Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0001465

Physical Location & Seq

Genomic Coordinates Forward (+)

64905919 .. 64909789

Gene Structure 3871 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG488100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1692 bp

ATGATCCAGAGAGACCTTGATAACAAAAAGGCAGAGTTTTACATTGAGAGAGCAAAACTTGAAGCCAGCCATCTGGAGTCTTGTGAACCTCTGTACCTCCAGCGATACGATATTGTGAATGGTGTGGTTAAAGATACTGGAGTTACTACTGATGCTGTAAATCAAGAGGAAAATGCTGCCCGAGGTACATCAGTGACCCAGACCAAAATACAGCTAAAAGGGAAAGGGGTGCCTAATTTCTGGTTATATGCTTTAAAAGGCTGTGACACCGTAGATGACGAGATTACGGATCAAGATGAAGGAGCTCTCAAACATCTTACAGACATCAAATGGTCCAGGATACAGAAAGGGTTCAGACTTGAATTCCATTTTAGCACTAACCCCTTCTTTGAAAACTCTCTGTTGACCAAGACGTATCACATGACTGATGAAGATGAATCCTTTCTAGAACGTGCAATAGGGACAGAGATTACATGGCGTCCGGAAAAATGCTTGACACAGGAGCTCGCTGTGAAATCTGGGAAGGGATCAAAGAACGCTAAGCCAATGACTAATAAAAGCTTCTTCTGCTTTTTCAGTCCACCTAACGTGCCAATGAACTTGAAAGATAAGAATGATAAAGTTGAGATCTTAAATCTGAGATACCATGTGTTAATGGATCTTTTCTTGGGGTTGACCATTCGAGATGAGATCATCCCACATGCCATTTCATGGTTTACGGGGGAGGCTGCACTGGAGGAGGAGACCCAAATATCTGCACAGCACTCCAATTTCCTTGAGAATCTAGCAAAAAATGTCAGAGAGTGTGTTGAAAGCCTTAGACTGATCCAGGAAAAATATGATGCATTGAAAGCAAAGTTTGACGAGGATAGAACATCAATTCAAGCAAAATATCGTCAATCGTGTCAACTTTTGTATGAACAGCGATACAATATCATAAATGATGGAGAGAGTATCGATCCTGAAGCAGATGGAGTTGGTAGTGATTCTGAAGAGGAAGCATTCGTCGATGAGAAAGGGGTGCCTAACTTCTGGCTCTATGCTTTGCAAAACAATGACTATATAGACAATGAGATCACTTATCGTGATGAACAAGCTCTAAAATATCTTAAAGACATCAAGTGTTCCCCCATAGATGACTCCAAGGGATTCAAACTTGACTTCTGCTTTAAAGCCAATCCCTTCTTTAAAAACTCTGTCTTGACCAAGACATATCGCATGGTTGATGAGGATAGATCCCTTGTGAAGGATATAAAAGGGACTGAGGTTGAATGGTATCCCGCAAGATGCTTGACACAGATACCCGTTAAGAAGAAACCCGAGACACGATCGGAGAATGCGAAGTCAATCACTACAACTGAAGATTGTGATAGTTTCTTCTCCTTTTTCAGTGCACTTGAAGTAAATGATACCTATAAGCGGACAAATCAGGTGGCGCGTGATTTTAAGATCGGGTCAATTATTCGAGATCAGATTATCCCCCATGCAGTTTCATGGTTTACAGGGGAGGCTATTGAGAGAGATGACTCGTCAGATGATGAAGACCGATACCTTGGACTTGAAGCTCAATTTGATGATGAAGACGATGAAGATGATGATGACGATGATGATTATGAAGACCTTGATGATGGATTAGACTCTGATGGATGCTCTGAGGACGAAGAAGACATGGAAAATTACAATTATTATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAP

Protein Analysis

563

Amino Acids

65.04

Weight (kDa)

4.5

Isoelectric Point (pI)

31.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAP	PF00956	2 - 242	9.1e-62	Nucleosome assembly protein (NAP)
NAP	PF00956	270 - 503	1.4e-63	Nucleosome assembly protein (NAP)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001465)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g32980 FvH4_2g32990 FvH4_2g33010 FvH4_2g33010 FvH4_3g06070 FvH4_3g06070 FvH4_3g08121 FvH4_4g31040 FvH4_5g10050 FvH4_5g10050 FvH4_5g10060 FvH4_7g30720
rosa_chinensis	RchiOBHm_Chr5g0010081 RchiOBHm_Chr5g0010091 RchiOBHm_Chr5g0084011
rosa_laevigata	RLG00000004717 RLG00000010542 RLG00000011121 RLG00000026384 RLG00000026388 RLG00000031724
rosa_multiflora	Rmu_sc0000898.1_g000020 Rmu_sc0001192.1_g000048 Rmu_sc0003764.1_g000005 Rmu_ssc0000114.1_g000036
rosa_roxburghii	Rroxscaffold_3G00249040 Rroxscaffold_3G00266610 Rroxscaffold_4G00280020
rosa_rugosa	Rorug02G0380100 Rorug04G0449100 Rorug04G0449100 Rorug06G0489300
rosa_samantha	Rh1AG436800 Rh1CG406400 Rh2AG329500 Rh4AG189100 Rh4BG345900 Rh5AG280500 Rh5AG529400 Rh5CG087600 Rh5DG185000 Rh5DG374500 Rh6BG152500 Rh6BG169300 Rh6CG501800 Rh6DG136700 Rh6DG225600 Rh6DG488100 Rh7CG095300 Rh7DG400300
rosa_wichuraiana	Rw2G017690 Rw4G016130

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	229, 1021
AccB7I	CCANNNNNTGG	1 cut(s)	711
AccII	CGCG	1 cut(s)	1438
AccIII	TCCGGA	1 cut(s)	481
AciI	CCGC	2 cut(s)	1281, 1420
AclWI	GGATC	6 cut(s)	297, 535, 666, 820, 953, 1230
AcsI	RAATTY	1 cut(s)	362
AcuI	CTGAAG	3 cut(s)	984, 1011, 1380
AcyI	GRCGYC	1 cut(s)	478
AfaI	GTAC	2 cut(s)	95, 187
AfiI	CCNNNNNNNGG	3 cut(s)	711, 1246, 1419
AjnI	CCWGG	2 cut(s)	335, 828
AluBI	AGCT	6 cut(s)	214, 305, 505, 561, 1097, 1565
AluI	AGCT	6 cut(s)	214, 305, 505, 561, 1097, 1565
Alw21I	GWGCWC	3 cut(s)	307, 507, 1396
Alw26I	GTCTC	3 cut(s)	6, 737, 1316
Alw44I	GTGCAC	1 cut(s)	1392
AlwI	GGATC	6 cut(s)	297, 535, 666, 820, 953, 1230
Ama87I	CYCGRG	2 cut(s)	180, 1319
Aor13HI	TCCGGA	1 cut(s)	481
ApaLI	GTGCAC	1 cut(s)	1392
ApeKI	GCWGC	2 cut(s)	176, 728
ApoI	RAATTY	1 cut(s)	362
Asp700I	GAANNNNTTC	2 cut(s)	350, 441
AspLEI	GCGC	1 cut(s)	1438
AspS9I	GGNCC	1 cut(s)	333
AvaI	CYCGRG	2 cut(s)	180, 1319
AvaII	GGWCC	1 cut(s)	333
BaeGI	GKGCMC	1 cut(s)	1396
BaeI	ACNNNNGTAYC	6 cut(s)	77, 110, 126, 159, 177, 210
BanI	GGYRCC	2 cut(s)	229, 1021
BanII	GRGCYC	2 cut(s)	307, 507
BbsI	GAAGAC	4 cut(s)	1548, 1587, 1623, 1671
Bbv12I	GWGCWC	3 cut(s)	307, 507, 1396
BbvI	GCAGC	2 cut(s)	163, 715
BccI	CCATC	5 cut(s)	78, 938, 965, 1622, 1637
BciT130I	CCWGG	2 cut(s)	337, 830
BciVI	GTATCC	2 cut(s)	333, 1287
BcoDI	GTCTC	3 cut(s)	6, 737, 1316
BfaI	CTAG	2 cut(s)	446, 785
BfuI	GTATCC	2 cut(s)	333, 1287
BglII	AGATCT	1 cut(s)	627
BisI	GCNGC	2 cut(s)	177, 729
BlpI	GCTNAGC	1 cut(s)	540
BlsI	GCNGC	2 cut(s)	178, 730
Bme1390I	CCNGG	2 cut(s)	337, 830
Bme18I	GGWCC	1 cut(s)	333
BmeT110I	CYCGRG	2 cut(s)	180, 1319
BmgT120I	GGNCC	1 cut(s)	333
BmiI	GGNNCC	2 cut(s)	231, 1023
BmrFI	CCNGG	2 cut(s)	337, 830
BmsI	GCATC	4 cut(s)	142, 832, 1277, 1637
BpiI	GAAGAC	4 cut(s)	1548, 1587, 1623, 1671
BpmI	CTGGAG	4 cut(s)	83, 95, 159, 755
Bpu1102I	GCTNAGC	1 cut(s)	540
BpuEI	CTTGAG	1 cut(s)	797
Bsa29I	ATCGAT	1 cut(s)	957
BsaHI	GRCGYC	1 cut(s)	478
BsaI	GGTCTC	2 cut(s)	6, 737
BsaJI	CCNNGG	3 cut(s)	181, 1143, 1552
BsaWI	WCCGGW	1 cut(s)	481
Bsc4I	CCNNNNNNNGG	3 cut(s)	711, 1246, 1419
Bse1I	ACTGG	2 cut(s)	142, 738
BseAI	TCCGGA	1 cut(s)	481
BseBI	CCWGG	2 cut(s)	337, 830
BseCI	ATCGAT	1 cut(s)	957
BseDI	CCNNGG	3 cut(s)	181, 1143, 1552
BseGI	GGATG	2 cut(s)	693, 1652
BseLI	CCNNNNNNNGG	3 cut(s)	711, 1246, 1419
BseMII	CTCAG	3 cut(s)	629, 1254, 1644
BseNI	ACTGG	2 cut(s)	142, 738
BseRI	GAGGAG	2 cut(s)	752, 755
BseSI	GKGCMC	1 cut(s)	1396
BseXI	GCAGC	2 cut(s)	163, 715
BsgI	GTGCAG	2 cut(s)	714, 741
Bsh1236I	CGCG	1 cut(s)	1438
Bsh1285I	CGRYCG	1 cut(s)	1331
BshNI	GGYRCC	2 cut(s)	229, 1021
BshVI	ATCGAT	1 cut(s)	957
BsiEI	CGRYCG	1 cut(s)	1331
BsiHKAI	GWGCWC	3 cut(s)	307, 507, 1396
BsiHKCI	CYCGRG	2 cut(s)	180, 1319
BsiSI	CCGG	1 cut(s)	482
BslFI	GGGAC	2 cut(s)	475, 1273
BslI	CCNNNNNNNGG	3 cut(s)	711, 1246, 1419
BsmAI	GTCTC	3 cut(s)	6, 737, 1316
BsmFI	GGGAC	2 cut(s)	475, 1273
BsmI	GAATGC	2 cut(s)	1001, 1342
Bso31I	GGTCTC	2 cut(s)	6, 737
BsoBI	CYCGRG	2 cut(s)	180, 1319
Bsp1286I	GDGCHC	3 cut(s)	307, 507, 1396
Bsp13I	TCCGGA	1 cut(s)	481
Bsp1720I	GCTNAGC	1 cut(s)	540
BspACI	CCGC	2 cut(s)	1281, 1420
BspCNI	CTCAG	3 cut(s)	630, 1255, 1645
BspDI	ATCGAT	1 cut(s)	957
BspEI	TCCGGA	1 cut(s)	481
BspFNI	CGCG	1 cut(s)	1438
BspLI	GGNNCC	2 cut(s)	231, 1023
BspPI	GGATC	6 cut(s)	297, 535, 666, 820, 953, 1230
BspT107I	GGYRCC	2 cut(s)	229, 1021
BspTNI	GGTCTC	2 cut(s)	6, 737
BsrI	ACTGG	2 cut(s)	142, 738
BssECI	CCNNGG	3 cut(s)	181, 1143, 1552
BssNI	GRCGYC	1 cut(s)	478
BssT1I	CCWWGG	2 cut(s)	1143, 1552
Bst2UI	CCWGG	2 cut(s)	337, 830
Bst4CI	ACNGT	1 cut(s)	271
Bst6I	CTCTTC	1 cut(s)	987
BstACI	GRCGYC	1 cut(s)	478
BstC8I	GCNNGC	2 cut(s)	67, 507
BstDEI	CTNAG	5 cut(s)	540, 638, 818, 1263, 1653
BstENI	CCTNNNNNAGG	1 cut(s)	1244
BstF5I	GGATG	2 cut(s)	693, 1652
BstFNI	CGCG	1 cut(s)	1438
BstHHI	GCGC	1 cut(s)	1438
BstMAI	GTCTC	3 cut(s)	6, 737, 1316
BstMCI	CGRYCG	1 cut(s)	1331
BstMWI	GCNNNNNNNGC	2 cut(s)	567, 851
BstNI	CCWGG	2 cut(s)	337, 830
BstNSI	RCATGY	1 cut(s)	704
BstSCI	CCNGG	2 cut(s)	335, 828
BstSLI	GKGCMC	1 cut(s)	1396
BstUI	CGCG	1 cut(s)	1438
BstV1I	GCAGC	2 cut(s)	163, 715
BstV2I	GAAGAC	4 cut(s)	1548, 1587, 1623, 1671
BstX2I	RGATCY	3 cut(s)	627, 658, 1235
BstXI	CCANNNNNNTGG	1 cut(s)	73
BstYI	RGATCY	3 cut(s)	627, 658, 1235
Bsu15I	ATCGAT	1 cut(s)	957
BsuI	GTATCC	2 cut(s)	333, 1287
BsuTUI	ATCGAT	1 cut(s)	957
BtsCI	GGATG	2 cut(s)	693, 1652
BtsIMutI	CAGTG	3 cut(s)	198, 731, 1396
Cac8I	GCNNGC	2 cut(s)	67, 507
CfoI	GCGC	1 cut(s)	1438
Cfr13I	GGNCC	1 cut(s)	333
ClaI	ATCGAT	1 cut(s)	957
CseI	GACGC	1 cut(s)	467
Csp6I	GTAC	2 cut(s)	94, 186
CspCI	CAANNNNNGTGG	2 cut(s)	1413, 1448
CviAII	CATG	9 cut(s)	421, 474, 647, 701, 711, 1219, 1484, 1494, 1669
CviQI	GTAC	2 cut(s)	94, 186
DdeI	CTNAG	5 cut(s)	540, 638, 818, 1263, 1653
DraI	TTTAAA	3 cut(s)	255, 1171, 1189
Eam1104I	CTCTTC	1 cut(s)	987
EarI	CTCTTC	1 cut(s)	987
Ecl136II	GAGCTC	2 cut(s)	305, 505
Eco130I	CCWWGG	2 cut(s)	1143, 1552
Eco24I	GRGCYC	2 cut(s)	307, 507
Eco31I	GGTCTC	2 cut(s)	6, 737
Eco47I	GGWCC	1 cut(s)	333
Eco53kI	GAGCTC	2 cut(s)	305, 505
Eco57I	CTGAAG	3 cut(s)	984, 1011, 1380
Eco88I	CYCGRG	2 cut(s)	180, 1319
EcoICRI	GAGCTC	2 cut(s)	305, 505
EcoNI	CCTNNNNNAGG	1 cut(s)	1244
EcoRI	GAATTC	1 cut(s)	362
EcoRII	CCWGG	2 cut(s)	335, 828
EcoT14I	CCWWGG	2 cut(s)	1143, 1552
EcoT22I	ATGCAT	1 cut(s)	847
EcoT38I	GRGCYC	2 cut(s)	307, 507
ErhI	CCWWGG	2 cut(s)	1143, 1552
FaeI	CATG	9 cut(s)	424, 477, 650, 704, 714, 1222, 1487, 1497, 1672
FaqI	GGGAC	2 cut(s)	475, 1273
FatI	CATG	9 cut(s)	420, 473, 646, 700, 710, 1218, 1483, 1493, 1668
FauI	CCCGC	1 cut(s)	1288
Fnu4HI	GCNGC	2 cut(s)	177, 729
FokI	GGATG	2 cut(s)	680, 1659
FriOI	GRGCYC	2 cut(s)	307, 507
Fsp4HI	GCNGC	2 cut(s)	177, 729
FspBI	CTAG	2 cut(s)	446, 785
GlaI	GCGC	1 cut(s)	1437
GluI	GCNGC	2 cut(s)	177, 729
GsuI	CTGGAG	4 cut(s)	83, 95, 159, 755
HapII	CCGG	1 cut(s)	482
HgaI	GACGC	1 cut(s)	467
HhaI	GCGC	1 cut(s)	1438
Hin1I	GRCGYC	1 cut(s)	478
Hin1II	CATG	9 cut(s)	424, 477, 650, 704, 714, 1222, 1487, 1497, 1672
Hin6I	GCGC	1 cut(s)	1436
HinP1I	GCGC	1 cut(s)	1436
HincII	GTYRAC	3 cut(s)	405, 675, 908
HindII	GTYRAC	3 cut(s)	405, 675, 908
HindIII	AAGCTT	1 cut(s)	559
HinfI	GANTC	8 cut(s)	77, 437, 781, 986, 1139, 1149, 1526, 1637
HpaII	CCGG	1 cut(s)	482
Hpy166II	GTNNAC	8 cut(s)	86, 405, 581, 675, 717, 908, 1394, 1500
Hpy188I	TCNGA	9 cut(s)	356, 639, 800, 991, 1333, 1473, 1534, 1642, 1654
Hpy8I	GTNNAC	8 cut(s)	86, 405, 581, 675, 717, 908, 1394, 1500
Hpy99I	CGWCG	1 cut(s)	1010
HpyAV	CCTTC	5 cut(s)	293, 394, 517, 1192, 1240
HpyCH4III	ACNGT	1 cut(s)	271
HpyCH4IV	ACGT	3 cut(s)	413, 451, 588
HpyCH4V	TGCA	7 cut(s)	455, 731, 758, 845, 1048, 1394, 1487
HpyF10VI	GCNNNNNNNGC	2 cut(s)	567, 851
HpyF3I	CTNAG	5 cut(s)	540, 638, 818, 1263, 1653
HpySE526I	ACGT	3 cut(s)	413, 451, 588
Hsp92I	GRCGYC	1 cut(s)	478
Hsp92II	CATG	9 cut(s)	424, 477, 650, 704, 714, 1222, 1487, 1497, 1672
HspAI	GCGC	1 cut(s)	1436
Kpn2I	TCCGGA	1 cut(s)	481
LmnI	GCTCC	2 cut(s)	302, 502
Lsp1109I	GCAGC	2 cut(s)	163, 715
LweI	GCATC	4 cut(s)	142, 832, 1277, 1637
MaeI	CTAG	2 cut(s)	446, 785
MaeII	ACGT	3 cut(s)	413, 451, 588
MaeIII	GTNAC	3 cut(s)	142, 193, 263
MflI	RGATCY	3 cut(s)	627, 658, 1235
MhlI	GDGCHC	3 cut(s)	307, 507, 1396
MluCI	AATT	8 cut(s)	235, 362, 769, 879, 1458, 1568, 1675, 1681
MlyI	GAGTC	4 cut(s)	86, 1133, 1520, 1631
Mph1103I	ATGCAT	1 cut(s)	847
MroI	TCCGGA	1 cut(s)	481
MroXI	GAANNNNTTC	2 cut(s)	350, 441
MspI	CCGG	1 cut(s)	482
MspR9I	CCNGG	2 cut(s)	337, 830
Mva1269I	GAATGC	2 cut(s)	1001, 1342
MvaI	CCWGG	2 cut(s)	337, 830
MvnI	CGCG	1 cut(s)	1438
MwoI	GCNNNNNNNGC	2 cut(s)	567, 851
NlaIII	CATG	9 cut(s)	424, 477, 650, 704, 714, 1222, 1487, 1497, 1672
NlaIV	GGNNCC	2 cut(s)	231, 1023
NmuCI	GTSAC	2 cut(s)	193, 263
NsiI	ATGCAT	1 cut(s)	847
NspI	RCATGY	1 cut(s)	704
PcsI	WCGNNNNNNNCGW	1 cut(s)	1337
PctI	GAATGC	2 cut(s)	1001, 1342
PdmI	GAANNNNTTC	2 cut(s)	350, 441
PfeI	GAWTC	4 cut(s)	437, 781, 986, 1149
PflMI	CCANNNNNTGG	1 cut(s)	711
PfoI	TCCNGGA	2 cut(s)	335, 828
PkrI	GCNGC	2 cut(s)	178, 730
Ple19I	CGATCG	1 cut(s)	1331
PleI	GAGTC	4 cut(s)	85, 1133, 1520, 1631
PpsI	GAGTC	4 cut(s)	85, 1133, 1520, 1631
Psp124BI	GAGCTC	2 cut(s)	307, 507
Psp6I	CCWGG	2 cut(s)	335, 828
PspGI	CCWGG	2 cut(s)	335, 828
PspN4I	GGNNCC	2 cut(s)	231, 1023
PspPI	GGNCC	1 cut(s)	333
PsuI	RGATCY	3 cut(s)	627, 658, 1235
PvuI	CGATCG	1 cut(s)	1331
RsaI	GTAC	2 cut(s)	95, 187
RsaNI	GTAC	2 cut(s)	94, 186
SacI	GAGCTC	2 cut(s)	307, 507
SatI	GCNGC	2 cut(s)	177, 729
Sau96I	GGNCC	1 cut(s)	333
SchI	GAGTC	4 cut(s)	86, 1133, 1520, 1631
ScrFI	CCNGG	2 cut(s)	337, 830
SduI	GDGCHC	3 cut(s)	307, 507, 1396
SfaNI	GCATC	4 cut(s)	142, 832, 1277, 1637
SinI	GGWCC	1 cut(s)	333
SmlI	CTYRAG	1 cut(s)	776
SmoI	CTYRAG	1 cut(s)	776
Sse9I	AATT	8 cut(s)	235, 362, 769, 879, 1458, 1568, 1675, 1681
SsiI	CCGC	2 cut(s)	1281, 1420
SspMI	CTAG	2 cut(s)	446, 785
SstI	GAGCTC	2 cut(s)	307, 507
StyD4I	CCNGG	2 cut(s)	335, 828
StyI	CCWWGG	2 cut(s)	1143, 1552
TaaI	ACNGT	1 cut(s)	271
TaiI	ACGT	3 cut(s)	416, 454, 591
TaqI	TCGA	4 cut(s)	682, 957, 1008, 1465
TaqII	GACCGA	1 cut(s)	1560
TasI	AATT	8 cut(s)	235, 362, 769, 879, 1458, 1568, 1675, 1681
TfiI	GAWTC	4 cut(s)	437, 781, 986, 1149
TscAI	CASTG	3 cut(s)	198, 738, 1396
TseFI	GTSAC	2 cut(s)	193, 263
TseI	GCWGC	2 cut(s)	176, 728
Tsp45I	GTSAC	2 cut(s)	193, 263
TspGWI	ACGGA	1 cut(s)	302
TspRI	CASTG	3 cut(s)	198, 738, 1396
Van91I	CCANNNNNTGG	1 cut(s)	711
VneI	GTGCAC	1 cut(s)	1392
VpaK11BI	GGWCC	1 cut(s)	333
XagI	CCTNNNNNAGG	1 cut(s)	1244
XapI	RAATTY	1 cut(s)	362
XbaI	TCTAGA	1 cut(s)	445
XceI	RCATGY	1 cut(s)	704
XmnI	GAANNNNTTC	2 cut(s)	350, 441
XspI	CTAG	2 cut(s)	446, 785
Zsp2I	ATGCAT	1 cut(s)	847

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.