Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_ssc0000252.1

Orthogroup ID

OG0001042

Physical Location & Seq

Genomic Coordinates Forward (+)

242276 .. 243703

Gene Structure 1428 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1428 bp

atggcgtggtgtagacggcggtgtattgcgatattaggagattttgcaactagaaaaacagcggcgggggcaacgtcaacgatagcaagcaagtgggagaaggagacgaagaaaactagcatgttttctgaattaatcagaagaaattctgctactttagcaccacttatggtggaggagaaagacaagaatagaaccatgaaagaagaaagatcgaagagtaagtctctctacttatgttttgaggagcagataactcgttttaatgttgctcacgttattcaatctatagaattgagccacgtgttggcagcaccaactgatggtgatcatggatgcgatgaacttaagttaggtgaggttgcttatagattcggaccggacacttatgttccctgcaggatgagcaatagtgtcatcggctccaaaattattttctccggtggcatggtatttaacagagaggaaagcagtactactcctcctcccaagacgactttgagtaaagaagtttgtgtgtttgagacagctccaagaatagatccaaatccgacattgcagccctcccaagtgtttaagactagtagacccgatcccttcctagcagaggtgggtggcgaactatattgtttcggtggcccatgttactctgagtccttcgaggtatttgacaagacacatgagaaatggttgcctctggatgtgcctcctaagagatatccctacagtgtctgtcatgcaattttgggaaacaaaatgttactctgggtaaaaaatcagaccggagtagtcgggtttgacgtcgctcaaccggaaaaaggatggacagagttggactcttgtttgggaagctgcattccctatgttaatggttatgatcaactatttgtcaagttgaagtctcatcgtggtgatggttttctaatgttctcctgtaatttagaacaacctcagactgtcagagtctttcacatttcgcatgaatgtgattctatgaaacctatggaacctctgcagctgccaaaattgcctcataaatttcctgtaccacaacgatgtcagttttttcatctagaagataataaagtcggccttgttttcttccatgaccgtcgtgagtatttttatgaggattatgattatttttctcttggtgatgatgatgacgatgatgatgatgaggatgatgtgaacgaggaagaggaagaggaagaggaagaggaagaggaagaggaggaggaggaggaggaggaggaggatgttagcagggtgagaactcttgtcataatatttgaatacgaaattgcggagaaatccatggacatcaagtacaaattgctcaccattcgctgcctggaatactctacccaccctcctgaagaaggtaggatcaccgattcaacccttattggcgcttatgtgatctaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00020 ko00190 ko01100 ko01110 ko01120 ko01130 ko01200 ko03015 ko03040 ko04714 ko04932 ko05010 ko05012 ko05016 map00020 map00190 map01100 map01110 map01120 map01130 map01200 map03015 map03040 map04714 map04932 map05010 map05012 map05016

Pfam Domains

Protein Families

RRM_1

Protein Analysis

475

Amino Acids

54.56

Weight (kDa)

4.77

Isoelectric Point (pI)

60.61

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001042)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43023 FvH4_4g00821 FvH4_6g46861 FvH4_6g50642 FvH4_6g50762 FvH4_6g50763
rosa_chinensis	RchiOBHm_Chr2g0172011
rosa_laevigata	RLG00000019866 RLG00000021935 RLG00000021943 RLG00000022032 RLG00000022034 RLG00000022036 RLG00000022038 RLG00000022040 RLG00000022042 RLG00000022044 RLG00000022046 RLG00000022049 RLG00000022051 RLG00000022068 RLG00000022070 RLG00000022080 RLG00000022083 RLG00000022086 RLG00000034562
rosa_multiflora	Rmu_sc0008262.1_g000002 Rmu_sc0012445.1_g000001 Rmu_sc0012468.1_g000010 Rmu_sc0036915.1_g000001 Rmu_ssc0000252.1_g000031 Rmu_ssc0000252.1_g000036 Rmu_ssc0000252.1_g000040
rosa_roxburghii	Rroxscaffold_2G00080270 Rroxscaffold_2G00080490
rosa_rugosa	Rorug02G0547700 Rorug02G0555600 Rorug02G0555600 Rorug02G0555700 Rorug02G0556100
rosa_samantha	Rh2BG643700 Rh2BG644000 Rh2BG644100 Rh2BG644500 Rh2BG644600 Rh2BG645800 Rh2CG610600 Rh2CG610800 Rh2CG611200 Rh2CG611500 Rh2CG611700 Rh2CG612000 Rh2DG658500 Rh2DG658800 Rh2DG658900 Rh2DG659100 Rh2DG659400 Rh2DG659700 Rh2DG660000 Rh4BG019700 Rh5BG331700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	804
AccB7I	CCANNNNNTGG	2 cut(s)	307, 323
AccI	GTMKAC	2 cut(s)	13, 586
AciI	CCGC	4 cut(s)	19, 62, 65, 1307
AclWI	GGATC	3 cut(s)	536, 587, 1397
AcsI	RAATTY	2 cut(s)	145, 1037
AcuI	CTGAAG	1 cut(s)	1398
AcvI	CACGTG	1 cut(s)	304
AcyI	GRCGYC	1 cut(s)	801
AfaI	GTAC	3 cut(s)	475, 1047, 1331
AfiI	CCNNNNNNNGG	5 cut(s)	307, 323, 607, 818, 1382
AflII	CTTAAG	1 cut(s)	347
AflIII	ACRYGT	1 cut(s)	303
AgsI	TTSAA	4 cut(s)	284, 898, 1295, 1401
AhlI	ACTAGT	1 cut(s)	581
AjnI	CCWGG	1 cut(s)	1353
AjuI	GAANNNNNNNTTGG	2 cut(s)	419, 451
AluBI	AGCT	3 cut(s)	530, 852, 1018
AluI	AGCT	3 cut(s)	530, 852, 1018
Alw26I	GTCTC	4 cut(s)	98, 231, 518, 906
AlwI	GGATC	3 cut(s)	536, 587, 1397
AlwNI	CAGNNNCTG	1 cut(s)	732
AoxI	GGCC	2 cut(s)	637, 1090
ApeKI	GCWGC	6 cut(s)	311, 559, 852, 1015, 1018, 1350
ApoI	RAATTY	2 cut(s)	145, 1037
ArsI	GACNNNNNNTTYG	2 cut(s)	209, 241
AseI	ATTAAT	1 cut(s)	134
Asp700I	GAANNNNTTC	1 cut(s)	145
AspLEI	GCGC	1 cut(s)	1415
AspS9I	GGNCC	2 cut(s)	377, 638
AsuHPI	GGTGA	7 cut(s)	338, 368, 923, 1166, 1282, 1333, 1384
AvaII	GGWCC	1 cut(s)	377
BbrPI	CACGTG	1 cut(s)	304
BbvI	GCAGC	6 cut(s)	323, 571, 839, 1005, 1027, 1337
BccI	CCATC	3 cut(s)	317, 816, 908
BceAI	ACGGC	1 cut(s)	32
BcgI	CGANNNNNNTGC	2 cut(s)	239, 273
BciT130I	CCWGG	1 cut(s)	1355
BclI	TGATCA	2 cut(s)	328, 877
BcoDI	GTCTC	4 cut(s)	98, 231, 518, 906
BcuI	ACTAGT	1 cut(s)	581
BfaI	CTAG	5 cut(s)	51, 117, 582, 602, 1073
BfmI	CTRYAG	4 cut(s)	288, 397, 724, 1013
BfoI	RGCGCY	1 cut(s)	1416
BfrI	CTTAAG	1 cut(s)	347
BisI	GCNGC	7 cut(s)	63, 312, 560, 853, 1016, 1019, 1351
BlsI	GCNGC	7 cut(s)	64, 313, 561, 854, 1017, 1020, 1352
BmcAI	AGTACT	1 cut(s)	475
Bme1390I	CCNGG	1 cut(s)	1355
Bme18I	GGWCC	1 cut(s)	377
BmgT120I	GGNCC	2 cut(s)	377, 638
BmiI	GGNNCC	2 cut(s)	424, 1008
BmrFI	CCNGG	1 cut(s)	1355
BmsI	GCATC	1 cut(s)	326
BplI	GAGNNNNNCTC	4 cut(s)	211, 243, 821, 853
BsaAI	YACGTR	1 cut(s)	304
BsaBI	GATNNNNATC	2 cut(s)	327, 546
BsaHI	GRCGYC	1 cut(s)	801
BsaJI	CCNNGG	1 cut(s)	1317
BsaWI	WCCGGW	4 cut(s)	379, 440, 782, 811
BsaXI	ACNNNNNCTCC	6 cut(s)	466, 469, 496, 499, 1357, 1387
Bsc4I	CCNNNNNNNGG	5 cut(s)	307, 323, 607, 818, 1382
Bse3DI	GCAATG	1 cut(s)	554
Bse8I	GATNNNNATC	2 cut(s)	327, 546
BseBI	CCWGG	1 cut(s)	1355
BseDI	CCNNGG	1 cut(s)	1317
BseGI	GGATG	6 cut(s)	341, 408, 706, 827, 1189, 1264
BseJI	GATNNNNATC	2 cut(s)	327, 546
BseLI	CCNNNNNNNGG	5 cut(s)	307, 323, 607, 818, 1382
BseMI	GCAATG	1 cut(s)	554
BseMII	CTCAG	2 cut(s)	642, 965
BseXI	GCAGC	6 cut(s)	323, 571, 839, 1005, 1027, 1337
BshFI	GGCC	2 cut(s)	639, 1092
BsiSI	CCGG	4 cut(s)	380, 441, 783, 812
BslI	CCNNNNNNNGG	5 cut(s)	307, 323, 607, 818, 1382
BsmAI	GTCTC	4 cut(s)	98, 231, 518, 906
BsmBI	CGTCTC	1 cut(s)	98
BsmI	GAATGC	1 cut(s)	855
BsnI	GGCC	2 cut(s)	639, 1092
Bsp143I	GATC	7 cut(s)	212, 328, 541, 592, 877, 1389, 1422
Bsp19I	CCATGG	1 cut(s)	1317
BspACI	CCGC	4 cut(s)	19, 62, 65, 1307
BspANI	GGCC	2 cut(s)	639, 1092
BspCNI	CTCAG	2 cut(s)	643, 964
BspLI	GGNNCC	2 cut(s)	424, 1008
BspMAI	CTGCAG	2 cut(s)	401, 1017
BspPI	GGATC	3 cut(s)	536, 587, 1397
BspTI	CTTAAG	1 cut(s)	347
BsrDI	GCAATG	1 cut(s)	554
BssECI	CCNNGG	1 cut(s)	1317
BssMI	GATC	7 cut(s)	212, 328, 541, 592, 877, 1389, 1422
BssNI	GRCGYC	1 cut(s)	801
BssT1I	CCWWGG	1 cut(s)	1317
Bst2UI	CCWGG	1 cut(s)	1355
Bst4CI	ACNGT	3 cut(s)	728, 958, 1112
Bst6I	CTCTTC	7 cut(s)	212, 1194, 1200, 1206, 1212, 1218, 1224
BstACI	GRCGYC	1 cut(s)	801
BstAFI	CTTAAG	1 cut(s)	347
BstBAI	YACGTR	1 cut(s)	304
BstC8I	GCNNGC	1 cut(s)	88
BstDEI	CTNAG	3 cut(s)	651, 711, 951
BstDSI	CCRYGG	1 cut(s)	1317
BstENI	CCTNNNNNAGG	2 cut(s)	605, 1380
BstF5I	GGATG	6 cut(s)	341, 408, 706, 827, 1189, 1264
BstH2I	RGCGCY	1 cut(s)	1416
BstHHI	GCGC	1 cut(s)	1415
BstKTI	GATC	7 cut(s)	215, 331, 544, 595, 880, 1392, 1425
BstMAI	GTCTC	4 cut(s)	98, 231, 518, 906
BstMBI	GATC	7 cut(s)	212, 328, 541, 592, 877, 1389, 1422
BstMWI	GCNNNNNNNGC	4 cut(s)	68, 158, 405, 1027
BstNI	CCWGG	1 cut(s)	1355
BstNSI	RCATGY	1 cut(s)	124
BstSCI	CCNGG	1 cut(s)	1353
BstSFI	CTRYAG	4 cut(s)	288, 397, 724, 1013
BstV1I	GCAGC	6 cut(s)	323, 571, 839, 1005, 1027, 1337
BstX2I	RGATCY	1 cut(s)	541
BstYI	RGATCY	1 cut(s)	541
BsuRI	GGCC	2 cut(s)	639, 1092
BtgI	CCRYGG	1 cut(s)	1317
BtgZI	GCGATG	1 cut(s)	354
BtsCI	GGATG	6 cut(s)	341, 408, 706, 827, 1189, 1264
BtsIMutI	CAGTG	1 cut(s)	733
Cac8I	GCNNGC	1 cut(s)	88
CaiI	CAGNNNCTG	1 cut(s)	732
CfoI	GCGC	1 cut(s)	1415
Cfr13I	GGNCC	2 cut(s)	377, 638
CpoI	CGGWCCG	1 cut(s)	377
Csp6I	GTAC	3 cut(s)	474, 1046, 1330
CspI	CGGWCCG	1 cut(s)	377
CviJI	RGCY	8 cut(s)	300, 423, 530, 562, 639, 852, 1018, 1092
CviKI_1	RGCY	8 cut(s)	300, 423, 530, 562, 639, 852, 1018, 1092
CviQI	GTAC	3 cut(s)	474, 1046, 1330
DdeI	CTNAG	3 cut(s)	651, 711, 951
DpnI	GATC	7 cut(s)	214, 330, 543, 594, 879, 1391, 1424
DpnII	GATC	7 cut(s)	212, 328, 541, 592, 877, 1389, 1422
Eam1104I	CTCTTC	7 cut(s)	212, 1194, 1200, 1206, 1212, 1218, 1224
EarI	CTCTTC	7 cut(s)	212, 1194, 1200, 1206, 1212, 1218, 1224
Eco130I	CCWWGG	1 cut(s)	1317
Eco32I	GATATC	1 cut(s)	719
Eco47I	GGWCC	1 cut(s)	377
Eco57I	CTGAAG	1 cut(s)	1398
Eco72I	CACGTG	1 cut(s)	304
EcoNI	CCTNNNNNAGG	2 cut(s)	605, 1380
EcoRII	CCWGG	1 cut(s)	1353
EcoRV	GATATC	1 cut(s)	719
EcoT14I	CCWWGG	1 cut(s)	1317
ErhI	CCWWGG	1 cut(s)	1317
Esp3I	CGTCTC	1 cut(s)	98
FalI	AAGNNNNNCTT	2 cut(s)	1077, 1109
FauI	CCCGC	1 cut(s)	58
FbaI	TGATCA	2 cut(s)	328, 877
FblI	GTMKAC	2 cut(s)	13, 586
Fnu4HI	GCNGC	7 cut(s)	63, 312, 560, 853, 1016, 1019, 1351
FokI	GGATG	6 cut(s)	348, 415, 713, 834, 1196, 1271
Fsp4HI	GCNGC	7 cut(s)	63, 312, 560, 853, 1016, 1019, 1351
FspBI	CTAG	5 cut(s)	51, 117, 582, 602, 1073
GlaI	GCGC	1 cut(s)	1414
GluI	GCNGC	7 cut(s)	63, 312, 560, 853, 1016, 1019, 1351
HaeII	RGCGCY	1 cut(s)	1416
HaeIII	GGCC	2 cut(s)	639, 1092
HapII	CCGG	4 cut(s)	380, 441, 783, 812
HhaI	GCGC	1 cut(s)	1415
Hin1I	GRCGYC	1 cut(s)	801
Hin6I	GCGC	1 cut(s)	1413
HinP1I	GCGC	1 cut(s)	1413
HincII	GTYRAC	1 cut(s)	78
HindII	GTYRAC	1 cut(s)	78
HinfI	GANTC	6 cut(s)	372, 653, 836, 963, 989, 1397
HpaII	CCGG	4 cut(s)	380, 441, 783, 812
HphI	GGTGA	7 cut(s)	338, 368, 923, 1166, 1282, 1333, 1384
Hpy166II	GTNNAC	4 cut(s)	14, 78, 587, 1192
Hpy188I	TCNGA	8 cut(s)	130, 140, 377, 552, 652, 780, 954, 962
Hpy188III	TCNNGA	4 cut(s)	698, 1073, 1115, 1376
Hpy8I	GTNNAC	4 cut(s)	14, 78, 587, 1192
Hpy99I	CGWCG	2 cut(s)	806, 1116
HpyAV	CCTTC	4 cut(s)	94, 607, 667, 1376
HpyCH4III	ACNGT	3 cut(s)	728, 958, 1112
HpyCH4IV	ACGT	4 cut(s)	74, 276, 303, 801
HpyCH4V	TGCA	6 cut(s)	47, 399, 559, 740, 855, 1015
HpyF10VI	GCNNNNNNNGC	4 cut(s)	68, 158, 405, 1027
HpyF3I	CTNAG	3 cut(s)	651, 711, 951
HpySE526I	ACGT	4 cut(s)	74, 276, 303, 801
Hsp92I	GRCGYC	1 cut(s)	801
HspAI	GCGC	1 cut(s)	1413
Ksp22I	TGATCA	2 cut(s)	328, 877
Kzo9I	GATC	7 cut(s)	212, 328, 541, 592, 877, 1389, 1422
LmnI	GCTCC	3 cut(s)	247, 428, 535
Lsp1109I	GCAGC	6 cut(s)	323, 571, 839, 1005, 1027, 1337
LweI	GCATC	1 cut(s)	326
MaeI	CTAG	5 cut(s)	51, 117, 582, 602, 1073
MaeII	ACGT	4 cut(s)	74, 276, 303, 801
MaeIII	GTNAC	2 cut(s)	644, 759
MalI	GATC	7 cut(s)	214, 330, 543, 594, 879, 1391, 1424
MboI	GATC	7 cut(s)	212, 328, 541, 592, 877, 1389, 1422
MflI	RGATCY	1 cut(s)	541
MlyI	GAGTC	3 cut(s)	662, 830, 972
MmeI	TCCRAC	2 cut(s)	575, 813
MroXI	GAANNNNTTC	1 cut(s)	145
MseI	TTAA	6 cut(s)	134, 264, 348, 456, 576, 867
MslI	CAYNNNNRTG	3 cut(s)	909, 984, 1054
MspA1I	CMGCKG	2 cut(s)	62, 1018
MspCI	CTTAAG	1 cut(s)	347
MspI	CCGG	4 cut(s)	380, 441, 783, 812
MspR9I	CCNGG	1 cut(s)	1355
Mva1269I	GAATGC	1 cut(s)	855
MvaI	CCWGG	1 cut(s)	1355
MwoI	GCNNNNNNNGC	4 cut(s)	68, 158, 405, 1027
NcoI	CCATGG	1 cut(s)	1317
NdeII	GATC	7 cut(s)	212, 328, 541, 592, 877, 1389, 1422
NlaIV	GGNNCC	2 cut(s)	424, 1008
NspI	RCATGY	1 cut(s)	124
PcsI	WCGNNNNNNNCGW	1 cut(s)	798
PctI	GAATGC	1 cut(s)	855
PdmI	GAANNNNTTC	1 cut(s)	145
PfeI	GAWTC	3 cut(s)	372, 989, 1397
PflMI	CCANNNNNTGG	2 cut(s)	307, 323
PkrI	GCNGC	7 cut(s)	64, 313, 561, 854, 1017, 1020, 1352
PleI	GAGTC	3 cut(s)	661, 830, 971
PmaCI	CACGTG	1 cut(s)	304
PmlI	CACGTG	1 cut(s)	304
PpsI	GAGTC	3 cut(s)	661, 830, 971
Ppu21I	YACGTR	1 cut(s)	304
PshBI	ATTAAT	1 cut(s)	134
Psp6I	CCWGG	1 cut(s)	1353
PspCI	CACGTG	1 cut(s)	304
PspGI	CCWGG	1 cut(s)	1353
PspN4I	GGNNCC	2 cut(s)	424, 1008
PspPI	GGNCC	2 cut(s)	377, 638
PstI	CTGCAG	2 cut(s)	401, 1017
PstNI	CAGNNNCTG	1 cut(s)	732
PsuI	RGATCY	1 cut(s)	541
PvuII	CAGCTG	1 cut(s)	1018
RsaI	GTAC	3 cut(s)	475, 1047, 1331
RsaNI	GTAC	3 cut(s)	474, 1046, 1330
RseI	CAYNNNNRTG	3 cut(s)	909, 984, 1054
Rsr2I	CGGWCCG	1 cut(s)	377
RsrII	CGGWCCG	1 cut(s)	377
SaqAI	TTAA	6 cut(s)	134, 264, 348, 456, 576, 867
SatI	GCNGC	7 cut(s)	63, 312, 560, 853, 1016, 1019, 1351
Sau3AI	GATC	7 cut(s)	212, 328, 541, 592, 877, 1389, 1422
Sau96I	GGNCC	2 cut(s)	377, 638
SbfI	CCTGCAGG	1 cut(s)	401
ScaI	AGTACT	1 cut(s)	475
SchI	GAGTC	3 cut(s)	662, 830, 972
ScrFI	CCNGG	1 cut(s)	1355
SdaI	CCTGCAGG	1 cut(s)	401
SfaNI	GCATC	1 cut(s)	326
SfcI	CTRYAG	4 cut(s)	288, 397, 724, 1013
SinI	GGWCC	1 cut(s)	377
SmiMI	CAYNNNNRTG	3 cut(s)	909, 984, 1054
SmlI	CTYRAG	1 cut(s)	347
SmoI	CTYRAG	1 cut(s)	347
SpeI	ACTAGT	1 cut(s)	581
Sse8387I	CCTGCAGG	1 cut(s)	401
SsiI	CCGC	4 cut(s)	19, 62, 65, 1307
SspI	AATATT	1 cut(s)	1290
SspMI	CTAG	5 cut(s)	51, 117, 582, 602, 1073
StyD4I	CCNGG	1 cut(s)	1353
StyI	CCWWGG	1 cut(s)	1317
TaaI	ACNGT	3 cut(s)	728, 958, 1112
TaiI	ACGT	4 cut(s)	77, 279, 306, 804
TaqI	TCGA	2 cut(s)	215, 660
TatI	WGTACW	2 cut(s)	473, 1329
TauI	GCSGC	1 cut(s)	65
TfiI	GAWTC	3 cut(s)	372, 989, 1397
Tru1I	TTAA	6 cut(s)	134, 264, 348, 456, 576, 867
Tru9I	TTAA	6 cut(s)	134, 264, 348, 456, 576, 867
TscAI	CASTG	1 cut(s)	733
TseI	GCWGC	6 cut(s)	311, 559, 852, 1015, 1018, 1350
TspDTI	ATGAA	5 cut(s)	215, 357, 996, 1010, 1058
TspRI	CASTG	1 cut(s)	733
Van91I	CCANNNNNTGG	2 cut(s)	307, 323
Vha464I	CTTAAG	1 cut(s)	347
VpaK11BI	GGWCC	1 cut(s)	377
VspI	ATTAAT	1 cut(s)	134
XagI	CCTNNNNNAGG	2 cut(s)	605, 1380
XapI	RAATTY	2 cut(s)	145, 1037
XbaI	TCTAGA	1 cut(s)	1072
XceI	RCATGY	1 cut(s)	124
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1351
XmiI	GTMKAC	2 cut(s)	13, 586
XmnI	GAANNNNTTC	1 cut(s)	145
XspI	CTAG	5 cut(s)	51, 117, 582, 602, 1073
ZraI	GACGTC	1 cut(s)	802
ZrmI	AGTACT	1 cut(s)	475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.