Component of the DNA topoisomerase VI involved in chromatin organization and progression of endoreduplication cycles. Relaxes both positive and negative superturns and exhibits a strong decatenase activity. The B subunit binds ATP
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 322 |
| Acc36I | ACCTGC | 1 cut(s) | 28 |
| AccI | GTMKAC | 1 cut(s) | 269 |
| AgsI | TTSAA | 2 cut(s) | 32, 73 |
| AluBI | AGCT | 3 cut(s) | 45, 149, 286 |
| AluI | AGCT | 3 cut(s) | 45, 149, 286 |
| AseI | ATTAAT | 1 cut(s) | 57 |
| BfuAI | ACCTGC | 1 cut(s) | 28 |
| BmsI | GCATC | 1 cut(s) | 234 |
| BpmI | CTGGAG | 1 cut(s) | 129 |
| BpuEI | CTTGAG | 2 cut(s) | 152, 272 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 233, 263 |
| Bse3DI | GCAATG | 1 cut(s) | 174 |
| BseMI | GCAATG | 1 cut(s) | 174 |
| BseMII | CTCAG | 2 cut(s) | 141, 153 |
| BsiSI | CCGG | 1 cut(s) | 104 |
| BspCNI | CTCAG | 2 cut(s) | 142, 154 |
| BspMI | ACCTGC | 1 cut(s) | 28 |
| BspQI | GCTCTTC | 1 cut(s) | 68 |
| BsrDI | GCAATG | 1 cut(s) | 174 |
| Bst4CI | ACNGT | 1 cut(s) | 125 |
| Bst6I | CTCTTC | 1 cut(s) | 68 |
| BstC8I | GCNNGC | 1 cut(s) | 284 |
| BstDEI | CTNAG | 3 cut(s) | 150, 162, 278 |
| BtsIMutI | CAGTG | 1 cut(s) | 130 |
| BveI | ACCTGC | 1 cut(s) | 28 |
| Cac8I | GCNNGC | 1 cut(s) | 284 |
| CviJI | RGCY | 4 cut(s) | 45, 115, 149, 286 |
| CviKI_1 | RGCY | 4 cut(s) | 45, 115, 149, 286 |
| DdeI | CTNAG | 3 cut(s) | 150, 162, 278 |
| Eam1104I | CTCTTC | 1 cut(s) | 68 |
| EarI | CTCTTC | 1 cut(s) | 68 |
| FaiI | YATR | 3 cut(s) | 207, 275, 322 |
| FblI | GTMKAC | 1 cut(s) | 269 |
| GsuI | CTGGAG | 1 cut(s) | 129 |
| HapII | CCGG | 1 cut(s) | 104 |
| HinfI | GANTC | 3 cut(s) | 100, 292, 299 |
| HpaII | CCGG | 1 cut(s) | 104 |
| Hpy166II | GTNNAC | 1 cut(s) | 270 |
| Hpy188I | TCNGA | 1 cut(s) | 51 |
| Hpy188III | TCNNGA | 4 cut(s) | 131, 211, 289, 296 |
| Hpy8I | GTNNAC | 1 cut(s) | 270 |
| HpyCH4III | ACNGT | 1 cut(s) | 125 |
| HpyCH4V | TGCA | 2 cut(s) | 37, 247 |
| HpyF3I | CTNAG | 3 cut(s) | 150, 162, 278 |
| LguI | GCTCTTC | 1 cut(s) | 68 |
| LpnPI | CCDG | 4 cut(s) | 23, 117, 159, 165 |
| LweI | GCATC | 1 cut(s) | 234 |
| MboII | GAAGA | 2 cut(s) | 85, 148 |
| MluCI | AATT | 1 cut(s) | 32 |
| MlyI | GAGTC | 3 cut(s) | 94, 293, 301 |
| MnlI | CCTC | 1 cut(s) | 268 |
| MseI | TTAA | 1 cut(s) | 57 |
| MspA1I | CMGCKG | 1 cut(s) | 149 |
| MspI | CCGG | 1 cut(s) | 104 |
| PciSI | GCTCTTC | 1 cut(s) | 68 |
| PleI | GAGTC | 3 cut(s) | 94, 293, 300 |
| PpsI | GAGTC | 3 cut(s) | 94, 293, 300 |
| PshBI | ATTAAT | 1 cut(s) | 57 |
| PsiI | TTATAA | 1 cut(s) | 322 |
| PvuII | CAGCTG | 1 cut(s) | 149 |
| SapI | GCTCTTC | 1 cut(s) | 68 |
| SaqAI | TTAA | 1 cut(s) | 57 |
| SchI | GAGTC | 3 cut(s) | 94, 293, 301 |
| SetI | ASST | 5 cut(s) | 42, 47, 151, 288, 320 |
| SfaNI | GCATC | 1 cut(s) | 234 |
| SmlI | CTYRAG | 2 cut(s) | 131, 287 |
| SmoI | CTYRAG | 2 cut(s) | 131, 287 |
| Sse9I | AATT | 1 cut(s) | 32 |
| SspI | AATATT | 1 cut(s) | 55 |
| TaaI | ACNGT | 1 cut(s) | 125 |
| TasI | AATT | 1 cut(s) | 32 |
| Tru1I | TTAA | 1 cut(s) | 57 |
| Tru9I | TTAA | 1 cut(s) | 57 |
| TscAI | CASTG | 1 cut(s) | 130 |
| TspRI | CASTG | 1 cut(s) | 130 |
| VspI | ATTAAT | 1 cut(s) | 57 |
| XmiI | GTMKAC | 1 cut(s) | 269 |