, 1044, 1236, 1344 |
| FokI | GGATG | 3 cut(s) | 391, 404, 533 |
| FriOI | GRGCYC | 1 cut(s) | 1068 |
| Fsp4HI | GCNGC | 8 cut(s) | 326, 946, 969, 997, 1025, 1044, 1236, 1344 |
| FspAI | RTGCGCAY | 1 cut(s) | 173 |
| FspI | TGCGCA | 1 cut(s) | 173 |
| GlaI | GCGC | 4 cut(s) | 125, 173, 447, 1042 |
| GluI | GCNGC | 8 cut(s) | 326, 946, 969, 997, 1025, 1044, 1236, 1344 |
| HaeII | RGCGCY | 2 cut(s) | 449, 1044 |
| HaeIII | GGCC | 8 cut(s) | 5, 390, 932, 945, 1145, 1208, 1238, 1286 |
| HgaI | GACGC | 4 cut(s) | 554, 1210, 1268, 1349 |
| HhaI | GCGC | 4 cut(s) | 126, 174, 448, 1043 |
| Hin1I | GRCGYC | 4 cut(s) | 42, 1221, 1260, 1341 |
| Hin1II | CATG | 6 cut(s) | 359, 517, 564, 763, 1133, 1388 |
| Hin6I | GCGC | 4 cut(s) | 124, 172, 446, 1041 |
| HinP1I | GCGC | 4 cut(s) | 124, 172, 446, 1041 |
| HincII | GTYRAC | 4 cut(s) | 13, 193, 570, 1333 |
| HindII | GTYRAC | 4 cut(s) | 13, 193, 570, 1333 |
| HphI | GGTGA | 1 cut(s) | 1373 |
| Hpy166II | GTNNAC | 6 cut(s) | 13, 193, 570, 724, 1333, 1381 |
| Hpy188I | TCNGA | 8 cut(s) | 61, 76, 218, 285, 587, 654, 849, 1323 |
| Hpy188III | TCNNGA | 3 cut(s) | 813, 1163, 1273 |
| Hpy8I | GTNNAC | 6 cut(s) | 13, 193, 570, 724, 1333, 1381 |
| Hpy99I | CGWCG | 7 cut(s) | 28, 47, 895, 1064, 1178, 1262, 1343 |
| HpyAV | CCTTC | 5 cut(s) | 139, 218, 511, 992, 1219 |
| HpyCH4III | ACNGT | 6 cut(s) | 24, 80, 101, 106, 539, 1100 |
| HpyCH4IV | ACGT | 7 cut(s) | 42, 168, 176, 187, 380, 572, 1245 |
| HpyCH4V | TGCA | 3 cut(s) | 564, 663, 902 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 322, 617, 665, 690, 929, 968, 1043 |
| HpyF3I | CTNAG | 6 cut(s) | 58, 200, 653, 702, 924, 1033 |
| HpySE526I | ACGT | 7 cut(s) | 42, 168, 176, 187, 380, 572, 1245 |
| Hsp92I | GRCGYC | 4 cut(s) | 42, 1221, 1260, 1341 |
| Hsp92II | CATG | 6 cut(s) | 359, 517, 564, 763, 1133, 1388 |
| HspAI | GCGC | 4 cut(s) | 124, 172, 446, 1041 |
| KroI | GCCGGC | 3 cut(s) | 928, 1037, 1286 |
| KroNI | GCCGGC | 3 cut(s) | 930, 1039, 1288 |
| Ksp22I | TGATCA | 1 cut(s) | 1126 |
| Kzo9I | GATC | 5 cut(s) | 117, 148, 438, 904, 1126 |
| LguI | GCTCTTC | 1 cut(s) | 631 |
| LmnI | GCTCC | 3 cut(s) | 218, 763, 1220 |
| Lsp1109I | GCAGC | 2 cut(s) | 980, 1030 |
| MaeII | ACGT | 7 cut(s) | 42, 168, 176, 187, 380, 572, 1245 |
| MalI | GATC | 5 cut(s) | 119, 150, 440, 906, 1128 |
| MbiI | CCGCTC | 1 cut(s) | 312 |
| MboI | GATC | 5 cut(s) | 117, 148, 438, 904, 1126 |
| MboII | GAAGA | 9 cut(s) | 127, 241, 244, 247, 648, 708, 925, 928, 1097 |
| MflI | RGATCY | 1 cut(s) | 148 |
| MhlI | GDGCHC | 1 cut(s) | 1068 |
| MluCI | AATT | 5 cut(s) | 135, 601, 836, 869, 1324 |
| MluI | ACGCGT | 1 cut(s) | 546 |
| MlyI | GAGTC | 5 cut(s) | 240, 395, 533, 764, 1128 |
| MmeI | TCCRAC | 1 cut(s) | 196 |
| MroNI | GCCGGC | 3 cut(s) | 928, 1037, 1286 |
| MseI | TTAA | 3 cut(s) | 330, 474, 795 |
| MslI | CAYNNNNRTG | 2 cut(s) | 167, 630 |
| MspA1I | CMGCKG | 1 cut(s) | 781 |
| MspR9I | CCNGG | 9 cut(s) | 155, 272, 392, 393, 483, 742, 942, 1141, 1142 |
| MvaI | CCWGG | 2 cut(s) | 742, 942 |
| MvnI | CGCG | 3 cut(s) | 124, 126, 548 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 322, 617, 665, 690, 929, 968, 1043 |
| NaeI | GCCGGC | 3 cut(s) | 930, 1039, 1288 |
| NciI | CCSGG | 7 cut(s) | 155, 272, 392, 393, 483, 1141, 1142 |
| NcoI | CCATGG | 1 cut(s) | 759 |
| NdeII | GATC | 5 cut(s) | 117, 148, 438, 904, 1126 |
| NgoMIV | GCCGGC | 3 cut(s) | 928, 1037, 1286 |
| NlaIII | CATG | 6 cut(s) | 359, 517, 564, 763, 1133, 1388 |
| NlaIV | GGNNCC | 2 cut(s) | 54, 1285 |
| NmeAIII | GCCGAG | 2 cut(s) | 1047, 1264 |
| NsbI | TGCGCA | 1 cut(s) | 173 |
| NspI | RCATGY | 2 cut(s) | 359, 564 |
| NspV | TTCGAA | 1 cut(s) | 1296 |
| PciI | ACATGT | 1 cut(s) | 355 |
| PciSI | GCTCTTC | 1 cut(s) | 631 |
| PdiI | GCCGGC | 3 cut(s) | 930, 1039, 1288 |
| PfeI | GAWTC | 5 cut(s) | 649, 1159, 1169, 1293, 1318 |
| Pfl23II | CGTACG | 1 cut(s) | 177 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 430, 1390 |
| PfoI | TCCNGGA | 3 cut(s) | 153, 270, 740 |
| PinAI | ACCGGT | 1 cut(s) | 501 |
| PkrI | GCNGC | 8 cut(s) | 327, 947, 970, 998, 1026, 1045, 1237, 1345 |
| PleI | GAGTC | 5 cut(s) | 240, 395, 532, 763, 1128 |
| PmaCI | CACGTG | 2 cut(s) | 188, 381 |
| PmlI | CACGTG | 2 cut(s) | 188, 381 |
| PpsI | GAGTC | 5 cut(s) | 240, 395, 532, 763, 1128 |
| Ppu21I | YACGTR | 4 cut(s) | 169, 177, 188, 381 |
| PscI | ACATGT | 1 cut(s) | 355 |
| PshAI | GACNNNNGTC | 1 cut(s) | 188 |
| Psp124BI | GAGCTC | 1 cut(s) | 1068 |
| Psp1406I | AACGTT | 1 cut(s) | 572 |
| Psp6I | CCWGG | 2 cut(s) | 740, 940 |
| PspCI | CACGTG | 2 cut(s) | 188, 381 |
| PspGI | CCWGG | 2 cut(s) | 740, 940 |
| PspLI | CGTACG | 1 cut(s) | 177 |
| PspN4I | GGNNCC | 2 cut(s) | 54, 1285 |
| PspPI | GGNCC | 5 cut(s) | 52, 389, 1143, 1206, 1284 |
| PsuI | RGATCY | 1 cut(s) | 148 |
| PvuII | CAGCTG | 1 cut(s) | 781 |
| RsaI | GTAC | 6 cut(s) | 103, 179, 344, 487, 773, 1198 |
| RsaNI | GTAC | 6 cut(s) | 102, 178, 343, 486, 772, 1197 |
| RseI | CAYNNNNRTG | 2 cut(s) | 167, 630 |
| SacI | GAGCTC | 1 cut(s) | 1068 |
| SalI | GTCGAC | 1 cut(s) | 191 |
| SapI | GCTCTTC | 1 cut(s) | 631 |
| SaqAI | TTAA | 3 cut(s) | 330, 474, 795 |
| SatI | GCNGC | 8 cut(s) | 326, 946, 969, 997, 1025, 1044, 1236, 1344 |
| Sau3AI | GATC | 5 cut(s) | 117, 148, 438, 904, 1126 |
| Sau96I | GGNCC | 5 cut(s) | 52, 389, 1143, 1206, 1284 |
| SchI | GAGTC | 5 cut(s) | 240, 395, 533, 764, 1128 |
| ScrFI | CCNGG | 9 cut(s) | 155, 272, 392, 393, 483, 742, 942, 1141, 1142 |
| SduI | GDGCHC | 1 cut(s) | 1068 |
| SfuI | TTCGAA | 1 cut(s) | 1296 |
| SinI | GGWCC | 1 cut(s) | 52 |
| SmaI | CCCGGG | 2 cut(s) | 393, 1142 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 167, 630 |
| SmlI | CTYRAG | 1 cut(s) | 364 |
| SmoI | CTYRAG | 1 cut(s) | 364 |
| SrfI | GCCCGGGC | 1 cut(s) | 1142 |
| Sse9I | AATT | 5 cut(s) | 135, 601, 836, 869, 1324 |
| SstI | GAGCTC | 1 cut(s) | 1068 |
| StyD4I | CCNGG | 9 cut(s) | 153, 270, 390, 391, 481, 740, 940, 1139, 1140 |
| StyI | CCWWGG | 2 cut(s) | 759, 1263 |
| TaaI | ACNGT | 6 cut(s) | 24, 80, 101, 106, 539, 1100 |
| TaiI | ACGT | 7 cut(s) | 45, 171, 179, 190, 383, 575, 1248 |
| TasI | AATT | 5 cut(s) | 135, 601, 836, 869, 1324 |
| TauI | GCSGC | 6 cut(s) | 328, 948, 999, 1027, 1238, 1346 |
| TfiI | GAWTC | 5 cut(s) | 649, 1159, 1169, 1293, 1318 |
| Tru1I | TTAA | 3 cut(s) | 330, 474, 795 |
| Tru9I | TTAA | 3 cut(s) | 330, 474, 795 |
| TscAI | CASTG | 3 cut(s) | 544, 622, 1103 |
| TseI | GCWGC | 2 cut(s) | 968, 1043 |
| TspDTI | ATGAA | 4 cut(s) | 82, 293, 492, 530 |
| TspGWI | ACGGA | 2 cut(s) | 333, 1162 |
| TspMI | CCCGGG | 2 cut(s) | 391, 1140 |
| TspRI | CASTG | 3 cut(s) | 544, 622, 1103 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 430, 1390 |
| VpaK11BI | GGWCC | 1 cut(s) | 52 |
| XapI | RAATTY | 4 cut(s) | 135, 601, 836, 1324 |
| XceI | RCATGY | 2 cut(s) | 359, 564 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 536 |
| XmaI | CCCGGG | 2 cut(s) | 391, 1140 |
| XmiI | GTMKAC | 1 cut(s) | 192 |
| ZraI | GACGTC | 1 cut(s) | 43 |