Rroxscaffold_2G00087680

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001772
Physical Location & Seq
Forward (+)
9678392 .. 9689737
11346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087680.1

Sequence Viewer

Length: 1020 bp
ATGGCGACCTCGCTAGAGGTTCTGCTCTTGCTGGTCCGAGTTCTTCGGGATGGAGGGTGGTGGGTTGGGCCTTTGGGCTTCAGCCCATCTTTCTTTCTCTATCCGGCTTGGGCTTGGCTTGAGCCGAGTCTTTCAAGGGTACAACCTCGAATAGAATCTAATTCGGTCGCTTCGTTCTTCCGGACCATTCCAAAGTCACCGGCTCCACGCGGCGTGTTCCCGGATCCGCACCGCTCGCCCGCTCCCTTGCTTCACTGCGGCGCGCTCTTAGCAACTCGACGAGGACCGGAGGTTCGGAGAGTATCAAAGGAAGCACGAGCTATTAGCTCTCCCGGAGTTACTAACCTCGATCCTCATGTCCCATTTGTGGTTAGCCGGGAGAATAATGTGCAATGTGTGTCCGTTCCCGACGATCTTTGGGCATTGTCCCCCGAGGAATCTATGATTGGTGAAGATATTTCAGTAATTGAAAAGGTGGTTATTGCTGCTGATTGCTATGCAGAGAAGACTTTTGCTGAAAATAGTGATATTTCACATACCGATGATTCTCCTTCCCATAATGAGGAAGGCATGGTTTCGATGCTTAGGGATCAACCCCTCCCCCTCTTTTTAGCTTCAATGTTTAAATTATTATGTTGGAATGTGCGTGGAGCTGGAGGTAAAAAGTTTAGATCAGCTATTATCGATCTCATCCATCCGCATAAGATGGATGTTTTGATTGTTTGTGAACCTAGGGTTCCTTATGCAAGGGTCAAGAATTTTCTTAGCAGAAATGGATTTCCGGATGCTGAAGTTATGGAGGCCCGCGGGTTTTCGGGAGGTGTGTGGATTCTTTATGATAGGAACAAAATTGGAGTCACTTTTATTGACGTAATATTCAATCATCATTGTTCGATTTCGATAAGTGGTTATCAAGACCGGGTTCTCTCTGCTATCTATGCTAGTCCTACTAATTCTATTAGAAGTGTGCTCGGGATTACTTATCTTCCTTTGCTAAACAATGCAAGCTGCCTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

339

Amino Acids

37.5

Weight (kDa)

6.75

Isoelectric Point (pI)

53.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 234, 242
AccII CGCG 3 cut(s) 210, 263, 807
AccIII TCCGGA 2 cut(s) 180, 781
AciI CCGC 8 cut(s) 210, 227, 232, 240, 258, 698, 805, 807
AclWI GGATC 4 cut(s) 218, 231, 344, 597
AcsI RAATTY 1 cut(s) 757
AcuI CTGAAG 2 cut(s) 64, 810
AfaI GTAC 1 cut(s) 141
AfiI CCNNNNNNNGG 2 cut(s) 367, 562
AgsI TTSAA 4 cut(s) 135, 470, 618, 880
AjuI GAANNNNNNNTTGG 2 cut(s) 429, 461
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
AluBI AGCT 6 cut(s) 320, 327, 614, 653, 677, 1008
AluI AGCT 6 cut(s) 320, 327, 614, 653, 677, 1008
Alw21I GWGCWC 1 cut(s) 972
AlwI GGATC 4 cut(s) 218, 231, 344, 597
Ama87I CYCGRG 2 cut(s) 431, 971
Aor13HI TCCGGA 2 cut(s) 180, 781
AoxI GGCC 2 cut(s) 68, 801
ApeKI GCWGC 2 cut(s) 485, 1008
ApoI RAATTY 1 cut(s) 757
ArsI GACNNNNNNTTYG 4 cut(s) 276, 308, 840, 872
AspA2I CCTAGG 1 cut(s) 731
AspLEI GCGC 2 cut(s) 263, 265
AspS9I GGNCC 5 cut(s) 34, 68, 183, 284, 802
AsuC2I CCSGG 4 cut(s) 221, 333, 377, 920
AsuHPI GGTGA 2 cut(s) 189, 461
AvaI CYCGRG 2 cut(s) 431, 971
AvaII GGWCC 3 cut(s) 34, 183, 284
AvrII CCTAGG 1 cut(s) 731
BamHI GGATCC 1 cut(s) 223
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 512
Bbv12I GWGCWC 1 cut(s) 972
BbvI GCAGC 2 cut(s) 472, 995
BccI CCATC 4 cut(s) 44, 94, 700, 702
BcnI CCSGG 4 cut(s) 221, 333, 377, 920
BfaI CTAG 3 cut(s) 14, 732, 942
BisI GCNGC 4 cut(s) 211, 259, 486, 1009
BlnI CCTAGG 1 cut(s) 731
BlsI GCNGC 4 cut(s) 212, 260, 487, 1010
Bme1390I CCNGG 4 cut(s) 221, 333, 377, 920
Bme18I GGWCC 3 cut(s) 34, 183, 284
BmeT110I CYCGRG 2 cut(s) 431, 971
BmgT120I GGNCC 5 cut(s) 34, 68, 183, 284, 802
BmiI GGNNCC 3 cut(s) 204, 225, 738
BmrFI CCNGG 4 cut(s) 221, 333, 377, 920
BmsI GCATC 2 cut(s) 570, 775
BpiI GAAGAC 1 cut(s) 512
BpmI CTGGAG 1 cut(s) 675
Bpu10I CCTNAGC 1 cut(s) 584
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 4 cut(s) 221, 333, 377, 920
Bsa29I ATCGAT 1 cut(s) 684
BsaJI CCNNGG 4 cut(s) 432, 731, 805, 1012
BsaWI WCCGGW 3 cut(s) 180, 286, 781
Bsc4I CCNNNNNNNGG 2 cut(s) 367, 562
Bse118I RCCGGY 1 cut(s) 199
Bse3DI GCAATG 1 cut(s) 398
BseAI TCCGGA 2 cut(s) 180, 781
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 4 cut(s) 432, 731, 805, 1012
BseGI GGATG 5 cut(s) 55, 690, 694, 715, 790
BseLI CCNNNNNNNGG 2 cut(s) 367, 562
BseMI GCAATG 1 cut(s) 398
BsePI GCGCGC 1 cut(s) 261
BseXI GCAGC 2 cut(s) 472, 995
Bsh1236I CGCG 3 cut(s) 210, 263, 807
Bsh1285I CGRYCG 1 cut(s) 168
BshFI GGCC 2 cut(s) 70, 803
BshVI ATCGAT 1 cut(s) 684
BsiEI CGRYCG 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 972
BsiHKCI CYCGRG 2 cut(s) 431, 971
BsiSI CCGG 9 cut(s) 104, 181, 200, 221, 287, 333, 376, 782, 919
BslFI GGGAC 2 cut(s) 344, 412
BslI CCNNNNNNNGG 2 cut(s) 367, 562
BsmFI GGGAC 2 cut(s) 344, 412
BsnI GGCC 2 cut(s) 70, 803
BsoBI CYCGRG 2 cut(s) 431, 971
Bsp1286I GDGCHC 1 cut(s) 972
Bsp13I TCCGGA 2 cut(s) 180, 781
Bsp143I GATC 6 cut(s) 223, 349, 412, 589, 671, 685
BspACI CCGC 8 cut(s) 210, 227, 232, 240, 258, 698, 805, 807
BspANI GGCC 2 cut(s) 70, 803
BspDI ATCGAT 1 cut(s) 684
BspEI TCCGGA 2 cut(s) 180, 781
BspFNI CGCG 3 cut(s) 210, 263, 807
BspLI GGNNCC 3 cut(s) 204, 225, 738
BspPI GGATC 4 cut(s) 218, 231, 344, 597
BsrBI CCGCTC 2 cut(s) 234, 242
BsrDI GCAATG 1 cut(s) 398
BsrFI RCCGGY 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 4 cut(s) 432, 731, 805, 1012
BssHII GCGCGC 1 cut(s) 261
BssMI GATC 6 cut(s) 223, 349, 412, 589, 671, 685
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 2 cut(s) 731, 1012
Bst2BI CACGAG 1 cut(s) 315
BstC8I GCNNGC 5 cut(s) 236, 240, 263, 805, 1006
BstDEI CTNAG 3 cut(s) 268, 584, 764
BstDSI CCRYGG 1 cut(s) 805
BstF5I GGATG 5 cut(s) 55, 690, 694, 715, 790
BstFNI CGCG 3 cut(s) 210, 263, 807
BstHHI GCGC 2 cut(s) 263, 265
BstKTI GATC 6 cut(s) 226, 352, 415, 592, 674, 688
BstMBI GATC 6 cut(s) 223, 349, 412, 589, 671, 685
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 3 cut(s) 235, 269, 938
BstSCI CCNGG 4 cut(s) 219, 331, 375, 918
BstUI CGCG 3 cut(s) 210, 263, 807
BstV1I GCAGC 2 cut(s) 472, 995
BstV2I GAAGAC 1 cut(s) 512
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 684
BsuRI GGCC 2 cut(s) 70, 803
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 1 cut(s) 805
BtsCI GGATG 5 cut(s) 55, 690, 694, 715, 790
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 1 cut(s) 253
Cac8I GCNNGC 5 cut(s) 236, 240, 263, 805, 1006
CfoI GCGC 2 cut(s) 263, 265
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 5 cut(s) 34, 68, 183, 284, 802
Cfr42I CCGCGG 1 cut(s) 808
ClaI ATCGAT 1 cut(s) 684
Csp6I GTAC 1 cut(s) 140
CviAII CATG 2 cut(s) 356, 571
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 3 cut(s) 268, 584, 764
DpnI GATC 6 cut(s) 225, 351, 414, 591, 673, 687
DpnII GATC 6 cut(s) 223, 349, 412, 589, 671, 685
DraI TTTAAA 1 cut(s) 625
Eco130I CCWWGG 2 cut(s) 731, 1012
Eco47I GGWCC 3 cut(s) 34, 183, 284
Eco57I CTGAAG 2 cut(s) 64, 810
Eco88I CYCGRG 2 cut(s) 431, 971
EcoT14I CCWWGG 2 cut(s) 731, 1012
ErhI CCWWGG 2 cut(s) 731, 1012
FaeI CATG 2 cut(s) 359, 574
FaqI GGGAC 2 cut(s) 344, 412
FatI CATG 2 cut(s) 355, 570
FauI CCCGC 3 cut(s) 247, 800, 812
Fnu4HI GCNGC 4 cut(s) 211, 259, 486, 1009
FokI GGATG 5 cut(s) 62, 677, 681, 722, 797
Fsp4HI GCNGC 4 cut(s) 211, 259, 486, 1009
FspBI CTAG 3 cut(s) 14, 732, 942
GlaI GCGC 2 cut(s) 262, 264
GluI GCNGC 4 cut(s) 211, 259, 486, 1009
GsuI CTGGAG 1 cut(s) 675
HaeIII GGCC 2 cut(s) 70, 803
HapII CCGG 9 cut(s) 104, 181, 200, 221, 287, 333, 376, 782, 919
HhaI GCGC 2 cut(s) 263, 265
Hin1II CATG 2 cut(s) 359, 574
Hin6I GCGC 2 cut(s) 261, 263
HinP1I GCGC 2 cut(s) 261, 263
HinfI GANTC 6 cut(s) 127, 155, 437, 545, 829, 855
HpaII CCGG 9 cut(s) 104, 181, 200, 221, 287, 333, 376, 782, 919
HphI GGTGA 2 cut(s) 189, 461
Hpy166II GTNNAC 1 cut(s) 728
Hpy188I TCNGA 2 cut(s) 38, 297
Hpy188III TCNNGA 8 cut(s) 47, 181, 407, 754, 782, 816, 914, 973
Hpy8I GTNNAC 1 cut(s) 728
Hpy99I CGWCG 2 cut(s) 282, 413
HpyAV CCTTC 2 cut(s) 560, 561
HpyCH4IV ACGT 1 cut(s) 870
HpyCH4V TGCA 4 cut(s) 391, 500, 746, 1004
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 269, 938
HpyF3I CTNAG 3 cut(s) 268, 584, 764
HpySE526I ACGT 1 cut(s) 870
Hsp92II CATG 2 cut(s) 359, 574
HspAI GCGC 2 cut(s) 261, 263
Kpn2I TCCGGA 2 cut(s) 180, 781
KspI CCGCGG 1 cut(s) 808
Kzo9I GATC 6 cut(s) 223, 349, 412, 589, 671, 685
LmnI GCTCC 3 cut(s) 208, 247, 650
Lsp1109I GCAGC 2 cut(s) 472, 995
LweI GCATC 2 cut(s) 570, 775
MaeI CTAG 3 cut(s) 14, 732, 942
MaeII ACGT 1 cut(s) 870
MaeIII GTNAC 3 cut(s) 195, 337, 856
MalI GATC 6 cut(s) 225, 351, 414, 591, 673, 687
MbiI CCGCTC 2 cut(s) 234, 242
MboI GATC 6 cut(s) 223, 349, 412, 589, 671, 685
MboII GAAGA 5 cut(s) 35, 169, 464, 517, 977
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 972
MluCI AATT 6 cut(s) 160, 465, 626, 757, 849, 952
MlyI GAGTC 2 cut(s) 136, 864
MmeI TCCRAC 1 cut(s) 617
MroI TCCGGA 2 cut(s) 180, 781
MseI TTAA 2 cut(s) 624, 1018
MslI CAYNNNNRTG 1 cut(s) 540
MspA1I CMGCKG 1 cut(s) 807
MspI CCGG 9 cut(s) 104, 181, 200, 221, 287, 333, 376, 782, 919
MspR9I CCNGG 4 cut(s) 221, 333, 377, 920
MvnI CGCG 3 cut(s) 210, 263, 807
MwoI GCNNNNNNNGC 3 cut(s) 235, 269, 938
NciI CCSGG 4 cut(s) 221, 333, 377, 920
NdeII GATC 6 cut(s) 223, 349, 412, 589, 671, 685
NlaIII CATG 2 cut(s) 359, 574
NlaIV GGNNCC 3 cut(s) 204, 225, 738
NmeAIII GCCGAG 1 cut(s) 150
NmuCI GTSAC 2 cut(s) 195, 856
PauI GCGCGC 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 170
PfeI GAWTC 4 cut(s) 155, 437, 545, 829
PfoI TCCNGGA 2 cut(s) 219, 331
PkrI GCNGC 4 cut(s) 212, 260, 487, 1010
PleI GAGTC 2 cut(s) 135, 863
PpsI GAGTC 2 cut(s) 135, 863
PspN4I GGNNCC 3 cut(s) 204, 225, 738
PspPI GGNCC 5 cut(s) 34, 68, 183, 284, 802
PsuI RGATCY 1 cut(s) 223
PteI GCGCGC 1 cut(s) 261
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
RseI CAYNNNNRTG 1 cut(s) 540
SacII CCGCGG 1 cut(s) 808
SaqAI TTAA 2 cut(s) 624, 1018
SatI GCNGC 4 cut(s) 211, 259, 486, 1009
Sau3AI GATC 6 cut(s) 223, 349, 412, 589, 671, 685
Sau96I GGNCC 5 cut(s) 34, 68, 183, 284, 802
SchI GAGTC 2 cut(s) 136, 864
ScrFI CCNGG 4 cut(s) 221, 333, 377, 920
SduI GDGCHC 1 cut(s) 972
SfaNI GCATC 2 cut(s) 570, 775
Sfr303I CCGCGG 1 cut(s) 808
SgrBI CCGCGG 1 cut(s) 808
SinI GGWCC 3 cut(s) 34, 183, 284
SmiMI CAYNNNNRTG 1 cut(s) 540
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 6 cut(s) 160, 465, 626, 757, 849, 952
SsiI CCGC 8 cut(s) 210, 227, 232, 240, 258, 698, 805, 807
SspI AATATT 1 cut(s) 876
SspMI CTAG 3 cut(s) 14, 732, 942
StyD4I CCNGG 4 cut(s) 219, 331, 375, 918
StyI CCWWGG 2 cut(s) 731, 1012
TaiI ACGT 1 cut(s) 873
TaqI TCGA 7 cut(s) 148, 277, 348, 578, 684, 893, 899
TaqII GACCGA 1 cut(s) 154
TasI AATT 6 cut(s) 160, 465, 626, 757, 849, 952
TauI GCSGC 2 cut(s) 213, 261
TfiI GAWTC 4 cut(s) 155, 437, 545, 829
Tru1I TTAA 2 cut(s) 624, 1018
Tru9I TTAA 2 cut(s) 624, 1018
TscAI CASTG 1 cut(s) 260
TseFI GTSAC 2 cut(s) 195, 856
TseI GCWGC 2 cut(s) 485, 1008
Tsp45I GTSAC 2 cut(s) 195, 856
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 1 cut(s) 260
VpaK11BI GGWCC 3 cut(s) 34, 183, 284
XapI RAATTY 1 cut(s) 757
XmaJI CCTAGG 1 cut(s) 731
XspI CTAG 3 cut(s) 14, 732, 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.