Rorug04G0176300

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000808
Physical Location & Seq
Reverse (-)
31014740 .. 31015057
318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0176300.1

Sequence Viewer

Length: 318 bp
ATGAACCAGGTGCCTTCTTTCTTTGTAGACAAGATGAACCACATCCGAGTCAATTTTAAACTCGGATTAGTGAGCCGCTACATTATTGGCCATCATGTGGCCAACAAAATCTCTCAAGAAATCAGCACTCGCCGGTCGGTGATATTTGGGCTCATGATGGAGCTACAGTTCACTCATAGGACTAAAGTGCTGGACAGGTGGGATACACAGTTTTCAGGTTATGCAAAGTTTCAATGTCCGATTGAATTAATGTTTTCTTCAAATAATGTGACCGGAATTACGTTTATAGGTCAGTCCACTGTATGCCCTGAAGTGTAG

Protein Analysis

105

Amino Acids

12.11

Weight (kDa)

9.44

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 10
AccB7I CCANNNNNTGG 1 cut(s) 97
AccI GTMKAC 1 cut(s) 27
AciI CCGC 1 cut(s) 76
AcoI YGGCCR 2 cut(s) 88, 99
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 3 cut(s) 233, 245, 261
AjnI CCWGG 1 cut(s) 6
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 1 cut(s) 163
AluI AGCT 1 cut(s) 163
AoxI GGCC 2 cut(s) 88, 99
AseI ATTAAT 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 151
BalI TGGCCA 2 cut(s) 90, 101
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 153
BccI CCATC 2 cut(s) 99, 151
BciT130I CCWGG 1 cut(s) 8
BciVI GTATCC 1 cut(s) 196
BfmI CTRYAG 1 cut(s) 164
BfuI GTATCC 1 cut(s) 196
BisI GCNGC 1 cut(s) 76
BlsI GCNGC 1 cut(s) 77
Bme1390I CCNGG 1 cut(s) 8
BmiI GGNNCC 1 cut(s) 12
BmrFI CCNGG 1 cut(s) 8
BpuEI CTTGAG 1 cut(s) 99
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse118I RCCGGY 1 cut(s) 132
BseBI CCWGG 1 cut(s) 8
BseGI GGATG 1 cut(s) 42
BseLI CCNNNNNNNGG 1 cut(s) 97
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 2 cut(s) 90, 101
BshNI GGYRCC 1 cut(s) 10
BsiEI CGRYCG 1 cut(s) 137
BsiSI CCGG 2 cut(s) 133, 273
BslI CCNNNNNNNGG 1 cut(s) 97
BsnI GGCC 2 cut(s) 90, 101
Bsp1286I GDGCHC 1 cut(s) 153
BspACI CCGC 1 cut(s) 76
BspANI GGCC 2 cut(s) 90, 101
BspHI TCATGA 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 10
BsrFI RCCGGY 1 cut(s) 132
BssAI RCCGGY 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 3 cut(s) 168, 210, 301
BstF5I GGATG 1 cut(s) 42
BstMCI CGRYCG 1 cut(s) 137
BstNI CCWGG 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 6
BstSFI CTRYAG 1 cut(s) 164
BsuI GTATCC 1 cut(s) 196
BsuRI GGCC 2 cut(s) 90, 101
BtsCI GGATG 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 297
CciI TCATGA 1 cut(s) 153
Cfr10I RCCGGY 1 cut(s) 132
CsiI ACCWGGT 1 cut(s) 6
CviAII CATG 2 cut(s) 95, 154
CviJI RGCY 5 cut(s) 75, 90, 101, 151, 163
CviKI_1 RGCY 5 cut(s) 75, 90, 101, 151, 163
DraI TTTAAA 1 cut(s) 58
EaeI YGGCCR 2 cut(s) 88, 99
Eco24I GRGCYC 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 153
FaeI CATG 2 cut(s) 98, 157
FaiI YATR 6 cut(s) 96, 155, 177, 222, 287, 304
FatI CATG 2 cut(s) 94, 153
FblI GTMKAC 1 cut(s) 27
Fnu4HI GCNGC 1 cut(s) 76
FokI GGATG 1 cut(s) 29
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 1 cut(s) 76
GluI GCNGC 1 cut(s) 76
HaeIII GGCC 2 cut(s) 90, 101
HapII CCGG 2 cut(s) 133, 273
Hin1II CATG 2 cut(s) 98, 157
HinfI GANTC 1 cut(s) 48
HpaII CCGG 2 cut(s) 133, 273
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 3 cut(s) 28, 171, 297
Hpy188I TCNGA 3 cut(s) 47, 65, 240
Hpy188III TCNNGA 2 cut(s) 116, 154
Hpy8I GTNNAC 3 cut(s) 28, 171, 297
HpyAV CCTTC 1 cut(s) 24
HpyCH4III ACNGT 3 cut(s) 168, 210, 301
HpyCH4IV ACGT 1 cut(s) 281
HpyCH4V TGCA 1 cut(s) 224
HpySE526I ACGT 1 cut(s) 281
Hsp92II CATG 2 cut(s) 98, 157
LmnI GCTCC 1 cut(s) 160
LpnPI CCDG 6 cut(s) 20, 146, 176, 181, 201, 286
MabI ACCWGGT 1 cut(s) 6
MaeII ACGT 1 cut(s) 281
MaeIII GTNAC 1 cut(s) 268
MboII GAAGA 1 cut(s) 249
MhlI GDGCHC 1 cut(s) 153
MlsI TGGCCA 2 cut(s) 90, 101
MluCI AATT 3 cut(s) 52, 245, 276
MluNI TGGCCA 2 cut(s) 90, 101
MlyI GAGTC 1 cut(s) 57
Mox20I TGGCCA 2 cut(s) 90, 101
MscI TGGCCA 2 cut(s) 90, 101
MseI TTAA 2 cut(s) 57, 248
Msp20I TGGCCA 2 cut(s) 90, 101
MspI CCGG 2 cut(s) 133, 273
MspR9I CCNGG 1 cut(s) 8
MvaI CCWGG 1 cut(s) 8
NlaIII CATG 2 cut(s) 98, 157
NlaIV GGNNCC 1 cut(s) 12
NmuCI GTSAC 1 cut(s) 268
PagI TCATGA 1 cut(s) 153
PflMI CCANNNNNTGG 1 cut(s) 97
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 6
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 57, 248
SatI GCNGC 1 cut(s) 76
SchI GAGTC 1 cut(s) 57
ScrFI CCNGG 1 cut(s) 8
SduI GDGCHC 1 cut(s) 153
SetI ASST 6 cut(s) 12, 165, 200, 220, 284, 292
SexAI ACCWGGT 1 cut(s) 6
SfcI CTRYAG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 114
SmoI CTYRAG 1 cut(s) 114
Sse9I AATT 3 cut(s) 52, 245, 276
SsiI CCGC 1 cut(s) 76
StyD4I CCNGG 1 cut(s) 6
TaaI ACNGT 3 cut(s) 168, 210, 301
TaiI ACGT 1 cut(s) 284
TasI AATT 3 cut(s) 52, 245, 276
TauI GCSGC 1 cut(s) 78
Tru1I TTAA 2 cut(s) 57, 248
Tru9I TTAA 2 cut(s) 57, 248
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 268
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 2 cut(s) 17, 50
TspRI CASTG 1 cut(s) 304
Van91I CCANNNNNTGG 1 cut(s) 97
VspI ATTAAT 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.