Component of the DNA topoisomerase VI involved in chromatin organization and progression of endoreduplication cycles. Relaxes both positive and negative superturns and exhibits a strong decatenase activity. The B subunit binds ATP
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 185 |
| AgsI | TTSAA | 1 cut(s) | 193 |
| AjiI | CACGTC | 1 cut(s) | 156 |
| AleI | CACNNNNGTG | 1 cut(s) | 158 |
| AluBI | AGCT | 2 cut(s) | 14, 124 |
| AluI | AGCT | 2 cut(s) | 14, 124 |
| Alw26I | GTCTC | 1 cut(s) | 203 |
| ApoI | RAATTY | 1 cut(s) | 185 |
| BccI | CCATC | 1 cut(s) | 157 |
| BcoDI | GTCTC | 1 cut(s) | 203 |
| BfaI | CTAG | 1 cut(s) | 220 |
| BmgBI | CACGTC | 1 cut(s) | 156 |
| BsaBI | GATNNNNATC | 1 cut(s) | 137 |
| Bse3DI | GCAATG | 1 cut(s) | 39 |
| Bse8I | GATNNNNATC | 1 cut(s) | 137 |
| BseJI | GATNNNNATC | 1 cut(s) | 137 |
| BseMI | GCAATG | 1 cut(s) | 39 |
| BseYI | CCCAGC | 1 cut(s) | 14 |
| BsmAI | GTCTC | 1 cut(s) | 203 |
| BsrDI | GCAATG | 1 cut(s) | 39 |
| BstMAI | GTCTC | 1 cut(s) | 203 |
| BstNSI | RCATGY | 1 cut(s) | 216 |
| BtrI | CACGTC | 1 cut(s) | 156 |
| CviAII | CATG | 1 cut(s) | 213 |
| CviJI | RGCY | 3 cut(s) | 14, 124, 167 |
| CviKI_1 | RGCY | 3 cut(s) | 14, 124, 167 |
| EcoRI | GAATTC | 1 cut(s) | 185 |
| FaeI | CATG | 1 cut(s) | 216 |
| FaiI | YATR | 4 cut(s) | 66, 74, 116, 214 |
| FatI | CATG | 1 cut(s) | 212 |
| FspBI | CTAG | 1 cut(s) | 220 |
| GsaI | CCCAGC | 1 cut(s) | 18 |
| Hin1II | CATG | 1 cut(s) | 216 |
| HinfI | GANTC | 2 cut(s) | 101, 110 |
| Hpy166II | GTNNAC | 1 cut(s) | 153 |
| Hpy188I | TCNGA | 2 cut(s) | 121, 132 |
| Hpy8I | GTNNAC | 1 cut(s) | 153 |
| Hpy99I | CGWCG | 1 cut(s) | 109 |
| HpyCH4IV | ACGT | 1 cut(s) | 155 |
| HpyCH4V | TGCA | 1 cut(s) | 216 |
| HpySE526I | ACGT | 1 cut(s) | 155 |
| Hsp92II | CATG | 1 cut(s) | 216 |
| LmnI | GCTCC | 1 cut(s) | 121 |
| LpnPI | CCDG | 2 cut(s) | 24, 30 |
| MaeI | CTAG | 1 cut(s) | 220 |
| MaeII | ACGT | 1 cut(s) | 155 |
| MboII | GAAGA | 2 cut(s) | 13, 181 |
| MluCI | AATT | 1 cut(s) | 185 |
| MlyI | GAGTC | 1 cut(s) | 119 |
| MnlI | CCTC | 2 cut(s) | 69, 138 |
| MslI | CAYNNNNRTG | 1 cut(s) | 158 |
| MspA1I | CMGCKG | 1 cut(s) | 14 |
| NlaIII | CATG | 1 cut(s) | 216 |
| NspI | RCATGY | 1 cut(s) | 216 |
| OliI | CACNNNNGTG | 1 cut(s) | 158 |
| PfeI | GAWTC | 1 cut(s) | 101 |
| PflFI | GACNNNGTC | 1 cut(s) | 109 |
| PleI | GAGTC | 1 cut(s) | 118 |
| PpsI | GAGTC | 1 cut(s) | 118 |
| PspFI | CCCAGC | 1 cut(s) | 14 |
| PsyI | GACNNNGTC | 1 cut(s) | 109 |
| PvuII | CAGCTG | 1 cut(s) | 14 |
| RseI | CAYNNNNRTG | 1 cut(s) | 158 |
| SchI | GAGTC | 1 cut(s) | 119 |
| SetI | ASST | 4 cut(s) | 16, 126, 158, 221 |
| SgeI | CNNG | 8 cut(s) | 23, 27, 46, 57, 95, 166, 180, 208 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 158 |
| Sse9I | AATT | 1 cut(s) | 185 |
| SspMI | CTAG | 1 cut(s) | 220 |
| TaiI | ACGT | 1 cut(s) | 158 |
| TaqI | TCGA | 1 cut(s) | 104 |
| TasI | AATT | 1 cut(s) | 185 |
| TfiI | GAWTC | 1 cut(s) | 101 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 1 cut(s) | 122 |
| Tth111I | GACNNNGTC | 1 cut(s) | 109 |
| XapI | RAATTY | 1 cut(s) | 185 |
| XceI | RCATGY | 1 cut(s) | 216 |
| XspI | CTAG | 1 cut(s) | 220 |